nhsl1b cDNA ORF clone, Danio rerio(zebrafish)

The following nhsl1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhsl1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa41206 XM_009294777.3
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $881.30
$1259.00
ODa56658 XM_005160768.4
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa25046 XM_005160764.4
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25047 XM_005160765.4
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25048 XM_005160766.4
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25049 XM_005160767.4
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25051 XM_003200723.5
Latest version!
Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
ODa41206 XM_009294777.2 Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $881.30
$1259.00
ODa25046 XM_005160764.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25047 XM_005160765.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25048 XM_005160766.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa25049 XM_005160767.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa56658 XM_005160768.3 Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa25051 XM_003200723.4 Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa41206
    Clone ID Related Accession (Same CDS sequence) XM_009294777.3 , XM_009294777.2
    Accession Version XM_009294777.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294777.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
    GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG
    CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG
    GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC
    CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT
    GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG
    GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG
    GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG
    AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG
    TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA
    CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA
    CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG
    TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA
    GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG
    GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC
    ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG
    TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT
    GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT
    AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG
    GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT
    TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA
    CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT
    GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA
    GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC
    ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC
    ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC
    TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC
    CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC
    TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT
    GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA
    GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG
    CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA
    AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT
    GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC
    TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA
    AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT
    GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT
    TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG
    TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC
    CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT
    AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT
    GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA
    TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT
    TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT
    AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT
    AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG
    AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC
    CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC
    GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA
    CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA
    CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA
    AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA
    TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC
    AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT
    ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG
    GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC
    TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG
    ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA
    ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT
    TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT
    GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA
    AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG
    AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA
    GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC
    GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG
    CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG
    CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA
    GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC
    CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT
    CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC
    TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC
    CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA
    ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA
    TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC
    ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA
    CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293052.1
    CDS32..4588
    Translation

    Target ORF information:

    RefSeq Version XM_009294777.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294777.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
    GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG
    CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG
    GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC
    CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT
    GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG
    GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG
    GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG
    AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG
    TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA
    CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA
    CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG
    TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA
    GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG
    GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC
    ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG
    TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT
    GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT
    AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG
    GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT
    TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA
    CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT
    GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA
    GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC
    ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC
    ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC
    TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC
    CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC
    TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT
    GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA
    GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG
    CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA
    AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT
    GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC
    TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA
    AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT
    GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT
    TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG
    TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC
    CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT
    AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT
    GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA
    TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT
    TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT
    AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT
    AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG
    AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC
    CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC
    GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA
    CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA
    CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA
    AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA
    TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC
    AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT
    ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG
    GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC
    TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG
    ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA
    ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT
    TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT
    GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA
    AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG
    AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA
    GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC
    GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG
    CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG
    CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA
    GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC
    CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT
    CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC
    TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC
    CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA
    ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA
    TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC
    ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA
    CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa41206
    Clone ID Related Accession (Same CDS sequence) XM_009294777.3 , XM_009294777.2
    Accession Version XM_009294777.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4557bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
    GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG
    CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG
    GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC
    CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT
    GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG
    GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG
    GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG
    AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG
    TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA
    CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA
    CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG
    TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA
    GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG
    GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC
    ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG
    TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT
    GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT
    AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG
    GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT
    TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA
    CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT
    GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA
    GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC
    ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC
    ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC
    TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC
    CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC
    TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT
    GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA
    GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG
    CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA
    AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT
    GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC
    TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA
    AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT
    GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT
    TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG
    TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC
    CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT
    AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT
    GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA
    TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT
    TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT
    AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT
    AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG
    AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC
    CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC
    GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA
    CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA
    CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA
    AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA
    TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC
    AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT
    ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG
    GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC
    TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG
    ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA
    ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT
    TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT
    GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA
    AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG
    AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA
    GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC
    GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG
    CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG
    CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA
    GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC
    CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT
    CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC
    TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC
    CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA
    ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA
    TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC
    ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA
    CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009293052.1
    CDS16..4572
    Translation

    Target ORF information:

    RefSeq Version XM_009294777.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009294777.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCCTGTCA AAGGGATCAG TTTCTGGGGC CACAGGAAGA GGAAAAGCCC CTACTCAGCT 
    GTATCTAATC TGGATGAGGA GAAAAAGTGG ACCGTGCACT ACACTGCCCC ATGGCACCAG
    CAGGAGAACG TCTTTCTGCC AGGCTCCAGA CCGGCCTGTG TGGAAGATCT TCACCGTCAG
    GCCAAAGTGA ACCTGAAAAC AGCTCTCAGA GAATGTGATA AACTGAGGAA GGATGGCTTC
    CGTAGCTCGC AGTACTACTC ACAGGGGCCC ACTTTCTCTG GCTCCTCAAG CTCCCAACAT
    GAGGACGAGG ACCTTGAAGG CGATGACGCT GACAAAAAGT CCACAGCATC TTCAGGAGAG
    GAAGAGAAAG ACTCCTGCCA AATGAGAGCG CAAAGTCCGG CACAGGTGGA AGGAGTCGAG
    GTTGATGGAC AAATGTCGAG CTCCAAAGCC TCAGTCCTGC CCACTCCAGA GGAGAAGATG
    AGGCAACAGG CACAGGCCGT TCTCACAGAT ATTGTCCCCA TTAATGTCAC GGGAGAGACG
    TTTGACAGGC AGGCCAGTAT CCGCCGCTCC CTAATAAACA CTGACACACT GGTCCGCCGA
    CCCAAGAAGG TTAAACGGAG AAAGACTATT TCAGGGGTGC CTGACAGCGT CCAACAGGAA
    CTAGCGGCTA AAGGGCGTGG AGGAGAGCTT CGACCACAGT CCATGTTTAT CCCTGGACAG
    TATTCAACAC TTGGGCGAGT CATGAATGGG AATTCAACTC TGCGTCAATC TGTAACAAAA
    GATTCTGGCT GCCAGACTGA GGCGGTGAAA ATCGTCCCAC CCTCAGTAAG GAGAATACGG
    GCTCAGAAGG GTCAAGGAAT TGCTGCTCAG ATGGCTGGCA TCTCCACCTC AGCCACAAAC
    ATCTCTTCTG TTTCTGATGG GAGCTCCACT GGAGGTTCGA TTGTTGCAAT GGCACCACAG
    TTCGGCAATG ATATCCAACG CTTTCACAGC TTGCCACGGG GTGCACGGGT CTCTCTAAAT
    GCAGAGCCCA TTTACAGTAG CACCCCTTTC AGAAAGGAAG ACTCCTGTGC AAAAGCACCT
    AAGCAGATTG GAAAATTACA AGTAGACAAT ACTGCAGTGC ACATGAGGAA TGCCCCCAGG
    GTGTGTACTC AAGCCCGCCC AAAATCTCAG GAGGTGCGAG GATCTCAAAG GGAGTGGGGT
    TCTGTCTCGG GTCCAGCCTG TGTAGTTTCT CCACATGCAG CCTACTCAAC CTCCCTTATA
    CCTAATGCTA CTCTGTCATG TTCTGCTGAG GTTTTTGCTC TTCACAGCAA ATCAAGTCCT
    GGCCAGAGCC CACTTTCTGG GTCACCATAC ACTAAAGCTA GACCTCTTAG CATGGTCTCA
    GTTGTCAACA GTGGGAGTAC AAGTAGTGTG GGCACAGGAT CTCACACCCC AGATGCAGGC
    ATGAAAGATA CCTGCAGTGA GACTGGCCAA TCTGATAGCA GTTTGCACAG CCACAGTACC
    ATTGCCGCAG GAACATTGTC TTCAGATGAG CAGTGGATTT ACGATACTCC TGATAATGCC
    TTGCCCAGAA GGACAATGAC TTCCAGCTGC TCAACACCCA TAAATCATCT ATATAGTAGC
    CTTGAACGCT CCTCAAAAGG CACAGACTCT AGTTCACTGT ACTCTATGGA CAATGATGGC
    TACTACACTT CCATGCATCT GGATTCAGGC CTTAGGTCAA GAAGCCATGG TAGCGGCCAT
    GGTCATGGCA TAGGAGGAAG AGCAGTAAGA CACAGTATGT ACGAGTGCTT GGGTGAGCAA
    GAGGATCGAA ATAGCTTGTG CAGCGACCAC TCGCTATCTC GTTCCATTTC TCTACGCAAG
    CCTAAGAAAC CACCCCTTCC ACCTGCACGC ACAGACTCTC TACGGAGAAA GCCTGGGAAA
    AACAGCTCTC CCTCTGCCAA TATTGGGCAG TCAAATATTA GGAATGGTTC TCTTCTCAAT
    GAATCATTGA TTGCCACTCT CCAACAGTCA CTTCAGAATG GGCTTAAAGG CAAAGGATCC
    TCCACGTCAC CATCTCATAG CCCTTGTAGT GACTATGAGG ACCCCTGGAT GCTTCGTCCA
    AGGAGCCAGA GCAGCATCAG TGCAAGTAGT AGTGGTGTAT CAGCTACAGG AATGGCTCAT
    GTATACTCCA TCTGTCCCGT CACACCATCT CATAGTGACA CAAGTAGCCT CCGTTCTGAT
    TACGCCGACT CCTGGGGCTA CTACATGGAA TACCCTCGTC CATCTGGAGA TCAGACACAG
    TCACCATGTA CCAATTCACA CTCTGCTGGA CAAGTGTCTG AGATGGCAAA TGGAAGAGGC
    CCTCTCAATG GTAACCAGGC TAATCTTCCT GCTGTTCAGG AAGGAAGTGA CATGTCTGCT
    AAGCCCAGGA CAGGCACCTC TTCACCGGAC AGGGTACATC GGTTGACTTC TCCATCAAGT
    GGATACTCAA GTCAGTCAAA CACTCCAACT GCTGGTACTC CCGTTCCCTC CTTCATGAGA
    TGCATGTCCC CATCAGGCAA GCCCAAACCA AGAGTGCCTG AAAGAAAGTC ATCCTTGCTT
    TCATCTGTAT CTATATCCTC CTCTTCCACC TCTCTTTCAT CCAACACCTC TGATTCTAAT
    AGGAGCAATA TTCCACCTCC TCCTCCTCTT CCAGCTGCCC CTGTGGTACT TGTACCTCTT
    AATCCTCCAT CCTTTCCTCC TCCCCTTCCA CCCCCAAGCC CTGTGTGCAC TGAAGACCAG
    AAATTTCCAC CTCCGCCACC TCCTTTACCC ACAACTCCCC ATCAGCAAGC ATCCATGAAC
    CCTCTTCAAA ACAATTCCTC CCCTGAATTT CCACCCCCTC CACCTCCAGA AGTGTTGACC
    GACCCTGCCT GTTCAGGCTT TAATAGTTCC TTCAGCCCTC CACCACCTCC CCCTCCACCA
    CCACTGCCTA CTTATTCTCC ATCCCAAAAT CAACATTTAC CCCACATGAC GCCACAAACA
    CCTTCTTCTG CCTCCTTGAA AGAAATTAAG GGCTGTCTTA AACCAGTGAA CTTAGAAGGA
    AGACCAGTGT TGCCTCAATC TGAGGAACCT AATAAGGTTA GCATGCCTTT GATTACCCCA
    TTTGCACTGC AGAGTGTGCA GCTTCGGCCA GCAAAACGTC CAGAAAACAA TGAAGTTAGC
    AGGACAGACA GACCCCAAAC ACCAGAAAAA ACTCACCCCA CAGTTCCTCA GATTTCTTGT
    ATATCTCCAA ATGTAAGCCT TTCATCTACA GAGGAAAAAC CCTCAATCCA GCATAGCCAG
    GATGGCCTTG TTGAAGCACA AACTGACTAT AGTGTAAAAT CCAATTCTGA TCCAACATCC
    TTCCTTACAA ATGGGCCCAT AGATCTTGGT GAGGCCACAG ATGAAGTAGA TGGTTTAAAG
    ACTTTCCTCT CAACACAAGT GGAAAAGTCA CTAAACTCTA CACCCAAGAA AAAGCCTCCA
    ATGATTTCCA AAAAGCCCAA GTTTTCTCTT ACTTTCTCCT CTGTGGATCA TGTAAGGGAT
    TTTGTGAGTG CTGAAGTTCT GGACCCCATA CCTGTGCTGG AAAAAGAGGA AGAGACAACT
    GAGAAAAGTA GCTTTGACAT TTCCGTACCT CCTGTAGAGA TAAGAGACAT TTCTCCCACA
    AATGGTGAAT CTCAGGAGTT CTCACAATCC TCTGGTACCA CTATTCTTGA TGTAGACAAG
    AGCACCTCTG AAGCAGAGGA TGAGGAAGAA AGAGATGACG AAGATGTAAC CAGCAGCACA
    GGATCGTTCA GTTCTAAAGA TGATGAAAAT GGTGATGTGT TTGAATCCAG CACGTTGGAC
    GTGGCTCAAT CACCCAGCAT CAACGGTGAC AGCTGTGGGG ACATGGTGAC CCCCACACGG
    CCCCGCACCA CAGAGGACCT GTTTGCAGCC ATTCACAGGT CGAAGAGAAA AGTCCTGGGG
    CGCAAGGAAT CAGAAGAGGA TCGCTCCCGG GGTCCTCTAT CTCCACCGGT CACCCCGACA
    GCACAGAGCC CCAGCTTGAC CTCCTCTCTG CCCCGGCAAT CAGGCTCCAT CCAGCGCAGC
    CTCCGCAAAT CTGCAACCAG CAGTGACACC TTCAAGGCCC TCCTGCTAAA GAAAGGCAGT
    CGCTCTGAAA GCAGTTTCCG CATGTCTGCA ACCGAGATGC TGCGCAGCAC CGACCCGCGC
    TTTCAACGCA CCCGCTCCCT CGACTCCTCC TTTGATCCGG CGTCTCCAAC ATCCGAGAGC
    CCCTGCTCTT CTCCAGGCCG AAGCAAGAGG GTGGCAGATG ACTGGGCGCG CAATGATGGA
    ATGTTTTCAA CTTCGCCATC TTTGATAGGC CCAAAATACG GACGATCCCG AACGCCACCA
    TCTGCTGCTA GCAGCAAATA CAACGCCCGC TGCCGCATCC TCAGCAGCCC CATGACTGTC
    ATCTGCGAGA GGGATGGAGA ACTAACCGAC TGTGAAGACC CCGTTTCTCC GTCAAACACA
    CCCCTTCCCA TCTCTCAGGA CTCAAACAGC ACTTTATGTG AGCAGAGCAG CAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56658
    Clone ID Related Accession (Same CDS sequence) XM_005160768.3 , XM_005160768.4
    Accession Version XM_005160768.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
    ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160825.3
    CDS5..4618
    Translation

