ssfa2 cDNA ORF clone, Danio rerio(zebrafish)

The following ssfa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ssfa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa60372 XM_017357843.2
Latest version!
Danio rerio sperm specific antigen 2 (ssfa2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa60372 XM_021479016.1
Latest version!
Danio rerio sperm specific antigen 2 (ssfa2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
View Old Accession Versions >>
ODa60372 XM_017357843.1 Danio rerio sperm specific antigen 2 (ssfa2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa60372
    Clone ID Related Accession (Same CDS sequence) XM_017357843.2 , XM_017357843.1 , XM_021479016.1
    Accession Version XM_017357843.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm-specific antigen 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) and transcript sequence (GDQQ01039019.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 209 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAGG TTGAATCGCC CCCTGCCAGA TTCAAGAGGA AGGCCTGGGC TCAGAGCCGA 
    GACTCCTGGC AGGCGTCTGA GTCTCAGGAC CAGGGCGCAA AAGAGCCGCT GTCTGCCCAG
    CAGCCTATGG AGGAGAAGAC TTCCACAGCC TCAGAAGCAG GTCGTAATCC AAATCAAGTG
    GAGAACTGGC TCAGGACCTG CAGGACTCCT CTCGGGGCCT CTTTGGATGA CCAGAGCAGC
    AGTCCGTCCA GAGGGCCGCT GAAGAATGGC TGTAGTTTTG AAGATGATCT GTCACTGGGA
    GCTGAGGCTA ATCACCTCCA GAACAGAAAA ACAGAGGCCG ATGGCTATGA AATGCACGCC
    AAGGCCAAAA GGAGCCAATT CAAACAGAAG GGCAGAAGTA TGAACTCCAC GGGTTCTGGA
    AAGAGCAGCG CCACAGTGTC CAGTGTTTCA GAATTGCTGG ACCTGTACGA GGAGGATCCA
    GAAGAGATCT TGTACAATCT TGGATTTGGG CGGGAAGAGC CAGACATTGC CTCTAAAATC
    CCTTCTCGTT TCTTCAACGC GTCGTCTAAC GCTAAAGGCA TCGACATTAA AGTTTACCTG
    GGAGCCCAGA TTCAGCGCAT GGAGATAGAG AACCCAAACT ACGCTCTGAC AAGTCGTTTC
    CGTCAGACCG AAGTCTTGGC TACAGTGGCG AACGTCCTCT CCGATATTTA TTCCCACGTA
    TCTGGAAAAC CCACAGATAA AATCGGCAAT GGTGACATGG AGACCAATGT GCCTAATCCG
    CTAAAAAAGA GAAGCTCTGC TGGAAACTTT GCTAAAATTC TCAAGCGCAA CTTTACGAAG
    CAGAACCTGC TGGCCTCCAT CTCGGATGGA GAAACCAAAC AGCCTAACAA TGAGAGCTCC
    AACATGCGGG ACGTTCAGAC GGATACAGAC CACAAAACAC AACAGAAGAG CTTAAAGAAA
    AAGGATTCAT CTTTGGCGAC CGTGACGGAG GAGGCTCAGC AGAATATCTC TGATGTTTCG
    ATCAGCGCAG ATTTTACGTT GACCAATGGC GAGTCCACAG ACTTGCCTCT GCCTCCGGTC
    CTAGTGAGCA TCAGTCAGGC GTCTGAAGAG AACGATACCT CTGAATTGAA GGTAAACGGG
    TGCCATGAGG CCATCACGTC CACCCCAGAT AAAGAGCCGT CCGGCCTGCT GTCAAACCCA
    CACATCATCC ACTTGCTCGA CCAGCCCAGA GACTCCTTTG AGATGGAGGA GTTGCAGAGT
    AATGAGGATG AAACTCTTCC TGGTCCAGGC GGCATGTCTC GATCTGACAG CGAGCGGTTA
    TTGAGGACAG CCAGCCAGCA GTCTGACAGC AGCGGGTTTG CTGAGGATCC ATCCACAGAC
    GGTTCGGGTA ATCACCTGAA GGTACAAGAG AGCTCAGACA GCTGTGACAG TGAAACCACA
    GTAACATCGC ATGCTGAAGT CAGCACACCC ATAGCATTGG AATACCCCAA GTTTGACAGA
    TTCCCAAGTG AGGAAGTTCA GCTCTACCGA AGCACAACTA CCCAATCTGC AACCAATCAA
    GAAGTCCATG ACATCCCCAA TCTCCAAATT CCCAATCCAA ACTTGGACGA AATTAGCACC
    AATGGACTGC TTCTTTCTCC TGACTCAGAT AGGGCATCTG TCATTGAGTC CGTTTCCACC
    ATAGACTCTC AAGAAGGAAA TTCAAACATG ATGATAGATC ATACAGTGGA CTCCATACTG
    AAAGTAGCTC ATGAGGGCAA CTTATCCCAA CCTAGTCTGG GCATAAATCA AAAGTCTCCT
    GAAGATCTAC TGGAGCAAGA CTCCTCATCA GAGCTTCCAG ACTCATCAGA TCTTAAGCAG
    TCCCACCTTA CAGCTCAAAG GAGATCCATG GCACTCCACA CAGGCCATCA AAGATCATCC
    TCAATGGAGG AGGAGCGTCC TGGCCGAGTG TGGGTGAGGG ATCGGGGCCT GCTGAGGGAG
    AGCCAGGAGA GGAATCCTTT AAGGAGATCC AGCTCCCTCC CATTATCTTC GCTCAACCCG
    GCTCGCGTGG TCACCTCCAT GCGCATCCAG CTTGGAAAAG GTTCAGTCAA GCACTGTACC
    CCGCCATCAT ACACATACCG CTATGAGGAA GACCATTGCA GTGACCGAGA AGAAGTAGAC
    TCCATCTTGG AGGAGGATGA AGTGGATGGT TCGGGTGTCC AATCTGCGCT CCCGAACTCT
    GGATCAAAGA TGAACAGAGG AACTTCACCA CCACCTCCAC CACACCCTCT CGATATACCC
    CAACATCTGG CTCGCTCCTC CACCTCGCTT TATAGCGTTC CCGCTGACTG GCCGCACAGG
