enpep cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	enpep
Entrez Gene ID	504088
Full Name	glutamyl aminopeptidase
Synonyms	im:7152184,si:ch211-146m5.2
General protein information	Preferred Names glutamyl aminopeptidase Names glutamyl aminopeptidase aminopeptidase A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001199731.1	NP_001186660.1	glutamyl aminopeptidase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ENPEP	NP_001968.3
	ENPEP	XP_001090456.1
Mus musculus (house mouse)	Enpep	NP_031960.1
Drosophila melanogaster (fruit fly)	CG32473	NP_731786.1
Gallus gallus (chicken)	ENPEP	XP_426327.3
Xenopus tropicalis (tropical clawed frog)	enpep	XP_002934295.1
Pan troglodytes (chimpanzee)	ENPEP	XP_517397.2
Canis lupus familiaris (dog)	ENPEP	XP_535696.2
Drosophila melanogaster (fruit fly)	CG8773	NP_650273.2
Drosophila melanogaster (fruit fly)	CG8774	NP_650274.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003077	XP_003436384.1
Bos taurus (cattle)	ENPEP	NP_001033116.1
Rattus norvegicus (Norway rat)	Enpep	NP_071587.2
Danio rerio (zebrafish)	enpep	NP_001186660.1

Gene Ontology
Bibliography

Related articles in PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Pan Y, Jiang S, Hou Q, Qiu D, Shi J, Wang L, Chen Z, Zhang M, Duan A, Qin W, Zen K, Liu Z
Diabetes(2017 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Multiplexed 3D FRET imaging in deep tissue of live embryos.
Zhao M, Wan X, Li Y, Zhou W, Peng L
Scientific reports513991(2015 Sep)

The Lowe syndrome protein OCRL1 is required for endocytosis in the zebrafish pronephric tubule.
Oltrabella F, Pietka G, Ramirez IB, Mironov A, Starborg T, Drummond IA, Hinchliffe KA, Lowe M
PLoS genetics11(4)e1005058(2015 Apr)

Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene.
Seiler C, Pack M
Gene expression patterns : GEP11(1-2)118-21(2011 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study describes a zebrafish 2 kb glutamyl aminopeptidase (enpep) promoter fragment, and shows that it can drive gene expression specifically in the kidney during early and late development.
Title: Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene.

The following enpep gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the enpep cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31523	NM_001199731.1 Latest version!	Danio rerio glutamyl aminopeptidase (enpep), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

enpep ( NM_001199731.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001186660.1 Danio rerio glutamyl aminopeptidase (enpep), mRNA.

