hgfa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hgfa
Entrez Gene ID	493781
Full Name	hepatocyte growth factor a
Synonyms	hgf1,si:dkey-146j3.2
General protein information	Preferred Names hepatocyte growth factor a Names hepatocyte growth factor hepatocyte growth factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164742.2	XP_005164799.1	hepatocyte growth factor isoform X1
NM_001013274.1	NP_001013292.1	hepatocyte growth factor precursor
XM_005164742.3	XP_005164799.1	hepatocyte growth factor isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HGF	NP_001002964.1
Rattus norvegicus (Norway rat)	Hgf	NP_058713.1
	HGF	XP_001108226.2
Bos taurus (cattle)	HGF	NP_001026921.1
Homo sapiens (human)	HGF	NP_000592.3
Pan troglodytes (chimpanzee)	HGF	XP_519174.2
Mus musculus (house mouse)	Hgf	NP_034557.3
Xenopus tropicalis (tropical clawed frog)	hgf	XP_002933142.2
Gallus gallus (chicken)	HGF	NP_001025541.2
Danio rerio (zebrafish)	hgfa	NP_001013292.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

Hepatocyte growth factor signaling in intrapancreatic ductal cells drives pancreatic morphogenesis.
Anderson RM, Delous M, Bosch JA, Ye L, Robertson MA, Hesselson D, Stainier DY
PLoS genetics9(7)e1003650(2013)

Progranulin A-mediated MET signaling is essential for liver morphogenesis in zebrafish.
Li YH, Chen MH, Gong HY, Hu SY, Li YW, Lin GH, Lin CC, Liu W, Wu JL
The Journal of biological chemistry285(52)41001-9(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

hgf1 is transcribed during gastrulation while hgf1 and hgf2 are detectable in pharyngeal arches and swim bladder
Title: Hgf/c-met expression and functional analysis during zebrafish embryogenesis.

Loss-of-function experiments reveal that Met and its ligand, hepatocyte growth factor, are required for the correct morphogenesis of the hypaxial muscles in which met is expressed.
Title: Met and Hgf signaling controls hypaxial muscle and lateral line development in the zebrafish.

The following hgfa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hgfa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33289	NM_001013274.1 Latest version!	Danio rerio hepatocyte growth factor a (hgfa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa09370	XM_005164742.3 Latest version!	Danio rerio hepatocyte growth factor a (hgfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa09370	XM_005164742.2	Danio rerio hepatocyte growth factor a (hgfa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hgfa ( NM_001013274.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001013292.1 Danio rerio hepatocyte growth factor a (hgfa), mRNA.

