tfr2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tfr2
Entrez Gene ID	494476
Full Name	transferrin receptor 2
Synonyms	zgc:123043
General protein information	Preferred Names transferrin receptor 2 Names transferrin receptor protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001009916.1	NP_001009916.1	transferrin receptor protein 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TFR2	NP_001171212.1
Rattus norvegicus (Norway rat)	Tfr2	NP_001099386.1
Mus musculus (house mouse)	Tfr2	NP_056614.3
Homo sapiens (human)	TFR2	NP_003218.2
Pan troglodytes (chimpanzee)	TFR2	XP_003318698.1
	TFR2	XP_001113151.1
Canis lupus familiaris (dog)	TFR2	XP_005621095.1
Danio rerio (zebrafish)	tfr2	NP_001009916.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Dynamic expression of long non-coding RNAs (lncRNAs) in adult zebrafish.
Kaushik K, Leonard VE, Kv S, Lalwani MK, Jalali S, Patowary A, Joshi A, Scaria V, Sivasubbu S
PloS one8(12)e83616(2013)

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
Yang D, Liu Q, Yang M, Wu H, Wang Q, Xiao J, Zhang Y
BMC genomics13319(2012 Jul)

Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN).
Foley JE, Yeh JR, Maeder ML, Reyon D, Sander JD, Peterson RT, Joung JK
PloS one4(2)e4348(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TfR2 is coexpressed with transferrin-a in the liver of the zebrafish embryo. Knockdown of TfR2 fails to produce anemia or a morphologic defect.
Title: Transferrin-a modulates hepcidin expression in zebrafish embryos.

The following tfr2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tfr2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01331	NM_001009916.1 Latest version!	Danio rerio transferrin receptor 2 (tfr2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tfr2 ( NM_001009916.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001009916.1 Danio rerio transferrin receptor 2 (tfr2), mRNA.