    CCTTTGGGTG AAGGTACAAA CTGGAGCGCT AGCGTCCCTA ACCTTTCTCA TTTTACAGCA
    CCATCCCACT TTCCTCATCA CGGAACCTTC TACTCTCCGC AACACGGAGT GTCCTACAAT
    TCCTCATACC CATCTAGGCT TCCCTTCCCA CAGTACCAAA ACTCGCCTTG TCCTCCACCG
    CTTCAAAGCA ACACATTTGT TCCACCACAG GGGGCACACT TTACACCTCA GAACAATATG
    CATTATGCTC ACCCGCACAG TGCTCCCTAC AACCCATTCT ATCCATCTAA TAACATTCCT
    CAAGGAACAC CCTATGACCC ATCGTTGAAT ATGCAAGGCA ACCCGTACCC AAACCATGTC
    CCTCTTGCTC AATCCCACAG CGCCCCTTAC AGTTCGGGAT ACAATGGTTC ACCTCATCAC
    CCTGCTGCTG TTTACAGCCA TCCGTATTAT CCCTCACCCG CCTACCCTTT CTCATTTGGG
    ACGCCTCCTG CCCCTACCCC TGCGATGGGC AGCACAGAGA TGCAGCTCAG GAGGGTCCTG
    CATGACATTC GTGGGACAAT GCAAAATCTT GGTCAGAGCT CATCCGTGCA TGGAGGAGAC
    ACACCCATGG ACAGGATCGG CACACCGCGC TCCGTCCAAC CGCTTTACGA GGAGCTCCAG
    ATGCGGCGCA GGAGTCTGAA TGTCTTCCGG ACTCAGATGA TGGATCTTGA ACTGGCCCTC
    ATGAGACAGC AGTCTTTGGT CTACCAGCAT CTCAGTCCAG AAGAGAGGCG GGAGGCAGAG
    CAGCTTCAGA CGCTGCGGAC TGCAGTCAGG CAGGAGCTGC AGGAGCTGGA GCTGCAATTG
    GAGGATCGGC TGCTCTCTAT CAATGAACAA ATGCGCAACA GCTCGCAACA CATCGGCTTC
    TACCGACACC ACATGGGCAT GCACAGAGGT CACAGCATGG AAAGCCTGTC TAATTCTTCA
    GCACTGAGGG CCATGGAGCC GGTGACGGAT CTTCTGAAGG AGCAATTGTA CCTTCAGTCT
    GAGCTGGCAT ATGAGGCTGC AACCCCCAGT TCAGGCCGTT CATCGAGGGC GTCCAGTCCT
    GTCAGATTCA ACCGAACACT GGAGTCTTGC TCTCCATCCC CACAGAGAGC CAGCGTTTAC
    CGGGCCACCC TCAACCTCAT GCCCACCCTA CCTCCACGGC CGGGGATGGA AGCTCCTCGG
    GATGTTTCTC CTGAGAGGAT GGAGTCAAAC CCGACACCCA CTTCTCTGGA GGTCAGCAGA
    GATGAGGACG AGGTGTCACT GGATGGTCAT GGGAGCAGGA CGGCCGAAGA TTGCAGTCCC
    ACAGAGTGGA GGAGTAGAAG TAGAGGAAAT GGAGACCTGC AGCATCTCAT AAAGGAGATT
    AAAGAAAGCA TCGCACATGA AATCCGGCAG GAGATCGTGA ATGAGCTTCT GGCTGCCGTG
    TCGCCACACA GATCTCCTCT TCCAGCAAGG CAGCCCCCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213332.1
    CDS161..3985
    Translation

    Target ORF information:

    RefSeq Version XM_017357843.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm specific antigen 2 (ssfa2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357843.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAGG TTGAATCGCC CCCTGCCAGA TTCAAGAGGA AGGCCTGGGC TCAGAGCCGA 
    GACTCCTGGC AGGCGTCTGA GTCTCAGGAC CAGGGCGCAA AAGAGCCGCT GTCTGCCCAG
    CAGCCTATGG AGGAGAAGAC TTCCACAGCC TCAGAAGCAG GTCGTAATCC AAATCAAGTG
    GAGAACTGGC TCAGGACCTG CAGGACTCCT CTCGGGGCCT CTTTGGATGA CCAGAGCAGC
    AGTCCGTCCA GAGGGCCGCT GAAGAATGGC TGTAGTTTTG AAGATGATCT GTCACTGGGA
    GCTGAGGCTA ATCACCTCCA GAACAGAAAA ACAGAGGCCG ATGGCTATGA AATGCACGCC
    AAGGCCAAAA GGAGCCAATT CAAACAGAAG GGCAGAAGTA TGAACTCCAC GGGTTCTGGA
    AAGAGCAGCG CCACAGTGTC CAGTGTTTCA GAATTGCTGG ACCTGTACGA GGAGGATCCA
    GAAGAGATCT TGTACAATCT TGGATTTGGG CGGGAAGAGC CAGACATTGC CTCTAAAATC
    CCTTCTCGTT TCTTCAACGC GTCGTCTAAC GCTAAAGGCA TCGACATTAA AGTTTACCTG
    GGAGCCCAGA TTCAGCGCAT GGAGATAGAG AACCCAAACT ACGCTCTGAC AAGTCGTTTC
    CGTCAGACCG AAGTCTTGGC TACAGTGGCG AACGTCCTCT CCGATATTTA TTCCCACGTA
    TCTGGAAAAC CCACAGATAA AATCGGCAAT GGTGACATGG AGACCAATGT GCCTAATCCG
    CTAAAAAAGA GAAGCTCTGC TGGAAACTTT GCTAAAATTC TCAAGCGCAA CTTTACGAAG
    CAGAACCTGC TGGCCTCCAT CTCGGATGGA GAAACCAAAC AGCCTAACAA TGAGAGCTCC
    AACATGCGGG ACGTTCAGAC GGATACAGAC CACAAAACAC AACAGAAGAG CTTAAAGAAA
    AAGGATTCAT CTTTGGCGAC CGTGACGGAG GAGGCTCAGC AGAATATCTC TGATGTTTCG
    ATCAGCGCAG ATTTTACGTT GACCAATGGC GAGTCCACAG ACTTGCCTCT GCCTCCGGTC
    CTAGTGAGCA TCAGTCAGGC GTCTGAAGAG AACGATACCT CTGAATTGAA GGTAAACGGG
    TGCCATGAGG CCATCACGTC CACCCCAGAT AAAGAGCCGT CCGGCCTGCT GTCAAACCCA
    CACATCATCC ACTTGCTCGA CCAGCCCAGA GACTCCTTTG AGATGGAGGA GTTGCAGAGT
    AATGAGGATG AAACTCTTCC TGGTCCAGGC GGCATGTCTC GATCTGACAG CGAGCGGTTA
    TTGAGGACAG CCAGCCAGCA GTCTGACAGC AGCGGGTTTG CTGAGGATCC ATCCACAGAC
