poli cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	poli
Entrez Gene ID	550532
Full Name	polymerase (DNA directed) iota
Synonyms	wu:fc27h11,zgc:110185
General protein information	Preferred Names polymerase (DNA directed) iota Names DNA polymerase iota
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301304.1	XP_009299579.1	DNA polymerase iota isoform X3
XM_017356083.1	XP_017211572.1	DNA polymerase iota isoform X1
XM_017356084.2	XP_017211573.1	DNA polymerase iota isoform X1
XM_017356084.1	XP_017211573.1	DNA polymerase iota isoform X2
NM_001017834.1	NP_001017834.1	DNA polymerase iota

Pathways from BioSystems

Homologies

Bos taurus (cattle)	POLI	NP_001019863.1
Pan troglodytes (chimpanzee)	POLI	XP_001156755.1
Rattus norvegicus (Norway rat)	Poli	NP_001099607.1
Danio rerio (zebrafish)	poli	NP_001017834.1
Homo sapiens (human)	POLI	NP_009126.2
Canis lupus familiaris (dog)	POLI	XP_005615447.1
Mus musculus (house mouse)	Poli	NP_036102.2
Xenopus tropicalis (tropical clawed frog)	poli	NP_001027479.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Transcriptome analysis reveals positive selection on the divergent between topmouth culter and zebrafish.
Ren L, Tan XJ, Xiong YF, Xu K, Zhou Y, Zhong H, Liu Y, Hong YH, Liu SJ
Gene552(2)265-71(2014 Dec)

Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary.
Zeng S, Gong Z
Gene294(1-2)45-53(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following poli gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the poli cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa41790	XM_009301304.1 Latest version!	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa32650	NM_001017834.1 Latest version!	Danio rerio polymerase (DNA directed) iota (poli), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa59079	XM_017356083.1 Latest version!	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa59080	XM_017356084.2 Latest version!	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa59080	XM_017356084.1	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

poli ( XM_009301304.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299579.1 Danio rerio polymerase (DNA directed) iota (poli), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa41790
Clone ID Related Accession (Same CDS sequence)	XM_009301304.1
Accession Version	XM_009301304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase iota isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCGAA ATCCAGCTCT CCGGACCAAA CCTCTTGGGA TACAGCAGAA ATATATTATT 
GTAACCAGTA ATTATGTGGC AAGAGAGCTT GGCGTGAACA AGCTGATGTC AGTGACAGAT 
GCAGTGGAGA AATGTCCACA GCTGGTGCTT GTAAAAGGAG AAGATCTCAC TCATTACAGA 
GAAATGTCCT ACAAAGTCAC TGAATTGCTG ATGTCGTACT GCCCTCTAGT GGAGCGGCTG 
GGCTTTGATG AGAACTTCAT GGACGTTACA GAAATGGTGG AGAAACGAAT AAAGGAGACC 
CGTATTTCAG ATCTCTCCTT CAATGGACAC GTATACAATC ATGAAAGTGT TGTGGTTGAT 
GAGGAGAACA TTCGTTTGGC TGTCGGCTCT GTGATTGCCG CTGAGATGAG GCAAGCCATC 
TTCAGCACAC TGGGGCTAAC TGGCTGTGCT GGTGTCGCTA GCAACAAACT ATTAGCCAAG 
CTGGTGTCAG GCAGCTTTAA ACCCAACCAG CAGACCACCC TCCTGCCTCA CAGCACCGCT 
GAACTCATGA GCAGCCTTAC AGGACTCATC AAAGTGCCAG GGATTGGTTA CAAGACACGT 
GAGAAGCTGA AGGCTCTGGG TTTGGTTAAT GTGAGAGATC TACAGTTGTA CCCTTTGTCC 
GATCTGGTGA AAGAGTTTGG AGAGATGAAC GCTAAGAGGA TCCAAAACCT CGCCTGTGGG 
ATTGATGATT CTCCAGTGAC TCCAGCGGGT CCTCCTCAGT CCCTCAGCGA TGAGGACTCA 
TTCAAGAAAA TGTCCACTCT AGAGGAAGTT CTAAAGAAAA TTGAAGAACT GCTCATCAAC 
CTTACTGAGA GGATGCACAA AGATGGCAGG CAGCCACACA CACTGAGACT AACGATTCGC 
AAATACACTG TCACCAATCG CTGGTTTAAT CGAGAGAGTC GTCAGTGTCC CATTCCCAAC 
CACACCGGGC TCAAGATCAC AGGAGGAAGC AGCGAGGCTG TGCCCCAGTT ACTGTCACTG 
TCGATGAAGC TCTTTCATAA AATGGTGGAC CCACATCAGC CTTTTCATCT GACTCTGCTC 
AATGTGTGTT TTAGCAACCT GCAGGCTAAA AGCTCCAGCA AAAGCTCCAT CGCTTCTTTT 
TTTACACACA AATCTCCTTC AACAACACAA ATCCCATCTC AGCAGGTGGA CTCTGATCTC 
TGTGAATCTG TTGCAACTTC CCATTTCACT CAAGCAGATG TTTTGCATAA AAATGATAAA 
GCGTGGAAAC CAGTAAAAAA CACATCAATA TCTCCAACCC AAACACAGTC GACCTCAGTC 
AACCCCAAAT CTTCCCTATC TCCCAGACAG CCTGTTTCAG AGCACTCACA ACATGCAAAC 
ATAAACATCA GACCGCCTTG TTTGAGCAGC GCTCCCAAAA AAACATTGCA TTTCAAGCTG 
CCTCCTAATG TTGATGCGGA TGTATTCAAA CTCCTTCCTG AACACATTCA GATGGAGCTG 
ATGTCCAGTT TTAAAAAGGG AGACTCCGTT TACAGACCCG CTGACACAGA AGATGTTGAC 
CCAGAACATC AACCCACGTT TGCCTCTCGC CAACCAAAAA CACCAAAACC TTGTGAGTCT 
GCAGTAAATG TCTGCACAAA TTCTGCTTCT AATCCCCATG AACTCAGCCA GAACTTTTCA 
TCTCCTCAGT CGATCCCGGA GTTCAGTTCT GAACTTGTCA TGGTCACAGA AAGTGACGAT 
ATCCCACACT CCACTCAGGC CATTCAGCCA GAAGTTCCTG CAAATGTGGA CCCTTATGTG 
TTTTCACAAC TTCCTGCTGA TGTGCAAAGG GAATTACTGA CCGAGTGGAG ACAGCAGAGG 
CCGGTCCTCA AAATCCCTTC AAAACACTCA CATAAATCCT CCAATAGCAG AGACAGGAAG 
ACCTCGACTA AAGGCAGCCA GTGTAACAAC ATACTGAAAT ACTTCAAACC CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299579.1
CDS	115..2091
Translation	MIRNPALRTKPLGIQQKYIIVTSNYVARELGVNKLMSVTDAVEKCPQLVLVKGEDLTHYREMSYKVTELLMSYCPLVERLGFDENFMDVTEMVEKRIKETRISDLSFNGHVYNHESVVVDEENIRLAVGSVIAAEMRQAIFSTLGLTGCAGVASNKLLAKLVSGSFKPNQQTTLLPHSTAELMSSLTGLIKVPGIGYKTREKLKALGLVNVRDLQLYPLSDLVKEFGEMNAKRIQNLACGIDDSPVTPAGPPQSLSDEDSFKKMSTLEEVLKKIEELLINLTERMHKDGRQPHTLRLTIRKYTVTNRWFNRESRQCPIPNHTGLKITGGSSEAVPQLLSLSMKLFHKMVDPHQPFHLTLLNVCFSNLQAKSSSKSSIASFFTHKSPSTTQIPSQQVDSDLCESVATSHFTQADVLHKNDKAWKPVKNTSISPTQTQSTSVNPKSSLSPRQPVSEHSQHANINIRPPCLSSAPKKTLHFKLPPNVDADVFKLLPEHIQMELMSSFKKGDSVYRPADTEDVDPEHQPTFASRQPKTPKPCESAVNVCTNSASNPHELSQNFSSPQSIPEFSSELVMVTESDDIPHSTQAIQPEVPANVDPYVFSQLPADVQRELLTEWRQQRPVLKIPSKHSHKSSNSRDRKTSTKGSQCNNILKYFKPN