Price & Availability↑

CloneID	ODa01331
Clone ID Related Accession (Same CDS sequence)	NM_001009916.1
Accession Version	NM_001009916.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-10
Organism	Danio rerio(zebrafish)
Product	transferrin receptor protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY649365.1. ##Evidence-Data-START## Transcript exon combination :: AY649365.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGATGGACT CGGTCACAGG ACTGATTACT GGGGCAGCAG GGGGCGGAGC TCAGGCTAAT 
GAGGGCGTTG AGGAAGGGGG CGGAGTCAGG CGGAGAGCCC CTCCTATTGA GATGAAGCTG 
GTGCCACTGG AGGGGTCAGA TGTCACGCTC AACAGTGACA TCACCGCACT GATGCTGCAG 
CAGCACGTCA ACCGCAGGAA AGCAGCAGTT TACCTCACAC TGACCGGCCT GCTGATCTTC 
ACCACAGCGT TCCTGCTTGG ATACGTGGCG TTCCGCGGGA TGTGTCATCT GTGCCGCAGC 
GAGCCGGACA ACATTCCTCT GAGCGACGCA CTGGGAGACG ACCGGCAGCC TGAGGAAGTA 
GTTCTGTACA TGGCRGAGCT CAGAGAGATG CTGAAGAAAT ACCTAAAGGA GGAGCGCATC 
GAGAACACAC TCAGGCGAGT GAGCCGGGAG TCTCATCCTC CGGGCTCATC TGAAGGAAAC 
GCCATCGCCA GAGAAATCCT ACAGAACCTG CAGAACCTAG GCATGGACCA CACCTGGACT 
GACTCGCACT ACGCCACACT GCAGTTTCCC TCCAGGACGC ATCGGAACAC TCTGTGGTTG 
GTCGACTCTG AGGGGGCGGA GCTTGAAGAG ATTCGATTGG ACAGTGAGGG ATACTGTGCC 
TATAGTGCCA CTGGAACAGC CACGGGTGGT TTGGTGTATG TAAACTACGG TCGGCGTGAG 
GACTTTGATC AGCTGCGTGT GATGGGCGTG TCCCTCAACG GCTCCATCGC CATGGTGAGG 
GTCGGAGGGG GCGTGAGCTT CGCTGAGAAG GTGTGGCTTG CGCAGGAGGC GGGGCATGTG 
GGCGTTCTCA TCTACCCTGA TCCTGCCGAC GTTCCGCAAG ATCCACGGCG GCTCGGGCTC 
CATAGCAACG CCGTCATCTC AGAACACGTG CATTTGGGCT CTGGTGACCC GTTCACACCT 
GGGTTTCCCT CGTTTAACCA CACCCAGTTC CCATCCACAC AATCCTCAGG CCTGCCAATC 
ATTCCGGCTC AGCCAATCAG TGCCAATGTG GCTGCCAAAC TGCTGAGTCA GCTCTCAGGT 
GCAGTGTGCC CGCGGGGTTG GCAGGGTCGT CTGCCGTATG TTCAGTGTGT TCTGGGTCCG 
GGTTTCTCTG GTGTGGGTGT GCGGCAGCTG AAGATGGCCG TCTACAACAG YATGAAACCT 
GTGCTGCTCA ACAACATCTT CGCCTCCATT GAGGGAAAAG TGGAGCCCGA TCACTATATA 
ATAATCGGGG CCCAGAGGGA TGCGTGGGGC CCCGGAGCCG TGAAGTCTGG TGTGGGAACG 
GCCCTCCTGA TGGAGCTAGC AAGAACATTC AGCAACATGG TGGAAAACGG CTTTTCCCCG 