    GGTTCGGGTA ATCACCTGAA GGTACAAGAG AGCTCAGACA GCTGTGACAG TGAAACCACA
    GTAACATCGC ATGCTGAAGT CAGCACACCC ATAGCATTGG AATACCCCAA GTTTGACAGA
    TTCCCAAGTG AGGAAGTTCA GCTCTACCGA AGCACAACTA CCCAATCTGC AACCAATCAA
    GAAGTCCATG ACATCCCCAA TCTCCAAATT CCCAATCCAA ACTTGGACGA AATTAGCACC
    AATGGACTGC TTCTTTCTCC TGACTCAGAT AGGGCATCTG TCATTGAGTC CGTTTCCACC
    ATAGACTCTC AAGAAGGAAA TTCAAACATG ATGATAGATC ATACAGTGGA CTCCATACTG
    AAAGTAGCTC ATGAGGGCAA CTTATCCCAA CCTAGTCTGG GCATAAATCA AAAGTCTCCT
    GAAGATCTAC TGGAGCAAGA CTCCTCATCA GAGCTTCCAG ACTCATCAGA TCTTAAGCAG
    TCCCACCTTA CAGCTCAAAG GAGATCCATG GCACTCCACA CAGGCCATCA AAGATCATCC
    TCAATGGAGG AGGAGCGTCC TGGCCGAGTG TGGGTGAGGG ATCGGGGCCT GCTGAGGGAG
    AGCCAGGAGA GGAATCCTTT AAGGAGATCC AGCTCCCTCC CATTATCTTC GCTCAACCCG
    GCTCGCGTGG TCACCTCCAT GCGCATCCAG CTTGGAAAAG GTTCAGTCAA GCACTGTACC
    CCGCCATCAT ACACATACCG CTATGAGGAA GACCATTGCA GTGACCGAGA AGAAGTAGAC
    TCCATCTTGG AGGAGGATGA AGTGGATGGT TCGGGTGTCC AATCTGCGCT CCCGAACTCT
    GGATCAAAGA TGAACAGAGG AACTTCACCA CCACCTCCAC CACACCCTCT CGATATACCC
    CAACATCTGG CTCGCTCCTC CACCTCGCTT TATAGCGTTC CCGCTGACTG GCCGCACAGG
    CCTTTGGGTG AAGGTACAAA CTGGAGCGCT AGCGTCCCTA ACCTTTCTCA TTTTACAGCA
    CCATCCCACT TTCCTCATCA CGGAACCTTC TACTCTCCGC AACACGGAGT GTCCTACAAT
    TCCTCATACC CATCTAGGCT TCCCTTCCCA CAGTACCAAA ACTCGCCTTG TCCTCCACCG
    CTTCAAAGCA ACACATTTGT TCCACCACAG GGGGCACACT TTACACCTCA GAACAATATG
    CATTATGCTC ACCCGCACAG TGCTCCCTAC AACCCATTCT ATCCATCTAA TAACATTCCT
    CAAGGAACAC CCTATGACCC ATCGTTGAAT ATGCAAGGCA ACCCGTACCC AAACCATGTC
    CCTCTTGCTC AATCCCACAG CGCCCCTTAC AGTTCGGGAT ACAATGGTTC ACCTCATCAC
    CCTGCTGCTG TTTACAGCCA TCCGTATTAT CCCTCACCCG CCTACCCTTT CTCATTTGGG
    ACGCCTCCTG CCCCTACCCC TGCGATGGGC AGCACAGAGA TGCAGCTCAG GAGGGTCCTG
    CATGACATTC GTGGGACAAT GCAAAATCTT GGTCAGAGCT CATCCGTGCA TGGAGGAGAC
    ACACCCATGG ACAGGATCGG CACACCGCGC TCCGTCCAAC CGCTTTACGA GGAGCTCCAG
    ATGCGGCGCA GGAGTCTGAA TGTCTTCCGG ACTCAGATGA TGGATCTTGA ACTGGCCCTC
    ATGAGACAGC AGTCTTTGGT CTACCAGCAT CTCAGTCCAG AAGAGAGGCG GGAGGCAGAG
    CAGCTTCAGA CGCTGCGGAC TGCAGTCAGG CAGGAGCTGC AGGAGCTGGA GCTGCAATTG
    GAGGATCGGC TGCTCTCTAT CAATGAACAA ATGCGCAACA GCTCGCAACA CATCGGCTTC
    TACCGACACC ACATGGGCAT GCACAGAGGT CACAGCATGG AAAGCCTGTC TAATTCTTCA
    GCACTGAGGG CCATGGAGCC GGTGACGGAT CTTCTGAAGG AGCAATTGTA CCTTCAGTCT
    GAGCTGGCAT ATGAGGCTGC AACCCCCAGT TCAGGCCGTT CATCGAGGGC GTCCAGTCCT
    GTCAGATTCA ACCGAACACT GGAGTCTTGC TCTCCATCCC CACAGAGAGC CAGCGTTTAC
    CGGGCCACCC TCAACCTCAT GCCCACCCTA CCTCCACGGC CGGGGATGGA AGCTCCTCGG
    GATGTTTCTC CTGAGAGGAT GGAGTCAAAC CCGACACCCA CTTCTCTGGA GGTCAGCAGA
    GATGAGGACG AGGTGTCACT GGATGGTCAT GGGAGCAGGA CGGCCGAAGA TTGCAGTCCC
    ACAGAGTGGA GGAGTAGAAG TAGAGGAAAT GGAGACCTGC AGCATCTCAT AAAGGAGATT
    AAAGAAAGCA TCGCACATGA AATCCGGCAG GAGATCGTGA ATGAGCTTCT GGCTGCCGTG
    TCGCCACACA GATCTCCTCT TCCAGCAAGG CAGCCCCCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60372
    Clone ID Related Accession (Same CDS sequence) XM_017357843.2 , XM_017357843.1 , XM_021479016.1
    Accession Version XM_017357843.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product sperm-specific antigen 2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) and transcript sequence (GDQQ01039019.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 209 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAGG TTGAATCGCC CCCTGCCAGA TTCAAGAGGA AGGCCTGGGC TCAGAGCCGA 
    GACTCCTGGC AGGCGTCTGA GTCTCAGGAC CAGGGCGCAA AAGAGCCGCT GTCTGCCCAG
    CAGCCTATGG AGGAGAAGAC TTCCACAGCC TCAGAAGCAG GTCGTAATCC AAATCAAGTG
    GAGAACTGGC TCAGGACCTG CAGGACTCCT CTCGGGGCCT CTTTGGATGA CCAGAGCAGC
    AGTCCGTCCA GAGGGCCGCT GAAGAATGGC TGTAGTTTTG AAGATGATCT GTCACTGGGA
    GCTGAGGCTA ATCACCTCCA GAACAGAAAA ACAGAGGCCG ATGGCTATGA AATGCACGCC
