trpa1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trpa1b
Entrez Gene ID	474353
Full Name	transient receptor potential cation channel, subfamily A, member 1b
Synonyms	trpa1l,trpa2
General protein information	Preferred Names transient receptor potential cation channel, subfamily A, member 1b Names transient receptor potential cation channel subfamily A member 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021470156.1	XP_021325831.1	transient receptor potential cation channel subfamily A member 1 isoform X2
NM_001007066.2	NP_001007067.1	transient receptor potential cation channel subfamily A member 1
XM_009297331.2	XP_009295606.1	transient receptor potential cation channel subfamily A member 1 isoform X1
XM_009297331.3	XP_009295606.1	transient receptor potential cation channel subfamily A member 1 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TRPA1	XP_002692772.2
Canis lupus familiaris (dog)	TRPA1	XP_544123.2
Caenorhabditis elegans (roundworm)	trpa-1	NP_502249.3
Pan troglodytes (chimpanzee)	TRPA1	XP_519806.2
Mus musculus (house mouse)	Trpa1	NP_808449.1
Danio rerio (zebrafish)	trpa1a	NP_001007066.1
Danio rerio (zebrafish)	trpa1b	NP_001007067.1
Drosophila melanogaster (fruit fly)	TrpA1	NP_648263.5
Homo sapiens (human)	TRPA1	NP_015628.2
	TRPA1	XP_001083172.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004863	XP_314325.4
Rattus norvegicus (Norway rat)	Trpa1	NP_997491.1
Gallus gallus (chicken)	TRPA1	XP_418294.4
Xenopus tropicalis (tropical clawed frog)	trpa1	NP_001121434.1

Gene Ontology
Bibliography

Related articles in PubMed

Chemical and thermal sensitivity of medaka TRPA1 analyzed in heterologous expression system.
Oda M, Saito K, Hatta S, Kubo Y, Saitoh O
Biochemical and biophysical research communications494(1-2)194-201(2017 12)

A high-conductance chemo-optogenetic system based on the vertebrate channel Trpa1b.
Lam PY, Mendu SK, Mills RW, Zheng B, Padilla H, Milan DJ, Desai BN, Peterson RT
Scientific reports7(1)11839(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High-throughput behavioral analyses of trpa1a and trpa1b mutant larvae indicate that TRPA1b is necessary for behavioral responses to chemical irritants.
Title: Zebrafish TRPA1 channels are required for chemosensation but not for thermosensation or mechanosensory hair cell function.

The following trpa1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpa1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151682	XM_021470156.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa37273	NM_001007066.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa41347	XM_009297331.3 Latest version!	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa41347	XM_009297331.2	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpa1b ( XM_021470156.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325831.1 Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa151682
Clone ID Related Accession (Same CDS sequence)	XM_021470156.1
Accession Version	XM_021470156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily A member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT 
CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT 
GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT 
CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG 
ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC 
ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG 
GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG 
CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG 
CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG 
GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA 
GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA 
AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT 
TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG 
CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG 
TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA 
GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT 
GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA 
CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG 
AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC 
CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC 
CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC 
TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT 
TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG 
GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC 
TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC 
ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT 
TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG 
ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC 
TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG 
TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA 
GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG 
CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG 
AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATCTTCA CACTGCCCTT GAGGAGAAAC 
TACCTTACTG GTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325831.1
CDS	466..3423
Translation	MQFGKELVRRNSFYKCVINEDETEESADVFELAFKGKASAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGPEVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLHFVILQPKGLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVKNMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGALFQSDYKGWTCLHHAAAEGYTQTMKILLAANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGSKVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYLISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYLVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIFTLPLRRNYLTGS

Target ORF information:

RefSeq Version	XM_021470156.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470156.1

ORF Insert Sequence:

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT 
CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT 
GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT 
CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG 
ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC 
ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG 
GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG 
CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG 
CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG 
GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA 
GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA 
AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT 
TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG 
CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG 
TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA 
GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT 
GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA 
CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG 
AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC 
CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC 
CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC 
TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT 
TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG 
GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC 
TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC 
ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT 
TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG 
ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC 
TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG 
TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA 
GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG 
CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG 
AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATCTTCA CACTGCCCTT GAGGAGAAAC 
TACCTTACTG GTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpa1b ( NM_001007066.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001007067.1 Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), mRNA.

