boka cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	boka
Entrez Gene ID	445218
Full Name	BOK, BCL2 family apoptosis regulator a
Synonyms	bok,zgc:100979
General protein information	Preferred Names BOK, BCL2 family apoptosis regulator a Names bcl-2-related ovarian killer protein homolog A BCL2-related ovarian killer a zBok1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003612.2	NP_001003612.2	bcl-2-related ovarian killer protein homolog A
XM_005165921.3	XP_005165978.1	bcl-2-related ovarian killer protein homolog A isoform X1
XM_005165921.4	XP_005165978.1	bcl-2-related ovarian killer protein homolog A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BOK	NP_115904.1
Xenopus tropicalis (tropical clawed frog)	bok	XP_002936655.1
Canis lupus familiaris (dog)	BOK	XP_003639944.1
Danio rerio (zebrafish)	boka	NP_001003612.1
Pan troglodytes (chimpanzee)	BOK	XP_003309630.2
	BOK	XP_001093115.1
Bos taurus (cattle)	BOK	NP_001092338.1
Rattus norvegicus (Norway rat)	Bok	NP_059008.1
Gallus gallus (chicken)	BOK	NP_990037.1
Drosophila melanogaster (fruit fly)	Buffy	NP_523702.1
Mus musculus (house mouse)	Bok	NP_058058.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish.
Hu P, Liu M, Liu Y, Wang J, Zhang D, Niu H, Jiang S, Wang J, Zhang D, Han B, Xu Q, Chen L
Scientific reports628952(2016 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

S1P-Yap1 signaling regulates endoderm formation required for cardiac precursor cell migration in zebrafish.
Fukui H, Terai K, Nakajima H, Chiba A, Fukuhara S, Mochizuki N
Developmental cell31(1)128-36(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following boka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the boka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11849	XM_005165921.4 Latest version!	Danio rerio BCL2-related ovarian killer a (boka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa98454	NM_001003612.2 Latest version!	Danio rerio BCL2 family apoptosis regulator BOK a (boka), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
ODa11849	XM_005165921.3	Danio rerio BCL2-related ovarian killer a (boka), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

boka ( XM_005165921.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165978.1 Danio rerio BCL2-related ovarian killer a (boka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11849
Clone ID Related Accession (Same CDS sequence)	XM_005165921.3 , XM_005165921.4
Accession Version	XM_005165921.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	bcl-2-related ovarian killer protein homolog A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGCA TGGAGATGTT GCGCCGCTCC TCAGTGTTTG CGGCTGAAGT CATGGAGGTG 
TTTGATCGCT CTCCCACGGA CAAGGAGCTC GTGTCTCAGT CCAAAGTGTT GTGTAGGGAT 
TATATTCACT CCAGACTGCA CCGGGCTGGA ATCGGGTGGT CGAAACCAGA ACACGGGTCT 
GGAGGAACCC TGGCTGAGGT GTCTTCAGTC CTGCTGTGGT TGGGTGATGA GCTAGAGTAC 
CTGCGTCCCA ACGTCTACCG CAACGTAGCT CGACAGCTCA ACATCACAAT AGCCTCTGAG 
AATATCGTCT CTGATGCCTT TCTGGCCGTC GCTGCTGAAA TCTTCTCCAC AGGTGTAACA 
TGGGGGAAGA TTGTGTCTCT GTATGCAGTG GCTGGAGCTC TAGCTGTGGA CTGTGTTCGG 
CATGGACATC CAGCAATGGT GCACACTATC GTCGACTGCA TGGGCGAGTT TGTTCGCAAA 
AGCCTTGCGT CATGGTTAAA GAAGAGAGGA GGCTGGGCGG ACATAACAAA GTGTGTCGTC 
AGTACAGACC CCAGTTTCCA CTCTCACTGG CTAGTAACGG CCGCCTGCGC CTGCGGTCAC 
TACCTTAAGG CTGTGGTTTT CTACCTGCTG AGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165978.1
CDS	412..1053
Translation	MKGMEMLRRSSVFAAEVMEVFDRSPTDKELVSQSKVLCRDYIHSRLHRAGIGWSKPEHGSGGTLAEVSSVLLWLGDELEYLRPNVYRNVARQLNITIASENIVSDAFLAVAAEIFSTGVTWGKIVSLYAVAGALAVDCVRHGHPAMVHTIVDCMGEFVRKSLASWLKKRGGWADITKCVVSTDPSFHSHWLVTAACACGHYLKAVVFYLLREK

Target ORF information:

RefSeq Version	XM_005165921.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio BCL2-related ovarian killer a (boka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165921.3

ORF Insert Sequence:

