prkar1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	prkar1aa
Entrez Gene ID	494533
Full Name	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a
Synonyms	im:7047729,prkar1a,zgc:92515
General protein information	Preferred Names protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a Names protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164023.2	XP_005164080.1	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a isoform X1
NM_001009989.3	NP_001009989.1	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a
XM_005164023.3	XP_005164080.1	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a isoform X1

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006448	XP_001688871.1
Danio rerio (zebrafish)	prkar1ab	NP_001017732.1
Drosophila melanogaster (fruit fly)	Pka-R1	NP_730573.2
Homo sapiens (human)	PRKAR1A	NP_997637.1
Pan troglodytes (chimpanzee)	PRKAR1A	XP_003315746.1
	LOC719021	XP_001112547.2
Canis lupus familiaris (dog)	PRKAR1A	XP_005624335.1
Bos taurus (cattle)	PRKAR1A	NP_001069826.1
Gallus gallus (chicken)	PRKAR1A	NP_001007846.1
Danio rerio (zebrafish)	prkar1aa	NP_001009989.1
Mus musculus (house mouse)	Prkar1a	NP_068680.1
Rattus norvegicus (Norway rat)	Prkar1a	NP_037313.1
Xenopus tropicalis (tropical clawed frog)	prkar1a	NP_001025530.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mys protein regulates protein kinase A activity by interacting with regulatory type Ialpha subunit during vertebrate development.
Kotani T, Iemura S, Natsume T, Kawakami K, Yamashita M
The Journal of biological chemistry285(7)5106-16(2010 Feb)

Combination of reverse and chemical genetic screens reveals angiogenesis inhibitors and targets.
Kal?n M, Wallgard E, Asker N, Nasevicius A, Athley E, Billgren E, Larson JD, Wadman SA, Norseng E, Clark KJ, He L, Karlsson-Lindahl L, H?ger AK, Weber H, Augustin H, Samuelsson T, Kemmet CK, Utesch CM, Essner JJ, Hackett PB, Hellstr?m M
Chemistry & biology16(4)432-41(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that PKA activity regulated by Mys is indispensable for negative regulation of the Hh signaling pathway in Hh-responsive cells.
Title: Mys protein regulates protein kinase A activity by interacting with regulatory type Ialpha subunit during vertebrate development.

The following prkar1aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prkar1aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08453	NM_001009989.3 Latest version!	Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa08453	XM_005164023.3 Latest version!	Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa08453	XM_005164023.2	Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prkar1aa ( NM_001009989.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001009989.1 Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), mRNA.

