ift81 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ift81
Entrez Gene ID	432390
Full Name	intraflagellar transport 81 homolog
Synonyms	cdv1
General protein information	Preferred Names intraflagellar transport 81 homolog Names intraflagellar transport protein 81 homolog carnitine deficiency-associated gene expressed in ventricle 1 larry
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002313.1	NP_001002313.1	intraflagellar transport protein 81 homolog

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002293	XP_307884.3
Pan troglodytes (chimpanzee)	IFT81	XP_001141289.1
Rattus norvegicus (Norway rat)	Ift81	NP_954551.1
Danio rerio (zebrafish)	ift81	NP_001002313.1
Bos taurus (cattle)	IFT81	NP_001074994.1
Mus musculus (house mouse)	Ift81	NP_034009.2
Xenopus tropicalis (tropical clawed frog)	ift81	XP_002937744.2
Homo sapiens (human)	IFT81	NP_001137251.1
	IFT81	XP_001099060.2
Canis lupus familiaris (dog)	IFT81	XP_003639956.1
Gallus gallus (chicken)	IFT81	NP_001239238.1
Caenorhabditis elegans (roundworm)	ift-81	NP_508900.6

Gene Ontology
Bibliography

Related articles in PubMed

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Ribas L, Liew WC, D?az N, Sreenivasan R, Orb?n L, Piferrer F
Proceedings of the National Academy of Sciences of the United States of America114(6)E941-E950(2017 Feb)

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Shim H, Kim JH, Kim CY, Hwang S, Kim H, Yang S, Lee JE, Lee I
Nucleic acids research44(20)9611-9623(2016 Nov)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish Tsc1 reveals functional interactions between the cilium and the TOR pathway.
DiBella LM, Park A, Sun Z
Human molecular genetics18(4)595-606(2009 Feb)

A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney.
Sun Z, Amsterdam A, Pazour GJ, Cole DG, Miller MS, Hopkins N
Development (Cambridge, England)131(16)4085-93(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ift81 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ift81 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34298	NM_001002313.1 Latest version!	Danio rerio intraflagellar transport 81 homolog (ift81), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ift81 ( NM_001002313.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002313.1 Danio rerio intraflagellar transport 81 homolog (ift81), mRNA.