Price & Availability↑

CloneID	ODa33289
Clone ID Related Accession (Same CDS sequence)	NM_001013274.1
Accession Version	NM_001013274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	hepatocyte growth factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX897731.7. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGATCGG ACATTATGTG GATGTATCAA GCTCTTCTCT TTGTCGTACT GACCGTGAAT 
GTGGATTGCA GAAAACAGAC ATTGCAGAGG TATCAGAAAA GTGAAAACAG CCGACTGCTT 
TGTACAGACT GTCCAGAATC CCCTCGAATT AGGAACCTGT CGCTGGAGGA ATGCGCACGC 
AAATGTAGCA AGAGCAAGAA GTCCTGCAGG GCATTCTACT TTGACCACAT AAACAGGAAA 
TGTCACTTTC TTCCTTTTGA CCGTTTCTCC GAAGGCGCGA AGAGAGAGCG AAAACCCAGT 
TGTGACCTTT ACGAGAAGAA AGACTATGTG AGGGAGTGCA TCATTGGGTC AGGTGTCAAC 
TACAAGGGGA GAAGGTCATT CACAAAGACT GGAATTACGT GCCAATCCTG GAATATGTCC 
GTCCCACATG AGCACAATTT TAAGCCTACC AGACACAAAA AGTCTGACCT ACGTCAGAAC 
TTTTGCCGAA ATCCAGACAA TGATCCAAAT GGGCCCTGGT GCTTCACTGA ACTCACTGAG 
ACCCGGCACC AGGATTGTGG ACTGCCTCAG TGTTCAGATG TGGAGTGTAT GAAATGTAAT 
GGGGAAACCT ACAGAGGGCC CATGGATCAT ACAGAGAGTG GGAAAGAGTG CCAGAGATGG 
GACTCGCAGA AACCTCATAA ACACACCTAC CAGCCTCACA GGCATGTAGG TAAAGGTCTG 
GATGACAACT TCTGCCGCAA TCCCAATAAT GATGTTCGTC CGTGGTGTTA CACGATGGAC 
AAAAACACCC CTTGGGAGTA CTGTAACATC AGTGTGTGTG ACTCAGATAG CGATGTGGAA 
GTGGAGGTGA CCAGCTCTTG TTTTCGGGGT CAAGGAGAGG GCTACAGGGG TACAGTCAAC 
GTGACCCCTG CAGGTGTGAC CTGTCAACGC TGGGACGCTC TCTCTCCTCA CATCCATTCA 
TACACTCCGC ACAACTATAA ATGCAAGGAT CTGAGGGAGA ACTATTGCAG AAATCCTGAT 
GGCTCTGAAA TTCCCTGGTG TTTCACCACA GACGCAAAAG TGCGCAAAGC TTTCTGTACC 
AACATTCCCA GATGCGAATC AGAGAGCTCT GACAGCACAG AATGTTATGA AGACAACGGA 
GAGAGTTATC GTGGCAATTT GTCGAAAACG AGATCTGGTA TTCCTTGCGG ACTCTGGTCT 
GACCACACAT TCAGGAGAGA CACCCGGTCA GCGAAGGCCA GCGCGGGTTT AGAGTTGAAC 
TTGTGCAGGA ATCCAGACAG AGATAAGCAC GGGCCGTGGT GCTACACGTC CAACTCCTCC 
ATTCCCTGGG ACTACTGCGG ACTGGAGCGC TGTGGAGGAC CAAAACCATC TTGCTTTATA 
CATAAAACCA CACGGATTGT TGGGGGAATG CGAGTGCAGC GGGCAGAGGA TGGAAGTTGG 
GTGGTCAGCA TTCAGAAAGG GAACAGACAC TGGTGCGGTG GCTCTCTCAT CAGAGAAGAA 
TGGGTTCTCA CTGATCAACA GTGCTTCTCC ACCTGCGTTC CTGACCTCTC CGAGTACACC 
GTGCAGGTGG GGCTTCTTCA TCTCAATGCA TCCGCCGGCA CGCAGGCTCT CCGAATCGCA 
CATGTGGTCT GCGGGCCCGA GGGATCCAAC TTGGCCCTGC TCAAACTCAC AACGCCTGCC 
CCTCTCTCTG AGCATGTGCG AACTGTTCAG CTTCCGGTCG CTGGCTGTGC TGTTGCAGAA 
GGCACTCTGT GTCTCATGTA TGGATGGGGA GACACTAAAG GACACGAGGG CAGTCTGAAG 
ATGGTGGGAC TTCCAATTGT CAGCAACAAG AGGTGTTCAC AAAGCCACAA TGGCATCCTG 
CCCATCACCG AGACCAAAAT CTGTGCTGGA GGCAAGAGAG ATCAGGGCGT TTGTGAGAAA 
GACTACGGTG GCCCTTTGGT GTGCCAGGAA GGAGAGAGCA AAGTGATAGT TGGCGTCAGC 
ATCAACGGCC GTGGTTGTGC AGTAGCGAGA CGTCCTGCTG TCTTCGTCAA CGTGGCCTTC 
TACTCTGAAT GGATCCGCAA GGTCTTCAAG TACTATTCAG ACATGGAAAT AAGTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013292.1
CDS	150..2249
Translation	MRSDIMWMYQALLFVVLTVNVDCRKQTLQRYQKSENSRLLCTDCPESPRIRNLSLEECARKCSKSKKSCRAFYFDHINRKCHFLPFDRFSEGAKRERKPSCDLYEKKDYVRECIIGSGVNYKGRRSFTKTGITCQSWNMSVPHEHNFKPTRHKKSDLRQNFCRNPDNDPNGPWCFTELTETRHQDCGLPQCSDVECMKCNGETYRGPMDHTESGKECQRWDSQKPHKHTYQPHRHVGKGLDDNFCRNPNNDVRPWCYTMDKNTPWEYCNISVCDSDSDVEVEVTSSCFRGQGEGYRGTVNVTPAGVTCQRWDALSPHIHSYTPHNYKCKDLRENYCRNPDGSEIPWCFTTDAKVRKAFCTNIPRCESESSDSTECYEDNGESYRGNLSKTRSGIPCGLWSDHTFRRDTRSAKASAGLELNLCRNPDRDKHGPWCYTSNSSIPWDYCGLERCGGPKPSCFIHKTTRIVGGMRVQRAEDGSWVVSIQKGNRHWCGGSLIREEWVLTDQQCFSTCVPDLSEYTVQVGLLHLNASAGTQALRIAHVVCGPEGSNLALLKLTTPAPLSEHVRTVQLPVAGCAVAEGTLCLMYGWGDTKGHEGSLKMVGLPIVSNKRCSQSHNGILPITETKICAGGKRDQGVCEKDYGGPLVCQEGESKVIVGVSINGRGCAVARRPAVFVNVAFYSEWIRKVFKYYSDMEISY

Target ORF information:

RefSeq Version	NM_001013274.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hepatocyte growth factor a (hgfa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013274.1

ORF Insert Sequence:

ATGAGATCGG ACATTATGTG GATGTATCAA GCTCTTCTCT TTGTCGTACT GACCGTGAAT 
GTGGATTGCA GAAAACAGAC ATTGCAGAGG TATCAGAAAA GTGAAAACAG CCGACTGCTT 
TGTACAGACT GTCCAGAATC CCCTCGAATT AGGAACCTGT CGCTGGAGGA ATGCGCACGC 
AAATGTAGCA AGAGCAAGAA GTCCTGCAGG GCATTCTACT TTGACCACAT AAACAGGAAA 
TGTCACTTTC TTCCTTTTGA CCGTTTCTCC GAAGGCGCGA AGAGAGAGCG AAAACCCAGT 
TGTGACCTTT ACGAGAAGAA AGACTATGTG AGGGAGTGCA TCATTGGGTC AGGTGTCAAC 
TACAAGGGGA GAAGGTCATT CACAAAGACT GGAATTACGT GCCAATCCTG GAATATGTCC 
GTCCCACATG AGCACAATTT TAAGCCTACC AGACACAAAA AGTCTGACCT ACGTCAGAAC 
TTTTGCCGAA ATCCAGACAA TGATCCAAAT GGGCCCTGGT GCTTCACTGA ACTCACTGAG 
ACCCGGCACC AGGATTGTGG ACTGCCTCAG TGTTCAGATG TGGAGTGTAT GAAATGTAAT 
GGGGAAACCT ACAGAGGGCC CATGGATCAT ACAGAGAGTG GGAAAGAGTG CCAGAGATGG 
GACTCGCAGA AACCTCATAA ACACACCTAC CAGCCTCACA GGCATGTAGG TAAAGGTCTG 
GATGACAACT TCTGCCGCAA TCCCAATAAT GATGTTCGTC CGTGGTGTTA CACGATGGAC 
AAAAACACCC CTTGGGAGTA CTGTAACATC AGTGTGTGTG ACTCAGATAG CGATGTGGAA 
GTGGAGGTGA CCAGCTCTTG TTTTCGGGGT CAAGGAGAGG GCTACAGGGG TACAGTCAAC 
GTGACCCCTG CAGGTGTGAC CTGTCAACGC TGGGACGCTC TCTCTCCTCA CATCCATTCA 
TACACTCCGC ACAACTATAA ATGCAAGGAT CTGAGGGAGA ACTATTGCAG AAATCCTGAT 
GGCTCTGAAA TTCCCTGGTG TTTCACCACA GACGCAAAAG TGCGCAAAGC TTTCTGTACC 
AACATTCCCA GATGCGAATC AGAGAGCTCT GACAGCACAG AATGTTATGA AGACAACGGA 
GAGAGTTATC GTGGCAATTT GTCGAAAACG AGATCTGGTA TTCCTTGCGG ACTCTGGTCT 
GACCACACAT TCAGGAGAGA CACCCGGTCA GCGAAGGCCA GCGCGGGTTT AGAGTTGAAC 
TTGTGCAGGA ATCCAGACAG AGATAAGCAC GGGCCGTGGT GCTACACGTC CAACTCCTCC 
ATTCCCTGGG ACTACTGCGG ACTGGAGCGC TGTGGAGGAC CAAAACCATC TTGCTTTATA 
CATAAAACCA CACGGATTGT TGGGGGAATG CGAGTGCAGC GGGCAGAGGA TGGAAGTTGG 
GTGGTCAGCA TTCAGAAAGG GAACAGACAC TGGTGCGGTG GCTCTCTCAT CAGAGAAGAA 
TGGGTTCTCA CTGATCAACA GTGCTTCTCC ACCTGCGTTC CTGACCTCTC CGAGTACACC 
GTGCAGGTGG GGCTTCTTCA TCTCAATGCA TCCGCCGGCA CGCAGGCTCT CCGAATCGCA 
CATGTGGTCT GCGGGCCCGA GGGATCCAAC TTGGCCCTGC TCAAACTCAC AACGCCTGCC 
CCTCTCTCTG AGCATGTGCG AACTGTTCAG CTTCCGGTCG CTGGCTGTGC TGTTGCAGAA 
GGCACTCTGT GTCTCATGTA TGGATGGGGA GACACTAAAG GACACGAGGG CAGTCTGAAG 
ATGGTGGGAC TTCCAATTGT CAGCAACAAG AGGTGTTCAC AAAGCCACAA TGGCATCCTG 
CCCATCACCG AGACCAAAAT CTGTGCTGGA GGCAAGAGAG ATCAGGGCGT TTGTGAGAAA 
GACTACGGTG GCCCTTTGGT GTGCCAGGAA GGAGAGAGCA AAGTGATAGT TGGCGTCAGC 
ATCAACGGCC GTGGTTGTGC AGTAGCGAGA CGTCCTGCTG TCTTCGTCAA CGTGGCCTTC 
TACTCTGAAT GGATCCGCAA GGTCTTCAAG TACTATTCAG ACATGGAAAT AAGTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hgfa ( XM_005164742.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164799.1 Danio rerio hepatocyte growth factor a (hgfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09370
Clone ID Related Accession (Same CDS sequence)	XM_005164742.2 , XM_005164742.3
Accession Version	XM_005164742.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	hepatocyte growth factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGG ACATTATGTG GATGTATCAA GCTCTTCTCT TTGTCGTACT GACCGTGAAT 
GTGGATTGCA GAAAACAGAC ATTGCAGAGG TATCAGAAAA GTGAAAACAG CCGACTGCTT 
TGTACAGACT GTCCAGAATC CCCTCGAATT AGGAACCTGT CGCTGGAGGA ATGCGCACGC 
