ext1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ext1b
Entrez Gene ID	497280
Full Name	exostosin glycosyltransferase 1b
General protein information	Preferred Names exostosin glycosyltransferase 1b Names exostosin-1b exostoses (multiple) 1b glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase 1b multiple exostoses protein 1 homolog b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012369.2	NP_001012369.2	exostosin-1b

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ext1b	NP_001012369.2
Xenopus tropicalis (tropical clawed frog)	ext1	NP_001006730.1
Homo sapiens (human)	EXT1	NP_000118.2
	EXT1	XP_001096808.1
Gallus gallus (chicken)	LOC100858205	XP_003640902.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010388	XP_311558.3
Canis lupus familiaris (dog)	EXT1	XP_539145.2
Bos taurus (cattle)	EXT1	NP_001091564.1
Mus musculus (house mouse)	Ext1	NP_034292.2
Rattus norvegicus (Norway rat)	Ext1	NP_001124012.1
Caenorhabditis elegans (roundworm)	rib-1	NP_502180.1
Pan troglodytes (chimpanzee)	EXT1	XP_001141496.1
Drosophila melanogaster (fruit fly)	ttv	NP_477231.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals EXT-1 tumor suppressor as a Notch1 and FBWX7 common interactor.
Daakour S, Hajingabo LJ, Kerselidou D, Devresse A, Kettmann R, Simonis N, Dequiedt F, Twizere JC
BMC cancer16335(2016 05)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Briolat V, Jouneau L, Carvalho R, Palha N, Langevin C, Herbomel P, Schwartz O, Spaink HP, Levraud JP, Boudinot P
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)

Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling.
Siekmann AF, Brand M
Developmental dynamics : an official publication of the American Association of Anatomists232(2)498-505(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the three zebrafish ext1 genes encode proteoglycan modifying enzymes and have distinct relationships to somitic Sonic hedgehog signaling
Title: Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling.

The following ext1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ext1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03233	NM_001012369.2 Latest version!	Danio rerio exostosin glycosyltransferase 1b (ext1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ext1b ( NM_001012369.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001012369.2 Danio rerio exostosin glycosyltransferase 1b (ext1b), mRNA.

