nhsl1b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009294777.3	XP_009293052.1	NHS-like protein 1 isoform X6
XM_003200723.4	XP_003200771.1	NHS-like protein 1 isoform X7
XM_009294777.2	XP_009293052.1	NHS-like protein 1 isoform X6
XM_005160764.3	XP_005160821.1	NHS-like protein 1 isoform X1
XM_005160765.3	XP_005160822.1	NHS-like protein 1 isoform X2
XM_005160764.4	XP_005160821.1	NHS-like protein 1 isoform X1
XM_005160765.4	XP_005160822.1	NHS-like protein 1 isoform X2
XM_005160766.4	XP_005160823.1	NHS-like protein 1 isoform X3
XM_005160767.4	XP_005160824.1	NHS-like protein 1 isoform X4
XM_005160766.3	XP_005160823.1	NHS-like protein 1 isoform X3
XM_005160767.3	XP_005160824.1	NHS-like protein 1 isoform X4
XM_005160768.3	XP_005160825.3	NHS-like protein 1 isoform X5
XM_005160768.4	XP_005160825.3	NHS-like protein 1 isoform X5
XM_003200723.5	XP_003200771.1	NHS-like protein 1 isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa41206	XM_009294777.3 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
ODa56658	XM_005160768.4 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa25046	XM_005160764.4 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25047	XM_005160765.4 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25048	XM_005160766.4 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25049	XM_005160767.4 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25051	XM_003200723.5 Latest version!	Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa41206	XM_009294777.2	Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$881.30 $1259.00
ODa25046	XM_005160764.3	Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25047	XM_005160765.3	Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25048	XM_005160766.3	Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25049	XM_005160767.3	Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa56658	XM_005160768.3	Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa25051	XM_003200723.4	Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

nhsl1b ( XM_009294777.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293052.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa41206
Clone ID Related Accession (Same CDS sequence)	XM_009294777.3 , XM_009294777.2
Accession Version	XM_009294777.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG 
CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG 
GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC 
CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT 
GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG 
GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG 
GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG 
AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG 
TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA 
CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA 
CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG 
TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA 
GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG 
GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC 
ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG 
TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT 
GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT 
AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG 
GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT 
TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA 
CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT 
GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA 
GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC 
ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC 
ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC 
TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC 
CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC 
TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT 
GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA 
GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG 
CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA 
AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT 
GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC 
TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA 
AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT 
GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT 
TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG 
TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC 
CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT 
AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT 
GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA 
TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT 
TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT 
AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT 
AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG 
AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC 
CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC 
GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA 
CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA 
CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA 
AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA 
TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC 
AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT 
ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG 
GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC 
TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG 
ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA 
ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT 
TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT 
GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA 
AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG 
AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA 
GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC 
GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG 
CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG 
CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA 
GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC 
CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT 
CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC 
TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC 
CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA 
ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA 
TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC 
ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA 
CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293052.1
CDS	32..4588
Translation	MPVKGISFWGHRKRKSPYSAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_009294777.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294777.3

ORF Insert Sequence:

ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG 
CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG 
GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC 
CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT 
GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG 
GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG 
GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG 
AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG 
TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA 
CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA 
CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG 
TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA 
GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG 
GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC 
ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG 
TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT 
GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT 
AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG 
GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT 
TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA 
CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT 
GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA 
GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC 
ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC 
ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC 
TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC 
CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC 
TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT 
GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA 
GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG 
CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA 
AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT 
GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC 
TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA 
AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT 
GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT 
TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG 
TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC 
CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT 
AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT 
GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA 
TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT 
TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT 
AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT 
AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG 
AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC 
CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC 
GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA 
CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA 
CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA 
AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA 
TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC 
AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT 
ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG 
GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC 
TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG 
ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA 
ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT 
TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT 
GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA 
AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG 
AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA 
GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC 
GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG 
CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG 
CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA 
GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC 
CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT 
CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC 
TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC 
CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA 
ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA 
TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC 
ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA 
CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_009294777.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293052.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa41206
Clone ID Related Accession (Same CDS sequence)	XM_009294777.3 , XM_009294777.2
Accession Version	XM_009294777.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4557bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG 
CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG 
GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC 
CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT 
GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG 
GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG 
GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG 
AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG 
TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA 
CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA 
CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG 
TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA 
GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG 
GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC 
ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG 
TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT 
GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT 
AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG 
GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT 
TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA 
CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT 
GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA 
GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC 
ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC 
ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC 
TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC 
CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC 
TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT 
GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA 
GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG 
CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA 
AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT 
GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC 
TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA 
AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT 
GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT 
TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG 
TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC 
CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT 
AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT 
GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA 
TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT 
TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT 
AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT 
AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG 
AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC 
CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC 
GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA 
CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA 
CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA 
AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA 
TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC 
AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT 
ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG 
GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC 
TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG 
ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA 
ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT 
TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT 
GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA 
AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG 
AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA 
GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC 
GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG 
CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG 
CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA 
GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC 
CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT 
CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC 
TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC 
CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA 
ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA 
TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC 
ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA 
CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293052.1
CDS	16..4572
Translation	MPVKGISFWGHRKRKSPYSAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_009294777.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294777.2

ORF Insert Sequence:

ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG 
CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG 
GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC 
CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT 
GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG 
GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG 
GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG 
AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG 
TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA 
CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA 
CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG 
TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA 
GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG 
GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC 
ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG 
TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT 
GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT 
AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG 
GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT 
TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA 
CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT 
GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA 
GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC 
ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC 
ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC 
TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC 
CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC 
TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT 
GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA 
GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG 
CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA 
AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT 
GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC 
TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA 
AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT 
GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT 
TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG 
TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC 
CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT 
AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT 
GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA 
TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT 
TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT 
AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT 
AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG 
AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC 
CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC 
GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA 
CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA 
CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA 
AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA 
TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC 
AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT 
ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG 
GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC 
TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG 
ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA 
ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT 
TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT 
GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA 
AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG 
AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA 
GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC 
GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG 
CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG 
CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA 
GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC 
CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT 
CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC 
TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC 
CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA 
ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA 
TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC 
ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA 
CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160768.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160825.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.

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CloneID	ODa56658
Clone ID Related Accession (Same CDS sequence)	XM_005160768.3 , XM_005160768.4
Accession Version	XM_005160768.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160825.3
CDS	5..4618
Translation	MFNVSRPDCVSGYGLAGVGLTSSGVSSGHRARIMSCLEAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160768.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160768.3

ORF Insert Sequence:

ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160768.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160825.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.

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CloneID	ODa56658
Clone ID Related Accession (Same CDS sequence)	XM_005160768.3 , XM_005160768.4
Accession Version	XM_005160768.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160768.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160825.3
CDS	11..4624
Translation	MFNVSRPDCVSGYGLAGVGLTSSGVSSGHRARIMSCLEAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160768.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160768.4

ORF Insert Sequence:

ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160764.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160821.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.

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CloneID	ODa25046
Clone ID Related Accession (Same CDS sequence)	XM_005160764.3 , XM_005160764.4
Accession Version	XM_005160764.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA 
AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG 
GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA 
CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA 
ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC 
CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT 
GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT 
TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA 
CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG 
GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA 
GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG 
CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT 
GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT 
GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA 
GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA 
GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT 
AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG 
ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA 
CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA 
CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG 
TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT 
ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA 
TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC 
TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT 
ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG 
AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT 
GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT 
GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT 
GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT 
CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG 
TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT 
CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA 
CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT 
ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG 
CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT 
TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT 
GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA 
AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT 
CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC 
CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC 
CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT 
TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT 
CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG 
GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA 
CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC 
CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA 
AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA 
AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC 
ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC 
TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC 
TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG 
CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA 
GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT 
ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT 
GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT 
GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT 
GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC 
AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT 
CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG 
CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG 
GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG 
ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT 
CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA 
GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA 
TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC 
ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA 
GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA 
TGTGAGCAGA GCAGCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160821.1
CDS	720..5540
Translation	MPFPERAVEPQLLCRLRGSDGPEKSFITPDGRKVRKPVLFSSLEEVCCHTFTSILHQLSDLSRHASDIFLGIETQAGLISHRTSRIQARLERIQHTVQTLDPKSVKIPVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160764.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160764.3

