trpa1b cDNA ORF clone, Danio rerio(zebrafish)

The following trpa1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpa1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa151682 XM_021470156.1
Latest version!
Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa37273 NM_001007066.2
Latest version!
Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa41347 XM_009297331.3
Latest version!
Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa41347 XM_009297331.2 Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa151682
    Clone ID Related Accession (Same CDS sequence) XM_021470156.1
    Accession Version XM_021470156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product transient receptor potential cation channel subfamily A member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT
    CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT
    GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT
    CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG
    ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC
    ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG
    GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG
    CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG
    CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG
    GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA
    GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA
    AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT
    TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG
    CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG
    TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA
    GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT
    GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA
    CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG
    AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC
    CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC
    CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC
    TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT
    TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG
    GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC
    TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC
    ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT
    TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG
    ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC
    TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG
    TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA
    GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG
    CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG
    AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATCTTCA CACTGCCCTT GAGGAGAAAC
    TACCTTACTG GTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325831.1
    CDS466..3423
    Translation

    Target ORF information:

    RefSeq Version XM_021470156.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT
    CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT
    GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT
    CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG
    ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC
    ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG
    GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG
    CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG
    CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG
    GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA
    GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA
    AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT
    TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG
    CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG
    TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA
    GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT
    GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA
    CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG
    AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC
    CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC
    CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC
    TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT
    TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG
    GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC
    TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC
    ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT
    TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG
    ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC
    TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG
    TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA
    GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG
    CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG
    AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATCTTCA CACTGCCCTT GAGGAGAAAC
    TACCTTACTG GTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa37273
    Clone ID Related Accession (Same CDS sequence) NM_001007066.2
    Accession Version NM_001007066.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3363bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-20
    Organism Danio rerio(zebrafish)
    Product transient receptor potential cation channel subfamily A member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY677197.1. On Oct 2, 2014 this sequence version replaced NM_001007066.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY677197.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA898404 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCTACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GCCTAATACA CTGTCATTGG ATCTCATTTG TCTTGGAAAG
    GGAGCAGAAA TTGACTCTAT TGACTGTAAG GGACTGTCTC CGCTTCTGCT CGCCTCCAGC
    TGCAGCGCTT GGAAAACAGT TGCATATCTG TTGTCCATTG GTGCAGATTT TAAAATCAAA
    GAAAAAACAG GTCGCAACTT CCTCCACTTT GTTATTCTTC AACCCAAAGG CCTGAAAAAT
    CTTCCTGAGA CGGTTTTGCA GAGCACCGCC GTGAAGGAGA TGTTGTCTGA TGAGGATGTT
    GAAGGCTGCA CTCCTCTGCA CTACGCCTGC AAGCTTGGCA TCCACGATTC GGTCAAAAAT
    ATGCTGGGTC TCAATATCTG CTTGGGTCAA AAGTCACGGG AGAAGAAATC AGCGCTTCAC
    TTCGCTGCAG AATATGGGCG TATAAATACA TGCCACCGGC TGCTGGAGAC CCTCACAGAC
    TCTAAAATGC TGAATGATTG GGATGAAAAG GGTCTGACGC CCCTCCATCT TGCTTCAAGG
    GCAGGACACG CTCAAGTAGT GGATCTGCTG CTTAGGAAGG GAGCACTGTT TCAAAGTGAT
    TACAAGGGCT GGACCTGTTT ACATCATGCA GCAGCTGAAG GATACACACA GACGATGAAA
    ATCCTGCTAG CAGCCAATGT GAAACTACTG GATGAAAAAA ATGAAGATGG GAACACAGCC
    CTTCATATAG CAGCACAAGC TGGACATGTA AGTGCTGTTT TGCTGTTATT GGACAGAGGA
    GCTGAAATCG CTCTCAACGA TGCTGACAAC TCTTTTTTGC ATGAAGCTGT GCGGAATGAG
    AGAAGAGAAG TAGTGAACGC CACCATTGAA CATGAGCGGT GTGATGAGTC AATGACGTCA
    TTTAAAGCGA AATCCCGCTG TGTTGTGCTG GACATCATAG AGTTCCTCCC AGAATCTTTT
    CAGCACCTTT TGGACCAGTG TATTACAGAA TCTGATCATG ATGCCAATAG TCAGGATTAT
    CATATCATGT ACAATTTTCA ATGGCTTCAG GCTCCCATAC AGTTGAAGAA ATATGCTAAA
    ACAGACAAAA CAAAAGCATT TCAACCACTG GCAGCATTGA ATGCCATGGT GCGGTACAAC
    CGTCTGGAGC TCCTCATTCA CCCCTTGAGC CGAAAATACC TGGAGATGAA GTGGACGGCA
    TATGGAAGCA AAGTTCATTT CCTAAACTTG GCGATATACC TGCTTGGATT GTTGCCCCTC
    ACATACCTCA TCCTCAATCT GAGACCCAGC CAGGACTTCT CCAAAGGCAA TGGCACATCT
    GTCATCATGG TGCCCGTATC TTTTAGCGAG CAACAATATT TAATCTCCGT GTGTATAATC
    ATGGTGATCG TCATGAATGT GTATTCGATT TGCAAAGAGG TGGTACAGTT GGCTCAGCAG
    CGAGTAAATT ACTTCACAGA TTTCTCAAAC CCTGCGGACT GGAGTGCAGC TATTAGCGCT
    TTGGTTTTTG TTGTACCCAT GTGCTGCAGT GTGGAGGCCA CATGGCAATG GGAAGCTGGA
    GCTTATGCTA TTCTGACTTC ATGGATTGGC TTTCTGCTTT ACTTTCAGAG ATTTGAGCGG
    ATTGGGATTT ACGTGGTGAT GTTTAATGGA ATCGTACGGA CCCTGGTGTG CATCATGGTT
    CTTTTTGTCT TCCTTTTGCT GGCTTTCGGC TTGGCTTTCT ACGCTCTGAT GCTCCACAGG
    CCGGAGTTCA GTTCCATCAG CCTAGCGTTA GCGCAAACGT TTGTGATGAC AGTAGGAGAG
    CTAAACTACC AAAGCACATT TCTTAACTCC TATGAAGAAG GCCACATGGC TTTCCCAGCC
    ATCACTTATT TCGTTTTTGT GTTTTTTGTT CTTCTCATGC CCATTCTTCT CATGAACCTA
    ATGATTGGTT TGGCTGTAGG AGACATTGCA GAGGTACAGA GAAACGCAGA GCTGAAAAGG
    ATTGCTATGC AGATAGATCT TCACACTGCC CTTGAGGAGA AACTACCTTA CTGGTTCCTG
    AAGCGAGTGG ACAAACCCTC TACCGTYGTC TATCCCAATA AATGCAAGAA AGTGCTTCTG
    GAGGCCTTGG TGCATGGTGA GATTCAGAAC ACAGTTCGAA CTCGTCTAAA CCTTTGCTCA
    CGGAAGGAAG GGCTTTTGGA GAGAGAGCTG CACAAGCAGA AGAACAGGTT GAAAGAAATG
    TCGTGCATGC TGGAAAAACA GCATAACCTG TTGAAGCTGA TTATCCAGAA GATGGAAATT
    ACATCTGAAG CTGATGAATA TGATGGTCCA CAGAATCATG GAGCTCTAAA ACAGCACACC
    TCATCCACCT CACAGAAATC AAAGTGGGTC CCGTTACTGC AAGCAATCAA GGCCAAGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007067.1
    CDS84..3446
    Translation

    Target ORF information:

