RIC8A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	RIC8A
Entrez Gene ID	449546
Full Name	RIC8 guanine nucleotide exchange factor A
Synonyms	zgc:92294
General protein information	Preferred Names RIC8 guanine nucleotide exchange factor A Names synembryn-A resistance to inhibitors of cholinesterase 8 homolog A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001005588.1	NP_001005588.1	synembryn-A
XM_005162988.4	XP_005163045.1	synembryn-A isoform X1
XM_005162988.3	XP_005163045.1	synembryn-A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	RIC8A	NP_001273063.1
Rattus norvegicus (Norway rat)	Ric8a	NP_001093990.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010793	XP_318515.3
Canis lupus familiaris (dog)	RIC8A	XP_005631129.1
Mus musculus (house mouse)	Ric8	NP_444424.1
	RIC8A	XP_001083119.2
Bos taurus (cattle)	RIC8A	NP_001015627.1
Danio rerio (zebrafish)	ric8a	NP_001005588.1
Pan troglodytes (chimpanzee)	RIC8A	XP_521700.2
Drosophila melanogaster (fruit fly)	ric8a	NP_572550.2
Xenopus tropicalis (tropical clawed frog)	ric8a	NP_989159.1
Gallus gallus (chicken)	RIC8A	NP_001026345.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish.
Seiler C, Gebhart N, Zhang Y, Shinton SA, Li YS, Ross NL, Liu X, Li Q, Bilbee AN, Varshney GK, LaFave MC, Burgess SM, Balciuniene J, Balciunas D, Hardy RR, Kappes DJ, Wiest DL, Rhodes J
PloS one10(7)e0131908(2015)

Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like.
Nagayoshi S, Hayashi E, Abe G, Osato N, Asakawa K, Urasaki A, Horikawa K, Ikeo K, Takeda H, Kawakami K
Development (Cambridge, England)135(1)159-69(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RIC8A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RIC8A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02692	NM_001005588.1 Latest version!	Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa29177	XM_005162988.4 Latest version!	Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa29177	XM_005162988.3	Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RIC8A ( NM_001005588.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001005588.1 Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), mRNA.