Target ORF information:

RefSeq Version	XM_009301304.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301304.1

ORF Insert Sequence:

ATGATCCGAA ATCCAGCTCT CCGGACCAAA CCTCTTGGGA TACAGCAGAA ATATATTATT 
GTAACCAGTA ATTATGTGGC AAGAGAGCTT GGCGTGAACA AGCTGATGTC AGTGACAGAT 
GCAGTGGAGA AATGTCCACA GCTGGTGCTT GTAAAAGGAG AAGATCTCAC TCATTACAGA 
GAAATGTCCT ACAAAGTCAC TGAATTGCTG ATGTCGTACT GCCCTCTAGT GGAGCGGCTG 
GGCTTTGATG AGAACTTCAT GGACGTTACA GAAATGGTGG AGAAACGAAT AAAGGAGACC 
CGTATTTCAG ATCTCTCCTT CAATGGACAC GTATACAATC ATGAAAGTGT TGTGGTTGAT 
GAGGAGAACA TTCGTTTGGC TGTCGGCTCT GTGATTGCCG CTGAGATGAG GCAAGCCATC 
TTCAGCACAC TGGGGCTAAC TGGCTGTGCT GGTGTCGCTA GCAACAAACT ATTAGCCAAG 
CTGGTGTCAG GCAGCTTTAA ACCCAACCAG CAGACCACCC TCCTGCCTCA CAGCACCGCT 
GAACTCATGA GCAGCCTTAC AGGACTCATC AAAGTGCCAG GGATTGGTTA CAAGACACGT 
GAGAAGCTGA AGGCTCTGGG TTTGGTTAAT GTGAGAGATC TACAGTTGTA CCCTTTGTCC 
GATCTGGTGA AAGAGTTTGG AGAGATGAAC GCTAAGAGGA TCCAAAACCT CGCCTGTGGG 
ATTGATGATT CTCCAGTGAC TCCAGCGGGT CCTCCTCAGT CCCTCAGCGA TGAGGACTCA 
TTCAAGAAAA TGTCCACTCT AGAGGAAGTT CTAAAGAAAA TTGAAGAACT GCTCATCAAC 
CTTACTGAGA GGATGCACAA AGATGGCAGG CAGCCACACA CACTGAGACT AACGATTCGC 
AAATACACTG TCACCAATCG CTGGTTTAAT CGAGAGAGTC GTCAGTGTCC CATTCCCAAC 
CACACCGGGC TCAAGATCAC AGGAGGAAGC AGCGAGGCTG TGCCCCAGTT ACTGTCACTG 
TCGATGAAGC TCTTTCATAA AATGGTGGAC CCACATCAGC CTTTTCATCT GACTCTGCTC 
AATGTGTGTT TTAGCAACCT GCAGGCTAAA AGCTCCAGCA AAAGCTCCAT CGCTTCTTTT 
TTTACACACA AATCTCCTTC AACAACACAA ATCCCATCTC AGCAGGTGGA CTCTGATCTC 
TGTGAATCTG TTGCAACTTC CCATTTCACT CAAGCAGATG TTTTGCATAA AAATGATAAA 
GCGTGGAAAC CAGTAAAAAA CACATCAATA TCTCCAACCC AAACACAGTC GACCTCAGTC 
AACCCCAAAT CTTCCCTATC TCCCAGACAG CCTGTTTCAG AGCACTCACA ACATGCAAAC 
ATAAACATCA GACCGCCTTG TTTGAGCAGC GCTCCCAAAA AAACATTGCA TTTCAAGCTG 
CCTCCTAATG TTGATGCGGA TGTATTCAAA CTCCTTCCTG AACACATTCA GATGGAGCTG 
ATGTCCAGTT TTAAAAAGGG AGACTCCGTT TACAGACCCG CTGACACAGA AGATGTTGAC 
CCAGAACATC AACCCACGTT TGCCTCTCGC CAACCAAAAA CACCAAAACC TTGTGAGTCT 
GCAGTAAATG TCTGCACAAA TTCTGCTTCT AATCCCCATG AACTCAGCCA GAACTTTTCA 
TCTCCTCAGT CGATCCCGGA GTTCAGTTCT GAACTTGTCA TGGTCACAGA AAGTGACGAT 
ATCCCACACT CCACTCAGGC CATTCAGCCA GAAGTTCCTG CAAATGTGGA CCCTTATGTG 
TTTTCACAAC TTCCTGCTGA TGTGCAAAGG GAATTACTGA CCGAGTGGAG ACAGCAGAGG 
CCGGTCCTCA AAATCCCTTC AAAACACTCA CATAAATCCT CCAATAGCAG AGACAGGAAG 
ACCTCGACTA AAGGCAGCCA GTGTAACAAC ATACTGAAAT ACTTCAAACC CAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poli ( NM_001017834.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017834.1 Danio rerio polymerase (DNA directed) iota (poli), mRNA.

