ext2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ext2
Entrez Gene ID	493780
Full Name	exostosin glycosyltransferase 2
Synonyms	wu:fc14g07
General protein information	Preferred Names exostosin glycosyltransferase 2 Names exostosin-2 dackel dak exostoses (multiple) 2 ext2a fc14g07
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008400.1	NP_001008400.1	exostosin-2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ext2	NP_725536.1
Canis lupus familiaris (dog)	EXT2	XP_005631368.1
Rattus norvegicus (Norway rat)	Ext2	NP_001101221.1
Danio rerio (zebrafish)	ext2	NP_001008400.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000081	XP_311070.5
Homo sapiens (human)	EXT2	NP_001171554.1
Pan troglodytes (chimpanzee)	EXT2	XP_003313041.1
	EXT2	XP_001106468.2
Gallus gallus (chicken)	EXT2	NP_001026520.1
Xenopus tropicalis (tropical clawed frog)	ext2	NP_001120887.1
Bos taurus (cattle)	EXT2	NP_803462.2
Mus musculus (house mouse)	Ext2	NP_034293.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Volpi S, Yamazaki Y, Brauer PM, van Rooijen E, Hayashida A, Slavotinek A, Sun Kuehn H, Di Rocco M, Rivolta C, Bortolomai I, Du L, Felgentreff K, Ott de Bruin L, Hayashida K, Freedman G, Marcovecchio GE, Capuder K, Rath P, Luche N, Hagedorn EJ, Buoncompagni A, Royer-Bertrand B, Giliani S, Poliani PL, Imberti L, Dobbs K, Poulain FE, Martini A, Manis J, Linhardt RJ, Bosticardo M, Rosenzweig SD, Lee H, Puck JM, Z??iga-Pfl?cker JC, Zon L, Park PW, Superti-Furga A, Notarangelo LD
The Journal of experimental medicine214(3)623-637(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Heparan Sulfate Proteoglycans Regulate Fgf Signaling and Cell Polarity during Collective Cell Migration.
Venero Galanternik M, Kramer KL, Piotrowski T
Cell reports(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observations in the ext2-null fish might explain the musculoskeletal defects that are often observed in MO patients
Title: Possible effects of EXT2 on mesenchymal differentiation--lessons from the zebrafish.

analysis of chondroitin sulfate and heparan sulfate biosynthesis in zebrafish larvae homozygous for mutations in ext2, extl3, uxs1, or b3gat3
Title: On the roles and regulation of chondroitin sulfate and heparan sulfate in zebrafish pharyngeal cartilage morphogenesis.

We conclude that ext2 is involved in Fgf and Wnt signaling but not in Hh signaling, revealing an unexpected specificity for ext2 in signaling pathways during embryonic development.
Title: Zebrafish Ext2 is necessary for Fgf and Wnt signaling, but not for Hh signaling.

Regulation of zebrafish skeletogenesis by ext2/dak and papst1/pic is reported.
Title: Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher.

Ext2 and extl3 are differentially required for Fgf10, but not Fgf4, signalling during limb development.
Title: HSPG synthesis by zebrafish Ext2 and Extl3 is required for Fgf10 signalling during limb development.

The following ext2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ext2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02808	NM_001008400.1 Latest version!	Danio rerio exostosin glycosyltransferase 2 (ext2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ext2 ( NM_001008400.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001008400.1 Danio rerio exostosin glycosyltransferase 2 (ext2), mRNA.