CGGCGCAGTC TGCTGTTCGT CAGCTGGGAT GGAGGAGAGT TTGGGAATGT TGGCGCCACC 
GAGTGGCTGG AGGGTTACCT GACGATGCTG CACCTGAAGG CTGTGGCGTA TTTCAGTCTG 
GATCAGGCCA TCCTGGGTGA TGATGTCCTG TCGGTGTACA GCAGTCCTCT TCTCTCTCAC 
CTCATCGAAG GAGCCATCAA ACAAGTGGAG CACCCCAAAC ACACAGGACA GTCCATCCTC 
TCTCTGGTGC AGCGGCAGGG TGGATGGAGG AACATAGTCA AGCCGTTGTA TCTGAACAGY 
GGCGCATACA GTTTCACCGC ATTYGGAGGA GTTCCTGCCA TGCAGCTGCA TTTCACTGAG 
GATKCTCGCT CTTACCCRTT CGTCAACACC CAGCTGGACA GTGTGGGGCG TCTGCAGGAA 
CTCCTGCAAG GGCGTCTGGG CTCGGTGGGT CGGGCTGTGG GTGAACTGGT GGGTCTGATG 
GTCCTCAAAC TGGCCCATGA TCACATCCTC CCGTTGGACG TGTCTTGCTA CAGCAGCAGC 
GCTCAGCAGC TCAGCTCCAC TCTCAGCCAG CACGCTGCAC AGCTACAGTC TCGAGGGTTG 
TCTCCGCAGT GGGTTTTCAG CGCACGCGGC GATTACAGCA GAGCGGCCAA AAACCTGCTG 
GAGGCCATCA AAAACAGCGA CACGCAGGAC GAAAAACTGG CCCGACTCTA CAACACACGC 
ATCATKAGGG TTGAGTACTA CTTTCTGTCT CAGTACGTGT CGGTGGTGGA GACGCCGTTC 
CGGCACGTGT TTCTGGGGCG TGGAGATCAC ACCATGAGCT CTCTGGCCGA TCACCTGAAC 
CAGCTGCGCT TGGAGCCGCA TCGCTTCGAT GAAGCCCGTT TCCGAAAACA GCTAGCGCTC 
TTCACATGGA CACTGCAGGG CGCTGCTAAT GCGCTCAGCG GAGACGTCTG GAACATCGAC 
AACCCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009916.1
CDS	1..2352
Translation	MMDSVTGLITGAAGGGAQANEGVEEGGGVRRRAPPIEMKLVPLEGSDVTLNSDITALMLQQHVNRRKAAVYLTLTGLLIFTTAFLLGYVAFRGMCHLCRSEPDNIPLSDALGDDRQPEEVVLYMnullELREMLKKYLKEERIENTLRRVSRESHPPGSSEGNAIAREILQNLQNLGMDHTWTDSHYATLQFPSRTHRNTLWLVDSEGAELEEIRLDSEGYCAYSATGTATGGLVYVNYGRREDFDQLRVMGVSLNGSIAMVRVGGGVSFAEKVWLAQEAGHVGVLIYPDPADVPQDPRRLGLHSNAVISEHVHLGSGDPFTPGFPSFNHTQFPSTQSSGLPIIPAQPISANVAAKLLSQLSGAVCPRGWQGRLPYVQCVLGPGFSGVGVRQLKMAVYNnullMKPVLLNNIFASIEGKVEPDHYIIIGAQRDAWGPGAVKSGVGTALLMELARTFSNMVENGFSPRRSLLFVSWDGGEFGNVGATEWLEGYLTMLHLKAVAYFSLDQAILGDDVLSVYSSPLLSHLIEGAIKQVEHPKHTGQSILSLVQRQGGWRNIVKPLYLNnullGAYSFTAnullGGVPAMQLHFTEDnullRSYnullFVNTQLDSVGRLQELLQGRLGSVGRAVGELVGLMVLKLAHDHILPLDVSCYSSSAQQLSSTLSQHAAQLQSRGLSPQWVFSARGDYSRAAKNLLEAIKNSDTQDEKLARLYNTRInullRVEYYFLSQYVSVVETPFRHVFLGRGDHTMSSLADHLNQLRLEPHRFDEARFRKQLALFTWTLQGAANALSGDVWNIDNPL