Price & Availability↑

CloneID	ODa02692
Clone ID Related Accession (Same CDS sequence)	NM_001005588.1
Accession Version	NM_001005588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Danio rerio(zebrafish)
Product	synembryn-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081640.1. ##Evidence-Data-START## Transcript exon combination :: BC081640.1, AB354735.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGACTTAA ATGCCATCAT AGAGAAAATG GAAACGGGCG ACCAGGACGC CGCACTGACA 
GCTTTACAGA CATATAATAA AGAGAAATCA CAGTGCTTTT CATTCACCTC TGGAGAAGAG 
GAGGACAGAG AGCGTCTGGG TGAGTTAGTG TTAAGCTTTT TGGAGAGAGA CCTCCAGCCA 
TCCTGCCAGC TGGCCTGCCT AGAGACCATC CGCATCCTGT CCCGGGACAA GAAGAGCCTG 
TCTCCGTTCG CCACCCGCCA TGCTATGCAG ATCCTGATTC GACATGCAGG GCTTGGGCAG 
GGAGAGGGCG TCACCCCAGA GATCCCAGAT TTAGAGGTGA TCGTGGAGGC TCTGAAGTGC 
TTATGCAACA TCGTGTTTAA CAGCGAAGCA GCGCAGGAGG CCGCAGCTGA CCTGCAGTTG 
ATGGTGGGTT TAGCAGAGCG GCTGAAACAG TGCCGGGAAC CACAGTGGAA CCATGACGTG 
CGTTTCTTCG ATCTGCGCCT TACGTTCCTG ATCACCGCCC TGCGTGTGGA CGTGCGGGCT 
CAGCTGGCAC ACGAGCTGCG CGGGGTCAGT CTGCTGTCCG AGGCTTTGGA CGCCACCTTT 
GGACTCTGCT GGCCGGACAT GTATGAGGTG GCGCGGGCTG GGTTCGACGG CTGCTCAGAG 
CTGCCCCCGC TGGGCAGACA AGAGACCGAG AGAGTCATGG AGATCCTCAA GATCCTCTTC 
AATGTTACGT TTGATTCCAA CCGACGCCAT GTGGACGAGG AGGAAGCAGC CACATACAGA 
CACCTGGGAG CCATACTGAG ACACTGCATC ATGAGCAGCG CAGAGGGAGA GGAGCGAACA 
GAGGAGATGC ACAGCCACAC AGTGAACCTT CTGGGAAACT TGCCGCTTCC GTGTCTGGAC 
GTCCTGCTGA TGCCTAAAGT CCAGCAGGGC TCCATCGAGT ACATGGGAGT CAACATGGAT 
