thap12b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	thap12b
Entrez Gene ID	436953
Full Name	THAP domain containing 12b
Synonyms	prkrir,prkrirb,zgc:86870
General protein information	Preferred Names THAP domain containing 12b Names 52 kDa repressor of the inhibitor of the protein kinase protein-kinase, interferon-inducible double stranded RNA dependent inhibitor, repressor of (P58 repressor) b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002680.1	NP_001002680.1	52 kDa repressor of the inhibitor of the protein kinase

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Prkrir	NP_001178559.1
Gallus gallus (chicken)	PRKRIR	XP_425679.3
Pan troglodytes (chimpanzee)	PRKRIR	XP_003313264.1
	PRKRIR	XP_001086430.1
Bos taurus (cattle)	PRKRIR	NP_001179132.1
Mus musculus (house mouse)	Prkrir	NP_082686.1
Danio rerio (zebrafish)	prkrirb	NP_001002680.1
Homo sapiens (human)	PRKRIR	NP_004696.2
Canis lupus familiaris (dog)	PRKRIR	XP_542300.2
Xenopus tropicalis (tropical clawed frog)	prkrir	NP_989053.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The THAP domain of THAP1 is a large C2CH module with zinc-dependent sequence-specific DNA-binding activity.
Clouaire T, Roussigne M, Ecochard V, Mathe C, Amalric F, Girard JP
Proceedings of the National Academy of Sciences of the United States of America102(19)6907-12(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following thap12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thap12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa31513	NM_001002680.1 Latest version!	Danio rerio THAP domain containing 12b (thap12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thap12b ( NM_001002680.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002680.1 Danio rerio THAP domain containing 12b (thap12b), mRNA.