Price & Availability↑

CloneID	ODa03233
Clone ID Related Accession (Same CDS sequence)	NM_001012369.2
Accession Version	NM_001012369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Danio rerio(zebrafish)
Product	exostosin-1b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125900.1. On Nov 8, 2006 this sequence version replaced NM_001012369.1. ##Evidence-Data-START## Transcript exon combination :: BC125900.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCAGGCCA AAAAACGATA TCTGATCTCG CTCTTGACTG GCGCCTTTCT AGTTCTGCTC 
ATTTACTTGG GAGGAGGAGG GGTCCCGGGT CCCGCGGCCC CGGGCTCCAG GAGCCGGACA 
CATGGATACA ACCGGCCCGA TCAGCCGTGG CCACACTTCT CGGACCCCTT ACAACACTTC 
AGCCCGTGGG ACCACTCGGA CACGGAGGAC TATAACGTGC ACATATCGCC CAGGCAGAAG 
AGAGATGTGA ACTCCAGCGT GTATAAGGGT AAGCGGTGCC GCATGCAATC CTGCTTTGAT 
TTCTCGCTCT GCCAACGGAA CGGCTTCAAA GTCTACGTGT ATCCGCAGCA GAAGGGGGAG 
AAGATATCGG AAAGTTACCA GAACATCTTA TCCACCATCG AGGGCTCTAG ATTTTACACC 
TCTGACCCTG GTCAGGCGTG CGTGTTTGTG CTGAGCTTGG ACACTCTCGA CCGAGACCAG 
TTGTCTCCGC AGTATGTGCA CAATCTCAAA ACTAAAGTGC AGAGCTTGGC TCTGTGGAAC 
AACGGTAGGA ATCACCTCAT ATTTAATTTG TATTCTGGCA CATGGCCTGA CTACACGGAA 
GATTTGGGCT TTGACATTGG TCAGGCCATG CTGGCCAAAG CTAGCATCAG CACCGAGAAC 
TTCAGACCCA ACTTTGACAT CTCCATCCCC TTGTTCTCCA AGGAGCACCC GAGGACCGGC 
GGGGACAGGG GCTACTTGAA ATACAACACC ATTCCACCTT TTAGAAAGTA TATGTTGGTA 
TTTAAGGGCA AGCGCTATTT GACAGGCATC GGCTCGGACA CGAGGAATGC TTTATATCAC 
GTTCATAACG CGGAGGATGT AGTGCTGCTC ACCACCTGCA AACATGGCAA AGACTGGCAG 
AAACACAAGG ACGCGCGCTG TGACAAGGAC AACGCAGAGT ATGACAAGTA CGACTACCGT 
GAAATGCTGC ACAACTCTAC CTTCTGTCTG GTTCCTCGAG GACGACGCCT GGGATCCTTC 
CGCTTCCTGG AGGCTCTTCA GGCGGCCTGT GTGCCGGTGA TGCTGAGTAA CGGTTGGGAG 
CTCCCCTTCT CGGAGGTCAT CGACTGGAAC ACTGCTGCTG TGATCGGGGA CGAGAGGCTC 
CTGCTGCAGA TCCCGTCCAC TGTTCGCTCC ATCCATCAGG ACAAGATCCT GGCTCTCCGA 
CAGCAGACGC AGTTCCTCTG GGAGGCCTAT TTCTCTTCAG TGGAGAAGAT CGTGCTCACC 
