DPH6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DPH6
Entrez Gene ID	503603
Full Name	diphthamine biosynthesis 6
Synonyms	atpbd4,si:ch1073-258j7.1,wu:fc57a01,zgc:110758
General protein information	Preferred Names diphthamine biosynthesis 6 Names diphthine--ammonia ligase ATP binding domain 4 ATP-binding domain-containing protein 4 DPH6 homolog diphthamide synthase diphthamide synthetase protein DPH6 homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017351632.2	XP_017207121.2	diphthine--ammonia ligase isoform X1
NM_001013290.2	NP_001013308.2	diphthine--ammonia ligase
XM_017351632.1	XP_017207121.1	diphthine--ammonia ligase isoform X1

Pathways from BioSystems

Homologies

	ATPBD4	XP_001089692.2
Drosophila melanogaster (fruit fly)	CG1578	NP_572749.2
Kluyveromyces lactis	KLLA0D16984g	XP_453811.1
Eremothecium gossypii	AGOS_AGL164W	NP_986503.1
Schizosaccharomyces pombe (fission yeast)	mug71	NP_595310.1
Magnaporthe oryzae (rice blast fungus)	MGG_00500	XP_003718561.1
Pan troglodytes (chimpanzee)	ATPBD4	XP_001135595.2
Canis lupus familiaris (dog)	DPH6	XP_005638265.1
Mus musculus (house mouse)	Dph6	NP_079951.1
Rattus norvegicus (Norway rat)	Dph6	NP_001014203.1
Saccharomyces cerevisiae (baker's yeast)	DPH6	NP_013244.1
Neurospora crassa	NCU02670	XP_965443.2
Xenopus tropicalis (tropical clawed frog)	dph6	XP_002932882.1
Homo sapiens (human)	DPH6	NP_542381.1
Bos taurus (cattle)	DPH6	NP_001070484.1
Danio rerio (zebrafish)	dph6	NP_001013308.2
Caenorhabditis elegans (roundworm)	E01A2.5	NP_491303.3
Gallus gallus (chicken)	ATPBD4	NP_001186639.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001943	XP_321119.5

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
Kotani T, Kawakami K
Developmental biology316(2)383-96(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DPH6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DPH6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03205	NM_001013290.2 Latest version!	Danio rerio diphthamine biosynthesis 6 (dph6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa149539	XM_017351632.2 Latest version!	Danio rerio diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa55576	XM_017351632.1 Latest version!	Danio rerio diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DPH6 ( NM_001013290.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001013308.2 Danio rerio diphthamine biosynthesis 6 (dph6), mRNA.