GCTGTCAAAG TGCTGGTGGA GTTCATGGAG AAAAGACTCG ACCGGGGAAA CAAGCTAAAG 
GAGACGCTTC TGCCCTCGCT GAATCTTCTA ACCGAGAGCG CTCGCATCCA CAGAGAGACC 
AGGAAGTTTT TACGGAATAA AGTGCTTCCC CCATTACGAG ATGTCAAAAA CAGACCTGAG 
GTCGGTAATG CTTTACGAAA TAAGCTGGTT CGTCTGATGA CCCACATCGA CACAGATGTA 
AAGCACTGCG CGGCTGAGTT CCTGTTTGTG CTCTGCAAGG AAAGCGTGTC TAGGTTTATC 
AAGTACACGG GTTATGGTAA TGCAGCAGGA CTGCTGGCCG CCCGGGGACT GATGAGAGGC 
GGTCGAGACC CAGGACATTA TTCAGAAGAT GAGGATAGTG ATACAGAAGA GTACAGAGAG 
GCCAAACCAC ACATCAATCC AGTGACTGGT CGCGTGGAGG AAGAACAGCC AAACCCTATG 
GAGGGAATGA CTGATGAACA GAAGGAATAT GAAGCCATGA AACTGGTCAA CATGTTCGAC 
AAACTCTCCA GGGAGCAGGT GATCCAACCA ATGAAGATCG GAGCTGATGG TAAAATGACT 
TCAATGGAAC CACACGAACT CCATCATTTA GCCAGCCAAC AGTTCGGAGA GTCCAATAAT 
TCTGACAGCG ACAGCGATAC AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005588.1
CDS	32..1678
Translation	MDLNAIIEKMETGDQDAALTALQTYNKEKSQCFSFTSGEEEDRERLGELVLSFLERDLQPSCQLACLETIRILSRDKKSLSPFATRHAMQILIRHAGLGQGEGVTPEIPDLEVIVEALKCLCNIVFNSEAAQEAAADLQLMVGLAERLKQCREPQWNHDVRFFDLRLTFLITALRVDVRAQLAHELRGVSLLSEALDATFGLCWPDMYEVARAGFDGCSELPPLGRQETERVMEILKILFNVTFDSNRRHVDEEEAATYRHLGAILRHCIMSSAEGEERTEEMHSHTVNLLGNLPLPCLDVLLMPKVQQGSIEYMGVNMDAVKVLVEFMEKRLDRGNKLKETLLPSLNLLTESARIHRETRKFLRNKVLPPLRDVKNRPEVGNALRNKLVRLMTHIDTDVKHCAAEFLFVLCKESVSRFIKYTGYGNAAGLLAARGLMRGGRDPGHYSEDEDSDTEEYREAKPHINPVTGRVEEEQPNPMEGMTDEQKEYEAMKLVNMFDKLSREQVIQPMKIGADGKMTSMEPHELHHLASQQFGESNNSDSDSDTN

Target ORF information:

RefSeq Version	NM_001005588.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005588.1

ORF Insert Sequence:

ATGGACTTAA ATGCCATCAT AGAGAAAATG GAAACGGGCG ACCAGGACGC CGCACTGACA 
GCTTTACAGA CATATAATAA AGAGAAATCA CAGTGCTTTT CATTCACCTC TGGAGAAGAG 
GAGGACAGAG AGCGTCTGGG TGAGTTAGTG TTAAGCTTTT TGGAGAGAGA CCTCCAGCCA 
TCCTGCCAGC TGGCCTGCCT AGAGACCATC CGCATCCTGT CCCGGGACAA GAAGAGCCTG 
TCTCCGTTCG CCACCCGCCA TGCTATGCAG ATCCTGATTC GACATGCAGG GCTTGGGCAG 
GGAGAGGGCG TCACCCCAGA GATCCCAGAT TTAGAGGTGA TCGTGGAGGC TCTGAAGTGC 
TTATGCAACA TCGTGTTTAA CAGCGAAGCA GCGCAGGAGG CCGCAGCTGA CCTGCAGTTG 
ATGGTGGGTT TAGCAGAGCG GCTGAAACAG TGCCGGGAAC CACAGTGGAA CCATGACGTG 
CGTTTCTTCG ATCTGCGCCT TACGTTCCTG ATCACCGCCC TGCGTGTGGA CGTGCGGGCT 
CAGCTGGCAC ACGAGCTGCG CGGGGTCAGT CTGCTGTCCG AGGCTTTGGA CGCCACCTTT 
GGACTCTGCT GGCCGGACAT GTATGAGGTG GCGCGGGCTG GGTTCGACGG CTGCTCAGAG 
CTGCCCCCGC TGGGCAGACA AGAGACCGAG AGAGTCATGG AGATCCTCAA GATCCTCTTC 
AATGTTACGT TTGATTCCAA CCGACGCCAT GTGGACGAGG AGGAAGCAGC CACATACAGA 
CACCTGGGAG CCATACTGAG ACACTGCATC ATGAGCAGCG CAGAGGGAGA GGAGCGAACA 
GAGGAGATGC ACAGCCACAC AGTGAACCTT CTGGGAAACT TGCCGCTTCC GTGTCTGGAC 
GTCCTGCTGA TGCCTAAAGT CCAGCAGGGC TCCATCGAGT ACATGGGAGT CAACATGGAT 
GCTGTCAAAG TGCTGGTGGA GTTCATGGAG AAAAGACTCG ACCGGGGAAA CAAGCTAAAG 
GAGACGCTTC TGCCCTCGCT GAATCTTCTA ACCGAGAGCG CTCGCATCCA CAGAGAGACC 
AGGAAGTTTT TACGGAATAA AGTGCTTCCC CCATTACGAG ATGTCAAAAA CAGACCTGAG 
GTCGGTAATG CTTTACGAAA TAAGCTGGTT CGTCTGATGA CCCACATCGA CACAGATGTA 
AAGCACTGCG CGGCTGAGTT CCTGTTTGTG CTCTGCAAGG AAAGCGTGTC TAGGTTTATC 
AAGTACACGG GTTATGGTAA TGCAGCAGGA CTGCTGGCCG CCCGGGGACT GATGAGAGGC 
GGTCGAGACC CAGGACATTA TTCAGAAGAT GAGGATAGTG ATACAGAAGA GTACAGAGAG 
GCCAAACCAC ACATCAATCC AGTGACTGGT CGCGTGGAGG AAGAACAGCC AAACCCTATG 
GAGGGAATGA CTGATGAACA GAAGGAATAT GAAGCCATGA AACTGGTCAA CATGTTCGAC 
AAACTCTCCA GGGAGCAGGT GATCCAACCA ATGAAGATCG GAGCTGATGG TAAAATGACT 
TCAATGGAAC CACACGAACT CCATCATTTA GCCAGCCAAC AGTTCGGAGA GTCCAATAAT 
TCTGACAGCG ACAGCGATAC AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIC8A ( XM_005162988.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163045.1 Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29177
Clone ID Related Accession (Same CDS sequence)	XM_005162988.4 , XM_005162988.3
Accession Version	XM_005162988.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	synembryn-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162988.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG ACTTAAATGC CATCATAGAG AAAATGGAAA CGGGCGACCA GGACGCCGCA 
