lpar2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lpar2a
Entrez Gene ID	445184
Full Name	lysophosphatidic acid receptor 2a
Synonyms	zgc:101053
General protein information	Preferred Names lysophosphatidic acid receptor 2a Names lysophosphatidic acid receptor 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299773.2	XP_009298048.1	lysophosphatidic acid receptor 2 isoform X1
NM_001003578.2	NP_001003578.1	lysophosphatidic acid receptor 2
XM_009299773.3	XP_009298048.1	lysophosphatidic acid receptor 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LPAR3	XP_547308.3
Danio rerio (zebrafish)	lpar2a	NP_001003578.1
Homo sapiens (human)	LPAR3	NP_036284.1
Gallus gallus (chicken)	LPAR3	NP_001107182.1
Xenopus tropicalis (tropical clawed frog)	lpar3	XP_002931719.1
Bos taurus (cattle)	LPAR3	NP_001179670.2
Rattus norvegicus (Norway rat)	Lpar3	NP_076459.1
Pan troglodytes (chimpanzee)	LPAR3	XP_001139887.1
	LPAR3	XP_001107923.1
Mus musculus (house mouse)	Lpar3	NP_075359.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish.
Frisca F, Colquhoun D, Goldshmit Y, ?nk? ML, P?bay A, Kaslin J
Scientific reports637678(2016 Nov)

Pharmacological activation of lysophosphatidic acid receptors regulates erythropoiesis.
Lin KH, Ho YH, Chiang JC, Li MW, Lin SH, Chen WM, Chiang CL, Lin YN, Yang YJ, Chen CN, Lu J, Huang CJ, Tigyi G, Yao CL, Lee H
Scientific reports627050(2016 May)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo.
Nakanaga K, Hama K, Kano K, Sato T, Yukiura H, Inoue A, Saigusa D, Tokuyama H, Tomioka Y, Nishina H, Kawahara A, Aoki J
Journal of biochemistry155(4)235-41(2014 Apr)

Autotaxin regulates vascular development via multiple lysophosphatidic acid (LPA) receptors in zebrafish.
Yukiura H, Hama K, Nakanaga K, Tanaka M, Asaoka Y, Okudaira S, Arima N, Inoue A, Hashimoto T, Arai H, Kawahara A, Nishina H, Aoki J
The Journal of biological chemistry286(51)43972-83(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following lpar2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lpar2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01426	NM_001003578.2 Latest version!	Danio rerio lysophosphatidic acid receptor 2a (lpar2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa41138	XM_009299773.3 Latest version!	Danio rerio lysophosphatidic acid receptor 2a (lpar2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa41138	XM_009299773.2	Danio rerio lysophosphatidic acid receptor 2a (lpar2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lpar2a ( NM_001003578.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003578.1 Danio rerio lysophosphatidic acid receptor 2a (lpar2a), mRNA.