Price & Availability↑

CloneID	ODa08453
Clone ID Related Accession (Same CDS sequence)	XM_005164023.2 , NM_001009989.3 , XM_005164023.3
Accession Version	NM_001009989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX897685.6 and BC088390.1. On Jul 17, 2010 this sequence version replaced NM_001009989.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC088390.1, GFIL01031401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN06350657 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCCA GCAGTACGAG CAGTGAAGAA GAGAGGAGCT TGCGGGAGTG TGAGCGCTAC 
GTGCAGAAGC ACAACATCCA GCAGCTGCTG AAGGACTGCA TTGTGCAGCT GTGCACCGCC 
AGGCCTGAAA GACCCATGGC TTACCTCAGA GACTACTTCG AGAAGCTTGA GTTGGAGGAG 
GCTAAACAGA TGGTGAGCCA GCAGAAGTCC AGCTCTCGCT CAGATTCTCG GGAAGATGAG 
GTCTCTCCTC CTATGAACCC TGTGGTTAAA GGCCGACGGC GCCGTGGTGC CATCAGTGCA 
GAGGTCTACA CTGAGGAGGA CGCCACTTCT TATGTCAGAA AGGTCATTCC TAAAGACTAC 
AAAACTATGG CTGCCCTTGC TAAAGCAATT GAAAAGAATG TTCTTTTTGC TCATCTTGAC 
GACAATGAAA GAAGTGACAT ATTTGACGCC ATGTTCTCAG TCACCTACAT TGCAGGGGAG 
ACTGTCATTC AGCAAGGGGA TGAAGGAGAT AATTTTTACG TTATTGATCA AGGTGAAATG 
GATGTGTATG TGAACAATGA GTGGGTGACC AGTATTGGAG AAGGTGGCAG TTTTGGTGAA 
CTGGCTTTAA TCTACGGCAC TCCTAGAGCA GCCACTGTCA GAGCAAAGAC CAATGTCAAG 
CTGTGGGGCA TTGACAGAGA CAGCTACAGG AGAATACTCA TGGGAAGTAC TTTAAGAAAG 
AGGAAAATGT ATGAGGAATT CCTTAGCAAG GTGTCCATTT TGGAGTCTCT GGATAAATGG 
GAACGATTGA CAGTCGCTGA TGCACTTGAG ACAGTCCAGT TTGAAGATGG CCAGAAGATT 
GTGGTGCAGG GTCAACCAGG AGATGAGTTC TTCATAATTC TTGAGGGCTC GGCTGCGGTC 
TTGCAACGGC GGTCGGAGAA TGAAGAATTT GTGGAGGTTG GAAGACTCGC ACCGTCTGAC 
TACTTCGGAG AGATCGCTCT GCTCATGAAC CGTCCTCGTG CTGCCACCGT GGTTGCCCGC 
GGCCCGCTGA AGTGTGTAAA ACTCGACCGT CCTCGGTTCG AGCGTGTGCT CGGCCCCTGC 
TCAGATATCC TGAAGAGAAA CATCCAGCAG TACAACAGCT TCGTATCTCT GTCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009989.1
CDS	105..1244
Translation	MASSSTSSEEERSLRECERYVQKHNIQQLLKDCIVQLCTARPERPMAYLRDYFEKLELEEAKQMVSQQKSSSRSDSREDEVSPPMNPVVKGRRRRGAISAEVYTEEDATSYVRKVIPKDYKTMAALAKAIEKNVLFAHLDDNERSDIFDAMFSVTYIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWVTSIGEGGSFGELALIYGTPRAATVRAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALETVQFEDGQKIVVQGQPGDEFFIILEGSAAVLQRRSENEEFVEVGRLAPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	NM_001009989.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009989.3

ORF Insert Sequence:

ATGGCGTCCA GCAGTACGAG CAGTGAAGAA GAGAGGAGCT TGCGGGAGTG TGAGCGCTAC 
GTGCAGAAGC ACAACATCCA GCAGCTGCTG AAGGACTGCA TTGTGCAGCT GTGCACCGCC 
AGGCCTGAAA GACCCATGGC TTACCTCAGA GACTACTTCG AGAAGCTTGA GTTGGAGGAG 
GCTAAACAGA TGGTGAGCCA GCAGAAGTCC AGCTCTCGCT CAGATTCTCG GGAAGATGAG 
GTCTCTCCTC CTATGAACCC TGTGGTTAAA GGCCGACGGC GCCGTGGTGC CATCAGTGCA 
GAGGTCTACA CTGAGGAGGA CGCCACTTCT TATGTCAGAA AGGTCATTCC TAAAGACTAC 
AAAACTATGG CTGCCCTTGC TAAAGCAATT GAAAAGAATG TTCTTTTTGC TCATCTTGAC 
GACAATGAAA GAAGTGACAT ATTTGACGCC ATGTTCTCAG TCACCTACAT TGCAGGGGAG 
ACTGTCATTC AGCAAGGGGA TGAAGGAGAT AATTTTTACG TTATTGATCA AGGTGAAATG 
GATGTGTATG TGAACAATGA GTGGGTGACC AGTATTGGAG AAGGTGGCAG TTTTGGTGAA 
CTGGCTTTAA TCTACGGCAC TCCTAGAGCA GCCACTGTCA GAGCAAAGAC CAATGTCAAG 
CTGTGGGGCA TTGACAGAGA CAGCTACAGG AGAATACTCA TGGGAAGTAC TTTAAGAAAG 
AGGAAAATGT ATGAGGAATT CCTTAGCAAG GTGTCCATTT TGGAGTCTCT GGATAAATGG 
GAACGATTGA CAGTCGCTGA TGCACTTGAG ACAGTCCAGT TTGAAGATGG CCAGAAGATT 
GTGGTGCAGG GTCAACCAGG AGATGAGTTC TTCATAATTC TTGAGGGCTC GGCTGCGGTC 
TTGCAACGGC GGTCGGAGAA TGAAGAATTT GTGGAGGTTG GAAGACTCGC ACCGTCTGAC 
TACTTCGGAG AGATCGCTCT GCTCATGAAC CGTCCTCGTG CTGCCACCGT GGTTGCCCGC 
GGCCCGCTGA AGTGTGTAAA ACTCGACCGT CCTCGGTTCG AGCGTGTGCT CGGCCCCTGC 
TCAGATATCC TGAAGAGAAA CATCCAGCAG TACAACAGCT TCGTATCTCT GTCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkar1aa ( XM_005164023.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164080.1 Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08453
Clone ID Related Accession (Same CDS sequence)	XM_005164023.2 , NM_001009989.3 , XM_005164023.3
Accession Version	XM_005164023.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164023.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA GCAGTACGAG CAGTGAAGAA GAGAGGAGCT TGCGGGAGTG TGAGCGCTAC 
GTGCAGAAGC ACAACATCCA GCAGCTGCTG AAGGACTGCA TTGTGCAGCT GTGCACCGCC 
AGGCCTGAAA GACCCATGGC TTACCTCAGA GACTACTTCG AGAAGCTTGA GTTGGAGGAG 
GCTAAACAGA TGGTGAGCCA GCAGAAGTCC AGCTCTCGCT CAGATTCTCG GGAAGATGAG 
GTCTCTCCTC CTATGAACCC TGTGGTTAAA GGCCGACGGC GCCGTGGTGC CATCAGTGCA 
GAGGTCTACA CTGAGGAGGA CGCCACTTCT TATGTCAGAA AGGTCATTCC TAAAGACTAC 
AAAACTATGG CTGCCCTTGC TAAAGCAATT GAAAAGAATG TTCTTTTTGC TCATCTTGAC 
GACAATGAAA GAAGTGACAT ATTTGACGCC ATGTTCTCAG TCACCTACAT TGCAGGGGAG 
ACTGTCATTC AGCAAGGGGA TGAAGGAGAT AATTTTTACG TTATTGATCA AGGTGAAATG 
GATGTGTATG TGAACAATGA GTGGGTGACC AGTATTGGAG AAGGTGGCAG TTTTGGTGAA 
CTGGCTTTAA TCTACGGCAC TCCTAGAGCA GCCACTGTCA GAGCAAAGAC CAATGTCAAG 
CTGTGGGGCA TTGACAGAGA CAGCTACAGG AGAATACTCA TGGGAAGTAC TTTAAGAAAG 
AGGAAAATGT ATGAGGAATT CCTTAGCAAG GTGTCCATTT TGGAGTCTCT GGATAAATGG 
GAACGATTGA CAGTCGCTGA TGCACTTGAG ACAGTCCAGT TTGAAGATGG CCAGAAGATT 
GTGGTGCAGG GTCAACCAGG AGATGAGTTC TTCATAATTC TTGAGGGCTC GGCTGCGGTC 
TTGCAACGGC GGTCGGAGAA TGAAGAATTT GTGGAGGTTG GAAGACTCGC ACCGTCTGAC 
TACTTCGGAG AGATCGCTCT GCTCATGAAC CGTCCTCGTG CTGCCACCGT GGTTGCCCGC 
GGCCCGCTGA AGTGTGTAAA ACTCGACCGT CCTCGGTTCG AGCGTGTGCT CGGCCCCTGC 
TCAGATATCC TGAAGAGAAA CATCCAGCAG TACAACAGCT TCGTATCTCT GTCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164080.1
CDS	122..1261
Translation	MASSSTSSEEERSLRECERYVQKHNIQQLLKDCIVQLCTARPERPMAYLRDYFEKLELEEAKQMVSQQKSSSRSDSREDEVSPPMNPVVKGRRRRGAISAEVYTEEDATSYVRKVIPKDYKTMAALAKAIEKNVLFAHLDDNERSDIFDAMFSVTYIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWVTSIGEGGSFGELALIYGTPRAATVRAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALETVQFEDGQKIVVQGQPGDEFFIILEGSAAVLQRRSENEEFVEVGRLAPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	XM_005164023.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164023.2

ORF Insert Sequence:

ATGGCGTCCA GCAGTACGAG CAGTGAAGAA GAGAGGAGCT TGCGGGAGTG TGAGCGCTAC 
GTGCAGAAGC ACAACATCCA GCAGCTGCTG AAGGACTGCA TTGTGCAGCT GTGCACCGCC 
AGGCCTGAAA GACCCATGGC TTACCTCAGA GACTACTTCG AGAAGCTTGA GTTGGAGGAG 
GCTAAACAGA TGGTGAGCCA GCAGAAGTCC AGCTCTCGCT CAGATTCTCG GGAAGATGAG 
GTCTCTCCTC CTATGAACCC TGTGGTTAAA GGCCGACGGC GCCGTGGTGC CATCAGTGCA 
GAGGTCTACA CTGAGGAGGA CGCCACTTCT TATGTCAGAA AGGTCATTCC TAAAGACTAC 
AAAACTATGG CTGCCCTTGC TAAAGCAATT GAAAAGAATG TTCTTTTTGC TCATCTTGAC 
GACAATGAAA GAAGTGACAT ATTTGACGCC ATGTTCTCAG TCACCTACAT TGCAGGGGAG 
ACTGTCATTC AGCAAGGGGA TGAAGGAGAT AATTTTTACG TTATTGATCA AGGTGAAATG 
GATGTGTATG TGAACAATGA GTGGGTGACC AGTATTGGAG AAGGTGGCAG TTTTGGTGAA 
CTGGCTTTAA TCTACGGCAC TCCTAGAGCA GCCACTGTCA GAGCAAAGAC CAATGTCAAG 
CTGTGGGGCA TTGACAGAGA CAGCTACAGG AGAATACTCA TGGGAAGTAC TTTAAGAAAG 
AGGAAAATGT ATGAGGAATT CCTTAGCAAG GTGTCCATTT TGGAGTCTCT GGATAAATGG 
GAACGATTGA CAGTCGCTGA TGCACTTGAG ACAGTCCAGT TTGAAGATGG CCAGAAGATT 
GTGGTGCAGG GTCAACCAGG AGATGAGTTC TTCATAATTC TTGAGGGCTC GGCTGCGGTC 
TTGCAACGGC GGTCGGAGAA TGAAGAATTT GTGGAGGTTG GAAGACTCGC ACCGTCTGAC 
TACTTCGGAG AGATCGCTCT GCTCATGAAC CGTCCTCGTG CTGCCACCGT GGTTGCCCGC 
GGCCCGCTGA AGTGTGTAAA ACTCGACCGT CCTCGGTTCG AGCGTGTGCT CGGCCCCTGC 
TCAGATATCC TGAAGAGAAA CATCCAGCAG TACAACAGCT TCGTATCTCT GTCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prkar1aa ( XM_005164023.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164080.1 Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08453
Clone ID Related Accession (Same CDS sequence)	XM_005164023.2 , NM_001009989.3 , XM_005164023.3
Accession Version	XM_005164023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA GCAGTACGAG CAGTGAAGAA GAGAGGAGCT TGCGGGAGTG TGAGCGCTAC 
GTGCAGAAGC ACAACATCCA GCAGCTGCTG AAGGACTGCA TTGTGCAGCT GTGCACCGCC 
AGGCCTGAAA GACCCATGGC TTACCTCAGA GACTACTTCG AGAAGCTTGA GTTGGAGGAG 
GCTAAACAGA TGGTGAGCCA GCAGAAGTCC AGCTCTCGCT CAGATTCTCG GGAAGATGAG 
GTCTCTCCTC CTATGAACCC TGTGGTTAAA GGCCGACGGC GCCGTGGTGC CATCAGTGCA 
GAGGTCTACA CTGAGGAGGA CGCCACTTCT TATGTCAGAA AGGTCATTCC TAAAGACTAC 
AAAACTATGG CTGCCCTTGC TAAAGCAATT GAAAAGAATG TTCTTTTTGC TCATCTTGAC 
GACAATGAAA GAAGTGACAT ATTTGACGCC ATGTTCTCAG TCACCTACAT TGCAGGGGAG 
ACTGTCATTC AGCAAGGGGA TGAAGGAGAT AATTTTTACG TTATTGATCA AGGTGAAATG 
GATGTGTATG TGAACAATGA GTGGGTGACC AGTATTGGAG AAGGTGGCAG TTTTGGTGAA 
CTGGCTTTAA TCTACGGCAC TCCTAGAGCA GCCACTGTCA GAGCAAAGAC CAATGTCAAG 
CTGTGGGGCA TTGACAGAGA CAGCTACAGG AGAATACTCA TGGGAAGTAC TTTAAGAAAG 
AGGAAAATGT ATGAGGAATT CCTTAGCAAG GTGTCCATTT TGGAGTCTCT GGATAAATGG 
GAACGATTGA CAGTCGCTGA TGCACTTGAG ACAGTCCAGT TTGAAGATGG CCAGAAGATT 
GTGGTGCAGG GTCAACCAGG AGATGAGTTC TTCATAATTC TTGAGGGCTC GGCTGCGGTC 
TTGCAACGGC GGTCGGAGAA TGAAGAATTT GTGGAGGTTG GAAGACTCGC ACCGTCTGAC 
TACTTCGGAG AGATCGCTCT GCTCATGAAC CGTCCTCGTG CTGCCACCGT GGTTGCCCGC 
GGCCCGCTGA AGTGTGTAAA ACTCGACCGT CCTCGGTTCG AGCGTGTGCT CGGCCCCTGC 
TCAGATATCC TGAAGAGAAA CATCCAGCAG TACAACAGCT TCGTATCTCT GTCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164080.1
CDS	139..1278
Translation	MASSSTSSEEERSLRECERYVQKHNIQQLLKDCIVQLCTARPERPMAYLRDYFEKLELEEAKQMVSQQKSSSRSDSREDEVSPPMNPVVKGRRRRGAISAEVYTEEDATSYVRKVIPKDYKTMAALAKAIEKNVLFAHLDDNERSDIFDAMFSVTYIAGETVIQQGDEGDNFYVIDQGEMDVYVNNEWVTSIGEGGSFGELALIYGTPRAATVRAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALETVQFEDGQKIVVQGQPGDEFFIILEGSAAVLQRRSENEEFVEVGRLAPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Target ORF information:

RefSeq Version	XM_005164023.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a (prkar1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164023.3

ORF Insert Sequence:

ATGGCGTCCA GCAGTACGAG CAGTGAAGAA GAGAGGAGCT TGCGGGAGTG TGAGCGCTAC 
GTGCAGAAGC ACAACATCCA GCAGCTGCTG AAGGACTGCA TTGTGCAGCT GTGCACCGCC 
AGGCCTGAAA GACCCATGGC TTACCTCAGA GACTACTTCG AGAAGCTTGA GTTGGAGGAG 
GCTAAACAGA TGGTGAGCCA GCAGAAGTCC AGCTCTCGCT CAGATTCTCG GGAAGATGAG 
GTCTCTCCTC CTATGAACCC TGTGGTTAAA GGCCGACGGC GCCGTGGTGC CATCAGTGCA 
GAGGTCTACA CTGAGGAGGA CGCCACTTCT TATGTCAGAA AGGTCATTCC TAAAGACTAC 
AAAACTATGG CTGCCCTTGC TAAAGCAATT GAAAAGAATG TTCTTTTTGC TCATCTTGAC 
GACAATGAAA GAAGTGACAT ATTTGACGCC ATGTTCTCAG TCACCTACAT TGCAGGGGAG 
ACTGTCATTC AGCAAGGGGA TGAAGGAGAT AATTTTTACG TTATTGATCA AGGTGAAATG 
GATGTGTATG TGAACAATGA GTGGGTGACC AGTATTGGAG AAGGTGGCAG TTTTGGTGAA 
CTGGCTTTAA TCTACGGCAC TCCTAGAGCA GCCACTGTCA GAGCAAAGAC CAATGTCAAG 
CTGTGGGGCA TTGACAGAGA CAGCTACAGG AGAATACTCA TGGGAAGTAC TTTAAGAAAG 
AGGAAAATGT ATGAGGAATT CCTTAGCAAG GTGTCCATTT TGGAGTCTCT GGATAAATGG 
GAACGATTGA CAGTCGCTGA TGCACTTGAG ACAGTCCAGT TTGAAGATGG CCAGAAGATT 
GTGGTGCAGG GTCAACCAGG AGATGAGTTC TTCATAATTC TTGAGGGCTC GGCTGCGGTC 
TTGCAACGGC GGTCGGAGAA TGAAGAATTT GTGGAGGTTG GAAGACTCGC ACCGTCTGAC 
TACTTCGGAG AGATCGCTCT GCTCATGAAC CGTCCTCGTG CTGCCACCGT GGTTGCCCGC 
GGCCCGCTGA AGTGTGTAAA ACTCGACCGT CCTCGGTTCG AGCGTGTGCT CGGCCCCTGC 
TCAGATATCC TGAAGAGAAA CATCCAGCAG TACAACAGCT TCGTATCTCT GTCGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
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prkar1aa cDNA ORF clone, Danio rerio(zebrafish)

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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