ACTCTAGAAA TCATCCAGGA CCGGGTGCTC CAGCAGAGCT CCAGAAGCAG TGTGATGTGG 
AACAGTCACC CCGGTGGACT CTTCTCTCTG CCGCAGTACT CCTCATATCT GGGAGACTTT 
CCTTTCTTCT ACGCCAAACT CGGCATCAAG CCGAGTCCCA AGTTCACTGC AGTTATCCAT 
GCAGTGACGC CGCTGGTTTC TCAATCTCAG CCCATTCTCA AGCTCATCGT CTCAGTGGCC 
AGATCACAGT ACTGCGCTCA GATTATTGTG TTGTGGAACT GTGACAAGCC TTTACCTGCC 
AAACAGCGCT GGCCGGCCAC TGCAGTGCCC ATTATAGTGA TTGAAGGAGA AAACAAGGTG 
ATGAGCAGCC GTTTTCAGCC GTATGAAAGC CTCATCAGTG ATGCGGTGTT GAGTCTGGAT 
GAAGACACTG TGTTGTCCAC CACTGAGGTG GACTTTGCCT TCACGGTGTG GCAGAGTTTC 
CCGGAGAGGA TTGTGGGATA TCCTGCCCGC AGTCATTTCT GGGACAATAA TAAGGAGCGC 
TGGGGTTACA CTTCCAAATG GACCAACGAC TATTCAATGG TTCTCACCGG AGCTGCCATT 
TACCACAGAT ATTACCACTT CCTGTACACC CACTTCCTGC CAAGCAGCCT GAAGAGTATG 
GTGGACCAGC TGGCCAACTG TGAGGATATC CTGATGAACT TCCTGGTGTC TGCGGTCACT 
AAACTGCCAC CGGTCAAAGT CACGCAGAAG AAGCAGTACA AGGAGACCAT GATGGGTCAG 
TCGTCTCGAG CCTCGCGCTG GGCCGATCCG GACCACTTCG CCCAGCGGCA GACCTGCATG 
AACAAGTTCG CCAGCTGGTT TGGCGGCATG CCCCTGGTGC ACTCCCAAAT GAGACTGGAC 
CCCGTTCTGT TCAAGGACCA GGTCTCCATC CTGCGCAAGA AATACAGGGA GATCGAGCGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012369.2
CDS	449..2674
Translation	MQAKKRYLISLLTGAFLVLLIYLGGGGVPGPAAPGSRSRTHGYNRPDQPWPHFSDPLQHFSPWDHSDTEDYNVHISPRQKRDVNSSVYKGKRCRMQSCFDFSLCQRNGFKVYVYPQQKGEKISESYQNILSTIEGSRFYTSDPGQACVFVLSLDTLDRDQLSPQYVHNLKTKVQSLALWNNGRNHLIFNLYSGTWPDYTEDLGFDIGQAMLAKASISTENFRPNFDISIPLFSKEHPRTGGDRGYLKYNTIPPFRKYMLVFKGKRYLTGIGSDTRNALYHVHNAEDVVLLTTCKHGKDWQKHKDARCDKDNAEYDKYDYREMLHNSTFCLVPRGRRLGSFRFLEALQAACVPVMLSNGWELPFSEVIDWNTAAVIGDERLLLQIPSTVRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTTLEIIQDRVLQQSSRSSVMWNSHPGGLFSLPQYSSYLGDFPFFYAKLGIKPSPKFTAVIHAVTPLVSQSQPILKLIVSVARSQYCAQIIVLWNCDKPLPAKQRWPATAVPIIVIEGENKVMSSRFQPYESLISDAVLSLDEDTVLSTTEVDFAFTVWQSFPERIVGYPARSHFWDNNKERWGYTSKWTNDYSMVLTGAAIYHRYYHFLYTHFLPSSLKSMVDQLANCEDILMNFLVSAVTKLPPVKVTQKKQYKETMMGQSSRASRWADPDHFAQRQTCMNKFASWFGGMPLVHSQMRLDPVLFKDQVSILRKKYREIERL