Price & Availability↑

CloneID	ODa37273
Clone ID Related Accession (Same CDS sequence)	NM_001007066.2
Accession Version	NM_001007066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-20
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily A member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY677197.1. On Oct 2, 2014 this sequence version replaced NM_001007066.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY677197.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168447, SAMEA898404 [ECO:0000350] ##Evidence-Data-END##

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCTACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GCCTAATACA CTGTCATTGG ATCTCATTTG TCTTGGAAAG 
GGAGCAGAAA TTGACTCTAT TGACTGTAAG GGACTGTCTC CGCTTCTGCT CGCCTCCAGC 
TGCAGCGCTT GGAAAACAGT TGCATATCTG TTGTCCATTG GTGCAGATTT TAAAATCAAA 
GAAAAAACAG GTCGCAACTT CCTCCACTTT GTTATTCTTC AACCCAAAGG CCTGAAAAAT 
CTTCCTGAGA CGGTTTTGCA GAGCACCGCC GTGAAGGAGA TGTTGTCTGA TGAGGATGTT 
GAAGGCTGCA CTCCTCTGCA CTACGCCTGC AAGCTTGGCA TCCACGATTC GGTCAAAAAT 
ATGCTGGGTC TCAATATCTG CTTGGGTCAA AAGTCACGGG AGAAGAAATC AGCGCTTCAC 
TTCGCTGCAG AATATGGGCG TATAAATACA TGCCACCGGC TGCTGGAGAC CCTCACAGAC 
TCTAAAATGC TGAATGATTG GGATGAAAAG GGTCTGACGC CCCTCCATCT TGCTTCAAGG 
GCAGGACACG CTCAAGTAGT GGATCTGCTG CTTAGGAAGG GAGCACTGTT TCAAAGTGAT 
TACAAGGGCT GGACCTGTTT ACATCATGCA GCAGCTGAAG GATACACACA GACGATGAAA 
ATCCTGCTAG CAGCCAATGT GAAACTACTG GATGAAAAAA ATGAAGATGG GAACACAGCC 
CTTCATATAG CAGCACAAGC TGGACATGTA AGTGCTGTTT TGCTGTTATT GGACAGAGGA 
GCTGAAATCG CTCTCAACGA TGCTGACAAC TCTTTTTTGC ATGAAGCTGT GCGGAATGAG 
AGAAGAGAAG TAGTGAACGC CACCATTGAA CATGAGCGGT GTGATGAGTC AATGACGTCA 
TTTAAAGCGA AATCCCGCTG TGTTGTGCTG GACATCATAG AGTTCCTCCC AGAATCTTTT 
CAGCACCTTT TGGACCAGTG TATTACAGAA TCTGATCATG ATGCCAATAG TCAGGATTAT 
CATATCATGT ACAATTTTCA ATGGCTTCAG GCTCCCATAC AGTTGAAGAA ATATGCTAAA 
ACAGACAAAA CAAAAGCATT TCAACCACTG GCAGCATTGA ATGCCATGGT GCGGTACAAC 
CGTCTGGAGC TCCTCATTCA CCCCTTGAGC CGAAAATACC TGGAGATGAA GTGGACGGCA 
TATGGAAGCA AAGTTCATTT CCTAAACTTG GCGATATACC TGCTTGGATT GTTGCCCCTC 
ACATACCTCA TCCTCAATCT GAGACCCAGC CAGGACTTCT CCAAAGGCAA TGGCACATCT 
GTCATCATGG TGCCCGTATC TTTTAGCGAG CAACAATATT TAATCTCCGT GTGTATAATC 
ATGGTGATCG TCATGAATGT GTATTCGATT TGCAAAGAGG TGGTACAGTT GGCTCAGCAG 
CGAGTAAATT ACTTCACAGA TTTCTCAAAC CCTGCGGACT GGAGTGCAGC TATTAGCGCT 
TTGGTTTTTG TTGTACCCAT GTGCTGCAGT GTGGAGGCCA CATGGCAATG GGAAGCTGGA 
GCTTATGCTA TTCTGACTTC ATGGATTGGC TTTCTGCTTT ACTTTCAGAG ATTTGAGCGG 
ATTGGGATTT ACGTGGTGAT GTTTAATGGA ATCGTACGGA CCCTGGTGTG CATCATGGTT 
CTTTTTGTCT TCCTTTTGCT GGCTTTCGGC TTGGCTTTCT ACGCTCTGAT GCTCCACAGG 
CCGGAGTTCA GTTCCATCAG CCTAGCGTTA GCGCAAACGT TTGTGATGAC AGTAGGAGAG 
CTAAACTACC AAAGCACATT TCTTAACTCC TATGAAGAAG GCCACATGGC TTTCCCAGCC 
ATCACTTATT TCGTTTTTGT GTTTTTTGTT CTTCTCATGC CCATTCTTCT CATGAACCTA 
ATGATTGGTT TGGCTGTAGG AGACATTGCA GAGGTACAGA GAAACGCAGA GCTGAAAAGG 
ATTGCTATGC AGATAGATCT TCACACTGCC CTTGAGGAGA AACTACCTTA CTGGTTCCTG 
AAGCGAGTGG ACAAACCCTC TACCGTYGTC TATCCCAATA AATGCAAGAA AGTGCTTCTG 
GAGGCCTTGG TGCATGGTGA GATTCAGAAC ACAGTTCGAA CTCGTCTAAA CCTTTGCTCA 
CGGAAGGAAG GGCTTTTGGA GAGAGAGCTG CACAAGCAGA AGAACAGGTT GAAAGAAATG 
TCGTGCATGC TGGAAAAACA GCATAACCTG TTGAAGCTGA TTATCCAGAA GATGGAAATT 
ACATCTGAAG CTGATGAATA TGATGGTCCA CAGAATCATG GAGCTCTAAA ACAGCACACC 
TCATCCACCT CACAGAAATC AAAGTGGGTC CCGTTACTGC AAGCAATCAA GGCCAAGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007067.1
CDS	84..3446
Translation	MQFGKELVRRNSFYKCVINEDETEESADVFELAFKGKASAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGPEVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKPNTLSLDLICLGKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLHFVILQPKGLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVKNMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGALFQSDYKGWTCLHHAAAEGYTQTMKILLAANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGSKVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYLISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYFVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIDLHTALEEKLPYWFLKRVDKPSTnullVYPNKCKKVLLEALVHGEIQNTVRTRLNLCSRKEGLLERELHKQKNRLKEMSCMLEKQHNLLKLIIQKMEITSEADEYDGPQNHGALKQHTSSTSQKSKWVPLLQAIKAKK

