col2a1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	col2a1b
Entrez Gene ID	503730
Full Name	collagen, type II, alpha 1b
Synonyms	im:6788778
General protein information	Preferred Names collagen, type II, alpha 1b Names collagen, type II, alpha 1b zmp:0000000538
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281478.1	NP_001268407.1	collagen, type II, alpha 1b precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Damage-induced reactive oxygen species regulate
LeBert D, Squirrell JM, Freisinger C, Rindy J, Golenberg N, Frecentes G, Gibson A, Eliceiri KW, Huttenlocher A
eLife7(2018 Jan)

Loss of DDRGK1 modulates SOX9 ubiquitination in spondyloepimetaphyseal dysplasia.
Egunsola AT, Bae Y, Jiang MM, Liu DS, Chen-Evenson Y, Bertin T, Chen S, Lu JT, Nevarez L, Magal N, Raas-Rothschild A, Swindell EC, Cohn DH, Gibbs RA, Campeau PM, Shohat M, Lee BH
The Journal of clinical investigation127(4)1475-1484(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that both col1a1a and col2a1b were found in actinotrichia forming cells.
Title: Actinotrichia collagens and their role in fin formation.

The following col2a1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col2a1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02189	NM_001281478.1 Latest version!	Danio rerio collagen, type II, alpha 1b (col2a1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col2a1b ( NM_001281478.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001268407.1 Danio rerio collagen, type II, alpha 1b (col2a1b), mRNA.

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CloneID	ODa02189
Clone ID Related Accession (Same CDS sequence)	NM_001281478.1
Accession Version	NM_001281478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	collagen, type II, alpha 1b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from HF563617.1. On Aug 31, 2013 this sequence version replaced XM_005172758.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HF563615.1, HF563616.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505392, SAMEA3505393 [ECO:0000350] ##Evidence-Data-END##