    RefSeq Version NM_001007066.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001007066.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCTACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GCCTAATACA CTGTCATTGG ATCTCATTTG TCTTGGAAAG
    GGAGCAGAAA TTGACTCTAT TGACTGTAAG GGACTGTCTC CGCTTCTGCT CGCCTCCAGC
    TGCAGCGCTT GGAAAACAGT TGCATATCTG TTGTCCATTG GTGCAGATTT TAAAATCAAA
    GAAAAAACAG GTCGCAACTT CCTCCACTTT GTTATTCTTC AACCCAAAGG CCTGAAAAAT
    CTTCCTGAGA CGGTTTTGCA GAGCACCGCC GTGAAGGAGA TGTTGTCTGA TGAGGATGTT
    GAAGGCTGCA CTCCTCTGCA CTACGCCTGC AAGCTTGGCA TCCACGATTC GGTCAAAAAT
    ATGCTGGGTC TCAATATCTG CTTGGGTCAA AAGTCACGGG AGAAGAAATC AGCGCTTCAC
    TTCGCTGCAG AATATGGGCG TATAAATACA TGCCACCGGC TGCTGGAGAC CCTCACAGAC
    TCTAAAATGC TGAATGATTG GGATGAAAAG GGTCTGACGC CCCTCCATCT TGCTTCAAGG
    GCAGGACACG CTCAAGTAGT GGATCTGCTG CTTAGGAAGG GAGCACTGTT TCAAAGTGAT
    TACAAGGGCT GGACCTGTTT ACATCATGCA GCAGCTGAAG GATACACACA GACGATGAAA
    ATCCTGCTAG CAGCCAATGT GAAACTACTG GATGAAAAAA ATGAAGATGG GAACACAGCC
    CTTCATATAG CAGCACAAGC TGGACATGTA AGTGCTGTTT TGCTGTTATT GGACAGAGGA
    GCTGAAATCG CTCTCAACGA TGCTGACAAC TCTTTTTTGC ATGAAGCTGT GCGGAATGAG
    AGAAGAGAAG TAGTGAACGC CACCATTGAA CATGAGCGGT GTGATGAGTC AATGACGTCA
    TTTAAAGCGA AATCCCGCTG TGTTGTGCTG GACATCATAG AGTTCCTCCC AGAATCTTTT
    CAGCACCTTT TGGACCAGTG TATTACAGAA TCTGATCATG ATGCCAATAG TCAGGATTAT
    CATATCATGT ACAATTTTCA ATGGCTTCAG GCTCCCATAC AGTTGAAGAA ATATGCTAAA
    ACAGACAAAA CAAAAGCATT TCAACCACTG GCAGCATTGA ATGCCATGGT GCGGTACAAC
    CGTCTGGAGC TCCTCATTCA CCCCTTGAGC CGAAAATACC TGGAGATGAA GTGGACGGCA
    TATGGAAGCA AAGTTCATTT CCTAAACTTG GCGATATACC TGCTTGGATT GTTGCCCCTC
    ACATACCTCA TCCTCAATCT GAGACCCAGC CAGGACTTCT CCAAAGGCAA TGGCACATCT
    GTCATCATGG TGCCCGTATC TTTTAGCGAG CAACAATATT TAATCTCCGT GTGTATAATC
    ATGGTGATCG TCATGAATGT GTATTCGATT TGCAAAGAGG TGGTACAGTT GGCTCAGCAG
    CGAGTAAATT ACTTCACAGA TTTCTCAAAC CCTGCGGACT GGAGTGCAGC TATTAGCGCT
    TTGGTTTTTG TTGTACCCAT GTGCTGCAGT GTGGAGGCCA CATGGCAATG GGAAGCTGGA
    GCTTATGCTA TTCTGACTTC ATGGATTGGC TTTCTGCTTT ACTTTCAGAG ATTTGAGCGG
    ATTGGGATTT ACGTGGTGAT GTTTAATGGA ATCGTACGGA CCCTGGTGTG CATCATGGTT
    CTTTTTGTCT TCCTTTTGCT GGCTTTCGGC TTGGCTTTCT ACGCTCTGAT GCTCCACAGG
    CCGGAGTTCA GTTCCATCAG CCTAGCGTTA GCGCAAACGT TTGTGATGAC AGTAGGAGAG
    CTAAACTACC AAAGCACATT TCTTAACTCC TATGAAGAAG GCCACATGGC TTTCCCAGCC
    ATCACTTATT TCGTTTTTGT GTTTTTTGTT CTTCTCATGC CCATTCTTCT CATGAACCTA
    ATGATTGGTT TGGCTGTAGG AGACATTGCA GAGGTACAGA GAAACGCAGA GCTGAAAAGG
    ATTGCTATGC AGATAGATCT TCACACTGCC CTTGAGGAGA AACTACCTTA CTGGTTCCTG
    AAGCGAGTGG ACAAACCCTC TACCGTYGTC TATCCCAATA AATGCAAGAA AGTGCTTCTG
    GAGGCCTTGG TGCATGGTGA GATTCAGAAC ACAGTTCGAA CTCGTCTAAA CCTTTGCTCA
    CGGAAGGAAG GGCTTTTGGA GAGAGAGCTG CACAAGCAGA AGAACAGGTT GAAAGAAATG
    TCGTGCATGC TGGAAAAACA GCATAACCTG TTGAAGCTGA TTATCCAGAA GATGGAAATT
    ACATCTGAAG CTGATGAATA TGATGGTCCA CAGAATCATG GAGCTCTAAA ACAGCACACC
    TCATCCACCT CACAGAAATC AAAGTGGGTC CCGTTACTGC AAGCAATCAA GGCCAAGAAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41347
    Clone ID Related Accession (Same CDS sequence) XM_009297331.2 , XM_009297331.3
    Accession Version XM_009297331.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product transient receptor potential cation channel subfamily A member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT
    CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT
    GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT
    CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG
    ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC
    ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG
    GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG
    CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG
    CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG
    GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA
    GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA
    AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT
    TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG
    CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG
    TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA
    GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT
    GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA
    CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG
    AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC
    CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC
    CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC
    TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT
    TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG
    GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC
    TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC
    ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT
    TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG
    ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC
    TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG
    TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA
    GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG
    CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG
    AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG
    AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT
    AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA
    ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG
    AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG
    ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT
    GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG
    CAAGCAATCA AGGCCAAGAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295606.1
    CDS111..3434
    Translation

