cpa2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cpa2
Entrez Gene ID	445052
Full Name	carboxypeptidase A2 (pancreatic)
Synonyms	zgc:92530
General protein information	Preferred Names carboxypeptidase A2 (pancreatic) Names carboxypeptidase A2 (pancreatic)
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297897.2	XP_009296172.1	carboxypeptidase A2 (pancreatic) isoform X1
NM_001003446.2	NP_001003446.2	carboxypeptidase A2 (pancreatic) precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cpa2	NP_001013101.2
Gallus gallus (chicken)	CPA2	NP_001244143.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011435	XP_317868.4
Pan troglodytes (chimpanzee)	CPA2	XP_001156440.2
Danio rerio (zebrafish)	cpa2	NP_001003446.1
Drosophila melanogaster (fruit fly)	CG18585	NP_609132.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011434	XP_554784.3
Caenorhabditis elegans (roundworm)	ZC434.9	NP_001251358.1
Caenorhabditis elegans (roundworm)	suro-1	NP_492177.2
	CPA2	XP_001095065.2
Drosophila melanogaster (fruit fly)	CG7025	NP_609133.2
Caenorhabditis elegans (roundworm)	T06A4.1	NP_871809.1
Caenorhabditis elegans (roundworm)	CELE_Y47G6A.19	NP_001021763.1
Caenorhabditis elegans (roundworm)	T06A4.3	NP_001021611.1
Magnaporthe oryzae (rice blast fungus)	MGG_17822	XP_003720328.1
Homo sapiens (human)	CPA2	NP_001860.2
Canis lupus familiaris (dog)	CPA2	XP_532425.2
Mus musculus (house mouse)	Cpa2	NP_001019869.1
Danio rerio (zebrafish)	cpa4	NP_001002217.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008370	XP_001689001.1
Caenorhabditis elegans (roundworm)	Y18H1A.9	NP_490776.2
Caenorhabditis elegans (roundworm)	CELE_W01A8.6	NP_492003.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Antagonistic interactions of hedgehog, Bmp and retinoic acid signals control zebrafish endocrine pancreas development.
Tehrani Z, Lin S
Development (Cambridge, England)138(4)631-40(2011 Feb)

Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding.
Lyons PJ, Ma LH, Baker R, Fricker LD
PloS one5(9)e12967(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cpa2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cpa2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa41112	NM_001003446.2 Latest version!	Danio rerio carboxypeptidase A2 (pancreatic) (cpa2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa41113	XM_009297897.2 Latest version!	Danio rerio carboxypeptidase A2 (pancreatic) (cpa2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cpa2 ( NM_001003446.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001003446.2 Danio rerio carboxypeptidase A2 (pancreatic) (cpa2), mRNA.