AAATGTAGCA AGAGCAAGAA GTCCTGCAGG GCATTCTACT TTGACCACAT AAACAGGAAA 
TGTCACTTTC TTCCTTTTGA CCGTTTCTCC GAAGGCGCGA AGAGAGAGCG AAAACCCAGT 
TGTGACCTTT ACGAGAAGAA AGACTATGTG AGGGAGTGCA TCATTGGGTC AGGTGTCAAC 
TACAAGGGGA GAAGGTCATT CACAAAGACT GGAATTACGT GCCAATCCTG GAATATGTCC 
GTCCCACATG AGCACAATTT TAAGCCTACC AGACACAAAA AGTCTGACCT ACGTCAGAAC 
TTTTGCCGAA ATCCAGACAA TGATCCAAAT GGGCCCTGGT GCTTCACTGA ACTCACTGAG 
ACCCGGCACC AGGATTGTGG ACTGCCTCAG TGTTCAGATG TGGAGTGTAT GAAATGTAAT 
GGGGAAACCT ACAGAGGGCC CATGGATCAT ACAGAGAGTG GGAAAGAGTG CCAGAGATGG 
GACTCGCAGA AACCTCATAA ACACACCTAC CAGCCTCACA GGCATGTAGG TAAAGGTCTG 
GATGACAACT TCTGCCGCAA TCCCAATAAT GATGTTCGTC CGTGGTGTTA CACGATGGAC 
AAAAACACCC CTTGGGAGTA CTGTAACATC AGTGTGTGTG ACTCAGATAG CGATGTGGAA 
GTGGAGGTGA CCAGCTCTTG TTTTCGGGGT CAAGGAGAGG GCTACAGGGG TACAGTCAAC 
GTGACCCCTG CAGGTGTGAC CTGTCAACGC TGGGACGCTC TCTCTCCTCA CATCCATTCA 
TACACTCCGC ACAACTATAA ATGCAAGGAT CTGAGGGAGA ACTATTGCAG AAATCCTGAT 
GGCTCTGAAA TTCCCTGGTG TTTCACCACA GACGCAAAAG TGCGCAAAGC TTTCTGTACC 
AACATTCCCA GATGCGAATC AGAGAGCTCT GACAGCACAG AATGTTATGA AGACAACGGA 
GAGAGTTATC GTGGCAATTT GTCGAAAACG AGATCTGGTA TTCCTTGCGG ACTCTGGTCT 
GACCACACAT TCAGGAGAGA CACCCGGTCA GCGAAGGCCA GCGCGGGTTT AGAGTTGAAC 
TTGTGCAGGA ATCCAGACAG AGATAAGCAC GGGCCGTGGT GCTACACGTC CAACTCCTCC 
ATTCCCTGGG ACTACTGCGG ACTGGAGCGC TGTAAATCAA TGTCATCGGA TGACCATCAA 
ATGAGTGGAG GACCAAAACC ATCTTGCTTT ATACATAAAA CCACACGGAT TGTTGGGGGA 
ATGCGAGTGC AGCGGGCAGA GGATGGAAGT TGGGTGGTCA GCATTCAGAA AGGGAACAGA 
CACTGGTGCG GTGGCTCTCT CATCAGAGAA GAATGGGTTC TCACTGATCA ACAGTGCTTC 
TCCACCTGCG TTCCTGACCT CTCCGAGTAC ACCGTGCAGG TGGGGCTTCT TCATCTCAAT 
GCATCCGCCG GCACGCAGGC TCTCCGAATC GCACATGTGG TCTGCGGGCC CGAGGGATCC 
AACTTGGCCC TGCTCAAACT CACAACGCCT GCCCCTCTCT CTGAGCATGT GCGAACTGTT 
CAGCTTCCGG TCGCTGGCTG TGCTGTTGCA GAAGGCACTC TGTGTCTCAT GTATGGATGG 
GGAGACACTA AAGGCACAGG ACACGAGGGC AGTCTGAAGA TGGTGGGACT TCCAATTGTC 
AGCAACAAGA GGTGTTCACA AAGCCACAAT GGCATCCTGC CCATCACCGA GACCAAAATC 
TGTGCTGGAG GCAAGAGAGA TCAGGGCGTT TGTGAGAAAG ACTACGGTGG CCCTTTGGTG 
TGCCAGGAAG GAGAGAGCAA AGTGATAGTT GGCGTCAGCA TCAACGGCCG TGGTTGTGCA 
GTAGCGAGAC GTCCTGCTGT CTTCGTCAAC GTGGCCTTCT ACTCTGAATG GATCCGCAAG 
GTCTTCAAGT ACTATTCAGA CATGGAAATA AGTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164799.1
CDS	145..2283
Translation	MRSDIMWMYQALLFVVLTVNVDCRKQTLQRYQKSENSRLLCTDCPESPRIRNLSLEECARKCSKSKKSCRAFYFDHINRKCHFLPFDRFSEGAKRERKPSCDLYEKKDYVRECIIGSGVNYKGRRSFTKTGITCQSWNMSVPHEHNFKPTRHKKSDLRQNFCRNPDNDPNGPWCFTELTETRHQDCGLPQCSDVECMKCNGETYRGPMDHTESGKECQRWDSQKPHKHTYQPHRHVGKGLDDNFCRNPNNDVRPWCYTMDKNTPWEYCNISVCDSDSDVEVEVTSSCFRGQGEGYRGTVNVTPAGVTCQRWDALSPHIHSYTPHNYKCKDLRENYCRNPDGSEIPWCFTTDAKVRKAFCTNIPRCESESSDSTECYEDNGESYRGNLSKTRSGIPCGLWSDHTFRRDTRSAKASAGLELNLCRNPDRDKHGPWCYTSNSSIPWDYCGLERCKSMSSDDHQMSGGPKPSCFIHKTTRIVGGMRVQRAEDGSWVVSIQKGNRHWCGGSLIREEWVLTDQQCFSTCVPDLSEYTVQVGLLHLNASAGTQALRIAHVVCGPEGSNLALLKLTTPAPLSEHVRTVQLPVAGCAVAEGTLCLMYGWGDTKGTGHEGSLKMVGLPIVSNKRCSQSHNGILPITETKICAGGKRDQGVCEKDYGGPLVCQEGESKVIVGVSINGRGCAVARRPAVFVNVAFYSEWIRKVFKYYSDMEISY