    Target ORF information:

    RefSeq Version XM_009297331.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297331.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT
    CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT
    GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT
    CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG
    ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC
    ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG
    GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG
    CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG
    CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG
    GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA
    GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA
    AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT
    TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG
    CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG
    TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA
    GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT
    GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA
    CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG
    AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC
    CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC
    CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC
    TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT
    TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG
    GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC
    TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC
    ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT
    TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG
    ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC
    TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG
    TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA
    GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG
    CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG
    AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG
    AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT
    AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA
    ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG
    AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG
    ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT
    GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG
    CAAGCAATCA AGGCCAAGAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41347
    Clone ID Related Accession (Same CDS sequence) XM_009297331.2 , XM_009297331.3
    Accession Version XM_009297331.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3324bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product transient receptor potential cation channel subfamily A member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT
    CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT
    GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT
    CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG
    ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC
    ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG
    GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG
    CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG
    CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG
    GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA
    GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA
    AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT
    TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG
    CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG
    TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA
    GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT
    GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA
    CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG
    AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC
    CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC
    CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC
    TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT
    TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG
    GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC
    TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC
    ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT
    TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG
    ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC
    TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG
    TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA
    GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG
    CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG
    AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG
    AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT
    AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA
    ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG
    AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG
    ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT
    GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG
    CAAGCAATCA AGGCCAAGAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009295606.1
    CDS449..3772
    Translation

    Target ORF information:

    RefSeq Version XM_009297331.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297331.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
    GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC
    ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC
    AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC
    ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG
    GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC
    AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT
    CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG
    GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG
    ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT
    GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT
    GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT
    CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA
    GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT
    CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT
    CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG
    GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT
    CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT
    GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT
    CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG
    ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC
    ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG
    GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG
    CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG
    CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG
    GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA
    GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA
    AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT
    TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG
    CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG
    TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA
    GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT
    GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA
    CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG
    AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC
    CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC
    CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC
    TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT
    TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG
    GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC
    TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC
    ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT
    TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG
    ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC
    TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG
    TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA
    GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG
    CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG
    AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG
    AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT
    AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA
    ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG
    AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG
    ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT
    GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG
    CAAGCAATCA AGGCCAAGAA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.