ATGAAGGGCA TGGAGATGTT GCGCCGCTCC TCAGTGTTTG CGGCTGAAGT CATGGAGGTG 
TTTGATCGCT CTCCCACGGA CAAGGAGCTC GTGTCTCAGT CCAAAGTGTT GTGTAGGGAT 
TATATTCACT CCAGACTGCA CCGGGCTGGA ATCGGGTGGT CGAAACCAGA ACACGGGTCT 
GGAGGAACCC TGGCTGAGGT GTCTTCAGTC CTGCTGTGGT TGGGTGATGA GCTAGAGTAC 
CTGCGTCCCA ACGTCTACCG CAACGTAGCT CGACAGCTCA ACATCACAAT AGCCTCTGAG 
AATATCGTCT CTGATGCCTT TCTGGCCGTC GCTGCTGAAA TCTTCTCCAC AGGTGTAACA 
TGGGGGAAGA TTGTGTCTCT GTATGCAGTG GCTGGAGCTC TAGCTGTGGA CTGTGTTCGG 
CATGGACATC CAGCAATGGT GCACACTATC GTCGACTGCA TGGGCGAGTT TGTTCGCAAA 
AGCCTTGCGT CATGGTTAAA GAAGAGAGGA GGCTGGGCGG ACATAACAAA GTGTGTCGTC 
AGTACAGACC CCAGTTTCCA CTCTCACTGG CTAGTAACGG CCGCCTGCGC CTGCGGTCAC 
TACCTTAAGG CTGTGGTTTT CTACCTGCTG AGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

boka ( XM_005165921.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165978.1 Danio rerio BCL2-related ovarian killer a (boka), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11849
Clone ID Related Accession (Same CDS sequence)	XM_005165921.3 , XM_005165921.4
Accession Version	XM_005165921.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	bcl-2-related ovarian killer protein homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165921.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGGCA TGGAGATGTT GCGCCGCTCC TCAGTGTTTG CGGCTGAAGT CATGGAGGTG 
TTTGATCGCT CTCCCACGGA CAAGGAGCTC GTGTCTCAGT CCAAAGTGTT GTGTAGGGAT 
TATATTCACT CCAGACTGCA CCGGGCTGGA ATCGGGTGGT CGAAACCAGA ACACGGGTCT 
GGAGGAACCC TGGCTGAGGT GTCTTCAGTC CTGCTGTGGT TGGGTGATGA GCTAGAGTAC 
CTGCGTCCCA ACGTCTACCG CAACGTAGCT CGACAGCTCA ACATCACAAT AGCCTCTGAG 
AATATCGTCT CTGATGCCTT TCTGGCCGTC GCTGCTGAAA TCTTCTCCAC AGGTGTAACA 
TGGGGGAAGA TTGTGTCTCT GTATGCAGTG GCTGGAGCTC TAGCTGTGGA CTGTGTTCGG 
CATGGACATC CAGCAATGGT GCACACTATC GTCGACTGCA TGGGCGAGTT TGTTCGCAAA 
AGCCTTGCGT CATGGTTAAA GAAGAGAGGA GGCTGGGCGG ACATAACAAA GTGTGTCGTC 
AGTACAGACC CCAGTTTCCA CTCTCACTGG CTAGTAACGG CCGCCTGCGC CTGCGGTCAC 
TACCTTAAGG CTGTGGTTTT CTACCTGCTG AGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165978.1
CDS	416..1057
Translation	MKGMEMLRRSSVFAAEVMEVFDRSPTDKELVSQSKVLCRDYIHSRLHRAGIGWSKPEHGSGGTLAEVSSVLLWLGDELEYLRPNVYRNVARQLNITIASENIVSDAFLAVAAEIFSTGVTWGKIVSLYAVAGALAVDCVRHGHPAMVHTIVDCMGEFVRKSLASWLKKRGGWADITKCVVSTDPSFHSHWLVTAACACGHYLKAVVFYLLREK

Target ORF information:

RefSeq Version	XM_005165921.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BCL2-related ovarian killer a (boka), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165921.4

ORF Insert Sequence:

ATGAAGGGCA TGGAGATGTT GCGCCGCTCC TCAGTGTTTG CGGCTGAAGT CATGGAGGTG 
TTTGATCGCT CTCCCACGGA CAAGGAGCTC GTGTCTCAGT CCAAAGTGTT GTGTAGGGAT 
TATATTCACT CCAGACTGCA CCGGGCTGGA ATCGGGTGGT CGAAACCAGA ACACGGGTCT 
GGAGGAACCC TGGCTGAGGT GTCTTCAGTC CTGCTGTGGT TGGGTGATGA GCTAGAGTAC 
CTGCGTCCCA ACGTCTACCG CAACGTAGCT CGACAGCTCA ACATCACAAT AGCCTCTGAG 
AATATCGTCT CTGATGCCTT TCTGGCCGTC GCTGCTGAAA TCTTCTCCAC AGGTGTAACA 
TGGGGGAAGA TTGTGTCTCT GTATGCAGTG GCTGGAGCTC TAGCTGTGGA CTGTGTTCGG 
CATGGACATC CAGCAATGGT GCACACTATC GTCGACTGCA TGGGCGAGTT TGTTCGCAAA 
AGCCTTGCGT CATGGTTAAA GAAGAGAGGA GGCTGGGCGG ACATAACAAA GTGTGTCGTC 
AGTACAGACC CCAGTTTCCA CTCTCACTGG CTAGTAACGG CCGCCTGCGC CTGCGGTCAC 
TACCTTAAGG CTGTGGTTTT CTACCTGCTG AGAGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

boka ( NM_001003612.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003612.2 Danio rerio BCL2 family apoptosis regulator BOK a (boka), mRNA.