Price & Availability↑

CloneID	ODa01426
Clone ID Related Accession (Same CDS sequence)	NM_001003578.2
Accession Version	NM_001003578.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	lysophosphatidic acid receptor 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078264.1. On Aug 30, 2012 this sequence version replaced NM_001003578.1. ##Evidence-Data-START## Transcript exon combination :: BC078264.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTATTTA ACAGCTGTGA GGCTTTTGAC AATCCAGTGT CTTACTTCTA CAACAGGACT 
GGCAAGCCTA TTAGTCCAAA CTGGCGCACT CATGACTTTG TGGTGGTCGG TCTGGGGCTC 
CCCATTTGTG TTTTCATCAT CCTGGCCAAT ATGTTGGTCA TAGCGGCTAT AATTGTCAAC 
CGAAAGTTCC ACTTCCCAAT TTATTACCTT TTAGGAAACA TGGCGGCCGC TGACCTCTTC 
GCTGGCATCT CGTATTCGTA TCTAGTGTTC CACACAGGAC CCTGGACCAT CAATTTGACC 
ATTAAGGAGT GGTTTGTGCG TGGGGCATTG ATTAACATGA GCCTCACCGC CTCAGTGGTC 
AACCTGTTGG CAGTGGCTGT TGAGCGCCAT CAGACCATCT TCACCATGCA GCTCCACACC 
ACGATGACCC GCTGGCGGGT GGTCATGCTG ATAGTCACCA TCTGGGTGGT GGCAGTTGTC 
ATGGGTTTGG TGCCCACCAT GGGCTGGAAT TGTGTGTGTG ACCAGTTAGG TTGCAGCACG 
GTTGCGCCAC TGTACTCGCG CAGCTTCCTA GTGTTTTGGG CCATCCTCAA CCTTATGGCC 
TTCTTCATCA TGGTGGCCGT TTACACGCAC ATCTTCATCT ACGTCCGCCG GCGGAGTCAA 
GTTATGTCCC CTCACACAGA TCAGCCCTCA AATAATCAGA CGGTTATCAG CCTTATGAAG 
ACCATGTCCA TGATACTGGG TGCCTTTGTG ATCTGCTGGA CTCCAGGTCT GGTGATCCTC 
CTTCTGGACG GTCTGAAGTG CATCGAATGT CACGTCCTGA AGTATGAGAA GTACTGCCTG 
GTTCTGGCCG AGTGCAACTC TCTGGTGAAC CCCATCATCT ACTCTTTTCG GGACGAGGAC 
ATGAGGACCA CCTACAAACG CATTCTCTGC TTCCCTTGGA GAAGAATGCG GCCGCACGAG 
GGGCCTTCTG GCATCCGCTT CGAGCCTGTG AAAACAGGGG CTCCCCCTGA GAAAAACAGC 
CAGAACTCCA GCAGCCCAGA GGCCGCTCCT CCACAAAGCC CATTGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003578.1
CDS	316..1386
Translation	MVFNSCEAFDNPVSYFYNRTGKPISPNWRTHDFVVVGLGLPICVFIILANMLVIAAIIVNRKFHFPIYYLLGNMAAADLFAGISYSYLVFHTGPWTINLTIKEWFVRGALINMSLTASVVNLLAVAVERHQTIFTMQLHTTMTRWRVVMLIVTIWVVAVVMGLVPTMGWNCVCDQLGCSTVAPLYSRSFLVFWAILNLMAFFIMVAVYTHIFIYVRRRSQVMSPHTDQPSNNQTVISLMKTMSMILGAFVICWTPGLVILLLDGLKCIECHVLKYEKYCLVLAECNSLVNPIIYSFRDEDMRTTYKRILCFPWRRMRPHEGPSGIRFEPVKTGAPPEKNSQNSSSPEAAPPQSPLS

Target ORF information:

RefSeq Version	NM_001003578.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysophosphatidic acid receptor 2a (lpar2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003578.2

ORF Insert Sequence:

ATGGTATTTA ACAGCTGTGA GGCTTTTGAC AATCCAGTGT CTTACTTCTA CAACAGGACT 
GGCAAGCCTA TTAGTCCAAA CTGGCGCACT CATGACTTTG TGGTGGTCGG TCTGGGGCTC 
CCCATTTGTG TTTTCATCAT CCTGGCCAAT ATGTTGGTCA TAGCGGCTAT AATTGTCAAC 
CGAAAGTTCC ACTTCCCAAT TTATTACCTT TTAGGAAACA TGGCGGCCGC TGACCTCTTC 
GCTGGCATCT CGTATTCGTA TCTAGTGTTC CACACAGGAC CCTGGACCAT CAATTTGACC 
ATTAAGGAGT GGTTTGTGCG TGGGGCATTG ATTAACATGA GCCTCACCGC CTCAGTGGTC 
AACCTGTTGG CAGTGGCTGT TGAGCGCCAT CAGACCATCT TCACCATGCA GCTCCACACC 
ACGATGACCC GCTGGCGGGT GGTCATGCTG ATAGTCACCA TCTGGGTGGT GGCAGTTGTC 
ATGGGTTTGG TGCCCACCAT GGGCTGGAAT TGTGTGTGTG ACCAGTTAGG TTGCAGCACG 
GTTGCGCCAC TGTACTCGCG CAGCTTCCTA GTGTTTTGGG CCATCCTCAA CCTTATGGCC 
TTCTTCATCA TGGTGGCCGT TTACACGCAC ATCTTCATCT ACGTCCGCCG GCGGAGTCAA 
GTTATGTCCC CTCACACAGA TCAGCCCTCA AATAATCAGA CGGTTATCAG CCTTATGAAG 
ACCATGTCCA TGATACTGGG TGCCTTTGTG ATCTGCTGGA CTCCAGGTCT GGTGATCCTC 
CTTCTGGACG GTCTGAAGTG CATCGAATGT CACGTCCTGA AGTATGAGAA GTACTGCCTG 
GTTCTGGCCG AGTGCAACTC TCTGGTGAAC CCCATCATCT ACTCTTTTCG GGACGAGGAC 
ATGAGGACCA CCTACAAACG CATTCTCTGC TTCCCTTGGA GAAGAATGCG GCCGCACGAG 
GGGCCTTCTG GCATCCGCTT CGAGCCTGTG AAAACAGGGG CTCCCCCTGA GAAAAACAGC 
CAGAACTCCA GCAGCCCAGA GGCCGCTCCT CCACAAAGCC CATTGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lpar2a ( XM_009299773.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298048.1 Danio rerio lysophosphatidic acid receptor 2a (lpar2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41138
Clone ID Related Accession (Same CDS sequence)	XM_009299773.2 , XM_009299773.3
Accession Version	XM_009299773.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysophosphatidic acid receptor 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTTA ACAGCTGTGA GGCTTTTGAC AATCCAGTGT CTTACTTCTA CAACAGGACT 
GGCAAGCCTA TTAGTCCAAA CTGGCGCACT CATGACTTTG TGGTGGTCGG TCTGGGGCTC 
CCCATTTGTG TTTTCATCAT CCTGGCCAAT ATGTTGGTCA TAGCGGCTAT AATTGTCAAC 
CGAAAGTTCC ACTTCCCAAT TTATTACCTT TTAGGAAACA TGGCGGCCGC TGACCTCTTC 
GCTGGCATCT CGTATTCGTA TCTAGTGTTC CACACAGGAC CCTGGACCAT CAATTTGACC 
ATTAAGGAGT GGTTTGTGCG TGGGGCATTG ATTAACATGA GCCTCACCGC CTCGGTGGTC 
AACCTGTTGG CAGTGGCTGT TGAGCGCCAT CAGACCATCT TCACCATGCA GCTCCACACC 
ACGATGACCC GCCGGCGAGT GGTCATGCTG ATAGTCACCA TCTGGGTGGT GGCAGTTGTC 
ATGGGTTTGG TGCCCACCAT GGGCTGGAAT TGTGTGTGTG ACCAGTTAGG TTGCAGCACG 
GTTGCGCCAC TGTACTCGCG CAGCTTCCTA GTGTTTTGGG CCATCCTCAA CCTCATGGCC 
TTCTTCATCA TGGTGGCCGT TTACACGCAC ATCTTCATCT ACGTCCGCCG GCGAAGTCAA 
GTTATGTCCC CTCACACAGA TCAGCCCTCA AATAATCAGA CGGTTATCAG CCTCATGAAG 
ACCATGTCCA TGATACTGGG TGCCTTTGTG ATCTGCTGGA CTCCAGGTCT GGTGATCCTC 
CTTCTGGACG GTCTGAAGTG CATCGAATGT CACGTCCTGA AGTATGAGAA GTACTGCCTG 
GTTCTGGCCG AGTGCAACTC TCTGGTGAAC CCCATCATCT ACTCTTTTCG GGACGAGGAC 
ATGAGGACCA CCTACAAACG CATTCTCTGC TTCCCTTGGA GAAGAATGCG GCCGCACGAG 
GGGCCTTCTG GCATCCGCTT CGAGCCTGTG AAAACAGGGG CTCCCCCTGA GAAAAACAGC 
CAGAACTCCA GCAGCCCAGA GGCCGCTCCT CCACAAAGCC CATTGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298048.1
CDS	316..1386
Translation	MVFNSCEAFDNPVSYFYNRTGKPISPNWRTHDFVVVGLGLPICVFIILANMLVIAAIIVNRKFHFPIYYLLGNMAAADLFAGISYSYLVFHTGPWTINLTIKEWFVRGALINMSLTASVVNLLAVAVERHQTIFTMQLHTTMTRRRVVMLIVTIWVVAVVMGLVPTMGWNCVCDQLGCSTVAPLYSRSFLVFWAILNLMAFFIMVAVYTHIFIYVRRRSQVMSPHTDQPSNNQTVISLMKTMSMILGAFVICWTPGLVILLLDGLKCIECHVLKYEKYCLVLAECNSLVNPIIYSFRDEDMRTTYKRILCFPWRRMRPHEGPSGIRFEPVKTGAPPEKNSQNSSSPEAAPPQSPLS

