vcla cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vcla
Entrez Gene ID	552980
Full Name	vinculin a
Synonyms	hm:zehn2160,im:6902790,vcl
General protein information	Preferred Names vinculin a Names vinculin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005156840.3	XP_005156897.1	vinculin isoform X1
NM_001128681.1	NP_001122153.1	vinculin
XM_005156840.4	XP_005156897.1	vinculin isoform X1
XM_005156842.3	XP_005156899.1	vinculin isoform X2
XM_021480576.1	XP_021336251.1	vinculin isoform X2
XM_005156842.4	XP_005156899.1	vinculin isoform X3

Pathways from BioSystems

Homologies

	VCL	XP_001118118.1
Gallus gallus (chicken)	VCL	NP_990772.1
Xenopus tropicalis (tropical clawed frog)	vcl	NP_001090722.1
Bos taurus (cattle)	VCL	NP_001178299.1
Canis lupus familiaris (dog)	VCL	XP_536395.3
Danio rerio (zebrafish)	vcla	NP_001122153.1
Homo sapiens (human)	VCL	NP_054706.1
Pan troglodytes (chimpanzee)	VCL	XP_507854.2
Mus musculus (house mouse)	Vcl	NP_033528.3
Rattus norvegicus (Norway rat)	Vcl	NP_001100718.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Zygotic vinculin is not essential for embryonic development in zebrafish.
Han MKL, van der Krogt GNM, de Rooij J
PloS one12(8)e0182278(2017)

Identification and Expression Analysis of the Complete Family of Zebrafish
England SJ, Campbell PC, Banerjee S, Swanson AJ, Lewis KE
Frontiers in cell and developmental biology55(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study show that zebrafish contain two homologous vinculin isoforms, of which the sequence of key functional domains is conserved with several higher vertebrate. Loss of both zygotic vcla and maternal contribution of vcla did not result in notable defects, while the additional loss of zygotic vclb is lethal only after embryonic stages. These data indicate that embryonic development in zebrafish can occur without vinculin.
Title: Zygotic vinculin is not essential for embryonic development in zebrafish.

The following vcla gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vcla cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa18784	XM_021480576.1 Latest version!	Danio rerio vinculin a (vcla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa03921	NM_001128681.1 Latest version!	Danio rerio vinculin a (vcla), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa18783	XM_005156840.4 Latest version!	Danio rerio vinculin a (vcla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa18785	XM_005156842.4 Latest version!	Danio rerio vinculin a (vcla), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa18783	XM_005156840.3	Danio rerio vinculin a (vcla), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa18785	XM_005156842.3	Danio rerio vinculin a (vcla), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vcla ( XM_021480576.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336251.1 Danio rerio vinculin a (vcla), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18784
Clone ID Related Accession (Same CDS sequence)	XM_021480576.1
Accession Version	XM_021480576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAAAGGACA CTGAGGCCAT GAAGAGAGCG CTGGCGCTGA TCGAGTCTAA GATGGGTCAG 
GCTAAAGGCT GGCTGAGGGA CCCCAATGCA CTGCCAGGAG ATCCAGGAGA GCATGCGCTG 
CGTCAGATCC TGGATGAAGC AGGAAAAGTG GGTGAGCTTT GTGCAGGGAA AGAGAGACGA 
GAGATCCTGG GAACAGCCAA AACCCTGGGC CAGATGACGG ATCAGGTGTC AGATGTGCGC 
GCCAGAGGTC AGGGTGCCAC ACCTATGGGT ATGCAGAAAG CTCAGCAGGT TGCTCAAGGT 
TTGGATATTC TGGTGGGAAA AGTGGAGAAC GCAGCCCGTA AGCTGGAGGC CCTAACCAAT 
GCCAAACAGG CCATCGCCAA GAGAATCGAC AATGCCCAGA GCTGGCTGGC TGATCCTAAC 