Price & Availability↑

CloneID	ODa03205
Clone ID Related Accession (Same CDS sequence)	NM_001013290.2
Accession Version	NM_001013290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	diphthine--ammonia ligase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133118.1. On Feb 11, 2007 this sequence version replaced NM_001013290.1. ##Evidence-Data-START## Transcript exon combination :: BC133118.1, EH491372.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGGGTGG TCGGGCTCAT CAGTGGCGGC AAGGACAGCT GCTTTAACAT GCTGCAGTGT 
GTGTCTGCCG GACACTCTAT AGTCGCTCTG GCAAACCTCA GACCTGCAGA CCACGCGGCT 
TCAGATGAGC TGGACAGCTA CATGTACCAG ACTGTGGGTC ATCAGGCTGT GGATCTGATA 
GCAGAGGCCA TGGGTTTGCC GCTCTACAGG CGCACTATTG AGGGCTCGAG TGTGCATATC 
GACAGAGAGT ACAGCCCCAC TGATGGAGAC GAGGTGGAGG ATCTCTATCA GCTGCTCAAA 
CACGTCAAGG AGGAGATGCA TGTGGACGGC GTGTCTGTCG GAGCCATTCT GTCTGATTAT 
CAGAGAGTAC GAGTGGAAAA TGTGTGTGCA AGGCTGCAGC TTCAGCCGCT GGCCTACCTG 
TGGCGTCGAG ACCAGGCCGC CCTGCTCTCA GAGATGATAT CCAGCGGACT CCATGCCATC 
CTCATCAAAG TGGCAGCATT CGGTCTGCAT CCAGACAAAC ATCTAGGAAA ATCGCTGGCG 
GAGATGGAGC TGTATCTGCA CGAGCTGTCA GAGAAGTATG GCGTTCACAT CTGTGGTGAA 
GGTGGAGAAT ACGAGACCTT CACCCTCGAC TGTCCGCTCT TCAAAAAGAA GATCATCATA 
GACGCCACAG AGACCGTCAT CCACTCAGAT GATGCCTTCG CTCCTGTAGG TTTTTTGCGC 
TTCACCAAAA TGCACACTGA GGACAAGACA GAGGTGCGCA CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013308.2
CDS	95..862
Translation	MRVVGLISGGKDSCFNMLQCVSAGHSIVALANLRPADHAASDELDSYMYQTVGHQAVDLIAEAMGLPLYRRTIEGSSVHIDREYSPTDGDEVEDLYQLLKHVKEEMHVDGVSVGAILSDYQRVRVENVCARLQLQPLAYLWRRDQAALLSEMISSGLHAILIKVAAFGLHPDKHLGKSLAEMELYLHELSEKYGVHICGEGGEYETFTLDCPLFKKKIIIDATETVIHSDDAFAPVGFLRFTKMHTEDKTEVRTL

Target ORF information:

RefSeq Version	NM_001013290.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diphthamine biosynthesis 6 (dph6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013290.2

ORF Insert Sequence:

ATGAGGGTGG TCGGGCTCAT CAGTGGCGGC AAGGACAGCT GCTTTAACAT GCTGCAGTGT 
GTGTCTGCCG GACACTCTAT AGTCGCTCTG GCAAACCTCA GACCTGCAGA CCACGCGGCT 
TCAGATGAGC TGGACAGCTA CATGTACCAG ACTGTGGGTC ATCAGGCTGT GGATCTGATA 
GCAGAGGCCA TGGGTTTGCC GCTCTACAGG CGCACTATTG AGGGCTCGAG TGTGCATATC 
GACAGAGAGT ACAGCCCCAC TGATGGAGAC GAGGTGGAGG ATCTCTATCA GCTGCTCAAA 
CACGTCAAGG AGGAGATGCA TGTGGACGGC GTGTCTGTCG GAGCCATTCT GTCTGATTAT 
CAGAGAGTAC GAGTGGAAAA TGTGTGTGCA AGGCTGCAGC TTCAGCCGCT GGCCTACCTG 
TGGCGTCGAG ACCAGGCCGC CCTGCTCTCA GAGATGATAT CCAGCGGACT CCATGCCATC 
CTCATCAAAG TGGCAGCATT CGGTCTGCAT CCAGACAAAC ATCTAGGAAA ATCGCTGGCG 
GAGATGGAGC TGTATCTGCA CGAGCTGTCA GAGAAGTATG GCGTTCACAT CTGTGGTGAA 
GGTGGAGAAT ACGAGACCTT CACCCTCGAC TGTCCGCTCT TCAAAAAGAA GATCATCATA 
GACGCCACAG AGACCGTCAT CCACTCAGAT GATGCCTTCG CTCCTGTAGG TTTTTTGCGC 
TTCACCAAAA TGCACACTGA GGACAAGACA GAGGTGCGCA CACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH6 ( XM_017351632.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207121.2 Danio rerio diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149539
Clone ID Related Accession (Same CDS sequence)	XM_017351632.2
Accession Version	XM_017351632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diphthine--ammonia ligase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) and transcript sequence (GDQH01007575.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 240 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGGGTGG TCGGGCTCAT CAGTGGCGGC AAGGACAGCT GCTTTAACAT GCTGCAGTGT 
GTGTCTGCCG GACACTCTAT AGTCGCTCTG GCAAACCTCA GACCTGCAGA CCACGCGGCT 
TCAGATGAGC TGGACAGCTA CATGTACCAG ACTGTGGGTC ATCAGGCTGT GGATCTGATA 
GCAGAGGCCA TGGGTTTGCC GCTCTACAGG CGCACTATTG AGGGCTCGAG TGTGCATATC 
GACAGAGAGT ACAGCCCCAC TGATGGAGAC GAGGTGGAGG ATCTCTATCA GCTGCTCAAA 
CACGTCAAGG AGGAGATGCA TGTGGACGGC GTGTCTGTCG GAGCCATTCT GTCTGATTAT 
CAGAGAGTAC GAGTGGAAAA TGTGTGTGCA AGGCTGCAGC TTCAGCCGCT GGCCTACCTG 
TGGCGTCGAG ACCAGGCCGC CCTGCTCTCA GAGATGATAT CCAGCGGACT CCATGCCATC 
CTCATCAAAG TGGCAGCATT CGGTCTGCAT CCAGACAAAC ATCTAGGAAA ATCGCTGGCG 
GAGATGGAGC TGTATCTGCA CGAGCTGTCA GAGAAGTATG GCGTTCACAT CTGTGGTGAA 
GGTGGAGAAT ACGAGACCTT CACCCTCGAC TGTCCGCTCT TCAAAAAGAA GATCATCATA 
GACGCCACAG AGACCGTCAT CCACTCAGAT GATGCCTTCG CTCCTGTAGG TTTTTTGCGC 
