atp2b3a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005166818.4	XP_005166875.1	plasma membrane calcium ATPase 3a isoform X1
XM_005166819.4	XP_005166876.1	plasma membrane calcium ATPase 3a isoform X2
XM_021478240.1	XP_021333915.1	plasma membrane calcium ATPase 3a isoform X7
XM_017357316.2	XP_017212805.1	plasma membrane calcium ATPase 3a isoform X5
NM_001002472.3	NP_001002472.2	plasma membrane calcium ATPase 3a isoform 2
XM_005166819.3	XP_005166876.1	plasma membrane calcium ATPase 3a isoform X2
NM_001002472.2	NP_001002472.2	plasma membrane calcium ATPase 3a
XM_017357315.1	XP_017212804.1	plasma membrane calcium ATPase 3a isoform X4
XM_017357316.1	XP_017212805.1	plasma membrane calcium ATPase 3a isoform X5
XM_005166822.3	XP_005166879.1	plasma membrane calcium ATPase 3a isoform X6
XM_017357317.1	XP_017212806.1	plasma membrane calcium ATPase 3a isoform X7
XM_021478239.1	XP_021333914.1	plasma membrane calcium ATPase 3a isoform X4
NM_001365433.1	NP_001352362.1	plasma membrane calcium ATPase 3a isoform 1
XM_005166818.3	XP_005166875.1	plasma membrane calcium ATPase 3a isoform X1
XM_005166820.3	XP_005166877.1	plasma membrane calcium ATPase 3a isoform X3
XM_005166820.4	XP_005166877.1	plasma membrane calcium ATPase 3a isoform X3
XM_005166822.4	XP_005166879.1	plasma membrane calcium ATPase 3a isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59958	NM_001365433.1 Latest version!	Danio rerio ATPase plasma membrane Ca2+ transporting 3a (atp2b3a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa59958	XM_017357316.2 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa13968	NM_001002472.3 Latest version!	Danio rerio ATPase plasma membrane Ca2+ transporting 3a (atp2b3a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13963	XM_005166818.4 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13964	XM_005166819.4 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13965	XM_005166820.4 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13966	XM_021478239.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13966	XM_017357315.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13967	XM_005166822.4 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13969	XM_017357317.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13969	XM_021478240.1 Latest version!	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa13964	XM_005166819.3	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13968	NM_001002472.2	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa59958	XM_017357316.1	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa13963	XM_005166818.3	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13965	XM_005166820.3	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa13967	XM_005166822.3	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

atp2b3a ( NM_001365433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352362.1 Danio rerio ATPase plasma membrane Ca2+ transporting 3a (atp2b3a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa59958
Clone ID Related Accession (Same CDS sequence)	XM_017357316.2 , XM_017357316.1 , NM_001365433.1
Accession Version	NM_001365433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX248385.6. On Aug 24, 2018 this sequence version replaced XM_017357316.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01001888.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAGTGAGTCC TTACATTCCG TTAACGGCAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352362.1
CDS	982..4578
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGSESLHSVNGTS

Target ORF information:

RefSeq Version	NM_001365433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase plasma membrane Ca2+ transporting 3a (atp2b3a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365433.1

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAGTGAGTCC TTACATTCCG TTAACGGCAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_017357316.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212805.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa59958
Clone ID Related Accession (Same CDS sequence)	XM_017357316.2 , XM_017357316.1 , NM_001365433.1
Accession Version	XM_017357316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357316.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAGTGAGTCC TTACATTCCG TTAACGGCAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212805.1
CDS	903..4499
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGSESLHSVNGTS

Target ORF information:

RefSeq Version	XM_017357316.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357316.2

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAGTGAGTCC TTACATTCCG TTAACGGCAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_017357316.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212805.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X5, mRNA.

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CloneID	ODa59958
Clone ID Related Accession (Same CDS sequence)	XM_017357316.2 , XM_017357316.1 , NM_001365433.1
Accession Version	XM_017357316.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAGTGAGTCC TTACATTCCG TTAACGGCAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212805.1
CDS	989..4585
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGSESLHSVNGTS

Target ORF information:

RefSeq Version	XM_017357316.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357316.1

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAGTGAGTCC TTACATTCCG TTAACGGCAC CTCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( NM_001002472.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002472.2 Danio rerio ATPase plasma membrane Ca2+ transporting 3a (atp2b3a), transcript variant 2, mRNA.

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CloneID	ODa13968
Clone ID Related Accession (Same CDS sequence)	NM_001002472.3 , NM_001002472.2
Accession Version	NM_001002472.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX248385.6. On Aug 24, 2018 this sequence version replaced NM_001002472.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: EU559283.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAGTGA GTCCTTACAT TCCGTTAACG GCACCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002472.2
CDS	982..4512
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGSESLHSVNGTS

Target ORF information:

RefSeq Version	NM_001002472.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase plasma membrane Ca2+ transporting 3a (atp2b3a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002472.3

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAGTGA GTCCTTACAT TCCGTTAACG GCACCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( NM_001002472.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002472.2 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), mRNA.

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CloneID	ODa13968
Clone ID Related Accession (Same CDS sequence)	NM_001002472.3 , NM_001002472.2
Accession Version	NM_001002472.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU559283.1. On Dec 3, 2010 this sequence version replaced NM_001002472.1. ##Evidence-Data-START## Transcript exon combination :: EU559283.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAGTGA GTCCTTACAT TCCGTTAACG GCACCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002472.2
CDS	331..3861
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGSESLHSVNGTS

Target ORF information:

RefSeq Version	NM_001002472.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002472.2

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAGTGA GTCCTTACAT TCCGTTAACG GCACCTCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166818.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166875.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X1, mRNA.