Price & Availability↑

CloneID	ODa41112
Clone ID Related Accession (Same CDS sequence)	NM_001003446.2
Accession Version	NM_001003446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	carboxypeptidase A2 (pancreatic) precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR376785.10, BC076057.1, CN322024.1 and AI496886.1. On Aug 22, 2015 this sequence version replaced NM_001003446.1. ##Evidence-Data-START## Transcript exon combination :: BC076057.1, GFIL01007004.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGACTTT GTATAGCAGT CTTTGTGCTT TTGGTGGCTG TTCATTGTGA GGAGCTCTTT 
AATGGAGATC AAGTTCTCAG AATCCATGCA GAATCTAAAG ACCACATCCA AGTCTTGAAG 
GAGCTGGAGA AGGATTCAGG GCTGGATTTC TGGACTCATG GCTTCTCTAC TGAACGTCCT 
GTTGATGTCC GTGTGCCTCA TGCCAGTCTG TATGCCGTCA AAGATTTCCT GAAGGAAAAC 
AACATCTCTT TCTCTGTCAT GATCAACAAT GTTCAGGAGC TTTTGGACAG GGAGAGAGCA 
GAGATGGTTA GTAACACGGA GATGGAGCGC AACACCAAGA GCTTCAACTT TGCTGCTTAT 
CATGATCTGG ATACTATTTA CAGTTTTATG GACACCCTTG TTGCGAGTCA CTCTAATCTG 
ATCTCGAAAG TGAAGATTGG CAGCACCTAT GAGAACCGTC CCATGTATGT CCTGAAGTTC 
AGCACTGGTG GAGAGAATAA ACCTGCGATC TGGATTGATG CTGGGATTCA TGCCAGAGAG 
TGGGTTTCTC ATGCATCTGC TGTGTGGATC GCTGACAGGA TTGCCACAGA CTTTAAGGAA 
AACCGTGCTC TTGCTCGCAA TGTCCTTAGT AAAATGGACA TTTACTTGAT GATTGTGGCC 
AATCCTGATG GATACGTCTT CTCCCACCTC ACCGTCCGGC CGTTTCACCG TTTGTGGCGT 
AAGAGCCGGT CTGTGACCTC TAACCCAAAC TGCCCTGGTG TCGACCTCAA CAGAAACTTT 
GATGCTGAAT TTAGTGGTCC TGGAGCCAGT GATGACCCAT GTGCTGAAGA CTATCGCGGC 
CCTTCTGCTC ATTCAGAGAT TGAAGTGACG AACATTGCGG ATTTCATTTT GAGCCACGGC 
AACTTCAAAT CCTTCATGAC CCTCCATGCT TATTCTCAGC TTCTAATGTA TCCCTACGGC 
TACACCGGCT CAAATGCTCC TGACCAGCCT GACCTTCATG ATGTGGCCAC TCAGGCGAAC 
AAAGCCCTCA ATTCTTGGCA TGGCACCATT TATAAAGTTG GAAGCATCTT CCACACTATT 
GAGCAAGCTA GTGGTGCAAG TGTGGACTGG GCTTACCAAC ATGGCATCAA ATATTGCTTC 
GCTTTTGAGC TGCGTGACAC TGGTGAATAT GGATTCCTCC TGCCCGCTGA CCAGATCGTT 
TCCACTGCAA GGGAGACATG GTTTGCTCTC AGATACATAA TGCAATATGT TTCTGATCAT 
CCTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003446.2
CDS	38..1306
Translation	MRLCIAVFVLLVAVHCEELFNGDQVLRIHAESKDHIQVLKELEKDSGLDFWTHGFSTERPVDVRVPHASLYAVKDFLKENNISFSVMINNVQELLDRERAEMVSNTEMERNTKSFNFAAYHDLDTIYSFMDTLVASHSNLISKVKIGSTYENRPMYVLKFSTGGENKPAIWIDAGIHAREWVSHASAVWIADRIATDFKENRALARNVLSKMDIYLMIVANPDGYVFSHLTVRPFHRLWRKSRSVTSNPNCPGVDLNRNFDAEFSGPGASDDPCAEDYRGPSAHSEIEVTNIADFILSHGNFKSFMTLHAYSQLLMYPYGYTGSNAPDQPDLHDVATQANKALNSWHGTIYKVGSIFHTIEQASGASVDWAYQHGIKYCFAFELRDTGEYGFLLPADQIVSTARETWFALRYIMQYVSDHPY

Target ORF information:

RefSeq Version	NM_001003446.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxypeptidase A2 (pancreatic) (cpa2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003446.2

ORF Insert Sequence:

ATGAGACTTT GTATAGCAGT CTTTGTGCTT TTGGTGGCTG TTCATTGTGA GGAGCTCTTT 
AATGGAGATC AAGTTCTCAG AATCCATGCA GAATCTAAAG ACCACATCCA AGTCTTGAAG 
GAGCTGGAGA AGGATTCAGG GCTGGATTTC TGGACTCATG GCTTCTCTAC TGAACGTCCT 
GTTGATGTCC GTGTGCCTCA TGCCAGTCTG TATGCCGTCA AAGATTTCCT GAAGGAAAAC 
AACATCTCTT TCTCTGTCAT GATCAACAAT GTTCAGGAGC TTTTGGACAG GGAGAGAGCA 
GAGATGGTTA GTAACACGGA GATGGAGCGC AACACCAAGA GCTTCAACTT TGCTGCTTAT 
CATGATCTGG ATACTATTTA CAGTTTTATG GACACCCTTG TTGCGAGTCA CTCTAATCTG 
ATCTCGAAAG TGAAGATTGG CAGCACCTAT GAGAACCGTC CCATGTATGT CCTGAAGTTC 
AGCACTGGTG GAGAGAATAA ACCTGCGATC TGGATTGATG CTGGGATTCA TGCCAGAGAG 
TGGGTTTCTC ATGCATCTGC TGTGTGGATC GCTGACAGGA TTGCCACAGA CTTTAAGGAA 
AACCGTGCTC TTGCTCGCAA TGTCCTTAGT AAAATGGACA TTTACTTGAT GATTGTGGCC 
AATCCTGATG GATACGTCTT CTCCCACCTC ACCGTCCGGC CGTTTCACCG TTTGTGGCGT 
AAGAGCCGGT CTGTGACCTC TAACCCAAAC TGCCCTGGTG TCGACCTCAA CAGAAACTTT 
GATGCTGAAT TTAGTGGTCC TGGAGCCAGT GATGACCCAT GTGCTGAAGA CTATCGCGGC 
CCTTCTGCTC ATTCAGAGAT TGAAGTGACG AACATTGCGG ATTTCATTTT GAGCCACGGC 
AACTTCAAAT CCTTCATGAC CCTCCATGCT TATTCTCAGC TTCTAATGTA TCCCTACGGC 
TACACCGGCT CAAATGCTCC TGACCAGCCT GACCTTCATG ATGTGGCCAC TCAGGCGAAC 
AAAGCCCTCA ATTCTTGGCA TGGCACCATT TATAAAGTTG GAAGCATCTT CCACACTATT 
GAGCAAGCTA GTGGTGCAAG TGTGGACTGG GCTTACCAAC ATGGCATCAA ATATTGCTTC 
GCTTTTGAGC TGCGTGACAC TGGTGAATAT GGATTCCTCC TGCCCGCTGA CCAGATCGTT 
TCCACTGCAA GGGAGACATG GTTTGCTCTC AGATACATAA TGCAATATGT TTCTGATCAT 
CCTTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cpa2 ( XM_009297897.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296172.1 Danio rerio carboxypeptidase A2 (pancreatic) (cpa2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41113
Clone ID Related Accession (Same CDS sequence)	XM_009297897.2
Accession Version	XM_009297897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	carboxypeptidase A2 (pancreatic) isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAAA GAGTCACATT AACCTGCATC AGCACTAATA TTACTGAGAA CTCAACACAT 
GTGTCTGAGA TTATAAAAGC TGATCTCAAT GAAAACTCCA GACAGTGGAG ATCATTTCTA 
CAACTGGAGA ACATGAGACT TTGTATAGCA GTCTTTGTGC TTTTGGTGGC TGTTCATTGT 
GAGGAGCTCT TTAATGGAGA TCAAGTTCTC AGAATCCATG CAGAATCTAA AGACCACATC 
CAAGTCTTGA AGGAGCTGGA GAAGGATTCA GGGCTGGATT TCTGGACTCA TGGCTTCTCT 
ACTGAACGTC CTGTTGATGT CCGTGTGCCT CATGCCAGTC TGTATGCCGT CAAAGATTTC 
CTGAAGGAAA ACAACATCTC TTTCTCTGTC ATGATCAACA ATGTTCAGGA GCTTTTGGAC 
AGGGAGAGAG CAGAGATGGT TAGTAACACG GAGATGGAGC GCAACACCAA GAGCTTCAAC 
TTTGCTGCTT ATCATGATCT GGATACTATT TACAGTTTTA TGGACACCCT TGTTGCGAGT 
CACTCTAATC TGATCTCGAA AGTGAAGATT GGCAGCACCT ATGAGAACCG TCCCATGTAT 
GTCCTGAAGT TCAGCACTGG TGGAGAGAAT AAACCTGCGA TCTGGATTGA TGCTGGGATT 
CATGCCAGAG AGTGGGTTTC TCATGCATCT GCTGTGTGGA TCGCTGACAG GATTGCCACA 
GACTTTAAGG AAAACCGTGC TCTTGCTCGC AATGTCCTTA GTAAAATGGA CATTTACTTG 
ATGATTGTGG CCAATCCTGA TGGATACGTC TTCTCCCACC TCACCGTCCG GGTCCTGGAG 
CCAGTGATGA CCCATGTGCT GAAGACTATC GCGGCCCTTC TGCTCATTCA GAGATTGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296172.1
CDS	213..1115
Translation	MVKRVTLTCISTNITENSTHVSEIIKADLNENSRQWRSFLQLENMRLCIAVFVLLVAVHCEELFNGDQVLRIHAESKDHIQVLKELEKDSGLDFWTHGFSTERPVDVRVPHASLYAVKDFLKENNISFSVMINNVQELLDRERAEMVSNTEMERNTKSFNFAAYHDLDTIYSFMDTLVASHSNLISKVKIGSTYENRPMYVLKFSTGGENKPAIWIDAGIHAREWVSHASAVWIADRIATDFKENRALARNVLSKMDIYLMIVANPDGYVFSHLTVRVLEPVMTHVLKTIAALLLIQRLK

