angptl4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	angptl4
Entrez Gene ID	492647
Full Name	angiopoietin-like 4
Synonyms	fiaf,im:7144703,si:rp71-39b20.7
General protein information	Preferred Names angiopoietin-like 4 Names angiopoietin-related protein 4 angiopoietin-like 4 protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256203.1	NP_001243132.1	angiopoietin-related protein 4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANGPTL4	NP_647475.1
Pan troglodytes (chimpanzee)	ANGPTL4	XP_001147708.3
Canis lupus familiaris (dog)	ANGPTL4	XP_005633282.1
Danio rerio (zebrafish)	angptl4	NP_001243132.1
Bos taurus (cattle)	ANGPTL4	NP_001039508.1
Gallus gallus (chicken)	ANGPTL4	XP_001232284.2
Xenopus tropicalis (tropical clawed frog)	angptl4	XP_002938598.1
Rattus norvegicus (Norway rat)	Angptl4	NP_954546.1
Mus musculus (house mouse)	Angptl4	NP_065606.2

Gene Ontology
Bibliography

Related articles in PubMed

Microbiota regulate intestinal epithelial gene expression by suppressing the transcription factor Hepatocyte nuclear factor 4 alpha.
Davison JM, Lickwar CR, Song L, Breton G, Crawford GE, Rawls JF
Genome research27(7)1195-1206(2017 Jul)

Zebrafish Model for Functional Screening of Flow-Responsive Genes.
Serbanovic-Canic J, de Luca A, Warboys C, Ferreira PF, Luong LA, Hsiao S, Gauci I, Mahmoud M, Feng S, Souilhol C, Bowden N, Ashton JP, Walczak H, Firmin D, Krams R, Mason JC, Haskard DO, Sherwin S, Ridger V, Chico TJ, Evans PC
Arteriosclerosis, thrombosis, and vascular biology37(1)130-143(2017 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Intronic cis-regulatory modules mediate tissue-specific and microbial control of angptl4/fiaf transcription.
Camp JG, Jazwa AL, Trent CM, Rawls JF
PLoS genetics8(3)e1002585(2012)

Microbial colonization induces dynamic temporal and spatial patterns of NF-?B activation in the zebrafish digestive tract.
Kanther M, Sun X, M?hlbauer M, Mackey LC, Flynn EJ 3rd, Bagnat M, Jobin C, Rawls JF
Gastroenterology141(1)197-207(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that the microbiota might regulate host intestinal Angptl4 protein expression and peripheral fat storage by suppressing the activity of an intestine-specific transcriptional enhancer.
Title: Intronic cis-regulatory modules mediate tissue-specific and microbial control of angptl4/fiaf transcription.

The following angptl4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the angptl4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33194	NM_001256203.1 Latest version!	Danio rerio angiopoietin-like 4 (angptl4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

angptl4 ( NM_001256203.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001243132.1 Danio rerio angiopoietin-like 4 (angptl4), mRNA.

