FHL2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	FHL2B
Entrez Gene ID	450007
Full Name	four and a half LIM domains 2b
Synonyms	zgc:103466
General protein information	Preferred Names four and a half LIM domains 2b Names four and a half LIM domains 2b
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001006028.2	NP_001006028.2	four and a half LIM domains 2b
XM_017356472.1	XP_017211961.1	four and a half LIM domains 2b isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Lmpt	NP_996110.1
Xenopus tropicalis (tropical clawed frog)	fhl2	NP_001120233.1
Danio rerio (zebrafish)	fhl2a	NP_001003732.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005400	XP_315409.2
Canis lupus familiaris (dog)	FHL2	XP_005626045.1
Rattus norvegicus (Norway rat)	Fhl2	NP_113865.1
Danio rerio (zebrafish)	fhl2b	NP_001006028.2
Caenorhabditis elegans (roundworm)	lim-9	NP_001021410.2
Homo sapiens (human)	FHL2	NP_001034581.1
Pan troglodytes (chimpanzee)	FHL2	XP_530486.3
	FHL2	XP_001109260.1
Bos taurus (cattle)	FHL2	NP_001039511.1
Mus musculus (house mouse)	Fhl2	NP_034342.1
Gallus gallus (chicken)	FHL2	XP_004938568.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Shih YH, Zhang Y, Ding Y, Ross CA, Li H, Olson TM, Xu X
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)

The evolution of cichlid fish egg-spots is linked with a cis-regulatory change.
Santos ME, Braasch I, Boileau N, Meyer BS, Sauteur L, B?hne A, Belting HG, Affolter M, Salzburger W
Nature communications55149(2014 Oct)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

Four-and-a-half LIM domains protein 2 (FHL2) is associated with the development of craniofacial musculature in the teleost fish Sparus aurata.
Rafael MS, Laiz? V, Bensimon-Brito A, Leite RB, Sch?le R, Cancela ML
Cellular and molecular life sciences : CMLS69(3)423-34(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FHL2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FHL2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04177	NM_001006028.2 Latest version!	Danio rerio four and a half LIM domains 2b (fhl2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa04177	XM_017356472.1 Latest version!	Danio rerio four and a half LIM domains 2b (fhl2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FHL2B ( NM_001006028.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001006028.2 Danio rerio four and a half LIM domains 2b (fhl2b), mRNA.

Price & Availability↑

CloneID	ODa04177
Clone ID Related Accession (Same CDS sequence)	NM_001006028.2 , XM_017356472.1
Accession Version	NM_001006028.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Danio rerio(zebrafish)
Product	four and a half LIM domains 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR936426.12. On Jan 9, 2009 this sequence version replaced NM_001006028.1. ##Evidence-Data-START## CDS exon combination :: BC083393.1, DQ118097.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA3505376, SAMEA3505382 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC GCTATGACTG CCACTACTGC AAGGAGTCTC TGTTCGGGAA GAAGTACGTC 
CTGCGGGATG AAAATCCATA CTGCGTGAAG TGCTATGAAA GCCTCTACTC CAATACCTGC 
GAGGAGTGCA AAAAGCCCAT TGGCTGCAAC AGCAGAGATC TGTCTTATAA AGACCGGCAC 
TGGCACGACG ACTGCTTCCA CTGCTTCAAA TGTCACCGTT CTCTTGTGGA CAAGCCATTT 
TCTACCAAGG ACGAGCAGCT ACTGTGTACC GAGTGCTACT CCAATGAGTA TTCCTCTAAA 
TGTTTCGAAT GCAAGAAGAC AATCATGCCA GGCTCCAGGA AGATGGAGCA TAAGGGGAAC 
AGCTGGCATG AGACCTGCTT TACCTGCCAG CGCTGTCAGC AGCCCATTGG AACCAAGAGC 
TTCATTCCTA AGGACAACAG TAACTACTGT GTGCCGTGCT ACGAGAAGCA GTTTGCTCTG 
CAGTGTGTGC AATGCAAGAA GCCAATCACC ACCGGGGGCG TGACCTATCA CGACCAGCCC 
TGGCATAAAG ACTGCTTCCT GTGTACTGGC TGTAAGCAGC AGCTGTCTGG CCAGCGCTTC 
ACCTCTCGTG ATGACTTTCC CTATTGCCTC AACTGCTTCT GCAATCTGTA TGCCAAGAAG 
TGTGCTGCCT GCACCACCCC GATCAGCGGT CTGGGAGGAA GCAAATACAT CTCTTTTGAA 
GAGAGGCAGT GGCACAACGA CTGCTTCAAC TGCAAGAAGT GCTCAGTGTC TCTGGTGGGC 
CGTGGATTCC TGACAGAGAG AGATGATATT CTGTGTCCCG AATGTGGAAA AGACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006028.2
CDS	86..925
Translation	MAERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGKDI

Target ORF information:

RefSeq Version	NM_001006028.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio four and a half LIM domains 2b (fhl2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006028.2

ORF Insert Sequence:

ATGGCGGAGC GCTATGACTG CCACTACTGC AAGGAGTCTC TGTTCGGGAA GAAGTACGTC 
CTGCGGGATG AAAATCCATA CTGCGTGAAG TGCTATGAAA GCCTCTACTC CAATACCTGC 
GAGGAGTGCA AAAAGCCCAT TGGCTGCAAC AGCAGAGATC TGTCTTATAA AGACCGGCAC 
TGGCACGACG ACTGCTTCCA CTGCTTCAAA TGTCACCGTT CTCTTGTGGA CAAGCCATTT 
TCTACCAAGG ACGAGCAGCT ACTGTGTACC GAGTGCTACT CCAATGAGTA TTCCTCTAAA 
TGTTTCGAAT GCAAGAAGAC AATCATGCCA GGCTCCAGGA AGATGGAGCA TAAGGGGAAC 
AGCTGGCATG AGACCTGCTT TACCTGCCAG CGCTGTCAGC AGCCCATTGG AACCAAGAGC 
TTCATTCCTA AGGACAACAG TAACTACTGT GTGCCGTGCT ACGAGAAGCA GTTTGCTCTG 
CAGTGTGTGC AATGCAAGAA GCCAATCACC ACCGGGGGCG TGACCTATCA CGACCAGCCC 
TGGCATAAAG ACTGCTTCCT GTGTACTGGC TGTAAGCAGC AGCTGTCTGG CCAGCGCTTC 
ACCTCTCGTG ATGACTTTCC CTATTGCCTC AACTGCTTCT GCAATCTGTA TGCCAAGAAG 
TGTGCTGCCT GCACCACCCC GATCAGCGGT CTGGGAGGAA GCAAATACAT CTCTTTTGAA 
GAGAGGCAGT GGCACAACGA CTGCTTCAAC TGCAAGAAGT GCTCAGTGTC TCTGGTGGGC 
CGTGGATTCC TGACAGAGAG AGATGATATT CTGTGTCCCG AATGTGGAAA AGACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL2B ( XM_017356472.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211961.1 Danio rerio four and a half LIM domains 2b (fhl2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa04177
Clone ID Related Accession (Same CDS sequence)	NM_001006028.2 , XM_017356472.1
Accession Version	XM_017356472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	four and a half LIM domains 2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGC GCTATGACTG CCACTACTGC AAGGAGTCTC TGTTCGGGAA GAAGTACGTC 
CTGCGGGATG AAAATCCATA CTGCGTGAAG TGCTATGAAA GCCTCTACTC CAATACCTGC 
GAGGAGTGCA AAAAGCCCAT TGGCTGCAAC AGCAGAGATC TGTCTTATAA AGACCGGCAC 
TGGCACGACG ACTGCTTCCA CTGCTTCAAA TGTCACCGTT CTCTTGTGGA CAAGCCATTT 
TCTACCAAGG ACGAGCAGCT ACTGTGTACC GAGTGCTACT CCAATGAGTA TTCCTCTAAA 
TGTTTCGAAT GCAAGAAGAC AATCATGCCA GGCTCCAGGA AGATGGAGCA TAAGGGGAAC 
AGCTGGCATG AGACCTGCTT TACCTGCCAG CGCTGTCAGC AGCCCATTGG AACCAAGAGC 
TTCATTCCTA AGGACAACAG TAACTACTGT GTGCCGTGCT ACGAGAAGCA GTTTGCTCTG 
CAGTGTGTGC AATGCAAGAA GCCAATCACC ACCGGGGGCG TGACCTATCA CGACCAGCCC 
TGGCATAAAG ACTGCTTCCT GTGTACTGGC TGTAAGCAGC AGCTGTCTGG CCAGCGCTTC 
ACCTCTCGTG ATGACTTTCC CTATTGCCTC AACTGCTTCT GCAATCTGTA TGCCAAGAAG 
TGTGCTGCCT GCACCACCCC GATCAGCGGT CTGGGAGGAA GCAAATACAT CTCTTTTGAA 
GAGAGGCAGT GGCACAACGA CTGCTTCAAC TGCAAGAAGT GCTCAGTGTC TCTGGTGGGC 
CGTGGATTCC TGACAGAGAG AGATGATATT CTGTGTCCCG AATGTGGAAA AGACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211961.1
CDS	23..862
Translation	MAERYDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGKDI

Target ORF information:

RefSeq Version	XM_017356472.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio four and a half LIM domains 2b (fhl2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356472.1

ORF Insert Sequence:

ATGGCGGAGC GCTATGACTG CCACTACTGC AAGGAGTCTC TGTTCGGGAA GAAGTACGTC 
CTGCGGGATG AAAATCCATA CTGCGTGAAG TGCTATGAAA GCCTCTACTC CAATACCTGC 
GAGGAGTGCA AAAAGCCCAT TGGCTGCAAC AGCAGAGATC TGTCTTATAA AGACCGGCAC 
TGGCACGACG ACTGCTTCCA CTGCTTCAAA TGTCACCGTT CTCTTGTGGA CAAGCCATTT 
TCTACCAAGG ACGAGCAGCT ACTGTGTACC GAGTGCTACT CCAATGAGTA TTCCTCTAAA 
TGTTTCGAAT GCAAGAAGAC AATCATGCCA GGCTCCAGGA AGATGGAGCA TAAGGGGAAC 
AGCTGGCATG AGACCTGCTT TACCTGCCAG CGCTGTCAGC AGCCCATTGG AACCAAGAGC 
TTCATTCCTA AGGACAACAG TAACTACTGT GTGCCGTGCT ACGAGAAGCA GTTTGCTCTG 
CAGTGTGTGC AATGCAAGAA GCCAATCACC ACCGGGGGCG TGACCTATCA CGACCAGCCC 
TGGCATAAAG ACTGCTTCCT GTGTACTGGC TGTAAGCAGC AGCTGTCTGG CCAGCGCTTC 
ACCTCTCGTG ATGACTTTCC CTATTGCCTC AACTGCTTCT GCAATCTGTA TGCCAAGAAG 
TGTGCTGCCT GCACCACCCC GATCAGCGGT CTGGGAGGAA GCAAATACAT CTCTTTTGAA 
GAGAGGCAGT GGCACAACGA CTGCTTCAAC TGCAAGAAGT GCTCAGTGTC TCTGGTGGGC 
CGTGGATTCC TGACAGAGAG AGATGATATT CTGTGTCCCG AATGTGGAAA AGACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)
Shih YH,Zhang Y,Ding Y,Ross CA,Li H,Olson TM,Xu X

The evolution of cichlid fish egg-spots is linked with a cis-regulatory change.
Nature communications55149(2014 Oct)
Santos ME,Braasch I,Boileau N,Meyer BS,Sauteur L,B?hne A,Belting HG,Affolter M,Salzburger W

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

Four-and-a-half LIM domains protein 2 (FHL2) is associated with the development of craniofacial musculature in the teleost fish Sparus aurata.
Cellular and molecular life sciences : CMLS69(3)423-34(2012 Feb)
Rafael MS,Laiz? V,Bensimon-Brito A,Leite RB,Sch?le R,Cancela ML

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FHL2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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