CTGACAGCTT TACAGACATA TAATAAAGAG AAATCACAGT GCTTTTCATT CACCTCTGGA 
GAAGAGGAGG ACAGAGAGGA TGGAGATATG ATCGAGCGTC TGGGTGAGTT AGTGTTAAGC 
TTTTTGGAGA GAGACCTCCA GCCATCCTGC CAGCTGGCCT GCCTAGAGAC CATCCGCATC 
CTGTCCCGGG ACAAGAAGAG CCTGTCTCCG TTCGCCACCC GCCATGCTAT GCAGATCCTG 
ATTCGACATG CAGGGCTTGG GCAGGGAGAG GGCGTCACCC CAGAGATCCC AGATTTAGAG 
GTGATCGTGG AGGCTCTGAA GTGCTTATGC AACATCGTGT TTAACAGCGA AGCAGCGCAG 
GAGGCCGCAG CTGACCTGCA GTTGATGGTG GGTTTAGCAG AGCGGCTGAA ACAGTGCCGG 
GAACCACAGT GGAACCATGA CGTGCGTTTC TTCGATCTGC GCCTTACGTT CCTGATCACC 
GCCCTGCGTG TGGACGTGCG GGCTCAGCTG GCACACGAGC TGCGCGGGGT CAGTCTGCTG 
TCCGAGGCTT TGGACGCCAC CTTTGGACTC TGCTGGCCGG ACATGTATGA GGTGGCGCGG 
GCTGGGTTCG ACGGCTGCTC AGAGCTGCCC CCGCTGGGCA GACAAGAGAC CGAGAGAGTC 
ATGGAGATCC TCAAGATCCT CTTCAATGTT ACGTTTGATT CCAACCGACG CCATGTGGAC 
GAGGAGGAAG CAGCCACATA CAGACACCTG GGAGCCATAC TGAGACACTG CATCATGAGC 
AGCGCAGAGG GAGAGGAGCG AACAGAGGAG ATGCACAGCC ACACAGTGAA CCTTCTGGGA 
AACTTGCCGC TTCCGTGTCT GGACGTCCTG CTGATGCCTA AAGTCCAGCA GGGCTCCATC 
GAGTACATGG GAGTCAACAT GGATGCTGTC AAAGTGCTGG TGGAGTTCAT GGAGAAAAGA 
CTCGACCGGG GAAACAAGCT AAAGGAGACG CTTCTGCCCT CGCTGAATCT TCTAACCGAG 
AGCGCTCGCA TCCACAGAGA GACCAGGAAG TTTTTACGGA ATAAAGTGCT TCCCCCATTA 
CGAGATGTCA AAAACAGACC TGAGGTCGGT AATGCTTTAC GAAATAAGCT GGTTCGTCTG 
ATGACCCACA TCGACACAGA TGTAAAGCAC TGCGCGGCTG AGTTCCTGTT TGTGCTCTGC 
AAGGAAAGCG TGTCTAGGTT TATCAAGTAC ACGGGTTATG GTAATGCAGC AGGACTGCTG 
GCCGCCCGGG GACTGATGAG AGGCGGTCGA GACCCAGGAC ATTATTCAGA AGATGAGGAT 
AGTGATACAG AAGAGTACAG AGAGGCCAAA CCACACATCA ATCCAGTGAC TGGTCGCGTG 
GAGGAAGAAC AGCCAAACCC TATGGAGGGA ATGACTGATG AACAGAAGGA ATATGAAGCC 
ATGAAACTGG TCAACATGTT CGACAAACTC TCCAGGGAGC AGGTGATCCA ACCAATGAAG 
ATCGGAGCTG ATGGTAAAAT GACTTCAATG GAACCACACG AACTCCATCA TTTAGCCAGC 
CAACAGTTCG GAGAGTCCAA TAATTCTGAC AGCGACAGCG ATACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163045.1
CDS	118..1788
Translation	MKMDLNAIIEKMETGDQDAALTALQTYNKEKSQCFSFTSGEEEDREDGDMIERLGELVLSFLERDLQPSCQLACLETIRILSRDKKSLSPFATRHAMQILIRHAGLGQGEGVTPEIPDLEVIVEALKCLCNIVFNSEAAQEAAADLQLMVGLAERLKQCREPQWNHDVRFFDLRLTFLITALRVDVRAQLAHELRGVSLLSEALDATFGLCWPDMYEVARAGFDGCSELPPLGRQETERVMEILKILFNVTFDSNRRHVDEEEAATYRHLGAILRHCIMSSAEGEERTEEMHSHTVNLLGNLPLPCLDVLLMPKVQQGSIEYMGVNMDAVKVLVEFMEKRLDRGNKLKETLLPSLNLLTESARIHRETRKFLRNKVLPPLRDVKNRPEVGNALRNKLVRLMTHIDTDVKHCAAEFLFVLCKESVSRFIKYTGYGNAAGLLAARGLMRGGRDPGHYSEDEDSDTEEYREAKPHINPVTGRVEEEQPNPMEGMTDEQKEYEAMKLVNMFDKLSREQVIQPMKIGADGKMTSMEPHELHHLASQQFGESNNSDSDSDTN