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CloneID	ODa13963
Clone ID Related Accession (Same CDS sequence)	XM_005166818.4 , XM_005166818.3
Accession Version	XM_005166818.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166818.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACATCCG GGTGGTGAAA GCTTTCCGTA GCTCCCTGTA CGACGGCATC 
GAGCGGCCCG AGTCGCGCAA CTCCATTCAC GACTTCCAGG CGCACCCGGA GTTCATCATC 
ACGGACTCGG TCCACAACAT CCCCCTGATC GACGAGACGG ATGTGGACGA CGAATCTGAG 
CGCTCCAATC ATAACCACGT GCGCGTGGCT CTCCGACACC CTCACTCTCA ACCCCAGGCG 
CCCCAGAGGC CCCCTCGATC CCGCTATCCA TCCCGTCCAC TAAGGCAGCA GAGTCTGCCG 
GTCACCCTCA ACTGCAACAA CAACGCCGCC GTCGAAAGCA GAGTCCACCT GGGCACGAAC 
GGCAGCGGGG CGCCACACAC TCCTGCCAGC CCCCTGCACA GCCTGGAGAC CTGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166875.1
CDS	901..4800
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_005166818.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166818.4

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACATCCG GGTGGTGAAA GCTTTCCGTA GCTCCCTGTA CGACGGCATC 
GAGCGGCCCG AGTCGCGCAA CTCCATTCAC GACTTCCAGG CGCACCCGGA GTTCATCATC 
ACGGACTCGG TCCACAACAT CCCCCTGATC GACGAGACGG ATGTGGACGA CGAATCTGAG 
CGCTCCAATC ATAACCACGT GCGCGTGGCT CTCCGACACC CTCACTCTCA ACCCCAGGCG 
CCCCAGAGGC CCCCTCGATC CCGCTATCCA TCCCGTCCAC TAAGGCAGCA GAGTCTGCCG 
GTCACCCTCA ACTGCAACAA CAACGCCGCC GTCGAAAGCA GAGTCCACCT GGGCACGAAC 
GGCAGCGGGG CGCCACACAC TCCTGCCAGC CCCCTGCACA GCCTGGAGAC CTGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166818.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166875.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa13963
Clone ID Related Accession (Same CDS sequence)	XM_005166818.4 , XM_005166818.3
Accession Version	XM_005166818.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166818.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACATCCG GGTGGTGAAA GCTTTCCGTA GCTCCCTGTA CGACGGCATC 
GAGCGGCCCG AGTCGCGCAA CTCCATTCAC GACTTCCAGG CGCACCCGGA GTTCATCATC 
ACGGACTCGG TCCACAACAT CCCCCTGATC GACGAGACGG ATGTGGACGA CGAATCTGAG 
CGCTCCAATC ATAACCACGT GCGCGTGGCT CTCCGACACC CTCACTCTCA ACCCCAGGCG 
CCCCAGAGGC CCCCTCGATC CCGCTATCCA TCCCGTCCAC TAAGGCAGCA GAGTCTGCCG 
GTCACCCTCA ACTGCAACAA CAACGCCGCC GTCGAAAGCA GAGTCCACCT GGGCACGAAC 
GGCAGCGGGG CGCCACACAC TCCTGCCAGC CCCCTGCACA GCCTGGAGAC CTGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166875.1
CDS	987..4886
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_005166818.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166818.3

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACATCCG GGTGGTGAAA GCTTTCCGTA GCTCCCTGTA CGACGGCATC 
GAGCGGCCCG AGTCGCGCAA CTCCATTCAC GACTTCCAGG CGCACCCGGA GTTCATCATC 
ACGGACTCGG TCCACAACAT CCCCCTGATC GACGAGACGG ATGTGGACGA CGAATCTGAG 
CGCTCCAATC ATAACCACGT GCGCGTGGCT CTCCGACACC CTCACTCTCA ACCCCAGGCG 
CCCCAGAGGC CCCCTCGATC CCGCTATCCA TCCCGTCCAC TAAGGCAGCA GAGTCTGCCG 
GTCACCCTCA ACTGCAACAA CAACGCCGCC GTCGAAAGCA GAGTCCACCT GGGCACGAAC 
GGCAGCGGGG CGCCACACAC TCCTGCCAGC CCCCTGCACA GCCTGGAGAC CTGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166819.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166876.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa13964
Clone ID Related Accession (Same CDS sequence)	XM_005166819.4 , XM_005166819.3
Accession Version	XM_005166819.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166819.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACA TCCGGGTGGT GAAAGCTTTC CGTAGCTCCC TGTACGACGG CATCGAGCGG 
CCCGAGTCGC GCAACTCCAT TCACGACTTC CAGGCGCACC CGGAGTTCAT CATCACGGAC 
TCGGTCCACA ACATCCCCCT GATCGACGAG ACGGATGTGG ACGACGAATC TGAGCGCTCC 
AATCATAACC ACGTGCGCGT GGCTCTCCGA CACCCTCACT CTCAACCCCA GGCGCCCCAG 
AGGCCCCCTC GATCCCGCTA TCCATCCCGT CCACTAAGGC AGCAGAGTCT GCCGGTCACC 
CTCAACTGCA ACAACAACGC CGCCGTCGAA AGCAGAGTCC ACCTGGGCAC GAACGGCAGC 
GGGGCGCCAC ACACTCCTGC CAGCCCCCTG CACAGCCTGG AGACCTGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166876.1
CDS	900..4733
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_005166819.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166819.4

