ca8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ca8
Entrez Gene ID	550233
Full Name	carbonic anhydrase VIII
Synonyms	zgc:110118
General protein information	Preferred Names carbonic anhydrase VIII Names carbonic anhydrase-related protein CARP VIII
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017571.1	NP_001017571.1	carbonic anhydrase-related protein
NM_001017571.2	NP_001017571.2	carbonic anhydrase-related protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	CA8	NP_004047.3
Rattus norvegicus (Norway rat)	Car8	NP_001009662.1
Bos taurus (cattle)	CA8	NP_001077159.1
Mus musculus (house mouse)	Car8	NP_031618.2
Xenopus tropicalis (tropical clawed frog)	ca8	NP_001011213.1
Pan troglodytes (chimpanzee)	CA8	XP_519778.2
	CA8	XP_001088977.1
Gallus gallus (chicken)	CA8	XP_004939926.1
Danio rerio (zebrafish)	ca8	NP_001017571.1
Canis lupus familiaris (dog)	CA8	XP_544094.2

Gene Ontology
Bibliography

Related articles in PubMed

Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear.
Matsumoto H, Fujiwara S, Miyagi H, Nakamura N, Shiga Y, Ohta T, Tsuzuki M
Marine biotechnology (New York, N.Y.)19(5)430-440(2017 Oct)

Gene expression profiling of granule cells and Purkinje cells in the zebrafish cerebellum.
Takeuchi M, Yamaguchi S, Sakakibara Y, Hayashi T, Matsuda K, Hara Y, Tanegashima C, Shimizu T, Kuraku S, Hibi M
The Journal of comparative neurology525(7)1558-1585(2017 May)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells.
Tarbashevich K, Reichman-Fried M, Grimaldi C, Raz E
Current biology : CB25(8)1096-103(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CA8 overexpression desensitizes neuronal cells to STS induced apoptotic stress and increases cell migration and invasion ability in neuronal cells.
Title: Roles of carbonic anhydrase 8 in neuronal cells and zebrafish.

We provide evidence that the CA8 gene product is indispensable in early embryonic development in zebra fi sh, particularly in the brain and eye.
Title: Abnormal cerebellar development and ataxia in CARP VIII morphant zebrafish.

The following ca8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ca8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32489	NM_001017571.1 Latest version!	Danio rerio carbonic anhydrase VIII (ca8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa159795	NM_001017571.2 Latest version!	Danio rerio carbonic anhydrase VIII (ca8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ca8 ( NM_001017571.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017571.1 Danio rerio carbonic anhydrase VIII (ca8), mRNA.

Price & Availability↑

CloneID	ODa32489
Clone ID Related Accession (Same CDS sequence)	NM_001017571.1
Accession Version	NM_001017571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-13
Organism	Danio rerio(zebrafish)
Product	carbonic anhydrase-related protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC092740.1. ##Evidence-Data-START## Transcript exon combination :: BC092740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGATA ATGTTATTGA AGAGAGCGGT AATTACCCTG GAAAGGATGA GCTGGACTGG 
GGATATGAAG AAGGTGTTGA ATGGGGTCTT CTGTTTCCTG AGGCCAATGG AGAGTACCAG 
TCCCCTATAA ACCTCAACTC ACGCGAGGCT CGCTATGACC CGCAGCTGCT GGACGTGGGG 
CTCAACCCAA ACTATGTGGT CTGCCGCGAC TGTGAAGTCA TTAATGATGG TCACACTGTG 
AGGATCATGC TGAAATCCAA ATCTGTTGTT ACGGGAGGTC CTCTCCCCAG TGACCATGAG 
TATGAGCTGA GCGAGGTGCG CTTTCACTGG GGCAAAGAAA ACCAGAGAGG ATCTGAACAC 
ACTGTCAACT TCAAGGCTTT TCCAATGGAG CTTCATCTAA TACACTGGAA CTCTACATTA 
TTCAACAGTG TGGAGGAGGC CATGGGCAAG AAAAGAGGCA TTCTCATCAT CGCCCTGTTT 
GTGCAGGTTG GGAAGGAACA TCTTGGTCTG AAGGCCATCA CAGACGTCCT GCAGGACCTG 
CAGTATAAGG GGAAGACTAA AATAATTCCA TGTTTTAATC CCAACACATT ACTCCCTGAC 
CCTCTATTAA GAGATTACTG GGTGTATGAG GGCTCTTTAA CCACCCCGCC CTGTAGTGAG 
AACGTCACCT GGATTCTGTA CCGTTACCCT TTGACCATCT CTCAGATGCA GATTGAAGAG 
TTTCGTCGCC TTAGGCCCCA TGTTAAAGGA GCAGAGCTTC CAGAAGGCAA TGATGGGATG 
CTGGGGGACA ACTTTAGACC GACCCAACCA CTGAGCGACA GGGTGGTCAG GGCAGCCTTC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017571.1
CDS	296..1141
Translation	MADNVIEESGNYPGKDELDWGYEEGVEWGLLFPEANGEYQSPINLNSREARYDPQLLDVGLNPNYVVCRDCEVINDGHTVRIMLKSKSVVTGGPLPSDHEYELSEVRFHWGKENQRGSEHTVNFKAFPMELHLIHWNSTLFNSVEEAMGKKRGILIIALFVQVGKEHLGLKAITDVLQDLQYKGKTKIIPCFNPNTLLPDPLLRDYWVYEGSLTTPPCSENVTWILYRYPLTISQMQIEEFRRLRPHVKGAELPEGNDGMLGDNFRPTQPLSDRVVRAAFQ