ORF Insert Sequence:

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA 
AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG 
GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA 
CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA 
ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC 
CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT 
GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT 
TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA 
CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG 
GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA 
GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG 
CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT 
GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT 
GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA 
GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA 
GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT 
AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG 
ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA 
CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA 
CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG 
TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT 
ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA 
TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC 
TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT 
ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG 
AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT 
GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT 
GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT 
GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT 
CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG 
TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT 
CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA 
CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT 
ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG 
CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT 
TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT 
GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA 
AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT 
CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC 
CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC 
CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT 
TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT 
CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG 
GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA 
CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC 
CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA 
AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA 
AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC 
ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC 
TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC 
TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG 
CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA 
GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT 
ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT 
GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT 
GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT 
GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC 
AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT 
CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG 
CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG 
GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG 
ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT 
CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA 
GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA 
TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC 
ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA 
GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA 
TGTGAGCAGA GCAGCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160764.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160821.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.

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CloneID	ODa25046
Clone ID Related Accession (Same CDS sequence)	XM_005160764.3 , XM_005160764.4
Accession Version	XM_005160764.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160764.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA 
AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG 
GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA 
CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA 
ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC 
CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT 
GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT 
TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA 
CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG 
GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA 
GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG 
CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT 
GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT 
GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA 
GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA 
GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT 
AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG 
ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA 
CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA 
CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG 
TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT 
ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA 
TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC 
TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT 
ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG 
AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT 
GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT 
GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT 
GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT 
CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG 
TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT 
CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA 
CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT 
ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG 
CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT 
TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT 
GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA 
AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT 
CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC 
CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC 
CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT 
TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT 
CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG 
GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA 
CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC 
CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA 
AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA 
AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC 
ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC 
TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC 
TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG 
CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA 
GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT 
ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT 
GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT 
GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT 
GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC 
AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT 
CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG 
CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG 
GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG 
ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT 
CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA 
GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA 
TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC 
ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA 
GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA 
TGTGAGCAGA GCAGCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160821.1
CDS	497..5317
Translation	MPFPERAVEPQLLCRLRGSDGPEKSFITPDGRKVRKPVLFSSLEEVCCHTFTSILHQLSDLSRHASDIFLGIETQAGLISHRTSRIQARLERIQHTVQTLDPKSVKIPVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160764.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160764.4

ORF Insert Sequence:

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA 
AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG 
GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA 
CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA 
ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC 
CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT 
GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT 
TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA 
CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG 
GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA 
GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG 
CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT 
GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT 
GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA 
GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA 
GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT 
AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG 
ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA 
CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA 
CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG 
TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT 
ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA 
TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC 
TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT 
ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG 
AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT 
GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT 
GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT 
GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT 
CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG 
TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT 
CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA 
CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT 
ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG 
CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT 
TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT 
GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA 
AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT 
CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC 
CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC 
CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT 
TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT 
CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG 
GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA 
CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC 
CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA 
AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA 
AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC 
ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC 
TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC 
TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG 
CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA 
GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT 
ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT 
GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT 
GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT 
GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC 
AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT 
CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG 
CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG 
GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG 
ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT 
CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA 
GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA 
TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC 
ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA 
GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA 
TGTGAGCAGA GCAGCAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160765.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160822.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa25047
Clone ID Related Accession (Same CDS sequence)	XM_005160765.3 , XM_005160765.4
Accession Version	XM_005160765.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG 
AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA 
GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC 
ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC 
CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT 
CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC 
TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA 
CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA 
GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC 
GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG 
ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG 
ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC 
CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG 
GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA 
CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA 
AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA 
CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA 
AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA 
CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA 
AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA 
CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC 
AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG 
GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT 
ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT 
CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC 
TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA 
GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT 
ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT 
GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT 
AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT 
GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC 
CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG 
CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC 
AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG 
AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC 
AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA 
TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT 
CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT 
CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT 
GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA 
CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA 
GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT 
GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA 
AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG 
AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG 
CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT 
AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT 
CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC 
CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG 
AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG 
ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA 
CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA 
ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA 
GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC 
CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT 
AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT 
TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC 
CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA 
TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA 
AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT 
CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG 
GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA 
ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC 
ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC 
AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC 
ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG 
GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA 
CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG 
GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG 
ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC 
AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC 
AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG 
CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG 
AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT 
GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA 
CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT 
GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC 
ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160822.1
CDS	312..5051
Translation	MGNTPPSQLQSPSGLDPAQTPGVRSDCGIRRRLLASKVHQRPESLWTPKPMLRAEVKGSQGDTLTRSQSCCKRNSLSCFPTVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160765.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160765.3