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACA TCCGGGTGGT GAAAGCTTTC CGTAGCTCCC TGTACGACGG CATCGAGCGG 
CCCGAGTCGC GCAACTCCAT TCACGACTTC CAGGCGCACC CGGAGTTCAT CATCACGGAC 
TCGGTCCACA ACATCCCCCT GATCGACGAG ACGGATGTGG ACGACGAATC TGAGCGCTCC 
AATCATAACC ACGTGCGCGT GGCTCTCCGA CACCCTCACT CTCAACCCCA GGCGCCCCAG 
AGGCCCCCTC GATCCCGCTA TCCATCCCGT CCACTAAGGC AGCAGAGTCT GCCGGTCACC 
CTCAACTGCA ACAACAACGC CGCCGTCGAA AGCAGAGTCC ACCTGGGCAC GAACGGCAGC 
GGGGCGCCAC ACACTCCTGC CAGCCCCCTG CACAGCCTGG AGACCTGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166819.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166876.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X2, mRNA.

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CloneID	ODa13964
Clone ID Related Accession (Same CDS sequence)	XM_005166819.4 , XM_005166819.3
Accession Version	XM_005166819.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166819.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACA TCCGGGTGGT GAAAGCTTTC CGTAGCTCCC TGTACGACGG CATCGAGCGG 
CCCGAGTCGC GCAACTCCAT TCACGACTTC CAGGCGCACC CGGAGTTCAT CATCACGGAC 
TCGGTCCACA ACATCCCCCT GATCGACGAG ACGGATGTGG ACGACGAATC TGAGCGCTCC 
AATCATAACC ACGTGCGCGT GGCTCTCCGA CACCCTCACT CTCAACCCCA GGCGCCCCAG 
AGGCCCCCTC GATCCCGCTA TCCATCCCGT CCACTAAGGC AGCAGAGTCT GCCGGTCACC 
CTCAACTGCA ACAACAACGC CGCCGTCGAA AGCAGAGTCC ACCTGGGCAC GAACGGCAGC 
GGGGCGCCAC ACACTCCTGC CAGCCCCCTG CACAGCCTGG AGACCTGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166876.1
CDS	987..4820
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_005166819.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166819.3

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACA TCCGGGTGGT GAAAGCTTTC CGTAGCTCCC TGTACGACGG CATCGAGCGG 
CCCGAGTCGC GCAACTCCAT TCACGACTTC CAGGCGCACC CGGAGTTCAT CATCACGGAC 
TCGGTCCACA ACATCCCCCT GATCGACGAG ACGGATGTGG ACGACGAATC TGAGCGCTCC 
AATCATAACC ACGTGCGCGT GGCTCTCCGA CACCCTCACT CTCAACCCCA GGCGCCCCAG 
AGGCCCCCTC GATCCCGCTA TCCATCCCGT CCACTAAGGC AGCAGAGTCT GCCGGTCACC 
CTCAACTGCA ACAACAACGC CGCCGTCGAA AGCAGAGTCC ACCTGGGCAC GAACGGCAGC 
GGGGCGCCAC ACACTCCTGC CAGCCCCCTG CACAGCCTGG AGACCTGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166820.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166877.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13965
Clone ID Related Accession (Same CDS sequence)	XM_005166820.3 , XM_005166820.4
Accession Version	XM_005166820.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166820.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT CCGGGTGGTG 
AAAGCTTTCC GTAGCTCCCT GTACGACGGC ATCGAGCGGC CCGAGTCGCG CAACTCCATT 
CACGACTTCC AGGCGCACCC GGAGTTCATC ATCACGGACT CGGTCCACAA CATCCCCCTG 
ATCGACGAGA CGGATGTGGA CGACGAATCT GAGCGCTCCA ATCATAACCA CGTGCGCGTG 
GCTCTCCGAC ACCCTCACTC TCAACCCCAG GCGCCCCAGA GGCCCCCTCG ATCCCGCTAT 
CCATCCCGTC CACTAAGGCA GCAGAGTCTG CCGGTCACCC TCAACTGCAA CAACAACGCC 
GCCGTCGAAA GCAGAGTCCA CCTGGGCACG AACGGCAGCG GGGCGCCACA CACTCCTGCC 
AGCCCCCTGC ACAGCCTGGA GACCTGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166877.1
CDS	987..4799
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_005166820.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166820.3