Target ORF information:

RefSeq Version	NM_001017571.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbonic anhydrase VIII (ca8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017571.1

ORF Insert Sequence:

ATGGCTGATA ATGTTATTGA AGAGAGCGGT AATTACCCTG GAAAGGATGA GCTGGACTGG 
GGATATGAAG AAGGTGTTGA ATGGGGTCTT CTGTTTCCTG AGGCCAATGG AGAGTACCAG 
TCCCCTATAA ACCTCAACTC ACGCGAGGCT CGCTATGACC CGCAGCTGCT GGACGTGGGG 
CTCAACCCAA ACTATGTGGT CTGCCGCGAC TGTGAAGTCA TTAATGATGG TCACACTGTG 
AGGATCATGC TGAAATCCAA ATCTGTTGTT ACGGGAGGTC CTCTCCCCAG TGACCATGAG 
TATGAGCTGA GCGAGGTGCG CTTTCACTGG GGCAAAGAAA ACCAGAGAGG ATCTGAACAC 
ACTGTCAACT TCAAGGCTTT TCCAATGGAG CTTCATCTAA TACACTGGAA CTCTACATTA 
TTCAACAGTG TGGAGGAGGC CATGGGCAAG AAAAGAGGCA TTCTCATCAT CGCCCTGTTT 
GTGCAGGTTG GGAAGGAACA TCTTGGTCTG AAGGCCATCA CAGACGTCCT GCAGGACCTG 
CAGTATAAGG GGAAGACTAA AATAATTCCA TGTTTTAATC CCAACACATT ACTCCCTGAC 
CCTCTATTAA GAGATTACTG GGTGTATGAG GGCTCTTTAA CCACCCCGCC CTGTAGTGAG 
AACGTCACCT GGATTCTGTA CCGTTACCCT TTGACCATCT CTCAGATGCA GATTGAAGAG 
TTTCGTCGCC TTAGGCCCCA TGTTAAAGGA GCAGAGCTTC CAGAAGGCAA TGATGGGATG 
CTGGGGGACA ACTTTAGACC GACCCAACCA CTGAGCGACA GGGTGGTCAG GGCAGCCTTC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ca8 ( NM_001017571.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001017571.2 Danio rerio carbonic anhydrase VIII (ca8), mRNA.

