ddx42 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx42
Entrez Gene ID	503932
Full Name	DEAD (Asp-Glu-Ala-Asp) box helicase 42
Synonyms	im:7148194,si:dkey-15n1.10,zgc:111815
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box helicase 42 Names ATP-dependent RNA helicase DDX42 DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 dead box 42 protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037805.2	NP_001032894.2	ATP-dependent RNA helicase DDX42

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx42	NP_082350.3
Rattus norvegicus (Norway rat)	Ddx42	NP_001100529.1
Caenorhabditis elegans (roundworm)	C46F11.4	NP_497743.1
Canis lupus familiaris (dog)	DDX42	XP_537598.3
Danio rerio (zebrafish)	ddx42	NP_001032894.2
Homo sapiens (human)	DDX42	NP_031398.2
Pan troglodytes (chimpanzee)	DDX42	XP_001147880.1
Bos taurus (cattle)	DDX42	XP_003587506.2
Arabidopsis thaliana (thale cress)	AT2G47330	NP_566099.1
Xenopus tropicalis (tropical clawed frog)	ddx42	XP_004918655.1
	DDX42	XP_001116381.1
Drosophila melanogaster (fruit fly)	CG6418	NP_648413.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012523	XP_306246.4
Gallus gallus (chicken)	DDX42	NP_001026097.1
Oryza sativa (rice)	Os03g0308500	NP_001049904.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Fishing the molecular bases of Treacher Collins syndrome.
Weiner AM, Scampoli NL, Calcaterra NB
PloS one7(1)e29574(2012)

Identification and expression analysis of kcnh2 genes in the zebrafish.
Leong IU, Skinner JR, Shelling AN, Love DR
Biochemical and biophysical research communications396(4)817-24(2010 Jun)

Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo.
Lemeer S, Pinkse MW, Mohammed S, van Breukelen B, den Hertog J, Slijper M, Heck AJ
Journal of proteome research7(4)1555-64(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ddx42 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx42 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03673	NM_001037805.2 Latest version!	Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 42 (ddx42), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx42 ( NM_001037805.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001032894.2 Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 42 (ddx42), mRNA.