ATGATGAAGA CCTCCATGAT GGTGCGGAGC GCGGAGCTGC TGCTGTGTGT GGCCGCGCTG 
GGAGCTCTGC TGACCGGGGG AGCAGCGCAG GATGAGGAGC AAGACCCCGG CGGCTGCTCT 
CAGGATGGTC AGCTGTACCG GGATAAGGAC GTGTGGAAGC CGGAGCCGTG CCGGATCTGT 
GTGTGTGACA GCGGCACCGT GCTGTGCGAT GAGATCGTGT GTGAGGAGCT GAGGGACTGC 
GCTAAACCCG AAATCCCGCT CGGGGAGTGC TGTCCCGTCT GCGCCAGCGC CGACGCCTCC 
ACACCTGAGC GCCTGCCGGG TGCCAAGGGA CAAAAGGGGG AACCAGGAGA CATAACTGAT 
GTTGTGGGGC CGCGGGGACC GTCCGGGCCG ATGGGTCCGC CGGGAGAACA AGGCCCACGC 
GGAGACCGGG GAGAGAAGGG GGAGAAGGGA AGTCCGGGCC CTCGGGGACG AGATGGAGAG 
CCGGGAACCC CAGGAAACCC TGGACCTCCA GGACCTCCAG GACCCAACGG ACCACCAGGC 
CTTGGCGGAA ATTTCGCATC TCAGATGGCG GGAGGATTTG ATGACAAAGC GGGAGCGGCT 
CAGATGGGGG TGATGCAGGG CCCGATGGGT CCGATGGGCC CCCGAGGACC CCCAGGCCCC 
AACGGAGCTC CGGGTCCTCA AGGATTCCAA GGAAACCCAG GAGAAGGCGG AGAGCCGGGA 
TCATCCGGCC CAATGGGTCC CCGTGGCCCA CCCGGACCCC CAGGGAAACC AGGAGATGAT 
GGTGAGCCGG GCAAACCTGG CAACGGTGGA GAGCGTGGAC CCCCAGGCCC TCAGGGAGCT 
CGTGGATTCC CAGGAACACC CGGACTACCT GGAATCAAGG GACACAGAGG TTACACAGGT 
CTTGATGGTG CTAAAGGAGA GTCTGGAGCT ACTGGAGCAA AAGGTGAATC CGGCTCTCCT 
GGAGAAAGTG GAGCTCCTGG ACCGATGGGT CCGCGAGGTC TGCCTGGTGA ACGGGGGCGT 
CCAGGACCGT CAGGAGCTTC TGGTGCTCGT GGTAATGACG GTCTGCCTGG CGGTGCTGGT 
CCACCGGGTC CCGTTGGTAC AGCAGGATCT CCCGGGTTCC CAGGATCCCC CGGTGCAAAG 
GGTGAAGCGG GACCAACCGG AGCCCGAGGG CCTGAGGGAG CACAGGGGCC CCGTGGAGAG 
TCTGGTGTTC CTGGAGCCTC GGGACCCTCT GGAGTCTCTG GTAACCCTGG TTCTGACGGG 
ATGCCTGGAG CCAAAGGATC AGTGGGCGCT CCGGGTATCG GTGGAGCTCC TGGTTTCCCG 
GGTCCGCGTG GTCCTCCAGG CCCTCAGGGA GCGACAGGTC CTCTAGGGCC AAAGGGTCAA 
TCTGGGGACT CGGGCCTCGC AGGGTTCAAA GGTGAAGCCG GTCCTAAAGG AGAGATTGGA 
AATGCTGGTC TACAAGGGGC TCCAGGTCCT GCTGGAGAAG AAGGCAAGCG AGGACCCAGA 
GGAGAGCCTG GTGCTGCGGG CCCCCCAGGG CCAACTGGAG AACGGGGAAC GCCTGGTAAT 
CGAGGTTTTC CCGGACAAGA CGGTCTTGCT GGTCCAAAGG GTGCGCCCGG AGAGCGTGGA 
CCTGCAGGCG TATCGGGACC TAAAGGAGCC GGTGGAGATC CAGGGCGGCC GGGAGAACCA 
GGTCTACCTG GTGCAAGGGG TTTGACGGGA CGTCCAGGAG ACGCCGGTCC ACAGGGCAAA 
GTCGGTCCGT CTGGCGCTCC GGGTGAAGAC GGTCGTCCGG GACCCCCGGG GCCTCAGGGT 
GTTCGCGGTC AGCCAGGAGT CATGGGATTC CCGGGACCCA AGGGTGGAAA TGGTGAGGCT 