Price & Availability↑

CloneID	ODa02808
Clone ID Related Accession (Same CDS sequence)	NM_001008400.1
Accession Version	NM_001008400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	exostosin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY786508.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY786508.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTGTGCAT CAGGGAAGTA CGGTTCACGG GGCCCTGCTC TCATACCCCG CATGAAGACT 
AAACACCGGA TCTACTACAT CACCCTCTTC TCTGTGGTCT TGCTGGGATT GATCGCAACA 
GGGATGTTCC AGTTCTGGCC TCACTCCATT GAGTCTTCAG CTGAATGGAG TCTGGACCGC 
CGCAGTGTGC ACGATGCTCC TTTGGTCAGG ATTTCTGTGA ACAGTCCTAT TCCTGGGAGG 
GGTGACCTGA GCTGCCGGAT GCACACCTGC TTTGATGTTT ATCGCTGTGG ATATAATCCT 
AAGAACAAAA TCAAGGTGTA CATCTATCCA CTTCAGAGGT TTGTGGATGA AGTAGGAGTG 
CCCATCAGTA GCACTGGCCT GTCTCGAGAA TACAATGACC TGCTGAGCGC CATCTCGGAC 
AGTGACTTCT ACACTGATGA TGTGTCCAGA GCGTGTCTGT TTGTACCTTC TATTGATGTA 
CTGAATCAGA ACTCTCTGCG TATTCGAGAA ACTGCTCAGG CACTGGCCAT GCTGCCYAGG 
TGGGACAAAG GCATGAACCA CCTGCTGTTT AAYATGCTTC CTGGAGGACC TCCTGACTAC 
AATACAGCTC TGGATGTGCC GAGAGACAGA GCCCTGCTGG CTGGGGGAGG CTTCTCCACA 
TGGACCTACA GACAAGGTTA TGATGTCAGC ATACCTGTGT ATAGCCCTTT ATCTGCTGAG 
GTGGACCTTC CTGAGAGGCA ACCCGGTCCT CGGCGCTACT TCATTCTGTC ATCTCAGACA 
GCCATTCATA GGGAATACCG TGTGGAACTG GAAAGGCTGA AGGATGAGAA TGGGGAGGCC 
CTGCTCCTGT TAGACAAATG CAGTAATCTC TCTCAGGGTT TGACCTCGGT TAGAAAGCGC 
TGCTATAAAG GACAGGTGTA CGACTACCCA CAAATTTTAC AGGAGTCGTC ATTCTGTGTG 
GTTCTGCGTG GGGCTCGATT AGGCCAAGCC ACGCTCAGTG ACGTGCTGCA AGCGGGATGT 
GTCCCTGTCA TCATGGCTGA TTCTTATATT CTGCCTTTCT CTGAAGTGCT GGACTGGAAA 
AGGGCGTCTG TTGTCATTCC AGAAGAAAAG TTGCCAGAGA TGTACACCAT TTTGAAGAGC 
ATACCTCACA GACAAGTGGA GGAGATGCAG AGACAGGCTC GCTGGTTTTG GGAAGCCTAC 
TTCAGCTCCA TGAAGGCTAT TGGCATGACG ACTCTCCAGA TCATTAATGA TCGCATTTAC 