Target ORF information:

RefSeq Version	NM_001007066.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007066.2

ORF Insert Sequence:

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCTACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GCCTAATACA CTGTCATTGG ATCTCATTTG TCTTGGAAAG 
GGAGCAGAAA TTGACTCTAT TGACTGTAAG GGACTGTCTC CGCTTCTGCT CGCCTCCAGC 
TGCAGCGCTT GGAAAACAGT TGCATATCTG TTGTCCATTG GTGCAGATTT TAAAATCAAA 
GAAAAAACAG GTCGCAACTT CCTCCACTTT GTTATTCTTC AACCCAAAGG CCTGAAAAAT 
CTTCCTGAGA CGGTTTTGCA GAGCACCGCC GTGAAGGAGA TGTTGTCTGA TGAGGATGTT 
GAAGGCTGCA CTCCTCTGCA CTACGCCTGC AAGCTTGGCA TCCACGATTC GGTCAAAAAT 
ATGCTGGGTC TCAATATCTG CTTGGGTCAA AAGTCACGGG AGAAGAAATC AGCGCTTCAC 
TTCGCTGCAG AATATGGGCG TATAAATACA TGCCACCGGC TGCTGGAGAC CCTCACAGAC 
TCTAAAATGC TGAATGATTG GGATGAAAAG GGTCTGACGC CCCTCCATCT TGCTTCAAGG 
GCAGGACACG CTCAAGTAGT GGATCTGCTG CTTAGGAAGG GAGCACTGTT TCAAAGTGAT 
TACAAGGGCT GGACCTGTTT ACATCATGCA GCAGCTGAAG GATACACACA GACGATGAAA 
ATCCTGCTAG CAGCCAATGT GAAACTACTG GATGAAAAAA ATGAAGATGG GAACACAGCC 
CTTCATATAG CAGCACAAGC TGGACATGTA AGTGCTGTTT TGCTGTTATT GGACAGAGGA 
GCTGAAATCG CTCTCAACGA TGCTGACAAC TCTTTTTTGC ATGAAGCTGT GCGGAATGAG 
AGAAGAGAAG TAGTGAACGC CACCATTGAA CATGAGCGGT GTGATGAGTC AATGACGTCA 
TTTAAAGCGA AATCCCGCTG TGTTGTGCTG GACATCATAG AGTTCCTCCC AGAATCTTTT 
CAGCACCTTT TGGACCAGTG TATTACAGAA TCTGATCATG ATGCCAATAG TCAGGATTAT 
CATATCATGT ACAATTTTCA ATGGCTTCAG GCTCCCATAC AGTTGAAGAA ATATGCTAAA 
ACAGACAAAA CAAAAGCATT TCAACCACTG GCAGCATTGA ATGCCATGGT GCGGTACAAC 
CGTCTGGAGC TCCTCATTCA CCCCTTGAGC CGAAAATACC TGGAGATGAA GTGGACGGCA 
TATGGAAGCA AAGTTCATTT CCTAAACTTG GCGATATACC TGCTTGGATT GTTGCCCCTC 
ACATACCTCA TCCTCAATCT GAGACCCAGC CAGGACTTCT CCAAAGGCAA TGGCACATCT 
GTCATCATGG TGCCCGTATC TTTTAGCGAG CAACAATATT TAATCTCCGT GTGTATAATC 
ATGGTGATCG TCATGAATGT GTATTCGATT TGCAAAGAGG TGGTACAGTT GGCTCAGCAG 
CGAGTAAATT ACTTCACAGA TTTCTCAAAC CCTGCGGACT GGAGTGCAGC TATTAGCGCT 
TTGGTTTTTG TTGTACCCAT GTGCTGCAGT GTGGAGGCCA CATGGCAATG GGAAGCTGGA 
GCTTATGCTA TTCTGACTTC ATGGATTGGC TTTCTGCTTT ACTTTCAGAG ATTTGAGCGG 
ATTGGGATTT ACGTGGTGAT GTTTAATGGA ATCGTACGGA CCCTGGTGTG CATCATGGTT 
CTTTTTGTCT TCCTTTTGCT GGCTTTCGGC TTGGCTTTCT ACGCTCTGAT GCTCCACAGG 
CCGGAGTTCA GTTCCATCAG CCTAGCGTTA GCGCAAACGT TTGTGATGAC AGTAGGAGAG 
CTAAACTACC AAAGCACATT TCTTAACTCC TATGAAGAAG GCCACATGGC TTTCCCAGCC 
ATCACTTATT TCGTTTTTGT GTTTTTTGTT CTTCTCATGC CCATTCTTCT CATGAACCTA 
ATGATTGGTT TGGCTGTAGG AGACATTGCA GAGGTACAGA GAAACGCAGA GCTGAAAAGG 
ATTGCTATGC AGATAGATCT TCACACTGCC CTTGAGGAGA AACTACCTTA CTGGTTCCTG 
AAGCGAGTGG ACAAACCCTC TACCGTYGTC TATCCCAATA AATGCAAGAA AGTGCTTCTG 
GAGGCCTTGG TGCATGGTGA GATTCAGAAC ACAGTTCGAA CTCGTCTAAA CCTTTGCTCA 
CGGAAGGAAG GGCTTTTGGA GAGAGAGCTG CACAAGCAGA AGAACAGGTT GAAAGAAATG 
TCGTGCATGC TGGAAAAACA GCATAACCTG TTGAAGCTGA TTATCCAGAA GATGGAAATT 
ACATCTGAAG CTGATGAATA TGATGGTCCA CAGAATCATG GAGCTCTAAA ACAGCACACC 
TCATCCACCT CACAGAAATC AAAGTGGGTC CCGTTACTGC AAGCAATCAA GGCCAAGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpa1b ( XM_009297331.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295606.1 Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41347
Clone ID Related Accession (Same CDS sequence)	XM_009297331.2 , XM_009297331.3
Accession Version	XM_009297331.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily A member 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297331.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT 
CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT 
GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT 
CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG 
ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC 
ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG 
GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG 
CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG 
CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG 
GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA 
GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA 
AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT 
TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG 
CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG 
TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA 
GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT 
GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA 
CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG 
AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC 
CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC 
CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC 
TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT 
TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG 
GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC 
TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC 
ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT 
TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG 
ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC 
TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG 
TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA 
GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG 
CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG 
AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG 
AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT 
AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA 
ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG 
AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG 
ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT 
GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG 
CAAGCAATCA AGGCCAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295606.1
CDS	111..3434
Translation	MQFGKELVRRNSFYKCVINEDETEESADVFELAFKGKASAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGPEVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLHFVILQPKGLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVKNMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGALFQSDYKGWTCLHHAAAEGYTQTMKILLAANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGSKVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYLISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYLVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIDLHTALEEKLPYWFLKRVDKPSTVVYPNKCKKVLLEALVHGEIQNTVRTRLNLCSRKEGLLERELHKQKNRLKEMSCMLEKQHNLLKLIIQKMEITSEADEYDGPQNHGALKQHTSSTSQKSKWVPLLQAIKAKK