Price & Availability↑

CloneID	ODa31523
Clone ID Related Accession (Same CDS sequence)	NM_001199731.1
Accession Version	NM_001199731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	glutamyl aminopeptidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR559943.6, CU041321.5 and CT025771.7. On Dec 15, 2010 this sequence version replaced XR_029637.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01009660.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAAAGCC TGGACTTCGA GAAGAAGGAA AAGCGGTACT GTATCCGAGG AAAGCACGCT 
GCTATCATCT GTGCCGTCAT CGTAGTGACA GGTGTAGCTG TGGGACTTGG AGTTGGCTTA 
AGCTTGAATT CTTCATCCGA AAAGGAAACA CCATTACCAA CTCCATTACC CACAGAAGTG 
CCTGATGATC GCCCATGCAA ACCATCTGAG AACACCAATG GCGGCTGGAG CAATTTCCGA 
TTACCTGACT ACATTCTTCC GTTCCATTAC GATCTGCATA TGGAGCCTGA CCTCAACACG 
GACATCTACA CTGGGGACGT GTCTGTACAT CTGAAACTGA CTCAGCCCAG TCAGCACCTG 
TGGCTCCACA TCCGGGACAC TTTTGTCACT GTGATGCCTT CACTACAGCG CAGCAGCCCG 
TCTGGTGTGA CCTCTGTCAA GTTGAAGCAA TGCTTTGAAT ACAAGCCGCA GGAGTACGTG 
GTTGTGGAAG CGGCTGAGCA GTTGAGCGTT ACAGGGCCGG ATGAACACTA CGTCCTCACT 
CTGCACTTCC AGGGCTGGTT AAATGGCTCT CTGGTTGGCT TCTACAGGAC CACGTATCAG 
GAGAATGGAG TTACCAAGAA AATCGCTGCA ACAGACCATG AACCCACGGA CGCTCGTAAA 
TCTTTTCCAT GTTTTGATGA GCCCAACAAA AAGGCCACCT ACACAATCTC CATCACCCAT 
GACAGTACAT ATAAAGCACT GTCCAACATG CCTGTGGAGA AAACAGAAAA ACTTTCAGAA 
CAAAAGACTA AAACATCCTT CATGAAGTCA GTAAAGATGA GCACTTATCT GGTGTGTTTT 
GCGGTGCATC AGTTCGATTT TGTGGAGCGG ACCTCGAAAA GAGGAATACC GCTGCGGATC 
TATGCTCAGC CTTTGCAGAT CTCCACGGCT GCATATGCTG CAGATGTCAC ACAGGTCATA 
TTCGATTACT TTGAGGAATA TTTCGACATG GAATATTCAA TACAAAAACT TGATAAGATA 
GCGATCCCAG ACTTTGGGAC AGGAGCGATG GAGAACTGGG GCTTGATCAC TTACAGAGAA 
ACCAACCTGC TGTTCGATGA GAAGGAGTCA TCCTCGGTCA ACAAGCAGCG AGTGGCCAGT 
GTCATTGCAC ACGAGCTTGT CCATCAGTGG TTTGGAAATA TTGTGACTAT GGACTGGTGG 
GATGACCTGT GGCTGAACGA GGGCTTTGCC AGCTTTTTTG AGTATATTGG AGTGGAAGAA 
GCTGAGCATG ACTGGGGAAT GCGTGACGTC ATGCTTATCA ACGATGTATA TCCTGTCATG 
GTCGATGATG CCCTTCTCTC CTCGCATCCC ATTATTGTGG ATGTGTCGAG CCCAGCTGAG 
ATCACATCTG TGTTTGATGC CATCTCATAT AACAAGGGAG CTTCTATTTT GCGAATGCTG 
GAGGACTTAC TTGGCCGAGA GACATTTAGG GATGGTTGTC GAAGATATCT GAAGACCTAC 
CTCTTCCAGA ACGCAAAGAC TTCAGACTTC TGGAAAGCCT TGGCTGATGA GAGTGGCTTA 
CCCGTGGCAG ACATTATGGA CACATGGACC AAGCAGATGG GTTATCCAGT GCTCAGTCTG 
ACCAACACAG ACACTGAAGC TAAACTCACG CAGACCAGGT TTCTTCTGGA CCCTAATGCT 