    Target ORF information:

    RefSeq Version XM_005160768.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160768.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
    ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56658
    Clone ID Related Accession (Same CDS sequence) XM_005160768.3 , XM_005160768.4
    Accession Version XM_005160768.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4614bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160768.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
    ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160825.3
    CDS11..4624
    Translation

    Target ORF information:

    RefSeq Version XM_005160768.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160768.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGTTCAACG TGAGCAGGCC TGACTGTGTG TCTGGATATG GGCTCGCTGG AGTGGGATTG 
    ACATCTTCAG GGGTCTCTAG TGGACACAGG GCTAGAATCA TGTCATGCCT TGAAGCTGTA
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25046
    Clone ID Related Accession (Same CDS sequence) XM_005160764.3 , XM_005160764.4
    Accession Version XM_005160764.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA
    AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG
    GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA
    CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA
    ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC
    CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT
    GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT
    TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA
    CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG
    GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA
    GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG
    CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT
    GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT
    GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA
    GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA
    GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT
    AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG
    ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA
    CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA
    CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG
    TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT
    ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA
    TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC
    TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT
    ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG
    AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT
    GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT
    GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT
    GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT
    CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG
    TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT
    CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA
    CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT
    ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG
    CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT
    TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT
    GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA
    AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT
    CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC
    CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC
    CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT
    TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT
    CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG
    GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA
    CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC
    CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA
    AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA
    AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC
    ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC
    TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC
    TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG
    CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA
    GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT
    ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT
    GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT
    GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT
    GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC
    AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT
    CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG
    CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG
    GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG
    ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT
    CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA
    GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA
    TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC
    ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA
    GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA
    TGTGAGCAGA GCAGCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160821.1
    CDS720..5540
    Translation

    Target ORF information:

    RefSeq Version XM_005160764.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160764.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA
    AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG
    GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA
    CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA
    ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC
    CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT
    GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT
    TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA
    CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG
    GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA
    GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG
    CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT
    GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT
    GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA
    GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA
    GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT
    AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG
    ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA
    CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA
    CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG
    TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT
    ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA
    TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC
    TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT
    ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG
    AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT
    GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT
    GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT
    GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT
    CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG
    TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT
    CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA
    CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT
    ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG
    CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT
    TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT
    GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA
    AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT
    CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC
    CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC
    CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT
    TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT
    CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG
    GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA
    CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC
    CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA
    AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA
    AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC
    ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC
    TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC
    TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG
    CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA
    GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT
    ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT
    GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT
    GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT
    GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC
    AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT
    CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG
    CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG
    GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG
    ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT
    CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA
    GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA
    TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC
    ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA
    GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA
    TGTGAGCAGA GCAGCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25046
    Clone ID Related Accession (Same CDS sequence) XM_005160764.3 , XM_005160764.4
    Accession Version XM_005160764.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4821bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160764.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA
    AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG
    GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA
    CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA
    ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC
    CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT
    GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT
    TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA
    CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG
    GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA
    GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG
    CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT
    GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT
    GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA
    GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA
    GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT
    AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG
    ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA
    CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA
    CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG
    TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT
    ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA
    TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC
    TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT
    ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG
    AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT
    GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT
    GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT
    GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT
    CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG
    TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT
    CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA
    CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT
    ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG
    CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT
    TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT
    GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA
    AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT
    CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC
    CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC
    CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT
    TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT
    CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG
    GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA
    CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC
    CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA
    AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA
    AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC
    ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC
    TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC
    TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG
    CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA
    GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT
    ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT
    GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT
    GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT
    GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC
    AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT
    CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG
    CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG
    GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG
    ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT
    CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA
    GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA
    TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC
    ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA
    GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA
    TGTGAGCAGA GCAGCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160821.1
    CDS497..5317
    Translation

    Target ORF information:

    RefSeq Version XM_005160764.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160764.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGGAGA GACGTTTGAC AGGCAGGCCA GTATCCGCCG CTCCCTAATA
    AACACTGACA CACTGGTCCG CCGACCCAAG AAGGTTAAAC GGAGAAAGAC TATTTCAGGG
    GTGCCTGACA GCGTCCAACA GGAACTAGCG GCTAAAGGGC GTGGAGGAGA GCTTCGACCA
    CAGTCCATGT TTATCCCTGG ACAGTATTCA ACACTTGGGC GAGTCATGAA TGGGAATTCA
    ACTCTGCGTC AATCTGTAAC AAAAGATTCT GGCTGCCAGA CTGAGGCGGT GAAAATCGTC
    CCACCCTCAG TAAGGAGAAT ACGGGCTCAG AAGGGTCAAG GAATTGCTGC TCAGATGGCT
    GGCATCTCCA CCTCAGCCAC AAACATCTCT TCTGTTTCTG ATGGGAGCTC CACTGGAGGT
    TCGATTGTTG CAATGGCACC ACAGTTCGGC AATGATATCC AACGCTTTCA CAGCTTGCCA
    CGGGGTGCAC GGGTCTCTCT AAATGCAGAG CCCATTTACA GTAGCACCCC TTTCAGAAAG
    GAAGACTCCT GTGCAAAAGC ACCTAAGCAG ATTGGAAAAT TACAAGTAGA CAATACTGCA
    GTGCACATGA GGAATGCCCC CAGGGTGTGT ACTCAAGCCC GCCCAAAATC TCAGGAGGTG
    CGAGGATCTC AAAGGGAGTG GGGTTCTGTC TCGGGTCCAG CCTGTGTAGT TTCTCCACAT
    GCAGCCTACT CAACCTCCCT TATACCTAAT GCTACTCTGT CATGTTCTGC TGAGGTTTTT
    GCTCTTCACA GCAAATCAAG TCCTGGCCAG AGCCCACTTT CTGGGTCACC ATACACTAAA
    GCTAGACCTC TTAGCATGGT CTCAGTTGTC AACAGTGGGA GTACAAGTAG TGTGGGCACA
    GGATCTCACA CCCCAGATGC AGGCATGAAA GATACCTGCA GTGAGACTGG CCAATCTGAT
    AGCAGTTTGC ACAGCCACAG TACCATTGCC GCAGGAACAT TGTCTTCAGA TGAGCAGTGG
    ATTTACGATA CTCCTGATAA TGCCTTGCCC AGAAGGACAA TGACTTCCAG CTGCTCAACA
    CCCATAAATC ATCTATATAG TAGCCTTGAA CGCTCCTCAA AAGGCACAGA CTCTAGTTCA
    CTGTACTCTA TGGACAATGA TGGCTACTAC ACTTCCATGC ATCTGGATTC AGGCCTTAGG
    TCAAGAAGCC ATGGTAGCGG CCATGGTCAT GGCATAGGAG GAAGAGCAGT AAGACACAGT
    ATGTACGAGT GCTTGGGTGA GCAAGAGGAT CGAAATAGCT TGTGCAGCGA CCACTCGCTA
    TCTCGTTCCA TTTCTCTACG CAAGCCTAAG AAACCACCCC TTCCACCTGC ACGCACAGAC
    TCTCTACGGA GAAAGCCTGG GAAAAACAGC TCTCCCTCTG CCAATATTGG GCAGTCAAAT
    ATTAGGAATG GTTCTCTTCT CAATGAATCA TTGATTGCCA CTCTCCAACA GTCACTTCAG
    AATGGGCTTA AAGGCAAAGG ATCCTCCACG TCACCATCTC ATAGCCCTTG TAGTGACTAT
    GAGGACCCCT GGATGCTTCG TCCAAGGAGC CAGAGCAGCA TCAGTGCAAG TAGTAGTGGT
    GTATCAGCTA CAGGAATGGC TCATGTATAC TCCATCTGTC CCGTCACACC ATCTCATAGT
    GACACAAGTA GCCTCCGTTC TGATTACGCC GACTCCTGGG GCTACTACAT GGAATACCCT
    CGTCCATCTG GAGATCAGAC ACAGTCACCA TGTACCAATT CACACTCTGC TGGACAAGTG
    TCTGAGATGG CAAATGGAAG AGGCCCTCTC AATGGTAACC AGGCTAATCT TCCTGCTGTT
    CAGGAAGGAA GTGACATGTC TGCTAAGCCC AGGACAGGCA CCTCTTCACC GGACAGGGTA
    CATCGGTTGA CTTCTCCATC AAGTGGATAC TCAAGTCAGT CAAACACTCC AACTGCTGGT
    ACTCCCGTTC CCTCCTTCAT GAGATGCATG TCCCCATCAG GCAAGCCCAA ACCAAGAGTG
    CCTGAAAGAA AGTCATCCTT GCTTTCATCT GTATCTATAT CCTCCTCTTC CACCTCTCTT
    TCATCCAACA CCTCTGATTC TAATAGGAGC AATATTCCAC CTCCTCCTCC TCTTCCAGCT
    GCCCCTGTGG TACTTGTACC TCTTAATCCT CCATCCTTTC CTCCTCCCCT TCCACCCCCA
    AGCCCTGTGT GCACTGAAGA CCAGAAATTT CCACCTCCGC CACCTCCTTT ACCCACAACT
    CCCCATCAGC AAGCATCCAT GAACCCTCTT CAAAACAATT CCTCCCCTGA ATTTCCACCC
    CCTCCACCTC CAGAAGTGTT GACCGACCCT GCCTGTTCAG GCTTTAATAG TTCCTTCAGC
    CCTCCACCAC CTCCCCCTCC ACCACCACTG CCTACTTATT CTCCATCCCA AAATCAACAT
    TTACCCCACA TGACGCCACA AACACCTTCT TCTGCCTCCT TGAAAGAAAT TAAGGGCTGT
    CTTAAACCAG TGAACTTAGA AGGAAGACCA GTGTTGCCTC AATCTGAGGA ACCTAATAAG
    GTTAGCATGC CTTTGATTAC CCCATTTGCA CTGCAGAGTG TGCAGCTTCG GCCAGCAAAA
    CGTCCAGAAA ACAATGAAGT TAGCAGGACA GACAGACCCC AAACACCAGA AAAAACTCAC
    CCCACAGTTC CTCAGATTTC TTGTATATCT CCAAATGTAA GCCTTTCATC TACAGAGGAA
    AAACCCTCAA TCCAGCATAG CCAGGATGGC CTTGTTGAAG CACAAACTGA CTATAGTGTA
    AAATCCAATT CTGATCCAAC ATCCTTCCTT ACAAATGGGC CCATAGATCT TGGTGAGGCC
    ACAGATGAAG TAGATGGTTT AAAGACTTTC CTCTCAACAC AAGTGGAAAA GTCACTAAAC
    TCTACACCCA AGAAAAAGCC TCCAATGATT TCCAAAAAGC CCAAGTTTTC TCTTACTTTC
    TCCTCTGTGG ATCATGTAAG GGATTTTGTG AGTGCTGAAG TTCTGGACCC CATACCTGTG
    CTGGAAAAAG AGGAAGAGAC AACTGAGAAA AGTAGCTTTG ACATTTCCGT ACCTCCTGTA
    GAGATAAGAG ACATTTCTCC CACAAATGGT GAATCTCAGG AGTTCTCACA ATCCTCTGGT
    ACCACTATTC TTGATGTAGA CAAGAGCACC TCTGAAGCAG AGGATGAGGA AGAAAGAGAT
    GACGAAGATG TAACCAGCAG CACAGGATCG TTCAGTTCTA AAGATGATGA AAATGGTGAT
    GTGTTTGAAT CCAGCACGTT GGACGTGGCT CAATCACCCA GCATCAACGG TGACAGCTGT
    GGGGACATGG TGACCCCCAC ACGGCCCCGC ACCACAGAGG ACCTGTTTGC AGCCATTCAC
    AGGTCGAAGA GAAAAGTCCT GGGGCGCAAG GAATCAGAAG AGGATCGCTC CCGGGGTCCT
    CTATCTCCAC CGGTCACCCC GACAGCACAG AGCCCCAGCT TGACCTCCTC TCTGCCCCGG
    CAATCAGGCT CCATCCAGCG CAGCCTCCGC AAATCTGCAA CCAGCAGTGA CACCTTCAAG
    GCCCTCCTGC TAAAGAAAGG CAGTCGCTCT GAAAGCAGTT TCCGCATGTC TGCAACCGAG
    ATGCTGCGCA GCACCGACCC GCGCTTTCAA CGCACCCGCT CCCTCGACTC CTCCTTTGAT
    CCGGCGTCTC CAACATCCGA GAGCCCCTGC TCTTCTCCAG GCCGAAGCAA GAGGGTGGCA
    GATGACTGGG CGCGCAATGA TGGAATGTTT TCAACTTCGC CATCTTTGAT AGGCCCAAAA
    TACGGACGAT CCCGAACGCC ACCATCTGCT GCTAGCAGCA AATACAACGC CCGCTGCCGC
    ATCCTCAGCA GCCCCATGAC TGTCATCTGC GAGAGGGATG GAGAACTAAC CGACTGTGAA
    GACCCCGTTT CTCCGTCAAA CACACCCCTT CCCATCTCTC AGGACTCAAA CAGCACTTTA
    TGTGAGCAGA GCAGCAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25047
    Clone ID Related Accession (Same CDS sequence) XM_005160765.3 , XM_005160765.4
    Accession Version XM_005160765.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160765.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
    CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG
    AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA
    GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC
    ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC
    CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT
    CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC
    TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA
    CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA
    GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC
    GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG
    ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG
    ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC
    CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG
    GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA
    CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA
    AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA
    CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA
    AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA
    CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA
    AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA
    CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC
    AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG
    GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT
    ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT
    CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC
    TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA
    GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT
    ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT
    GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT
    AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT
    GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC
    CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG
    CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC
    AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG
    AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC
    AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA
    TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT
    CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT
    CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT
    GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA
    CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA
    GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT
    GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA
    AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG
    AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG
    CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT
    AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT
    CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC
    CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG
    AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG
    ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA
    CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA
    ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA
    GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC
    CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT
    AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT
    TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC
    CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA
    TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA
    AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT
    CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG
    GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA
    ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC
    ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC
    AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC
    ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG
    GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA
    CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG
    GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG
    ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC
    AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC
    AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG
    CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG
    AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT
    GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA
    CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT
    GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC
    ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160822.1
    CDS312..5051
    Translation

    Target ORF information:

    RefSeq Version XM_005160765.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160765.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
    CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG
    AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA
    GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC
    ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC
    CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT
    CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC
    TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA
    CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA
    GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC
    GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG
    ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG
    ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC
    CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG
    GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA
    CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA
    AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA
    CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA
    AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA
    CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA
    AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA
    CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC
    AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG
    GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT
    ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT
    CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC
    TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA
    GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT
    ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT
    GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT
    AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT
    GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC
    CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG
    CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC
    AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG
    AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC
    AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA
    TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT
    CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT
    CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT
    GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA
    CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA
    GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT
    GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA
    AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG
    AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG
    CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT
    AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT
    CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC
    CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG
    AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG
    ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA
    CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA
    ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA
    GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC
    CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT
    AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT
    TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC
    CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA
    TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA
    AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT
    CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG
    GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA
    ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC
    ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC
    AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC
    ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG
    GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA
    CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG
    GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG
    ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC
    AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC
    AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG
    CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG
    AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT
    GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA
    CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT
    GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC
    ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25047
    Clone ID Related Accession (Same CDS sequence) XM_005160765.3 , XM_005160765.4
    Accession Version XM_005160765.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160765.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
    CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG
    AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA
    GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC
    ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC
    CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT
    CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC
    TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA
    CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA
    GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC
    GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG
    ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG
    ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC
    CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG
    GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA
    CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA
    AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA
    CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA
    AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA
    CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA
    AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA
    CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC
    AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG
    GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT
    ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT
    CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC
    TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA
    GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT
    ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT
    GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT
    AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT
    GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC
    CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG
    CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC
    AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG
    AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC
    AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA
    TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT
    CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT
    CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT
    GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA
    CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA
    GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT
    GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA
    AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG
    AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG
    CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT
    AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT
    CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC
    CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG
    AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG
    ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA
    CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA
    ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA
    GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC
    CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT
    AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT
    TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC
    CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA
    TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA
    AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT
    CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG
    GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA
    ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC
    ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC
    AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC
    ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG
    GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA
    CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG
    GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG
    ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC
    AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC
    AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG
    CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG
    AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT
    GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA
    CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT
    GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC
    ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160822.1
    CDS297..5036
    Translation