Price & Availability↑

CloneID	ODa32650
Clone ID Related Accession (Same CDS sequence)	NM_001017834.1
Accession Version	NM_001017834.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DNA polymerase iota
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092781.1. ##Evidence-Data-START## Transcript exon combination :: BC092781.1, BC165649.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG AAGGAAGTTC CACTGATAGA GTTTCATTGA CATCCCAGAG AGTGATCCTG 
CATTTTGACC TGGACTGTTT TTATGCACAA GTTGAGATGA TCCGAAATCC AGCCCTCCGG 
ACCAAACCTC TTGGGATACA GCAGAAATAT ATTATTGTAA CCAGTAATTA TGTGGCAAGA 
GAGCTTGGCG TGAACAAACT GATGTCAGTG ACAGATGCAG TGGAGAAATG TCCACAGCTG 
GTGCTTGTAA AAGGAGAAGA TCTCACTCAT TACAGAGAAA TGTCCTACAA AGTCACTGAA 
TTGCTGATGT CGTACTGCCC TCTAGTGGAG CGGCTGGGCT TTGATGAGAA CTTCATGGAC 
GTTACAGAAA TGGTGGAGAA ACGAATAAAG GAGACCCGTA TTTCAGATCT CTCCTTCAAT 
GGACACGTAT ACAATCATGA AAGTGTTGTG GTTGATGAGG AGAACATTCG TTTGGCTGTC 
GGCTCTGTGA TTGCCGCTGA GATGAGGCAA GCCATCTTCA GCACACTGGG GCTAACTGGC 
TGTGCTGGTG TCGCTAGCAA CAAACTATTA GCCAAGCTGG TGTCAGGCAG CTTTAAACCC 
AACCAGCAGA CCACCCTCCT GCCTCACAGC AACGCTGAAC TCATGAGCAG CCTTACAGGA 
CTCATCAAAG TGCCAGGGAT TGGTTACAAG ACACGTGAGA AGCTGAAGGC TCTGGGTTTG 
GTTAATGTGA GAGATCTACA GTTGTACCCT TTGTCCGATC TGGTGAAAGA GTTTGGAGAG 
ATGAACGCTA AGAGGGTCCA AAACCTCGCC TGTGGGATTG ATGACTCTCC AGTGACTCCA 
GCGGGTCCTC CTCAGTCCCT CAGCGATGAG GACTCATTCA AGAAAATGTC CACTCTAGAG 
GAAGTTCTAA AGAAAATTGA AGAACTGCTC ATCAACCTTA CTGAGAGGAT GCACAAAGAT 
GGCAGGCAGC CACACACACT GAGACTAACG ATTCGCAAAT ACACTGTCAC CAATCGCTGG 
TTTAATCGAG AGAGTCGTCA GTGTCCCATT CCCAACCACA CCGGGCTCAA GATCACAGGA 
GGAAGCAGCG AGGCTGTGCC CCAGTTACTG TCACTGTCGA TGAAGCTCTT TCATAAAATG 
GTGGACCCAC ATCAGCCTTT TCATCTGACT CTGCTCAATG TGTGTTTTAG CAACCTGCAG 
GCTAAAAGCT CCAGCAAAAG CTCCATCGCT TCTTTTTTTA CACACAAATC TCCTTCAACA 
ACACAAATCC CATCTCAGCA GGTGGACTCT GATCTCTGTG AATCTGTTGC ATCTTCCCAT 
TTCACTCAAG CAGATGTTTT GCATAAAAAT GATAAAGCGT GGAAACCAGT AAACAACACA 
TCAATATCTC CAACCCAAAC ACAGTCGACC TCAGTCAACC CCAAATCTTC CCTATCTCCC 
AGACAGCCTG TTTCAGAGCA CTCACAACAT GCAAACATAA ACATCAGACC GCCTTGTTTG 
AGCAGCGCTC CCAAAACAAC ATTGCATTTC AAGCTGCCTC CTAATGTTGA TGCGGATGTA 
TTCAAACTCC TTCCTGAACA CATTCAGATG GAGCTGATGT CCAGTTTTAA AAAGGGAGAC 
TCCTTTTACA GACCCGCTGA CACAGAAGAT GTTGACCCAG AACATCAACC CACGTTTGCC 
TCTCGCCAAC CAAAAACACC AAAACCTTGT GAGTCTGCAG TAAATGTCTG CACAAATTCT 
GCTTCTAATC CCCATGAACT CAGCCAGAAC TTTTCATCTC CTCAGTCGAT CCCGGAGTTC 
AGTTCTGAAC TTGTCATGGT CACAGAAAGT GACGATATCC CACACTCCAC TCAGGCCATT 
CAGCCAGAAG TTCCTGCAAA TGTGGACCCT TATGTGTTTT CACAACTTCC TGCTGATGTG 
CAAAGGGAAT TACTGACCGA GTGGAGACAG CAGAGGCCGG TCCTCAAAAT CCCTTCAAAA 
CACTCACATA AATCCTCCAA TAGCAGAGAC AGGAAGACCT CGACTAAAGG CAGCCAGTGT 
AACAACATAC TGAAATACTT CAAACCCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017834.1
CDS	119..2251
Translation	MDSDEEEDNNDWERSLESDILETEGSSTDRVSLTSQRVILHFDLDCFYAQVEMIRNPALRTKPLGIQQKYIIVTSNYVARELGVNKLMSVTDAVEKCPQLVLVKGEDLTHYREMSYKVTELLMSYCPLVERLGFDENFMDVTEMVEKRIKETRISDLSFNGHVYNHESVVVDEENIRLAVGSVIAAEMRQAIFSTLGLTGCAGVASNKLLAKLVSGSFKPNQQTTLLPHSNAELMSSLTGLIKVPGIGYKTREKLKALGLVNVRDLQLYPLSDLVKEFGEMNAKRVQNLACGIDDSPVTPAGPPQSLSDEDSFKKMSTLEEVLKKIEELLINLTERMHKDGRQPHTLRLTIRKYTVTNRWFNRESRQCPIPNHTGLKITGGSSEAVPQLLSLSMKLFHKMVDPHQPFHLTLLNVCFSNLQAKSSSKSSIASFFTHKSPSTTQIPSQQVDSDLCESVASSHFTQADVLHKNDKAWKPVNNTSISPTQTQSTSVNPKSSLSPRQPVSEHSQHANINIRPPCLSSAPKTTLHFKLPPNVDADVFKLLPEHIQMELMSSFKKGDSFYRPADTEDVDPEHQPTFASRQPKTPKPCESAVNVCTNSASNPHELSQNFSSPQSIPEFSSELVMVTESDDIPHSTQAIQPEVPANVDPYVFSQLPADVQRELLTEWRQQRPVLKIPSKHSHKSSNSRDRKTSTKGSQCNNILKYFKPN

Target ORF information:

RefSeq Version	NM_001017834.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed) iota (poli), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017834.1

ORF Insert Sequence:

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG AAGGAAGTTC CACTGATAGA GTTTCATTGA CATCCCAGAG AGTGATCCTG 
CATTTTGACC TGGACTGTTT TTATGCACAA GTTGAGATGA TCCGAAATCC AGCCCTCCGG 
ACCAAACCTC TTGGGATACA GCAGAAATAT ATTATTGTAA CCAGTAATTA TGTGGCAAGA 
GAGCTTGGCG TGAACAAACT GATGTCAGTG ACAGATGCAG TGGAGAAATG TCCACAGCTG 
GTGCTTGTAA AAGGAGAAGA TCTCACTCAT TACAGAGAAA TGTCCTACAA AGTCACTGAA 
TTGCTGATGT CGTACTGCCC TCTAGTGGAG CGGCTGGGCT TTGATGAGAA CTTCATGGAC 
GTTACAGAAA TGGTGGAGAA ACGAATAAAG GAGACCCGTA TTTCAGATCT CTCCTTCAAT 
GGACACGTAT ACAATCATGA AAGTGTTGTG GTTGATGAGG AGAACATTCG TTTGGCTGTC 
GGCTCTGTGA TTGCCGCTGA GATGAGGCAA GCCATCTTCA GCACACTGGG GCTAACTGGC 
TGTGCTGGTG TCGCTAGCAA CAAACTATTA GCCAAGCTGG TGTCAGGCAG CTTTAAACCC 
AACCAGCAGA CCACCCTCCT GCCTCACAGC AACGCTGAAC TCATGAGCAG CCTTACAGGA 
CTCATCAAAG TGCCAGGGAT TGGTTACAAG ACACGTGAGA AGCTGAAGGC TCTGGGTTTG 
GTTAATGTGA GAGATCTACA GTTGTACCCT TTGTCCGATC TGGTGAAAGA GTTTGGAGAG 
ATGAACGCTA AGAGGGTCCA AAACCTCGCC TGTGGGATTG ATGACTCTCC AGTGACTCCA 
GCGGGTCCTC CTCAGTCCCT CAGCGATGAG GACTCATTCA AGAAAATGTC CACTCTAGAG 
GAAGTTCTAA AGAAAATTGA AGAACTGCTC ATCAACCTTA CTGAGAGGAT GCACAAAGAT 
GGCAGGCAGC CACACACACT GAGACTAACG ATTCGCAAAT ACACTGTCAC CAATCGCTGG 
TTTAATCGAG AGAGTCGTCA GTGTCCCATT CCCAACCACA CCGGGCTCAA GATCACAGGA 
GGAAGCAGCG AGGCTGTGCC CCAGTTACTG TCACTGTCGA TGAAGCTCTT TCATAAAATG 
GTGGACCCAC ATCAGCCTTT TCATCTGACT CTGCTCAATG TGTGTTTTAG CAACCTGCAG 
GCTAAAAGCT CCAGCAAAAG CTCCATCGCT TCTTTTTTTA CACACAAATC TCCTTCAACA 
ACACAAATCC CATCTCAGCA GGTGGACTCT GATCTCTGTG AATCTGTTGC ATCTTCCCAT 
TTCACTCAAG CAGATGTTTT GCATAAAAAT GATAAAGCGT GGAAACCAGT AAACAACACA 
TCAATATCTC CAACCCAAAC ACAGTCGACC TCAGTCAACC CCAAATCTTC CCTATCTCCC 
AGACAGCCTG TTTCAGAGCA CTCACAACAT GCAAACATAA ACATCAGACC GCCTTGTTTG 
AGCAGCGCTC CCAAAACAAC ATTGCATTTC AAGCTGCCTC CTAATGTTGA TGCGGATGTA 
TTCAAACTCC TTCCTGAACA CATTCAGATG GAGCTGATGT CCAGTTTTAA AAAGGGAGAC 
TCCTTTTACA GACCCGCTGA CACAGAAGAT GTTGACCCAG AACATCAACC CACGTTTGCC 
TCTCGCCAAC CAAAAACACC AAAACCTTGT GAGTCTGCAG TAAATGTCTG CACAAATTCT 
GCTTCTAATC CCCATGAACT CAGCCAGAAC TTTTCATCTC CTCAGTCGAT CCCGGAGTTC 
AGTTCTGAAC TTGTCATGGT CACAGAAAGT GACGATATCC CACACTCCAC TCAGGCCATT 
CAGCCAGAAG TTCCTGCAAA TGTGGACCCT TATGTGTTTT CACAACTTCC TGCTGATGTG 
CAAAGGGAAT TACTGACCGA GTGGAGACAG CAGAGGCCGG TCCTCAAAAT CCCTTCAAAA 
CACTCACATA AATCCTCCAA TAGCAGAGAC AGGAAGACCT CGACTAAAGG CAGCCAGTGT 
AACAACATAC TGAAATACTT CAAACCCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poli ( XM_017356083.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211572.1 Danio rerio polymerase (DNA directed) iota (poli), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59079
Clone ID Related Accession (Same CDS sequence)	XM_017356083.1
Accession Version	XM_017356083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase iota isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG AAGGAAGTTC CACTGATAGA GTTTCATTGA CATCCCAGAG AGTGATCCTG 
CATTTTGACC TGGACTGTTT TTATGCACAA GTTGAGATGA TCCGAAATCC AGCTCTCCGG 
ACCAAACCTC TTGGGATACA GCAGAAATAT ATTATTGTAA CCAGTAATTA TGTGGCAAGA 
GAGCTTGGCG TGAACAAGCT GATGTCAGTG ACAGATGCAG TGGAGAAATG TCCACAGCTG 
GTGCTTGTAA AAGGAGAAGA TCTCACTCAT TACAGAGAAA TGTCCTACAA AGTCACTGAA 
TTGCTGATGT CGTACTGCCC TCTAGTGGAG CGGCTGGGCT TTGATGAGAA CTTCATGGAC 
GTTACAGAAA TGGTGGAGAA ACGAATAAAG GAGACCCGTA TTTCAGATCT CTCCTTCAAT 
GGACACGTAT ACAATCATGA AAGTGTTGTG GTTGATGAGG AGAACATTCG TTTGGCTGTC 
GGCTCTGTGA TTGCCGCTGA GATGAGGCAA GCCATCTTCA GCACACTGGG GCTAACTGGC 
TGTGCTGGTG TCGCTAGCAA CAAACTATTA GCCAAGCTGG TGTCAGGCAG CTTTAAACCC 
AACCAGCAGA CCACCCTCCT GCCTCACAGC ACCGCTGAAC TCATGAGCAG CCTTACAGGA 
CTCATCAAAG TGCCAGGGAT TGGTTACAAG ACACGTGAGA AGCTGAAGGC TCTGGGTTTG 
GTTAATGTGA GAGATCTACA GTTGTACCCT TTGTCCGATC TGGTGAAAGA GTTTGGAGAG 
ATGAACGCTA AGAGGATCCA AAACCTCGCC TGTGGGATTG ATGATTCTCC AGTGACTCCA 
GCGGGTCCTC CTCAGTCCCT CAGCGATGAG GACTCATTCA AGAAAATGTC CACTCTAGAG 
GAAGTTCTAA AGAAAATTGA AGAACTGCTC ATCAACCTTA CTGAGAGGAT GCACAAAGAT 
GGCAGGCAGC CACACACACT GAGACTAACG ATTCGCAAAT ACACTGTCAC CAATCGCTGG 
TTTAATCGAG AGAGTCGTCA GTGTCCCATT CCCAACCACA CCGGGCTCAA GATCACAGGA 
GGAAGCAGCG AGGCTGTGCC CCAGTTACTG TCACTGTCGA TGAAGCTCTT TCATAAAATG 
GTGGACCCAC ATCAGCCTTT TCATCTGACT CTGCTCAATG TGTGTTTTAG CAACCTGCAG 
GCTAAAAGCT CCAGCAAAAG CTCCATCGCT TCTTTTTTTA CACACAAATC TCCTTCAACA 
ACACAAATCC CATCTCAGCA GGTGGACTCT GATCTCTGTG AATCTGTTGC AACTTCCCAT 
TTCACTCAAG CAGATGTTTT GCATAAAAAT GATAAAGCGT GGAAACCAGT AAAAAACACA 
TCAATATCTC CAACCCAAAC ACAGTCGACC TCAGTCAACC CCAAATCTTC CCTATCTCCC 
AGACAGCCTG TTTCAGAGCA CTCACAACAT GCAAACATAA ACATCAGACC GCCTTGTTTG 
AGCAGCGCTC CCAAAAAAAC ATTGCATTTC AAGCTGCCTC CTAATGTTGA TGCGGATGTA 
TTCAAACTCC TTCCTGAACA CATTCAGATG GAGCTGATGT CCAGTTTTAA AAAGGGAGAC 
TCCGTTTACA GACCCGCTGA CACAGAAGAT GTTGACCCAG AACATCAACC CACGTTTGCC 
TCTCGCCAAC CAAAAACACC AAAACCTTGT GAGTCTGCAG TAAATGTCTG CACAAATTCT 
GCTTCTAATC CCCATGAACT CAGCCAGAAC TTTTCATCTC CTCAGTCGAT CCCGGAGTTC 
AGTTCTGAAC TTGTCATGGT CACAGAAAGT GACGATATCC CACACTCCAC TCAGGCCATT 
CAGCCAGAAG TTCCTGCAAA TGTGGACCCT TATGTGTTTT CACAACTTCC TGCTGATGTG 
CAAAGGGAAT TACTGACCGA GTGGAGACAG CAGAGGCCGG TCCTCAAAAT CCCTTCAAAA 
CACTCACATA AATCCTCCAA TAGCAGAGAC AGGAAGACCT CGACTAAAGG CAGCCAGTGT 
AACAACATAC TGAAATACTT CAAACCCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211572.1
CDS	19..2151
Translation	MDSDEEEDNNDWERSLESDILETEGSSTDRVSLTSQRVILHFDLDCFYAQVEMIRNPALRTKPLGIQQKYIIVTSNYVARELGVNKLMSVTDAVEKCPQLVLVKGEDLTHYREMSYKVTELLMSYCPLVERLGFDENFMDVTEMVEKRIKETRISDLSFNGHVYNHESVVVDEENIRLAVGSVIAAEMRQAIFSTLGLTGCAGVASNKLLAKLVSGSFKPNQQTTLLPHSTAELMSSLTGLIKVPGIGYKTREKLKALGLVNVRDLQLYPLSDLVKEFGEMNAKRIQNLACGIDDSPVTPAGPPQSLSDEDSFKKMSTLEEVLKKIEELLINLTERMHKDGRQPHTLRLTIRKYTVTNRWFNRESRQCPIPNHTGLKITGGSSEAVPQLLSLSMKLFHKMVDPHQPFHLTLLNVCFSNLQAKSSSKSSIASFFTHKSPSTTQIPSQQVDSDLCESVATSHFTQADVLHKNDKAWKPVKNTSISPTQTQSTSVNPKSSLSPRQPVSEHSQHANINIRPPCLSSAPKKTLHFKLPPNVDADVFKLLPEHIQMELMSSFKKGDSVYRPADTEDVDPEHQPTFASRQPKTPKPCESAVNVCTNSASNPHELSQNFSSPQSIPEFSSELVMVTESDDIPHSTQAIQPEVPANVDPYVFSQLPADVQRELLTEWRQQRPVLKIPSKHSHKSSNSRDRKTSTKGSQCNNILKYFKPN