    AAGGCCAAAA GGAGCCAATT CAAACAGAAG GGCAGAAGTA TGAACTCCAC GGGTTCTGGA
    AAGAGCAGCG CCACAGTGTC CAGTGTTTCA GAATTGCTGG ACCTGTACGA GGAGGATCCA
    GAAGAGATCT TGTACAATCT TGGATTTGGG CGGGAAGAGC CAGACATTGC CTCTAAAATC
    CCTTCTCGTT TCTTCAACGC GTCGTCTAAC GCTAAAGGCA TCGACATTAA AGTTTACCTG
    GGAGCCCAGA TTCAGCGCAT GGAGATAGAG AACCCAAACT ACGCTCTGAC AAGTCGTTTC
    CGTCAGACCG AAGTCTTGGC TACAGTGGCG AACGTCCTCT CCGATATTTA TTCCCACGTA
    TCTGGAAAAC CCACAGATAA AATCGGCAAT GGTGACATGG AGACCAATGT GCCTAATCCG
    CTAAAAAAGA GAAGCTCTGC TGGAAACTTT GCTAAAATTC TCAAGCGCAA CTTTACGAAG
    CAGAACCTGC TGGCCTCCAT CTCGGATGGA GAAACCAAAC AGCCTAACAA TGAGAGCTCC
    AACATGCGGG ACGTTCAGAC GGATACAGAC CACAAAACAC AACAGAAGAG CTTAAAGAAA
    AAGGATTCAT CTTTGGCGAC CGTGACGGAG GAGGCTCAGC AGAATATCTC TGATGTTTCG
    ATCAGCGCAG ATTTTACGTT GACCAATGGC GAGTCCACAG ACTTGCCTCT GCCTCCGGTC
    CTAGTGAGCA TCAGTCAGGC GTCTGAAGAG AACGATACCT CTGAATTGAA GGTAAACGGG
    TGCCATGAGG CCATCACGTC CACCCCAGAT AAAGAGCCGT CCGGCCTGCT GTCAAACCCA
    CACATCATCC ACTTGCTCGA CCAGCCCAGA GACTCCTTTG AGATGGAGGA GTTGCAGAGT
    AATGAGGATG AAACTCTTCC TGGTCCAGGC GGCATGTCTC GATCTGACAG CGAGCGGTTA
    TTGAGGACAG CCAGCCAGCA GTCTGACAGC AGCGGGTTTG CTGAGGATCC ATCCACAGAC
    GGTTCGGGTA ATCACCTGAA GGTACAAGAG AGCTCAGACA GCTGTGACAG TGAAACCACA
    GTAACATCGC ATGCTGAAGT CAGCACACCC ATAGCATTGG AATACCCCAA GTTTGACAGA
    TTCCCAAGTG AGGAAGTTCA GCTCTACCGA AGCACAACTA CCCAATCTGC AACCAATCAA
    GAAGTCCATG ACATCCCCAA TCTCCAAATT CCCAATCCAA ACTTGGACGA AATTAGCACC
    AATGGACTGC TTCTTTCTCC TGACTCAGAT AGGGCATCTG TCATTGAGTC CGTTTCCACC
    ATAGACTCTC AAGAAGGAAA TTCAAACATG ATGATAGATC ATACAGTGGA CTCCATACTG
    AAAGTAGCTC ATGAGGGCAA CTTATCCCAA CCTAGTCTGG GCATAAATCA AAAGTCTCCT
    GAAGATCTAC TGGAGCAAGA CTCCTCATCA GAGCTTCCAG ACTCATCAGA TCTTAAGCAG
    TCCCACCTTA CAGCTCAAAG GAGATCCATG GCACTCCACA CAGGCCATCA AAGATCATCC
    TCAATGGAGG AGGAGCGTCC TGGCCGAGTG TGGGTGAGGG ATCGGGGCCT GCTGAGGGAG
    AGCCAGGAGA GGAATCCTTT AAGGAGATCC AGCTCCCTCC CATTATCTTC GCTCAACCCG
    GCTCGCGTGG TCACCTCCAT GCGCATCCAG CTTGGAAAAG GTTCAGTCAA GCACTGTACC
    CCGCCATCAT ACACATACCG CTATGAGGAA GACCATTGCA GTGACCGAGA AGAAGTAGAC
    TCCATCTTGG AGGAGGATGA AGTGGATGGT TCGGGTGTCC AATCTGCGCT CCCGAACTCT
    GGATCAAAGA TGAACAGAGG AACTTCACCA CCACCTCCAC CACACCCTCT CGATATACCC
    CAACATCTGG CTCGCTCCTC CACCTCGCTT TATAGCGTTC CCGCTGACTG GCCGCACAGG
    CCTTTGGGTG AAGGTACAAA CTGGAGCGCT AGCGTCCCTA ACCTTTCTCA TTTTACAGCA
    CCATCCCACT TTCCTCATCA CGGAACCTTC TACTCTCCGC AACACGGAGT GTCCTACAAT
    TCCTCATACC CATCTAGGCT TCCCTTCCCA CAGTACCAAA ACTCGCCTTG TCCTCCACCG
    CTTCAAAGCA ACACATTTGT TCCACCACAG GGGGCACACT TTACACCTCA GAACAATATG
    CATTATGCTC ACCCGCACAG TGCTCCCTAC AACCCATTCT ATCCATCTAA TAACATTCCT
    CAAGGAACAC CCTATGACCC ATCGTTGAAT ATGCAAGGCA ACCCGTACCC AAACCATGTC
    CCTCTTGCTC AATCCCACAG CGCCCCTTAC AGTTCGGGAT ACAATGGTTC ACCTCATCAC
    CCTGCTGCTG TTTACAGCCA TCCGTATTAT CCCTCACCCG CCTACCCTTT CTCATTTGGG
    ACGCCTCCTG CCCCTACCCC TGCGATGGGC AGCACAGAGA TGCAGCTCAG GAGGGTCCTG
    CATGACATTC GTGGGACAAT GCAAAATCTT GGTCAGAGCT CATCCGTGCA TGGAGGAGAC
    ACACCCATGG ACAGGATCGG CACACCGCGC TCCGTCCAAC CGCTTTACGA GGAGCTCCAG
    ATGCGGCGCA GGAGTCTGAA TGTCTTCCGG ACTCAGATGA TGGATCTTGA ACTGGCCCTC
    ATGAGACAGC AGTCTTTGGT CTACCAGCAT CTCAGTCCAG AAGAGAGGCG GGAGGCAGAG
    CAGCTTCAGA CGCTGCGGAC TGCAGTCAGG CAGGAGCTGC AGGAGCTGGA GCTGCAATTG
    GAGGATCGGC TGCTCTCTAT CAATGAACAA ATGCGCAACA GCTCGCAACA CATCGGCTTC
    TACCGACACC ACATGGGCAT GCACAGAGGT CACAGCATGG AAAGCCTGTC TAATTCTTCA
    GCACTGAGGG CCATGGAGCC GGTGACGGAT CTTCTGAAGG AGCAATTGTA CCTTCAGTCT
    GAGCTGGCAT ATGAGGCTGC AACCCCCAGT TCAGGCCGTT CATCGAGGGC GTCCAGTCCT
    GTCAGATTCA ACCGAACACT GGAGTCTTGC TCTCCATCCC CACAGAGAGC CAGCGTTTAC
    CGGGCCACCC TCAACCTCAT GCCCACCCTA CCTCCACGGC CGGGGATGGA AGCTCCTCGG