Target ORF information:

RefSeq Version	XM_009297331.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297331.2

ORF Insert Sequence:

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT 
CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT 
GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT 
CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG 
ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC 
ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG 
GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG 
CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG 
CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG 
GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA 
GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA 
AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT 
TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG 
CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG 
TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA 
GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT 
GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA 
CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG 
AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC 
CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC 
CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC 
TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT 
TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG 
GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC 
TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC 
ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT 
TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG 
ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC 
TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG 
TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA 
GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG 
CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG 
AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG 
AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT 
AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA 
ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG 
AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG 
ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT 
GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG 
CAAGCAATCA AGGCCAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpa1b ( XM_009297331.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295606.1 Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41347
Clone ID Related Accession (Same CDS sequence)	XM_009297331.2 , XM_009297331.3
Accession Version	XM_009297331.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily A member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297331.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT 
CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT 
GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT 
CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG 
ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC 
ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG 
GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG 
CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG 
CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG 
GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA 
GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA 
AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT 
TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG 
CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG 
TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA 
GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT 
GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA 
CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG 
AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC 
CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC 
CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC 
TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT 
TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG 
GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC 
TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC 
ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT 
TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG 
ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC 
TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG 
TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA 
GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG 
CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG 
AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG 
AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT 
AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA 
ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG 
AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG 
ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT 
GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG 
CAAGCAATCA AGGCCAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295606.1
CDS	449..3772
Translation	MQFGKELVRRNSFYKCVINEDETEESADVFELAFKGKASAIDRLIQKGPEHLSLRDENGASLLHYASAGGNLDIIRLIVSIVGPEVLNVQDEQGRTPLHWAVEQDQQQSCAVLLDLGADPNILNNALMGPLHLAVTKQYNHLAEVLLSCDKTNSNLEGDLGNTPVMLACSNNNCQALQILIKRGAKMCIQNKLGHYPIHTVAFAGAKEAMEMVLKIGEELGVSSTLHINYLDKSKSTPLHLAVRGGNIEVIKLCILKGAKVEQHQSGKCTALHFACSQGSLEAVKIMLSSYNRTEDIVNIRDGANRTPLHRATLFDHVELAEYLISKGAEIDSIDCKGLSPLLLASSCSAWKTVAYLLSIGADFKIKEKTGRNFLHFVILQPKGLKNLPETVLQSTAVKEMLSDEDVEGCTPLHYACKLGIHDSVKNMLGLNICLGQKSREKKSALHFAAEYGRINTCHRLLETLTDSKMLNDWDEKGLTPLHLASRAGHAQVVDLLLRKGALFQSDYKGWTCLHHAAAEGYTQTMKILLAANVKLLDEKNEDGNTALHIAAQAGHVSAVLLLLDRGAEIALNDADNSFLHEAVRNERREVVNATIEHERCDESMTSFKAKSRCVVLDIIEFLPESFQHLLDQCITESDHDANSQDYHIMYNFQWLQAPIQLKKYAKTDKTKAFQPLAALNAMVRYNRLELLIHPLSRKYLEMKWTAYGSKVHFLNLAIYLLGLLPLTYLILNLRPSQDFSKGNGTSVIMVPVSFSEQQYLISVCIIMVIVMNVYSICKEVVQLAQQRVNYFTDFSNPADWSAAISALVFVVPMCCSVEATWQWEAGAYAILTSWIGFLLYFQRFERIGIYVVMFNGIVRTLVCIMVLFVFLLLAFGLAFYALMLHRPEFSSISLALAQTFVMTVGELNYQSTFLNSYEEGHMAFPAITYLVFVFFVLLMPILLMNLMIGLAVGDIAEVQRNAELKRIAMQIDLHTALEEKLPYWFLKRVDKPSTVVYPNKCKKVLLEALVHGEIQNTVRTRLNLCSRKEGLLERELHKQKNRLKEMSCMLEKQHNLLKLIIQKMEITSEADEYDGPQNHGALKQHTSSTSQKSKWVPLLQAIKAKK