Target ORF information:

RefSeq Version	XM_017356083.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356083.1

ORF Insert Sequence:

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG AAGGAAGTTC CACTGATAGA GTTTCATTGA CATCCCAGAG AGTGATCCTG 
CATTTTGACC TGGACTGTTT TTATGCACAA GTTGAGATGA TCCGAAATCC AGCTCTCCGG 
ACCAAACCTC TTGGGATACA GCAGAAATAT ATTATTGTAA CCAGTAATTA TGTGGCAAGA 
GAGCTTGGCG TGAACAAGCT GATGTCAGTG ACAGATGCAG TGGAGAAATG TCCACAGCTG 
GTGCTTGTAA AAGGAGAAGA TCTCACTCAT TACAGAGAAA TGTCCTACAA AGTCACTGAA 
TTGCTGATGT CGTACTGCCC TCTAGTGGAG CGGCTGGGCT TTGATGAGAA CTTCATGGAC 
GTTACAGAAA TGGTGGAGAA ACGAATAAAG GAGACCCGTA TTTCAGATCT CTCCTTCAAT 
GGACACGTAT ACAATCATGA AAGTGTTGTG GTTGATGAGG AGAACATTCG TTTGGCTGTC 
GGCTCTGTGA TTGCCGCTGA GATGAGGCAA GCCATCTTCA GCACACTGGG GCTAACTGGC 
TGTGCTGGTG TCGCTAGCAA CAAACTATTA GCCAAGCTGG TGTCAGGCAG CTTTAAACCC 
AACCAGCAGA CCACCCTCCT GCCTCACAGC ACCGCTGAAC TCATGAGCAG CCTTACAGGA 
CTCATCAAAG TGCCAGGGAT TGGTTACAAG ACACGTGAGA AGCTGAAGGC TCTGGGTTTG 
GTTAATGTGA GAGATCTACA GTTGTACCCT TTGTCCGATC TGGTGAAAGA GTTTGGAGAG 
ATGAACGCTA AGAGGATCCA AAACCTCGCC TGTGGGATTG ATGATTCTCC AGTGACTCCA 
GCGGGTCCTC CTCAGTCCCT CAGCGATGAG GACTCATTCA AGAAAATGTC CACTCTAGAG 
GAAGTTCTAA AGAAAATTGA AGAACTGCTC ATCAACCTTA CTGAGAGGAT GCACAAAGAT 
GGCAGGCAGC CACACACACT GAGACTAACG ATTCGCAAAT ACACTGTCAC CAATCGCTGG 
TTTAATCGAG AGAGTCGTCA GTGTCCCATT CCCAACCACA CCGGGCTCAA GATCACAGGA 
GGAAGCAGCG AGGCTGTGCC CCAGTTACTG TCACTGTCGA TGAAGCTCTT TCATAAAATG 
GTGGACCCAC ATCAGCCTTT TCATCTGACT CTGCTCAATG TGTGTTTTAG CAACCTGCAG 
GCTAAAAGCT CCAGCAAAAG CTCCATCGCT TCTTTTTTTA CACACAAATC TCCTTCAACA 
ACACAAATCC CATCTCAGCA GGTGGACTCT GATCTCTGTG AATCTGTTGC AACTTCCCAT 
TTCACTCAAG CAGATGTTTT GCATAAAAAT GATAAAGCGT GGAAACCAGT AAAAAACACA 
TCAATATCTC CAACCCAAAC ACAGTCGACC TCAGTCAACC CCAAATCTTC CCTATCTCCC 
AGACAGCCTG TTTCAGAGCA CTCACAACAT GCAAACATAA ACATCAGACC GCCTTGTTTG 
AGCAGCGCTC CCAAAAAAAC ATTGCATTTC AAGCTGCCTC CTAATGTTGA TGCGGATGTA 
TTCAAACTCC TTCCTGAACA CATTCAGATG GAGCTGATGT CCAGTTTTAA AAAGGGAGAC 
TCCGTTTACA GACCCGCTGA CACAGAAGAT GTTGACCCAG AACATCAACC CACGTTTGCC 
TCTCGCCAAC CAAAAACACC AAAACCTTGT GAGTCTGCAG TAAATGTCTG CACAAATTCT 
GCTTCTAATC CCCATGAACT CAGCCAGAAC TTTTCATCTC CTCAGTCGAT CCCGGAGTTC 
AGTTCTGAAC TTGTCATGGT CACAGAAAGT GACGATATCC CACACTCCAC TCAGGCCATT 
CAGCCAGAAG TTCCTGCAAA TGTGGACCCT TATGTGTTTT CACAACTTCC TGCTGATGTG 
CAAAGGGAAT TACTGACCGA GTGGAGACAG CAGAGGCCGG TCCTCAAAAT CCCTTCAAAA 
CACTCACATA AATCCTCCAA TAGCAGAGAC AGGAAGACCT CGACTAAAGG CAGCCAGTGT 
AACAACATAC TGAAATACTT CAAACCCAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poli ( XM_017356084.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211573.1 Danio rerio polymerase (DNA directed) iota (poli), transcript variant X1, mRNA.