GGAAAGGCTG GAGAGAAGGG ACTCGCTGGA GCTCCGGGTC TGCGTGGTCT GCCTGGTAAA 
GATGGAGAGA CCGGTGCTGC TGGGCCTCCA GGACCTGCCG GCTCTGCGGG AGAGCGAGGC 
GAGCAGGGAC AGCCGGGACC ATCAGGCTTC CAGGGTCTGC CTGGACCCCC GGGGCCCCCC 
GGTGAGGGAG GTAAACCTGG AGATCAGGGA GTTCCTGGAG AGGCCGGAGG GGCAGGGGCT 
ACCGGACCCC GAGGTGAGCG AGGGTTCCCA GGAGAGAGAG GCGGTGCTGG GCCACAGGGG 
CTCCAGGGGC CCCGGGGTCT ACCAGGAACA CCAGGAACCG ACGGCCCCAA GGGTGGTGTG 
GGACCCGCGG GGACAGCTGG AGCTCAGGGT CCTCCAGGAC TGCAGGGAAT GCCCGGAGAG 
AGAGGCACGT CTGGGAATCC AGGACCAAAG GGCGACCGAG GCGATAATGG AGATAAGGGA 
CCAGAAGGAG CTCCGGGTAA AGACGGCTCA AGAGGTTTAA CGGGTCCAAT TGGTCCAACA 
GGTCCTGCGG GACCCAACGG TGAAAAGGGA GAATCGGGAC CCGCGGGACC GTCTGGAGTT 
GCGGGGACTC GCGGTGTTCC TGGTGACCGA GGAGAGACTG GCCCTCCTGG TCCTGCTGGT 
TTTGCTGGTC CACCAGGTGC AGATGGACAG CCGGGAGTGA AAGGAGAGCA GGGTGAAGGT 
GGACAGAAGG GTGATGCTGG AGCCCCGGGC CCTCAGGGGC CCTCTGGAGC TCCGGGCCCG 
CAGGGACCAA CCGGTGTGTC TGGACCAAAA GGGGCTCGTG GTGCTCAAGG CCCTCCTGGT 
GCCACGGGTT TCCCCGGAGC CGCTGGAAGA GTTGGACCTC CTGGTCCTAA TGGTAACCCT 
GGTCCAGCCG GTCCTGCTGG TCCTCCTGGT AAAGATGGGC CAAAGGGTGT CAGAGGAGAT 
GGTGGTCCTC CTGGAAGACC TGGAGACGCT GGTTTACGGG GTTCGGCTGG TCCTGCAGGA 
GAGAAGGGAG ATCCTGGTGA AGACGGTCCT CACGGTCCGG ACGGTCCCGC AGGTCCCCAG 
GGTCTGGCCG GTCAGCGCGG TATCGTTGGT CTTCCAGGAC AAAGAGGCGA AAGAGGATTC 
CCCGGCCTGC CTGGACCCTC CGGTGAGCCG GGTAAGCAGG GGGCTCCTGG GGGACCCGGG 
GACAGAGGAC CTCCTGGCCC TGTAGGGGCA CCAGGTCTGA CCGGCGCTGC AGGAGAGCCG 
GGGAGAGAGG GCAATCCTGG ATCTGATGGT CCTCCTGGTC GAGATGGTTC TGCTGGAATA 
AAGGGAGACC GAGGTGACAC GGGTCCAGCT GGAGCTCCTG GTGCTCCAGG GGGTCCTGGT 
GCTCCTGGTC CAGTCGGACC CACTGGTAAA CAGGGAGACA GAGGAGAGGC GGGTCCCCAT 
GGGCCTTCTG GACCTCCAGG GCCTGCTGGA GCTCGGGGAA TGCCTGGACC ACAAGGACCG 
CGTGGAGATA AAGGAGAAGG TGGGGATTCG GGAGATAGAG GGCAGAAAGG ACACAGAGGC 
TTCACTGGTC TCCAAGGTCT GCCTGGGTCT CCTGGTCAAC CTGGTGATCA AGGTGCTTCT 
GGACCTTCTG GACCCGGTGG AGCAAGAGGA CCCCCAGGCC CTGTCGGTCC AGCTGGAAAA 
GATGGAGCAA ACGGTTTACC TGGACCCATT GGACCCCCGG GACCCCGTGG TCGTTCTGGG 
GAGACTGGTC CATCAGGTCC TCCTGGAACT CCTGGACCCC CTGGTCCTCC TGGCCCTCCG 
GGACCTGGCA TCGACATGTC AGCCTTTGCT GGTCTGTCTC AGCTTGAGAA GTCTCCTGAT 