Price & Availability↑

CloneID	ODa33194
Clone ID Related Accession (Same CDS sequence)	NM_001256203.1
Accession Version	NM_001256203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	angiopoietin-related protein 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX530024.6. On Jan 14, 2012 this sequence version replaced XM_686956.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01002258.1, GDQQ01005546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTAC CACTGGCAAA TCTGCTGTGC ATCACAGTCC TGGCGAGCTC CGGGACGAGC 
TTCCCAATGG AGAGGAGAGG AGCTGCAGCC GGGAAAGAGA AGAGAGTGCA ATATGCAGCG 
TGGGATGATG TGAACGTGCT CGCACATGGG ATGCTGCAGC TTGGTCAGGG ACTTAAGGAG 
CATGTGGACA AGACCAAAGG CCAGGTGAGA GACATCACCA TTAAGATGAA GGTCTTTAAT 
GTCACCGTAT CCGAACTCGG CAAGCTGACT CAGCAGCTTC AAGAAGACAA TGAGCTGCTG 
AAAGCAAAAG CCCAGAATCT GGAAGACAGT GAGAGCTTGG TTCTAAATGT GTCCACGGAC 
CTGCGGCAGA AGACAGACGA GCTGCTCAAA GACAGACAGA AGGACCATGA GAGGATGAAT 
AAGCTGGAAG AAAAAGTAGA TGGCTTGATG CAAGGAGAGG GTTTGGAAGC TGCCAACAGC 
AACTACAGTG ATGCCCGAAT CATTCAGTGG ATGCTGGAGG CGCAAAATAA GCGCATTGAT 
GACCTGGTCG AGCGCATCAA GCAACAGCAG GAAAAACTGG ACAAACAGAA CATCCGCATC 
AGGACCCTGC AAAATCAGAT TACGATGAAA AACGAGCGAC TGTCTCTGAA AAGGATGGAG 
GAAGACGTAA ATCTGAACGC GAGCACTGAA CAGCGAGATT CCCCTGTTGC TTTGGCTTCT 
GACTGTCATG AGTTGTTTCT GAGGGGCGAA ACGTCAAGCG GGCTCTACAC CATCCAGCCA 
AGTGACTCCC AGCCTTTTGA AGTCTACTGC GAGATGACAC CCGAAGGAGG ATGGACAGTC 
ATCCAGAGAC GCCAGGATGG ATCTGTAGAT TTTGATCAAT TGTGGCAGGC ATATCAGAAT 
GGCTTTGGAA ACCTAAATGG TGAGTTCTGG CTCGGCCTGG AGAAGATCCA TTCTGTGTCC 
AAAGGTGGCA ACTATATCCT CAAGGTGCAG TTCTCCGATT GGCGGGATGA GATTCAAAGC 
ATCAGCTACC GATTCCATCT GAACGGCCAG GAGAACAACT ATTCCCTCCG CATCCTAGAA 
AGTCCTGCTG GAAACACAGA GAGCTCTCTG CCCACCGAAA CATCCGCAGT GCCGTTTTCC 
ACCCGCGACA AAGACAACGA CCAAAAAAAT GACCTCAACT GTGCCAAACA GTTATCAGGC 
GGTTGGTGGT TTAGCAACTG TGGTCGCTCA AACTTGAACG GCAGGTATTT CGTTACTCCT 
GCTCCGAAAC AGCGACACCA GAGAAAACAA GGAGTGTTTT GGAAAACCTG GCGTGGGCGC 
TACTATCCGC TGAAAACCAC CACCATGATG ATTGCACCCG CCGAGATTGT CAACAAATCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243132.1
CDS	93..1475
Translation	MKVPLANLLCITVLASSGTSFPMERRGAAAGKEKRVQYAAWDDVNVLAHGMLQLGQGLKEHVDKTKGQVRDITIKMKVFNVTVSELGKLTQQLQEDNELLKAKAQNLEDSESLVLNVSTDLRQKTDELLKDRQKDHERMNKLEEKVDGLMQGEGLEAANSNYSDARIIQWMLEAQNKRIDDLVERIKQQQEKLDKQNIRIRTLQNQITMKNERLSLKRMEEDVNLNASTEQRDSPVALASDCHELFLRGETSSGLYTIQPSDSQPFEVYCEMTPEGGWTVIQRRQDGSVDFDQLWQAYQNGFGNLNGEFWLGLEKIHSVSKGGNYILKVQFSDWRDEIQSISYRFHLNGQENNYSLRILESPAGNTESSLPTETSAVPFSTRDKDNDQKNDLNCAKQLSGGWWFSNCGRSNLNGRYFVTPAPKQRHQRKQGVFWKTWRGRYYPLKTTTMMIAPAEIVNKS

Target ORF information:

RefSeq Version	NM_001256203.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio angiopoietin-like 4 (angptl4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256203.1

ORF Insert Sequence:

ATGAAGGTAC CACTGGCAAA TCTGCTGTGC ATCACAGTCC TGGCGAGCTC CGGGACGAGC 
TTCCCAATGG AGAGGAGAGG AGCTGCAGCC GGGAAAGAGA AGAGAGTGCA ATATGCAGCG 
TGGGATGATG TGAACGTGCT CGCACATGGG ATGCTGCAGC TTGGTCAGGG ACTTAAGGAG 
CATGTGGACA AGACCAAAGG CCAGGTGAGA GACATCACCA TTAAGATGAA GGTCTTTAAT 
GTCACCGTAT CCGAACTCGG CAAGCTGACT CAGCAGCTTC AAGAAGACAA TGAGCTGCTG 
AAAGCAAAAG CCCAGAATCT GGAAGACAGT GAGAGCTTGG TTCTAAATGT GTCCACGGAC 
CTGCGGCAGA AGACAGACGA GCTGCTCAAA GACAGACAGA AGGACCATGA GAGGATGAAT 
AAGCTGGAAG AAAAAGTAGA TGGCTTGATG CAAGGAGAGG GTTTGGAAGC TGCCAACAGC 
AACTACAGTG ATGCCCGAAT CATTCAGTGG ATGCTGGAGG CGCAAAATAA GCGCATTGAT 
GACCTGGTCG AGCGCATCAA GCAACAGCAG GAAAAACTGG ACAAACAGAA CATCCGCATC 
AGGACCCTGC AAAATCAGAT TACGATGAAA AACGAGCGAC TGTCTCTGAA AAGGATGGAG 
GAAGACGTAA ATCTGAACGC GAGCACTGAA CAGCGAGATT CCCCTGTTGC TTTGGCTTCT 
GACTGTCATG AGTTGTTTCT GAGGGGCGAA ACGTCAAGCG GGCTCTACAC CATCCAGCCA 
AGTGACTCCC AGCCTTTTGA AGTCTACTGC GAGATGACAC CCGAAGGAGG ATGGACAGTC 
ATCCAGAGAC GCCAGGATGG ATCTGTAGAT TTTGATCAAT TGTGGCAGGC ATATCAGAAT 
GGCTTTGGAA ACCTAAATGG TGAGTTCTGG CTCGGCCTGG AGAAGATCCA TTCTGTGTCC 
AAAGGTGGCA ACTATATCCT CAAGGTGCAG TTCTCCGATT GGCGGGATGA GATTCAAAGC 
ATCAGCTACC GATTCCATCT GAACGGCCAG GAGAACAACT ATTCCCTCCG CATCCTAGAA 
AGTCCTGCTG GAAACACAGA GAGCTCTCTG CCCACCGAAA CATCCGCAGT GCCGTTTTCC 
ACCCGCGACA AAGACAACGA CCAAAAAAAT GACCTCAACT GTGCCAAACA GTTATCAGGC 
GGTTGGTGGT TTAGCAACTG TGGTCGCTCA AACTTGAACG GCAGGTATTT CGTTACTCCT 
GCTCCGAAAC AGCGACACCA GAGAAAACAA GGAGTGTTTT GGAAAACCTG GCGTGGGCGC 
TACTATCCGC TGAAAACCAC CACCATGATG ATTGCACCCG CCGAGATTGT CAACAAATCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Microbiota regulate intestinal epithelial gene expression by suppressing the transcription factor Hepatocyte nuclear factor 4 alpha.
Genome research27(7)1195-1206(2017 Jul)
Davison JM,Lickwar CR,Song L,Breton G,Crawford GE,Rawls JF

Zebrafish Model for Functional Screening of Flow-Responsive Genes.
Arteriosclerosis, thrombosis, and vascular biology37(1)130-143(2017 Jan)
Serbanovic-Canic J,de Luca A,Warboys C,Ferreira PF,Luong LA,Hsiao S,Gauci I,Mahmoud M,Feng S,Souilhol C,Bowden N,Ashton JP,Walczak H,Firmin D,Krams R,Mason JC,Haskard DO,Sherwin S,Ridger V,Chico TJ,Evans PC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Intronic cis-regulatory modules mediate tissue-specific and microbial control of angptl4/fiaf transcription.
PLoS genetics8(3)e1002585(2012)
Camp JG,Jazwa AL,Trent CM,Rawls JF

Microbial colonization induces dynamic temporal and spatial patterns of NF-?B activation in the zebrafish digestive tract.
Gastroenterology141(1)197-207(2011 Jul)
Kanther M,Sun X,M?hlbauer M,Mackey LC,Flynn EJ 3rd,Bagnat M,Jobin C,Rawls JF