TTCACCAAAA TGCACACTGA GGACAAGACA GAGGGCTCCG GAGGGCCACC ACCGCCGTCT 
CTCTCTGCAT GTCCTTGCCA GAGCGCCATT GACAGGATGA CAGAAGAGCT GGAATATGCT 
GATAAAACTG CCGACGTCCA GAGAGAATGT CCATCACACA CACAGAGCAC ATGGCAACTG 
GACGAGGGTT GTGAGGTTTC ACACTCGTCC AGCAGTTCTG GTTTCCAGTG GATCAGTGGA 
TTATCAGCGC TTCCGTCTGA ACACCCGGAC ATCCAGAGCC AAGCACAGCA CGTCTTCACA 
CTCTTGCAAA GCCGTCTGCA GGAGATGGGC TCTGCGCTCA GACATGTTCT TCTGGTTCAT 
CTGTACGTCA GCAGCATGCA GGACTTCGGG CTGATCAACA GCATCTACAG CAGACTCTTC 
ACACACAACC CGCCCGCCAG GGTGTGTGTG CAGGCGTCTC TGCCAGTCGG TCAGCAGCTG 
CAGATGGATG TTCTTCTTCA GGATCAGACG AAGGCTTCTC CATCATCGTC TTCATCAGTG 
TGTGAGGAGG AGTGTTTCCC CCAGAGAGAG ACTCTTCATG TGCAGAGTGT GTCCCACTGG 
GCCCCGGCCA ACATCGGCCC CTACAGTCAG GCCACACAGG TTGGCGGTGC AGTGTTCTGT 
GCGGGTCAGA TCCCTCTAGT CCCGTGCCGT ATGGTTCTGC TGGACGGTCC TCCACTGCTC 
CAGGCTCAGC TCTGCTTCAG TCACACAGAG AAGGTTCTGG AGGCCGTGAG CTCTGGACTG 
ACGCTGAAGA ACGTTCTGCA GGCGCACATC TACATCACAG ACGTCAAATA CAGTTCAGCC 
GTCAGCGCAG CCTGGAGAAC ACGACTGCAG GACGCACAGC TCCATCTGCT GGTGGTGTGT 
GCTCTGCCCA GAGGAGCGCT GCTGGAGCTG CATGTGTCTG CGGTGCAGGA CTGTCCCTCA 
GAGAGAGTGT GTGTGTGTCG GAGCTCCAGC GGCCTTGTGG AGTCCCGGCT GCTGCGCTCC 
GGCTGCGCTC GATACGCCTC TCTGTCTGTG AGTGTGTGTG AGAGCGCAGA GAGTGCGTGT 
GTGAGCGCCG AGAGCCTGCT GCACACACTG ATGTCCTGCT ACAGCTCTGC GCTTCAGGAG 
AGTCCCGGGC TGACTGCGCT GTGTGCCAGA GTCTTCTACA GGAGACACCA CAACACCAGC 
ACACACATCG CCACAGGTCT GCAGCAGCGC TTCACTGGTG CTCGGGATCC GTGTGCTCCT 
GCGGTGGTTC TGGTTCCTGT TGAGGATCTT GCAGAGGGTC AGCTGCTGCT GGTGTCCTGC 
TGGCTCAGTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207121.2
CDS	129..2183
Translation	MRVVGLISGGKDSCFNMLQCVSAGHSIVALANLRPADHAASDELDSYMYQTVGHQAVDLIAEAMGLPLYRRTIEGSSVHIDREYSPTDGDEVEDLYQLLKHVKEEMHVDGVSVGAILSDYQRVRVENVCARLQLQPLAYLWRRDQAALLSEMISSGLHAILIKVAAFGLHPDKHLGKSLAEMELYLHELSEKYGVHICGEGGEYETFTLDCPLFKKKIIIDATETVIHSDDAFAPVGFLRFTKMHTEDKTEGSGGPPPPSLSACPCQSAIDRMTEELEYADKTADVQRECPSHTQSTWQLDEGCEVSHSSSSSGFQWISGLSALPSEHPDIQSQAQHVFTLLQSRLQEMGSALRHVLLVHLYVSSMQDFGLINSIYSRLFTHNPPARVCVQASLPVGQQLQMDVLLQDQTKASPSSSSSVCEEECFPQRETLHVQSVSHWAPANIGPYSQATQVGGAVFCAGQIPLVPCRMVLLDGPPLLQAQLCFSHTEKVLEAVSSGLTLKNVLQAHIYITDVKYSSAVSAAWRTRLQDAQLHLLVVCALPRGALLELHVSAVQDCPSERVCVCRSSSGLVESRLLRSGCARYASLSVSVCESAESACVSAESLLHTLMSCYSSALQESPGLTALCARVFYRRHHNTSTHIATGLQQRFTGARDPCAPAVVLVPVEDLAEGQLLLVSCWLSV