GGCGGTCCAG AGGGTGAAGA AAACATCCGC GCTCTTCTGG CGGAAGCCAA ACGCATCGCT 
GACTTGTGTG AAGACCCTAA AGAACGAGAT GACATTCTTC GTTCCATCGG CGAGATCGCA 
GGCCTCACCG CCAGGCTGGT CGAGCTCCGT AGAATAGGTA AAGGCGATAC TCCAGAGGCG 
AGAGCTCTTG CCAAACAGAT TGGCACAGCT TTGCAGAACT TACAGGCAAA AACGAACCGT 
GCTGTGGCCA ACATGAGGCC TGCCAAAGCT GCTGTTACCC TGGAGGGCAA AATGGAGCAG 
GCCTTGCGCT GGATCAACAA CCCTGGAGTG GACGATCATG GAGTAGGCCA GGCTGCTATA 
CGAGGGCTGA TAGCAGAGGG TCGACGGCTC GCGAGCTCTC TGCCAGGGCC GTACAGACAG 
GAGCTGCTGG CCAAATGTGA GCAGGTGGAG CAGCTGATGA TGCAGCTGGC GGATCTGGCA 
GCGCGGGGAG AAGGAGAGAG TCCTCAGGCC CGCGCAGTGG CTGCTCATCT TCTGGAGGCA 
ATTAAAGATC TGAAGGCAAA GATGCAGGAA GCAATGACCC AGGAAGTGTC TGATGTTTTC 
AGCGATACAA CAACACCAAT TAAACTCCTG GCTGTGGCAG CAACAGCACC TCTCGAGGCC 
CCGAACAGAG AGGAGGTGTT TGAAGAAAGG GCTTCTAACT TTGAGAATCA TGCTAGCAGG 
CTGGGTGCTA CTGCGGAGAA GGCGGCTGCG GTAGGAACAG CCAATAAAAG CACAGTTGAA 
GGCATCCAAG CGGCTGTGAA ATCAGCTAGA GATCTAACAC CTCAGGTCAC TTCCGCTGCA 
CGTATTTTGC TGAAGAACCC TGGCAACCAG GCCGCCTACG AGCACTTTGA TACCATGAAG 
AACCAGTGGA TTGACAACAT TGAAAAAATG ACCAGTCTTG TGGATGAAGC CATTGACACT 
AAATCCCTCT TGGATGCTTC AGAGGAAGCT ATTAAGAAAG ACATTGATAA ATGCCGTGTA 
GCAATGGCTA ACGTTCAGCC CCAGATGTTG GTTGCAGGGG CTACAAGCAT TGCCCGGCGG 
GCAAACAGAG TACTTCTGGT GGCCAAGAGG GAAGTGGAGA ATTCAGAGGA CCCTAAATTC 
AGAGAACTGG TTAAAGCAGC TTCAGATGAA CTCGGTAGAA CCATCTCACC CATGGTTATG 
GCTGCTAAAG GAGTGGCAGG AAACATCCAG GATCCAGGTT TGCAAAAGGG CTTCCTGGAC 
TCTGGCTACA GGATCTTGGC TGCTGTTGGG AAAGTCAGAG AAGCCTTTCA GCCTCAGGAG 
CTGGACTTTC CTCCGCCACC TCCAGACCTG GATCAGCTGC ATGTCAATGA TGACCAGGCT 
CCACCCAAAC CACCTCTTCC TGAGGGAGAG GTTCCTCCGC CGAGACCTCC ACCTCCAGAA 
GAGAAAGATG AGGAGTTCCC TGAGCAGAAG GCAGGAGAGA TGGTGAGCGA GCCCATGATG 
GTGGCTGCCA GGTCACTGCA CGACGAGGCC CGCAAGTGGT CCAGCAAGGG TAATGACATC 
ATTGGAGCCG CCAAGCGTAT GGCCCTGCTG ATGGCAGAGA TGTCTCGTCT GGTGCGTGGT 
GGAAGTGGGA ATAAACGTGC CCTTATTCAG TGCGCCAAGG ACATCGCCAA GGCCTCTGAT 
GAGGTCACGC GGCTGGCTAA AGAAGTAGCC AAACAATGTA CTGACAAAAG GATTCGCACA 
AACCTGCTGC AGGTTTGTGA GCGCATCCCA ACCATCAGCA CTCAGTTGAA GATCCTTTCC 
ACAGTGAAGG CCACTATGCT GGGACGTACA AATATCAGTG AGGAAGAATC CGAACAGGCC 
ACTGAGATGC TTGTGCACAA CGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACGGTGAGA 
GAAGCAGAAG CCGCTTCCAT CAAGATCCGC ACAGACGCAG GCTTCACACT TCGCTGGGTC 
CGAAAAACCC CCTGGTACCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336251.1
CDS	217..3420
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTSPVAAVQAAVSNLVRVGKETVQTTEDKIMKRDMPSAFIKVENACAKLVEAAQMLRTDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLITYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVTSMNTVKELLPVLISAIKIFVTTKCTKSHGVEEALKNRNYTFDKMTAEINEIIRVLQLTSWDEDAWANKKDTEAMKRALALIESKMGQAKGWLRDPNALPGDPGEHALRQILDEAGKVGELCAGKERREILGTAKTLGQMTDQVSDVRARGQGATPMGMQKAQQVAQGLDILVGKVENAARKLEALTNAKQAIAKRIDNAQSWLADPNGGPEGEENIRALLAEAKRIADLCEDPKERDDILRSIGEIAGLTARLVELRRIGKGDTPEARALAKQIGTALQNLQAKTNRAVANMRPAKAAVTLEGKMEQALRWINNPGVDDHGVGQAAIRGLIAEGRRLASSLPGPYRQELLAKCEQVEQLMMQLADLAARGEGESPQARAVAAHLLEAIKDLKAKMQEAMTQEVSDVFSDTTTPIKLLAVAATAPLEAPNREEVFEERASNFENHASRLGATAEKAAAVGTANKSTVEGIQAAVKSARDLTPQVTSAARILLKNPGNQAAYEHFDTMKNQWIDNIEKMTSLVDEAIDTKSLLDASEEAIKKDIDKCRVAMANVQPQMLVAGATSIARRANRVLLVAKREVENSEDPKFRELVKAASDELGRTISPMVMAAKGVAGNIQDPGLQKGFLDSGYRILAAVGKVREAFQPQELDFPPPPPDLDQLHVNDDQAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEMVSEPMMVAARSLHDEARKWSSKGNDIIGAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISEEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_021480576.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vinculin a (vcla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480576.1