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT CCGGGTGGTG 
AAAGCTTTCC GTAGCTCCCT GTACGACGGC ATCGAGCGGC CCGAGTCGCG CAACTCCATT 
CACGACTTCC AGGCGCACCC GGAGTTCATC ATCACGGACT CGGTCCACAA CATCCCCCTG 
ATCGACGAGA CGGATGTGGA CGACGAATCT GAGCGCTCCA ATCATAACCA CGTGCGCGTG 
GCTCTCCGAC ACCCTCACTC TCAACCCCAG GCGCCCCAGA GGCCCCCTCG ATCCCGCTAT 
CCATCCCGTC CACTAAGGCA GCAGAGTCTG CCGGTCACCC TCAACTGCAA CAACAACGCC 
GCCGTCGAAA GCAGAGTCCA CCTGGGCACG AACGGCAGCG GGGCGCCACA CACTCCTGCC 
AGCCCCCTGC ACAGCCTGGA GACCTGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166820.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166877.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13965
Clone ID Related Accession (Same CDS sequence)	XM_005166820.3 , XM_005166820.4
Accession Version	XM_005166820.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166820.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT CCGGGTGGTG 
AAAGCTTTCC GTAGCTCCCT GTACGACGGC ATCGAGCGGC CCGAGTCGCG CAACTCCATT 
CACGACTTCC AGGCGCACCC GGAGTTCATC ATCACGGACT CGGTCCACAA CATCCCCCTG 
ATCGACGAGA CGGATGTGGA CGACGAATCT GAGCGCTCCA ATCATAACCA CGTGCGCGTG 
GCTCTCCGAC ACCCTCACTC TCAACCCCAG GCGCCCCAGA GGCCCCCTCG ATCCCGCTAT 
CCATCCCGTC CACTAAGGCA GCAGAGTCTG CCGGTCACCC TCAACTGCAA CAACAACGCC 
GCCGTCGAAA GCAGAGTCCA CCTGGGCACG AACGGCAGCG GGGCGCCACA CACTCCTGCC 
AGCCCCCTGC ACAGCCTGGA GACCTGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166877.1
CDS	901..4713
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_005166820.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166820.4

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT CCGGGTGGTG 
AAAGCTTTCC GTAGCTCCCT GTACGACGGC ATCGAGCGGC CCGAGTCGCG CAACTCCATT 
CACGACTTCC AGGCGCACCC GGAGTTCATC ATCACGGACT CGGTCCACAA CATCCCCCTG 
ATCGACGAGA CGGATGTGGA CGACGAATCT GAGCGCTCCA ATCATAACCA CGTGCGCGTG 
GCTCTCCGAC ACCCTCACTC TCAACCCCAG GCGCCCCAGA GGCCCCCTCG ATCCCGCTAT 
CCATCCCGTC CACTAAGGCA GCAGAGTCTG CCGGTCACCC TCAACTGCAA CAACAACGCC 
GCCGTCGAAA GCAGAGTCCA CCTGGGCACG AACGGCAGCG GGGCGCCACA CACTCCTGCC 
AGCCCCCTGC ACAGCCTGGA GACCTGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_017357315.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212804.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X4, mRNA.

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CloneID	ODa13966
Clone ID Related Accession (Same CDS sequence)	XM_017357315.1 , XM_021478239.1
Accession Version	XM_017357315.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATCCGGGT GGTGAAAGCT 
TTCCGTAGCT CCCTGTACGA CGGCATCGAG CGGCCCGAGT CGCGCAACTC CATTCACGAC 
TTCCAGGCGC ACCCGGAGTT CATCATCACG GACTCGGTCC ACAACATCCC CCTGATCGAC 
GAGACGGATG TGGACGACGA ATCTGAGCGC TCCAATCATA ACCACGTGCG CGTGGCTCTC 
CGACACCCTC ACTCTCAACC CCAGGCGCCC CAGAGGCCCC CTCGATCCCG CTATCCATCC 
CGTCCACTAA GGCAGCAGAG TCTGCCGGTC ACCCTCAACT GCAACAACAA CGCCGCCGTC 
GAAAGCAGAG TCCACCTGGG CACGAACGGC AGCGGGGCGC CACACACTCC TGCCAGCCCC 
CTGCACAGCC TGGAGACCTG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212804.1
CDS	1..3747
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_017357315.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357315.1

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATCCGGGT GGTGAAAGCT 
TTCCGTAGCT CCCTGTACGA CGGCATCGAG CGGCCCGAGT CGCGCAACTC CATTCACGAC 
TTCCAGGCGC ACCCGGAGTT CATCATCACG GACTCGGTCC ACAACATCCC CCTGATCGAC 
GAGACGGATG TGGACGACGA ATCTGAGCGC TCCAATCATA ACCACGTGCG CGTGGCTCTC 
CGACACCCTC ACTCTCAACC CCAGGCGCCC CAGAGGCCCC CTCGATCCCG CTATCCATCC 
CGTCCACTAA GGCAGCAGAG TCTGCCGGTC ACCCTCAACT GCAACAACAA CGCCGCCGTC 
GAAAGCAGAG TCCACCTGGG CACGAACGGC AGCGGGGCGC CACACACTCC TGCCAGCCCC 
CTGCACAGCC TGGAGACCTG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_021478239.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333914.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X4, mRNA.

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CloneID	ODa13966
Clone ID Related Accession (Same CDS sequence)	XM_017357315.1 , XM_021478239.1
Accession Version	XM_021478239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATCCGGGT GGTGAAAGCT 
TTCCGTAGCT CCCTGTACGA CGGCATCGAG CGGCCCGAGT CGCGCAACTC CATTCACGAC 
TTCCAGGCGC ACCCGGAGTT CATCATCACG GACTCGGTCC ACAACATCCC CCTGATCGAC 
GAGACGGATG TGGACGACGA ATCTGAGCGC TCCAATCATA ACCACGTGCG CGTGGCTCTC 
CGACACCCTC ACTCTCAACC CCAGGCGCCC CAGAGGCCCC CTCGATCCCG CTATCCATCC 
CGTCCACTAA GGCAGCAGAG TCTGCCGGTC ACCCTCAACT GCAACAACAA CGCCGCCGTC 
GAAAGCAGAG TCCACCTGGG CACGAACGGC AGCGGGGCGC CACACACTCC TGCCAGCCCC 
CTGCACAGCC TGGAGACCTG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333914.1
CDS	900..4646
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYDGIERPESRNSIHDFQAHPEFIITDSVHNIPLIDETDVDDESERSNHNHVRVALRHPHSQPQAPQRPPRSRYPSRPLRQQSLPVTLNCNNNAAVESRVHLGTNGSGAPHTPASPLHSLETCL

