ORF » Species Summary » Danio rerio » tprkb cDNA ORF clone

tprkb cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following tprkb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tprkb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa36807 NM_001007373.1
Latest version!		 Danio rerio Tp53rk binding protein (tprkb), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $104.30
$149.00
ODa141984 XM_021475009.1
Latest version!		 Danio rerio Tp53rk binding protein (tprkb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    tprkb ( NM_001007373.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007374.1 Danio rerio Tp53rk binding protein (tprkb), mRNA.
    Price & Availability↑
    CloneID ODa36807
    Clone ID Related Accession (Same CDS sequence) NM_001007373.1
    Accession Version NM_001007373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product EKC/KEOPS complex subunit TPRKB
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085466.1. ##Evidence-Data-START## Transcript exon combination :: BC085466.1, CT708733.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTATCTCA CACAGGATTT AGAACTGTTT CCTGAATACA CGGTGACTCA GCTGCTCTTC 
    AAAGATGTGA AAAATGCGAC TGAGCTCAGG AAAATGGCAG TAAATGGAGA AATAAAAGGC 
    GCCTTGATCA ATCCATCAAT GGTTGTGGAT GCATTCCAGA TCCTGGTGGC AACAAATAAA 
    GCAGTTCACC TTCATAAAAT CAGGAAAATG AAAACCAGAA GCCTTTACTC TGAAATCATT 
    TTCAATCTTT CACCAACAAA CAATATATCC GAAGCTTTCA AAAGGTTCGG AATCTCCGAC 
    AGCGACACCG CGGTTCATAT TGTGTTAGTA CACAATAAAG AAGAGACTCT TAACATTGAT 
    GACATCATCT CAAAGGTGGA TGGACAGCAA ATTGATGTTT TTCAAGTGTC CGAAATGACT 
    GATACTGCAA AAATAAAAAA GCTCTACAAA ATCACACCAC AAGAGGATAA GTGTGGCACT 
    CTTTTGGACG CTGTTGTTTG CAGGATGGCA ATTAAAGATG TGGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007374.1
    CDS88..615
    Translation

    Target ORF information:

    RefSeq Version NM_001007373.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Tp53rk binding protein (tprkb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001007373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTATCTCA CACAGGATTT AGAACTGTTT CCTGAATACA CGGTGACTCA GCTGCTCTTC 
    AAAGATGTGA AAAATGCGAC TGAGCTCAGG AAAATGGCAG TAAATGGAGA AATAAAAGGC 
    GCCTTGATCA ATCCATCAAT GGTTGTGGAT GCATTCCAGA TCCTGGTGGC AACAAATAAA 
    GCAGTTCACC TTCATAAAAT CAGGAAAATG AAAACCAGAA GCCTTTACTC TGAAATCATT 
    TTCAATCTTT CACCAACAAA CAATATATCC GAAGCTTTCA AAAGGTTCGG AATCTCCGAC 
    AGCGACACCG CGGTTCATAT TGTGTTAGTA CACAATAAAG AAGAGACTCT TAACATTGAT 
    GACATCATCT CAAAGGTGGA TGGACAGCAA ATTGATGTTT TTCAAGTGTC CGAAATGACT 
    GATACTGCAA AAATAAAAAA GCTCTACAAA ATCACACCAC AAGAGGATAA GTGTGGCACT 
    CTTTTGGACG CTGTTGTTTG CAGGATGGCA ATTAAAGATG TGGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    tprkb ( XM_021475009.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330684.1 Danio rerio Tp53rk binding protein (tprkb), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa141984
    Clone ID Related Accession (Same CDS sequence) XM_021475009.1
    Accession Version XM_021475009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product EKC/KEOPS complex subunit TPRKB isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTATCTCA CACAGGATTT AGAACTGTTT CATGAATACA CGGTGACTCA GCTGCTCTTC 
    AAAGATGTGA AAAATGCGAC TGAGCTCAGG AAAATGGCAG TAAATGGAGA AATAAAAGGC 
    GCCTTGATCA ATCCATCAAT GATCCTGGTG GCAACAAATA AAGCAGTTCA CCTTCATAAA 
    ATCGGGAAAA TGAAAACCAG AAGCCTTTAC TCTGAAATCA TTTTCAATCT TTCACCAACA 
    AACAATATAT CCGAAGCTTT CAAAAGGTTC GGAATCTCCG ACAGCGACAC CGCGGTTCAT 
    ATTGTGTTAG TACACAATAA AGAAGAGACC CTTAACATTG ATGACATCAT CTCAAAGGTG 
    GATGGACAGC AAATTGATGT TGGTCAAGTG TCCGAAATGA CTGATACTGC AAAAATAAAA 
    AAGCTCTACA AAATCACACC ACAAGAGGAT AAGTGTGGCA CTCTTTTGGA CGCTGTTGTT 
    TGCAGGATGG CAATTAAAGA TGTGGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330684.1
    CDS107..616
    Translation