CCCCTGAGGT ACATGAGGGC AGACCAGGCC GCAGATGGTA ACCATCAGCA TGATGCAGAG 
GTGGATGCCA CGCTCAAATC CCTGAACAAC CAGATGGAGA ACATCCGCAG GCCTGATGGC 
ACCAAAAAGA GCCCGGCCCG GACCTGCAGA GACCTGAAGC AGTGCCACCC AGACTGGAAG 
AGCGGCGAAT ACTGGATTGA CCCGAACCAG GGCTGCACCG TCGATGCTAT CAAAGTGTTC 
TGCAACATGG AGACCGGCGA GAGCTGCATT TACCCCAAAC CTGCCAACAT CCCACGCAAG 
AACTGGTGGA CTACGAAGGG TGGGGATCGC AAACACATTT GGTTTGGGGA AGCCATGAAT 
GGAGGATTCC ACTTTAACTA TGGAGACGAG AATCTGTCGC CAAATACAGC CAGTATTCAG 
ATGACCTTCC TCAGACTGCT GTCCACTGAA GCCTCGCAGA ACCTTACCTA CCACTGCAGG 
AACAGCATCG CCTACATGGA CGAATCCTCT GGGAACCTGA AGAAGGCTCT GCTGCTGCGC 
GGATCCAATG ATGTGGAGAT CCGGGCGGAA GGAAACAGCC GCTTCACCTA CAGCGTTCTG 
GAGGACGGAT GCAAGAAACA CACAGGACAG TGGAGCAAGA CCGTCATTGA GTATAAATCC 
CAGAAGACGT CTCGCCTGCC CCTTGTGGAC ATTGCCCCTG TGGATATAGG AGGAGCACAT 
CAGGAGTTCG GAGTGGATGT AGGAGCCGTC TGTTTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268407.1
CDS	60..4541
Translation	MMKTSMMVRSAELLLCVAALGALLTGGAAQDEEQDPGGCSQDGQLYRDKDVWKPEPCRICVCDSGTVLCDEIVCEELRDCAKPEIPLGECCPVCASADASTPERLPGAKGQKGEPGDITDVVGPRGPSGPMGPPGEQGPRGDRGEKGEKGSPGPRGRDGEPGTPGNPGPPGPPGPNGPPGLGGNFASQMAGGFDDKAGAAQMGVMQGPMGPMGPRGPPGPNGAPGPQGFQGNPGEGGEPGSSGPMGPRGPPGPPGKPGDDGEPGKPGNGGERGPPGPQGARGFPGTPGLPGIKGHRGYTGLDGAKGESGATGAKGESGSPGESGAPGPMGPRGLPGERGRPGPSGASGARGNDGLPGGAGPPGPVGTAGSPGFPGSPGAKGEAGPTGARGPEGAQGPRGESGVPGASGPSGVSGNPGSDGMPGAKGSVGAPGIGGAPGFPGPRGPPGPQGATGPLGPKGQSGDSGLAGFKGEAGPKGEIGNAGLQGAPGPAGEEGKRGPRGEPGAAGPPGPTGERGTPGNRGFPGQDGLAGPKGAPGERGPAGVSGPKGAGGDPGRPGEPGLPGARGLTGRPGDAGPQGKVGPSGAPGEDGRPGPPGPQGVRGQPGVMGFPGPKGGNGEAGKAGEKGLAGAPGLRGLPGKDGETGAAGPPGPAGSAGERGEQGQPGPSGFQGLPGPPGPPGEGGKPGDQGVPGEAGGAGATGPRGERGFPGERGGAGPQGLQGPRGLPGTPGTDGPKGGVGPAGTAGAQGPPGLQGMPGERGTSGNPGPKGDRGDNGDKGPEGAPGKDGSRGLTGPIGPTGPAGPNGEKGESGPAGPSGVAGTRGVPGDRGETGPPGPAGFAGPPGADGQPGVKGEQGEGGQKGDAGAPGPQGPSGAPGPQGPTGVSGPKGARGAQGPPGATGFPGAAGRVGPPGPNGNPGPAGPAGPPGKDGPKGVRGDGGPPGRPGDAGLRGSAGPAGEKGDPGEDGPHGPDGPAGPQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGGPGDRGPPGPVGAPGLTGAAGEPGREGNPGSDGPPGRDGSAGIKGDRGDTGPAGAPGAPGGPGAPGPVGPTGKQGDRGEAGPHGPSGPPGPAGARGMPGPQGPRGDKGEGGDSGDRGQKGHRGFTGLQGLPGSPGQPGDQGASGPSGPGGARGPPGPVGPAGKDGANGLPGPIGPPGPRGRSGETGPSGPPGTPGPPGPPGPPGPGIDMSAFAGLSQLEKSPDPLRYMRADQAADGNHQHDAEVDATLKSLNNQMENIRRPDGTKKSPARTCRDLKQCHPDWKSGEYWIDPNQGCTVDAIKVFCNMETGESCIYPKPANIPRKNWWTTKGGDRKHIWFGEAMNGGFHFNYGDENLSPNTASIQMTFLRLLSTEASQNLTYHCRNSIAYMDESSGNLKKALLLRGSNDVEIRAEGNSRFTYSVLEDGCKKHTGQWSKTVIEYKSQKTSRLPLVDIAPVDIGGAHQEFGVDVGAVCFL

Target ORF information:

RefSeq Version	NM_001281478.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio collagen, type II, alpha 1b (col2a1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281478.1

ORF Insert Sequence:

ATGATGAAGA CCTCCATGAT GGTGCGGAGC GCGGAGCTGC TGCTGTGTGT GGCCGCGCTG 
GGAGCTCTGC TGACCGGGGG AGCAGCGCAG GATGAGGAGC AAGACCCCGG CGGCTGCTCT 
CAGGATGGTC AGCTGTACCG GGATAAGGAC GTGTGGAAGC CGGAGCCGTG CCGGATCTGT 
GTGTGTGACA GCGGCACCGT GCTGTGCGAT GAGATCGTGT GTGAGGAGCT GAGGGACTGC 
GCTAAACCCG AAATCCCGCT CGGGGAGTGC TGTCCCGTCT GCGCCAGCGC CGACGCCTCC 
ACACCTGAGC GCCTGCCGGG TGCCAAGGGA CAAAAGGGGG AACCAGGAGA CATAACTGAT 
GTTGTGGGGC CGCGGGGACC GTCCGGGCCG ATGGGTCCGC CGGGAGAACA AGGCCCACGC 
GGAGACCGGG GAGAGAAGGG GGAGAAGGGA AGTCCGGGCC CTCGGGGACG AGATGGAGAG 
CCGGGAACCC CAGGAAACCC TGGACCTCCA GGACCTCCAG GACCCAACGG ACCACCAGGC 
CTTGGCGGAA ATTTCGCATC TCAGATGGCG GGAGGATTTG ATGACAAAGC GGGAGCGGCT 
CAGATGGGGG TGATGCAGGG CCCGATGGGT CCGATGGGCC CCCGAGGACC CCCAGGCCCC 
AACGGAGCTC CGGGTCCTCA AGGATTCCAA GGAAACCCAG GAGAAGGCGG AGAGCCGGGA 
TCATCCGGCC CAATGGGTCC CCGTGGCCCA CCCGGACCCC CAGGGAAACC AGGAGATGAT 
GGTGAGCCGG GCAAACCTGG CAACGGTGGA GAGCGTGGAC CCCCAGGCCC TCAGGGAGCT 
CGTGGATTCC CAGGAACACC CGGACTACCT GGAATCAAGG GACACAGAGG TTACACAGGT 
CTTGATGGTG CTAAAGGAGA GTCTGGAGCT ACTGGAGCAA AAGGTGAATC CGGCTCTCCT 
GGAGAAAGTG GAGCTCCTGG ACCGATGGGT CCGCGAGGTC TGCCTGGTGA ACGGGGGCGT 
CCAGGACCGT CAGGAGCTTC TGGTGCTCGT GGTAATGACG GTCTGCCTGG CGGTGCTGGT 
CCACCGGGTC CCGTTGGTAC AGCAGGATCT CCCGGGTTCC CAGGATCCCC CGGTGCAAAG 
GGTGAAGCGG GACCAACCGG AGCCCGAGGG CCTGAGGGAG CACAGGGGCC CCGTGGAGAG 
TCTGGTGTTC CTGGAGCCTC GGGACCCTCT GGAGTCTCTG GTAACCCTGG TTCTGACGGG 
ATGCCTGGAG CCAAAGGATC AGTGGGCGCT CCGGGTATCG GTGGAGCTCC TGGTTTCCCG 
GGTCCGCGTG GTCCTCCAGG CCCTCAGGGA GCGACAGGTC CTCTAGGGCC AAAGGGTCAA 