Target ORF information:

RefSeq Version	XM_021478239.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478239.1

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATCCGGGT GGTGAAAGCT 
TTCCGTAGCT CCCTGTACGA CGGCATCGAG CGGCCCGAGT CGCGCAACTC CATTCACGAC 
TTCCAGGCGC ACCCGGAGTT CATCATCACG GACTCGGTCC ACAACATCCC CCTGATCGAC 
GAGACGGATG TGGACGACGA ATCTGAGCGC TCCAATCATA ACCACGTGCG CGTGGCTCTC 
CGACACCCTC ACTCTCAACC CCAGGCGCCC CAGAGGCCCC CTCGATCCCG CTATCCATCC 
CGTCCACTAA GGCAGCAGAG TCTGCCGGTC ACCCTCAACT GCAACAACAA CGCCGCCGTC 
GAAAGCAGAG TCCACCTGGG CACGAACGGC AGCGGGGCGC CACACACTCC TGCCAGCCCC 
CTGCACAGCC TGGAGACCTG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166822.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166879.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa13967
Clone ID Related Accession (Same CDS sequence)	XM_005166822.3 , XM_005166822.4
Accession Version	XM_005166822.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAATCCGGGT GGTGAAAGCT TTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166879.1
CDS	989..4576
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGNPGGESFP

Target ORF information:

RefSeq Version	XM_005166822.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166822.3

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAATCCGGGT GGTGAAAGCT TTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_005166822.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166879.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X6, mRNA.

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CloneID	ODa13967
Clone ID Related Accession (Same CDS sequence)	XM_005166822.3 , XM_005166822.4
Accession Version	XM_005166822.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166822.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAATCCGGGT GGTGAAAGCT TTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166879.1
CDS	903..4490
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKEVNSNSSTQFPSVEAKEHNIINGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGNPGGESFP

Target ORF information:

RefSeq Version	XM_005166822.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166822.4