Target ORF information:

RefSeq Version	XM_009299773.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysophosphatidic acid receptor 2a (lpar2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299773.2

ORF Insert Sequence:

ATGGTATTTA ACAGCTGTGA GGCTTTTGAC AATCCAGTGT CTTACTTCTA CAACAGGACT 
GGCAAGCCTA TTAGTCCAAA CTGGCGCACT CATGACTTTG TGGTGGTCGG TCTGGGGCTC 
CCCATTTGTG TTTTCATCAT CCTGGCCAAT ATGTTGGTCA TAGCGGCTAT AATTGTCAAC 
CGAAAGTTCC ACTTCCCAAT TTATTACCTT TTAGGAAACA TGGCGGCCGC TGACCTCTTC 
GCTGGCATCT CGTATTCGTA TCTAGTGTTC CACACAGGAC CCTGGACCAT CAATTTGACC 
ATTAAGGAGT GGTTTGTGCG TGGGGCATTG ATTAACATGA GCCTCACCGC CTCGGTGGTC 
AACCTGTTGG CAGTGGCTGT TGAGCGCCAT CAGACCATCT TCACCATGCA GCTCCACACC 
ACGATGACCC GCCGGCGAGT GGTCATGCTG ATAGTCACCA TCTGGGTGGT GGCAGTTGTC 
ATGGGTTTGG TGCCCACCAT GGGCTGGAAT TGTGTGTGTG ACCAGTTAGG TTGCAGCACG 
GTTGCGCCAC TGTACTCGCG CAGCTTCCTA GTGTTTTGGG CCATCCTCAA CCTCATGGCC 
TTCTTCATCA TGGTGGCCGT TTACACGCAC ATCTTCATCT ACGTCCGCCG GCGAAGTCAA 
GTTATGTCCC CTCACACAGA TCAGCCCTCA AATAATCAGA CGGTTATCAG CCTCATGAAG 
ACCATGTCCA TGATACTGGG TGCCTTTGTG ATCTGCTGGA CTCCAGGTCT GGTGATCCTC 
CTTCTGGACG GTCTGAAGTG CATCGAATGT CACGTCCTGA AGTATGAGAA GTACTGCCTG 
GTTCTGGCCG AGTGCAACTC TCTGGTGAAC CCCATCATCT ACTCTTTTCG GGACGAGGAC 
ATGAGGACCA CCTACAAACG CATTCTCTGC TTCCCTTGGA GAAGAATGCG GCCGCACGAG 
GGGCCTTCTG GCATCCGCTT CGAGCCTGTG AAAACAGGGG CTCCCCCTGA GAAAAACAGC 
CAGAACTCCA GCAGCCCAGA GGCCGCTCCT CCACAAAGCC CATTGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lpar2a ( XM_009299773.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298048.1 Danio rerio lysophosphatidic acid receptor 2a (lpar2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41138
Clone ID Related Accession (Same CDS sequence)	XM_009299773.2 , XM_009299773.3
Accession Version	XM_009299773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysophosphatidic acid receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTATTTA ACAGCTGTGA GGCTTTTGAC AATCCAGTGT CTTACTTCTA CAACAGGACT 