Price & Availability↑

CloneID	ODa03673
Clone ID Related Accession (Same CDS sequence)	NM_001037805.2
Accession Version	NM_001037805.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ATP-dependent RNA helicase DDX42
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX324157.4. On Aug 6, 2010 this sequence version replaced NM_001037805.1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01008467.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACTGGA ACAAGGGAGG CCCAAGTGGC AAGCGAGGGT TTGGATTTGG AGGATTCGCT 
CTTGCGACTG GGAAGAAAGA GGAGCCTCAT CTCCCACAGA AGGCCCACTC CTCTTTTGGG 
GGCACGGGAC CGTCAGGTGG ATATGGCAAG AATCAGCAGC TATCGTCGTT TTACAAAATA 
GGAACAAAAC GAGCCAATTT CGACGAGGAG AACGCTTACT TTGAGGATGA TGAAGAGGAG 
TCCACTGCTG ACCTGCCGTA TATACCCGCT GAGAACTCTC CCACTAGGAA GCAGTTCCAG 
TCTGGAGGGG GAGGAGGAGG TGGGGGCTCG GACAGTGAGG ATGACCCTCT TGATGCCTTT 
ATGGCTGAAG TGGAGGACCA GGCAGCAAAG GATATGAAAA AACTTGAGGA AAAGGAAAAA 
GAGAAAAAGA TGGCCAAGGG TATTCGGGAT GACATTGAAG AGGAAGATGA CCAAGAAGCA 
TATTTTCGCT ACATGGCGGA GAACCCAACA GCTGGGCTGA CCCAAGAGGA GGAGGAGGAA 
GAAGTGGACT ATGATAGTGA TGGAAACCCT ATTGCCCCTA CTACAAAAAA GATTATCATG 
CCCCTGCCCC CCATAGACCA CTCAGAGATT GACTATTCTC CATTTGAGAA GAACTTCTAT 
AATGAGCATG AGGAAATCAG CAGTTTGACT GGGGCCGAAG TGGTGGAACT GAGACGGAAA 
CTCAACTTAA AGGTTTCAGG CGCAGCCCCT CCTAAACCAG CAACCAGTTT TGCCCACTTT 
GGATTTGATG AACAGCTCAT GCATCAGATT CGTAAATCTG AGTACACTCA ACCGACCCCC 
ATCCAGTGCC AGGGCGTTCC TATAGCTTTG AGTGGCAGAG ATGCGATTGG CATTGCCAAA 
ACCGGTAGTG GAAAGACGGC AGCTTTCATT TGGCCAATTC TGGTGCACAT TATGGACCAG 
AAGGAGCTGG AGCCGGGAGA AGGGCCCATT GCTGTGATCG TCTGCCCCAC TAGAGAGCTC 
TGTCAACAGA TTCATGCTGA ATGCAAGCGC TTCGGGAAAG CATACGGCCT CCGCTCAGTG 
GCAGTATATG GAGGTGGCAG CATGTGGGAA CAAGCCAAAG CCCTACAGGA AGGAGCTGAA 
ATTGTTGTCT GCACCCCAGG CCGTCTGATC GATCATGTAA AAAAGAAAGC TACGTCTCTG 
CAGCGAGTGA CCTTTCTAGT ATTTGATGAA GCTGATCGCA TGTTTGATAT GGGCTTTGAA 
TACCAAGTGA GATCCATTGC CAGCCATGTT CGACCTGATC GACAAACTCT GTTATTCAGT 
GCAACTTTCC GGAAGAAAAT CGAAAAACTA GCACGAGATA TTTTGGTCGA CCCCATTCGT 
GTGGTGCAGG GAGACATAGG AGAGGCTAAT GAAGACATCA CTCAGATAGT GGAGGTGCTG 
CAGTCTGGCC AGGACAAATG GGGTTGGCTG ACCCGCAGAC TGGTGGAGTT CACATCCGCT 
GGCTCAGTGC TGGTGTTTGT TACTAAAAAG GCTAACTGTG AGGAGCTGGC AACAAATTTA 
ATTCAGGAAG GATATAGCCT GGGTCTGCTT CATGGAGACA TGGATCAGAG TGAGAGGAAC 