Target ORF information:

RefSeq Version	NM_001012369.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio exostosin glycosyltransferase 1b (ext1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012369.2

ORF Insert Sequence:

ATGCAGGCCA AAAAACGATA TCTGATCTCG CTCTTGACTG GCGCCTTTCT AGTTCTGCTC 
ATTTACTTGG GAGGAGGAGG GGTCCCGGGT CCCGCGGCCC CGGGCTCCAG GAGCCGGACA 
CATGGATACA ACCGGCCCGA TCAGCCGTGG CCACACTTCT CGGACCCCTT ACAACACTTC 
AGCCCGTGGG ACCACTCGGA CACGGAGGAC TATAACGTGC ACATATCGCC CAGGCAGAAG 
AGAGATGTGA ACTCCAGCGT GTATAAGGGT AAGCGGTGCC GCATGCAATC CTGCTTTGAT 
TTCTCGCTCT GCCAACGGAA CGGCTTCAAA GTCTACGTGT ATCCGCAGCA GAAGGGGGAG 
AAGATATCGG AAAGTTACCA GAACATCTTA TCCACCATCG AGGGCTCTAG ATTTTACACC 
TCTGACCCTG GTCAGGCGTG CGTGTTTGTG CTGAGCTTGG ACACTCTCGA CCGAGACCAG 
TTGTCTCCGC AGTATGTGCA CAATCTCAAA ACTAAAGTGC AGAGCTTGGC TCTGTGGAAC 
AACGGTAGGA ATCACCTCAT ATTTAATTTG TATTCTGGCA CATGGCCTGA CTACACGGAA 
GATTTGGGCT TTGACATTGG TCAGGCCATG CTGGCCAAAG CTAGCATCAG CACCGAGAAC 
TTCAGACCCA ACTTTGACAT CTCCATCCCC TTGTTCTCCA AGGAGCACCC GAGGACCGGC 
GGGGACAGGG GCTACTTGAA ATACAACACC ATTCCACCTT TTAGAAAGTA TATGTTGGTA 
TTTAAGGGCA AGCGCTATTT GACAGGCATC GGCTCGGACA CGAGGAATGC TTTATATCAC 
GTTCATAACG CGGAGGATGT AGTGCTGCTC ACCACCTGCA AACATGGCAA AGACTGGCAG 
AAACACAAGG ACGCGCGCTG TGACAAGGAC AACGCAGAGT ATGACAAGTA CGACTACCGT 
GAAATGCTGC ACAACTCTAC CTTCTGTCTG GTTCCTCGAG GACGACGCCT GGGATCCTTC 
CGCTTCCTGG AGGCTCTTCA GGCGGCCTGT GTGCCGGTGA TGCTGAGTAA CGGTTGGGAG 
CTCCCCTTCT CGGAGGTCAT CGACTGGAAC ACTGCTGCTG TGATCGGGGA CGAGAGGCTC 
CTGCTGCAGA TCCCGTCCAC TGTTCGCTCC ATCCATCAGG ACAAGATCCT GGCTCTCCGA 
CAGCAGACGC AGTTCCTCTG GGAGGCCTAT TTCTCTTCAG TGGAGAAGAT CGTGCTCACC 
ACTCTAGAAA TCATCCAGGA CCGGGTGCTC CAGCAGAGCT CCAGAAGCAG TGTGATGTGG 
AACAGTCACC CCGGTGGACT CTTCTCTCTG CCGCAGTACT CCTCATATCT GGGAGACTTT 
CCTTTCTTCT ACGCCAAACT CGGCATCAAG CCGAGTCCCA AGTTCACTGC AGTTATCCAT 
GCAGTGACGC CGCTGGTTTC TCAATCTCAG CCCATTCTCA AGCTCATCGT CTCAGTGGCC 
AGATCACAGT ACTGCGCTCA GATTATTGTG TTGTGGAACT GTGACAAGCC TTTACCTGCC 
AAACAGCGCT GGCCGGCCAC TGCAGTGCCC ATTATAGTGA TTGAAGGAGA AAACAAGGTG 
ATGAGCAGCC GTTTTCAGCC GTATGAAAGC CTCATCAGTG ATGCGGTGTT GAGTCTGGAT 
GAAGACACTG TGTTGTCCAC CACTGAGGTG GACTTTGCCT TCACGGTGTG GCAGAGTTTC 
CCGGAGAGGA TTGTGGGATA TCCTGCCCGC AGTCATTTCT GGGACAATAA TAAGGAGCGC 
TGGGGTTACA CTTCCAAATG GACCAACGAC TATTCAATGG TTCTCACCGG AGCTGCCATT 
TACCACAGAT ATTACCACTT CCTGTACACC CACTTCCTGC CAAGCAGCCT GAAGAGTATG 
GTGGACCAGC TGGCCAACTG TGAGGATATC CTGATGAACT TCCTGGTGTC TGCGGTCACT 
AAACTGCCAC CGGTCAAAGT CACGCAGAAG AAGCAGTACA AGGAGACCAT GATGGGTCAG 
TCGTCTCGAG CCTCGCGCTG GGCCGATCCG GACCACTTCG CCCAGCGGCA GACCTGCATG 
AACAAGTTCG CCAGCTGGTT TGGCGGCATG CCCCTGGTGC ACTCCCAAAT GAGACTGGAC 
CCCGTTCTGT TCAAGGACCA GGTCTCCATC CTGCGCAAGA AATACAGGGA GATCGAGCGG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic interactome mapping of acute lymphoblastic leukemia cancer gene products reveals EXT-1 tumor suppressor as a Notch1 and FBWX7 common interactor.
BMC cancer16335(2016 05)
Daakour S,Hajingabo LJ,Kerselidou D,Devresse A,Kettmann R,Simonis N,Dequiedt F,Twizere JC

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Contrasted innate responses to two viruses in zebrafish: insights into the ancestral repertoire of vertebrate IFN-stimulated genes.
Journal of immunology (Baltimore, Md. : 1950)192(9)4328-41(2014 May)
Briolat V,Jouneau L,Carvalho R,Palha N,Langevin C,Herbomel P,Schwartz O,Spaink HP,Levraud JP,Boudinot P

Distinct tissue-specificity of three zebrafish ext1 genes encoding proteoglycan modifying enzymes and their relationship to somitic Sonic hedgehog signaling.
Developmental dynamics : an official publication of the American Association of Anatomists232(2)498-505(2005 Feb)
Siekmann AF,Brand M

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ext1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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