Target ORF information:

RefSeq Version	XM_005164742.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hepatocyte growth factor a (hgfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164742.2

ORF Insert Sequence:

ATGAGATCGG ACATTATGTG GATGTATCAA GCTCTTCTCT TTGTCGTACT GACCGTGAAT 
GTGGATTGCA GAAAACAGAC ATTGCAGAGG TATCAGAAAA GTGAAAACAG CCGACTGCTT 
TGTACAGACT GTCCAGAATC CCCTCGAATT AGGAACCTGT CGCTGGAGGA ATGCGCACGC 
AAATGTAGCA AGAGCAAGAA GTCCTGCAGG GCATTCTACT TTGACCACAT AAACAGGAAA 
TGTCACTTTC TTCCTTTTGA CCGTTTCTCC GAAGGCGCGA AGAGAGAGCG AAAACCCAGT 
TGTGACCTTT ACGAGAAGAA AGACTATGTG AGGGAGTGCA TCATTGGGTC AGGTGTCAAC 
TACAAGGGGA GAAGGTCATT CACAAAGACT GGAATTACGT GCCAATCCTG GAATATGTCC 
GTCCCACATG AGCACAATTT TAAGCCTACC AGACACAAAA AGTCTGACCT ACGTCAGAAC 
TTTTGCCGAA ATCCAGACAA TGATCCAAAT GGGCCCTGGT GCTTCACTGA ACTCACTGAG 
ACCCGGCACC AGGATTGTGG ACTGCCTCAG TGTTCAGATG TGGAGTGTAT GAAATGTAAT 
GGGGAAACCT ACAGAGGGCC CATGGATCAT ACAGAGAGTG GGAAAGAGTG CCAGAGATGG 
GACTCGCAGA AACCTCATAA ACACACCTAC CAGCCTCACA GGCATGTAGG TAAAGGTCTG 
GATGACAACT TCTGCCGCAA TCCCAATAAT GATGTTCGTC CGTGGTGTTA CACGATGGAC 
AAAAACACCC CTTGGGAGTA CTGTAACATC AGTGTGTGTG ACTCAGATAG CGATGTGGAA 
GTGGAGGTGA CCAGCTCTTG TTTTCGGGGT CAAGGAGAGG GCTACAGGGG TACAGTCAAC 
GTGACCCCTG CAGGTGTGAC CTGTCAACGC TGGGACGCTC TCTCTCCTCA CATCCATTCA 
TACACTCCGC ACAACTATAA ATGCAAGGAT CTGAGGGAGA ACTATTGCAG AAATCCTGAT 
GGCTCTGAAA TTCCCTGGTG TTTCACCACA GACGCAAAAG TGCGCAAAGC TTTCTGTACC 
AACATTCCCA GATGCGAATC AGAGAGCTCT GACAGCACAG AATGTTATGA AGACAACGGA 
GAGAGTTATC GTGGCAATTT GTCGAAAACG AGATCTGGTA TTCCTTGCGG ACTCTGGTCT 
GACCACACAT TCAGGAGAGA CACCCGGTCA GCGAAGGCCA GCGCGGGTTT AGAGTTGAAC 
TTGTGCAGGA ATCCAGACAG AGATAAGCAC GGGCCGTGGT GCTACACGTC CAACTCCTCC 
ATTCCCTGGG ACTACTGCGG ACTGGAGCGC TGTAAATCAA TGTCATCGGA TGACCATCAA 
ATGAGTGGAG GACCAAAACC ATCTTGCTTT ATACATAAAA CCACACGGAT TGTTGGGGGA 
ATGCGAGTGC AGCGGGCAGA GGATGGAAGT TGGGTGGTCA GCATTCAGAA AGGGAACAGA 
CACTGGTGCG GTGGCTCTCT CATCAGAGAA GAATGGGTTC TCACTGATCA ACAGTGCTTC 
TCCACCTGCG TTCCTGACCT CTCCGAGTAC ACCGTGCAGG TGGGGCTTCT TCATCTCAAT 
GCATCCGCCG GCACGCAGGC TCTCCGAATC GCACATGTGG TCTGCGGGCC CGAGGGATCC 