ORF Insert Sequence:

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAAAGGACA CTGAGGCCAT GAAGAGAGCG CTGGCGCTGA TCGAGTCTAA GATGGGTCAG 
GCTAAAGGCT GGCTGAGGGA CCCCAATGCA CTGCCAGGAG ATCCAGGAGA GCATGCGCTG 
CGTCAGATCC TGGATGAAGC AGGAAAAGTG GGTGAGCTTT GTGCAGGGAA AGAGAGACGA 
GAGATCCTGG GAACAGCCAA AACCCTGGGC CAGATGACGG ATCAGGTGTC AGATGTGCGC 
GCCAGAGGTC AGGGTGCCAC ACCTATGGGT ATGCAGAAAG CTCAGCAGGT TGCTCAAGGT 
TTGGATATTC TGGTGGGAAA AGTGGAGAAC GCAGCCCGTA AGCTGGAGGC CCTAACCAAT 
GCCAAACAGG CCATCGCCAA GAGAATCGAC AATGCCCAGA GCTGGCTGGC TGATCCTAAC 
GGCGGTCCAG AGGGTGAAGA AAACATCCGC GCTCTTCTGG CGGAAGCCAA ACGCATCGCT 
GACTTGTGTG AAGACCCTAA AGAACGAGAT GACATTCTTC GTTCCATCGG CGAGATCGCA 
GGCCTCACCG CCAGGCTGGT CGAGCTCCGT AGAATAGGTA AAGGCGATAC TCCAGAGGCG 
AGAGCTCTTG CCAAACAGAT TGGCACAGCT TTGCAGAACT TACAGGCAAA AACGAACCGT 
GCTGTGGCCA ACATGAGGCC TGCCAAAGCT GCTGTTACCC TGGAGGGCAA AATGGAGCAG 
GCCTTGCGCT GGATCAACAA CCCTGGAGTG GACGATCATG GAGTAGGCCA GGCTGCTATA 
CGAGGGCTGA TAGCAGAGGG TCGACGGCTC GCGAGCTCTC TGCCAGGGCC GTACAGACAG 
GAGCTGCTGG CCAAATGTGA GCAGGTGGAG CAGCTGATGA TGCAGCTGGC GGATCTGGCA 
GCGCGGGGAG AAGGAGAGAG TCCTCAGGCC CGCGCAGTGG CTGCTCATCT TCTGGAGGCA 
ATTAAAGATC TGAAGGCAAA GATGCAGGAA GCAATGACCC AGGAAGTGTC TGATGTTTTC 
AGCGATACAA CAACACCAAT TAAACTCCTG GCTGTGGCAG CAACAGCACC TCTCGAGGCC 
CCGAACAGAG AGGAGGTGTT TGAAGAAAGG GCTTCTAACT TTGAGAATCA TGCTAGCAGG 
CTGGGTGCTA CTGCGGAGAA GGCGGCTGCG GTAGGAACAG CCAATAAAAG CACAGTTGAA 
GGCATCCAAG CGGCTGTGAA ATCAGCTAGA GATCTAACAC CTCAGGTCAC TTCCGCTGCA 
CGTATTTTGC TGAAGAACCC TGGCAACCAG GCCGCCTACG AGCACTTTGA TACCATGAAG 
AACCAGTGGA TTGACAACAT TGAAAAAATG ACCAGTCTTG TGGATGAAGC CATTGACACT 
AAATCCCTCT TGGATGCTTC AGAGGAAGCT ATTAAGAAAG ACATTGATAA ATGCCGTGTA 
GCAATGGCTA ACGTTCAGCC CCAGATGTTG GTTGCAGGGG CTACAAGCAT TGCCCGGCGG 
GCAAACAGAG TACTTCTGGT GGCCAAGAGG GAAGTGGAGA ATTCAGAGGA CCCTAAATTC 
AGAGAACTGG TTAAAGCAGC TTCAGATGAA CTCGGTAGAA CCATCTCACC CATGGTTATG 
GCTGCTAAAG GAGTGGCAGG AAACATCCAG GATCCAGGTT TGCAAAAGGG CTTCCTGGAC 
TCTGGCTACA GGATCTTGGC TGCTGTTGGG AAAGTCAGAG AAGCCTTTCA GCCTCAGGAG 
CTGGACTTTC CTCCGCCACC TCCAGACCTG GATCAGCTGC ATGTCAATGA TGACCAGGCT 
CCACCCAAAC CACCTCTTCC TGAGGGAGAG GTTCCTCCGC CGAGACCTCC ACCTCCAGAA 
GAGAAAGATG AGGAGTTCCC TGAGCAGAAG GCAGGAGAGA TGGTGAGCGA GCCCATGATG 
GTGGCTGCCA GGTCACTGCA CGACGAGGCC CGCAAGTGGT CCAGCAAGGG TAATGACATC 
ATTGGAGCCG CCAAGCGTAT GGCCCTGCTG ATGGCAGAGA TGTCTCGTCT GGTGCGTGGT 
GGAAGTGGGA ATAAACGTGC CCTTATTCAG TGCGCCAAGG ACATCGCCAA GGCCTCTGAT 
GAGGTCACGC GGCTGGCTAA AGAAGTAGCC AAACAATGTA CTGACAAAAG GATTCGCACA 
AACCTGCTGC AGGTTTGTGA GCGCATCCCA ACCATCAGCA CTCAGTTGAA GATCCTTTCC 
ACAGTGAAGG CCACTATGCT GGGACGTACA AATATCAGTG AGGAAGAATC CGAACAGGCC 
ACTGAGATGC TTGTGCACAA CGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACGGTGAGA 
GAAGCAGAAG CCGCTTCCAT CAAGATCCGC ACAGACGCAG GCTTCACACT TCGCTGGGTC 
CGAAAAACCC CCTGGTACCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcla ( NM_001128681.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122153.1 Danio rerio vinculin a (vcla), mRNA.