AAAGTCATAG CAGACTTTAA AAAGAAGAAT CTGCCTGTAT TGGTGGCGAC CGATGTTGCT 
GCTCGTGGGT TGGATATTCC ATCTATTCGC ACTGTTGTCA ACTATGATGT AGCCCGGGAC 
ATTGACACAC ATACACATCG AATTGGTAGA ACAGGTCGTG CTGGAGAAAA AGGTGTAGCT 
TATACATTGC TCACCACTAA AGACACTTCA TTTGCCGGTG ACCTGGTTCG TAATTTAGAG 
GGAGCCAATC AGAGTGTTTC AAAAGATCTG TTAGACCTGG CTATGCAGAA TCCCTGGTTC 
AGGAAATCTC GCTTTAAGGG AGGAAAAGGG AAGAAGATTA ATATTGGAGG TGGAGGCTTG 
GGCTATAGAG AGAGACCTGG CCTTGGATCG GAATGCTCTG AGCGCAGTGG TGGTTCTATT 
GCAGCAGCTC TTGGCAACTA TGAAACCTTC AAACCCTCCA CTGGAGCAAT GGGAGACCGT 
ATGTCTGCCT TAAAGCAAGC ATTTCAGGCT CAGTACAAAA ACCACTTTGT CGCGGCATCA 
GGTGTGCCTC CTAAACTCAC CACAAAGTCC AGCAGCTCAT CTGGATGGAC CAGCGCTGGA 
AGTCTGAGTT CTTTGCCAAC AGGAGCACCT GAGGGGCCGG ACAGACCTCA CCTGTCCTTC 
TCGCCATCAT CCTCTTCCTC GTTTTCCATG CCACCGCCTC CAGCTCTCAC CTCAGGCATT 
AAAATGAGTG GCTTCAGCAG TGCGGGCACC CTCAGCTCAA TTTCAGACTC TTACTCTAGC 
TCTTCTTCAA AAGAGCCGTC CCGTAGCGAG AGGCACTCTG ATGACAGGGG CCGGCATGGA 
GATAGTCATA GTCACCGTCA TAGTGACAGG CATAGTGGAG GAGAGCGAGA ACGAGAGAGA 
GAGCGTGATC GTGACCGCGA TCGCCACGGG GAAAGGGACC GCCATAGTGA CAGAGATCGG 
CATGGGGATA GCCGAAATGG TGAAGGGAGC CATAGGGACA GAGAGGGACG GTCAGAAAGA 
GATGAAGGTG AGAAGTCAGG GTCTCACAAA GAAAGCTTTG CAGTTCCTGA TCCTCCGAAA 
CGTAAGAAAA GCAGATGGGA CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032894.2
CDS	131..2857
Translation	MNWNKGGPSGKRGFGFGGFALATGKKEEPHLPQKAHSSFGGTGPSGGYGKNQQLSSFYKIGTKRANFDEENAYFEDDEEESTADLPYIPAENSPTRKQFQSGGGGGGGGSDSEDDPLDAFMAEVEDQAAKDMKKLEEKEKEKKMAKGIRDDIEEEDDQEAYFRYMAENPTAGLTQEEEEEEVDYDSDGNPIAPTTKKIIMPLPPIDHSEIDYSPFEKNFYNEHEEISSLTGAEVVELRRKLNLKVSGAAPPKPATSFAHFGFDEQLMHQIRKSEYTQPTPIQCQGVPIALSGRDAIGIAKTGSGKTAAFIWPILVHIMDQKELEPGEGPIAVIVCPTRELCQQIHAECKRFGKAYGLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATSLQRVTFLVFDEADRMFDMGFEYQVRSIASHVRPDRQTLLFSATFRKKIEKLARDILVDPIRVVQGDIGEANEDITQIVEVLQSGQDKWGWLTRRLVEFTSAGSVLVFVTKKANCEELATNLIQEGYSLGLLHGDMDQSERNKVIADFKKKNLPVLVATDVAARGLDIPSIRTVVNYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTTKDTSFAGDLVRNLEGANQSVSKDLLDLAMQNPWFRKSRFKGGKGKKINIGGGGLGYRERPGLGSECSERSGGSIAAALGNYETFKPSTGAMGDRMSALKQAFQAQYKNHFVAASGVPPKLTTKSSSSSGWTSAGSLSSLPTGAPEGPDRPHLSFSPSSSSSFSMPPPPALTSGIKMSGFSSAGTLSSISDSYSSSSSKEPSRSERHSDDRGRHGDSHSHRHSDRHSGGERERERERDRDRDRHGERDRHSDRDRHGDSRNGEGSHRDREGRSERDEGEKSGSHKESFAVPDPPKRKKSRWDN