ORF Insert Sequence:

ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG 
AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA 
GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC 
ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC 
CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT 
CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC 
TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA 
CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA 
GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC 
GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG 
ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG 
ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC 
CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG 
GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA 
CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA 
AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA 
CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA 
AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA 
CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA 
AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA 
CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC 
AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG 
GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT 
ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT 
CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC 
TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA 
GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT 
ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT 
GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT 
AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT 
GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC 
CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG 
CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC 
AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG 
AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC 
AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA 
TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT 
CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT 
CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT 
GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA 
CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA 
GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT 
GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA 
AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG 
AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG 
CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT 
AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT 
CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC 
CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG 
AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG 
ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA 
CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA 
ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA 
GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC 
CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT 
AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT 
TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC 
CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA 
TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA 
AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT 
CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG 
GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA 
ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC 
ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC 
AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC 
ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG 
GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA 
CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG 
GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG 
ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC 
AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC 
AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG 
CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG 
AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT 
GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA 
CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT 
GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC 
ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160765.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160822.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.

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CloneID	ODa25047
Clone ID Related Accession (Same CDS sequence)	XM_005160765.3 , XM_005160765.4
Accession Version	XM_005160765.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160765.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG 
AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA 
GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC 
ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC 
CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT 
CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC 
TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA 
CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA 
GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC 
GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG 
ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG 
ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC 
CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG 
GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA 
CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA 
AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA 
CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA 
AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA 
CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA 
AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA 
CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC 
AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG 
GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT 
ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT 
CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC 
TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA 
GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT 
ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT 
GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT 
AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT 
GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC 
CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG 
CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC 
AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG 
AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC 
AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA 
TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT 
CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT 
CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT 
GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA 
CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA 
GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT 
GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA 
AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG 
AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG 
CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT 
AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT 
CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC 
CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG 
AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG 
ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA 
CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA 
ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA 
GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC 
CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT 
AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT 
TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC 
CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA 
TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA 
AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT 
CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG 
GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA 
ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC 
ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC 
AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC 
ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG 
GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA 
CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG 
GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG 
ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC 
AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC 
AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG 
CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG 
AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT 
GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA 
CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT 
GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC 
ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160822.1
CDS	297..5036
Translation	MGNTPPSQLQSPSGLDPAQTPGVRSDCGIRRRLLASKVHQRPESLWTPKPMLRAEVKGSQGDTLTRSQSCCKRNSLSCFPTVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160765.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160765.4

ORF Insert Sequence:

ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG 
AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA 
GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC 
ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC 
CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT 
CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC 
TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA 
CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA 
GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC 
GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG 
ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG 
ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC 
CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG 
GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA 
CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA 
AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA 
CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA 
AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA 
CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA 
AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA 
CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC 
AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG 
GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT 
ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT 
CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC 
TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA 
GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT 
ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT 
GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT 
AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT 
GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC 
CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG 
CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC 
AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG 
AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC 
AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA 
TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT 
CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT 
CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT 
GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA 
CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA 
GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT 
GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA 
AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG 
AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG 
CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT 
AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT 
CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC 
CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG 
AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG 
ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA 
CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA 
ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA 
GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC 
CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT 
AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT 
TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC 
CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA 
TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA 
AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT 
CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG 
GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA 
ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC 
ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC 
AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC 
ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG 
GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA 
CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG 
GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG 
ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC 
AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC 
AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG 
CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG 
AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT 
GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA 
CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT 
GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC 
ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160766.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160823.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.