Target ORF information:

RefSeq Version	XM_005162988.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162988.4

ORF Insert Sequence:

ATGAAAATGG ACTTAAATGC CATCATAGAG AAAATGGAAA CGGGCGACCA GGACGCCGCA 
CTGACAGCTT TACAGACATA TAATAAAGAG AAATCACAGT GCTTTTCATT CACCTCTGGA 
GAAGAGGAGG ACAGAGAGGA TGGAGATATG ATCGAGCGTC TGGGTGAGTT AGTGTTAAGC 
TTTTTGGAGA GAGACCTCCA GCCATCCTGC CAGCTGGCCT GCCTAGAGAC CATCCGCATC 
CTGTCCCGGG ACAAGAAGAG CCTGTCTCCG TTCGCCACCC GCCATGCTAT GCAGATCCTG 
ATTCGACATG CAGGGCTTGG GCAGGGAGAG GGCGTCACCC CAGAGATCCC AGATTTAGAG 
GTGATCGTGG AGGCTCTGAA GTGCTTATGC AACATCGTGT TTAACAGCGA AGCAGCGCAG 
GAGGCCGCAG CTGACCTGCA GTTGATGGTG GGTTTAGCAG AGCGGCTGAA ACAGTGCCGG 
GAACCACAGT GGAACCATGA CGTGCGTTTC TTCGATCTGC GCCTTACGTT CCTGATCACC 
GCCCTGCGTG TGGACGTGCG GGCTCAGCTG GCACACGAGC TGCGCGGGGT CAGTCTGCTG 
TCCGAGGCTT TGGACGCCAC CTTTGGACTC TGCTGGCCGG ACATGTATGA GGTGGCGCGG 
GCTGGGTTCG ACGGCTGCTC AGAGCTGCCC CCGCTGGGCA GACAAGAGAC CGAGAGAGTC 
ATGGAGATCC TCAAGATCCT CTTCAATGTT ACGTTTGATT CCAACCGACG CCATGTGGAC 
GAGGAGGAAG CAGCCACATA CAGACACCTG GGAGCCATAC TGAGACACTG CATCATGAGC 
AGCGCAGAGG GAGAGGAGCG AACAGAGGAG ATGCACAGCC ACACAGTGAA CCTTCTGGGA 
AACTTGCCGC TTCCGTGTCT GGACGTCCTG CTGATGCCTA AAGTCCAGCA GGGCTCCATC 
GAGTACATGG GAGTCAACAT GGATGCTGTC AAAGTGCTGG TGGAGTTCAT GGAGAAAAGA 
CTCGACCGGG GAAACAAGCT AAAGGAGACG CTTCTGCCCT CGCTGAATCT TCTAACCGAG 
AGCGCTCGCA TCCACAGAGA GACCAGGAAG TTTTTACGGA ATAAAGTGCT TCCCCCATTA 
CGAGATGTCA AAAACAGACC TGAGGTCGGT AATGCTTTAC GAAATAAGCT GGTTCGTCTG 
ATGACCCACA TCGACACAGA TGTAAAGCAC TGCGCGGCTG AGTTCCTGTT TGTGCTCTGC 
AAGGAAAGCG TGTCTAGGTT TATCAAGTAC ACGGGTTATG GTAATGCAGC AGGACTGCTG 
GCCGCCCGGG GACTGATGAG AGGCGGTCGA GACCCAGGAC ATTATTCAGA AGATGAGGAT 
AGTGATACAG AAGAGTACAG AGAGGCCAAA CCACACATCA ATCCAGTGAC TGGTCGCGTG 
GAGGAAGAAC AGCCAAACCC TATGGAGGGA ATGACTGATG AACAGAAGGA ATATGAAGCC 
ATGAAACTGG TCAACATGTT CGACAAACTC TCCAGGGAGC AGGTGATCCA ACCAATGAAG 
ATCGGAGCTG ATGGTAAAAT GACTTCAATG GAACCACACG AACTCCATCA TTTAGCCAGC 
CAACAGTTCG GAGAGTCCAA TAATTCTGAC AGCGACAGCG ATACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RIC8A ( XM_005162988.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005163045.1 Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29177
Clone ID Related Accession (Same CDS sequence)	XM_005162988.4 , XM_005162988.3
Accession Version	XM_005162988.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	synembryn-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATGG ACTTAAATGC CATCATAGAG AAAATGGAAA CGGGCGACCA GGACGCCGCA 