Target ORF information:

RefSeq Version	XM_017351632.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351632.2

ORF Insert Sequence:

ATGAGGGTGG TCGGGCTCAT CAGTGGCGGC AAGGACAGCT GCTTTAACAT GCTGCAGTGT 
GTGTCTGCCG GACACTCTAT AGTCGCTCTG GCAAACCTCA GACCTGCAGA CCACGCGGCT 
TCAGATGAGC TGGACAGCTA CATGTACCAG ACTGTGGGTC ATCAGGCTGT GGATCTGATA 
GCAGAGGCCA TGGGTTTGCC GCTCTACAGG CGCACTATTG AGGGCTCGAG TGTGCATATC 
GACAGAGAGT ACAGCCCCAC TGATGGAGAC GAGGTGGAGG ATCTCTATCA GCTGCTCAAA 
CACGTCAAGG AGGAGATGCA TGTGGACGGC GTGTCTGTCG GAGCCATTCT GTCTGATTAT 
CAGAGAGTAC GAGTGGAAAA TGTGTGTGCA AGGCTGCAGC TTCAGCCGCT GGCCTACCTG 
TGGCGTCGAG ACCAGGCCGC CCTGCTCTCA GAGATGATAT CCAGCGGACT CCATGCCATC 
CTCATCAAAG TGGCAGCATT CGGTCTGCAT CCAGACAAAC ATCTAGGAAA ATCGCTGGCG 
GAGATGGAGC TGTATCTGCA CGAGCTGTCA GAGAAGTATG GCGTTCACAT CTGTGGTGAA 
GGTGGAGAAT ACGAGACCTT CACCCTCGAC TGTCCGCTCT TCAAAAAGAA GATCATCATA 
GACGCCACAG AGACCGTCAT CCACTCAGAT GATGCCTTCG CTCCTGTAGG TTTTTTGCGC 
TTCACCAAAA TGCACACTGA GGACAAGACA GAGGGCTCCG GAGGGCCACC ACCGCCGTCT 
CTCTCTGCAT GTCCTTGCCA GAGCGCCATT GACAGGATGA CAGAAGAGCT GGAATATGCT 
GATAAAACTG CCGACGTCCA GAGAGAATGT CCATCACACA CACAGAGCAC ATGGCAACTG 
GACGAGGGTT GTGAGGTTTC ACACTCGTCC AGCAGTTCTG GTTTCCAGTG GATCAGTGGA 
TTATCAGCGC TTCCGTCTGA ACACCCGGAC ATCCAGAGCC AAGCACAGCA CGTCTTCACA 
CTCTTGCAAA GCCGTCTGCA GGAGATGGGC TCTGCGCTCA GACATGTTCT TCTGGTTCAT 
CTGTACGTCA GCAGCATGCA GGACTTCGGG CTGATCAACA GCATCTACAG CAGACTCTTC 
ACACACAACC CGCCCGCCAG GGTGTGTGTG CAGGCGTCTC TGCCAGTCGG TCAGCAGCTG 
CAGATGGATG TTCTTCTTCA GGATCAGACG AAGGCTTCTC CATCATCGTC TTCATCAGTG 
TGTGAGGAGG AGTGTTTCCC CCAGAGAGAG ACTCTTCATG TGCAGAGTGT GTCCCACTGG 
GCCCCGGCCA ACATCGGCCC CTACAGTCAG GCCACACAGG TTGGCGGTGC AGTGTTCTGT 
GCGGGTCAGA TCCCTCTAGT CCCGTGCCGT ATGGTTCTGC TGGACGGTCC TCCACTGCTC 
CAGGCTCAGC TCTGCTTCAG TCACACAGAG AAGGTTCTGG AGGCCGTGAG CTCTGGACTG 
ACGCTGAAGA ACGTTCTGCA GGCGCACATC TACATCACAG ACGTCAAATA CAGTTCAGCC 
GTCAGCGCAG CCTGGAGAAC ACGACTGCAG GACGCACAGC TCCATCTGCT GGTGGTGTGT 
GCTCTGCCCA GAGGAGCGCT GCTGGAGCTG CATGTGTCTG CGGTGCAGGA CTGTCCCTCA 