AACTTGGCCC TGCTCAAACT CACAACGCCT GCCCCTCTCT CTGAGCATGT GCGAACTGTT 
CAGCTTCCGG TCGCTGGCTG TGCTGTTGCA GAAGGCACTC TGTGTCTCAT GTATGGATGG 
GGAGACACTA AAGGCACAGG ACACGAGGGC AGTCTGAAGA TGGTGGGACT TCCAATTGTC 
AGCAACAAGA GGTGTTCACA AAGCCACAAT GGCATCCTGC CCATCACCGA GACCAAAATC 
TGTGCTGGAG GCAAGAGAGA TCAGGGCGTT TGTGAGAAAG ACTACGGTGG CCCTTTGGTG 
TGCCAGGAAG GAGAGAGCAA AGTGATAGTT GGCGTCAGCA TCAACGGCCG TGGTTGTGCA 
GTAGCGAGAC GTCCTGCTGT CTTCGTCAAC GTGGCCTTCT ACTCTGAATG GATCCGCAAG 
GTCTTCAAGT ACTATTCAGA CATGGAAATA AGTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hgfa ( XM_005164742.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164799.1 Danio rerio hepatocyte growth factor a (hgfa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09370
Clone ID Related Accession (Same CDS sequence)	XM_005164742.2 , XM_005164742.3
Accession Version	XM_005164742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	hepatocyte growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATCGG ACATTATGTG GATGTATCAA GCTCTTCTCT TTGTCGTACT GACCGTGAAT 
GTGGATTGCA GAAAACAGAC ATTGCAGAGG TATCAGAAAA GTGAAAACAG CCGACTGCTT 
TGTACAGACT GTCCAGAATC CCCTCGAATT AGGAACCTGT CGCTGGAGGA ATGCGCACGC 
AAATGTAGCA AGAGCAAGAA GTCCTGCAGG GCATTCTACT TTGACCACAT AAACAGGAAA 
TGTCACTTTC TTCCTTTTGA CCGTTTCTCC GAAGGCGCGA AGAGAGAGCG AAAACCCAGT 
TGTGACCTTT ACGAGAAGAA AGACTATGTG AGGGAGTGCA TCATTGGGTC AGGTGTCAAC 
TACAAGGGGA GAAGGTCATT CACAAAGACT GGAATTACGT GCCAATCCTG GAATATGTCC 
GTCCCACATG AGCACAATTT TAAGCCTACC AGACACAAAA AGTCTGACCT ACGTCAGAAC 
TTTTGCCGAA ATCCAGACAA TGATCCAAAT GGGCCCTGGT GCTTCACTGA ACTCACTGAG 
ACCCGGCACC AGGATTGTGG ACTGCCTCAG TGTTCAGATG TGGAGTGTAT GAAATGTAAT 
GGGGAAACCT ACAGAGGGCC CATGGATCAT ACAGAGAGTG GGAAAGAGTG CCAGAGATGG 
GACTCGCAGA AACCTCATAA ACACACCTAC CAGCCTCACA GGCATGTAGG TAAAGGTCTG 
GATGACAACT TCTGCCGCAA TCCCAATAAT GATGTTCGTC CGTGGTGTTA CACGATGGAC 
AAAAACACCC CTTGGGAGTA CTGTAACATC AGTGTGTGTG ACTCAGATAG CGATGTGGAA 
GTGGAGGTGA CCAGCTCTTG TTTTCGGGGT CAAGGAGAGG GCTACAGGGG TACAGTCAAC 
GTGACCCCTG CAGGTGTGAC CTGTCAACGC TGGGACGCTC TCTCTCCTCA CATCCATTCA 