CTGACAGCTT TACAGACATA TAATAAAGAG AAATCACAGT GCTTTTCATT CACCTCTGGA 
GAAGAGGAGG ACAGAGAGGA TGGAGATATG ATCGAGCGTC TGGGTGAGTT AGTGTTAAGC 
TTTTTGGAGA GAGACCTCCA GCCATCCTGC CAGCTGGCCT GCCTAGAGAC CATCCGCATC 
CTGTCCCGGG ACAAGAAGAG CCTGTCTCCG TTCGCCACCC GCCATGCTAT GCAGATCCTG 
ATTCGACATG CAGGGCTTGG GCAGGGAGAG GGCGTCACCC CAGAGATCCC AGATTTAGAG 
GTGATCGTGG AGGCTCTGAA GTGCTTATGC AACATCGTGT TTAACAGCGA AGCAGCGCAG 
GAGGCCGCAG CTGACCTGCA GTTGATGGTG GGTTTAGCAG AGCGGCTGAA ACAGTGCCGG 
GAACCACAGT GGAACCATGA CGTGCGTTTC TTCGATCTGC GCCTTACGTT CCTGATCACC 
GCCCTGCGTG TGGACGTGCG GGCTCAGCTG GCACACGAGC TGCGCGGGGT CAGTCTGCTG 
TCCGAGGCTT TGGACGCCAC CTTTGGACTC TGCTGGCCGG ACATGTATGA GGTGGCGCGG 
GCTGGGTTCG ACGGCTGCTC AGAGCTGCCC CCGCTGGGCA GACAAGAGAC CGAGAGAGTC 
ATGGAGATCC TCAAGATCCT CTTCAATGTT ACGTTTGATT CCAACCGACG CCATGTGGAC 
GAGGAGGAAG CAGCCACATA CAGACACCTG GGAGCCATAC TGAGACACTG CATCATGAGC 
AGCGCAGAGG GAGAGGAGCG AACAGAGGAG ATGCACAGCC ACACAGTGAA CCTTCTGGGA 
AACTTGCCGC TTCCGTGTCT GGACGTCCTG CTGATGCCTA AAGTCCAGCA GGGCTCCATC 
GAGTACATGG GAGTCAACAT GGATGCTGTC AAAGTGCTGG TGGAGTTCAT GGAGAAAAGA 
CTCGACCGGG GAAACAAGCT AAAGGAGACG CTTCTGCCCT CGCTGAATCT TCTAACCGAG 
AGCGCTCGCA TCCACAGAGA GACCAGGAAG TTTTTACGGA ATAAAGTGCT TCCCCCATTA 
CGAGATGTCA AAAACAGACC TGAGGTCGGT AATGCTTTAC GAAATAAGCT GGTTCGTCTG 
ATGACCCACA TCGACACAGA TGTAAAGCAC TGCGCGGCTG AGTTCCTGTT TGTGCTCTGC 
AAGGAAAGCG TGTCTAGGTT TATCAAGTAC ACGGGTTATG GTAATGCAGC AGGACTGCTG 
GCCGCCCGGG GACTGATGAG AGGCGGTCGA GACCCAGGAC ATTATTCAGA AGATGAGGAT 
AGTGATACAG AAGAGTACAG AGAGGCCAAA CCACACATCA ATCCAGTGAC TGGTCGCGTG 
GAGGAAGAAC AGCCAAACCC TATGGAGGGA ATGACTGATG AACAGAAGGA ATATGAAGCC 
ATGAAACTGG TCAACATGTT CGACAAACTC TCCAGGGAGC AGGTGATCCA ACCAATGAAG 
ATCGGAGCTG ATGGTAAAAT GACTTCAATG GAACCACACG AACTCCATCA TTTAGCCAGC 
CAACAGTTCG GAGAGTCCAA TAATTCTGAC AGCGACAGCG ATACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005163045.1
CDS	106..1776
Translation	MKMDLNAIIEKMETGDQDAALTALQTYNKEKSQCFSFTSGEEEDREDGDMIERLGELVLSFLERDLQPSCQLACLETIRILSRDKKSLSPFATRHAMQILIRHAGLGQGEGVTPEIPDLEVIVEALKCLCNIVFNSEAAQEAAADLQLMVGLAERLKQCREPQWNHDVRFFDLRLTFLITALRVDVRAQLAHELRGVSLLSEALDATFGLCWPDMYEVARAGFDGCSELPPLGRQETERVMEILKILFNVTFDSNRRHVDEEEAATYRHLGAILRHCIMSSAEGEERTEEMHSHTVNLLGNLPLPCLDVLLMPKVQQGSIEYMGVNMDAVKVLVEFMEKRLDRGNKLKETLLPSLNLLTESARIHRETRKFLRNKVLPPLRDVKNRPEVGNALRNKLVRLMTHIDTDVKHCAAEFLFVLCKESVSRFIKYTGYGNAAGLLAARGLMRGGRDPGHYSEDEDSDTEEYREAKPHINPVTGRVEEEQPNPMEGMTDEQKEYEAMKLVNMFDKLSREQVIQPMKIGADGKMTSMEPHELHHLASQQFGESNNSDSDSDTN