GGCAAGCCTA TTAGTCCAAA CTGGCGCACT CATGACTTTG TGGTGGTCGG TCTGGGGCTC 
CCCATTTGTG TTTTCATCAT CCTGGCCAAT ATGTTGGTCA TAGCGGCTAT AATTGTCAAC 
CGAAAGTTCC ACTTCCCAAT TTATTACCTT TTAGGAAACA TGGCGGCCGC TGACCTCTTC 
GCTGGCATCT CGTATTCGTA TCTAGTGTTC CACACAGGAC CCTGGACCAT CAATTTGACC 
ATTAAGGAGT GGTTTGTGCG TGGGGCATTG ATTAACATGA GCCTCACCGC CTCGGTGGTC 
AACCTGTTGG CAGTGGCTGT TGAGCGCCAT CAGACCATCT TCACCATGCA GCTCCACACC 
ACGATGACCC GCCGGCGAGT GGTCATGCTG ATAGTCACCA TCTGGGTGGT GGCAGTTGTC 
ATGGGTTTGG TGCCCACCAT GGGCTGGAAT TGTGTGTGTG ACCAGTTAGG TTGCAGCACG 
GTTGCGCCAC TGTACTCGCG CAGCTTCCTA GTGTTTTGGG CCATCCTCAA CCTCATGGCC 
TTCTTCATCA TGGTGGCCGT TTACACGCAC ATCTTCATCT ACGTCCGCCG GCGAAGTCAA 
GTTATGTCCC CTCACACAGA TCAGCCCTCA AATAATCAGA CGGTTATCAG CCTCATGAAG 
ACCATGTCCA TGATACTGGG TGCCTTTGTG ATCTGCTGGA CTCCAGGTCT GGTGATCCTC 
CTTCTGGACG GTCTGAAGTG CATCGAATGT CACGTCCTGA AGTATGAGAA GTACTGCCTG 
GTTCTGGCCG AGTGCAACTC TCTGGTGAAC CCCATCATCT ACTCTTTTCG GGACGAGGAC 
ATGAGGACCA CCTACAAACG CATTCTCTGC TTCCCTTGGA GAAGAATGCG GCCGCACGAG 
GGGCCTTCTG GCATCCGCTT CGAGCCTGTG AAAACAGGGG CTCCCCCTGA GAAAAACAGC 
CAGAACTCCA GCAGCCCAGA GGCCGCTCCT CCACAAAGCC CATTGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298048.1
CDS	314..1384
Translation	MVFNSCEAFDNPVSYFYNRTGKPISPNWRTHDFVVVGLGLPICVFIILANMLVIAAIIVNRKFHFPIYYLLGNMAAADLFAGISYSYLVFHTGPWTINLTIKEWFVRGALINMSLTASVVNLLAVAVERHQTIFTMQLHTTMTRRRVVMLIVTIWVVAVVMGLVPTMGWNCVCDQLGCSTVAPLYSRSFLVFWAILNLMAFFIMVAVYTHIFIYVRRRSQVMSPHTDQPSNNQTVISLMKTMSMILGAFVICWTPGLVILLLDGLKCIECHVLKYEKYCLVLAECNSLVNPIIYSFRDEDMRTTYKRILCFPWRRMRPHEGPSGIRFEPVKTGAPPEKNSQNSSSPEAAPPQSPLS

Target ORF information:

RefSeq Version	XM_009299773.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysophosphatidic acid receptor 2a (lpar2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299773.3

ORF Insert Sequence:

ATGGTATTTA ACAGCTGTGA GGCTTTTGAC AATCCAGTGT CTTACTTCTA CAACAGGACT 
GGCAAGCCTA TTAGTCCAAA CTGGCGCACT CATGACTTTG TGGTGGTCGG TCTGGGGCTC 
CCCATTTGTG TTTTCATCAT CCTGGCCAAT ATGTTGGTCA TAGCGGCTAT AATTGTCAAC 
CGAAAGTTCC ACTTCCCAAT TTATTACCTT TTAGGAAACA TGGCGGCCGC TGACCTCTTC 
GCTGGCATCT CGTATTCGTA TCTAGTGTTC CACACAGGAC CCTGGACCAT CAATTTGACC 
ATTAAGGAGT GGTTTGTGCG TGGGGCATTG ATTAACATGA GCCTCACCGC CTCGGTGGTC 
AACCTGTTGG CAGTGGCTGT TGAGCGCCAT CAGACCATCT TCACCATGCA GCTCCACACC 
ACGATGACCC GCCGGCGAGT GGTCATGCTG ATAGTCACCA TCTGGGTGGT GGCAGTTGTC 
ATGGGTTTGG TGCCCACCAT GGGCTGGAAT TGTGTGTGTG ACCAGTTAGG TTGCAGCACG 
GTTGCGCCAC TGTACTCGCG CAGCTTCCTA GTGTTTTGGG CCATCCTCAA CCTCATGGCC 
TTCTTCATCA TGGTGGCCGT TTACACGCAC ATCTTCATCT ACGTCCGCCG GCGAAGTCAA 
GTTATGTCCC CTCACACAGA TCAGCCCTCA AATAATCAGA CGGTTATCAG CCTCATGAAG 
ACCATGTCCA TGATACTGGG TGCCTTTGTG ATCTGCTGGA CTCCAGGTCT GGTGATCCTC 
CTTCTGGACG GTCTGAAGTG CATCGAATGT CACGTCCTGA AGTATGAGAA GTACTGCCTG 
GTTCTGGCCG AGTGCAACTC TCTGGTGAAC CCCATCATCT ACTCTTTTCG GGACGAGGAC 
ATGAGGACCA CCTACAAACG CATTCTCTGC TTCCCTTGGA GAAGAATGCG GCCGCACGAG 
GGGCCTTCTG GCATCCGCTT CGAGCCTGTG AAAACAGGGG CTCCCCCTGA GAAAAACAGC 
CAGAACTCCA GCAGCCCAGA GGCCGCTCCT CCACAAAGCC CATTGTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish.
Scientific reports637678(2016 Nov)
Frisca F,Colquhoun D,Goldshmit Y,?nk? ML,P?bay A,Kaslin J

Pharmacological activation of lysophosphatidic acid receptors regulates erythropoiesis.
Scientific reports627050(2016 May)
Lin KH,Ho YH,Chiang JC,Li MW,Lin SH,Chen WM,Chiang CL,Lin YN,Yang YJ,Chen CN,Lu J,Huang CJ,Tigyi G,Yao CL,Lee H

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Overexpression of autotaxin, a lysophosphatidic acid-producing enzyme, enhances cardia bifida induced by hypo-sphingosine-1-phosphate signaling in zebrafish embryo.
Journal of biochemistry155(4)235-41(2014 Apr)
Nakanaga K,Hama K,Kano K,Sato T,Yukiura H,Inoue A,Saigusa D,Tokuyama H,Tomioka Y,Nishina H,Kawahara A,Aoki J

Autotaxin regulates vascular development via multiple lysophosphatidic acid (LPA) receptors in zebrafish.
The Journal of biological chemistry286(51)43972-83(2011 Dec)
Yukiura H,Hama K,Nakanaga K,Tanaka M,Asaoka Y,Okudaira S,Arima N,Inoue A,Hashimoto T,Arai H,Kawahara A,Nishina H,Aoki J

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lpar2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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