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CloneID	ODa59080
Clone ID Related Accession (Same CDS sequence)	XM_017356084.2 , XM_017356084.1
Accession Version	XM_017356084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA polymerase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356084.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG GAAGTTCCAC TGATAGAGTT TCATTGACAT CCCAGAGAGT GATCCTGCAT 
TTTGACCTGG ACTGTTTTTA TGCACAAGTT GAGATGATCC GAAATCCAGC TCTCCGGACC 
AAACCTCTTG GGATACAGCA GAAATATATT ATTGTAACCA GTAATTATGT GGCAAGAGAG 
CTTGGCGTGA ACAAGCTGAT GTCAGTGACA GATGCAGTGG AGAAATGTCC ACAGCTGGTG 
CTTGTAAAAG GAGAAGATCT CACTCATTAC AGAGAAATGT CCTACAAAGT CACTGAATTG 
CTGATGTCGT ACTGCCCTCT AGTGGAGCGG CTGGGCTTTG ATGAGAACTT CATGGACGTT 
ACAGAAATGG TGGAGAAACG AATAAAGGAG ACCCGTATTT CAGATCTCTC CTTCAATGGA 
CACGTATACA ATCATGAAAG TGTTGTGGTT GATGAGGAGA ACATTCGTTT GGCTGTCGGC 
TCTGTGATTG CCGCTGAGAT GAGGCAAGCC ATCTTCAGCA CACTGGGGCT AACTGGCTGT 
GCTGGTGTCG CTAGCAACAA ACTATTAGCC AAGCTGGTGT CAGGCAGCTT TAAACCCAAC 
CAGCAGACCA CCCTCCTGCC TCACAGCACC GCTGAACTCA TGAGCAGCCT TACAGGACTC 
ATCAAAGTGC CAGGGATTGG TTACAAGACA CGTGAGAAGC TGAAGGCTCT GGGTTTGGTT 
AATGTGAGAG ATCTACAGTT GTACCCTTTG TCCGATCTGG TGAAAGAGTT TGGAGAGATG 
AACGCTAAGA GGATCCAAAA CCTCGCCTGT GGGATTGATG ATTCTCCAGT GACTCCAGCG 
GGTCCTCCTC AGTCCCTCAG CGATGAGGAC TCATTCAAGA AAATGTCCAC TCTAGAGGAA 
GTTCTAAAGA AAATTGAAGA ACTGCTCATC AACCTTACTG AGAGGATGCA CAAAGATGGC 
AGGCAGCCAC ACACACTGAG ACTAACGATT CGCAAATACA CTGTCACCAA TCGCTGGTTT 
AATCGAGAGA GTCGTCAGTG TCCCATTCCC AACCACACCG GGCTCAAGAT CACAGGAGGA 
AGCAGCGAGG CTGTGCCCCA GTTACTGTCA CTGTCGATGA AGCTCTTTCA TAAAATGGTG 
GACCCACATC AGCCTTTTCA TCTGACTCTG CTCAATGTGT GTTTTAGCAA CCTGCAGGCT 
AAAAGCTCCA GCAAAAGCTC CATCGCTTCT TTTTTTACAC ACAAATCTCC TTCAACAACA 
CAAATCCCAT CTCAGCAGGT GGACTCTGAT CTCTGTGAAT CTGTTGCAAC TTCCCATTTC 
ACTCAAGCAG ATGTTTTGCA TAAAAATGAT AAAGCGTGGA AACCAGTAAA AAACACATCA 
ATATCTCCAA CCCAAACACA GTCGACCTCA GTCAACCCCA AATCTTCCCT ATCTCCCAGA 
CAGCCTGTTT CAGAGCACTC ACAACATGCA AACATAAACA TCAGACCGCC TTGTTTGAGC 
AGCGCTCCCA AAAAAACATT GCATTTCAAG CTGCCTCCTA ATGTTGATGC GGATGTATTC 
AAACTCCTTC CTGAACACAT TCAGATGGAG CTGATGTCCA GTTTTAAAAA GGGAGACTCC 
GTTTACAGAC CCGCTGACAC AGAAGATGTT GACCCAGAAC ATCAACCCAC GTTTGCCTCT 
CGCCAACCAA AAACACCAAA ACCTTGTGAG TCTGCAGTAA ATGTCTGCAC AAATTCTGCT 
TCTAATCCCC ATGAACTCAG CCAGAACTTT TCATCTCCTC AGTCGATCCC GGAGTTCAGT 
TCTGAACTTG TCATGGTCAC AGAAAGTGAC GATATCCCAC ACTCCACTCA GGCCATTCAG 
CCAGAAGTTC CTGCAAATGT GGACCCTTAT GTGTTTTCAC AACTTCCTGC TGATGTGCAA 
AGGGAATTAC TGACCGAGTG GAGACAGCAG AGGCCGGTCC TCAAAATCCC TTCAAAACAC 
TCACATAAAT CCTCCAATAG CAGAGACAGG AAGACCTCGA CTAAAGGCAG CCAGTGTAAC 
AACATACTGA AATACTTCAA ACCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211573.1
CDS	127..2256
Translation	MDSDEEEDNNDWERSLESDILETGSSTDRVSLTSQRVILHFDLDCFYAQVEMIRNPALRTKPLGIQQKYIIVTSNYVARELGVNKLMSVTDAVEKCPQLVLVKGEDLTHYREMSYKVTELLMSYCPLVERLGFDENFMDVTEMVEKRIKETRISDLSFNGHVYNHESVVVDEENIRLAVGSVIAAEMRQAIFSTLGLTGCAGVASNKLLAKLVSGSFKPNQQTTLLPHSTAELMSSLTGLIKVPGIGYKTREKLKALGLVNVRDLQLYPLSDLVKEFGEMNAKRIQNLACGIDDSPVTPAGPPQSLSDEDSFKKMSTLEEVLKKIEELLINLTERMHKDGRQPHTLRLTIRKYTVTNRWFNRESRQCPIPNHTGLKITGGSSEAVPQLLSLSMKLFHKMVDPHQPFHLTLLNVCFSNLQAKSSSKSSIASFFTHKSPSTTQIPSQQVDSDLCESVATSHFTQADVLHKNDKAWKPVKNTSISPTQTQSTSVNPKSSLSPRQPVSEHSQHANINIRPPCLSSAPKKTLHFKLPPNVDADVFKLLPEHIQMELMSSFKKGDSVYRPADTEDVDPEHQPTFASRQPKTPKPCESAVNVCTNSASNPHELSQNFSSPQSIPEFSSELVMVTESDDIPHSTQAIQPEVPANVDPYVFSQLPADVQRELLTEWRQQRPVLKIPSKHSHKSSNSRDRKTSTKGSQCNNILKYFKPN

Target ORF information:

RefSeq Version	XM_017356084.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356084.2

ORF Insert Sequence:

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG GAAGTTCCAC TGATAGAGTT TCATTGACAT CCCAGAGAGT GATCCTGCAT 
TTTGACCTGG ACTGTTTTTA TGCACAAGTT GAGATGATCC GAAATCCAGC TCTCCGGACC 
AAACCTCTTG GGATACAGCA GAAATATATT ATTGTAACCA GTAATTATGT GGCAAGAGAG 
CTTGGCGTGA ACAAGCTGAT GTCAGTGACA GATGCAGTGG AGAAATGTCC ACAGCTGGTG 
CTTGTAAAAG GAGAAGATCT CACTCATTAC AGAGAAATGT CCTACAAAGT CACTGAATTG 
CTGATGTCGT ACTGCCCTCT AGTGGAGCGG CTGGGCTTTG ATGAGAACTT CATGGACGTT 
ACAGAAATGG TGGAGAAACG AATAAAGGAG ACCCGTATTT CAGATCTCTC CTTCAATGGA 
CACGTATACA ATCATGAAAG TGTTGTGGTT GATGAGGAGA ACATTCGTTT GGCTGTCGGC 
TCTGTGATTG CCGCTGAGAT GAGGCAAGCC ATCTTCAGCA CACTGGGGCT AACTGGCTGT 
GCTGGTGTCG CTAGCAACAA ACTATTAGCC AAGCTGGTGT CAGGCAGCTT TAAACCCAAC 
CAGCAGACCA CCCTCCTGCC TCACAGCACC GCTGAACTCA TGAGCAGCCT TACAGGACTC 
ATCAAAGTGC CAGGGATTGG TTACAAGACA CGTGAGAAGC TGAAGGCTCT GGGTTTGGTT 
AATGTGAGAG ATCTACAGTT GTACCCTTTG TCCGATCTGG TGAAAGAGTT TGGAGAGATG 
AACGCTAAGA GGATCCAAAA CCTCGCCTGT GGGATTGATG ATTCTCCAGT GACTCCAGCG 
GGTCCTCCTC AGTCCCTCAG CGATGAGGAC TCATTCAAGA AAATGTCCAC TCTAGAGGAA 
GTTCTAAAGA AAATTGAAGA ACTGCTCATC AACCTTACTG AGAGGATGCA CAAAGATGGC 
AGGCAGCCAC ACACACTGAG ACTAACGATT CGCAAATACA CTGTCACCAA TCGCTGGTTT 
AATCGAGAGA GTCGTCAGTG TCCCATTCCC AACCACACCG GGCTCAAGAT CACAGGAGGA 
AGCAGCGAGG CTGTGCCCCA GTTACTGTCA CTGTCGATGA AGCTCTTTCA TAAAATGGTG 
GACCCACATC AGCCTTTTCA TCTGACTCTG CTCAATGTGT GTTTTAGCAA CCTGCAGGCT 
AAAAGCTCCA GCAAAAGCTC CATCGCTTCT TTTTTTACAC ACAAATCTCC TTCAACAACA 
CAAATCCCAT CTCAGCAGGT GGACTCTGAT CTCTGTGAAT CTGTTGCAAC TTCCCATTTC 
ACTCAAGCAG ATGTTTTGCA TAAAAATGAT AAAGCGTGGA AACCAGTAAA AAACACATCA 
ATATCTCCAA CCCAAACACA GTCGACCTCA GTCAACCCCA AATCTTCCCT ATCTCCCAGA 
CAGCCTGTTT CAGAGCACTC ACAACATGCA AACATAAACA TCAGACCGCC TTGTTTGAGC 
AGCGCTCCCA AAAAAACATT GCATTTCAAG CTGCCTCCTA ATGTTGATGC GGATGTATTC 
AAACTCCTTC CTGAACACAT TCAGATGGAG CTGATGTCCA GTTTTAAAAA GGGAGACTCC 
GTTTACAGAC CCGCTGACAC AGAAGATGTT GACCCAGAAC ATCAACCCAC GTTTGCCTCT 
CGCCAACCAA AAACACCAAA ACCTTGTGAG TCTGCAGTAA ATGTCTGCAC AAATTCTGCT 
TCTAATCCCC ATGAACTCAG CCAGAACTTT TCATCTCCTC AGTCGATCCC GGAGTTCAGT 
TCTGAACTTG TCATGGTCAC AGAAAGTGAC GATATCCCAC ACTCCACTCA GGCCATTCAG 
CCAGAAGTTC CTGCAAATGT GGACCCTTAT GTGTTTTCAC AACTTCCTGC TGATGTGCAA 
AGGGAATTAC TGACCGAGTG GAGACAGCAG AGGCCGGTCC TCAAAATCCC TTCAAAACAC 
TCACATAAAT CCTCCAATAG CAGAGACAGG AAGACCTCGA CTAAAGGCAG CCAGTGTAAC 
AACATACTGA AATACTTCAA ACCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

poli ( XM_017356084.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211573.1 Danio rerio polymerase (DNA directed) iota (poli), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59080
Clone ID Related Accession (Same CDS sequence)	XM_017356084.2 , XM_017356084.1
Accession Version	XM_017356084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA polymerase iota isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG GAAGTTCCAC TGATAGAGTT TCATTGACAT CCCAGAGAGT GATCCTGCAT 
TTTGACCTGG ACTGTTTTTA TGCACAAGTT GAGATGATCC GAAATCCAGC TCTCCGGACC 
AAACCTCTTG GGATACAGCA GAAATATATT ATTGTAACCA GTAATTATGT GGCAAGAGAG 
CTTGGCGTGA ACAAGCTGAT GTCAGTGACA GATGCAGTGG AGAAATGTCC ACAGCTGGTG 
CTTGTAAAAG GAGAAGATCT CACTCATTAC AGAGAAATGT CCTACAAAGT CACTGAATTG 
CTGATGTCGT ACTGCCCTCT AGTGGAGCGG CTGGGCTTTG ATGAGAACTT CATGGACGTT 
ACAGAAATGG TGGAGAAACG AATAAAGGAG ACCCGTATTT CAGATCTCTC CTTCAATGGA 
CACGTATACA ATCATGAAAG TGTTGTGGTT GATGAGGAGA ACATTCGTTT GGCTGTCGGC 
TCTGTGATTG CCGCTGAGAT GAGGCAAGCC ATCTTCAGCA CACTGGGGCT AACTGGCTGT 
GCTGGTGTCG CTAGCAACAA ACTATTAGCC AAGCTGGTGT CAGGCAGCTT TAAACCCAAC 
CAGCAGACCA CCCTCCTGCC TCACAGCACC GCTGAACTCA TGAGCAGCCT TACAGGACTC 
ATCAAAGTGC CAGGGATTGG TTACAAGACA CGTGAGAAGC TGAAGGCTCT GGGTTTGGTT 
AATGTGAGAG ATCTACAGTT GTACCCTTTG TCCGATCTGG TGAAAGAGTT TGGAGAGATG 
AACGCTAAGA GGATCCAAAA CCTCGCCTGT GGGATTGATG ATTCTCCAGT GACTCCAGCG 
GGTCCTCCTC AGTCCCTCAG CGATGAGGAC TCATTCAAGA AAATGTCCAC TCTAGAGGAA 
GTTCTAAAGA AAATTGAAGA ACTGCTCATC AACCTTACTG AGAGGATGCA CAAAGATGGC 
AGGCAGCCAC ACACACTGAG ACTAACGATT CGCAAATACA CTGTCACCAA TCGCTGGTTT 
AATCGAGAGA GTCGTCAGTG TCCCATTCCC AACCACACCG GGCTCAAGAT CACAGGAGGA 
AGCAGCGAGG CTGTGCCCCA GTTACTGTCA CTGTCGATGA AGCTCTTTCA TAAAATGGTG 
GACCCACATC AGCCTTTTCA TCTGACTCTG CTCAATGTGT GTTTTAGCAA CCTGCAGGCT 
AAAAGCTCCA GCAAAAGCTC CATCGCTTCT TTTTTTACAC ACAAATCTCC TTCAACAACA 
CAAATCCCAT CTCAGCAGGT GGACTCTGAT CTCTGTGAAT CTGTTGCAAC TTCCCATTTC 
ACTCAAGCAG ATGTTTTGCA TAAAAATGAT AAAGCGTGGA AACCAGTAAA AAACACATCA 
ATATCTCCAA CCCAAACACA GTCGACCTCA GTCAACCCCA AATCTTCCCT ATCTCCCAGA 
CAGCCTGTTT CAGAGCACTC ACAACATGCA AACATAAACA TCAGACCGCC TTGTTTGAGC 
AGCGCTCCCA AAAAAACATT GCATTTCAAG CTGCCTCCTA ATGTTGATGC GGATGTATTC 
AAACTCCTTC CTGAACACAT TCAGATGGAG CTGATGTCCA GTTTTAAAAA GGGAGACTCC 
GTTTACAGAC CCGCTGACAC AGAAGATGTT GACCCAGAAC ATCAACCCAC GTTTGCCTCT 
CGCCAACCAA AAACACCAAA ACCTTGTGAG TCTGCAGTAA ATGTCTGCAC AAATTCTGCT 
TCTAATCCCC ATGAACTCAG CCAGAACTTT TCATCTCCTC AGTCGATCCC GGAGTTCAGT 
TCTGAACTTG TCATGGTCAC AGAAAGTGAC GATATCCCAC ACTCCACTCA GGCCATTCAG 
CCAGAAGTTC CTGCAAATGT GGACCCTTAT GTGTTTTCAC AACTTCCTGC TGATGTGCAA 
AGGGAATTAC TGACCGAGTG GAGACAGCAG AGGCCGGTCC TCAAAATCCC TTCAAAACAC 
TCACATAAAT CCTCCAATAG CAGAGACAGG AAGACCTCGA CTAAAGGCAG CCAGTGTAAC 
AACATACTGA AATACTTCAA ACCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211573.1
CDS	117..2246
Translation	MDSDEEEDNNDWERSLESDILETGSSTDRVSLTSQRVILHFDLDCFYAQVEMIRNPALRTKPLGIQQKYIIVTSNYVARELGVNKLMSVTDAVEKCPQLVLVKGEDLTHYREMSYKVTELLMSYCPLVERLGFDENFMDVTEMVEKRIKETRISDLSFNGHVYNHESVVVDEENIRLAVGSVIAAEMRQAIFSTLGLTGCAGVASNKLLAKLVSGSFKPNQQTTLLPHSTAELMSSLTGLIKVPGIGYKTREKLKALGLVNVRDLQLYPLSDLVKEFGEMNAKRIQNLACGIDDSPVTPAGPPQSLSDEDSFKKMSTLEEVLKKIEELLINLTERMHKDGRQPHTLRLTIRKYTVTNRWFNRESRQCPIPNHTGLKITGGSSEAVPQLLSLSMKLFHKMVDPHQPFHLTLLNVCFSNLQAKSSSKSSIASFFTHKSPSTTQIPSQQVDSDLCESVATSHFTQADVLHKNDKAWKPVKNTSISPTQTQSTSVNPKSSLSPRQPVSEHSQHANINIRPPCLSSAPKKTLHFKLPPNVDADVFKLLPEHIQMELMSSFKKGDSVYRPADTEDVDPEHQPTFASRQPKTPKPCESAVNVCTNSASNPHELSQNFSSPQSIPEFSSELVMVTESDDIPHSTQAIQPEVPANVDPYVFSQLPADVQRELLTEWRQQRPVLKIPSKHSHKSSNSRDRKTSTKGSQCNNILKYFKPN