Target ORF information:

RefSeq Version	XM_005162988.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RIC8 guanine nucleotide exchange factor A (ric8a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162988.3

ORF Insert Sequence:

ATGAAAATGG ACTTAAATGC CATCATAGAG AAAATGGAAA CGGGCGACCA GGACGCCGCA 
CTGACAGCTT TACAGACATA TAATAAAGAG AAATCACAGT GCTTTTCATT CACCTCTGGA 
GAAGAGGAGG ACAGAGAGGA TGGAGATATG ATCGAGCGTC TGGGTGAGTT AGTGTTAAGC 
TTTTTGGAGA GAGACCTCCA GCCATCCTGC CAGCTGGCCT GCCTAGAGAC CATCCGCATC 
CTGTCCCGGG ACAAGAAGAG CCTGTCTCCG TTCGCCACCC GCCATGCTAT GCAGATCCTG 
ATTCGACATG CAGGGCTTGG GCAGGGAGAG GGCGTCACCC CAGAGATCCC AGATTTAGAG 
GTGATCGTGG AGGCTCTGAA GTGCTTATGC AACATCGTGT TTAACAGCGA AGCAGCGCAG 
GAGGCCGCAG CTGACCTGCA GTTGATGGTG GGTTTAGCAG AGCGGCTGAA ACAGTGCCGG 
GAACCACAGT GGAACCATGA CGTGCGTTTC TTCGATCTGC GCCTTACGTT CCTGATCACC 
GCCCTGCGTG TGGACGTGCG GGCTCAGCTG GCACACGAGC TGCGCGGGGT CAGTCTGCTG 
TCCGAGGCTT TGGACGCCAC CTTTGGACTC TGCTGGCCGG ACATGTATGA GGTGGCGCGG 
GCTGGGTTCG ACGGCTGCTC AGAGCTGCCC CCGCTGGGCA GACAAGAGAC CGAGAGAGTC 
ATGGAGATCC TCAAGATCCT CTTCAATGTT ACGTTTGATT CCAACCGACG CCATGTGGAC 
GAGGAGGAAG CAGCCACATA CAGACACCTG GGAGCCATAC TGAGACACTG CATCATGAGC 
AGCGCAGAGG GAGAGGAGCG AACAGAGGAG ATGCACAGCC ACACAGTGAA CCTTCTGGGA 
AACTTGCCGC TTCCGTGTCT GGACGTCCTG CTGATGCCTA AAGTCCAGCA GGGCTCCATC 
GAGTACATGG GAGTCAACAT GGATGCTGTC AAAGTGCTGG TGGAGTTCAT GGAGAAAAGA 
CTCGACCGGG GAAACAAGCT AAAGGAGACG CTTCTGCCCT CGCTGAATCT TCTAACCGAG 
AGCGCTCGCA TCCACAGAGA GACCAGGAAG TTTTTACGGA ATAAAGTGCT TCCCCCATTA 
CGAGATGTCA AAAACAGACC TGAGGTCGGT AATGCTTTAC GAAATAAGCT GGTTCGTCTG 
ATGACCCACA TCGACACAGA TGTAAAGCAC TGCGCGGCTG AGTTCCTGTT TGTGCTCTGC 
AAGGAAAGCG TGTCTAGGTT TATCAAGTAC ACGGGTTATG GTAATGCAGC AGGACTGCTG 
GCCGCCCGGG GACTGATGAG AGGCGGTCGA GACCCAGGAC ATTATTCAGA AGATGAGGAT 
AGTGATACAG AAGAGTACAG AGAGGCCAAA CCACACATCA ATCCAGTGAC TGGTCGCGTG 
GAGGAAGAAC AGCCAAACCC TATGGAGGGA ATGACTGATG AACAGAAGGA ATATGAAGCC 
ATGAAACTGG TCAACATGTT CGACAAACTC TCCAGGGAGC AGGTGATCCA ACCAATGAAG 
ATCGGAGCTG ATGGTAAAAT GACTTCAATG GAACCACACG AACTCCATCA TTTAGCCAGC 
CAACAGTTCG GAGAGTCCAA TAATTCTGAC AGCGACAGCG ATACAAACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutagenesis Screen Identifies agtpbp1 and eps15L1 as Essential for T lymphocyte Development in Zebrafish.
PloS one10(7)e0131908(2015)
Seiler C,Gebhart N,Zhang Y,Shinton SA,Li YS,Ross NL,Liu X,Li Q,Bilbee AN,Varshney GK,LaFave MC,Burgess SM,Balciuniene J,Balciunas D,Hardy RR,Kappes DJ,Wiest DL,Rhodes J

Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like.
Development (Cambridge, England)135(1)159-69(2008 Jan)
Nagayoshi S,Hayashi E,Abe G,Osato N,Asakawa K,Urasaki A,Horikawa K,Ikeo K,Takeda H,Kawakami K

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RIC8A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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