TCTGGGGACT CGGGCCTCGC AGGGTTCAAA GGTGAAGCCG GTCCTAAAGG AGAGATTGGA 
AATGCTGGTC TACAAGGGGC TCCAGGTCCT GCTGGAGAAG AAGGCAAGCG AGGACCCAGA 
GGAGAGCCTG GTGCTGCGGG CCCCCCAGGG CCAACTGGAG AACGGGGAAC GCCTGGTAAT 
CGAGGTTTTC CCGGACAAGA CGGTCTTGCT GGTCCAAAGG GTGCGCCCGG AGAGCGTGGA 
CCTGCAGGCG TATCGGGACC TAAAGGAGCC GGTGGAGATC CAGGGCGGCC GGGAGAACCA 
GGTCTACCTG GTGCAAGGGG TTTGACGGGA CGTCCAGGAG ACGCCGGTCC ACAGGGCAAA 
GTCGGTCCGT CTGGCGCTCC GGGTGAAGAC GGTCGTCCGG GACCCCCGGG GCCTCAGGGT 
GTTCGCGGTC AGCCAGGAGT CATGGGATTC CCGGGACCCA AGGGTGGAAA TGGTGAGGCT 
GGAAAGGCTG GAGAGAAGGG ACTCGCTGGA GCTCCGGGTC TGCGTGGTCT GCCTGGTAAA 
GATGGAGAGA CCGGTGCTGC TGGGCCTCCA GGACCTGCCG GCTCTGCGGG AGAGCGAGGC 
GAGCAGGGAC AGCCGGGACC ATCAGGCTTC CAGGGTCTGC CTGGACCCCC GGGGCCCCCC 
GGTGAGGGAG GTAAACCTGG AGATCAGGGA GTTCCTGGAG AGGCCGGAGG GGCAGGGGCT 
ACCGGACCCC GAGGTGAGCG AGGGTTCCCA GGAGAGAGAG GCGGTGCTGG GCCACAGGGG 
CTCCAGGGGC CCCGGGGTCT ACCAGGAACA CCAGGAACCG ACGGCCCCAA GGGTGGTGTG 
GGACCCGCGG GGACAGCTGG AGCTCAGGGT CCTCCAGGAC TGCAGGGAAT GCCCGGAGAG 
AGAGGCACGT CTGGGAATCC AGGACCAAAG GGCGACCGAG GCGATAATGG AGATAAGGGA 
CCAGAAGGAG CTCCGGGTAA AGACGGCTCA AGAGGTTTAA CGGGTCCAAT TGGTCCAACA 
GGTCCTGCGG GACCCAACGG TGAAAAGGGA GAATCGGGAC CCGCGGGACC GTCTGGAGTT 
GCGGGGACTC GCGGTGTTCC TGGTGACCGA GGAGAGACTG GCCCTCCTGG TCCTGCTGGT 
TTTGCTGGTC CACCAGGTGC AGATGGACAG CCGGGAGTGA AAGGAGAGCA GGGTGAAGGT 
GGACAGAAGG GTGATGCTGG AGCCCCGGGC CCTCAGGGGC CCTCTGGAGC TCCGGGCCCG 
CAGGGACCAA CCGGTGTGTC TGGACCAAAA GGGGCTCGTG GTGCTCAAGG CCCTCCTGGT 
GCCACGGGTT TCCCCGGAGC CGCTGGAAGA GTTGGACCTC CTGGTCCTAA TGGTAACCCT 
GGTCCAGCCG GTCCTGCTGG TCCTCCTGGT AAAGATGGGC CAAAGGGTGT CAGAGGAGAT 
GGTGGTCCTC CTGGAAGACC TGGAGACGCT GGTTTACGGG GTTCGGCTGG TCCTGCAGGA 
GAGAAGGGAG ATCCTGGTGA AGACGGTCCT CACGGTCCGG ACGGTCCCGC AGGTCCCCAG 
GGTCTGGCCG GTCAGCGCGG TATCGTTGGT CTTCCAGGAC AAAGAGGCGA AAGAGGATTC 
CCCGGCCTGC CTGGACCCTC CGGTGAGCCG GGTAAGCAGG GGGCTCCTGG GGGACCCGGG 
GACAGAGGAC CTCCTGGCCC TGTAGGGGCA CCAGGTCTGA CCGGCGCTGC AGGAGAGCCG 
GGGAGAGAGG GCAATCCTGG ATCTGATGGT CCTCCTGGTC GAGATGGTTC TGCTGGAATA 
AAGGGAGACC GAGGTGACAC GGGTCCAGCT GGAGCTCCTG GTGCTCCAGG GGGTCCTGGT 