GACCCTAGTC AGCCCACCAC TCCACTGGGG TATAAATGGA CTATTCCAGT GAAATGGAAA 
GCACTTGACT CCACAAACAA CTCTTTTATA TTTGAAAAAG GACAAACAGA GGCAGTCATT 
AGTGGATACT CGCATGCTAC CAATGGTCTT ATCAAAGTCA ACAAAGACCA CATGGGCTTC 
TACAGAGTAA ATCATCATGA CCAAATGTGG AGCGATATTG CTGAACAACT TCTTATGGAT 
CATCAGGTCT ACGATGCAAC TGACAGAAGC AGCTACATTG ATGATATATT CGCATTAGGA 
CGGGCAGATA TGGTTGATTA CGGGAATGCC TTCAATTTAA CAAGATATCT GGCTGACGAA 
ACCGAATATA TTGTGTGGGA TCGAGTTTCT GCTTCTATCT CCTACGTGAG AGAAATGTTG 
GCTGATGATA CAGTGCTGTA CCCCTTATTT CAGAAATTAT TCAGGGGTCA CGTGCAGAAG 
ATATCCAGAG AGCTTGGCTG GAAGGATGAG GGGAATCAAA CACAGCGGCT GCTGAGAGAG 
ATCGTTTTGG GCATTGCATG TCAAATGGGT GACCAGGAAG CTCTTGACCA AGCCTCTGAT 
ATTTTCAATA AATGGATTAA AGGAACAATC GGCAGTGTGC CTGTGAACTT GCGTCTGCTG 
GTATATCGGT ACGGCATGAT GAACTCAGGA ACAGAGGAGA GCTGGGAAAT CATGTTCCAG 
AAGTATCTGT CTGCCACTCT GGCCCAGGAG AAAGATAAGC TGCTCTATGG TCTGGCTTCA 
GTCAAAAATA TTCATCTGCT TCATAGGTTG CTTGAAGCCA CAAAAAATGA AAGCATCATA 
AGGAGTCAAG ATGTTTTTAC TTTAGTGCAA TACGTGTCAC GAAGCTCTGA TGGTAAAATT 
ATGGCCTGGG ACTGGATGAC GCTCAACTGG GATTACCTGG TGAACAGATA CACTATCAAT 
GACAGAAATT TGGGCCGCCT GCCAGCCAGA ATTACAACTA CATACAGCTC TAATCTTCAA 
CTGTGGAAGA TGGAGCACTT TTTTGCCCTG CACCCGAATG CAGGAGCAGG TGAGATGCCC 
AGAAAGCAGG CACTGGAGAC TGTGAAGAAC AATATCGAAT GGGTGGAAAG GAATAAGGAT 
GAGATCCGGT TCTGGCTGGA GAACAATGTG TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186660.1
CDS	1..2856
Translation	MESLDFEKKEKRYCIRGKHAAIICAVIVVTGVAVGLGVGLSLNSSSEKETPLPTPLPTEVPDDRPCKPSENTNGGWSNFRLPDYILPFHYDLHMEPDLNTDIYTGDVSVHLKLTQPSQHLWLHIRDTFVTVMPSLQRSSPSGVTSVKLKQCFEYKPQEYVVVEAAEQLSVTGPDEHYVLTLHFQGWLNGSLVGFYRTTYQENGVTKKIAATDHEPTDARKSFPCFDEPNKKATYTISITHDSTYKALSNMPVEKTEKLSEQKTKTSFMKSVKMSTYLVCFAVHQFDFVERTSKRGIPLRIYAQPLQISTAAYAADVTQVIFDYFEEYFDMEYSIQKLDKIAIPDFGTGAMENWGLITYRETNLLFDEKESSSVNKQRVASVIAHELVHQWFGNIVTMDWWDDLWLNEGFASFFEYIGVEEAEHDWGMRDVMLINDVYPVMVDDALLSSHPIIVDVSSPAEITSVFDAISYNKGASILRMLEDLLGRETFRDGCRRYLKTYLFQNAKTSDFWKALADESGLPVADIMDTWTKQMGYPVLSLTNTDTEAKLTQTRFLLDPNADPSQPTTPLGYKWTIPVKWKALDSTNNSFIFEKGQTEAVISGYSHATNGLIKVNKDHMGFYRVNHHDQMWSDIAEQLLMDHQVYDATDRSSYIDDIFALGRADMVDYGNAFNLTRYLADETEYIVWDRVSASISYVREMLADDTVLYPLFQKLFRGHVQKISRELGWKDEGNQTQRLLREIVLGIACQMGDQEALDQASDIFNKWIKGTIGSVPVNLRLLVYRYGMMNSGTEESWEIMFQKYLSATLAQEKDKLLYGLASVKNIHLLHRLLEATKNESIIRSQDVFTLVQYVSRSSDGKIMAWDWMTLNWDYLVNRYTINDRNLGRLPARITTTYSSNLQLWKMEHFFALHPNAGAGEMPRKQALETVKNNIEWVERNKDEIRFWLENNVS