    Target ORF information:

    RefSeq Version XM_005160765.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160765.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGCAACA CTCCACCTTC ACAGCTTCAG TCTCCTTCAG GACTGGATCC AGCCCAGACC 
    CCTGGTGTTA GGAGCGATTG TGGGATCAGA CGCAGGCTTT TGGCCTCGAA GGTCCACCAG
    AGACCCGAAT CACTGTGGAC ACCTAAACCA ATGCTAAGAG CAGAAGTTAA AGGCTCACAA
    GGAGACACAC TGACGCGCTC TCAGTCCTGT TGCAAGAGGA ACTCATTGTC TTGCTTTCCC
    ACTGTATCTA ATCTGGATGA GGAGAAAAAG TGGACCGTGC ACTACACTGC CCCATGGCAC
    CAGCAGGAGA ACGTCTTTCT GCCAGGCTCC AGACCGGCCT GTGTGGAAGA TCTTCACCGT
    CAGGCCAAAG TGAACCTGAA AACAGCTCTC AGAGAATGTG ATAAACTGAG GAAGGATGGC
    TTCCGTAGCT CGCAGTACTA CTCACAGGGG CCCACTTTCT CTGGCTCCTC AAGCTCCCAA
    CATGAGGACG AGGACCTTGA AGGCGATGAC GCTGACAAAA AGTCCACAGC ATCTTCAGGA
    GAGGAAGAGA AAGACTCCTG CCAAATGAGA GCGCAAAGTC CGGCACAGGT GGAAGGAGTC
    GAGGTTGATG GACAAATGTC GAGCTCCAAA GCCTCAGTCC TGCCCACTCC AGAGGAGAAG
    ATGAGGCAAC AGGCACAGGC CGTTCTCACA GATATTGTCC CCATTAATGT CACGGGAGAG
    ACGTTTGACA GGCAGGCCAG TATCCGCCGC TCCCTAATAA ACACTGACAC ACTGGTCCGC
    CGACCCAAGA AGGTTAAACG GAGAAAGACT ATTTCAGGGG TGCCTGACAG CGTCCAACAG
    GAACTAGCGG CTAAAGGGCG TGGAGGAGAG CTTCGACCAC AGTCCATGTT TATCCCTGGA
    CAGTATTCAA CACTTGGGCG AGTCATGAAT GGGAATTCAA CTCTGCGTCA ATCTGTAACA
    AAAGATTCTG GCTGCCAGAC TGAGGCGGTG AAAATCGTCC CACCCTCAGT AAGGAGAATA
    CGGGCTCAGA AGGGTCAAGG AATTGCTGCT CAGATGGCTG GCATCTCCAC CTCAGCCACA
    AACATCTCTT CTGTTTCTGA TGGGAGCTCC ACTGGAGGTT CGATTGTTGC AATGGCACCA
    CAGTTCGGCA ATGATATCCA ACGCTTTCAC AGCTTGCCAC GGGGTGCACG GGTCTCTCTA
    AATGCAGAGC CCATTTACAG TAGCACCCCT TTCAGAAAGG AAGACTCCTG TGCAAAAGCA
    CCTAAGCAGA TTGGAAAATT ACAAGTAGAC AATACTGCAG TGCACATGAG GAATGCCCCC
    AGGGTGTGTA CTCAAGCCCG CCCAAAATCT CAGGAGGTGC GAGGATCTCA AAGGGAGTGG
    GGTTCTGTCT CGGGTCCAGC CTGTGTAGTT TCTCCACATG CAGCCTACTC AACCTCCCTT
    ATACCTAATG CTACTCTGTC ATGTTCTGCT GAGGTTTTTG CTCTTCACAG CAAATCAAGT
    CCTGGCCAGA GCCCACTTTC TGGGTCACCA TACACTAAAG CTAGACCTCT TAGCATGGTC
    TCAGTTGTCA ACAGTGGGAG TACAAGTAGT GTGGGCACAG GATCTCACAC CCCAGATGCA
    GGCATGAAAG ATACCTGCAG TGAGACTGGC CAATCTGATA GCAGTTTGCA CAGCCACAGT
    ACCATTGCCG CAGGAACATT GTCTTCAGAT GAGCAGTGGA TTTACGATAC TCCTGATAAT
    GCCTTGCCCA GAAGGACAAT GACTTCCAGC TGCTCAACAC CCATAAATCA TCTATATAGT
    AGCCTTGAAC GCTCCTCAAA AGGCACAGAC TCTAGTTCAC TGTACTCTAT GGACAATGAT
    GGCTACTACA CTTCCATGCA TCTGGATTCA GGCCTTAGGT CAAGAAGCCA TGGTAGCGGC
    CATGGTCATG GCATAGGAGG AAGAGCAGTA AGACACAGTA TGTACGAGTG CTTGGGTGAG
    CAAGAGGATC GAAATAGCTT GTGCAGCGAC CACTCGCTAT CTCGTTCCAT TTCTCTACGC
    AAGCCTAAGA AACCACCCCT TCCACCTGCA CGCACAGACT CTCTACGGAG AAAGCCTGGG
    AAAAACAGCT CTCCCTCTGC CAATATTGGG CAGTCAAATA TTAGGAATGG TTCTCTTCTC
    AATGAATCAT TGATTGCCAC TCTCCAACAG TCACTTCAGA ATGGGCTTAA AGGCAAAGGA
    TCCTCCACGT CACCATCTCA TAGCCCTTGT AGTGACTATG AGGACCCCTG GATGCTTCGT
    CCAAGGAGCC AGAGCAGCAT CAGTGCAAGT AGTAGTGGTG TATCAGCTAC AGGAATGGCT
    CATGTATACT CCATCTGTCC CGTCACACCA TCTCATAGTG ACACAAGTAG CCTCCGTTCT
    GATTACGCCG ACTCCTGGGG CTACTACATG GAATACCCTC GTCCATCTGG AGATCAGACA
    CAGTCACCAT GTACCAATTC ACACTCTGCT GGACAAGTGT CTGAGATGGC AAATGGAAGA
    GGCCCTCTCA ATGGTAACCA GGCTAATCTT CCTGCTGTTC AGGAAGGAAG TGACATGTCT
    GCTAAGCCCA GGACAGGCAC CTCTTCACCG GACAGGGTAC ATCGGTTGAC TTCTCCATCA
    AGTGGATACT CAAGTCAGTC AAACACTCCA ACTGCTGGTA CTCCCGTTCC CTCCTTCATG
    AGATGCATGT CCCCATCAGG CAAGCCCAAA CCAAGAGTGC CTGAAAGAAA GTCATCCTTG
    CTTTCATCTG TATCTATATC CTCCTCTTCC ACCTCTCTTT CATCCAACAC CTCTGATTCT
    AATAGGAGCA ATATTCCACC TCCTCCTCCT CTTCCAGCTG CCCCTGTGGT ACTTGTACCT
    CTTAATCCTC CATCCTTTCC TCCTCCCCTT CCACCCCCAA GCCCTGTGTG CACTGAAGAC
    CAGAAATTTC CACCTCCGCC ACCTCCTTTA CCCACAACTC CCCATCAGCA AGCATCCATG
    AACCCTCTTC AAAACAATTC CTCCCCTGAA TTTCCACCCC CTCCACCTCC AGAAGTGTTG
    ACCGACCCTG CCTGTTCAGG CTTTAATAGT TCCTTCAGCC CTCCACCACC TCCCCCTCCA
    CCACCACTGC CTACTTATTC TCCATCCCAA AATCAACATT TACCCCACAT GACGCCACAA
    ACACCTTCTT CTGCCTCCTT GAAAGAAATT AAGGGCTGTC TTAAACCAGT GAACTTAGAA
    GGAAGACCAG TGTTGCCTCA ATCTGAGGAA CCTAATAAGG TTAGCATGCC TTTGATTACC
    CCATTTGCAC TGCAGAGTGT GCAGCTTCGG CCAGCAAAAC GTCCAGAAAA CAATGAAGTT
    AGCAGGACAG ACAGACCCCA AACACCAGAA AAAACTCACC CCACAGTTCC TCAGATTTCT
    TGTATATCTC CAAATGTAAG CCTTTCATCT ACAGAGGAAA AACCCTCAAT CCAGCATAGC
    CAGGATGGCC TTGTTGAAGC ACAAACTGAC TATAGTGTAA AATCCAATTC TGATCCAACA
    TCCTTCCTTA CAAATGGGCC CATAGATCTT GGTGAGGCCA CAGATGAAGT AGATGGTTTA
    AAGACTTTCC TCTCAACACA AGTGGAAAAG TCACTAAACT CTACACCCAA GAAAAAGCCT
    CCAATGATTT CCAAAAAGCC CAAGTTTTCT CTTACTTTCT CCTCTGTGGA TCATGTAAGG
    GATTTTGTGA GTGCTGAAGT TCTGGACCCC ATACCTGTGC TGGAAAAAGA GGAAGAGACA
    ACTGAGAAAA GTAGCTTTGA CATTTCCGTA CCTCCTGTAG AGATAAGAGA CATTTCTCCC
    ACAAATGGTG AATCTCAGGA GTTCTCACAA TCCTCTGGTA CCACTATTCT TGATGTAGAC
    AAGAGCACCT CTGAAGCAGA GGATGAGGAA GAAAGAGATG ACGAAGATGT AACCAGCAGC
    ACAGGATCGT TCAGTTCTAA AGATGATGAA AATGGTGATG TGTTTGAATC CAGCACGTTG
    GACGTGGCTC AATCACCCAG CATCAACGGT GACAGCTGTG GGGACATGGT GACCCCCACA
    CGGCCCCGCA CCACAGAGGA CCTGTTTGCA GCCATTCACA GGTCGAAGAG AAAAGTCCTG
    GGGCGCAAGG AATCAGAAGA GGATCGCTCC CGGGGTCCTC TATCTCCACC GGTCACCCCG
    ACAGCACAGA GCCCCAGCTT GACCTCCTCT CTGCCCCGGC AATCAGGCTC CATCCAGCGC
    AGCCTCCGCA AATCTGCAAC CAGCAGTGAC ACCTTCAAGG CCCTCCTGCT AAAGAAAGGC
    AGTCGCTCTG AAAGCAGTTT CCGCATGTCT GCAACCGAGA TGCTGCGCAG CACCGACCCG
    CGCTTTCAAC GCACCCGCTC CCTCGACTCC TCCTTTGATC CGGCGTCTCC AACATCCGAG
    AGCCCCTGCT CTTCTCCAGG CCGAAGCAAG AGGGTGGCAG ATGACTGGGC GCGCAATGAT
    GGAATGTTTT CAACTTCGCC ATCTTTGATA GGCCCAAAAT ACGGACGATC CCGAACGCCA
    CCATCTGCTG CTAGCAGCAA ATACAACGCC CGCTGCCGCA TCCTCAGCAG CCCCATGACT
    GTCATCTGCG AGAGGGATGG AGAACTAACC GACTGTGAAG ACCCCGTTTC TCCGTCAAAC
    ACACCCCTTC CCATCTCTCA GGACTCAAAC AGCACTTTAT GTGAGCAGAG CAGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25048
    Clone ID Related Accession (Same CDS sequence) XM_005160766.4 , XM_005160766.3
    Accession Version XM_005160766.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160766.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
    ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG
    TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA
    GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG
    AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA
    GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA
    GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA
    CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC
    GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA
    ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC
    TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT
    CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC
    CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA
    AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA
    GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC
    ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG
    GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT
    GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG
    AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC
    TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC
    ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA
    GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA
    AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT
    GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT
    TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG
    TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA
    AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG
    ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT
    TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT
    TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC
    ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG
    GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA
    GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC
    AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA
    CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT
    ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC
    CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC
    CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT
    GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC
    ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC
    TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC
    TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT
    AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT
    TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC
    ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG
    CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC
    TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT
    CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT
    CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT
    CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC
    CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT
    AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA
    TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA
    GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT
    GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG
    CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA
    CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT
    TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA
    ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA
    GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG
    GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG
    TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG
    GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT
    TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC
    TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT
    GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT
    GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC
    AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG
    TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT
    CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC
    TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC
    AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC
    ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC
    GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA
    AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT
    TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC
    AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA
    CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC
    TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160823.1
    CDS1469..6184
    Translation