TACACTCCGC ACAACTATAA ATGCAAGGAT CTGAGGGAGA ACTATTGCAG AAATCCTGAT 
GGCTCTGAAA TTCCCTGGTG TTTCACCACA GACGCAAAAG TGCGCAAAGC TTTCTGTACC 
AACATTCCCA GATGCGAATC AGAGAGCTCT GACAGCACAG AATGTTATGA AGACAACGGA 
GAGAGTTATC GTGGCAATTT GTCGAAAACG AGATCTGGTA TTCCTTGCGG ACTCTGGTCT 
GACCACACAT TCAGGAGAGA CACCCGGTCA GCGAAGGCCA GCGCGGGTTT AGAGTTGAAC 
TTGTGCAGGA ATCCAGACAG AGATAAGCAC GGGCCGTGGT GCTACACGTC CAACTCCTCC 
ATTCCCTGGG ACTACTGCGG ACTGGAGCGC TGTAAATCAA TGTCATCGGA TGACCATCAA 
ATGAGTGGAG GACCAAAACC ATCTTGCTTT ATACATAAAA CCACACGGAT TGTTGGGGGA 
ATGCGAGTGC AGCGGGCAGA GGATGGAAGT TGGGTGGTCA GCATTCAGAA AGGGAACAGA 
CACTGGTGCG GTGGCTCTCT CATCAGAGAA GAATGGGTTC TCACTGATCA ACAGTGCTTC 
TCCACCTGCG TTCCTGACCT CTCCGAGTAC ACCGTGCAGG TGGGGCTTCT TCATCTCAAT 
GCATCCGCCG GCACGCAGGC TCTCCGAATC GCACATGTGG TCTGCGGGCC CGAGGGATCC 
AACTTGGCCC TGCTCAAACT CACAACGCCT GCCCCTCTCT CTGAGCATGT GCGAACTGTT 
CAGCTTCCGG TCGCTGGCTG TGCTGTTGCA GAAGGCACTC TGTGTCTCAT GTATGGATGG 
GGAGACACTA AAGGCACAGG ACACGAGGGC AGTCTGAAGA TGGTGGGACT TCCAATTGTC 
AGCAACAAGA GGTGTTCACA AAGCCACAAT GGCATCCTGC CCATCACCGA GACCAAAATC 
TGTGCTGGAG GCAAGAGAGA TCAGGGCGTT TGTGAGAAAG ACTACGGTGG CCCTTTGGTG 
TGCCAGGAAG GAGAGAGCAA AGTGATAGTT GGCGTCAGCA TCAACGGCCG TGGTTGTGCA 
GTAGCGAGAC GTCCTGCTGT CTTCGTCAAC GTGGCCTTCT ACTCTGAATG GATCCGCAAG 
GTCTTCAAGT ACTATTCAGA CATGGAAATA AGTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164799.1
CDS	1076..3214
Translation	MRSDIMWMYQALLFVVLTVNVDCRKQTLQRYQKSENSRLLCTDCPESPRIRNLSLEECARKCSKSKKSCRAFYFDHINRKCHFLPFDRFSEGAKRERKPSCDLYEKKDYVRECIIGSGVNYKGRRSFTKTGITCQSWNMSVPHEHNFKPTRHKKSDLRQNFCRNPDNDPNGPWCFTELTETRHQDCGLPQCSDVECMKCNGETYRGPMDHTESGKECQRWDSQKPHKHTYQPHRHVGKGLDDNFCRNPNNDVRPWCYTMDKNTPWEYCNISVCDSDSDVEVEVTSSCFRGQGEGYRGTVNVTPAGVTCQRWDALSPHIHSYTPHNYKCKDLRENYCRNPDGSEIPWCFTTDAKVRKAFCTNIPRCESESSDSTECYEDNGESYRGNLSKTRSGIPCGLWSDHTFRRDTRSAKASAGLELNLCRNPDRDKHGPWCYTSNSSIPWDYCGLERCKSMSSDDHQMSGGPKPSCFIHKTTRIVGGMRVQRAEDGSWVVSIQKGNRHWCGGSLIREEWVLTDQQCFSTCVPDLSEYTVQVGLLHLNASAGTQALRIAHVVCGPEGSNLALLKLTTPAPLSEHVRTVQLPVAGCAVAEGTLCLMYGWGDTKGTGHEGSLKMVGLPIVSNKRCSQSHNGILPITETKICAGGKRDQGVCEKDYGGPLVCQEGESKVIVGVSINGRGCAVARRPAVFVNVAFYSEWIRKVFKYYSDMEISY