GAGAGAGTGT GTGTGTGTCG GAGCTCCAGC GGCCTTGTGG AGTCCCGGCT GCTGCGCTCC 
GGCTGCGCTC GATACGCCTC TCTGTCTGTG AGTGTGTGTG AGAGCGCAGA GAGTGCGTGT 
GTGAGCGCCG AGAGCCTGCT GCACACACTG ATGTCCTGCT ACAGCTCTGC GCTTCAGGAG 
AGTCCCGGGC TGACTGCGCT GTGTGCCAGA GTCTTCTACA GGAGACACCA CAACACCAGC 
ACACACATCG CCACAGGTCT GCAGCAGCGC TTCACTGGTG CTCGGGATCC GTGTGCTCCT 
GCGGTGGTTC TGGTTCCTGT TGAGGATCTT GCAGAGGGTC AGCTGCTGCT GGTGTCCTGC 
TGGCTCAGTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DPH6 ( XM_017351632.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207121.1 Danio rerio diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa55576
Clone ID Related Accession (Same CDS sequence)	XM_017351632.1
Accession Version	XM_017351632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diphthine--ammonia ligase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) and transcript sequence (GDQH01007575.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 240 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGGGTGG TCGGGCTCAT CAGTGGCGGC AAGGACAGCT GCTTTAACAT GCTGCAGTGT 
GTGTCTGCCG GACACTCTAT AGTCGCTCTG GCAAACCTCA GACCTGCAGA CCACGCGGCT 
TCAGATGAGC TGGACAGCTA CATGTACCAG ACTGTGGGTC ATCAGGCTGT GGATCTGATA 
GCAGAGGCCA TGGGTTTGCC GCTCTACAGG CGCACTATTG AGGGCTCGAG TGTGCATATC 
GACAGAGAGT ACAGCCCCAC TGATGGAGAC GAGGTGGAGG ATCTCTATCA GCTGCTCAAA 
CACGTCAAGG AGGAGATGCA TGTGGACGGC GTGTCTGTCG GAGCCATTCT GTCTGATTAT 
CAGAGAGTAC GAGTGGAAAA TGTGTGTGCA AGGCTGCAGC TTCAGCCGCT GGCCTACCTG 
TGGCGTCGAG ACCAGGCCGC CCTGCTCTCA GAGATGATAT CCAGCGGACT CCATGCCATC 
CTCATCAAAG TGGCAGCATT CGGTCTGCAT CCAGACAAAC ATCTAGGAAA ATCGCTGGCG 
GAGATGGAGC TGTATCTGCA CGAGCTGTCA GAGAAGTATG GCGTTCACAT CTGTGGTGAA 
GGTGGAGAAT ACGAGACCTT CACCCTCGAC TGTCCGCTCT TCAAAAAGAA GATCATCATA 
GACGCCACAG AGACCGTCAT CCACTCAGAT GATGCCTTCG CTCCTGTAGG TTTTTTGCGC 
TTCACCAAAA TGCACACTGA GGACAAGACA GAGGGCTCCG GAGGGCCACC ACCGCCGTCT 
CTCTCTGCAT GTCCTTGCCA GAGCGCCATT GACAGGATGA CAGAAGAGCT GGAATATGCT 
GATAAAACTG CCGACGTCCA GAGAGAATGT CCATCACACA CACAGAGCAC ATGGCAACTG 
GACGAGGGTT GTGAGGTTTC ACACTCGTCC AGCAGTTCTG GTTTCCAGTG GATCAGTGGA 