Target ORF information:

RefSeq Version	XM_009297331.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily A, member 1b (trpa1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297331.3

ORF Insert Sequence:

ATGCAGTTTG GAAAGGAGTT AGTGAGGCGA AACAGCTTCT ACAAATGTGT GATAAACGAG 
GACGAAACGG AGGAGTCTGC AGATGTCTTT GAGTTGGCTT TCAAAGGAAA GGCTTCTGCC 
ATCGATCGTC TGATCCAGAA AGGTCCAGAA CACCTAAGCC TCAGGGATGA GAATGGAGCC 
AGTCTGCTGC ACTATGCTTC AGCTGGAGGA AACCTGGATA TCATTCGGCT CATTGTTTCC 
ATTGTGGGCC CTGAGGTGCT GAATGTGCAG GATGAGCAGG GCCGAACTCC TCTGCACTGG 
GCTGTGGAGC AGGATCAACA ACAGAGCTGC GCTGTACTGC TGGATCTGGG TGCTGACCCC 
AACATCCTCA ACAATGCCCT GATGGGCCCT CTGCACCTGG CTGTCACCAA ACAATACAAT 
CATCTAGCAG AGGTCCTTCT GTCTTGTGAC AAAACCAATA GCAATCTGGA GGGGGATCTG 
GGAAACACCC CTGTCATGCT GGCCTGCTCC AATAACAACT GCCAAGCTCT CCAGATCCTG 
ATCAAGCGCG GTGCCAAGAT GTGTATTCAG AACAAGCTGG GTCACTATCC CATTCATACT 
GTTGCTTTCG CAGGTGCCAA AGAGGCCATG GAGATGGTAT TGAAAATTGG AGAAGAACTT 
GGTGTATCAT CTACATTGCA CATTAATTAT TTGGATAAGT CCAAAAGCAC ACCTCTTCAT 
CTGGCTGTAC GCGGAGGCAA TATCGAGGTG ATCAAACTCT GCATCTTGAA AGGAGCCAAA 
GTAGAACAAC ATCAGAGTGG CAAATGCACG GCCCTCCACT TTGCCTGCAG TCAGGGTTCT 
CTGGAGGCTG TCAAAATCAT GCTTTCCTCT TACAACAGAA CAGAAGATAT CGTCAACATT 
CGGGATGGAG CCAATCGGAC ACCTTTGCAT AGAGCCACGC TGTTTGATCA CGTTGAGTTG 
GCTGAATATC TTATTTCAAA GGGAGCAGAA ATTGACTCTA TTGACTGTAA GGGACTGTCT 
CCGCTTCTGC TCGCCTCCAG CTGCAGCGCT TGGAAAACAG TTGCATATCT GTTGTCCATT 
GGTGCAGATT TTAAAATCAA AGAAAAAACA GGTCGCAACT TCCTCCACTT TGTTATTCTT 
CAACCTAAAG GCCTAAAAAA TCTTCCTGAG ACGGTTTTGC AGAGCACCGC CGTGAAGGAG 
ATGTTGTCTG ATGAGGATGT TGAAGGCTGC ACTCCTCTGC ACTACGCCTG CAAGCTTGGC 
ATCCACGATT CGGTCAAAAA TATGCTGGGT CTCAATATCT GCTTGGGTCA AAAGTCACGG 
GAGAAGAAAT CAGCGCTTCA CTTCGCTGCA GAATATGGGC GTATAAATAC ATGCCACCGG 
CTGCTGGAGA CCCTCACAGA CTCTAAAATG CTGAATGATT GGGATGAAAA GGGTCTGACG 
CCCCTCCATC TTGCTTCAAG GGCAGGACAC GCTCAAGTAG TGGATCTGCT GCTTAGGAAG 
GGAGCACTGT TTCAAAGTGA TTACAAGGGC TGGACCTGTT TACATCATGC AGCAGCTGAA 
GGATACACAC AGACGATGAA AATCCTGCTG GCAGCCAATG TGAAACTACT AGATGAAAAA 
AATGAAGATG GGAACACAGC CCTTCATATA GCAGCACAAG CTGGACATGT AAGTGCTGTT 
TTGCTGTTAT TGGACAGAGG AGCTGAAATC GCTCTCAACG ATGCTGACAA CTCTTTTTTG 
CATGAAGCTG TGCGGAATGA GAGAAGAGAA GTAGTGAACG CCACCATTGA ACATGAGCGG 