Target ORF information:

RefSeq Version	XM_005164742.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hepatocyte growth factor a (hgfa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164742.3

ORF Insert Sequence:

ATGAGATCGG ACATTATGTG GATGTATCAA GCTCTTCTCT TTGTCGTACT GACCGTGAAT 
GTGGATTGCA GAAAACAGAC ATTGCAGAGG TATCAGAAAA GTGAAAACAG CCGACTGCTT 
TGTACAGACT GTCCAGAATC CCCTCGAATT AGGAACCTGT CGCTGGAGGA ATGCGCACGC 
AAATGTAGCA AGAGCAAGAA GTCCTGCAGG GCATTCTACT TTGACCACAT AAACAGGAAA 
TGTCACTTTC TTCCTTTTGA CCGTTTCTCC GAAGGCGCGA AGAGAGAGCG AAAACCCAGT 
TGTGACCTTT ACGAGAAGAA AGACTATGTG AGGGAGTGCA TCATTGGGTC AGGTGTCAAC 
TACAAGGGGA GAAGGTCATT CACAAAGACT GGAATTACGT GCCAATCCTG GAATATGTCC 
GTCCCACATG AGCACAATTT TAAGCCTACC AGACACAAAA AGTCTGACCT ACGTCAGAAC 
TTTTGCCGAA ATCCAGACAA TGATCCAAAT GGGCCCTGGT GCTTCACTGA ACTCACTGAG 
ACCCGGCACC AGGATTGTGG ACTGCCTCAG TGTTCAGATG TGGAGTGTAT GAAATGTAAT 
GGGGAAACCT ACAGAGGGCC CATGGATCAT ACAGAGAGTG GGAAAGAGTG CCAGAGATGG 
GACTCGCAGA AACCTCATAA ACACACCTAC CAGCCTCACA GGCATGTAGG TAAAGGTCTG 
GATGACAACT TCTGCCGCAA TCCCAATAAT GATGTTCGTC CGTGGTGTTA CACGATGGAC 
AAAAACACCC CTTGGGAGTA CTGTAACATC AGTGTGTGTG ACTCAGATAG CGATGTGGAA 
GTGGAGGTGA CCAGCTCTTG TTTTCGGGGT CAAGGAGAGG GCTACAGGGG TACAGTCAAC 
GTGACCCCTG CAGGTGTGAC CTGTCAACGC TGGGACGCTC TCTCTCCTCA CATCCATTCA 
TACACTCCGC ACAACTATAA ATGCAAGGAT CTGAGGGAGA ACTATTGCAG AAATCCTGAT 
GGCTCTGAAA TTCCCTGGTG TTTCACCACA GACGCAAAAG TGCGCAAAGC TTTCTGTACC 
AACATTCCCA GATGCGAATC AGAGAGCTCT GACAGCACAG AATGTTATGA AGACAACGGA 
GAGAGTTATC GTGGCAATTT GTCGAAAACG AGATCTGGTA TTCCTTGCGG ACTCTGGTCT 
GACCACACAT TCAGGAGAGA CACCCGGTCA GCGAAGGCCA GCGCGGGTTT AGAGTTGAAC 
TTGTGCAGGA ATCCAGACAG AGATAAGCAC GGGCCGTGGT GCTACACGTC CAACTCCTCC 
ATTCCCTGGG ACTACTGCGG ACTGGAGCGC TGTAAATCAA TGTCATCGGA TGACCATCAA 
ATGAGTGGAG GACCAAAACC ATCTTGCTTT ATACATAAAA CCACACGGAT TGTTGGGGGA 
ATGCGAGTGC AGCGGGCAGA GGATGGAAGT TGGGTGGTCA GCATTCAGAA AGGGAACAGA 
CACTGGTGCG GTGGCTCTCT CATCAGAGAA GAATGGGTTC TCACTGATCA ACAGTGCTTC 
TCCACCTGCG TTCCTGACCT CTCCGAGTAC ACCGTGCAGG TGGGGCTTCT TCATCTCAAT 
GCATCCGCCG GCACGCAGGC TCTCCGAATC GCACATGTGG TCTGCGGGCC CGAGGGATCC 
AACTTGGCCC TGCTCAAACT CACAACGCCT GCCCCTCTCT CTGAGCATGT GCGAACTGTT 
CAGCTTCCGG TCGCTGGCTG TGCTGTTGCA GAAGGCACTC TGTGTCTCAT GTATGGATGG 
GGAGACACTA AAGGCACAGG ACACGAGGGC AGTCTGAAGA TGGTGGGACT TCCAATTGTC 
AGCAACAAGA GGTGTTCACA AAGCCACAAT GGCATCCTGC CCATCACCGA GACCAAAATC 
TGTGCTGGAG GCAAGAGAGA TCAGGGCGTT TGTGAGAAAG ACTACGGTGG CCCTTTGGTG 
TGCCAGGAAG GAGAGAGCAA AGTGATAGTT GGCGTCAGCA TCAACGGCCG TGGTTGTGCA 
GTAGCGAGAC GTCCTGCTGT CTTCGTCAAC GTGGCCTTCT ACTCTGAATG GATCCGCAAG 
GTCTTCAAGT ACTATTCAGA CATGGAAATA AGTTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

Hepatocyte growth factor signaling in intrapancreatic ductal cells drives pancreatic morphogenesis.
PLoS genetics9(7)e1003650(2013)
Anderson RM,Delous M,Bosch JA,Ye L,Robertson MA,Hesselson D,Stainier DY

Progranulin A-mediated MET signaling is essential for liver morphogenesis in zebrafish.
The Journal of biological chemistry285(52)41001-9(2010 Dec)
Li YH,Chen MH,Gong HY,Hu SY,Li YW,Lin GH,Lin CC,Liu W,Wu JL

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hgfa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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