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGA GGTCAATAGT AATTCATCCA CGCAGTTCCC CAGTGTTGAA 
GCCAAAGAAC ACAACATCAT AAATGGGAAA CAAGATGGAA CCCTGGAGAA CAACCAAAAT 
AAAGCTAAGA AACAGGATGA GGCTGTTGCC ATGGAGATGC AGCCTCTGAA GAGTGCAGAA 
GGTGGTGAAG TGGAGGAGAA AGAGAAGAAA AAAGCCAGTG TGCCCAAGAA GGAGAAATCC 
GTTCTTCAGG GCAAACTCAC CAAGCTGGCA GTGCAAATTG GGAAAGCAGG TCTGGTGATG 
TCGGCCATCA CTGTGATTAT CTTGATGCTG TACTTTGTGA TTAAAACGTT CATTGTTCAT 
AAACAGCCAT GGCTGACAGA GTGCACACCG ATCTACGTGC AATACTTTGT CAAGTTCTTC 
ATCATTGGAG TTACAGTTCT GGTAGTGGCA GTGCCTGAGG GTCTGCCGCT GGCTGTCACC 
ATATCACTGG CCTACTCCGT AAAAAAAATG ATGAAGGATA ATAACCTTGT GCGTCATTTG 
GATGCCTGTG AGACTATGGG CAATGCCACG GCTATTTGCT CTGATAAAAC AGGGACCCTC 
ACCACTAACC GGATGACAGT AGTCCAGATT TATATAGGGG ACCAGCTCTT TCGGGACATC 
CCAACGCCAG ACCAGATTAA CCCAAGGACT TTGGAGCTCA TCAGCAGCGC TATTGCTGTG 
AACTGCGCCT ACACCTCCAA AATAATGCCT GCTGATAAGG AAGGTGGGCT GCCCAAACAG 
GTGGGTAATA AGACAGAGTG TGCTCTGCTG GGACTCGTCC TGGACCTGAA GCAGGACTAC 
CAAGCTGTGA GAGAGCAGAT CCCAGAGGAG TTACTCTATA AAGTCTACAC CTTTAATTCA 
GTCCGAAAAT CCATGAGCAC CGTCATTCAG ATGCCTGATG GAAGCTTCCG CTTGTACAGC 
AAAGGAGCCT CTGAGATCCT GCTCAAAAAA TGCTCGTTCA TTTTATCGCG TGACGGCGAG 
GCTCGTGCTT TTAGGGCACG GGACAAAGAC GAAATGGTAA AGAAAGTGAT TGAGCCGATG 
GCATGTGATG GCCTTCGTAC GATTTGCATT GCATACCGGG AGCTGCCAGC TGACCCCCTG 
CCAGACTGGG ACAATGAGAC CGACATCGTC TCCAACCTTA CCTGCATCAC TGTGGTCGGC 
ATTGAAGACC CTGTTCGACC AGAGGTCCCA GATGCCATCA GAAAGTGCCA GCGAGCAGGC 
ATCACTGTGC GTATGGTGAC GGGTGACAAT ATAAACACTG CACGTGCCAT TGCTGCCAAG 
TGTGGCATCA TCCATCCTGG CGACGACTTC CTGTGTATGG AAGGAAAGGA CTTCAATAGG 
CGCATCAGGA ATGAGAAGGG AGAGATTGAG CAGGAGCGCA TTGATAAAAT TTGGCCCAAG 
CTCAGAGTTC TTGCTCGCTC CTCCCCGACT GACAAACACA CTCTAGTTAA AGGTATTATA 
GACAGCACTG TTGTGGAACA AAGGCAGGTG GTTGCAGTCA CTGGTGACGG CACAAATGAT 
GGGCCTGCCC TGAAGAAAGC TGATGTGGGG TTTGCAATGG GCATTGCTGG TACAGATGTG 
GCCAAGGAGG CGTCTGACAT CATCCTGACC GATGATAACT TCTCCAGCAT AGTGAAGGCT 
GTGATGTGGG GACGAAACGT GTATGACAGC ATCTCCAAGT TTCTGCAGTT CCAGCTCACA 
GTGAATGTGG TGGCTGTTAT AGTAGCCTTC ACCGGCGCCT GCATCACACA GGATTCACCC 
CTGAAAGCTG TGCAGATGCT TTGGGTCAAT CTGATCATGG ACACATTTGC TTCTCTTGCT 
CTTGCTACGG AGCCGCCAAC GGAAGCTCTG CTCCTGAGGA AGCCTTATGG CCGAAACAAC 
CCTCTCATCT CCAGAACGAT GATGAAGAAC ATCCTTGGAC ATGCCGTCTA CCAGCTCGTC 
ATCATCTTTA CCTTGCTCTT CGCCGGTGAG AGGATCTTTG ACATTGACAG CGGCCGTGAT 
GCTCCGCTTC ACTCTCCTCC ATCTGAGCAC TACACCATCA TCTTTAACAC GTTTGTCCTC 
ATGCAACTCT TCAACGAGAT CAATGCCCGT AAGATTCACG GAGAGAGGAA CGTGTTCGAT 
GGGATATTCT CAAACCCCAT ATTCTGTTCG ATCGTGCTGG GTACATTTGC AATACAGATC 
GTGATTGTTC AGTTTGGAGG GAAGCCCTTC AGCTGTTCCC CCTTAAACGT GGAGCAGTGG 
CTCTGGTGTC TGTTTGTGGG AATGGGAGAG CTGCTCTGGG GTCAGTTGAT TTCTTCAGTG 
CCAACGCATC AGCTGAAGTG TCTGAAAGAA GCGGGCCACG GGCCGGCTCC AGACGAGATG 
CTGGATGAAG ATCTGGCCGA GGACGAGGAG GAGATTGATT ATGCTGAGAG AGAGCTGAGA 
CGAGGACAGA TCCTGTGGTT TAGAGGACTC AACCGCATTC AGACTCAGAT GGAGGTAGTG 
AGTACCTTCA AGAGAAGTGG TTCGTTTCAG GGTGCAGTGA GACGCCGCTC TTCAGTGCTC 
AGCCAACTCC ATGACGTAAC CAATAATTCT ACTCCCTCTC ACGTAGTTCT CTCCACTGCC 
AATGCAACTG GTGCCCCCGG GAATCCGGGT GGTGAAAGCT TTCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_021478240.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333915.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X7, mRNA.

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CloneID	ODa13969
Clone ID Related Accession (Same CDS sequence)	XM_021478240.1 , XM_017357317.1
Accession Version	XM_021478240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAATCC GGGTGGTGAA AGCTTTCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333915.1
CDS	902..4423
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGNPGGESFP

Target ORF information:

RefSeq Version	XM_021478240.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478240.1

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAATCC GGGTGGTGAA AGCTTTCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

atp2b3a ( XM_017357317.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212806.1 Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X7, mRNA.

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CloneID	ODa13969
Clone ID Related Accession (Same CDS sequence)	XM_021478240.1 , XM_017357317.1
Accession Version	XM_017357317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	plasma membrane calcium ATPase 3a isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAATCC GGGTGGTGAA AGCTTTCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212806.1
CDS	1..3522
Translation	MGDLGNSTVEFHPKKPGMDQGGHDGDFGVSVDELCSLMELRGAEALQKIQENYTNTETLCHRLKTSPADGLSDNPADLEKRRKVFGMNFIPPKQPKTFLELVWEALQDITLIILEIAAIISLGLSFYQPPGGDSEACVEVSEGAEDEGEADANWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQRFAVVRNSTVIQIPVAEMVVGDIAQVKYGDLLPADGVLIQGNDLKIDESSLTGESDHVRKSIDKDPMLLSGTHVMEGSGKMLVTAVGVNSQTGIIFTLLGAGEMEEEKKDCKKGKQDGTLENNQNKAKKQDEAVAMEMQPLKSAEGGEVEEKEKKKASVPKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILMLYFVIKTFIVHKQPWLTECTPIYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQIYIGDQLFRDIPTPDQINPRTLELISSAIAVNCAYTSKIMPADKEGGLPKQVGNKTECALLGLVLDLKQDYQAVREQIPEELLYKVYTFNSVRKSMSTVIQMPDGSFRLYSKGASEILLKKCSFILSRDGEARAFRARDKDEMVKKVIEPMACDGLRTICIAYRELPADPLPDWDNETDIVSNLTCITVVGIEDPVRPEVPDAIRKCQRAGITVRMVTGDNINTARAIAAKCGIIHPGDDFLCMEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTVVEQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTEALLLRKPYGRNNPLISRTMMKNILGHAVYQLVIIFTLLFAGERIFDIDSGRDAPLHSPPSEHYTIIFNTFVLMQLFNEINARKIHGERNVFDGIFSNPIFCSIVLGTFAIQIVIVQFGGKPFSCSPLNVEQWLWCLFVGMGELLWGQLISSVPTHQLKCLKEAGHGPAPDEMLDEDLAEDEEEIDYAERELRRGQILWFRGLNRIQTQMEVVSTFKRSGSFQGAVRRRSSVLSQLHDVTNNSTPSHVVLSTANATGAPGNPGGESFP