    Target ORF information:

    RefSeq Version XM_005160766.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160766.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
    ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG
    TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA
    GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG
    AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA
    GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA
    GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA
    CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC
    GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA
    ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC
    TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT
    CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC
    CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA
    AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA
    GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC
    ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG
    GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT
    GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG
    AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC
    TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC
    ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA
    GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA
    AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT
    GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT
    TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG
    TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA
    AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG
    ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT
    TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT
    TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC
    ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG
    GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA
    GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC
    AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA
    CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT
    ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC
    CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC
    CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT
    GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC
    ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC
    TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC
    TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT
    AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT
    TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC
    ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG
    CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC
    TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT
    CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT
    CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT
    CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC
    CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT
    AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA
    TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA
    GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT
    GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG
    CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA
    CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT
    TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA
    ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA
    GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG
    GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG
    TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG
    GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT
    TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC
    TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT
    GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT
    GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC
    AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG
    TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT
    CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC
    TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC
    AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC
    ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC
    GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA
    AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT
    TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC
    AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA
    CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC
    TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25048
    Clone ID Related Accession (Same CDS sequence) XM_005160766.4 , XM_005160766.3
    Accession Version XM_005160766.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
    ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG
    TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA
    GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG
    AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA
    GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA
    GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA
    CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC
    GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA
    ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC
    TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT
    CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC
    CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA
    AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA
    GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC
    ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG
    GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT
    GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG
    AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC
    TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC
    ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA
    GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA
    AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT
    GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT
    TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG
    TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA
    AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG
    ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT
    TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT
    TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC
    ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG
    GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA
    GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC
    AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA
    CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT
    ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC
    CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC
    CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT
    GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC
    ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC
    TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC
    TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT
    AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT
    TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC
    ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG
    CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC
    TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT
    CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT
    CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT
    CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC
    CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT
    AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA
    TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA
    GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT
    GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG
    CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA
    CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT
    TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA
    ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA
    GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG
    GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG
    TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG
    GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT
    TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC
    TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT
    GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT
    GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC
    AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG
    TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT
    CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC
    TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC
    AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC
    ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC
    GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA
    AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT
    TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC
    AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA
    CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC
    TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160823.1
    CDS1487..6202
    Translation

    Target ORF information:

    RefSeq Version XM_005160766.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160766.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGATGAGGG ACAAGCGTTC TGGGTCTTTT AGGAGGGACA AGACAGAGAA GCCTGCGCCG 
    ATCTCTCGGG CTCTCAGCTG GCTGAGCGTG TCCTCTCTGT CACAGCAGAC ACGCAAACTG
    TTCCGCAGCC AGAACAGCCT TCACAACCAC TCACACACTC CAGAGGGAGG AGATGATGAA
    GATGACAACT GGGTTTATGA GCCCCAGCAC TACATAGCTG TATCTAATCT GGATGAGGAG
    AAAAAGTGGA CCGTGCACTA CACTGCCCCA TGGCACCAGC AGGAGAACGT CTTTCTGCCA
    GGCTCCAGAC CGGCCTGTGT GGAAGATCTT CACCGTCAGG CCAAAGTGAA CCTGAAAACA
    GCTCTCAGAG AATGTGATAA ACTGAGGAAG GATGGCTTCC GTAGCTCGCA GTACTACTCA
    CAGGGGCCCA CTTTCTCTGG CTCCTCAAGC TCCCAACATG AGGACGAGGA CCTTGAAGGC
    GATGACGCTG ACAAAAAGTC CACAGCATCT TCAGGAGAGG AAGAGAAAGA CTCCTGCCAA
    ATGAGAGCGC AAAGTCCGGC ACAGGTGGAA GGAGTCGAGG TTGATGGACA AATGTCGAGC
    TCCAAAGCCT CAGTCCTGCC CACTCCAGAG GAGAAGATGA GGCAACAGGC ACAGGCCGTT
    CTCACAGATA TTGTCCCCAT TAATGTCACG GGAGAGACGT TTGACAGGCA GGCCAGTATC
    CGCCGCTCCC TAATAAACAC TGACACACTG GTCCGCCGAC CCAAGAAGGT TAAACGGAGA
    AAGACTATTT CAGGGGTGCC TGACAGCGTC CAACAGGAAC TAGCGGCTAA AGGGCGTGGA
    GGAGAGCTTC GACCACAGTC CATGTTTATC CCTGGACAGT ATTCAACACT TGGGCGAGTC
    ATGAATGGGA ATTCAACTCT GCGTCAATCT GTAACAAAAG ATTCTGGCTG CCAGACTGAG
    GCGGTGAAAA TCGTCCCACC CTCAGTAAGG AGAATACGGG CTCAGAAGGG TCAAGGAATT
    GCTGCTCAGA TGGCTGGCAT CTCCACCTCA GCCACAAACA TCTCTTCTGT TTCTGATGGG
    AGCTCCACTG GAGGTTCGAT TGTTGCAATG GCACCACAGT TCGGCAATGA TATCCAACGC
    TTTCACAGCT TGCCACGGGG TGCACGGGTC TCTCTAAATG CAGAGCCCAT TTACAGTAGC
    ACCCCTTTCA GAAAGGAAGA CTCCTGTGCA AAAGCACCTA AGCAGATTGG AAAATTACAA
    GTAGACAATA CTGCAGTGCA CATGAGGAAT GCCCCCAGGG TGTGTACTCA AGCCCGCCCA
    AAATCTCAGG AGGTGCGAGG ATCTCAAAGG GAGTGGGGTT CTGTCTCGGG TCCAGCCTGT
    GTAGTTTCTC CACATGCAGC CTACTCAACC TCCCTTATAC CTAATGCTAC TCTGTCATGT
    TCTGCTGAGG TTTTTGCTCT TCACAGCAAA TCAAGTCCTG GCCAGAGCCC ACTTTCTGGG
    TCACCATACA CTAAAGCTAG ACCTCTTAGC ATGGTCTCAG TTGTCAACAG TGGGAGTACA
    AGTAGTGTGG GCACAGGATC TCACACCCCA GATGCAGGCA TGAAAGATAC CTGCAGTGAG
    ACTGGCCAAT CTGATAGCAG TTTGCACAGC CACAGTACCA TTGCCGCAGG AACATTGTCT
    TCAGATGAGC AGTGGATTTA CGATACTCCT GATAATGCCT TGCCCAGAAG GACAATGACT
    TCCAGCTGCT CAACACCCAT AAATCATCTA TATAGTAGCC TTGAACGCTC CTCAAAAGGC
    ACAGACTCTA GTTCACTGTA CTCTATGGAC AATGATGGCT ACTACACTTC CATGCATCTG
    GATTCAGGCC TTAGGTCAAG AAGCCATGGT AGCGGCCATG GTCATGGCAT AGGAGGAAGA
    GCAGTAAGAC ACAGTATGTA CGAGTGCTTG GGTGAGCAAG AGGATCGAAA TAGCTTGTGC
    AGCGACCACT CGCTATCTCG TTCCATTTCT CTACGCAAGC CTAAGAAACC ACCCCTTCCA
    CCTGCACGCA CAGACTCTCT ACGGAGAAAG CCTGGGAAAA ACAGCTCTCC CTCTGCCAAT
    ATTGGGCAGT CAAATATTAG GAATGGTTCT CTTCTCAATG AATCATTGAT TGCCACTCTC
    CAACAGTCAC TTCAGAATGG GCTTAAAGGC AAAGGATCCT CCACGTCACC ATCTCATAGC
    CCTTGTAGTG ACTATGAGGA CCCCTGGATG CTTCGTCCAA GGAGCCAGAG CAGCATCAGT
    GCAAGTAGTA GTGGTGTATC AGCTACAGGA ATGGCTCATG TATACTCCAT CTGTCCCGTC
    ACACCATCTC ATAGTGACAC AAGTAGCCTC CGTTCTGATT ACGCCGACTC CTGGGGCTAC
    TACATGGAAT ACCCTCGTCC ATCTGGAGAT CAGACACAGT CACCATGTAC CAATTCACAC
    TCTGCTGGAC AAGTGTCTGA GATGGCAAAT GGAAGAGGCC CTCTCAATGG TAACCAGGCT
    AATCTTCCTG CTGTTCAGGA AGGAAGTGAC ATGTCTGCTA AGCCCAGGAC AGGCACCTCT
    TCACCGGACA GGGTACATCG GTTGACTTCT CCATCAAGTG GATACTCAAG TCAGTCAAAC
    ACTCCAACTG CTGGTACTCC CGTTCCCTCC TTCATGAGAT GCATGTCCCC ATCAGGCAAG
    CCCAAACCAA GAGTGCCTGA AAGAAAGTCA TCCTTGCTTT CATCTGTATC TATATCCTCC
    TCTTCCACCT CTCTTTCATC CAACACCTCT GATTCTAATA GGAGCAATAT TCCACCTCCT
    CCTCCTCTTC CAGCTGCCCC TGTGGTACTT GTACCTCTTA ATCCTCCATC CTTTCCTCCT
    CCCCTTCCAC CCCCAAGCCC TGTGTGCACT GAAGACCAGA AATTTCCACC TCCGCCACCT
    CCTTTACCCA CAACTCCCCA TCAGCAAGCA TCCATGAACC CTCTTCAAAA CAATTCCTCC
    CCTGAATTTC CACCCCCTCC ACCTCCAGAA GTGTTGACCG ACCCTGCCTG TTCAGGCTTT
    AATAGTTCCT TCAGCCCTCC ACCACCTCCC CCTCCACCAC CACTGCCTAC TTATTCTCCA
    TCCCAAAATC AACATTTACC CCACATGACG CCACAAACAC CTTCTTCTGC CTCCTTGAAA
    GAAATTAAGG GCTGTCTTAA ACCAGTGAAC TTAGAAGGAA GACCAGTGTT GCCTCAATCT
    GAGGAACCTA ATAAGGTTAG CATGCCTTTG ATTACCCCAT TTGCACTGCA GAGTGTGCAG
    CTTCGGCCAG CAAAACGTCC AGAAAACAAT GAAGTTAGCA GGACAGACAG ACCCCAAACA
    CCAGAAAAAA CTCACCCCAC AGTTCCTCAG ATTTCTTGTA TATCTCCAAA TGTAAGCCTT
    TCATCTACAG AGGAAAAACC CTCAATCCAG CATAGCCAGG ATGGCCTTGT TGAAGCACAA
    ACTGACTATA GTGTAAAATC CAATTCTGAT CCAACATCCT TCCTTACAAA TGGGCCCATA
    GATCTTGGTG AGGCCACAGA TGAAGTAGAT GGTTTAAAGA CTTTCCTCTC AACACAAGTG
    GAAAAGTCAC TAAACTCTAC ACCCAAGAAA AAGCCTCCAA TGATTTCCAA AAAGCCCAAG
    TTTTCTCTTA CTTTCTCCTC TGTGGATCAT GTAAGGGATT TTGTGAGTGC TGAAGTTCTG
    GACCCCATAC CTGTGCTGGA AAAAGAGGAA GAGACAACTG AGAAAAGTAG CTTTGACATT
    TCCGTACCTC CTGTAGAGAT AAGAGACATT TCTCCCACAA ATGGTGAATC TCAGGAGTTC
    TCACAATCCT CTGGTACCAC TATTCTTGAT GTAGACAAGA GCACCTCTGA AGCAGAGGAT
    GAGGAAGAAA GAGATGACGA AGATGTAACC AGCAGCACAG GATCGTTCAG TTCTAAAGAT
    GATGAAAATG GTGATGTGTT TGAATCCAGC ACGTTGGACG TGGCTCAATC ACCCAGCATC
    AACGGTGACA GCTGTGGGGA CATGGTGACC CCCACACGGC CCCGCACCAC AGAGGACCTG
    TTTGCAGCCA TTCACAGGTC GAAGAGAAAA GTCCTGGGGC GCAAGGAATC AGAAGAGGAT
    CGCTCCCGGG GTCCTCTATC TCCACCGGTC ACCCCGACAG CACAGAGCCC CAGCTTGACC
    TCCTCTCTGC CCCGGCAATC AGGCTCCATC CAGCGCAGCC TCCGCAAATC TGCAACCAGC
    AGTGACACCT TCAAGGCCCT CCTGCTAAAG AAAGGCAGTC GCTCTGAAAG CAGTTTCCGC
    ATGTCTGCAA CCGAGATGCT GCGCAGCACC GACCCGCGCT TTCAACGCAC CCGCTCCCTC
    GACTCCTCCT TTGATCCGGC GTCTCCAACA TCCGAGAGCC CCTGCTCTTC TCCAGGCCGA
    AGCAAGAGGG TGGCAGATGA CTGGGCGCGC AATGATGGAA TGTTTTCAAC TTCGCCATCT
    TTGATAGGCC CAAAATACGG ACGATCCCGA ACGCCACCAT CTGCTGCTAG CAGCAAATAC
    AACGCCCGCT GCCGCATCCT CAGCAGCCCC ATGACTGTCA TCTGCGAGAG GGATGGAGAA
    CTAACCGACT GTGAAGACCC CGTTTCTCCG TCAAACACAC CCCTTCCCAT CTCTCAGGAC
    TCAAACAGCA CTTTATGTGA GCAGAGCAGC AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25049
    Clone ID Related Accession (Same CDS sequence) XM_005160767.4 , XM_005160767.3
    Accession Version XM_005160767.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160767.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT
    ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA
    TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA
    AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC
    TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA
    ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG
    GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT
    GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG
    AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA
    AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA
    ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC
    AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT
    AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC
    CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC
    AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT
    CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT
    CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG
    GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT
    GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC
    TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT
    TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA
    AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT
    TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA
    GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG
    ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA
    GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC
    CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA
    GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA
    AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT
    GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT
    TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC
    TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG
    TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC
    TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA
    CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC
    ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA
    GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA
    GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT
    CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG
    ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG
    AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT
    TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC
    AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT
    CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC
    CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT
    GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA
    GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG
    AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT
    CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG
    GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC
    ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT
    GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA
    ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC
    AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG
    ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA
    AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG
    GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC
    ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA
    AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC
    ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA
    ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG
    CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC
    CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC
    CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT
    CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160824.1
    CDS496..5184
    Translation

    Target ORF information:

    RefSeq Version XM_005160767.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160767.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT
    ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA
    TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA
    AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC
    TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA
    ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG
    GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT
    GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG
    AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA
    AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA
    ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC
    AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT
    AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC
    CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC
    AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT
    CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT
    CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG
    GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT
    GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC
    TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT
    TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA
    AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT
    TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA
    GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG
    ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA
    GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC
    CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA
    GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA
    AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT
    GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT
    TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC
    TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG
    TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC
    TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA
    CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC
    ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA
    GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA
    GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT
    CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG
    ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG
    AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT
    TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC
    AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT
    CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC
    CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT
    GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA
    GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG
    AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT
    CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG
    GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC
    ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT
    GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA
    ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC
    AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG
    ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA
    AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG
    GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC
    ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA
    AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC
    ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA
    ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG
    CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC
    CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC
    CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT
    CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25049
    Clone ID Related Accession (Same CDS sequence) XM_005160767.4 , XM_005160767.3
    Accession Version XM_005160767.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4689bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160767.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT
    ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA
    TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA
    AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC
    TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA
    ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG
    GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT
    GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG
    AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA
    AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA
    ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC
    AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT
    AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC
    CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC
    AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT
    CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT
    CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG
    GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT
    GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC
    TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT
    TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA
    AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT
    TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA
    GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG
    ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA
    GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC
    CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA
    GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA
    AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT
    GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT
    TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC
    TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG
    TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC
    TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA
    CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC
    ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA
    GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA
    GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT
    CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG
    ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG
    AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT
    TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC
    AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT
    CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC
    CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT
    GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA
    GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG
    AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT
    CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG
    GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC
    ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT
    GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA
    ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC
    AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG
    ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA
    AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG
    GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC
    ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA
    AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC
    ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA
    ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG
    CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC
    CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC
    CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT
    CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005160824.1
    CDS720..5408
    Translation

    Target ORF information:

    RefSeq Version XM_005160767.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005160767.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGCCGTTTC CCGAGAGAGC CGTTGAGCCT CAGCTGCTGT GCCGGTTGAG GGGGAGCGAT 
    GGACCCGAGA AGAGCTTTAT AACACCGGAC GGTCGCAAAG TTCGCAAACC GGTTTTGTTT
    TCCTCTCTGG AGGAAGTTTG TTGTCATACG TTCACCAGCA TCCTACATCA GCTGTCCGAT
    CTATCCCGAC ACGCCAGTGA CATATTCCTG GGAATTGAGA CTCAAGCTGG GCTAATCTCG
    CACAGGACAT CGAGGATTCA AGCGCGTTTG GAGAGGATAC AGCACACCGT TCAAACGCTC
    GATCCCAAAT CGGTTAAAAT ACCTGTATCT AATCTGGATG AGGAGAAAAA GTGGACCGTG
    CACTACACTG CCCCATGGCA CCAGCAGGAG AACGTCTTTC TGCCAGGCTC CAGACCGGCC
    TGTGTGGAAG ATCTTCACCG TCAGGCCAAA GTGAACCTGA AAACAGCTCT CAGAGAATGT
    GATAAACTGA GGAAGGATGG CTTCCGTAGC TCGCAGTACT ACTCACAGGG GCCCACTTTC
    TCTGGCTCCT CAAGCTCCCA ACATGAGGAC GAGGACCTTG AAGGCGATGA CGCTGACAAA
    AAGTCCACAG CATCTTCAGG AGAGGAAGAG AAAGACTCCT GCCAAATGAG AGCGCAAAGT
    CCGGCACAGG TGGAAGGAGT CGAGGTTGAT GGACAAATGT CGAGCTCCAA AGCCTCAGTC
    CTGCCCACTC CAGAGGAGAA GATGAGGCAA CAGGCACAGG CCGTTCTCAC AGATATTGTC
    CCCATTAATG TCACGGCGGC TAAAGGGCGT GGAGGAGAGC TTCGACCACA GTCCATGTTT
    ATCCCTGGAC AGTATTCAAC ACTTGGGCGA GTCATGAATG GGAATTCAAC TCTGCGTCAA
    TCTGTAACAA AAGATTCTGG CTGCCAGACT GAGGCGGTGA AAATCGTCCC ACCCTCAGTA
    AGGAGAATAC GGGCTCAGAA GGGTCAAGGA ATTGCTGCTC AGATGGCTGG CATCTCCACC
    TCAGCCACAA ACATCTCTTC TGTTTCTGAT GGGAGCTCCA CTGGAGGTTC GATTGTTGCA
    ATGGCACCAC AGTTCGGCAA TGATATCCAA CGCTTTCACA GCTTGCCACG GGGTGCACGG
    GTCTCTCTAA ATGCAGAGCC CATTTACAGT AGCACCCCTT TCAGAAAGGA AGACTCCTGT
    GCAAAAGCAC CTAAGCAGAT TGGAAAATTA CAAGTAGACA ATACTGCAGT GCACATGAGG
    AATGCCCCCA GGGTGTGTAC TCAAGCCCGC CCAAAATCTC AGGAGGTGCG AGGATCTCAA
    AGGGAGTGGG GTTCTGTCTC GGGTCCAGCC TGTGTAGTTT CTCCACATGC AGCCTACTCA
    ACCTCCCTTA TACCTAATGC TACTCTGTCA TGTTCTGCTG AGGTTTTTGC TCTTCACAGC
    AAATCAAGTC CTGGCCAGAG CCCACTTTCT GGGTCACCAT ACACTAAAGC TAGACCTCTT
    AGCATGGTCT CAGTTGTCAA CAGTGGGAGT ACAAGTAGTG TGGGCACAGG ATCTCACACC
    CCAGATGCAG GCATGAAAGA TACCTGCAGT GAGACTGGCC AATCTGATAG CAGTTTGCAC
    AGCCACAGTA CCATTGCCGC AGGAACATTG TCTTCAGATG AGCAGTGGAT TTACGATACT
    CCTGATAATG CCTTGCCCAG AAGGACAATG ACTTCCAGCT GCTCAACACC CATAAATCAT
    CTATATAGTA GCCTTGAACG CTCCTCAAAA GGCACAGACT CTAGTTCACT GTACTCTATG
    GACAATGATG GCTACTACAC TTCCATGCAT CTGGATTCAG GCCTTAGGTC AAGAAGCCAT
    GGTAGCGGCC ATGGTCATGG CATAGGAGGA AGAGCAGTAA GACACAGTAT GTACGAGTGC
    TTGGGTGAGC AAGAGGATCG AAATAGCTTG TGCAGCGACC ACTCGCTATC TCGTTCCATT
    TCTCTACGCA AGCCTAAGAA ACCACCCCTT CCACCTGCAC GCACAGACTC TCTACGGAGA
    AAGCCTGGGA AAAACAGCTC TCCCTCTGCC AATATTGGGC AGTCAAATAT TAGGAATGGT
    TCTCTTCTCA ATGAATCATT GATTGCCACT CTCCAACAGT CACTTCAGAA TGGGCTTAAA
    GGCAAAGGAT CCTCCACGTC ACCATCTCAT AGCCCTTGTA GTGACTATGA GGACCCCTGG
    ATGCTTCGTC CAAGGAGCCA GAGCAGCATC AGTGCAAGTA GTAGTGGTGT ATCAGCTACA
    GGAATGGCTC ATGTATACTC CATCTGTCCC GTCACACCAT CTCATAGTGA CACAAGTAGC
    CTCCGTTCTG ATTACGCCGA CTCCTGGGGC TACTACATGG AATACCCTCG TCCATCTGGA
    GATCAGACAC AGTCACCATG TACCAATTCA CACTCTGCTG GACAAGTGTC TGAGATGGCA
    AATGGAAGAG GCCCTCTCAA TGGTAACCAG GCTAATCTTC CTGCTGTTCA GGAAGGAAGT
    GACATGTCTG CTAAGCCCAG GACAGGCACC TCTTCACCGG ACAGGGTACA TCGGTTGACT
    TCTCCATCAA GTGGATACTC AAGTCAGTCA AACACTCCAA CTGCTGGTAC TCCCGTTCCC
    TCCTTCATGA GATGCATGTC CCCATCAGGC AAGCCCAAAC CAAGAGTGCC TGAAAGAAAG
    TCATCCTTGC TTTCATCTGT ATCTATATCC TCCTCTTCCA CCTCTCTTTC ATCCAACACC
    TCTGATTCTA ATAGGAGCAA TATTCCACCT CCTCCTCCTC TTCCAGCTGC CCCTGTGGTA
    CTTGTACCTC TTAATCCTCC ATCCTTTCCT CCTCCCCTTC CACCCCCAAG CCCTGTGTGC
    ACTGAAGACC AGAAATTTCC ACCTCCGCCA CCTCCTTTAC CCACAACTCC CCATCAGCAA
    GCATCCATGA ACCCTCTTCA AAACAATTCC TCCCCTGAAT TTCCACCCCC TCCACCTCCA
    GAAGTGTTGA CCGACCCTGC CTGTTCAGGC TTTAATAGTT CCTTCAGCCC TCCACCACCT
    CCCCCTCCAC CACCACTGCC TACTTATTCT CCATCCCAAA ATCAACATTT ACCCCACATG
    ACGCCACAAA CACCTTCTTC TGCCTCCTTG AAAGAAATTA AGGGCTGTCT TAAACCAGTG
    AACTTAGAAG GAAGACCAGT GTTGCCTCAA TCTGAGGAAC CTAATAAGGT TAGCATGCCT
    TTGATTACCC CATTTGCACT GCAGAGTGTG CAGCTTCGGC CAGCAAAACG TCCAGAAAAC
    AATGAAGTTA GCAGGACAGA CAGACCCCAA ACACCAGAAA AAACTCACCC CACAGTTCCT
    CAGATTTCTT GTATATCTCC AAATGTAAGC CTTTCATCTA CAGAGGAAAA ACCCTCAATC
    CAGCATAGCC AGGATGGCCT TGTTGAAGCA CAAACTGACT ATAGTGTAAA ATCCAATTCT
    GATCCAACAT CCTTCCTTAC AAATGGGCCC ATAGATCTTG GTGAGGCCAC AGATGAAGTA
    GATGGTTTAA AGACTTTCCT CTCAACACAA GTGGAAAAGT CACTAAACTC TACACCCAAG
    AAAAAGCCTC CAATGATTTC CAAAAAGCCC AAGTTTTCTC TTACTTTCTC CTCTGTGGAT
    CATGTAAGGG ATTTTGTGAG TGCTGAAGTT CTGGACCCCA TACCTGTGCT GGAAAAAGAG
    GAAGAGACAA CTGAGAAAAG TAGCTTTGAC ATTTCCGTAC CTCCTGTAGA GATAAGAGAC
    ATTTCTCCCA CAAATGGTGA ATCTCAGGAG TTCTCACAAT CCTCTGGTAC CACTATTCTT
    GATGTAGACA AGAGCACCTC TGAAGCAGAG GATGAGGAAG AAAGAGATGA CGAAGATGTA
    ACCAGCAGCA CAGGATCGTT CAGTTCTAAA GATGATGAAA ATGGTGATGT GTTTGAATCC
    AGCACGTTGG ACGTGGCTCA ATCACCCAGC ATCAACGGTG ACAGCTGTGG GGACATGGTG
    ACCCCCACAC GGCCCCGCAC CACAGAGGAC CTGTTTGCAG CCATTCACAG GTCGAAGAGA
    AAAGTCCTGG GGCGCAAGGA ATCAGAAGAG GATCGCTCCC GGGGTCCTCT ATCTCCACCG
    GTCACCCCGA CAGCACAGAG CCCCAGCTTG ACCTCCTCTC TGCCCCGGCA ATCAGGCTCC
    ATCCAGCGCA GCCTCCGCAA ATCTGCAACC AGCAGTGACA CCTTCAAGGC CCTCCTGCTA
    AAGAAAGGCA GTCGCTCTGA AAGCAGTTTC CGCATGTCTG CAACCGAGAT GCTGCGCAGC
    ACCGACCCGC GCTTTCAACG CACCCGCTCC CTCGACTCCT CCTTTGATCC GGCGTCTCCA
    ACATCCGAGA GCCCCTGCTC TTCTCCAGGC CGAAGCAAGA GGGTGGCAGA TGACTGGGCG
    CGCAATGATG GAATGTTTTC AACTTCGCCA TCTTTGATAG GCCCAAAATA CGGACGATCC
    CGAACGCCAC CATCTGCTGC TAGCAGCAAA TACAACGCCC GCTGCCGCAT CCTCAGCAGC
    CCCATGACTG TCATCTGCGA GAGGGATGGA GAACTAACCG ACTGTGAAGA CCCCGTTTCT
    CCGTCAAACA CACCCCTTCC CATCTCTCAG GACTCAAACA GCACTTTATG TGAGCAGAGC
    AGCAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25051
    Clone ID Related Accession (Same CDS sequence) XM_003200723.4 , XM_003200723.5
    Accession Version XM_003200723.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200723.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003200771.1
    CDS159..4712
    Translation

    Target ORF information:

    RefSeq Version XM_003200723.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003200723.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa25051
    Clone ID Related Accession (Same CDS sequence) XM_003200723.4 , XM_003200723.5
    Accession Version XM_003200723.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4554bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200723.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003200771.1
    CDS168..4721
    Translation

    Target ORF information:

    RefSeq Version XM_003200723.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 1b (nhsl1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003200723.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTGTTCA TCGGGACTTC GCTCAAGACA GTCATTAAAT ACTTCAAACG CAAGGCTGTA 
    TCTAATCTGG ATGAGGAGAA AAAGTGGACC GTGCACTACA CTGCCCCATG GCACCAGCAG
    GAGAACGTCT TTCTGCCAGG CTCCAGACCG GCCTGTGTGG AAGATCTTCA CCGTCAGGCC
    AAAGTGAACC TGAAAACAGC TCTCAGAGAA TGTGATAAAC TGAGGAAGGA TGGCTTCCGT
    AGCTCGCAGT ACTACTCACA GGGGCCCACT TTCTCTGGCT CCTCAAGCTC CCAACATGAG
    GACGAGGACC TTGAAGGCGA TGACGCTGAC AAAAAGTCCA CAGCATCTTC AGGAGAGGAA
    GAGAAAGACT CCTGCCAAAT GAGAGCGCAA AGTCCGGCAC AGGTGGAAGG AGTCGAGGTT
    GATGGACAAA TGTCGAGCTC CAAAGCCTCA GTCCTGCCCA CTCCAGAGGA GAAGATGAGG
    CAACAGGCAC AGGCCGTTCT CACAGATATT GTCCCCATTA ATGTCACGGG AGAGACGTTT
    GACAGGCAGG CCAGTATCCG CCGCTCCCTA ATAAACACTG ACACACTGGT CCGCCGACCC
    AAGAAGGTTA AACGGAGAAA GACTATTTCA GGGGTGCCTG ACAGCGTCCA ACAGGAACTA
    GCGGCTAAAG GGCGTGGAGG AGAGCTTCGA CCACAGTCCA TGTTTATCCC TGGACAGTAT
    TCAACACTTG GGCGAGTCAT GAATGGGAAT TCAACTCTGC GTCAATCTGT AACAAAAGAT
    TCTGGCTGCC AGACTGAGGC GGTGAAAATC GTCCCACCCT CAGTAAGGAG AATACGGGCT
    CAGAAGGGTC AAGGAATTGC TGCTCAGATG GCTGGCATCT CCACCTCAGC CACAAACATC
    TCTTCTGTTT CTGATGGGAG CTCCACTGGA GGTTCGATTG TTGCAATGGC ACCACAGTTC
    GGCAATGATA TCCAACGCTT TCACAGCTTG CCACGGGGTG CACGGGTCTC TCTAAATGCA
    GAGCCCATTT ACAGTAGCAC CCCTTTCAGA AAGGAAGACT CCTGTGCAAA AGCACCTAAG
    CAGATTGGAA AATTACAAGT AGACAATACT GCAGTGCACA TGAGGAATGC CCCCAGGGTG
    TGTACTCAAG CCCGCCCAAA ATCTCAGGAG GTGCGAGGAT CTCAAAGGGA GTGGGGTTCT
    GTCTCGGGTC CAGCCTGTGT AGTTTCTCCA CATGCAGCCT ACTCAACCTC CCTTATACCT
    AATGCTACTC TGTCATGTTC TGCTGAGGTT TTTGCTCTTC ACAGCAAATC AAGTCCTGGC
    CAGAGCCCAC TTTCTGGGTC ACCATACACT AAAGCTAGAC CTCTTAGCAT GGTCTCAGTT
    GTCAACAGTG GGAGTACAAG TAGTGTGGGC ACAGGATCTC ACACCCCAGA TGCAGGCATG
    AAAGATACCT GCAGTGAGAC TGGCCAATCT GATAGCAGTT TGCACAGCCA CAGTACCATT
    GCCGCAGGAA CATTGTCTTC AGATGAGCAG TGGATTTACG ATACTCCTGA TAATGCCTTG
    CCCAGAAGGA CAATGACTTC CAGCTGCTCA ACACCCATAA ATCATCTATA TAGTAGCCTT
    GAACGCTCCT CAAAAGGCAC AGACTCTAGT TCACTGTACT CTATGGACAA TGATGGCTAC
    TACACTTCCA TGCATCTGGA TTCAGGCCTT AGGTCAAGAA GCCATGGTAG CGGCCATGGT
    CATGGCATAG GAGGAAGAGC AGTAAGACAC AGTATGTACG AGTGCTTGGG TGAGCAAGAG
    GATCGAAATA GCTTGTGCAG CGACCACTCG CTATCTCGTT CCATTTCTCT ACGCAAGCCT
    AAGAAACCAC CCCTTCCACC TGCACGCACA GACTCTCTAC GGAGAAAGCC TGGGAAAAAC
    AGCTCTCCCT CTGCCAATAT TGGGCAGTCA AATATTAGGA ATGGTTCTCT TCTCAATGAA
    TCATTGATTG CCACTCTCCA ACAGTCACTT CAGAATGGGC TTAAAGGCAA AGGATCCTCC
    ACGTCACCAT CTCATAGCCC TTGTAGTGAC TATGAGGACC CCTGGATGCT TCGTCCAAGG
    AGCCAGAGCA GCATCAGTGC AAGTAGTAGT GGTGTATCAG CTACAGGAAT GGCTCATGTA
    TACTCCATCT GTCCCGTCAC ACCATCTCAT AGTGACACAA GTAGCCTCCG TTCTGATTAC
    GCCGACTCCT GGGGCTACTA CATGGAATAC CCTCGTCCAT CTGGAGATCA GACACAGTCA
    CCATGTACCA ATTCACACTC TGCTGGACAA GTGTCTGAGA TGGCAAATGG AAGAGGCCCT
    CTCAATGGTA ACCAGGCTAA TCTTCCTGCT GTTCAGGAAG GAAGTGACAT GTCTGCTAAG
    CCCAGGACAG GCACCTCTTC ACCGGACAGG GTACATCGGT TGACTTCTCC ATCAAGTGGA
    TACTCAAGTC AGTCAAACAC TCCAACTGCT GGTACTCCCG TTCCCTCCTT CATGAGATGC
    ATGTCCCCAT CAGGCAAGCC CAAACCAAGA GTGCCTGAAA GAAAGTCATC CTTGCTTTCA
    TCTGTATCTA TATCCTCCTC TTCCACCTCT CTTTCATCCA ACACCTCTGA TTCTAATAGG
    AGCAATATTC CACCTCCTCC TCCTCTTCCA GCTGCCCCTG TGGTACTTGT ACCTCTTAAT
    CCTCCATCCT TTCCTCCTCC CCTTCCACCC CCAAGCCCTG TGTGCACTGA AGACCAGAAA
    TTTCCACCTC CGCCACCTCC TTTACCCACA ACTCCCCATC AGCAAGCATC CATGAACCCT
    CTTCAAAACA ATTCCTCCCC TGAATTTCCA CCCCCTCCAC CTCCAGAAGT GTTGACCGAC
    CCTGCCTGTT CAGGCTTTAA TAGTTCCTTC AGCCCTCCAC CACCTCCCCC TCCACCACCA
    CTGCCTACTT ATTCTCCATC CCAAAATCAA CATTTACCCC ACATGACGCC ACAAACACCT
    TCTTCTGCCT CCTTGAAAGA AATTAAGGGC TGTCTTAAAC CAGTGAACTT AGAAGGAAGA
    CCAGTGTTGC CTCAATCTGA GGAACCTAAT AAGGTTAGCA TGCCTTTGAT TACCCCATTT
    GCACTGCAGA GTGTGCAGCT TCGGCCAGCA AAACGTCCAG AAAACAATGA AGTTAGCAGG
    ACAGACAGAC CCCAAACACC AGAAAAAACT CACCCCACAG TTCCTCAGAT TTCTTGTATA
    TCTCCAAATG TAAGCCTTTC ATCTACAGAG GAAAAACCCT CAATCCAGCA TAGCCAGGAT
    GGCCTTGTTG AAGCACAAAC TGACTATAGT GTAAAATCCA ATTCTGATCC AACATCCTTC
    CTTACAAATG GGCCCATAGA TCTTGGTGAG GCCACAGATG AAGTAGATGG TTTAAAGACT
    TTCCTCTCAA CACAAGTGGA AAAGTCACTA AACTCTACAC CCAAGAAAAA GCCTCCAATG
    ATTTCCAAAA AGCCCAAGTT TTCTCTTACT TTCTCCTCTG TGGATCATGT AAGGGATTTT
    GTGAGTGCTG AAGTTCTGGA CCCCATACCT GTGCTGGAAA AAGAGGAAGA GACAACTGAG
    AAAAGTAGCT TTGACATTTC CGTACCTCCT GTAGAGATAA GAGACATTTC TCCCACAAAT
    GGTGAATCTC AGGAGTTCTC ACAATCCTCT GGTACCACTA TTCTTGATGT AGACAAGAGC
    ACCTCTGAAG CAGAGGATGA GGAAGAAAGA GATGACGAAG ATGTAACCAG CAGCACAGGA
    TCGTTCAGTT CTAAAGATGA TGAAAATGGT GATGTGTTTG AATCCAGCAC GTTGGACGTG
    GCTCAATCAC CCAGCATCAA CGGTGACAGC TGTGGGGACA TGGTGACCCC CACACGGCCC
    CGCACCACAG AGGACCTGTT TGCAGCCATT CACAGGTCGA AGAGAAAAGT CCTGGGGCGC
    AAGGAATCAG AAGAGGATCG CTCCCGGGGT CCTCTATCTC CACCGGTCAC CCCGACAGCA
    CAGAGCCCCA GCTTGACCTC CTCTCTGCCC CGGCAATCAG GCTCCATCCA GCGCAGCCTC
    CGCAAATCTG CAACCAGCAG TGACACCTTC AAGGCCCTCC TGCTAAAGAA AGGCAGTCGC
    TCTGAAAGCA GTTTCCGCAT GTCTGCAACC GAGATGCTGC GCAGCACCGA CCCGCGCTTT
    CAACGCACCC GCTCCCTCGA CTCCTCCTTT GATCCGGCGT CTCCAACATC CGAGAGCCCC
    TGCTCTTCTC CAGGCCGAAG CAAGAGGGTG GCAGATGACT GGGCGCGCAA TGATGGAATG
    TTTTCAACTT CGCCATCTTT GATAGGCCCA AAATACGGAC GATCCCGAAC GCCACCATCT
    GCTGCTAGCA GCAAATACAA CGCCCGCTGC CGCATCCTCA GCAGCCCCAT GACTGTCATC
    TGCGAGAGGG ATGGAGAACT AACCGACTGT GAAGACCCCG TTTCTCCGTC AAACACACCC
    CTTCCCATCT CTCAGGACTC AAACAGCACT TTATGTGAGC AGAGCAGCAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.