Price & Availability↑

CloneID	ODa03921
Clone ID Related Accession (Same CDS sequence)	NM_001128681.1
Accession Version	NM_001128681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Danio rerio(zebrafish)
Product	vinculin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162980.1. On or before Jul 31, 2008 this sequence version replaced XM_001340074.2, XM_692978.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC162980.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAAAGGACA CTGAGGCCAT GAAGAGAGCG CTGGCGCTGA TCGAGTCTAA GATGGGTCAG 
GCTAAAGGCT GGCTGAGGGA CCCCAATGCA CTGCCAGGAG ATCCAGGAGA GCATGCGCTG 
CGTCAGATCC TGGATGAAGC AGGAAAAGTG GGTGAGCTTT GTGCAGGGAA AGAGAGACGA 
GAGATCCTGG GAACAGCCAA AACCCTGGGC CAGATGACGG ATCAGGTGTC AGATGTGCGC 
GCCAGAGGTC AGGGTGCCAC ACCTATGGGT ATGCAGAAAG CTCAGCAGGT TGCTCAAGGT 
TTGGATATTC TGGTGGGAAA AGTGGAGAAC GCAGCCCGTA AGCTGGAGGC CCTAACCAAT 
GCCAAACAGG CCATCGCCAA GAGAATCGAC AATGCCCAGA GCTGGCTGGC TGATCCTAAC 
GGCGGTCCAG AGGGTGAAGA AAACATCCGC GCTCTTCTGG CGGAAGCCAA ACGCATCGCT 
GACTTGTGTG AAGACCCTAA AGAACGAGAT GACATTCTTC GTTCCATCGG CGAGATCGCA 
GGCCTCACCG CCAGGCTGGT CGAGCTCCGT AGAATAGGTA AAGGCGATAC TCCAGAGGCG 
AGAGCTCTTG CCAAACAGAT TGGCACAGCT TTGCAGAACT TACAGGCAAA AACGAACCGT 
GCTGTGGCCA ACATGAGGCC TGCCAAAGCT GCTGTTACCC TGGAGGGCAA AATGGAGCAG 
GCCTTGCGCT GGATCAACAA CCCTGGAGTG GACGATCATG GAGTAGGCCA GGCTGCTATA 
CGAGGGCTGA TAGCAGAGGG TCGACGGCTC GCGAGCTCTC TGCCAGGGCC GTACAGACAG 
GAGCTGCTGG CCAAATGTGA GCAGGTGGAG CAGCTGATGA TGCAGCTGGC GGATCTGGCA 
GCGCGGGGAG AAGGAGAGAG TCCTCAGGCC CGCGCAGTGG CTGCTCATCT TCTGGAGGCA 
ATTAAAGATC TGAAGGCAAA GATGCAGGAA GCAATGACCC AGGAAGTGTC TGATGTTTTC 
AGCGATACAA CAACACCAAT TAAACTCCTG GCTGTGGCAG CAACAGCACC TCTCGAGGCC 
CCGAACAGAG AGGAGGTGTT TGAAGAAAGG GCTTCTAACT TTGAGAATCA TGCTAGCAGG 
CTGGGTGCTA CTGCGGAGAA GGCGGCTGCG GTAGGAACAG CCAATAAAAG CACAGTTGAA 
GGCATCCAAG CGGCTGTGAA ATCAGCTAGA GATCTAACAC CTCAGGTCAC TTCCGCTGCA 
CGTATTTTGC TGAAGAACCC TGGCAACCAG GCCGCCTACG AGCACTTTGA TACCATGAAG 
AACCAGTGGA TTGACAACAT TGAAAAAATG ACCAGTCTTG TGGATGAAGC CATTGACACT 
AAATCCCTCT TGGATGCTTC AGAGGAAGCT ATTAAGAAAG ACATTGATAA ATGCCGTGTA 
GCAATGGCTA ACGTTCAGCC CCAGATGTTG GTTGCAGGGG CTACAAGCAT TGCCCGGCGG 
GCAAACAGAG TACTTCTGGT GGCCAAGAGG GAAGTGGAGA ATTCAGAGGA CCCTAAATTC 
AGAGAACTGG TTAAAGCAGC TTCAGATGAA CTCGGTAGAA CCATCTCACC CATGGTTATG 
GCTGCTAAAG GAGTGGCAGG AAACATCCAG GATCCAGGTT TGCAAAAGGG CTTCCTGGAC 
TCTGGCTACA GGATCTTGGC TGCTGTTGGG AAAGTCAGAG AAGCCTTTCA GCCTCAGGAG 
CTGGACTTTC CTCCGCCACC TCCAGACCTG GATCAGCTGC ATGTCAATGA TGACCAGGCT 
CCACCCAAAC CACCTCTTCC TGAGGGAGAG GTTCCTCCGC CGAGACCTCC ACCTCCAGAA 
GAGAAAGATG AGGAGTTCCC TGAGCAGAAG GCAGGAGAGA TGGTGAGCGA GCCCATGATG 
GTGGCTGCCA GGTCACTGCA CGACGAGGCC CGCAAGTGGT CCAGCAAGCC CGAGGTCCCA 
GATGAGGGTT TTGAGGCCGC TGAGGCGGAA GTAGATATAG ACGATGAGGA TGAATTCACA 
GACAACGAGG ACGATTATGA GCCAGAGCTG CTCTTAATGT CTTCCAATCA GCCAGTTAAC 
CAGCCCATTC TGGCCGCTGC GCAGTCTTTG CACCAGGAGG CTCGCAAGTG GTCCAGTAAG 
GGTAATGACA TCATTGGAGC CGCCAAGCGT ATGGCCCTGC TGATGGCAGA GATGTCTCGT 
CTGGTGCGTG GTGGAAGTGG GAATAAACGT GCCCTTATTC AGTGCGCCAA GGACATCGCC 
AAGGCCTCTG ATGAGGTCAC GCGGCTGGCT AAAGAAGTAG CCAAACAATG TACTGACAAA 
AGGATTCGCA CAAACCTGCT GCAGGTTTGT GAGCGCATCC CAACCATCAG CACTCAGTTG 
AAGATCCTTT CCACAGTGAA GGCCACTATG CTGGGACGTA CAAATATCAG TGAGGAAGAA 
TCCGAACAGG CCACTGAGAT GCTTGTGCAC AACGCCCAGA ACCTCATGCA GTCTGTGAAG 
GAGACGGTGA GAGAAGCAGA AGCCGCTTCC ATCAAGATCC GCACAGACGC AGGCTTCACA 
CTTCGCTGGG TCCGAAAAAC CCCCTGGTAC CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122153.1
CDS	89..3484
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTSPVAAVQAAVSNLVRVGKETVQTTEDKIMKRDMPSAFIKVENACAKLVEAAQMLRTDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLITYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVTSMNTVKELLPVLISAIKIFVTTKCTKSHGVEEALKNRNYTFDKMTAEINEIIRVLQLTSWDEDAWANKKDTEAMKRALALIESKMGQAKGWLRDPNALPGDPGEHALRQILDEAGKVGELCAGKERREILGTAKTLGQMTDQVSDVRARGQGATPMGMQKAQQVAQGLDILVGKVENAARKLEALTNAKQAIAKRIDNAQSWLADPNGGPEGEENIRALLAEAKRIADLCEDPKERDDILRSIGEIAGLTARLVELRRIGKGDTPEARALAKQIGTALQNLQAKTNRAVANMRPAKAAVTLEGKMEQALRWINNPGVDDHGVGQAAIRGLIAEGRRLASSLPGPYRQELLAKCEQVEQLMMQLADLAARGEGESPQARAVAAHLLEAIKDLKAKMQEAMTQEVSDVFSDTTTPIKLLAVAATAPLEAPNREEVFEERASNFENHASRLGATAEKAAAVGTANKSTVEGIQAAVKSARDLTPQVTSAARILLKNPGNQAAYEHFDTMKNQWIDNIEKMTSLVDEAIDTKSLLDASEEAIKKDIDKCRVAMANVQPQMLVAGATSIARRANRVLLVAKREVENSEDPKFRELVKAASDELGRTISPMVMAAKGVAGNIQDPGLQKGFLDSGYRILAAVGKVREAFQPQELDFPPPPPDLDQLHVNDDQAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEMVSEPMMVAARSLHDEARKWSSKPEVPDEGFEAAEAEVDIDDEDEFTDNEDDYEPELLLMSSNQPVNQPILAAAQSLHQEARKWSSKGNDIIGAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISEEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_001128681.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vinculin a (vcla), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128681.1