Target ORF information:

RefSeq Version	NM_001199731.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio glutamyl aminopeptidase (enpep), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199731.1

ORF Insert Sequence:

ATGGAAAGCC TGGACTTCGA GAAGAAGGAA AAGCGGTACT GTATCCGAGG AAAGCACGCT 
GCTATCATCT GTGCCGTCAT CGTAGTGACA GGTGTAGCTG TGGGACTTGG AGTTGGCTTA 
AGCTTGAATT CTTCATCCGA AAAGGAAACA CCATTACCAA CTCCATTACC CACAGAAGTG 
CCTGATGATC GCCCATGCAA ACCATCTGAG AACACCAATG GCGGCTGGAG CAATTTCCGA 
TTACCTGACT ACATTCTTCC GTTCCATTAC GATCTGCATA TGGAGCCTGA CCTCAACACG 
GACATCTACA CTGGGGACGT GTCTGTACAT CTGAAACTGA CTCAGCCCAG TCAGCACCTG 
TGGCTCCACA TCCGGGACAC TTTTGTCACT GTGATGCCTT CACTACAGCG CAGCAGCCCG 
TCTGGTGTGA CCTCTGTCAA GTTGAAGCAA TGCTTTGAAT ACAAGCCGCA GGAGTACGTG 
GTTGTGGAAG CGGCTGAGCA GTTGAGCGTT ACAGGGCCGG ATGAACACTA CGTCCTCACT 
CTGCACTTCC AGGGCTGGTT AAATGGCTCT CTGGTTGGCT TCTACAGGAC CACGTATCAG 
GAGAATGGAG TTACCAAGAA AATCGCTGCA ACAGACCATG AACCCACGGA CGCTCGTAAA 
TCTTTTCCAT GTTTTGATGA GCCCAACAAA AAGGCCACCT ACACAATCTC CATCACCCAT 
GACAGTACAT ATAAAGCACT GTCCAACATG CCTGTGGAGA AAACAGAAAA ACTTTCAGAA 
CAAAAGACTA AAACATCCTT CATGAAGTCA GTAAAGATGA GCACTTATCT GGTGTGTTTT 
GCGGTGCATC AGTTCGATTT TGTGGAGCGG ACCTCGAAAA GAGGAATACC GCTGCGGATC 
TATGCTCAGC CTTTGCAGAT CTCCACGGCT GCATATGCTG CAGATGTCAC ACAGGTCATA 
TTCGATTACT TTGAGGAATA TTTCGACATG GAATATTCAA TACAAAAACT TGATAAGATA 
GCGATCCCAG ACTTTGGGAC AGGAGCGATG GAGAACTGGG GCTTGATCAC TTACAGAGAA 
ACCAACCTGC TGTTCGATGA GAAGGAGTCA TCCTCGGTCA ACAAGCAGCG AGTGGCCAGT 
GTCATTGCAC ACGAGCTTGT CCATCAGTGG TTTGGAAATA TTGTGACTAT GGACTGGTGG 
GATGACCTGT GGCTGAACGA GGGCTTTGCC AGCTTTTTTG AGTATATTGG AGTGGAAGAA 
GCTGAGCATG ACTGGGGAAT GCGTGACGTC ATGCTTATCA ACGATGTATA TCCTGTCATG 
GTCGATGATG CCCTTCTCTC CTCGCATCCC ATTATTGTGG ATGTGTCGAG CCCAGCTGAG 
ATCACATCTG TGTTTGATGC CATCTCATAT AACAAGGGAG CTTCTATTTT GCGAATGCTG 
GAGGACTTAC TTGGCCGAGA GACATTTAGG GATGGTTGTC GAAGATATCT GAAGACCTAC 
CTCTTCCAGA ACGCAAAGAC TTCAGACTTC TGGAAAGCCT TGGCTGATGA GAGTGGCTTA 
CCCGTGGCAG ACATTATGGA CACATGGACC AAGCAGATGG GTTATCCAGT GCTCAGTCTG 
ACCAACACAG ACACTGAAGC TAAACTCACG CAGACCAGGT TTCTTCTGGA CCCTAATGCT 
GACCCTAGTC AGCCCACCAC TCCACTGGGG TATAAATGGA CTATTCCAGT GAAATGGAAA 
GCACTTGACT CCACAAACAA CTCTTTTATA TTTGAAAAAG GACAAACAGA GGCAGTCATT 