TGTGATGAGT CAATGACGTC ATTTAAAGCG AAATCCCGCT GTGTTGTGCT GGACATCATA 
GAGTTCCTCC CAGAATCTTT TCAGCACCTT TTGGACCAGT GTATTACAGA ATCTGATCAT 
GATGCCAATA GTCAGGATTA TCATATCATG TACAATTTTC AATGGCTTCA GGCTCCCATA 
CAGTTGAAGA AATATGCTAA AACAGACAAA ACAAAAGCAT TTCAACCACT GGCAGCATTG 
AATGCCATGG TGCGGTACAA CCGTCTGGAG CTCCTCATTC ACCCCTTGAG CCGAAAATAC 
CTGGAGATGA AGTGGACGGC ATATGGAAGC AAAGTTCATT TCCTAAACTT GGCGATATAC 
CTGCTTGGAT TGTTGCCCCT CACATACCTC ATCCTCAATC TGAGACCCAG CCAGGACTTC 
TCCAAAGGCA ATGGCACATC TGTCATCATG GTGCCCGTAT CTTTTAGCGA GCAACAATAT 
TTAATCTCCG TGTGTATAAT CATGGTGATC GTCATGAATG TGTATTCGAT TTGCAAAGAG 
GTGGTACAGT TGGCTCAGCA GCGAGTAAAT TACTTCACAG ATTTCTCAAA CCCTGCGGAC 
TGGAGTGCAG CTATTAGCGC TTTGGTTTTT GTTGTACCCA TGTGCTGCAG TGTGGAGGCC 
ACATGGCAAT GGGAAGCTGG AGCTTATGCT ATTCTGACTT CATGGATTGG CTTTCTGCTT 
TACTTTCAGA GATTTGAGCG GATTGGGATT TACGTGGTGA TGTTTAATGG AATCGTACGG 
ACCCTGGTGT GCATCATGGT TCTTTTTGTC TTCCTTTTGC TGGCTTTCGG CTTGGCTTTC 
TACGCTCTGA TGCTCCACAG GCCGGAGTTC AGTTCCATCA GCCTAGCGTT AGCGCAAACG 
TTTGTGATGA CAGTAGGAGA GCTAAACTAC CAAAGCACAT TTCTTAACTC CTATGAAGAA 
GGCCACATGG CTTTCCCAGC CATCACTTAT TTAGTTTTTG TGTTTTTTGT TCTTCTCATG 
CCCATTCTTC TCATGAACCT AATGATTGGT TTGGCTGTAG GAGACATTGC AGAGGTACAG 
AGAAACGCAG AGCTGAAAAG GATTGCTATG CAGATAGATC TTCACACTGC CCTTGAGGAG 
AAACTACCTT ACTGGTTCCT GAAGCGAGTG GACAAACCCT CTACCGTTGT CTATCCCAAT 
AAATGCAAGA AAGTGCTTCT GGAGGCCTTG GTGCATGGTG AGATTCAGAA CACAGTTCGA 
ACTCGTCTAA ACCTTTGCTC ACGGAAGGAA GGGCTTTTGG AGAGAGAGCT GCACAAGCAG 
AAGAACAGGT TGAAAGAAAT GTCGTGCATG CTGGAAAAAC AGCATAACCT GTTGAAGCTG 
ATTATCCAGA AGATGGAAAT TACATCTGAA GCTGATGAAT ATGATGGTCC ACAGAATCAT 
GGAGCTCTAA AACAGCACAC CTCATCCACC TCACAGAAAT CAAAGTGGGT CCCGTTACTG 
CAAGCAATCA AGGCCAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chemical and thermal sensitivity of medaka TRPA1 analyzed in heterologous expression system.
Biochemical and biophysical research communications494(1-2)194-201(2017 12)
Oda M,Saito K,Hatta S,Kubo Y,Saitoh O

A high-conductance chemo-optogenetic system based on the vertebrate channel Trpa1b.
Scientific reports7(1)11839(2017 Sep)
Lam PY,Mendu SK,Mills RW,Zheng B,Padilla H,Milan DJ,Desai BN,Peterson RT

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

trpa1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報