ORF Insert Sequence:

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAAAGGACA CTGAGGCCAT GAAGAGAGCG CTGGCGCTGA TCGAGTCTAA GATGGGTCAG 
GCTAAAGGCT GGCTGAGGGA CCCCAATGCA CTGCCAGGAG ATCCAGGAGA GCATGCGCTG 
CGTCAGATCC TGGATGAAGC AGGAAAAGTG GGTGAGCTTT GTGCAGGGAA AGAGAGACGA 
GAGATCCTGG GAACAGCCAA AACCCTGGGC CAGATGACGG ATCAGGTGTC AGATGTGCGC 
GCCAGAGGTC AGGGTGCCAC ACCTATGGGT ATGCAGAAAG CTCAGCAGGT TGCTCAAGGT 
TTGGATATTC TGGTGGGAAA AGTGGAGAAC GCAGCCCGTA AGCTGGAGGC CCTAACCAAT 
GCCAAACAGG CCATCGCCAA GAGAATCGAC AATGCCCAGA GCTGGCTGGC TGATCCTAAC 
GGCGGTCCAG AGGGTGAAGA AAACATCCGC GCTCTTCTGG CGGAAGCCAA ACGCATCGCT 
GACTTGTGTG AAGACCCTAA AGAACGAGAT GACATTCTTC GTTCCATCGG CGAGATCGCA 
GGCCTCACCG CCAGGCTGGT CGAGCTCCGT AGAATAGGTA AAGGCGATAC TCCAGAGGCG 
AGAGCTCTTG CCAAACAGAT TGGCACAGCT TTGCAGAACT TACAGGCAAA AACGAACCGT 
GCTGTGGCCA ACATGAGGCC TGCCAAAGCT GCTGTTACCC TGGAGGGCAA AATGGAGCAG 
GCCTTGCGCT GGATCAACAA CCCTGGAGTG GACGATCATG GAGTAGGCCA GGCTGCTATA 
CGAGGGCTGA TAGCAGAGGG TCGACGGCTC GCGAGCTCTC TGCCAGGGCC GTACAGACAG 
GAGCTGCTGG CCAAATGTGA GCAGGTGGAG CAGCTGATGA TGCAGCTGGC GGATCTGGCA 
GCGCGGGGAG AAGGAGAGAG TCCTCAGGCC CGCGCAGTGG CTGCTCATCT TCTGGAGGCA 
ATTAAAGATC TGAAGGCAAA GATGCAGGAA GCAATGACCC AGGAAGTGTC TGATGTTTTC 
AGCGATACAA CAACACCAAT TAAACTCCTG GCTGTGGCAG CAACAGCACC TCTCGAGGCC 
CCGAACAGAG AGGAGGTGTT TGAAGAAAGG GCTTCTAACT TTGAGAATCA TGCTAGCAGG 
CTGGGTGCTA CTGCGGAGAA GGCGGCTGCG GTAGGAACAG CCAATAAAAG CACAGTTGAA 
GGCATCCAAG CGGCTGTGAA ATCAGCTAGA GATCTAACAC CTCAGGTCAC TTCCGCTGCA 
CGTATTTTGC TGAAGAACCC TGGCAACCAG GCCGCCTACG AGCACTTTGA TACCATGAAG 
AACCAGTGGA TTGACAACAT TGAAAAAATG ACCAGTCTTG TGGATGAAGC CATTGACACT 
AAATCCCTCT TGGATGCTTC AGAGGAAGCT ATTAAGAAAG ACATTGATAA ATGCCGTGTA 
GCAATGGCTA ACGTTCAGCC CCAGATGTTG GTTGCAGGGG CTACAAGCAT TGCCCGGCGG 
GCAAACAGAG TACTTCTGGT GGCCAAGAGG GAAGTGGAGA ATTCAGAGGA CCCTAAATTC 
AGAGAACTGG TTAAAGCAGC TTCAGATGAA CTCGGTAGAA CCATCTCACC CATGGTTATG 
GCTGCTAAAG GAGTGGCAGG AAACATCCAG GATCCAGGTT TGCAAAAGGG CTTCCTGGAC 
TCTGGCTACA GGATCTTGGC TGCTGTTGGG AAAGTCAGAG AAGCCTTTCA GCCTCAGGAG 
CTGGACTTTC CTCCGCCACC TCCAGACCTG GATCAGCTGC ATGTCAATGA TGACCAGGCT 
CCACCCAAAC CACCTCTTCC TGAGGGAGAG GTTCCTCCGC CGAGACCTCC ACCTCCAGAA 
GAGAAAGATG AGGAGTTCCC TGAGCAGAAG GCAGGAGAGA TGGTGAGCGA GCCCATGATG 
GTGGCTGCCA GGTCACTGCA CGACGAGGCC CGCAAGTGGT CCAGCAAGCC CGAGGTCCCA 
GATGAGGGTT TTGAGGCCGC TGAGGCGGAA GTAGATATAG ACGATGAGGA TGAATTCACA 
GACAACGAGG ACGATTATGA GCCAGAGCTG CTCTTAATGT CTTCCAATCA GCCAGTTAAC 
CAGCCCATTC TGGCCGCTGC GCAGTCTTTG CACCAGGAGG CTCGCAAGTG GTCCAGTAAG 
GGTAATGACA TCATTGGAGC CGCCAAGCGT ATGGCCCTGC TGATGGCAGA GATGTCTCGT 
CTGGTGCGTG GTGGAAGTGG GAATAAACGT GCCCTTATTC AGTGCGCCAA GGACATCGCC 
AAGGCCTCTG ATGAGGTCAC GCGGCTGGCT AAAGAAGTAG CCAAACAATG TACTGACAAA 
AGGATTCGCA CAAACCTGCT GCAGGTTTGT GAGCGCATCC CAACCATCAG CACTCAGTTG 
AAGATCCTTT CCACAGTGAA GGCCACTATG CTGGGACGTA CAAATATCAG TGAGGAAGAA 
TCCGAACAGG CCACTGAGAT GCTTGTGCAC AACGCCCAGA ACCTCATGCA GTCTGTGAAG 
GAGACGGTGA GAGAAGCAGA AGCCGCTTCC ATCAAGATCC GCACAGACGC AGGCTTCACA 
CTTCGCTGGG TCCGAAAAAC CCCCTGGTAC CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcla ( XM_005156840.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156897.1 Danio rerio vinculin a (vcla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18783
Clone ID Related Accession (Same CDS sequence)	XM_005156840.3 , XM_005156840.4
Accession Version	XM_005156840.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	vinculin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGCCCGA GGTCCCAGAT 
GAGGGTTTTG AGGCCGCTGA GGCGGAAGTA GATATAGACG ATGAGGATGA ATTCACAGAC 
AACGAGGACG ATTATGAGCC AGAGCTGCTC TTAATGTCTT CCAATCAGCC AGTTAACCAG 
CCCATTCTGG CCGCTGCGCA GTCTTTGCAC CAGGAGGCTC GCAAGTGGTC CAGTAAGGGT 
AATGACATCA TTGGAGCCGC CAAGCGTATG GCCCTGCTGA TGGCAGAGAT GTCTCGTCTG 
GTGCGTGGTG GAAGTGGGAA TAAACGTGCC CTTATTCAGT GCGCCAAGGA CATCGCCAAG 
GCCTCTGATG AGGTCACGCG GCTGGCTAAA GAAGTAGCCA AACAATGTAC TGACAAAAGG 
ATTCGCACAA ACCTGCTGCA GGTTTGTGAG CGCATCCCAA CCATCAGCAC TCAGTTGAAG 
ATCCTTTCCA CAGTGAAGGC CACTATGCTG GGACGTACAA ATATCAGTGA GGAAGAATCC 
GAACAGGCCA CTGAGATGCT TGTGCACAAC GCCCAGAACC TCATGCAGTC TGTGAAGGAG 
ACGGTGAGAG AAGCAGAAGC CGCTTCCATC AAGATCCGCA CAGACGCAGG CTTCACACTT 
CGCTGGGTCC GAAAAACCCC CTGGTACCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156897.1
CDS	209..3601
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTSPVAAVQAAVSNLVRVGKETVQTTEDKIMKRDMPSAFIKVENACAKLVEAAQMLRTDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLITYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVTSMNTVKELLPVLISAIKIFVTTKCTKSHGVEEALKNRNYTFDKMTAEINEIIRVLQLTSWDEDAWANKDTEAMKRALALIESKMGQAKGWLRDPNALPGDPGEHALRQILDEAGKVGELCAGKERREILGTAKTLGQMTDQVSDVRARGQGATPMGMQKAQQVAQGLDILVGKVENAARKLEALTNAKQAIAKRIDNAQSWLADPNGGPEGEENIRALLAEAKRIADLCEDPKERDDILRSIGEIAGLTARLVELRRIGKGDTPEARALAKQIGTALQNLQAKTNRAVANMRPAKAAVTLEGKMEQALRWINNPGVDDHGVGQAAIRGLIAEGRRLASSLPGPYRQELLAKCEQVEQLMMQLADLAARGEGESPQARAVAAHLLEAIKDLKAKMQEAMTQEVSDVFSDTTTPIKLLAVAATAPLEAPNREEVFEERASNFENHASRLGATAEKAAAVGTANKSTVEGIQAAVKSARDLTPQVTSAARILLKNPGNQAAYEHFDTMKNQWIDNIEKMTSLVDEAIDTKSLLDASEEAIKKDIDKCRVAMANVQPQMLVAGATSIARRANRVLLVAKREVENSEDPKFRELVKAASDELGRTISPMVMAAKGVAGNIQDPGLQKGFLDSGYRILAAVGKVREAFQPQELDFPPPPPDLDQLHVNDDQAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEMVSEPMMVAARSLHDEARKWSSKPEVPDEGFEAAEAEVDIDDEDEFTDNEDDYEPELLLMSSNQPVNQPILAAAQSLHQEARKWSSKGNDIIGAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISEEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005156840.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vinculin a (vcla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156840.3

ORF Insert Sequence:

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGCCCGA GGTCCCAGAT 
GAGGGTTTTG AGGCCGCTGA GGCGGAAGTA GATATAGACG ATGAGGATGA ATTCACAGAC 
AACGAGGACG ATTATGAGCC AGAGCTGCTC TTAATGTCTT CCAATCAGCC AGTTAACCAG 
CCCATTCTGG CCGCTGCGCA GTCTTTGCAC CAGGAGGCTC GCAAGTGGTC CAGTAAGGGT 
AATGACATCA TTGGAGCCGC CAAGCGTATG GCCCTGCTGA TGGCAGAGAT GTCTCGTCTG 
GTGCGTGGTG GAAGTGGGAA TAAACGTGCC CTTATTCAGT GCGCCAAGGA CATCGCCAAG 
GCCTCTGATG AGGTCACGCG GCTGGCTAAA GAAGTAGCCA AACAATGTAC TGACAAAAGG 
ATTCGCACAA ACCTGCTGCA GGTTTGTGAG CGCATCCCAA CCATCAGCAC TCAGTTGAAG 
ATCCTTTCCA CAGTGAAGGC CACTATGCTG GGACGTACAA ATATCAGTGA GGAAGAATCC 
GAACAGGCCA CTGAGATGCT TGTGCACAAC GCCCAGAACC TCATGCAGTC TGTGAAGGAG 
ACGGTGAGAG AAGCAGAAGC CGCTTCCATC AAGATCCGCA CAGACGCAGG CTTCACACTT 
CGCTGGGTCC GAAAAACCCC CTGGTACCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcla ( XM_005156840.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156897.1 Danio rerio vinculin a (vcla), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa18783
Clone ID Related Accession (Same CDS sequence)	XM_005156840.3 , XM_005156840.4
Accession Version	XM_005156840.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156840.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGCCCGA GGTCCCAGAT 
GAGGGTTTTG AGGCCGCTGA GGCGGAAGTA GATATAGACG ATGAGGATGA ATTCACAGAC 
AACGAGGACG ATTATGAGCC AGAGCTGCTC TTAATGTCTT CCAATCAGCC AGTTAACCAG 
CCCATTCTGG CCGCTGCGCA GTCTTTGCAC CAGGAGGCTC GCAAGTGGTC CAGTAAGGGT 
AATGACATCA TTGGAGCCGC CAAGCGTATG GCCCTGCTGA TGGCAGAGAT GTCTCGTCTG 
GTGCGTGGTG GAAGTGGGAA TAAACGTGCC CTTATTCAGT GCGCCAAGGA CATCGCCAAG 
GCCTCTGATG AGGTCACGCG GCTGGCTAAA GAAGTAGCCA AACAATGTAC TGACAAAAGG 
ATTCGCACAA ACCTGCTGCA GGTTTGTGAG CGCATCCCAA CCATCAGCAC TCAGTTGAAG 
ATCCTTTCCA CAGTGAAGGC CACTATGCTG GGACGTACAA ATATCAGTGA GGAAGAATCC 
GAACAGGCCA CTGAGATGCT TGTGCACAAC GCCCAGAACC TCATGCAGTC TGTGAAGGAG 
ACGGTGAGAG AAGCAGAAGC CGCTTCCATC AAGATCCGCA CAGACGCAGG CTTCACACTT 
CGCTGGGTCC GAAAAACCCC CTGGTACCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156897.1
CDS	218..3610
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTSPVAAVQAAVSNLVRVGKETVQTTEDKIMKRDMPSAFIKVENACAKLVEAAQMLRTDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLITYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVTSMNTVKELLPVLISAIKIFVTTKCTKSHGVEEALKNRNYTFDKMTAEINEIIRVLQLTSWDEDAWANKDTEAMKRALALIESKMGQAKGWLRDPNALPGDPGEHALRQILDEAGKVGELCAGKERREILGTAKTLGQMTDQVSDVRARGQGATPMGMQKAQQVAQGLDILVGKVENAARKLEALTNAKQAIAKRIDNAQSWLADPNGGPEGEENIRALLAEAKRIADLCEDPKERDDILRSIGEIAGLTARLVELRRIGKGDTPEARALAKQIGTALQNLQAKTNRAVANMRPAKAAVTLEGKMEQALRWINNPGVDDHGVGQAAIRGLIAEGRRLASSLPGPYRQELLAKCEQVEQLMMQLADLAARGEGESPQARAVAAHLLEAIKDLKAKMQEAMTQEVSDVFSDTTTPIKLLAVAATAPLEAPNREEVFEERASNFENHASRLGATAEKAAAVGTANKSTVEGIQAAVKSARDLTPQVTSAARILLKNPGNQAAYEHFDTMKNQWIDNIEKMTSLVDEAIDTKSLLDASEEAIKKDIDKCRVAMANVQPQMLVAGATSIARRANRVLLVAKREVENSEDPKFRELVKAASDELGRTISPMVMAAKGVAGNIQDPGLQKGFLDSGYRILAAVGKVREAFQPQELDFPPPPPDLDQLHVNDDQAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEMVSEPMMVAARSLHDEARKWSSKPEVPDEGFEAAEAEVDIDDEDEFTDNEDDYEPELLLMSSNQPVNQPILAAAQSLHQEARKWSSKGNDIIGAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISEEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005156840.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vinculin a (vcla), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156840.4

ORF Insert Sequence:

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGCCCGA GGTCCCAGAT 
GAGGGTTTTG AGGCCGCTGA GGCGGAAGTA GATATAGACG ATGAGGATGA ATTCACAGAC 
AACGAGGACG ATTATGAGCC AGAGCTGCTC TTAATGTCTT CCAATCAGCC AGTTAACCAG 
CCCATTCTGG CCGCTGCGCA GTCTTTGCAC CAGGAGGCTC GCAAGTGGTC CAGTAAGGGT 
AATGACATCA TTGGAGCCGC CAAGCGTATG GCCCTGCTGA TGGCAGAGAT GTCTCGTCTG 
GTGCGTGGTG GAAGTGGGAA TAAACGTGCC CTTATTCAGT GCGCCAAGGA CATCGCCAAG 
GCCTCTGATG AGGTCACGCG GCTGGCTAAA GAAGTAGCCA AACAATGTAC TGACAAAAGG 
ATTCGCACAA ACCTGCTGCA GGTTTGTGAG CGCATCCCAA CCATCAGCAC TCAGTTGAAG 
ATCCTTTCCA CAGTGAAGGC CACTATGCTG GGACGTACAA ATATCAGTGA GGAAGAATCC 
GAACAGGCCA CTGAGATGCT TGTGCACAAC GCCCAGAACC TCATGCAGTC TGTGAAGGAG 
ACGGTGAGAG AAGCAGAAGC CGCTTCCATC AAGATCCGCA CAGACGCAGG CTTCACACTT 
CGCTGGGTCC GAAAAACCCC CTGGTACCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcla ( XM_005156842.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156899.1 Danio rerio vinculin a (vcla), transcript variant X2, mRNA.