    Target ORF information:

    RefSeq Version XM_021475009.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Tp53rk binding protein (tprkb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_021475009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTATCTCA CACAGGATTT AGAACTGTTT CATGAATACA CGGTGACTCA GCTGCTCTTC 
    AAAGATGTGA AAAATGCGAC TGAGCTCAGG AAAATGGCAG TAAATGGAGA AATAAAAGGC 
    GCCTTGATCA ATCCATCAAT GATCCTGGTG GCAACAAATA AAGCAGTTCA CCTTCATAAA 
    ATCGGGAAAA TGAAAACCAG AAGCCTTTAC TCTGAAATCA TTTTCAATCT TTCACCAACA 
    AACAATATAT CCGAAGCTTT CAAAAGGTTC GGAATCTCCG ACAGCGACAC CGCGGTTCAT 
    ATTGTGTTAG TACACAATAA AGAAGAGACC CTTAACATTG ATGACATCAT CTCAAAGGTG 
    GATGGACAGC AAATTGATGT TGGTCAAGTG TCCGAAATGA CTGATACTGC AAAAATAAAA 
    AAGCTCTACA AAATCACACC ACAAGAGGAT AAGTGTGGCA CTCTTTTGGA CGCTGTTGTT 
    TGCAGGATGG CAATTAAAGA TGTGGCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Acute multi-sgRNA knockdown of KEOPS complex genes reproduces the microcephaly phenotype of the stable knockout zebrafish model.
    PloS one13(1)e0191503(2018)
    Jobst-Schwan T,Schmidt JM,Schneider R,Hoogstraten CA,Ullmann JFP,Schapiro D,Majmundar AJ,Kolb A,Eddy K,Shril S,Braun DA,Poduri A,Hildebrandt F


    Mutations in KEOPS-complex genes cause nephrotic syndrome with primary microcephaly.
    Nature genetics49(10)1529-1538(2017 Oct)
    Braun DA,Rao J,Mollet G,Schapiro D,Daugeron MC,Tan W,Gribouval O,Boyer O,Revy P,Jobst-Schwan T,Schmidt JM,Lawson JA,Schanze D,Ashraf S,Ullmann JFP,Hoogstraten CA,Boddaert N,Collinet B,Martin G,Liger D,Lovric S,Furlano M,Guerrera IC,Sanchez-Ferras O,Hu JF,Boschat AC,Sanquer S,Menten B,Vergult S,De Rocker N,Airik M,Hermle T,Shril S,Widmeier E,Gee HY,Choi WI,Sadowski CE,Pabst WL,Warejko JK,Daga A,Basta T,Matejas V,Scharmann K,Kienast SD,Behnam B,Beeson B,Begtrup A,Bruce M,Ch'ng GS,Lin SP,Chang JH,Chen CH,Cho MT,Gaffney PM,Gipson PE,Hsu CH,Kari JA,Ke YY,Kiraly-Borri C,Lai WM,Lemyre E,Littlejohn RO,Masri A,Moghtaderi M,Nakamura K,Ozaltin F,Praet M,Prasad C,Prytula A,Roeder ER,Rump P,Schnur RE,Shiihara T,Sinha MD,Soliman NA,Soulami K,Sweetser DA,Tsai WH,Tsai JD,Topaloglu R,Vester U,Viskochil DH,Vatanavicharn N,Waxler JL,Wierenga KJ,Wolf MTF,Wong SN,Leidel SA,Truglio G,Dedon PC,Poduri A,Mane S,Lifton RP,Bouchard M,Kannu P,Chitayat D,Magen D,Callewaert B,van Tilbeurgh H,Zenker M,Antignac C,Hildebrandt F


    Evolution of complexity in the zebrafish synapse proteome.
    Nature communications814613(2017 Mar)
    Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG


    BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment.
    Scientific reports630330(2016 Jul)
    Boel A,Steyaert W,De Rocker N,Menten B,Callewaert B,De Paepe A,Coucke P,Willaert A


    RFX transcription factors are essential for hearing in mice.
    Nature communications68549(2015 Oct)
    Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R


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