Target ORF information:

RefSeq Version	XM_017357317.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ATPase, Ca++ transporting, plasma membrane 3a (atp2b3a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357317.1

ORF Insert Sequence:

ATGGGGGATC TGGGCAATAG CACAGTAGAG TTCCACCCTA AAAAGCCAGG AATGGACCAG 
GGAGGCCATG ATGGGGATTT TGGGGTGTCG GTGGATGAGT TGTGCTCACT GATGGAGCTC 
AGGGGAGCCG AGGCCCTGCA GAAGATCCAG GAGAACTACA CAAATACAGA AACCCTTTGT 
CACAGACTCA AGACTTCACC TGCAGATGGG CTATCGGACA ATCCTGCGGA TCTGGAGAAA 
CGTCGAAAGG TTTTTGGAAT GAACTTCATC CCTCCTAAGC AGCCCAAGAC CTTCCTTGAG 
CTTGTGTGGG AGGCTCTACA GGATATTACC CTCATAATCC TGGAAATAGC GGCCATTATT 
TCCCTCGGAC TGTCCTTTTA CCAGCCGCCA GGAGGGGACA GTGAAGCATG TGTTGAAGTG 
AGTGAAGGTG CGGAGGACGA GGGAGAGGCG GATGCCAACT GGATTGAGGG TGCAGCTATC 
CTGCTATCGG TGATCTGCGT GGTGCTGGTG ACTGCCTTCA ATGACTGGAG CAAAGAAAAG 
CAGTTTCGTG GCTTGCAGAG CCGCATCGAA CAGGAGCAAC GTTTCGCTGT GGTGCGCAAC 
AGCACGGTCA TTCAAATCCC TGTGGCTGAG ATGGTGGTGG GGGATATTGC CCAGGTCAAA 
TATGGCGACC TCCTGCCTGC GGATGGTGTT CTCATCCAAG GGAACGACCT AAAGATCGAT 
GAGAGCTCCC TCACTGGAGA GTCTGATCAC GTACGCAAAT CCATTGACAA GGATCCCATG 
CTGCTGTCAG GCACTCATGT AATGGAGGGG TCAGGGAAAA TGTTGGTGAC TGCAGTTGGC 
GTCAACTCTC AAACGGGAAT CATTTTCACC CTACTGGGGG CCGGAGAAAT GGAGGAGGAG 
AAGAAAGACT GCAAGAAAGG GAAACAAGAT GGAACCCTGG AGAACAACCA AAATAAAGCT 
AAGAAACAGG ATGAGGCTGT TGCCATGGAG ATGCAGCCTC TGAAGAGTGC AGAAGGTGGT 
GAAGTGGAGG AGAAAGAGAA GAAAAAAGCC AGTGTGCCCA AGAAGGAGAA ATCCGTTCTT 
CAGGGCAAAC TCACCAAGCT GGCAGTGCAA ATTGGGAAAG CAGGTCTGGT GATGTCGGCC 
ATCACTGTGA TTATCTTGAT GCTGTACTTT GTGATTAAAA CGTTCATTGT TCATAAACAG 
CCATGGCTGA CAGAGTGCAC ACCGATCTAC GTGCAATACT TTGTCAAGTT CTTCATCATT 
GGAGTTACAG TTCTGGTAGT GGCAGTGCCT GAGGGTCTGC CGCTGGCTGT CACCATATCA 
CTGGCCTACT CCGTAAAAAA AATGATGAAG GATAATAACC TTGTGCGTCA TTTGGATGCC 
TGTGAGACTA TGGGCAATGC CACGGCTATT TGCTCTGATA AAACAGGGAC CCTCACCACT 
AACCGGATGA CAGTAGTCCA GATTTATATA GGGGACCAGC TCTTTCGGGA CATCCCAACG 
CCAGACCAGA TTAACCCAAG GACTTTGGAG CTCATCAGCA GCGCTATTGC TGTGAACTGC 
GCCTACACCT CCAAAATAAT GCCTGCTGAT AAGGAAGGTG GGCTGCCCAA ACAGGTGGGT 
AATAAGACAG AGTGTGCTCT GCTGGGACTC GTCCTGGACC TGAAGCAGGA CTACCAAGCT 
GTGAGAGAGC AGATCCCAGA GGAGTTACTC TATAAAGTCT ACACCTTTAA TTCAGTCCGA 
AAATCCATGA GCACCGTCAT TCAGATGCCT GATGGAAGCT TCCGCTTGTA CAGCAAAGGA 
GCCTCTGAGA TCCTGCTCAA AAAATGCTCG TTCATTTTAT CGCGTGACGG CGAGGCTCGT 
GCTTTTAGGG CACGGGACAA AGACGAAATG GTAAAGAAAG TGATTGAGCC GATGGCATGT 
GATGGCCTTC GTACGATTTG CATTGCATAC CGGGAGCTGC CAGCTGACCC CCTGCCAGAC 
TGGGACAATG AGACCGACAT CGTCTCCAAC CTTACCTGCA TCACTGTGGT CGGCATTGAA 
GACCCTGTTC GACCAGAGGT CCCAGATGCC ATCAGAAAGT GCCAGCGAGC AGGCATCACT 
GTGCGTATGG TGACGGGTGA CAATATAAAC ACTGCACGTG CCATTGCTGC CAAGTGTGGC 
ATCATCCATC CTGGCGACGA CTTCCTGTGT ATGGAAGGAA AGGACTTCAA TAGGCGCATC 
AGGAATGAGA AGGGAGAGAT TGAGCAGGAG CGCATTGATA AAATTTGGCC CAAGCTCAGA 