Target ORF information:

RefSeq Version	NM_001009916.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transferrin receptor 2 (tfr2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009916.1

ORF Insert Sequence:

ATGATGGACT CGGTCACAGG ACTGATTACT GGGGCAGCAG GGGGCGGAGC TCAGGCTAAT 
GAGGGCGTTG AGGAAGGGGG CGGAGTCAGG CGGAGAGCCC CTCCTATTGA GATGAAGCTG 
GTGCCACTGG AGGGGTCAGA TGTCACGCTC AACAGTGACA TCACCGCACT GATGCTGCAG 
CAGCACGTCA ACCGCAGGAA AGCAGCAGTT TACCTCACAC TGACCGGCCT GCTGATCTTC 
ACCACAGCGT TCCTGCTTGG ATACGTGGCG TTCCGCGGGA TGTGTCATCT GTGCCGCAGC 
GAGCCGGACA ACATTCCTCT GAGCGACGCA CTGGGAGACG ACCGGCAGCC TGAGGAAGTA 
GTTCTGTACA TGGCRGAGCT CAGAGAGATG CTGAAGAAAT ACCTAAAGGA GGAGCGCATC 
GAGAACACAC TCAGGCGAGT GAGCCGGGAG TCTCATCCTC CGGGCTCATC TGAAGGAAAC 
GCCATCGCCA GAGAAATCCT ACAGAACCTG CAGAACCTAG GCATGGACCA CACCTGGACT 
GACTCGCACT ACGCCACACT GCAGTTTCCC TCCAGGACGC ATCGGAACAC TCTGTGGTTG 
GTCGACTCTG AGGGGGCGGA GCTTGAAGAG ATTCGATTGG ACAGTGAGGG ATACTGTGCC 
TATAGTGCCA CTGGAACAGC CACGGGTGGT TTGGTGTATG TAAACTACGG TCGGCGTGAG 
GACTTTGATC AGCTGCGTGT GATGGGCGTG TCCCTCAACG GCTCCATCGC CATGGTGAGG 
GTCGGAGGGG GCGTGAGCTT CGCTGAGAAG GTGTGGCTTG CGCAGGAGGC GGGGCATGTG 
GGCGTTCTCA TCTACCCTGA TCCTGCCGAC GTTCCGCAAG ATCCACGGCG GCTCGGGCTC 
CATAGCAACG CCGTCATCTC AGAACACGTG CATTTGGGCT CTGGTGACCC GTTCACACCT 
GGGTTTCCCT CGTTTAACCA CACCCAGTTC CCATCCACAC AATCCTCAGG CCTGCCAATC 
ATTCCGGCTC AGCCAATCAG TGCCAATGTG GCTGCCAAAC TGCTGAGTCA GCTCTCAGGT 
GCAGTGTGCC CGCGGGGTTG GCAGGGTCGT CTGCCGTATG TTCAGTGTGT TCTGGGTCCG 
GGTTTCTCTG GTGTGGGTGT GCGGCAGCTG AAGATGGCCG TCTACAACAG YATGAAACCT 
GTGCTGCTCA ACAACATCTT CGCCTCCATT GAGGGAAAAG TGGAGCCCGA TCACTATATA 
ATAATCGGGG CCCAGAGGGA TGCGTGGGGC CCCGGAGCCG TGAAGTCTGG TGTGGGAACG 
GCCCTCCTGA TGGAGCTAGC AAGAACATTC AGCAACATGG TGGAAAACGG CTTTTCCCCG 
CGGCGCAGTC TGCTGTTCGT CAGCTGGGAT GGAGGAGAGT TTGGGAATGT TGGCGCCACC 
GAGTGGCTGG AGGGTTACCT GACGATGCTG CACCTGAAGG CTGTGGCGTA TTTCAGTCTG 
GATCAGGCCA TCCTGGGTGA TGATGTCCTG TCGGTGTACA GCAGTCCTCT TCTCTCTCAC 
CTCATCGAAG GAGCCATCAA ACAAGTGGAG CACCCCAAAC ACACAGGACA GTCCATCCTC 
TCTCTGGTGC AGCGGCAGGG TGGATGGAGG AACATAGTCA AGCCGTTGTA TCTGAACAGY 
GGCGCATACA GTTTCACCGC ATTYGGAGGA GTTCCTGCCA TGCAGCTGCA TTTCACTGAG 
GATKCTCGCT CTTACCCRTT CGTCAACACC CAGCTGGACA GTGTGGGGCG TCTGCAGGAA 
CTCCTGCAAG GGCGTCTGGG CTCGGTGGGT CGGGCTGTGG GTGAACTGGT GGGTCTGATG 
GTCCTCAAAC TGGCCCATGA TCACATCCTC CCGTTGGACG TGTCTTGCTA CAGCAGCAGC 
GCTCAGCAGC TCAGCTCCAC TCTCAGCCAG CACGCTGCAC AGCTACAGTC TCGAGGGTTG 
TCTCCGCAGT GGGTTTTCAG CGCACGCGGC GATTACAGCA GAGCGGCCAA AAACCTGCTG 
GAGGCCATCA AAAACAGCGA CACGCAGGAC GAAAAACTGG CCCGACTCTA CAACACACGC 
ATCATKAGGG TTGAGTACTA CTTTCTGTCT CAGTACGTGT CGGTGGTGGA GACGCCGTTC 
CGGCACGTGT TTCTGGGGCG TGGAGATCAC ACCATGAGCT CTCTGGCCGA TCACCTGAAC 
CAGCTGCGCT TGGAGCCGCA TCGCTTCGAT GAAGCCCGTT TCCGAAAACA GCTAGCGCTC 
TTCACATGGA CACTGCAGGG CGCTGCTAAT GCGCTCAGCG GAGACGTCTG GAACATCGAC 
AACCCGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Dynamic expression of long non-coding RNAs (lncRNAs) in adult zebrafish.
PloS one8(12)e83616(2013)
Kaushik K,Leonard VE,Kv S,Lalwani MK,Jalali S,Patowary A,Joshi A,Scaria V,Sivasubbu S

RNA-seq liver transcriptome analysis reveals an activated MHC-I pathway and an inhibited MHC-II pathway at the early stage of vaccine immunization in zebrafish.
BMC genomics13319(2012 Jul)
Yang D,Liu Q,Yang M,Wu H,Wang Q,Xiao J,Zhang Y

Rapid mutation of endogenous zebrafish genes using zinc finger nucleases made by Oligomerized Pool ENgineering (OPEN).
PloS one4(2)e4348(2009)
Foley JE,Yeh JR,Maeder ML,Reyon D,Sander JD,Peterson RT,Joung JK