Target ORF information:

RefSeq Version	XM_017356084.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio polymerase (DNA directed) iota (poli), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356084.1

ORF Insert Sequence:

ATGGACTCGG ACGAAGAGGA AGACAATAAT GACTGGGAAC GCAGTTTAGA GTCAGACATT 
TTGGAGACTG GAAGTTCCAC TGATAGAGTT TCATTGACAT CCCAGAGAGT GATCCTGCAT 
TTTGACCTGG ACTGTTTTTA TGCACAAGTT GAGATGATCC GAAATCCAGC TCTCCGGACC 
AAACCTCTTG GGATACAGCA GAAATATATT ATTGTAACCA GTAATTATGT GGCAAGAGAG 
CTTGGCGTGA ACAAGCTGAT GTCAGTGACA GATGCAGTGG AGAAATGTCC ACAGCTGGTG 
CTTGTAAAAG GAGAAGATCT CACTCATTAC AGAGAAATGT CCTACAAAGT CACTGAATTG 
CTGATGTCGT ACTGCCCTCT AGTGGAGCGG CTGGGCTTTG ATGAGAACTT CATGGACGTT 
ACAGAAATGG TGGAGAAACG AATAAAGGAG ACCCGTATTT CAGATCTCTC CTTCAATGGA 
CACGTATACA ATCATGAAAG TGTTGTGGTT GATGAGGAGA ACATTCGTTT GGCTGTCGGC 
TCTGTGATTG CCGCTGAGAT GAGGCAAGCC ATCTTCAGCA CACTGGGGCT AACTGGCTGT 
GCTGGTGTCG CTAGCAACAA ACTATTAGCC AAGCTGGTGT CAGGCAGCTT TAAACCCAAC 
CAGCAGACCA CCCTCCTGCC TCACAGCACC GCTGAACTCA TGAGCAGCCT TACAGGACTC 
ATCAAAGTGC CAGGGATTGG TTACAAGACA CGTGAGAAGC TGAAGGCTCT GGGTTTGGTT 
AATGTGAGAG ATCTACAGTT GTACCCTTTG TCCGATCTGG TGAAAGAGTT TGGAGAGATG 
AACGCTAAGA GGATCCAAAA CCTCGCCTGT GGGATTGATG ATTCTCCAGT GACTCCAGCG 
GGTCCTCCTC AGTCCCTCAG CGATGAGGAC TCATTCAAGA AAATGTCCAC TCTAGAGGAA 
GTTCTAAAGA AAATTGAAGA ACTGCTCATC AACCTTACTG AGAGGATGCA CAAAGATGGC 
AGGCAGCCAC ACACACTGAG ACTAACGATT CGCAAATACA CTGTCACCAA TCGCTGGTTT 
AATCGAGAGA GTCGTCAGTG TCCCATTCCC AACCACACCG GGCTCAAGAT CACAGGAGGA 
AGCAGCGAGG CTGTGCCCCA GTTACTGTCA CTGTCGATGA AGCTCTTTCA TAAAATGGTG 
GACCCACATC AGCCTTTTCA TCTGACTCTG CTCAATGTGT GTTTTAGCAA CCTGCAGGCT 
AAAAGCTCCA GCAAAAGCTC CATCGCTTCT TTTTTTACAC ACAAATCTCC TTCAACAACA 
CAAATCCCAT CTCAGCAGGT GGACTCTGAT CTCTGTGAAT CTGTTGCAAC TTCCCATTTC 
ACTCAAGCAG ATGTTTTGCA TAAAAATGAT AAAGCGTGGA AACCAGTAAA AAACACATCA 
ATATCTCCAA CCCAAACACA GTCGACCTCA GTCAACCCCA AATCTTCCCT ATCTCCCAGA 
CAGCCTGTTT CAGAGCACTC ACAACATGCA AACATAAACA TCAGACCGCC TTGTTTGAGC 
AGCGCTCCCA AAAAAACATT GCATTTCAAG CTGCCTCCTA ATGTTGATGC GGATGTATTC 
AAACTCCTTC CTGAACACAT TCAGATGGAG CTGATGTCCA GTTTTAAAAA GGGAGACTCC 
GTTTACAGAC CCGCTGACAC AGAAGATGTT GACCCAGAAC ATCAACCCAC GTTTGCCTCT 
CGCCAACCAA AAACACCAAA ACCTTGTGAG TCTGCAGTAA ATGTCTGCAC AAATTCTGCT 
TCTAATCCCC ATGAACTCAG CCAGAACTTT TCATCTCCTC AGTCGATCCC GGAGTTCAGT 
TCTGAACTTG TCATGGTCAC AGAAAGTGAC GATATCCCAC ACTCCACTCA GGCCATTCAG 
CCAGAAGTTC CTGCAAATGT GGACCCTTAT GTGTTTTCAC AACTTCCTGC TGATGTGCAA 
AGGGAATTAC TGACCGAGTG GAGACAGCAG AGGCCGGTCC TCAAAATCCC TTCAAAACAC 
TCACATAAAT CCTCCAATAG CAGAGACAGG AAGACCTCGA CTAAAGGCAG CCAGTGTAAC 
AACATACTGA AATACTTCAA ACCCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Transcriptome analysis reveals positive selection on the divergent between topmouth culter and zebrafish.
Gene552(2)265-71(2014 Dec)
Ren L,Tan XJ,Xiong YF,Xu K,Zhou Y,Zhong H,Liu Y,Hong YH,Liu SJ

Expressed sequence tag analysis of expression profiles of zebrafish testis and ovary.
Gene294(1-2)45-53(2002 Jul)
Zeng S,Gong Z

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poli cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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