    GATGTTTCTC CTGAGAGGAT GGAGTCAAAC CCGACACCCA CTTCTCTGGA GGTCAGCAGA
    GATGAGGACG AGGTGTCACT GGATGGTCAT GGGAGCAGGA CGGCCGAAGA TTGCAGTCCC
    ACAGAGTGGA GGAGTAGAAG TAGAGGAAAT GGAGACCTGC AGCATCTCAT AAAGGAGATT
    AAAGAAAGCA TCGCACATGA AATCCGGCAG GAGATCGTGA ATGAGCTTCT GGCTGCCGTG
    TCGCCACACA GATCTCCTCT TCCAGCAAGG CAGCCCCCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213332.1
    CDS151..3975
    Translation

    Target ORF information:

    RefSeq Version XM_017357843.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio sperm specific antigen 2 (ssfa2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357843.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAGG TTGAATCGCC CCCTGCCAGA TTCAAGAGGA AGGCCTGGGC TCAGAGCCGA 
    GACTCCTGGC AGGCGTCTGA GTCTCAGGAC CAGGGCGCAA AAGAGCCGCT GTCTGCCCAG
    CAGCCTATGG AGGAGAAGAC TTCCACAGCC TCAGAAGCAG GTCGTAATCC AAATCAAGTG
    GAGAACTGGC TCAGGACCTG CAGGACTCCT CTCGGGGCCT CTTTGGATGA CCAGAGCAGC
    AGTCCGTCCA GAGGGCCGCT GAAGAATGGC TGTAGTTTTG AAGATGATCT GTCACTGGGA
    GCTGAGGCTA ATCACCTCCA GAACAGAAAA ACAGAGGCCG ATGGCTATGA AATGCACGCC
    AAGGCCAAAA GGAGCCAATT CAAACAGAAG GGCAGAAGTA TGAACTCCAC GGGTTCTGGA
    AAGAGCAGCG CCACAGTGTC CAGTGTTTCA GAATTGCTGG ACCTGTACGA GGAGGATCCA
    GAAGAGATCT TGTACAATCT TGGATTTGGG CGGGAAGAGC CAGACATTGC CTCTAAAATC
    CCTTCTCGTT TCTTCAACGC GTCGTCTAAC GCTAAAGGCA TCGACATTAA AGTTTACCTG
    GGAGCCCAGA TTCAGCGCAT GGAGATAGAG AACCCAAACT ACGCTCTGAC AAGTCGTTTC
    CGTCAGACCG AAGTCTTGGC TACAGTGGCG AACGTCCTCT CCGATATTTA TTCCCACGTA
    TCTGGAAAAC CCACAGATAA AATCGGCAAT GGTGACATGG AGACCAATGT GCCTAATCCG
    CTAAAAAAGA GAAGCTCTGC TGGAAACTTT GCTAAAATTC TCAAGCGCAA CTTTACGAAG
    CAGAACCTGC TGGCCTCCAT CTCGGATGGA GAAACCAAAC AGCCTAACAA TGAGAGCTCC
    AACATGCGGG ACGTTCAGAC GGATACAGAC CACAAAACAC AACAGAAGAG CTTAAAGAAA
    AAGGATTCAT CTTTGGCGAC CGTGACGGAG GAGGCTCAGC AGAATATCTC TGATGTTTCG
    ATCAGCGCAG ATTTTACGTT GACCAATGGC GAGTCCACAG ACTTGCCTCT GCCTCCGGTC
    CTAGTGAGCA TCAGTCAGGC GTCTGAAGAG AACGATACCT CTGAATTGAA GGTAAACGGG
    TGCCATGAGG CCATCACGTC CACCCCAGAT AAAGAGCCGT CCGGCCTGCT GTCAAACCCA
    CACATCATCC ACTTGCTCGA CCAGCCCAGA GACTCCTTTG AGATGGAGGA GTTGCAGAGT
    AATGAGGATG AAACTCTTCC TGGTCCAGGC GGCATGTCTC GATCTGACAG CGAGCGGTTA
    TTGAGGACAG CCAGCCAGCA GTCTGACAGC AGCGGGTTTG CTGAGGATCC ATCCACAGAC
    GGTTCGGGTA ATCACCTGAA GGTACAAGAG AGCTCAGACA GCTGTGACAG TGAAACCACA
    GTAACATCGC ATGCTGAAGT CAGCACACCC ATAGCATTGG AATACCCCAA GTTTGACAGA
    TTCCCAAGTG AGGAAGTTCA GCTCTACCGA AGCACAACTA CCCAATCTGC AACCAATCAA
    GAAGTCCATG ACATCCCCAA TCTCCAAATT CCCAATCCAA ACTTGGACGA AATTAGCACC
    AATGGACTGC TTCTTTCTCC TGACTCAGAT AGGGCATCTG TCATTGAGTC CGTTTCCACC
    ATAGACTCTC AAGAAGGAAA TTCAAACATG ATGATAGATC ATACAGTGGA CTCCATACTG
    AAAGTAGCTC ATGAGGGCAA CTTATCCCAA CCTAGTCTGG GCATAAATCA AAAGTCTCCT
    GAAGATCTAC TGGAGCAAGA CTCCTCATCA GAGCTTCCAG ACTCATCAGA TCTTAAGCAG
    TCCCACCTTA CAGCTCAAAG GAGATCCATG GCACTCCACA CAGGCCATCA AAGATCATCC
    TCAATGGAGG AGGAGCGTCC TGGCCGAGTG TGGGTGAGGG ATCGGGGCCT GCTGAGGGAG
    AGCCAGGAGA GGAATCCTTT AAGGAGATCC AGCTCCCTCC CATTATCTTC GCTCAACCCG
    GCTCGCGTGG TCACCTCCAT GCGCATCCAG CTTGGAAAAG GTTCAGTCAA GCACTGTACC
    CCGCCATCAT ACACATACCG CTATGAGGAA GACCATTGCA GTGACCGAGA AGAAGTAGAC
    TCCATCTTGG AGGAGGATGA AGTGGATGGT TCGGGTGTCC AATCTGCGCT CCCGAACTCT
    GGATCAAAGA TGAACAGAGG AACTTCACCA CCACCTCCAC CACACCCTCT CGATATACCC
    CAACATCTGG CTCGCTCCTC CACCTCGCTT TATAGCGTTC CCGCTGACTG GCCGCACAGG
    CCTTTGGGTG AAGGTACAAA CTGGAGCGCT AGCGTCCCTA ACCTTTCTCA TTTTACAGCA
    CCATCCCACT TTCCTCATCA CGGAACCTTC TACTCTCCGC AACACGGAGT GTCCTACAAT
    TCCTCATACC CATCTAGGCT TCCCTTCCCA CAGTACCAAA ACTCGCCTTG TCCTCCACCG
    CTTCAAAGCA ACACATTTGT TCCACCACAG GGGGCACACT TTACACCTCA GAACAATATG