GTTCTTGCTC GCTCCTCCCC GACTGACAAA CACACTCTAG TTAAAGGTAT TATAGACAGC 
ACTGTTGTGG AACAAAGGCA GGTGGTTGCA GTCACTGGTG ACGGCACAAA TGATGGGCCT 
GCCCTGAAGA AAGCTGATGT GGGGTTTGCA ATGGGCATTG CTGGTACAGA TGTGGCCAAG 
GAGGCGTCTG ACATCATCCT GACCGATGAT AACTTCTCCA GCATAGTGAA GGCTGTGATG 
TGGGGACGAA ACGTGTATGA CAGCATCTCC AAGTTTCTGC AGTTCCAGCT CACAGTGAAT 
GTGGTGGCTG TTATAGTAGC CTTCACCGGC GCCTGCATCA CACAGGATTC ACCCCTGAAA 
GCTGTGCAGA TGCTTTGGGT CAATCTGATC ATGGACACAT TTGCTTCTCT TGCTCTTGCT 
ACGGAGCCGC CAACGGAAGC TCTGCTCCTG AGGAAGCCTT ATGGCCGAAA CAACCCTCTC 
ATCTCCAGAA CGATGATGAA GAACATCCTT GGACATGCCG TCTACCAGCT CGTCATCATC 
TTTACCTTGC TCTTCGCCGG TGAGAGGATC TTTGACATTG ACAGCGGCCG TGATGCTCCG 
CTTCACTCTC CTCCATCTGA GCACTACACC ATCATCTTTA ACACGTTTGT CCTCATGCAA 
CTCTTCAACG AGATCAATGC CCGTAAGATT CACGGAGAGA GGAACGTGTT CGATGGGATA 
TTCTCAAACC CCATATTCTG TTCGATCGTG CTGGGTACAT TTGCAATACA GATCGTGATT 
GTTCAGTTTG GAGGGAAGCC CTTCAGCTGT TCCCCCTTAA ACGTGGAGCA GTGGCTCTGG 
TGTCTGTTTG TGGGAATGGG AGAGCTGCTC TGGGGTCAGT TGATTTCTTC AGTGCCAACG 
CATCAGCTGA AGTGTCTGAA AGAAGCGGGC CACGGGCCGG CTCCAGACGA GATGCTGGAT 
GAAGATCTGG CCGAGGACGA GGAGGAGATT GATTATGCTG AGAGAGAGCT GAGACGAGGA 
CAGATCCTGT GGTTTAGAGG ACTCAACCGC ATTCAGACTC AGATGGAGGT AGTGAGTACC 
TTCAAGAGAA GTGGTTCGTT TCAGGGTGCA GTGAGACGCC GCTCTTCAGT GCTCAGCCAA 
CTCCATGACG TAACCAATAA TTCTACTCCC TCTCACGTAG TTCTCTCCAC TGCCAATGCA 
ACTGGTGCCC CCGGGAATCC GGGTGGTGAA AGCTTTCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	atp2b3a
Entrez Gene ID	436745
Full Name	ATPase, Ca++ transporting, plasma membrane 3a
Synonyms	atp2b3,pmca3a,wu:fk54d07,zgc:92885
General protein information	Preferred Names ATPase, Ca++ transporting, plasma membrane 3a Names plasma membrane calcium ATPase 3a ATPase, Ca++ transporting, plasma membrane 3 plasma membrane calcium ATPase 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Caenorhabditis elegans (roundworm)	mca-3	NP_001255244.1
Rattus norvegicus (Norway rat)	Atp2b3	NP_579822.1
Danio rerio (zebrafish)	atp2b3b	NP_001121714.1
Drosophila melanogaster (fruit fly)	PMCA	NP_001188518.1
Magnaporthe oryzae (rice blast fungus)	MGG_07971	XP_003713218.1
Canis lupus familiaris (dog)	ATP2B3	XP_003435597.1
Danio rerio (zebrafish)	atp2b3a	NP_001002472.2
Caenorhabditis elegans (roundworm)	mca-2	NP_500161.1
Oryza sativa (rice)	Os03g0616400	NP_001050661.1
Homo sapiens (human)	ATP2B3	NP_001001344.1
Bos taurus (cattle)	ATP2B3	NP_001178093.1
Mus musculus (house mouse)	Atp2b3	NP_796210.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010638	XP_311357.4
Xenopus tropicalis (tropical clawed frog)	atp2b3	NP_001124515.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

atp2b3a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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