Price & Availability↑

CloneID	ODa98454
Clone ID Related Accession (Same CDS sequence)	NM_001003612.2
Accession Version	NM_001003612.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	bcl-2-related ovarian killer protein homolog A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU467043.11, EH996835.1 and DT073415.1. On Nov 5, 2015 this sequence version replaced NM_001003612.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01002485.1, BC078217.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGGCA TGGAGATGTT GCGCCGCTCC TCAGTGTTTG CGGCTGAAGT CATGGAGGTG 
TTTGATCGCT CTCCCACGGA CAAGGAGCTC GTGTCTCAGT CCAAAGTGTT GTGTAGGGAT 
TATATTCACT CCAGACTGCA CCGGGCTGGA ATCGGGTGGT CGAAACCAGA ACACGGGTCT 
GGAGGAACCC TGGCTGAGGT GTCTTCAGTC CTGCTGTGGT TGGGTGATGA GCTAGAGTAC 
CTGCGTCCCA ACGTCTACCG CAACGTAGCT CGACAGCTCA ACATCACAAT AGCCTCTGAG 
AATATCGTCT CTGATGCCTT TCTGGCCGTC GCTGCTGAAA TCTTCTCCAC AGAATATAGT 
CGAAAAGGTT TGGAAAAACA TAAGGGTGTA ACATGGGGGA AGATTGTGTC TCTGTATGCA 
GTGGCTGGAG CTCTAGCTGT GGACTGTGTT CGGCATGGAC ATCCAGCAAT GGTGCACACT 
ATCGTCGACT GCATGGGCGA GTTTGTTCGC AAAAGCCTTG CGTCATGGTT AAAGAAGAGA 
GGAGGCTGGG CGGACATAAC AAAGTGTGTC GTCAGTACAG ACCCCAGTTT CCACTCTCAC 
TGGCTAGTAA CGGCCGCCTG CGCCTGCGGT CACTACCTTA AGGCTGTGGT TTTCTACCTG 
CTGAGAGAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003612.2
CDS	409..1083
Translation	MKGMEMLRRSSVFAAEVMEVFDRSPTDKELVSQSKVLCRDYIHSRLHRAGIGWSKPEHGSGGTLAEVSSVLLWLGDELEYLRPNVYRNVARQLNITIASENIVSDAFLAVAAEIFSTEYSRKGLEKHKGVTWGKIVSLYAVAGALAVDCVRHGHPAMVHTIVDCMGEFVRKSLASWLKKRGGWADITKCVVSTDPSFHSHWLVTAACACGHYLKAVVFYLLREK

Target ORF information:

RefSeq Version	NM_001003612.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BCL2 family apoptosis regulator BOK a (boka), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003612.2

ORF Insert Sequence:

ATGAAGGGCA TGGAGATGTT GCGCCGCTCC TCAGTGTTTG CGGCTGAAGT CATGGAGGTG 
TTTGATCGCT CTCCCACGGA CAAGGAGCTC GTGTCTCAGT CCAAAGTGTT GTGTAGGGAT 
TATATTCACT CCAGACTGCA CCGGGCTGGA ATCGGGTGGT CGAAACCAGA ACACGGGTCT 
GGAGGAACCC TGGCTGAGGT GTCTTCAGTC CTGCTGTGGT TGGGTGATGA GCTAGAGTAC 
CTGCGTCCCA ACGTCTACCG CAACGTAGCT CGACAGCTCA ACATCACAAT AGCCTCTGAG 
AATATCGTCT CTGATGCCTT TCTGGCCGTC GCTGCTGAAA TCTTCTCCAC AGAATATAGT 
CGAAAAGGTT TGGAAAAACA TAAGGGTGTA ACATGGGGGA AGATTGTGTC TCTGTATGCA 
GTGGCTGGAG CTCTAGCTGT GGACTGTGTT CGGCATGGAC ATCCAGCAAT GGTGCACACT 
ATCGTCGACT GCATGGGCGA GTTTGTTCGC AAAAGCCTTG CGTCATGGTT AAAGAAGAGA 
GGAGGCTGGG CGGACATAAC AAAGTGTGTC GTCAGTACAG ACCCCAGTTT CCACTCTCAC 
TGGCTAGTAA CGGCCGCCTG CGCCTGCGGT CACTACCTTA AGGCTGTGGT TTTCTACCTG 
CTGAGAGAGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish.
Scientific reports628952(2016 Jun)
Hu P,Liu M,Liu Y,Wang J,Zhang D,Niu H,Jiang S,Wang J,Zhang D,Han B,Xu Q,Chen L

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

S1P-Yap1 signaling regulates endoderm formation required for cardiac precursor cell migration in zebrafish.
Developmental cell31(1)128-36(2014 Oct)
Fukui H,Terai K,Nakajima H,Chiba A,Fukuhara S,Mochizuki N

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boka cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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