    CATTATGCTC ACCCGCACAG TGCTCCCTAC AACCCATTCT ATCCATCTAA TAACATTCCT
    CAAGGAACAC CCTATGACCC ATCGTTGAAT ATGCAAGGCA ACCCGTACCC AAACCATGTC
    CCTCTTGCTC AATCCCACAG CGCCCCTTAC AGTTCGGGAT ACAATGGTTC ACCTCATCAC
    CCTGCTGCTG TTTACAGCCA TCCGTATTAT CCCTCACCCG CCTACCCTTT CTCATTTGGG
    ACGCCTCCTG CCCCTACCCC TGCGATGGGC AGCACAGAGA TGCAGCTCAG GAGGGTCCTG
    CATGACATTC GTGGGACAAT GCAAAATCTT GGTCAGAGCT CATCCGTGCA TGGAGGAGAC
    ACACCCATGG ACAGGATCGG CACACCGCGC TCCGTCCAAC CGCTTTACGA GGAGCTCCAG
    ATGCGGCGCA GGAGTCTGAA TGTCTTCCGG ACTCAGATGA TGGATCTTGA ACTGGCCCTC
    ATGAGACAGC AGTCTTTGGT CTACCAGCAT CTCAGTCCAG AAGAGAGGCG GGAGGCAGAG
    CAGCTTCAGA CGCTGCGGAC TGCAGTCAGG CAGGAGCTGC AGGAGCTGGA GCTGCAATTG
    GAGGATCGGC TGCTCTCTAT CAATGAACAA ATGCGCAACA GCTCGCAACA CATCGGCTTC
    TACCGACACC ACATGGGCAT GCACAGAGGT CACAGCATGG AAAGCCTGTC TAATTCTTCA
    GCACTGAGGG CCATGGAGCC GGTGACGGAT CTTCTGAAGG AGCAATTGTA CCTTCAGTCT
    GAGCTGGCAT ATGAGGCTGC AACCCCCAGT TCAGGCCGTT CATCGAGGGC GTCCAGTCCT
    GTCAGATTCA ACCGAACACT GGAGTCTTGC TCTCCATCCC CACAGAGAGC CAGCGTTTAC
    CGGGCCACCC TCAACCTCAT GCCCACCCTA CCTCCACGGC CGGGGATGGA AGCTCCTCGG
    GATGTTTCTC CTGAGAGGAT GGAGTCAAAC CCGACACCCA CTTCTCTGGA GGTCAGCAGA
    GATGAGGACG AGGTGTCACT GGATGGTCAT GGGAGCAGGA CGGCCGAAGA TTGCAGTCCC
    ACAGAGTGGA GGAGTAGAAG TAGAGGAAAT GGAGACCTGC AGCATCTCAT AAAGGAGATT
    AAAGAAAGCA TCGCACATGA AATCCGGCAG GAGATCGTGA ATGAGCTTCT GGCTGCCGTG
    TCGCCACACA GATCTCCTCT TCCAGCAAGG CAGCCCCCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60372
    Clone ID Related Accession (Same CDS sequence) XM_017357843.2 , XM_017357843.1 , XM_021479016.1
    Accession Version XM_021479016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product sperm-specific antigen 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) and transcript sequence (GDQQ01039019.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 209 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAGG TTGAATCGCC CCCTGCCAGA TTCAAGAGGA AGGCCTGGGC TCAGAGCCGA 
    GACTCCTGGC AGGCGTCTGA GTCTCAGGAC CAGGGCGCAA AAGAGCCGCT GTCTGCCCAG
    CAGCCTATGG AGGAGAAGAC TTCCACAGCC TCAGAAGCAG GTCGTAATCC AAATCAAGTG
    GAGAACTGGC TCAGGACCTG CAGGACTCCT CTCGGGGCCT CTTTGGATGA CCAGAGCAGC
    AGTCCGTCCA GAGGGCCGCT GAAGAATGGC TGTAGTTTTG AAGATGATCT GTCACTGGGA
    GCTGAGGCTA ATCACCTCCA GAACAGAAAA ACAGAGGCCG ATGGCTATGA AATGCACGCC
    AAGGCCAAAA GGAGCCAATT CAAACAGAAG GGCAGAAGTA TGAACTCCAC GGGTTCTGGA
    AAGAGCAGCG CCACAGTGTC CAGTGTTTCA GAATTGCTGG ACCTGTACGA GGAGGATCCA
    GAAGAGATCT TGTACAATCT TGGATTTGGG CGGGAAGAGC CAGACATTGC CTCTAAAATC
    CCTTCTCGTT TCTTCAACGC GTCGTCTAAC GCTAAAGGCA TCGACATTAA AGTTTACCTG
    GGAGCCCAGA TTCAGCGCAT GGAGATAGAG AACCCAAACT ACGCTCTGAC AAGTCGTTTC
    CGTCAGACCG AAGTCTTGGC TACAGTGGCG AACGTCCTCT CCGATATTTA TTCCCACGTA
    TCTGGAAAAC CCACAGATAA AATCGGCAAT GGTGACATGG AGACCAATGT GCCTAATCCG
    CTAAAAAAGA GAAGCTCTGC TGGAAACTTT GCTAAAATTC TCAAGCGCAA CTTTACGAAG
    CAGAACCTGC TGGCCTCCAT CTCGGATGGA GAAACCAAAC AGCCTAACAA TGAGAGCTCC
    AACATGCGGG ACGTTCAGAC GGATACAGAC CACAAAACAC AACAGAAGAG CTTAAAGAAA
    AAGGATTCAT CTTTGGCGAC CGTGACGGAG GAGGCTCAGC AGAATATCTC TGATGTTTCG
    ATCAGCGCAG ATTTTACGTT GACCAATGGC GAGTCCACAG ACTTGCCTCT GCCTCCGGTC
    CTAGTGAGCA TCAGTCAGGC GTCTGAAGAG AACGATACCT CTGAATTGAA GGTAAACGGG
    TGCCATGAGG CCATCACGTC CACCCCAGAT AAAGAGCCGT CCGGCCTGCT GTCAAACCCA
    CACATCATCC ACTTGCTCGA CCAGCCCAGA GACTCCTTTG AGATGGAGGA GTTGCAGAGT
    AATGAGGATG AAACTCTTCC TGGTCCAGGC GGCATGTCTC GATCTGACAG CGAGCGGTTA
    TTGAGGACAG CCAGCCAGCA GTCTGACAGC AGCGGGTTTG CTGAGGATCC ATCCACAGAC
    GGTTCGGGTA ATCACCTGAA GGTACAAGAG AGCTCAGACA GCTGTGACAG TGAAACCACA
    GTAACATCGC ATGCTGAAGT CAGCACACCC ATAGCATTGG AATACCCCAA GTTTGACAGA
    TTCCCAAGTG AGGAAGTTCA GCTCTACCGA AGCACAACTA CCCAATCTGC AACCAATCAA