GCTCCTGGTC CAGTCGGACC CACTGGTAAA CAGGGAGACA GAGGAGAGGC GGGTCCCCAT 
GGGCCTTCTG GACCTCCAGG GCCTGCTGGA GCTCGGGGAA TGCCTGGACC ACAAGGACCG 
CGTGGAGATA AAGGAGAAGG TGGGGATTCG GGAGATAGAG GGCAGAAAGG ACACAGAGGC 
TTCACTGGTC TCCAAGGTCT GCCTGGGTCT CCTGGTCAAC CTGGTGATCA AGGTGCTTCT 
GGACCTTCTG GACCCGGTGG AGCAAGAGGA CCCCCAGGCC CTGTCGGTCC AGCTGGAAAA 
GATGGAGCAA ACGGTTTACC TGGACCCATT GGACCCCCGG GACCCCGTGG TCGTTCTGGG 
GAGACTGGTC CATCAGGTCC TCCTGGAACT CCTGGACCCC CTGGTCCTCC TGGCCCTCCG 
GGACCTGGCA TCGACATGTC AGCCTTTGCT GGTCTGTCTC AGCTTGAGAA GTCTCCTGAT 
CCCCTGAGGT ACATGAGGGC AGACCAGGCC GCAGATGGTA ACCATCAGCA TGATGCAGAG 
GTGGATGCCA CGCTCAAATC CCTGAACAAC CAGATGGAGA ACATCCGCAG GCCTGATGGC 
ACCAAAAAGA GCCCGGCCCG GACCTGCAGA GACCTGAAGC AGTGCCACCC AGACTGGAAG 
AGCGGCGAAT ACTGGATTGA CCCGAACCAG GGCTGCACCG TCGATGCTAT CAAAGTGTTC 
TGCAACATGG AGACCGGCGA GAGCTGCATT TACCCCAAAC CTGCCAACAT CCCACGCAAG 
AACTGGTGGA CTACGAAGGG TGGGGATCGC AAACACATTT GGTTTGGGGA AGCCATGAAT 
GGAGGATTCC ACTTTAACTA TGGAGACGAG AATCTGTCGC CAAATACAGC CAGTATTCAG 
ATGACCTTCC TCAGACTGCT GTCCACTGAA GCCTCGCAGA ACCTTACCTA CCACTGCAGG 
AACAGCATCG CCTACATGGA CGAATCCTCT GGGAACCTGA AGAAGGCTCT GCTGCTGCGC 
GGATCCAATG ATGTGGAGAT CCGGGCGGAA GGAAACAGCC GCTTCACCTA CAGCGTTCTG 
GAGGACGGAT GCAAGAAACA CACAGGACAG TGGAGCAAGA CCGTCATTGA GTATAAATCC 
CAGAAGACGT CTCGCCTGCC CCTTGTGGAC ATTGCCCCTG TGGATATAGG AGGAGCACAT 
CAGGAGTTCG GAGTGGATGT AGGAGCCGTC TGTTTCTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Damage-induced reactive oxygen species regulate
eLife7(2018 Jan)
LeBert D,Squirrell JM,Freisinger C,Rindy J,Golenberg N,Frecentes G,Gibson A,Eliceiri KW,Huttenlocher A

Loss of DDRGK1 modulates SOX9 ubiquitination in spondyloepimetaphyseal dysplasia.
The Journal of clinical investigation127(4)1475-1484(2017 Apr)
Egunsola AT,Bae Y,Jiang MM,Liu DS,Chen-Evenson Y,Bertin T,Chen S,Lu JT,Nevarez L,Magal N,Raas-Rothschild A,Swindell EC,Cohn DH,Gibbs RA,Campeau PM,Shohat M,Lee BH

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col2a1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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