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CloneID	ODa25048
Clone ID Related Accession (Same CDS sequence)	XM_005160766.4 , XM_005160766.3
Accession Version	XM_005160766.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160766.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG 
TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA 
GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG 
AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA 
GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA 
GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA 
CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC 
GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA 
ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC 
TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT 
CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC 
CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA 
AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA 
GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC 
ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG 
GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT 
GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG 
AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC 
TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC 
ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA 
GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA 
AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT 
GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT 
TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG 
TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA 
AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG 
ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT 
TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT 
TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC 
ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG 
GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA 
GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC 
AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA 
CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT 
ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC 
CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC 
CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT 
GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC 
ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC 
TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC 
TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT 
AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT 
TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC 
ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG 
CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC 
TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT 
CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT 
CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT 
CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC 
CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT 
AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA 
TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA 
GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT 
GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG 
CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA 
CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT 
TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA 
ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA 
GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG 
GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG 
TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG 
GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT 
TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC 
TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT 
GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT 
GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC 
AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG 
TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT 
CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC 
TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC 
AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC 
ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC 
GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA 
AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT 
TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC 
AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA 
CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC 
TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160823.1
CDS	1469..6184
Translation	MMRDKRSGSFRRDKTEKPAPISRALSWLSVSSLSQQTRKLFRSQNSLHNHSHTPEGGDDEDDNWVYEPQHYIAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160766.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160766.4

ORF Insert Sequence:

ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG 
TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA 
GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG 
AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA 
GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA 
GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA 
CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC 
GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA 
ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC 
TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT 
CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC 
CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA 
AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA 
GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC 
ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG 
GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT 
GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG 
AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC 
TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC 
ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA 
GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA 
AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT 
GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT 
TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG 
TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA 
AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG 
ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT 
TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT 
TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC 
ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG 
GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA 
GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC 
AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA 
CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT 
ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC 
CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC 
CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT 
GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC 
ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC 
TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC 
TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT 
AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT 
TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC 
ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG 
CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC 
TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT 
CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT 
CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT 
CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC 
CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT 
AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA 
TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA 
GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT 
GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG 
CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA 
CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT 
TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA 
ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA 
GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG 
GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG 
TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG 
GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT 
TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC 
TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT 
GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT 
GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC 
AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG 
TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT 
CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC 
TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC 
AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC 
ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC 
GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA 
AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT 
TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC 
AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA 
CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC 
TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160766.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160823.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.

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CloneID	ODa25048
Clone ID Related Accession (Same CDS sequence)	XM_005160766.4 , XM_005160766.3
Accession Version	XM_005160766.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4716bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG 
TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA 
GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG 
AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA 
GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA 
GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA 
CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC 
GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA 
ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC 
TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT 
CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC 
CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA 
AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA 
GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC 
ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG 
GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT 
GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG 
AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC 
TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC 
ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA 
GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA 
AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT 
GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT 
TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG 
TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA 
AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG 
ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT 
TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT 
TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC 
ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG 
GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA 
GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC 
AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA 
CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT 
ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC 
CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC 
CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT 
GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC 
ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC 
TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC 
TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT 
AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT 
TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC 
ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG 
CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC 
TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT 
CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT 
CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT 
CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC 
CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT 
AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA 
TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA 
GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT 
GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG 
CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA 
CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT 
TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA 
ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA 
GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG 
GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG 
TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG 
GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT 
TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC 
TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT 
GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT 
GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC 
AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG 
TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT 
CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC 
TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC 
AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC 
ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC 
GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA 
AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT 
TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC 
AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA 
CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC 
TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160823.1
CDS	1487..6202
Translation	MMRDKRSGSFRRDKTEKPAPISRALSWLSVSSLSQQTRKLFRSQNSLHNHSHTPEGGDDEDDNWVYEPQHYIAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160766.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160766.3