    GAAGTCCATG ACATCCCCAA TCTCCAAATT CCCAATCCAA ACTTGGACGA AATTAGCACC
    AATGGACTGC TTCTTTCTCC TGACTCAGAT AGGGCATCTG TCATTGAGTC CGTTTCCACC
    ATAGACTCTC AAGAAGGAAA TTCAAACATG ATGATAGATC ATACAGTGGA CTCCATACTG
    AAAGTAGCTC ATGAGGGCAA CTTATCCCAA CCTAGTCTGG GCATAAATCA AAAGTCTCCT
    GAAGATCTAC TGGAGCAAGA CTCCTCATCA GAGCTTCCAG ACTCATCAGA TCTTAAGCAG
    TCCCACCTTA CAGCTCAAAG GAGATCCATG GCACTCCACA CAGGCCATCA AAGATCATCC
    TCAATGGAGG AGGAGCGTCC TGGCCGAGTG TGGGTGAGGG ATCGGGGCCT GCTGAGGGAG
    AGCCAGGAGA GGAATCCTTT AAGGAGATCC AGCTCCCTCC CATTATCTTC GCTCAACCCG
    GCTCGCGTGG TCACCTCCAT GCGCATCCAG CTTGGAAAAG GTTCAGTCAA GCACTGTACC
    CCGCCATCAT ACACATACCG CTATGAGGAA GACCATTGCA GTGACCGAGA AGAAGTAGAC
    TCCATCTTGG AGGAGGATGA AGTGGATGGT TCGGGTGTCC AATCTGCGCT CCCGAACTCT
    GGATCAAAGA TGAACAGAGG AACTTCACCA CCACCTCCAC CACACCCTCT CGATATACCC
    CAACATCTGG CTCGCTCCTC CACCTCGCTT TATAGCGTTC CCGCTGACTG GCCGCACAGG
    CCTTTGGGTG AAGGTACAAA CTGGAGCGCT AGCGTCCCTA ACCTTTCTCA TTTTACAGCA
    CCATCCCACT TTCCTCATCA CGGAACCTTC TACTCTCCGC AACACGGAGT GTCCTACAAT
    TCCTCATACC CATCTAGGCT TCCCTTCCCA CAGTACCAAA ACTCGCCTTG TCCTCCACCG
    CTTCAAAGCA ACACATTTGT TCCACCACAG GGGGCACACT TTACACCTCA GAACAATATG
    CATTATGCTC ACCCGCACAG TGCTCCCTAC AACCCATTCT ATCCATCTAA TAACATTCCT
    CAAGGAACAC CCTATGACCC ATCGTTGAAT ATGCAAGGCA ACCCGTACCC AAACCATGTC
    CCTCTTGCTC AATCCCACAG CGCCCCTTAC AGTTCGGGAT ACAATGGTTC ACCTCATCAC
    CCTGCTGCTG TTTACAGCCA TCCGTATTAT CCCTCACCCG CCTACCCTTT CTCATTTGGG
    ACGCCTCCTG CCCCTACCCC TGCGATGGGC AGCACAGAGA TGCAGCTCAG GAGGGTCCTG
    CATGACATTC GTGGGACAAT GCAAAATCTT GGTCAGAGCT CATCCGTGCA TGGAGGAGAC
    ACACCCATGG ACAGGATCGG CACACCGCGC TCCGTCCAAC CGCTTTACGA GGAGCTCCAG
    ATGCGGCGCA GGAGTCTGAA TGTCTTCCGG ACTCAGATGA TGGATCTTGA ACTGGCCCTC
    ATGAGACAGC AGTCTTTGGT CTACCAGCAT CTCAGTCCAG AAGAGAGGCG GGAGGCAGAG
    CAGCTTCAGA CGCTGCGGAC TGCAGTCAGG CAGGAGCTGC AGGAGCTGGA GCTGCAATTG
    GAGGATCGGC TGCTCTCTAT CAATGAACAA ATGCGCAACA GCTCGCAACA CATCGGCTTC
    TACCGACACC ACATGGGCAT GCACAGAGGT CACAGCATGG AAAGCCTGTC TAATTCTTCA
    GCACTGAGGG CCATGGAGCC GGTGACGGAT CTTCTGAAGG AGCAATTGTA CCTTCAGTCT
    GAGCTGGCAT ATGAGGCTGC AACCCCCAGT TCAGGCCGTT CATCGAGGGC GTCCAGTCCT
    GTCAGATTCA ACCGAACACT GGAGTCTTGC TCTCCATCCC CACAGAGAGC CAGCGTTTAC
    CGGGCCACCC TCAACCTCAT GCCCACCCTA CCTCCACGGC CGGGGATGGA AGCTCCTCGG
    GATGTTTCTC CTGAGAGGAT GGAGTCAAAC CCGACACCCA CTTCTCTGGA GGTCAGCAGA
    GATGAGGACG AGGTGTCACT GGATGGTCAT GGGAGCAGGA CGGCCGAAGA TTGCAGTCCC
    ACAGAGTGGA GGAGTAGAAG TAGAGGAAAT GGAGACCTGC AGCATCTCAT AAAGGAGATT
    AAAGAAAGCA TCGCACATGA AATCCGGCAG GAGATCGTGA ATGAGCTTCT GGCTGCCGTG
    TCGCCACACA GATCTCCTCT TCCAGCAAGG CAGCCCCCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334691.1
    CDS161..3985
    Translation

    Target ORF information:

    RefSeq Version XM_021479016.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio sperm specific antigen 2 (ssfa2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAGG TTGAATCGCC CCCTGCCAGA TTCAAGAGGA AGGCCTGGGC TCAGAGCCGA 
    GACTCCTGGC AGGCGTCTGA GTCTCAGGAC CAGGGCGCAA AAGAGCCGCT GTCTGCCCAG
    CAGCCTATGG AGGAGAAGAC TTCCACAGCC TCAGAAGCAG GTCGTAATCC AAATCAAGTG
    GAGAACTGGC TCAGGACCTG CAGGACTCCT CTCGGGGCCT CTTTGGATGA CCAGAGCAGC
    AGTCCGTCCA GAGGGCCGCT GAAGAATGGC TGTAGTTTTG AAGATGATCT GTCACTGGGA
    GCTGAGGCTA ATCACCTCCA GAACAGAAAA ACAGAGGCCG ATGGCTATGA AATGCACGCC
    AAGGCCAAAA GGAGCCAATT CAAACAGAAG GGCAGAAGTA TGAACTCCAC GGGTTCTGGA
    AAGAGCAGCG CCACAGTGTC CAGTGTTTCA GAATTGCTGG ACCTGTACGA GGAGGATCCA
    GAAGAGATCT TGTACAATCT TGGATTTGGG CGGGAAGAGC CAGACATTGC CTCTAAAATC
    CCTTCTCGTT TCTTCAACGC GTCGTCTAAC GCTAAAGGCA TCGACATTAA AGTTTACCTG
    GGAGCCCAGA TTCAGCGCAT GGAGATAGAG AACCCAAACT ACGCTCTGAC AAGTCGTTTC