Price & Availability↑

CloneID	ODa34298
Clone ID Related Accession (Same CDS sequence)	NM_001002313.1
Accession Version	NM_001002313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	intraflagellar transport protein 81 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY618922.1. ##Evidence-Data-START## Transcript exon combination :: AY618922.1, BC163882.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGAAC AGCTTAAATT TATCGTTGAA CAGCTGAACA AGGAGCCTTT CAAGAAAAAC 
TTCAATCTCA TTACATTCGA CTCTCTGGAG CCAATGCAGC TTCTGCAGAC TTTAAGTGAT 
GTTCTGGCTG AAATTGATCC AAAGCAAGCC ATTGATATAC GTGAGGAATT GCCTGAGCAG 
ACAGCCAAGA GAATGTTCAC TCTTCTGGGA ATGCTGAAAT ACAAACCTTC TGGAGGAATG 
TCTGAAGTAA GCAGTTTTCG TCAGGGTTTG GTGTCGGGCA GTAAGCCTGT GGTGCACCCC 
ATCCTGCACT GGCTGTTACA GAGGATTCCA GAGCTGAAGA AGAGAGCGTA TCTGGCCAGA 
TTCCTCGTCA AACTAGAGGT TCCTGCTGAG TTTCTGCAAG ATGATGTCAT TGCTGAGACC 
TACCACCAGT ATGAGGAGCT GGTGGAGGGC TTCAAGAACA TCCACAAAGA GTGTGAACAG 
TTAAAGAGTT CTGGGTTCTC CACTGCTGAG ATTCGACGGG ACATTGTTGC AATGGAGGAG 
GAGAAGGATC AGCTCATCAA AAGAGTGGAA CGTCTTAGAA AAAGGGTGGA GGCAGTGGCA 
AACCACCAGC GCATGCTGGA CCTGGCCAGA CAGTTAAGAG TGGAAAGAGA AAGAGAGGAA 
TCACTCGCTC ATCAGAAACA GGAGCAGAAA AACCAGCTCT TCCAGGCAGA GCAGAGACTG 
CAGAGATGTC AGATCCAGCT CAAAGACTTA CAGCAGGCAG CAGCAGACGA AAAGCCAGAG 
AGCCTTATGA AGAGACTTGA GGAGGACATT AAGTTCAACT CCTACATGGT GTCTGAGAAG 
CTCCCCAGAG AGTTGGAGAA TATGAGAAAG GTGGTGCAGT ACCTACAGAA GGTGGCGTCT 
GAGCCTGCTA TGGGCCAGGC AGAACTGAGG GAGCTTGAGG ACAAGATAAG AGAAACCAAC 
ACTGAGATCA ATCATTTGAT TGAAAAGAGG ATGATGCGGA ACGACCCAAT GGATGATAAA 
CTCTCTCTTT TCAGGCAGCA GGCCGCTATT ATAGTCCGTA AGAAGGAAAC AAAGGTGGAG 
GAGTTGCAGG AGGCCCGTGA GGAGCTGGCG GCAGTGGAAA GAGAGCTGAA CATGAAGACC 
AGTCAGGCAC AAGAGCGAGG AGGAGAGCTG ATCCGTGGAG ATGAGTTTAA GAGATATGTG 
GCCAAGATGC GTGGTAAGAG CAGCACATAT AAGAAAAAGC GTCAGGAGAT AGCTGAGCTG 
AAGGCAGAGT ATGGTGTGCT ACAGAGAACC GAAGAGATCC TGAGAGAACG ACACACAGCT 
GGACAACAGC AGCTGCAATC TCTGGAGGCT CAGCGGGGAA TCTCAGGCTA CAGCGATACT 
CAGGAGGAGT TGGAGAGAGT TTCTGCTATC AAGAGCGAGC TGGATGAGAT GAAGGGAAGA 
ACACTGGATG ACATGTCTGA AATGGTAAAG AAGCTGAATT CAGTGATAGC AGAGAAGAAG 
TCTGCTCTAT CTCCGCTGAT AAAGGACCTG AGAGCTCTAA GACAGGAACA CGCGGAGCTC 
GCACCAGAGT ACGAGGAGAA AAAGGCTCAG TATGAAACCT GTGCTGCAGG ACTCGAGAGC 
AACAGATCCA CGCTGGAACA GGAAGTGAGA ACACTCAGGG AAGAGACGGC ACAGGAAGAA 
AGCCGATATC ATCACATTAA CTGCGAGAGA GAGATCATTG AGAGTCAAAT TCAGAGAGCG 
GCAGAACAAA GCAAGATCAG TCAGTCAATG GACGTACAGG TGCGGAGGAC GGCTCTCAGA 
GAGAAGTACA TTGCAAACAC AGCAGAGCAG GAGTCCTTGG GCAAGGTTCT CCGTCAGCAG 
GTGAAGCAGG TGCGAGAGAA CCAGGAGCCC AATATGAGAC AGATGAAGAT GTGGAAGGAT 
CTGGAAACGC TGCTGGAGTG CAAGAAACAG TGCTACCTCA AGGCTCAGAG CCAGGCGCCT 
ATTGGGCACG TCATCCAGGA TGTTGGCAAG GATATGCTGG TTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002313.1
CDS	274..2301
Translation	MSEQLKFIVEQLNKEPFKKNFNLITFDSLEPMQLLQTLSDVLAEIDPKQAIDIREELPEQTAKRMFTLLGMLKYKPSGGMSEVSSFRQGLVSGSKPVVHPILHWLLQRIPELKKRAYLARFLVKLEVPAEFLQDDVIAETYHQYEELVEGFKNIHKECEQLKSSGFSTAEIRRDIVAMEEEKDQLIKRVERLRKRVEAVANHQRMLDLARQLRVEREREESLAHQKQEQKNQLFQAEQRLQRCQIQLKDLQQAAADEKPESLMKRLEEDIKFNSYMVSEKLPRELENMRKVVQYLQKVASEPAMGQAELRELEDKIRETNTEINHLIEKRMMRNDPMDDKLSLFRQQAAIIVRKKETKVEELQEAREELAAVERELNMKTSQAQERGGELIRGDEFKRYVAKMRGKSSTYKKKRQEIAELKAEYGVLQRTEEILRERHTAGQQQLQSLEAQRGISGYSDTQEELERVSAIKSELDEMKGRTLDDMSEMVKKLNSVIAEKKSALSPLIKDLRALRQEHAELAPEYEEKKAQYETCAAGLESNRSTLEQEVRTLREETAQEESRYHHINCEREIIESQIQRAAEQSKISQSMDVQVRRTALREKYIANTAEQESLGKVLRQQVKQVRENQEPNMRQMKMWKDLETLLECKKQCYLKAQSQAPIGHVIQDVGKDMLVL

Target ORF information:

RefSeq Version	NM_001002313.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio intraflagellar transport 81 homolog (ift81), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002313.1

ORF Insert Sequence:

ATGAGCGAAC AGCTTAAATT TATCGTTGAA CAGCTGAACA AGGAGCCTTT CAAGAAAAAC 
TTCAATCTCA TTACATTCGA CTCTCTGGAG CCAATGCAGC TTCTGCAGAC TTTAAGTGAT 
GTTCTGGCTG AAATTGATCC AAAGCAAGCC ATTGATATAC GTGAGGAATT GCCTGAGCAG 
ACAGCCAAGA GAATGTTCAC TCTTCTGGGA ATGCTGAAAT ACAAACCTTC TGGAGGAATG 
TCTGAAGTAA GCAGTTTTCG TCAGGGTTTG GTGTCGGGCA GTAAGCCTGT GGTGCACCCC 
ATCCTGCACT GGCTGTTACA GAGGATTCCA GAGCTGAAGA AGAGAGCGTA TCTGGCCAGA 
TTCCTCGTCA AACTAGAGGT TCCTGCTGAG TTTCTGCAAG ATGATGTCAT TGCTGAGACC 
TACCACCAGT ATGAGGAGCT GGTGGAGGGC TTCAAGAACA TCCACAAAGA GTGTGAACAG 
TTAAAGAGTT CTGGGTTCTC CACTGCTGAG ATTCGACGGG ACATTGTTGC AATGGAGGAG 
GAGAAGGATC AGCTCATCAA AAGAGTGGAA CGTCTTAGAA AAAGGGTGGA GGCAGTGGCA 
AACCACCAGC GCATGCTGGA CCTGGCCAGA CAGTTAAGAG TGGAAAGAGA AAGAGAGGAA 
TCACTCGCTC ATCAGAAACA GGAGCAGAAA AACCAGCTCT TCCAGGCAGA GCAGAGACTG 
CAGAGATGTC AGATCCAGCT CAAAGACTTA CAGCAGGCAG CAGCAGACGA AAAGCCAGAG 
AGCCTTATGA AGAGACTTGA GGAGGACATT AAGTTCAACT CCTACATGGT GTCTGAGAAG 
CTCCCCAGAG AGTTGGAGAA TATGAGAAAG GTGGTGCAGT ACCTACAGAA GGTGGCGTCT 
GAGCCTGCTA TGGGCCAGGC AGAACTGAGG GAGCTTGAGG ACAAGATAAG AGAAACCAAC 
ACTGAGATCA ATCATTTGAT TGAAAAGAGG ATGATGCGGA ACGACCCAAT GGATGATAAA 
CTCTCTCTTT TCAGGCAGCA GGCCGCTATT ATAGTCCGTA AGAAGGAAAC AAAGGTGGAG 
GAGTTGCAGG AGGCCCGTGA GGAGCTGGCG GCAGTGGAAA GAGAGCTGAA CATGAAGACC 
AGTCAGGCAC AAGAGCGAGG AGGAGAGCTG ATCCGTGGAG ATGAGTTTAA GAGATATGTG 
GCCAAGATGC GTGGTAAGAG CAGCACATAT AAGAAAAAGC GTCAGGAGAT AGCTGAGCTG 
AAGGCAGAGT ATGGTGTGCT ACAGAGAACC GAAGAGATCC TGAGAGAACG ACACACAGCT 
GGACAACAGC AGCTGCAATC TCTGGAGGCT CAGCGGGGAA TCTCAGGCTA CAGCGATACT 
CAGGAGGAGT TGGAGAGAGT TTCTGCTATC AAGAGCGAGC TGGATGAGAT GAAGGGAAGA 
ACACTGGATG ACATGTCTGA AATGGTAAAG AAGCTGAATT CAGTGATAGC AGAGAAGAAG 
TCTGCTCTAT CTCCGCTGAT AAAGGACCTG AGAGCTCTAA GACAGGAACA CGCGGAGCTC 
GCACCAGAGT ACGAGGAGAA AAAGGCTCAG TATGAAACCT GTGCTGCAGG ACTCGAGAGC 
AACAGATCCA CGCTGGAACA GGAAGTGAGA ACACTCAGGG AAGAGACGGC ACAGGAAGAA 
AGCCGATATC ATCACATTAA CTGCGAGAGA GAGATCATTG AGAGTCAAAT TCAGAGAGCG 
GCAGAACAAA GCAAGATCAG TCAGTCAATG GACGTACAGG TGCGGAGGAC GGCTCTCAGA 
GAGAAGTACA TTGCAAACAC AGCAGAGCAG GAGTCCTTGG GCAAGGTTCT CCGTCAGCAG 
GTGAAGCAGG TGCGAGAGAA CCAGGAGCCC AATATGAGAC AGATGAAGAT GTGGAAGGAT 
CTGGAAACGC TGCTGGAGTG CAAGAAACAG TGCTACCTCA AGGCTCAGAG CCAGGCGCCT 
ATTGGGCACG TCATCCAGGA TGTTGGCAAG GATATGCTGG TTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes.
Proceedings of the National Academy of Sciences of the United States of America114(6)E941-E950(2017 Feb)
Ribas L,Liew WC,D?az N,Sreenivasan R,Orb?n L,Piferrer F

Function-driven discovery of disease genes in zebrafish using an integrated genomics big data resource.
Nucleic acids research44(20)9611-9623(2016 Nov)
Shim H,Kim JH,Kim CY,Hwang S,Kim H,Yang S,Lee JE,Lee I

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish Tsc1 reveals functional interactions between the cilium and the TOR pathway.
Human molecular genetics18(4)595-606(2009 Feb)
DiBella LM,Park A,Sun Z

A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney.
Development (Cambridge, England)131(16)4085-93(2004 Aug)
Sun Z,Amsterdam A,Pazour GJ,Cole DG,Miller MS,Hopkins N

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ift81 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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