ORF Insert Sequence:

ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG 
TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA 
GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG 
AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA 
GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA 
GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA 
CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC 
GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA 
ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC 
TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT 
CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC 
CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA 
AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA 
GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC 
ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG 
GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT 
GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG 
AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC 
TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC 
ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA 
GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA 
AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT 
GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT 
TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG 
TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA 
AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG 
ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT 
TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT 
TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC 
ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG 
GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA 
GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC 
AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA 
CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT 
ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC 
CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC 
CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT 
GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC 
ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC 
TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC 
TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT 
AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT 
TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC 
ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG 
CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC 
TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT 
CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT 
CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT 
CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC 
CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT 
AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA 
TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA 
GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT 
GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG 
CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA 
CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT 
TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA 
ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA 
GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG 
GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG 
TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG 
GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT 
TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC 
TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT 
GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT 
GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC 
AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG 
TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT 
CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC 
TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC 
AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC 
ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC 
GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA 
AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT 
TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC 
AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA 
CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC 
TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160767.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160824.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25049
Clone ID Related Accession (Same CDS sequence)	XM_005160767.4 , XM_005160767.3
Accession Version	XM_005160767.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160767.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT 
ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA 
TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA 
AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC 
TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA 
ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG 
GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT 
GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG 
AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA 
AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA 
ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC 
AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT 
AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC 
CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC 
AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT 
CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT 
CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG 
GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT 
GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC 
TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT 
TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA 
AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT 
TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA 
GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG 
ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA 
GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC 
CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA 
GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA 
AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT 
GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT 
TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC 
TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG 
TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC 
TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA 
CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC 
ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA 
GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA 
GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT 
CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG 
ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG 
AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT 
TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC 
AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT 
CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC 
CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT 
GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA 
GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG 
AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT 
CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG 
GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC 
ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT 
GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA 
ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC 
AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG 
ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA 
AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG 
GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC 
ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA 
AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC 
ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA 
ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG 
CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC 
CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC 
CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT 
CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC 
AGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160824.1
CDS	496..5184
Translation	MPFPERAVEPQLLCRLRGSDGPEKSFITPDGRKVRKPVLFSSLEEVCCHTFTSILHQLSDLSRHASDIFLGIETQAGLISHRTSRIQARLERIQHTVQTLDPKSVKIPVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160767.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160767.4

ORF Insert Sequence:

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT 
ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA 
TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA 
AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC 
TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA 
ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG 
GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT 
GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG 
AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA 
AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA 
ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC 
AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT 
AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC 
CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC 
AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT 
CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT 
CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG 
GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT 
GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC 
TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT 
TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA 
AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT 
TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA 
GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG 
ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA 
GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC 
CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA 
GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA 
AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT 
GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT 
TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC 
TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG 
TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC 
TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA 
CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC 
ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA 
GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA 
GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT 
CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG 
ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG 
AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT 
TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC 
AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT 
CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC 
CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT 
GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA 
GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG 
AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT 
CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG 
GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC 
ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT 
GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA 
ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC 
AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG 
ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA 
AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG 
GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC 
ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA 
AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC 
ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA 
ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG 
CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC 
CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC 
CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT 
CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC 
AGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_005160767.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160824.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25049
Clone ID Related Accession (Same CDS sequence)	XM_005160767.4 , XM_005160767.3
Accession Version	XM_005160767.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT 
ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA 
TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA 
AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC 
TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA 
ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG 
GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT 
GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG 
AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA 
AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA 
ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC 
AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT 
AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC 
CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC 
AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT 
CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT 
CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG 
GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT 
GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC 
TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT 
TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA 
AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT 
TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA 
GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG 
ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA 
GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC 
CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA 
GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA 
AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT 
GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT 
TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC 
TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG 
TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC 
TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA 
CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC 
ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA 
GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA 
GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT 
CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG 
ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG 
AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT 
TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC 
AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT 
CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC 
CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT 
GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA 
GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG 
AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT 
CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG 
GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC 
ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT 
GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA 
ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC 
AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG 
ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA 
AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG 
GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC 
ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA 
AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC 
ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA 
ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG 
CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC 
CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC 
CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT 
CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC 
AGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160824.1
CDS	720..5408
Translation	MPFPERAVEPQLLCRLRGSDGPEKSFITPDGRKVRKPVLFSSLEEVCCHTFTSILHQLSDLSRHASDIFLGIETQAGLISHRTSRIQARLERIQHTVQTLDPKSVKIPVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_005160767.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160767.3