    CGTCAGACCG AAGTCTTGGC TACAGTGGCG AACGTCCTCT CCGATATTTA TTCCCACGTA
    TCTGGAAAAC CCACAGATAA AATCGGCAAT GGTGACATGG AGACCAATGT GCCTAATCCG
    CTAAAAAAGA GAAGCTCTGC TGGAAACTTT GCTAAAATTC TCAAGCGCAA CTTTACGAAG
    CAGAACCTGC TGGCCTCCAT CTCGGATGGA GAAACCAAAC AGCCTAACAA TGAGAGCTCC
    AACATGCGGG ACGTTCAGAC GGATACAGAC CACAAAACAC AACAGAAGAG CTTAAAGAAA
    AAGGATTCAT CTTTGGCGAC CGTGACGGAG GAGGCTCAGC AGAATATCTC TGATGTTTCG
    ATCAGCGCAG ATTTTACGTT GACCAATGGC GAGTCCACAG ACTTGCCTCT GCCTCCGGTC
    CTAGTGAGCA TCAGTCAGGC GTCTGAAGAG AACGATACCT CTGAATTGAA GGTAAACGGG
    TGCCATGAGG CCATCACGTC CACCCCAGAT AAAGAGCCGT CCGGCCTGCT GTCAAACCCA
    CACATCATCC ACTTGCTCGA CCAGCCCAGA GACTCCTTTG AGATGGAGGA GTTGCAGAGT
    AATGAGGATG AAACTCTTCC TGGTCCAGGC GGCATGTCTC GATCTGACAG CGAGCGGTTA
    TTGAGGACAG CCAGCCAGCA GTCTGACAGC AGCGGGTTTG CTGAGGATCC ATCCACAGAC
    GGTTCGGGTA ATCACCTGAA GGTACAAGAG AGCTCAGACA GCTGTGACAG TGAAACCACA
    GTAACATCGC ATGCTGAAGT CAGCACACCC ATAGCATTGG AATACCCCAA GTTTGACAGA
    TTCCCAAGTG AGGAAGTTCA GCTCTACCGA AGCACAACTA CCCAATCTGC AACCAATCAA
    GAAGTCCATG ACATCCCCAA TCTCCAAATT CCCAATCCAA ACTTGGACGA AATTAGCACC
    AATGGACTGC TTCTTTCTCC TGACTCAGAT AGGGCATCTG TCATTGAGTC CGTTTCCACC
    ATAGACTCTC AAGAAGGAAA TTCAAACATG ATGATAGATC ATACAGTGGA CTCCATACTG
    AAAGTAGCTC ATGAGGGCAA CTTATCCCAA CCTAGTCTGG GCATAAATCA AAAGTCTCCT
    GAAGATCTAC TGGAGCAAGA CTCCTCATCA GAGCTTCCAG ACTCATCAGA TCTTAAGCAG
    TCCCACCTTA CAGCTCAAAG GAGATCCATG GCACTCCACA CAGGCCATCA AAGATCATCC
    TCAATGGAGG AGGAGCGTCC TGGCCGAGTG TGGGTGAGGG ATCGGGGCCT GCTGAGGGAG
    AGCCAGGAGA GGAATCCTTT AAGGAGATCC AGCTCCCTCC CATTATCTTC GCTCAACCCG
    GCTCGCGTGG TCACCTCCAT GCGCATCCAG CTTGGAAAAG GTTCAGTCAA GCACTGTACC
    CCGCCATCAT ACACATACCG CTATGAGGAA GACCATTGCA GTGACCGAGA AGAAGTAGAC
    TCCATCTTGG AGGAGGATGA AGTGGATGGT TCGGGTGTCC AATCTGCGCT CCCGAACTCT
    GGATCAAAGA TGAACAGAGG AACTTCACCA CCACCTCCAC CACACCCTCT CGATATACCC
    CAACATCTGG CTCGCTCCTC CACCTCGCTT TATAGCGTTC CCGCTGACTG GCCGCACAGG
    CCTTTGGGTG AAGGTACAAA CTGGAGCGCT AGCGTCCCTA ACCTTTCTCA TTTTACAGCA
    CCATCCCACT TTCCTCATCA CGGAACCTTC TACTCTCCGC AACACGGAGT GTCCTACAAT
    TCCTCATACC CATCTAGGCT TCCCTTCCCA CAGTACCAAA ACTCGCCTTG TCCTCCACCG
    CTTCAAAGCA ACACATTTGT TCCACCACAG GGGGCACACT TTACACCTCA GAACAATATG
    CATTATGCTC ACCCGCACAG TGCTCCCTAC AACCCATTCT ATCCATCTAA TAACATTCCT
    CAAGGAACAC CCTATGACCC ATCGTTGAAT ATGCAAGGCA ACCCGTACCC AAACCATGTC
    CCTCTTGCTC AATCCCACAG CGCCCCTTAC AGTTCGGGAT ACAATGGTTC ACCTCATCAC
    CCTGCTGCTG TTTACAGCCA TCCGTATTAT CCCTCACCCG CCTACCCTTT CTCATTTGGG
    ACGCCTCCTG CCCCTACCCC TGCGATGGGC AGCACAGAGA TGCAGCTCAG GAGGGTCCTG
    CATGACATTC GTGGGACAAT GCAAAATCTT GGTCAGAGCT CATCCGTGCA TGGAGGAGAC
    ACACCCATGG ACAGGATCGG CACACCGCGC TCCGTCCAAC CGCTTTACGA GGAGCTCCAG
    ATGCGGCGCA GGAGTCTGAA TGTCTTCCGG ACTCAGATGA TGGATCTTGA ACTGGCCCTC
    ATGAGACAGC AGTCTTTGGT CTACCAGCAT CTCAGTCCAG AAGAGAGGCG GGAGGCAGAG
    CAGCTTCAGA CGCTGCGGAC TGCAGTCAGG CAGGAGCTGC AGGAGCTGGA GCTGCAATTG
    GAGGATCGGC TGCTCTCTAT CAATGAACAA ATGCGCAACA GCTCGCAACA CATCGGCTTC
    TACCGACACC ACATGGGCAT GCACAGAGGT CACAGCATGG AAAGCCTGTC TAATTCTTCA
    GCACTGAGGG CCATGGAGCC GGTGACGGAT CTTCTGAAGG AGCAATTGTA CCTTCAGTCT
    GAGCTGGCAT ATGAGGCTGC AACCCCCAGT TCAGGCCGTT CATCGAGGGC GTCCAGTCCT
    GTCAGATTCA ACCGAACACT GGAGTCTTGC TCTCCATCCC CACAGAGAGC CAGCGTTTAC
    CGGGCCACCC TCAACCTCAT GCCCACCCTA CCTCCACGGC CGGGGATGGA AGCTCCTCGG
    GATGTTTCTC CTGAGAGGAT GGAGTCAAAC CCGACACCCA CTTCTCTGGA GGTCAGCAGA
    GATGAGGACG AGGTGTCACT GGATGGTCAT GGGAGCAGGA CGGCCGAAGA TTGCAGTCCC
    ACAGAGTGGA GGAGTAGAAG TAGAGGAAAT GGAGACCTGC AGCATCTCAT AAAGGAGATT
    AAAGAAAGCA TCGCACATGA AATCCGGCAG GAGATCGTGA ATGAGCTTCT GGCTGCCGTG
    TCGCCACACA GATCTCCTCT TCCAGCAAGG CAGCCCCCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.