AGTGGATACT CGCATGCTAC CAATGGTCTT ATCAAAGTCA ACAAAGACCA CATGGGCTTC 
TACAGAGTAA ATCATCATGA CCAAATGTGG AGCGATATTG CTGAACAACT TCTTATGGAT 
CATCAGGTCT ACGATGCAAC TGACAGAAGC AGCTACATTG ATGATATATT CGCATTAGGA 
CGGGCAGATA TGGTTGATTA CGGGAATGCC TTCAATTTAA CAAGATATCT GGCTGACGAA 
ACCGAATATA TTGTGTGGGA TCGAGTTTCT GCTTCTATCT CCTACGTGAG AGAAATGTTG 
GCTGATGATA CAGTGCTGTA CCCCTTATTT CAGAAATTAT TCAGGGGTCA CGTGCAGAAG 
ATATCCAGAG AGCTTGGCTG GAAGGATGAG GGGAATCAAA CACAGCGGCT GCTGAGAGAG 
ATCGTTTTGG GCATTGCATG TCAAATGGGT GACCAGGAAG CTCTTGACCA AGCCTCTGAT 
ATTTTCAATA AATGGATTAA AGGAACAATC GGCAGTGTGC CTGTGAACTT GCGTCTGCTG 
GTATATCGGT ACGGCATGAT GAACTCAGGA ACAGAGGAGA GCTGGGAAAT CATGTTCCAG 
AAGTATCTGT CTGCCACTCT GGCCCAGGAG AAAGATAAGC TGCTCTATGG TCTGGCTTCA 
GTCAAAAATA TTCATCTGCT TCATAGGTTG CTTGAAGCCA CAAAAAATGA AAGCATCATA 
AGGAGTCAAG ATGTTTTTAC TTTAGTGCAA TACGTGTCAC GAAGCTCTGA TGGTAAAATT 
ATGGCCTGGG ACTGGATGAC GCTCAACTGG GATTACCTGG TGAACAGATA CACTATCAAT 
GACAGAAATT TGGGCCGCCT GCCAGCCAGA ATTACAACTA CATACAGCTC TAATCTTCAA 
CTGTGGAAGA TGGAGCACTT TTTTGCCCTG CACCCGAATG CAGGAGCAGG TGAGATGCCC 
AGAAAGCAGG CACTGGAGAC TGTGAAGAAC AATATCGAAT GGGTGGAAAG GAATAAGGAT 
GAGATCCGGT TCTGGCTGGA GAACAATGTG TCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissection of Glomerular Transcriptional Profile in Patients with Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function.
Diabetes(2017 Dec)
Pan Y,Jiang S,Hou Q,Qiu D,Shi J,Wang L,Chen Z,Zhang M,Duan A,Qin W,Zen K,Liu Z

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Multiplexed 3D FRET imaging in deep tissue of live embryos.
Scientific reports513991(2015 Sep)
Zhao M,Wan X,Li Y,Zhou W,Peng L

The Lowe syndrome protein OCRL1 is required for endocytosis in the zebrafish pronephric tubule.
PLoS genetics11(4)e1005058(2015 Apr)
Oltrabella F,Pietka G,Ramirez IB,Mironov A,Starborg T,Drummond IA,Hinchliffe KA,Lowe M

Transgenic labeling of the zebrafish pronephric duct and tubules using a promoter from the enpep gene.
Gene expression patterns : GEP11(1-2)118-21(2011 Jan-Feb)
Seiler C,Pack M

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enpep cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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