TTATCAGCGC TTCCGTCTGA ACACCCGGAC ATCCAGAGCC AAGCACAGCA CGTCTTCACA 
CTCTTGCAAA GCCGTCTGCA GGAGATGGGC TCTGCGCTCA GACATGTTCT TCTGGTTCAT 
CTGTACGTCA GCAGCATGCA GGACTTCGGG CTGATCAACA GCATCTACAG CAGACTCTTC 
ACACACAACC CGCCCGCCAG GGTGTGTGTG CAGGCGTCTC TGCCAGTCGG TCAGCAGCTG 
CAGATGGATG TTCTTCTTCA GGATCAGACG AAGGCTTCTC CATCATCGTC TTCATCAGTG 
TGTGAGGAGG AGTGTTTCCC CCAGAGAGAG ACTCTTCATG TGCAGAGTGT GTCCCACTGG 
GCCCCGGCCA ACATCGGCCC CTACAGTCAG GCCACACAGG TTGGCGGTGC AGTGTTCTGT 
GCGGGTCAGA TCCCTCTAGT CCCGTGCCGT ATGGTTCTGC TGGACGGTCC TCCACTGCTC 
CAGGCTCAGC TCTGCTTCAG TCACACAGAG AAGGTTCTGG AGGCCGTGAG CTCTGGACTG 
ACGCTGAAGA ACGTTCTGCA GGCGCACATC TACATCACAG ACGTCAAATA CAGTTCAGCC 
GTCAGCGCAG CCTGGAGAAC ACGACTGCAG GACGCACAGC TCCATCTGCT GGTGGTGTGT 
GCTCTGCCCA GAGGAGCGCT GCTGGAGCTG CATGTGTCTG CGGTGCAGGA CTGTCCCTCA 
GAGAGAGTGT GTGTGTGTGG GAGCTCCAGC GGCCTTGTGG AGTCCCGGCT GCTGCGCTCC 
GGCTGCGCTC GATACGCCTC TCTGTCTCTG AGTGTGTGTG AGAGCGCAGA GAGTGTGTGT 
GTGAGCGCCG AGAGCCTGCT GCACACACTG ATGTCCTGCT ACAGCTCTGC GCTTCAGGAG 
AGTCCCGGGC TGACTGCGCT GTGTGCCAGA GTCTTCTACA GGAGACACCA CAACACCAGC 
ACACACATCG CCACAGGTCT GCAGCAGCGC TTCACTGGTG CTCGGGATCC GTGTGCTCCT 
GCGGTGGTTC TGGTTCCTGT TGAGGATCTT GCAGAGGGTC AGCTGCTGCT GGTGTCCTGC 
TGGCTCAGTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207121.1
CDS	179..2233
Translation	MRVVGLISGGKDSCFNMLQCVSAGHSIVALANLRPADHAASDELDSYMYQTVGHQAVDLIAEAMGLPLYRRTIEGSSVHIDREYSPTDGDEVEDLYQLLKHVKEEMHVDGVSVGAILSDYQRVRVENVCARLQLQPLAYLWRRDQAALLSEMISSGLHAILIKVAAFGLHPDKHLGKSLAEMELYLHELSEKYGVHICGEGGEYETFTLDCPLFKKKIIIDATETVIHSDDAFAPVGFLRFTKMHTEDKTEGSGGPPPPSLSACPCQSAIDRMTEELEYADKTADVQRECPSHTQSTWQLDEGCEVSHSSSSSGFQWISGLSALPSEHPDIQSQAQHVFTLLQSRLQEMGSALRHVLLVHLYVSSMQDFGLINSIYSRLFTHNPPARVCVQASLPVGQQLQMDVLLQDQTKASPSSSSSVCEEECFPQRETLHVQSVSHWAPANIGPYSQATQVGGAVFCAGQIPLVPCRMVLLDGPPLLQAQLCFSHTEKVLEAVSSGLTLKNVLQAHIYITDVKYSSAVSAAWRTRLQDAQLHLLVVCALPRGALLELHVSAVQDCPSERVCVCGSSSGLVESRLLRSGCARYASLSLSVCESAESVCVSAESLLHTLMSCYSSALQESPGLTALCARVFYRRHHNTSTHIATGLQQRFTGARDPCAPAVVLVPVEDLAEGQLLLVSCWLSV