Price & Availability↑

CloneID	ODa159795
Clone ID Related Accession (Same CDS sequence)	NM_001017571.2
Accession Version	NM_001017571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	carbonic anhydrase-related protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR847864.6. On Apr 20, 2018 this sequence version replaced NM_001017571.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC092740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGATA ATGTTATTGA AGAGAGCGGT AATTACCCTG GAAAGGATGA GCTGGACTGG 
GGATATGAAG AAGGTGTTGA ATGGGGTCTT CTGTTTCCTG AGGCCAATGG AGAGTACCAG 
TCCCCTATAA ACCTCAACTC ACGCGAGGCT CGCTATGACC CGCAGCTGCT GGACGTGGGG 
CTCAACCCAA ACTATGTGGT CTGCCGCGAC TGTGAAGTCA TTAATGATGG TCACACTGTG 
AGGATCATGC TGAAATCCAA ATCTGTTGTT ACTGGAGGTC CTCTCCCCAG TGACCATGAG 
TATGAGCTGA GCGAGGTGCG CTTTCACTGG GGCAAAGAAA ACCAGAGAGG ATCTGAACAC 
ACTGTCAACT TCAAGGCTTT TCCAATGGAG CTTCATCTAA TACACTGGAA CTCTACATTA 
TTCAACAGCG TGGAGGAGGC CATGGGCAAG AAAAGAGGCA TTCTCATCAT CGCCCTGTTT 
GTGCAGGTTG GGAAGGAACA TCTTGGTCTG AAGGCCATCA CAGACGTCCT GCAGGACCTG 
CAGTATAAGG GGAAGACTAA AATAATTCCA TGTTTTAATC CCAACACATT ACTCCCTGAT 
CCTCTATTAA GAGATTACTG GGTGTATGAG GGCTCTTTAA CCACCCCGCC CTGTAGTGAG 
AACGTCACCT GGATTCTGTA CCGTTACCCT TTGACCATCT CTCAGATGCA GATTGAAGAG 
TTTCGTCGCC TTAGGTCCCA TGTTAAAGGA GCAGAGCTTC CAGAAGGCAA TGATGGGATG 
CTGGGGGACA ACTTTAGACC GACCCAACCA CTGAGTGACA GGGTGGTCAG GGCAGCCTTC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017571.2
CDS	414..1259
Translation	MADNVIEESGNYPGKDELDWGYEEGVEWGLLFPEANGEYQSPINLNSREARYDPQLLDVGLNPNYVVCRDCEVINDGHTVRIMLKSKSVVTGGPLPSDHEYELSEVRFHWGKENQRGSEHTVNFKAFPMELHLIHWNSTLFNSVEEAMGKKRGILIIALFVQVGKEHLGLKAITDVLQDLQYKGKTKIIPCFNPNTLLPDPLLRDYWVYEGSLTTPPCSENVTWILYRYPLTISQMQIEEFRRLRSHVKGAELPEGNDGMLGDNFRPTQPLSDRVVRAAFQ

Target ORF information:

RefSeq Version	NM_001017571.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carbonic anhydrase VIII (ca8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017571.2

ORF Insert Sequence:

ATGGCTGATA ATGTTATTGA AGAGAGCGGT AATTACCCTG GAAAGGATGA GCTGGACTGG 
GGATATGAAG AAGGTGTTGA ATGGGGTCTT CTGTTTCCTG AGGCCAATGG AGAGTACCAG 
TCCCCTATAA ACCTCAACTC ACGCGAGGCT CGCTATGACC CGCAGCTGCT GGACGTGGGG 
CTCAACCCAA ACTATGTGGT CTGCCGCGAC TGTGAAGTCA TTAATGATGG TCACACTGTG 
AGGATCATGC TGAAATCCAA ATCTGTTGTT ACTGGAGGTC CTCTCCCCAG TGACCATGAG 
TATGAGCTGA GCGAGGTGCG CTTTCACTGG GGCAAAGAAA ACCAGAGAGG ATCTGAACAC 
ACTGTCAACT TCAAGGCTTT TCCAATGGAG CTTCATCTAA TACACTGGAA CTCTACATTA 
TTCAACAGCG TGGAGGAGGC CATGGGCAAG AAAAGAGGCA TTCTCATCAT CGCCCTGTTT 
GTGCAGGTTG GGAAGGAACA TCTTGGTCTG AAGGCCATCA CAGACGTCCT GCAGGACCTG 
CAGTATAAGG GGAAGACTAA AATAATTCCA TGTTTTAATC CCAACACATT ACTCCCTGAT 
CCTCTATTAA GAGATTACTG GGTGTATGAG GGCTCTTTAA CCACCCCGCC CTGTAGTGAG 
AACGTCACCT GGATTCTGTA CCGTTACCCT TTGACCATCT CTCAGATGCA GATTGAAGAG 
TTTCGTCGCC TTAGGTCCCA TGTTAAAGGA GCAGAGCTTC CAGAAGGCAA TGATGGGATG 
CTGGGGGACA ACTTTAGACC GACCCAACCA CTGAGTGACA GGGTGGTCAG GGCAGCCTTC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Carbonic Anhydrase Inhibitors Induce Developmental Toxicity During Zebrafish Embryogenesis, Especially in the Inner Ear.
Marine biotechnology (New York, N.Y.)19(5)430-440(2017 Oct)
Matsumoto H,Fujiwara S,Miyagi H,Nakamura N,Shiga Y,Ohta T,Tsuzuki M

Gene expression profiling of granule cells and Purkinje cells in the zebrafish cerebellum.
The Journal of comparative neurology525(7)1558-1585(2017 May)
Takeuchi M,Yamaguchi S,Sakakibara Y,Hayashi T,Matsuda K,Hara Y,Tanegashima C,Shimizu T,Kuraku S,Hibi M

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Chemokine-Dependent pH Elevation at the Cell Front Sustains Polarity in Directionally Migrating Zebrafish Germ Cells.
Current biology : CB25(8)1096-103(2015 Apr)
Tarbashevich K,Reichman-Fried M,Grimaldi C,Raz E