ORF Insert Sequence:

ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT 
TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT 
CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG 
CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC 
GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG 
CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC 
TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT 
GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC 
TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA 
AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT 
CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC 
CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC 
CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT 
ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA 
TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA 
AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC 
TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA 
ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG 
GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT 
GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG 
AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA 
AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA 
ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC 
AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT 
AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC 
CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC 
AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT 
CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT 
CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG 
GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT 
GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC 
TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT 
TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA 
AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT 
TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA 
GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG 
ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA 
GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC 
CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA 
GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA 
AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT 
GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT 
TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC 
TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG 
TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC 
TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA 
CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC 
ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA 
GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA 
GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT 
CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG 
ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG 
AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT 
TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC 
AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT 
CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC 
CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT 
GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA 
GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG 
AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT 
CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG 
GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC 
ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT 
GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA 
ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC 
AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG 
ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA 
AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG 
GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC 
ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA 
AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC 
ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA 
ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG 
CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC 
CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC 
CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT 
CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC 
AGCAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_003200723.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200771.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.

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CloneID	ODa25051
Clone ID Related Accession (Same CDS sequence)	XM_003200723.4 , XM_003200723.5
Accession Version	XM_003200723.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NHS-like protein 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200723.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200771.1
CDS	159..4712
Translation	MVFIGTSLKTVIKYFKRKAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_003200723.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200723.4

ORF Insert Sequence:

ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsl1b ( XM_003200723.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200771.1 Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.