Target ORF information:

RefSeq Version	XM_017351632.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diphthamine biosynthesis 6 (dph6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351632.1

ORF Insert Sequence:

ATGAGGGTGG TCGGGCTCAT CAGTGGCGGC AAGGACAGCT GCTTTAACAT GCTGCAGTGT 
GTGTCTGCCG GACACTCTAT AGTCGCTCTG GCAAACCTCA GACCTGCAGA CCACGCGGCT 
TCAGATGAGC TGGACAGCTA CATGTACCAG ACTGTGGGTC ATCAGGCTGT GGATCTGATA 
GCAGAGGCCA TGGGTTTGCC GCTCTACAGG CGCACTATTG AGGGCTCGAG TGTGCATATC 
GACAGAGAGT ACAGCCCCAC TGATGGAGAC GAGGTGGAGG ATCTCTATCA GCTGCTCAAA 
CACGTCAAGG AGGAGATGCA TGTGGACGGC GTGTCTGTCG GAGCCATTCT GTCTGATTAT 
CAGAGAGTAC GAGTGGAAAA TGTGTGTGCA AGGCTGCAGC TTCAGCCGCT GGCCTACCTG 
TGGCGTCGAG ACCAGGCCGC CCTGCTCTCA GAGATGATAT CCAGCGGACT CCATGCCATC 
CTCATCAAAG TGGCAGCATT CGGTCTGCAT CCAGACAAAC ATCTAGGAAA ATCGCTGGCG 
GAGATGGAGC TGTATCTGCA CGAGCTGTCA GAGAAGTATG GCGTTCACAT CTGTGGTGAA 
GGTGGAGAAT ACGAGACCTT CACCCTCGAC TGTCCGCTCT TCAAAAAGAA GATCATCATA 
GACGCCACAG AGACCGTCAT CCACTCAGAT GATGCCTTCG CTCCTGTAGG TTTTTTGCGC 
TTCACCAAAA TGCACACTGA GGACAAGACA GAGGGCTCCG GAGGGCCACC ACCGCCGTCT 
CTCTCTGCAT GTCCTTGCCA GAGCGCCATT GACAGGATGA CAGAAGAGCT GGAATATGCT 
GATAAAACTG CCGACGTCCA GAGAGAATGT CCATCACACA CACAGAGCAC ATGGCAACTG 
GACGAGGGTT GTGAGGTTTC ACACTCGTCC AGCAGTTCTG GTTTCCAGTG GATCAGTGGA 
TTATCAGCGC TTCCGTCTGA ACACCCGGAC ATCCAGAGCC AAGCACAGCA CGTCTTCACA 
CTCTTGCAAA GCCGTCTGCA GGAGATGGGC TCTGCGCTCA GACATGTTCT TCTGGTTCAT 
CTGTACGTCA GCAGCATGCA GGACTTCGGG CTGATCAACA GCATCTACAG CAGACTCTTC 
ACACACAACC CGCCCGCCAG GGTGTGTGTG CAGGCGTCTC TGCCAGTCGG TCAGCAGCTG 
CAGATGGATG TTCTTCTTCA GGATCAGACG AAGGCTTCTC CATCATCGTC TTCATCAGTG 
TGTGAGGAGG AGTGTTTCCC CCAGAGAGAG ACTCTTCATG TGCAGAGTGT GTCCCACTGG 
GCCCCGGCCA ACATCGGCCC CTACAGTCAG GCCACACAGG TTGGCGGTGC AGTGTTCTGT 
GCGGGTCAGA TCCCTCTAGT CCCGTGCCGT ATGGTTCTGC TGGACGGTCC TCCACTGCTC 
CAGGCTCAGC TCTGCTTCAG TCACACAGAG AAGGTTCTGG AGGCCGTGAG CTCTGGACTG 
ACGCTGAAGA ACGTTCTGCA GGCGCACATC TACATCACAG ACGTCAAATA CAGTTCAGCC 
GTCAGCGCAG CCTGGAGAAC ACGACTGCAG GACGCACAGC TCCATCTGCT GGTGGTGTGT 
GCTCTGCCCA GAGGAGCGCT GCTGGAGCTG CATGTGTCTG CGGTGCAGGA CTGTCCCTCA 
GAGAGAGTGT GTGTGTGTGG GAGCTCCAGC GGCCTTGTGG AGTCCCGGCT GCTGCGCTCC 
GGCTGCGCTC GATACGCCTC TCTGTCTCTG AGTGTGTGTG AGAGCGCAGA GAGTGTGTGT 
GTGAGCGCCG AGAGCCTGCT GCACACACTG ATGTCCTGCT ACAGCTCTGC GCTTCAGGAG 
AGTCCCGGGC TGACTGCGCT GTGTGCCAGA GTCTTCTACA GGAGACACCA CAACACCAGC 
ACACACATCG CCACAGGTCT GCAGCAGCGC TTCACTGGTG CTCGGGATCC GTGTGCTCCT 
GCGGTGGTTC TGGTTCCTGT TGAGGATCTT GCAGAGGGTC AGCTGCTGCT GGTGTCCTGC 
TGGCTCAGTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance.
Developmental biology316(2)383-96(2008 Apr)
Kotani T,Kawakami K

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DPH6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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