Target ORF information:

RefSeq Version	NM_001037805.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DEAD (Asp-Glu-Ala-Asp) box helicase 42 (ddx42), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037805.2

ORF Insert Sequence:

ATGAACTGGA ACAAGGGAGG CCCAAGTGGC AAGCGAGGGT TTGGATTTGG AGGATTCGCT 
CTTGCGACTG GGAAGAAAGA GGAGCCTCAT CTCCCACAGA AGGCCCACTC CTCTTTTGGG 
GGCACGGGAC CGTCAGGTGG ATATGGCAAG AATCAGCAGC TATCGTCGTT TTACAAAATA 
GGAACAAAAC GAGCCAATTT CGACGAGGAG AACGCTTACT TTGAGGATGA TGAAGAGGAG 
TCCACTGCTG ACCTGCCGTA TATACCCGCT GAGAACTCTC CCACTAGGAA GCAGTTCCAG 
TCTGGAGGGG GAGGAGGAGG TGGGGGCTCG GACAGTGAGG ATGACCCTCT TGATGCCTTT 
ATGGCTGAAG TGGAGGACCA GGCAGCAAAG GATATGAAAA AACTTGAGGA AAAGGAAAAA 
GAGAAAAAGA TGGCCAAGGG TATTCGGGAT GACATTGAAG AGGAAGATGA CCAAGAAGCA 
TATTTTCGCT ACATGGCGGA GAACCCAACA GCTGGGCTGA CCCAAGAGGA GGAGGAGGAA 
GAAGTGGACT ATGATAGTGA TGGAAACCCT ATTGCCCCTA CTACAAAAAA GATTATCATG 
CCCCTGCCCC CCATAGACCA CTCAGAGATT GACTATTCTC CATTTGAGAA GAACTTCTAT 
AATGAGCATG AGGAAATCAG CAGTTTGACT GGGGCCGAAG TGGTGGAACT GAGACGGAAA 
CTCAACTTAA AGGTTTCAGG CGCAGCCCCT CCTAAACCAG CAACCAGTTT TGCCCACTTT 
GGATTTGATG AACAGCTCAT GCATCAGATT CGTAAATCTG AGTACACTCA ACCGACCCCC 
ATCCAGTGCC AGGGCGTTCC TATAGCTTTG AGTGGCAGAG ATGCGATTGG CATTGCCAAA 
ACCGGTAGTG GAAAGACGGC AGCTTTCATT TGGCCAATTC TGGTGCACAT TATGGACCAG 
AAGGAGCTGG AGCCGGGAGA AGGGCCCATT GCTGTGATCG TCTGCCCCAC TAGAGAGCTC 
TGTCAACAGA TTCATGCTGA ATGCAAGCGC TTCGGGAAAG CATACGGCCT CCGCTCAGTG 
GCAGTATATG GAGGTGGCAG CATGTGGGAA CAAGCCAAAG CCCTACAGGA AGGAGCTGAA 
ATTGTTGTCT GCACCCCAGG CCGTCTGATC GATCATGTAA AAAAGAAAGC TACGTCTCTG 
CAGCGAGTGA CCTTTCTAGT ATTTGATGAA GCTGATCGCA TGTTTGATAT GGGCTTTGAA 
TACCAAGTGA GATCCATTGC CAGCCATGTT CGACCTGATC GACAAACTCT GTTATTCAGT 
GCAACTTTCC GGAAGAAAAT CGAAAAACTA GCACGAGATA TTTTGGTCGA CCCCATTCGT 
GTGGTGCAGG GAGACATAGG AGAGGCTAAT GAAGACATCA CTCAGATAGT GGAGGTGCTG 
CAGTCTGGCC AGGACAAATG GGGTTGGCTG ACCCGCAGAC TGGTGGAGTT CACATCCGCT 
GGCTCAGTGC TGGTGTTTGT TACTAAAAAG GCTAACTGTG AGGAGCTGGC AACAAATTTA 
ATTCAGGAAG GATATAGCCT GGGTCTGCTT CATGGAGACA TGGATCAGAG TGAGAGGAAC 
AAAGTCATAG CAGACTTTAA AAAGAAGAAT CTGCCTGTAT TGGTGGCGAC CGATGTTGCT 
GCTCGTGGGT TGGATATTCC ATCTATTCGC ACTGTTGTCA ACTATGATGT AGCCCGGGAC 
ATTGACACAC ATACACATCG AATTGGTAGA ACAGGTCGTG CTGGAGAAAA AGGTGTAGCT 
TATACATTGC TCACCACTAA AGACACTTCA TTTGCCGGTG ACCTGGTTCG TAATTTAGAG 
GGAGCCAATC AGAGTGTTTC AAAAGATCTG TTAGACCTGG CTATGCAGAA TCCCTGGTTC 
AGGAAATCTC GCTTTAAGGG AGGAAAAGGG AAGAAGATTA ATATTGGAGG TGGAGGCTTG 
GGCTATAGAG AGAGACCTGG CCTTGGATCG GAATGCTCTG AGCGCAGTGG TGGTTCTATT 
GCAGCAGCTC TTGGCAACTA TGAAACCTTC AAACCCTCCA CTGGAGCAAT GGGAGACCGT 
ATGTCTGCCT TAAAGCAAGC ATTTCAGGCT CAGTACAAAA ACCACTTTGT CGCGGCATCA 
GGTGTGCCTC CTAAACTCAC CACAAAGTCC AGCAGCTCAT CTGGATGGAC CAGCGCTGGA 
AGTCTGAGTT CTTTGCCAAC AGGAGCACCT GAGGGGCCGG ACAGACCTCA CCTGTCCTTC 
TCGCCATCAT CCTCTTCCTC GTTTTCCATG CCACCGCCTC CAGCTCTCAC CTCAGGCATT 
AAAATGAGTG GCTTCAGCAG TGCGGGCACC CTCAGCTCAA TTTCAGACTC TTACTCTAGC 
TCTTCTTCAA AAGAGCCGTC CCGTAGCGAG AGGCACTCTG ATGACAGGGG CCGGCATGGA 
GATAGTCATA GTCACCGTCA TAGTGACAGG CATAGTGGAG GAGAGCGAGA ACGAGAGAGA 
GAGCGTGATC GTGACCGCGA TCGCCACGGG GAAAGGGACC GCCATAGTGA CAGAGATCGG 
CATGGGGATA GCCGAAATGG TGAAGGGAGC CATAGGGACA GAGAGGGACG GTCAGAAAGA 
GATGAAGGTG AGAAGTCAGG GTCTCACAAA GAAAGCTTTG CAGTTCCTGA TCCTCCGAAA 
CGTAAGAAAA GCAGATGGGA CAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Fishing the molecular bases of Treacher Collins syndrome.
PloS one7(1)e29574(2012)
Weiner AM,Scampoli NL,Calcaterra NB

Identification and expression analysis of kcnh2 genes in the zebrafish.
Biochemical and biophysical research communications396(4)817-24(2010 Jun)
Leong IU,Skinner JR,Shelling AN,Love DR

Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo.
Journal of proteome research7(4)1555-64(2008 Apr)
Lemeer S,Pinkse MW,Mohammed S,van Breukelen B,den Hertog J,Slijper M,Heck AJ

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ddx42 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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