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CloneID	ODa18785
Clone ID Related Accession (Same CDS sequence)	XM_005156842.3 , XM_005156842.4
Accession Version	XM_005156842.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	vinculin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156842.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGGGTAA TGACATCATT 
GGAGCCGCCA AGCGTATGGC CCTGCTGATG GCAGAGATGT CTCGTCTGGT GCGTGGTGGA 
AGTGGGAATA AACGTGCCCT TATTCAGTGC GCCAAGGACA TCGCCAAGGC CTCTGATGAG 
GTCACGCGGC TGGCTAAAGA AGTAGCCAAA CAATGTACTG ACAAAAGGAT TCGCACAAAC 
CTGCTGCAGG TTTGTGAGCG CATCCCAACC ATCAGCACTC AGTTGAAGAT CCTTTCCACA 
GTGAAGGCCA CTATGCTGGG ACGTACAAAT ATCAGTGAGG AAGAATCCGA ACAGGCCACT 
GAGATGCTTG TGCACAACGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC GGTGAGAGAA 
GCAGAAGCCG CTTCCATCAA GATCCGCACA GACGCAGGCT TCACACTTCG CTGGGTCCGA 
AAAACCCCCT GGTACCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156899.1
CDS	209..3409
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTSPVAAVQAAVSNLVRVGKETVQTTEDKIMKRDMPSAFIKVENACAKLVEAAQMLRTDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLITYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVTSMNTVKELLPVLISAIKIFVTTKCTKSHGVEEALKNRNYTFDKMTAEINEIIRVLQLTSWDEDAWANKDTEAMKRALALIESKMGQAKGWLRDPNALPGDPGEHALRQILDEAGKVGELCAGKERREILGTAKTLGQMTDQVSDVRARGQGATPMGMQKAQQVAQGLDILVGKVENAARKLEALTNAKQAIAKRIDNAQSWLADPNGGPEGEENIRALLAEAKRIADLCEDPKERDDILRSIGEIAGLTARLVELRRIGKGDTPEARALAKQIGTALQNLQAKTNRAVANMRPAKAAVTLEGKMEQALRWINNPGVDDHGVGQAAIRGLIAEGRRLASSLPGPYRQELLAKCEQVEQLMMQLADLAARGEGESPQARAVAAHLLEAIKDLKAKMQEAMTQEVSDVFSDTTTPIKLLAVAATAPLEAPNREEVFEERASNFENHASRLGATAEKAAAVGTANKSTVEGIQAAVKSARDLTPQVTSAARILLKNPGNQAAYEHFDTMKNQWIDNIEKMTSLVDEAIDTKSLLDASEEAIKKDIDKCRVAMANVQPQMLVAGATSIARRANRVLLVAKREVENSEDPKFRELVKAASDELGRTISPMVMAAKGVAGNIQDPGLQKGFLDSGYRILAAVGKVREAFQPQELDFPPPPPDLDQLHVNDDQAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEMVSEPMMVAARSLHDEARKWSSKGNDIIGAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISEEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005156842.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio vinculin a (vcla), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156842.3

ORF Insert Sequence:

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGGGTAA TGACATCATT 
GGAGCCGCCA AGCGTATGGC CCTGCTGATG GCAGAGATGT CTCGTCTGGT GCGTGGTGGA 
AGTGGGAATA AACGTGCCCT TATTCAGTGC GCCAAGGACA TCGCCAAGGC CTCTGATGAG 
GTCACGCGGC TGGCTAAAGA AGTAGCCAAA CAATGTACTG ACAAAAGGAT TCGCACAAAC 
CTGCTGCAGG TTTGTGAGCG CATCCCAACC ATCAGCACTC AGTTGAAGAT CCTTTCCACA 
GTGAAGGCCA CTATGCTGGG ACGTACAAAT ATCAGTGAGG AAGAATCCGA ACAGGCCACT 
GAGATGCTTG TGCACAACGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC GGTGAGAGAA 
GCAGAAGCCG CTTCCATCAA GATCCGCACA GACGCAGGCT TCACACTTCG CTGGGTCCGA 
AAAACCCCCT GGTACCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vcla ( XM_005156842.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005156899.1 Danio rerio vinculin a (vcla), transcript variant X3, mRNA.

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CloneID	ODa18785
Clone ID Related Accession (Same CDS sequence)	XM_005156842.3 , XM_005156842.4
Accession Version	XM_005156842.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156842.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGGGTAA TGACATCATT 
GGAGCCGCCA AGCGTATGGC CCTGCTGATG GCAGAGATGT CTCGTCTGGT GCGTGGTGGA 
AGTGGGAATA AACGTGCCCT TATTCAGTGC GCCAAGGACA TCGCCAAGGC CTCTGATGAG 
GTCACGCGGC TGGCTAAAGA AGTAGCCAAA CAATGTACTG ACAAAAGGAT TCGCACAAAC 
CTGCTGCAGG TTTGTGAGCG CATCCCAACC ATCAGCACTC AGTTGAAGAT CCTTTCCACA 
GTGAAGGCCA CTATGCTGGG ACGTACAAAT ATCAGTGAGG AAGAATCCGA ACAGGCCACT 
GAGATGCTTG TGCACAACGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC GGTGAGAGAA 
GCAGAAGCCG CTTCCATCAA GATCCGCACA GACGCAGGCT TCACACTTCG CTGGGTCCGA 
AAAACCCCCT GGTACCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005156899.1
CDS	217..3417
Translation	MPVFHTKTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTSPVAAVQAAVSNLVRVGKETVQTTEDKIMKRDMPSAFIKVENACAKLVEAAQMLRTDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLITYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVTSMNTVKELLPVLISAIKIFVTTKCTKSHGVEEALKNRNYTFDKMTAEINEIIRVLQLTSWDEDAWANKDTEAMKRALALIESKMGQAKGWLRDPNALPGDPGEHALRQILDEAGKVGELCAGKERREILGTAKTLGQMTDQVSDVRARGQGATPMGMQKAQQVAQGLDILVGKVENAARKLEALTNAKQAIAKRIDNAQSWLADPNGGPEGEENIRALLAEAKRIADLCEDPKERDDILRSIGEIAGLTARLVELRRIGKGDTPEARALAKQIGTALQNLQAKTNRAVANMRPAKAAVTLEGKMEQALRWINNPGVDDHGVGQAAIRGLIAEGRRLASSLPGPYRQELLAKCEQVEQLMMQLADLAARGEGESPQARAVAAHLLEAIKDLKAKMQEAMTQEVSDVFSDTTTPIKLLAVAATAPLEAPNREEVFEERASNFENHASRLGATAEKAAAVGTANKSTVEGIQAAVKSARDLTPQVTSAARILLKNPGNQAAYEHFDTMKNQWIDNIEKMTSLVDEAIDTKSLLDASEEAIKKDIDKCRVAMANVQPQMLVAGATSIARRANRVLLVAKREVENSEDPKFRELVKAASDELGRTISPMVMAAKGVAGNIQDPGLQKGFLDSGYRILAAVGKVREAFQPQELDFPPPPPDLDQLHVNDDQAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEMVSEPMMVAARSLHDEARKWSSKGNDIIGAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISEEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_005156842.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vinculin a (vcla), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005156842.4

ORF Insert Sequence:

ATGCCAGTTT TCCATACCAA GACGATAGAG AGTATCCTGG AGCCGGTGGC TCAGCAGATT 
TCACACTTGG TTATCATGCA CGAGGAGGGA GAAGTTGATG GGAAAGCGAT CCCAGATCTC 
ACCAGCCCAG TGGCGGCAGT GCAAGCGGCT GTCAGCAACC TTGTGCGGGT GGGGAAGGAG 
ACAGTGCAGA CCACAGAAGA CAAGATTATG AAGAGAGACA TGCCATCAGC CTTCATTAAG 
GTGGAGAACG CCTGTGCAAA GCTGGTGGAA GCAGCTCAGA TGCTGAGAAC AGACCCGTAC 
TCTGTCCCGG CCCGTGATTA CCTCATCGAC GGCTCTCGTG GAATCCTCTC AGGCACCTCA 
GACCTGCTGC TGACCTTCGA TGAAGCCGAG GTGCGTAAAA TTATCCGCGT GTGTAAGGGC 
ATCCTGGAAT ACCTGACTGT TGCAGAGGTT GTGGAAACCA TGGAGGACTT GATCACTTAC 
ACTAAAAACC TGGGACCAGG CATGACGAAG ATGGCAAAGA TGATTGACGA GCGGCAGCAG 
GAACTCACGC ACCAGGAGCA CCGCGTAATG TTGGTCACCT CCATGAACAC AGTAAAGGAG 
CTGCTGCCCG TGCTTATATC AGCCATTAAA ATCTTTGTGA CAACCAAGTG CACTAAGAGT 
CACGGTGTGG AGGAGGCCTT GAAGAACAGA AACTACACGT TTGATAAGAT GACAGCTGAA 
ATCAACGAGA TAATCAGAGT GCTGCAGCTC ACTTCATGGG ACGAGGACGC CTGGGCAAAT 
AAAGACACTG AGGCCATGAA GAGAGCGCTG GCGCTGATCG AGTCTAAGAT GGGTCAGGCT 
AAAGGCTGGC TGAGGGACCC CAATGCACTG CCAGGAGATC CAGGAGAGCA TGCGCTGCGT 
CAGATCCTGG ATGAAGCAGG AAAAGTGGGT GAGCTTTGTG CAGGGAAAGA GAGACGAGAG 
ATCCTGGGAA CAGCCAAAAC CCTGGGCCAG ATGACGGATC AGGTGTCAGA TGTGCGCGCC 
AGAGGTCAGG GTGCCACACC TATGGGTATG CAGAAAGCTC AGCAGGTTGC TCAAGGTTTG 
GATATTCTGG TGGGAAAAGT GGAGAACGCA GCCCGTAAGC TGGAGGCCCT AACCAATGCC 
AAACAGGCCA TCGCCAAGAG AATCGACAAT GCCCAGAGCT GGCTGGCTGA TCCTAACGGC 
GGTCCAGAGG GTGAAGAAAA CATCCGCGCT CTTCTGGCGG AAGCCAAACG CATCGCTGAC 
TTGTGTGAAG ACCCTAAAGA ACGAGATGAC ATTCTTCGTT CCATCGGCGA GATCGCAGGC 
CTCACCGCCA GGCTGGTCGA GCTCCGTAGA ATAGGTAAAG GCGATACTCC AGAGGCGAGA 
GCTCTTGCCA AACAGATTGG CACAGCTTTG CAGAACTTAC AGGCAAAAAC GAACCGTGCT 
GTGGCCAACA TGAGGCCTGC CAAAGCTGCT GTTACCCTGG AGGGCAAAAT GGAGCAGGCC 
TTGCGCTGGA TCAACAACCC TGGAGTGGAC GATCATGGAG TAGGCCAGGC TGCTATACGA 
GGGCTGATAG CAGAGGGTCG ACGGCTCGCG AGCTCTCTGC CAGGGCCGTA CAGACAGGAG 
CTGCTGGCCA AATGTGAGCA GGTGGAGCAG CTGATGATGC AGCTGGCGGA TCTGGCAGCG 
CGGGGAGAAG GAGAGAGTCC TCAGGCCCGC GCAGTGGCTG CTCATCTTCT GGAGGCAATT 
AAAGATCTGA AGGCAAAGAT GCAGGAAGCA ATGACCCAGG AAGTGTCTGA TGTTTTCAGC 
GATACAACAA CACCAATTAA ACTCCTGGCT GTGGCAGCAA CAGCACCTCT CGAGGCCCCG 
AACAGAGAGG AGGTGTTTGA AGAAAGGGCT TCTAACTTTG AGAATCATGC TAGCAGGCTG 
GGTGCTACTG CGGAGAAGGC GGCTGCGGTA GGAACAGCCA ATAAAAGCAC AGTTGAAGGC 
ATCCAAGCGG CTGTGAAATC AGCTAGAGAT CTAACACCTC AGGTCACTTC CGCTGCACGT 
ATTTTGCTGA AGAACCCTGG CAACCAGGCC GCCTACGAGC ACTTTGATAC CATGAAGAAC 
CAGTGGATTG ACAACATTGA AAAAATGACC AGTCTTGTGG ATGAAGCCAT TGACACTAAA 
TCCCTCTTGG ATGCTTCAGA GGAAGCTATT AAGAAAGACA TTGATAAATG CCGTGTAGCA 
ATGGCTAACG TTCAGCCCCA GATGTTGGTT GCAGGGGCTA CAAGCATTGC CCGGCGGGCA 
AACAGAGTAC TTCTGGTGGC CAAGAGGGAA GTGGAGAATT CAGAGGACCC TAAATTCAGA 
GAACTGGTTA AAGCAGCTTC AGATGAACTC GGTAGAACCA TCTCACCCAT GGTTATGGCT 
GCTAAAGGAG TGGCAGGAAA CATCCAGGAT CCAGGTTTGC AAAAGGGCTT CCTGGACTCT 
GGCTACAGGA TCTTGGCTGC TGTTGGGAAA GTCAGAGAAG CCTTTCAGCC TCAGGAGCTG 
GACTTTCCTC CGCCACCTCC AGACCTGGAT CAGCTGCATG TCAATGATGA CCAGGCTCCA 
CCCAAACCAC CTCTTCCTGA GGGAGAGGTT CCTCCGCCGA GACCTCCACC TCCAGAAGAG 
AAAGATGAGG AGTTCCCTGA GCAGAAGGCA GGAGAGATGG TGAGCGAGCC CATGATGGTG 
GCTGCCAGGT CACTGCACGA CGAGGCCCGC AAGTGGTCCA GCAAGGGTAA TGACATCATT 
GGAGCCGCCA AGCGTATGGC CCTGCTGATG GCAGAGATGT CTCGTCTGGT GCGTGGTGGA 
AGTGGGAATA AACGTGCCCT TATTCAGTGC GCCAAGGACA TCGCCAAGGC CTCTGATGAG 
GTCACGCGGC TGGCTAAAGA AGTAGCCAAA CAATGTACTG ACAAAAGGAT TCGCACAAAC 
CTGCTGCAGG TTTGTGAGCG CATCCCAACC ATCAGCACTC AGTTGAAGAT CCTTTCCACA 
GTGAAGGCCA CTATGCTGGG ACGTACAAAT ATCAGTGAGG AAGAATCCGA ACAGGCCACT 
GAGATGCTTG TGCACAACGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC GGTGAGAGAA 
GCAGAAGCCG CTTCCATCAA GATCCGCACA GACGCAGGCT TCACACTTCG CTGGGTCCGA 
AAAACCCCCT GGTACCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Zygotic vinculin is not essential for embryonic development in zebrafish.
PloS one12(8)e0182278(2017)
Han MKL,van der Krogt GNM,de Rooij J

Identification and Expression Analysis of the Complete Family of Zebrafish
Frontiers in cell and developmental biology55(2017)
England SJ,Campbell PC,Banerjee S,Swanson AJ,Lewis KE

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vcla cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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