CCWTATGCAG CTCATACCTA TGAAGAATGG AACAATCCTC CTGTGGTTAA ATGGTCGAGT 
GTGAACAGTC CACTTTTCCT TCCTCTCATA CCGCCACGCT CTCCTGGGTT CACAGCTGTC 
GTGTTGACTT ATGATCGTAT TGAAAGCCTT TTTAGGGTCA TCACTGAGAT CTCCAAGGTG 
CCCAGTTTGG CCAAACTACT GGTGGTGTGG AACAACCAGA ACAAAAGCCC ACCTGAAGAG 
TCGCTGTGGC CCAAAGTCGC AGTGCCTCTT AAAGTGGTCC GCACCAAAGA AAACAAGCTC 
AGCAATCGCT TCTTTCCGTT TGATGAGATC GAGACAGAAG CTGTCCTGGC CATTGACGAT 
GACATCATCA TGTTAACCTC AGATGAGCTA CAGTTTGGAT ATGAGGTATG GCGTGAGTTC 
CCTGACAGGC TGGTTGGATA TCCTGGTCGG CTCCATTTAT GGGATCATGA AATGGGCAAA 
TGGAAATACG AATCTGAATG GACTAATGAA GTATCCATGG TCTTGACTGG TGCTGCTTTC 
TACCATAAGT ATTTCAACTA TCTCTACACC TATAAAATGC CTGGAGACAT CAAGAACTGG 
GTGGATGCTC ACATGAACTG TGAAGATATT GCTATGAACT TCCTTGTGGC CAACATCACT 
GGAAAAGCAC CAATAAAGGT TACTCCTCGG AAGAAATTTA AATGCCCGGA GTGCACAGCA 
ATTGATGGGT TGTCCTTGGA CCAGACACAC ATGGTGGAGA GGTCTGAGTG CATAAACAAG 
TTCGCCTCTG TGTTCGGCAC CATGCCTCTG AAGGTTGTCG AGCACCGTGC TGATCCTGTA 
CTCTACAAAG ACGACTTCCC AGAGAAACTC AAAAGTTTCC CCAACATTGG CAGTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008400.1
CDS	193..2352
Translation	MCASGKYGSRGPALIPRMKTKHRIYYITLFSVVLLGLIATGMFQFWPHSIESSAEWSLDRRSVHDAPLVRISVNSPIPGRGDLSCRMHTCFDVYRCGYNPKNKIKVYIYPLQRFVDEVGVPISSTGLSREYNDLLSAISDSDFYTDDVSRACLFVPSIDVLNQNSLRIRETAQALAMLnullRWDKGMNHLLFnullMLPGGPPDYNTALDVPRDRALLAGGGFSTWTYRQGYDVSIPVYSPLSAEVDLPERQPGPRRYFILSSQTAIHREYRVELERLKDENGEALLLLDKCSNLSQGLTSVRKRCYKGQVYDYPQILQESSFCVVLRGARLGQATLSDVLQAGCVPVIMADSYILPFSEVLDWKRASVVIPEEKLPEMYTILKSIPHRQVEEMQRQARWFWEAYFSSMKAIGMTTLQIINDRIYnullYAAHTYEEWNNPPVVKWSSVNSPLFLPLIPPRSPGFTAVVLTYDRIESLFRVITEISKVPSLAKLLVVWNNQNKSPPEESLWPKVAVPLKVVRTKENKLSNRFFPFDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHEMGKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANITGKAPIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL

Target ORF information:

RefSeq Version	NM_001008400.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio exostosin glycosyltransferase 2 (ext2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008400.1

ORF Insert Sequence:

ATGTGTGCAT CAGGGAAGTA CGGTTCACGG GGCCCTGCTC TCATACCCCG CATGAAGACT 
AAACACCGGA TCTACTACAT CACCCTCTTC TCTGTGGTCT TGCTGGGATT GATCGCAACA 
GGGATGTTCC AGTTCTGGCC TCACTCCATT GAGTCTTCAG CTGAATGGAG TCTGGACCGC 
CGCAGTGTGC ACGATGCTCC TTTGGTCAGG ATTTCTGTGA ACAGTCCTAT TCCTGGGAGG 
GGTGACCTGA GCTGCCGGAT GCACACCTGC TTTGATGTTT ATCGCTGTGG ATATAATCCT 
AAGAACAAAA TCAAGGTGTA CATCTATCCA CTTCAGAGGT TTGTGGATGA AGTAGGAGTG 
CCCATCAGTA GCACTGGCCT GTCTCGAGAA TACAATGACC TGCTGAGCGC CATCTCGGAC 
AGTGACTTCT ACACTGATGA TGTGTCCAGA GCGTGTCTGT TTGTACCTTC TATTGATGTA 
CTGAATCAGA ACTCTCTGCG TATTCGAGAA ACTGCTCAGG CACTGGCCAT GCTGCCYAGG 
TGGGACAAAG GCATGAACCA CCTGCTGTTT AAYATGCTTC CTGGAGGACC TCCTGACTAC 
AATACAGCTC TGGATGTGCC GAGAGACAGA GCCCTGCTGG CTGGGGGAGG CTTCTCCACA 
TGGACCTACA GACAAGGTTA TGATGTCAGC ATACCTGTGT ATAGCCCTTT ATCTGCTGAG 
GTGGACCTTC CTGAGAGGCA ACCCGGTCCT CGGCGCTACT TCATTCTGTC ATCTCAGACA 
GCCATTCATA GGGAATACCG TGTGGAACTG GAAAGGCTGA AGGATGAGAA TGGGGAGGCC 
CTGCTCCTGT TAGACAAATG CAGTAATCTC TCTCAGGGTT TGACCTCGGT TAGAAAGCGC 
TGCTATAAAG GACAGGTGTA CGACTACCCA CAAATTTTAC AGGAGTCGTC ATTCTGTGTG 
GTTCTGCGTG GGGCTCGATT AGGCCAAGCC ACGCTCAGTG ACGTGCTGCA AGCGGGATGT 
GTCCCTGTCA TCATGGCTGA TTCTTATATT CTGCCTTTCT CTGAAGTGCT GGACTGGAAA 
AGGGCGTCTG TTGTCATTCC AGAAGAAAAG TTGCCAGAGA TGTACACCAT TTTGAAGAGC 
ATACCTCACA GACAAGTGGA GGAGATGCAG AGACAGGCTC GCTGGTTTTG GGAAGCCTAC 
TTCAGCTCCA TGAAGGCTAT TGGCATGACG ACTCTCCAGA TCATTAATGA TCGCATTTAC 
CCWTATGCAG CTCATACCTA TGAAGAATGG AACAATCCTC CTGTGGTTAA ATGGTCGAGT 
GTGAACAGTC CACTTTTCCT TCCTCTCATA CCGCCACGCT CTCCTGGGTT CACAGCTGTC 
GTGTTGACTT ATGATCGTAT TGAAAGCCTT TTTAGGGTCA TCACTGAGAT CTCCAAGGTG 
CCCAGTTTGG CCAAACTACT GGTGGTGTGG AACAACCAGA ACAAAAGCCC ACCTGAAGAG 
TCGCTGTGGC CCAAAGTCGC AGTGCCTCTT AAAGTGGTCC GCACCAAAGA AAACAAGCTC 
AGCAATCGCT TCTTTCCGTT TGATGAGATC GAGACAGAAG CTGTCCTGGC CATTGACGAT 
GACATCATCA TGTTAACCTC AGATGAGCTA CAGTTTGGAT ATGAGGTATG GCGTGAGTTC 
CCTGACAGGC TGGTTGGATA TCCTGGTCGG CTCCATTTAT GGGATCATGA AATGGGCAAA 
TGGAAATACG AATCTGAATG GACTAATGAA GTATCCATGG TCTTGACTGG TGCTGCTTTC 
TACCATAAGT ATTTCAACTA TCTCTACACC TATAAAATGC CTGGAGACAT CAAGAACTGG 
GTGGATGCTC ACATGAACTG TGAAGATATT GCTATGAACT TCCTTGTGGC CAACATCACT 
GGAAAAGCAC CAATAAAGGT TACTCCTCGG AAGAAATTTA AATGCCCGGA GTGCACAGCA 
ATTGATGGGT TGTCCTTGGA CCAGACACAC ATGGTGGAGA GGTCTGAGTG CATAAACAAG 
TTCGCCTCTG TGTTCGGCAC CATGCCTCTG AAGGTTGTCG AGCACCGTGC TGATCCTGTA 
CTCTACAAAG ACGACTTCCC AGAGAAACTC AAAAGTTTCC CCAACATTGG CAGTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

The Journal of experimental medicine214(3)623-637(2017 Mar)
Volpi S,Yamazaki Y,Brauer PM,van Rooijen E,Hayashida A,Slavotinek A,Sun Kuehn H,Di Rocco M,Rivolta C,Bortolomai I,Du L,Felgentreff K,Ott de Bruin L,Hayashida K,Freedman G,Marcovecchio GE,Capuder K,Rath P,Luche N,Hagedorn EJ,Buoncompagni A,Royer-Bertrand B,Giliani S,Poliani PL,Imberti L,Dobbs K,Poulain FE,Martini A,Manis J,Linhardt RJ,Bosticardo M,Rosenzweig SD,Lee H,Puck JM,Z??iga-Pfl?cker JC,Zon L,Park PW,Superti-Furga A,Notarangelo LD

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Heparan Sulfate Proteoglycans Regulate Fgf Signaling and Cell Polarity during Collective Cell Migration.
Cell reports(2015 Jan)
Venero Galanternik M,Kramer KL,Piotrowski T

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ext2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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