Target ORF information:

RefSeq Version	XM_009297897.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carboxypeptidase A2 (pancreatic) (cpa2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297897.2

ORF Insert Sequence:

ATGGTGAAAA GAGTCACATT AACCTGCATC AGCACTAATA TTACTGAGAA CTCAACACAT 
GTGTCTGAGA TTATAAAAGC TGATCTCAAT GAAAACTCCA GACAGTGGAG ATCATTTCTA 
CAACTGGAGA ACATGAGACT TTGTATAGCA GTCTTTGTGC TTTTGGTGGC TGTTCATTGT 
GAGGAGCTCT TTAATGGAGA TCAAGTTCTC AGAATCCATG CAGAATCTAA AGACCACATC 
CAAGTCTTGA AGGAGCTGGA GAAGGATTCA GGGCTGGATT TCTGGACTCA TGGCTTCTCT 
ACTGAACGTC CTGTTGATGT CCGTGTGCCT CATGCCAGTC TGTATGCCGT CAAAGATTTC 
CTGAAGGAAA ACAACATCTC TTTCTCTGTC ATGATCAACA ATGTTCAGGA GCTTTTGGAC 
AGGGAGAGAG CAGAGATGGT TAGTAACACG GAGATGGAGC GCAACACCAA GAGCTTCAAC 
TTTGCTGCTT ATCATGATCT GGATACTATT TACAGTTTTA TGGACACCCT TGTTGCGAGT 
CACTCTAATC TGATCTCGAA AGTGAAGATT GGCAGCACCT ATGAGAACCG TCCCATGTAT 
GTCCTGAAGT TCAGCACTGG TGGAGAGAAT AAACCTGCGA TCTGGATTGA TGCTGGGATT 
CATGCCAGAG AGTGGGTTTC TCATGCATCT GCTGTGTGGA TCGCTGACAG GATTGCCACA 
GACTTTAAGG AAAACCGTGC TCTTGCTCGC AATGTCCTTA GTAAAATGGA CATTTACTTG 
ATGATTGTGG CCAATCCTGA TGGATACGTC TTCTCCCACC TCACCGTCCG GGTCCTGGAG 
CCAGTGATGA CCCATGTGCT GAAGACTATC GCGGCCCTTC TGCTCATTCA GAGATTGAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Antagonistic interactions of hedgehog, Bmp and retinoic acid signals control zebrafish endocrine pancreas development.
Development (Cambridge, England)138(4)631-40(2011 Feb)
Tehrani Z,Lin S

Carboxypeptidase A6 in zebrafish development and implications for VIth cranial nerve pathfinding.
PloS one5(9)e12967(2010 Sep)
Lyons PJ,Ma LH,Baker R,Fricker LD