Price & Availability↑

CloneID	ODa31513
Clone ID Related Accession (Same CDS sequence)	NM_001002680.1
Accession Version	NM_001002680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	52 kDa repressor of the inhibitor of the protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC075781.1. ##Evidence-Data-START## Transcript exon combination :: BC075781.1, CK683948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCAAATT TTTGTGCCGC TCCGAACTGT ACTAGGAAAA GCACCCAATC CGATTTAGCT 
TTCTTCAGGT TTCCACGGGA TCCAGAGAGA TGTAGGCTGT GGGTGGAGAA CTGTCGGCGT 
GCAGATCTGG AGGAGAAAAC CCCTGATCAA CTGAACAAGC ACTACCGACT GTGTGCTAAA 
CACTTTGAAC CAGCCATGAT TTGCAAAACT AGTCCATATA GGACAGTACT AAGAGACACT 
GCTATACCCA CAATTTTTGA TTTAACCAGT CATCTAGGCA ACCCCCACAG CAGACATCGC 
AAACGCATCA AAGTTTTGAC AGATGAAGAG GTACAGAAAA TCAAGGAAAG GCGATTGGAG 
TCTTCAATTG AACAACTCTC GAAAAAAGAC AGTGATGCTC AGGATGACAG CGAGACCACC 
GATGATGTAC CTCGGCTCAC GCCCGAGCAG AAAGACCTAA GAGAGTTCTT AAAGTCTTCA 
TTTGAAGTCA TGATTTTGAT AGGAAGGCAA AGCTTTCCAT ACAGCAGCAA TGCAAGAGAA 
GATGAAATGC GCTCTAAGGA GGAATGGCTG TTTAATCCAG GTAACTTTCA AGCATTGCTT 
GAGAACCGCA TCAATGCAGG TGATGAGTTT CTCAGAAGGA GATTTGAGTC AGCGGCCGTT 
AATGAGGAAT ATTGTCCAGC CGTCCAGCAG AGACAGCTTC TGGATGTCTG TGAGAGATGC 
GTCCGTGAAG AGATCCTTCA GCAGGTCAGG GAATGCCGCT TTTTTTCGAT TGTCACCGGT 
GAGTTGGTGG ACTTCCCAGA GGGAGAGCAT CTACCTATAT TCCTGCGCTT CGTAGACCAA 
GACAACACTC TCCGGGAAGA GTTCATCGAG TTCCTCTCAT TCGAAGGAGA GGAGGTCACT 
GTTGCTGAGA GATTAGAGTC TCGCTTGATG AAGGGTTGGG GCTTGAGTAT GGAGCACTGC 
CGAGGACAAG CCTACGCAGG ATCTGGTATA CTTGGCACCA AGATGAAGGT CATCACTTCC 
CAGCTAAAAG AAAAATATCC CATGGCGGTG ATCACACCTA CTTCCACCTG TGCTCTTAAT 
GTTCATCTTG CTAACGGCAT GTCGTTGACC GGAGTGCAAG TCGTGATGTC AGTTTTAAAG 
AAGTCAAATG CTTTTTTCAG AGCATCCCCT TTGCTACAGA CTGAACTTGA CAATGCCATC 
TCCATCCTCT GCCAGGGAAA CCTAGAAAAA GAAAGCATCC TCAAAGAGGG CTCTCGATCA 
ACCTGGACAG ATCTTCATAA CCTGTTTGAG ATGGCTGTGG ATTTGTTGGA GCCACTTCTG 
CTCTGCATGG ACAGCATACA TGATAATGAG GATCTAAAAT GGAGTGACCA AGTCACCAGT 
GACGCTTACG CCATTTCCGA GGCCTTGGCG GACTTTGAGT TTATTGTGAC GTTGGTTATT 
TTAAAGAACG CATTGTCTTT TACCAGAGCG TTTGGGAAGA ACTTGCAAGG AGAAACCATT 
GATGTGTTTT TCGCCGCCAA CAGTTTAACT GCAGTCCTGC ATTCGCTGCA TGAGGTATCA 
GACAATCTGG AGGTATACCA TGAATTCTGG TTCGAAGAGG CTGTCAATAT GGCGACTGCA 
ATGGAAGTCC CGGTGAGAGT GCCAAGGTTG TTTCTTCGGA AGCAACAAAC GCCGGACACT 
ATAGAAATAC AGGCAGAGTC GTTCTTTAAA GAGTACCTCA CACAGCCAGT TATGGAGTAC 
ATCACGCAGG AGGTCAAGGA CATCTTCTCT GAAAATCATC TCAAAGCGCT CAAGTGCCTG 
TCACTCGTTC CTGCAGTCAT GGGCCAAATG AGGTTCAATA CCTCTGAGGA GAGCCACGCG 
GACATTTACA GGACCGATCT ACCCCATCCT GACACGCTGC CTGCTGAACT GCATTGTTGG 
AGGATAAAAT GGAAACACAG AGGCAAGGAA GTTAGCTTGC CGTCCACCAT TTACGAAACC 
CTTCAACACT CCGATGTGAA GTTCTTCCCA AATGTTAATG CGTTTTTGAA AATATTAGCC 
ACATTACCTG TGCTGAAGCT TGATGGGAGT CACGGTGAAA CTGCACAAAG ACGCTTACAA 
GCTTACCTTA CAGACACTGC GGTCAGCCAG CGGTGTAAAA ATCTGGCGGT GCTAAATATC 
AATTACCATA TAAAGACAGA TCTTGAAGAA ATGGTCCAGT GTTATTTAAA ACGTTACCCA 
GAGGAAAGCA AGACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002680.1
CDS	69..2309
Translation	MPNFCAAPNCTRKSTQSDLAFFRFPRDPERCRLWVENCRRADLEEKTPDQLNKHYRLCAKHFEPAMICKTSPYRTVLRDTAIPTIFDLTSHLGNPHSRHRKRIKVLTDEEVQKIKERRLESSIEQLSKKDSDAQDDSETTDDVPRLTPEQKDLREFLKSSFEVMILIGRQSFPYSSNAREDEMRSKEEWLFNPGNFQALLENRINAGDEFLRRRFESAAVNEEYCPAVQQRQLLDVCERCVREEILQQVRECRFFSIVTGELVDFPEGEHLPIFLRFVDQDNTLREEFIEFLSFEGEEVTVAERLESRLMKGWGLSMEHCRGQAYAGSGILGTKMKVITSQLKEKYPMAVITPTSTCALNVHLANGMSLTGVQVVMSVLKKSNAFFRASPLLQTELDNAISILCQGNLEKESILKEGSRSTWTDLHNLFEMAVDLLEPLLLCMDSIHDNEDLKWSDQVTSDAYAISEALADFEFIVTLVILKNALSFTRAFGKNLQGETIDVFFAANSLTAVLHSLHEVSDNLEVYHEFWFEEAVNMATAMEVPVRVPRLFLRKQQTPDTIEIQAESFFKEYLTQPVMEYITQEVKDIFSENHLKALKCLSLVPAVMGQMRFNTSEESHADIYRTDLPHPDTLPAELHCWRIKWKHRGKEVSLPSTIYETLQHSDVKFFPNVNAFLKILATLPVLKLDGSHGETAQRRLQAYLTDTAVSQRCKNLAVLNINYHIKTDLEEMVQCYLKRYPEESKTD

Target ORF information:

RefSeq Version	NM_001002680.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio THAP domain containing 12b (thap12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002680.1

ORF Insert Sequence:

ATGCCAAATT TTTGTGCCGC TCCGAACTGT ACTAGGAAAA GCACCCAATC CGATTTAGCT 
TTCTTCAGGT TTCCACGGGA TCCAGAGAGA TGTAGGCTGT GGGTGGAGAA CTGTCGGCGT 
GCAGATCTGG AGGAGAAAAC CCCTGATCAA CTGAACAAGC ACTACCGACT GTGTGCTAAA 
CACTTTGAAC CAGCCATGAT TTGCAAAACT AGTCCATATA GGACAGTACT AAGAGACACT 
GCTATACCCA CAATTTTTGA TTTAACCAGT CATCTAGGCA ACCCCCACAG CAGACATCGC 
AAACGCATCA AAGTTTTGAC AGATGAAGAG GTACAGAAAA TCAAGGAAAG GCGATTGGAG 
TCTTCAATTG AACAACTCTC GAAAAAAGAC AGTGATGCTC AGGATGACAG CGAGACCACC 
GATGATGTAC CTCGGCTCAC GCCCGAGCAG AAAGACCTAA GAGAGTTCTT AAAGTCTTCA 
TTTGAAGTCA TGATTTTGAT AGGAAGGCAA AGCTTTCCAT ACAGCAGCAA TGCAAGAGAA 
GATGAAATGC GCTCTAAGGA GGAATGGCTG TTTAATCCAG GTAACTTTCA AGCATTGCTT 
GAGAACCGCA TCAATGCAGG TGATGAGTTT CTCAGAAGGA GATTTGAGTC AGCGGCCGTT 
AATGAGGAAT ATTGTCCAGC CGTCCAGCAG AGACAGCTTC TGGATGTCTG TGAGAGATGC 
GTCCGTGAAG AGATCCTTCA GCAGGTCAGG GAATGCCGCT TTTTTTCGAT TGTCACCGGT 
GAGTTGGTGG ACTTCCCAGA GGGAGAGCAT CTACCTATAT TCCTGCGCTT CGTAGACCAA 
GACAACACTC TCCGGGAAGA GTTCATCGAG TTCCTCTCAT TCGAAGGAGA GGAGGTCACT 
GTTGCTGAGA GATTAGAGTC TCGCTTGATG AAGGGTTGGG GCTTGAGTAT GGAGCACTGC 
CGAGGACAAG CCTACGCAGG ATCTGGTATA CTTGGCACCA AGATGAAGGT CATCACTTCC 
CAGCTAAAAG AAAAATATCC CATGGCGGTG ATCACACCTA CTTCCACCTG TGCTCTTAAT 
GTTCATCTTG CTAACGGCAT GTCGTTGACC GGAGTGCAAG TCGTGATGTC AGTTTTAAAG 
AAGTCAAATG CTTTTTTCAG AGCATCCCCT TTGCTACAGA CTGAACTTGA CAATGCCATC 
TCCATCCTCT GCCAGGGAAA CCTAGAAAAA GAAAGCATCC TCAAAGAGGG CTCTCGATCA 
ACCTGGACAG ATCTTCATAA CCTGTTTGAG ATGGCTGTGG ATTTGTTGGA GCCACTTCTG 
CTCTGCATGG ACAGCATACA TGATAATGAG GATCTAAAAT GGAGTGACCA AGTCACCAGT 
GACGCTTACG CCATTTCCGA GGCCTTGGCG GACTTTGAGT TTATTGTGAC GTTGGTTATT 
TTAAAGAACG CATTGTCTTT TACCAGAGCG TTTGGGAAGA ACTTGCAAGG AGAAACCATT 
GATGTGTTTT TCGCCGCCAA CAGTTTAACT GCAGTCCTGC ATTCGCTGCA TGAGGTATCA 
GACAATCTGG AGGTATACCA TGAATTCTGG TTCGAAGAGG CTGTCAATAT GGCGACTGCA 
ATGGAAGTCC CGGTGAGAGT GCCAAGGTTG TTTCTTCGGA AGCAACAAAC GCCGGACACT 
ATAGAAATAC AGGCAGAGTC GTTCTTTAAA GAGTACCTCA CACAGCCAGT TATGGAGTAC 
ATCACGCAGG AGGTCAAGGA CATCTTCTCT GAAAATCATC TCAAAGCGCT CAAGTGCCTG 
TCACTCGTTC CTGCAGTCAT GGGCCAAATG AGGTTCAATA CCTCTGAGGA GAGCCACGCG 
GACATTTACA GGACCGATCT ACCCCATCCT GACACGCTGC CTGCTGAACT GCATTGTTGG 
AGGATAAAAT GGAAACACAG AGGCAAGGAA GTTAGCTTGC CGTCCACCAT TTACGAAACC 
CTTCAACACT CCGATGTGAA GTTCTTCCCA AATGTTAATG CGTTTTTGAA AATATTAGCC 
ACATTACCTG TGCTGAAGCT TGATGGGAGT CACGGTGAAA CTGCACAAAG ACGCTTACAA 
GCTTACCTTA CAGACACTGC GGTCAGCCAG CGGTGTAAAA ATCTGGCGGT GCTAAATATC 
AATTACCATA TAAAGACAGA TCTTGAAGAA ATGGTCCAGT GTTATTTAAA ACGTTACCCA 
GAGGAAAGCA AGACTGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The THAP domain of THAP1 is a large C2CH module with zinc-dependent sequence-specific DNA-binding activity.
Proceedings of the National Academy of Sciences of the United States of America102(19)6907-12(2005 May)
Clouaire T,Roussigne M,Ecochard V,Mathe C,Amalric F,Girard JP

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thap12b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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