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CloneID	ODa25051
Clone ID Related Accession (Same CDS sequence)	XM_003200723.4 , XM_003200723.5
Accession Version	XM_003200723.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200723.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200771.1
CDS	168..4721
Translation	MVFIGTSLKTVIKYFKRKAVSNLDEEKKWTVHYTAPWHQQENVFLPGSRPACVEDLHRQAKVNLKTALRECDKLRKDGFRSSQYYSQGPTFSGSSSSQHEDEDLEGDDADKKSTASSGEEEKDSCQMRAQSPAQVEGVEVDGQMSSSKASVLPTPEEKMRQQAQAVLTDIVPINVTGETFDRQASIRRSLINTDTLVRRPKKVKRRKTISGVPDSVQQELAAKGRGGELRPQSMFIPGQYSTLGRVMNGNSTLRQSVTKDSGCQTEAVKIVPPSVRRIRAQKGQGIAAQMAGISTSATNISSVSDGSSTGGSIVAMAPQFGNDIQRFHSLPRGARVSLNAEPIYSSTPFRKEDSCAKAPKQIGKLQVDNTAVHMRNAPRVCTQARPKSQEVRGSQREWGSVSGPACVVSPHAAYSTSLIPNATLSCSAEVFALHSKSSPGQSPLSGSPYTKARPLSMVSVVNSGSTSSVGTGSHTPDAGMKDTCSETGQSDSSLHSHSTIAAGTLSSDEQWIYDTPDNALPRRTMTSSCSTPINHLYSSLERSSKGTDSSSLYSMDNDGYYTSMHLDSGLRSRSHGSGHGHGIGGRAVRHSMYECLGEQEDRNSLCSDHSLSRSISLRKPKKPPLPPARTDSLRRKPGKNSSPSANIGQSNIRNGSLLNESLIATLQQSLQNGLKGKGSSTSPSHSPCSDYEDPWMLRPRSQSSISASSSGVSATGMAHVYSICPVTPSHSDTSSLRSDYADSWGYYMEYPRPSGDQTQSPCTNSHSAGQVSEMANGRGPLNGNQANLPAVQEGSDMSAKPRTGTSSPDRVHRLTSPSSGYSSQSNTPTAGTPVPSFMRCMSPSGKPKPRVPERKSSLLSSVSISSSSTSLSSNTSDSNRSNIPPPPPLPAAPVVLVPLNPPSFPPPLPPPSPVCTEDQKFPPPPPPLPTTPHQQASMNPLQNNSSPEFPPPPPPEVLTDPACSGFNSSFSPPPPPPPPPLPTYSPSQNQHLPHMTPQTPSSASLKEIKGCLKPVNLEGRPVLPQSEEPNKVSMPLITPFALQSVQLRPAKRPENNEVSRTDRPQTPEKTHPTVPQISCISPNVSLSSTEEKPSIQHSQDGLVEAQTDYSVKSNSDPTSFLTNGPIDLGEATDEVDGLKTFLSTQVEKSLNSTPKKKPPMISKKPKFSLTFSSVDHVRDFVSAEVLDPIPVLEKEEETTEKSSFDISVPPVEIRDISPTNGESQEFSQSSGTTILDVDKSTSEAEDEEERDDEDVTSSTGSFSSKDDENGDVFESSTLDVAQSPSINGDSCGDMVTPTRPRTTEDLFAAIHRSKRKVLGRKESEEDRSRGPLSPPVTPTAQSPSLTSSLPRQSGSIQRSLRKSATSSDTFKALLLKKGSRSESSFRMSATEMLRSTDPRFQRTRSLDSSFDPASPTSESPCSSPGRSKRVADDWARNDGMFSTSPSLIGPKYGRSRTPPSAASSKYNARCRILSSPMTVICERDGELTDCEDPVSPSNTPLPISQDSNSTLCEQSSS

Target ORF information:

RefSeq Version	XM_003200723.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200723.5

ORF Insert Sequence:

ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG 
GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC 
AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT 
AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG 
GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA 
GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT 
GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG 
CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT 
GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC 
AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA 
GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT 
TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT 
TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT 
CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC 
TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC 
GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA 
GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG 
CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG 
TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT 
GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT 
AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC 
CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT 
GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG 
AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT 
GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG 
CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT 
GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC 
TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT 
CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG 
GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT 
AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC 
AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA 
TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC 
ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG 
AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA 
TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC 
GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA 
CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT 
CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG 
CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA 
TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC 
ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA 
TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG 
AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT 
CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA 
TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT 
CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC 
CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA 
CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT 
TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA 
CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT 
GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG 
ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA 
TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT 
GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC 
CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT 
TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG 
ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT 
GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG 
AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT 
GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC 
ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA 
TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG 
GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC 
CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC 
AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA 
CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC 
CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC 
TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT 
CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC 
TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG 
TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT 
GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC 
TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC 
CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nhsl1b
Entrez Gene ID	447799
Full Name	NHS-like 1b
Synonyms	gukh2,nhsl1,si:ch211-195e3.6
General protein information	Preferred Names NHS-like 1b Names NHS-like protein 1 CH211-195E3.6 GUK-holder 2 NHS-like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	NHSL1	XP_001171693.2
Mus musculus (house mouse)	Nhsl1	NP_775566.3
Danio rerio (zebrafish)	nhsl1b	XP_005160821.1
Canis lupus familiaris (dog)	NHSL1	XP_005615510.1
Rattus norvegicus (Norway rat)	Nhsl1	XP_006227729.1
	NHSL1	XP_001095123.2
Xenopus tropicalis (tropical clawed frog)	nhsl1	NP_001135597.1
Homo sapiens (human)	NHSL1	NP_065197.1
Bos taurus (cattle)	NHSL1	NP_001178467.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nhsl1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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