kdm5bb cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm5bb
Entrez Gene ID	415256
Full Name	lysine (K)-specific demethylase 5Bb
Synonyms	cb264,jarid1bb,plu1,wu:fc44h11,zgc:85741
General protein information	Preferred Names lysine (K)-specific demethylase 5Bb Names lysine-specific demethylase 5B-B histone demethylase JARID1B-B jumonji/ARID domain-containing protein 1B-B putative DNA/chromatin binding motif
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167024.3	XP_005167081.1	lysine-specific demethylase 5B-B isoform X1
XM_017357312.1	XP_017212801.1	lysine-specific demethylase 5B-B isoform X4
XM_005167024.4	XP_005167081.1	lysine-specific demethylase 5B-B isoform X1
XM_009303989.2	XP_009302264.1	lysine-specific demethylase 5B-B isoform X2
XM_017357311.1	XP_017212800.1	lysine-specific demethylase 5B-B isoform X3
NM_001002166.2	NP_001002166.2	lysine-specific demethylase 5B-B
XM_009303989.3	XP_009302264.1	lysine-specific demethylase 5B-B isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KDM5B	XP_002694248.2
Rattus norvegicus (Norway rat)	Kdm5b	NP_001100647.1
Canis lupus familiaris (dog)	KDM5B	XP_005622266.1
Gallus gallus (chicken)	KDM5B	NP_001026200.1
Danio rerio (zebrafish)	kdm5bb	NP_001002166.1
Xenopus tropicalis (tropical clawed frog)	kdm5b	XP_004910700.1
Mus musculus (house mouse)	Kdm5b	NP_690855.2
Caenorhabditis elegans (roundworm)	rbr-2	NP_502032.2
Pan troglodytes (chimpanzee)	KDM5B	XP_514104.3
Homo sapiens (human)	KDM5B	NP_006609.3
	LOC706437	XP_002808303.1

Gene Ontology
Bibliography

Related articles in PubMed

Enhancer activity-based identification of functional enhancers using zebrafish embryos.
Taminato T, Yokota D, Araki S, Ovara H, Yamasu K, Kawamura A
Genomics108(2)102-7(2016 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following kdm5bb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm5bb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59957	XM_017357311.1 Latest version!	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa98397	NM_001002166.2 Latest version!	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa14210	XM_005167024.4 Latest version!	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41011	XM_009303989.3 Latest version!	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41012	XM_017357312.1 Latest version!	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa14210	XM_005167024.3	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41011	XM_009303989.2	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm5bb ( XM_017357311.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212800.1 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59957
Clone ID Related Accession (Same CDS sequence)	XM_017357311.1
Accession Version	XM_017357311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4449bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 5B-B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGTCTC AGCCTAAGAG CAGTCACGTC ACGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCTGCAGC TGTGTGGCTC TCCATCTCGG 
TGTCAGGACT GGGCAGTTTC AGGGCAGGAG CAGACGGTGT TTTACACAGA ACAGCGCTGT 
ATTCCTCTCC AGGGTCTCAG TCCAGAGCTG GAGGAGCTGA TGGTGGAGGG TTTGGTCCTG 
CAGGTTTCTT TGCCTGAGAC CCAGCAGCTC TACAGGCTTC TGCTGTCTGG CCCGCCAACC 
ACAAACACGT CCCACGCAGA ACACAAGTCC TACCTCACAC CACCACAGAC AGAGACAGAT 
GCAATCACTT CAGCAGAAAA GAAAGCCAAG CGGCGAATGA ACCGAGACGA GGTGGATATC 
AGGGAAAGAG GGACGAAGCT GAAAAGCAAG AAGCAGAGGA TGATGGGTGT GGAGAAGCGG 
AGAGAGAGGA AAGCAGCGTC AGTCTCTGCG TCAGACATGT CTCAGTCAGA AGACTCAGAG 
GAGGACATGA CTCTGTGTCC TGCAGAGAGC TGTCTCCAGC CTGAGGGAGA AGAGGTGGAC 
TGGGTGCAGT GTGATTGCTG TAACCGCTGG TTTCATATGA TTTGTGTGGG AGTCTCAGCA 
GAACTGGCAG CCGAGGAGGA TTACATGTGT GTGAGCTGCA GCACAAGTCA CATGGACCGC 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212800.1
CDS	16..4464
Translation	MWSQPKSSHVTDWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWDLQGCTLKIPHVERKILDLYQLNKLVADEGGFDLVCRERRWTKIAMTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLAPEFAVKLTGFGEPPVLEECLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKLQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	XM_017357311.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357311.1

ORF Insert Sequence:

ATGTGGTCTC AGCCTAAGAG CAGTCACGTC ACGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCTGCAGC TGTGTGGCTC TCCATCTCGG 
TGTCAGGACT GGGCAGTTTC AGGGCAGGAG CAGACGGTGT TTTACACAGA ACAGCGCTGT 
ATTCCTCTCC AGGGTCTCAG TCCAGAGCTG GAGGAGCTGA TGGTGGAGGG TTTGGTCCTG 
CAGGTTTCTT TGCCTGAGAC CCAGCAGCTC TACAGGCTTC TGCTGTCTGG CCCGCCAACC 
ACAAACACGT CCCACGCAGA ACACAAGTCC TACCTCACAC CACCACAGAC AGAGACAGAT 
GCAATCACTT CAGCAGAAAA GAAAGCCAAG CGGCGAATGA ACCGAGACGA GGTGGATATC 
AGGGAAAGAG GGACGAAGCT GAAAAGCAAG AAGCAGAGGA TGATGGGTGT GGAGAAGCGG 
AGAGAGAGGA AAGCAGCGTC AGTCTCTGCG TCAGACATGT CTCAGTCAGA AGACTCAGAG 
GAGGACATGA CTCTGTGTCC TGCAGAGAGC TGTCTCCAGC CTGAGGGAGA AGAGGTGGAC 
TGGGTGCAGT GTGATTGCTG TAACCGCTGG TTTCATATGA TTTGTGTGGG AGTCTCAGCA 
GAACTGGCAG CCGAGGAGGA TTACATGTGT GTGAGCTGCA GCACAAGTCA CATGGACCGC 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5bb ( NM_001002166.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002166.2 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), mRNA.

Price & Availability↑

CloneID	ODa98397
Clone ID Related Accession (Same CDS sequence)	NM_001002166.2
Accession Version	NM_001002166.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 5B-B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH449360.1 and BX664742.15. On Dec 2, 2016 this sequence version replaced NM_001002166.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC071280.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGTGTCT GCAGAAGCCT 
GGGGATGAGG TGAATGACAT TGACAAAGAG TATAAACCCC ACGACCTACT GCAGAGGCAA 
AATGTTCCAC TTCAGCCTAG CAACACGAGC GCACCGGCTA GACGAGCCAA GAGGATGAAA 
ACAGAGTCTG GCTGTATTAA GTCAGAGGAA GGTGAGGGGG TTGAGAACAA GCCAAATCTC 
AGGAGGAGGA TGGGTTCTTT CGTTGTCAAG ACTGAACAAA AAAAGGAGAT CCCTATACAG 
GTGAAAGAGG AACCTGTGGA GATAAAAGAG CTGAATCCTG AACCGGAGAA AAGCAAACCG 
AAGAAGAAAA ACATCCCCCC TCCTCCAGTC AGCATGGTGG ACCTCTATGT GTGTTTAGTG 
TGTGGAAAGG GAAACGACGA GGATCGGTTG TTGCTATGCG ACGGTTGTGA TGACAGCTAC 
CACACATTCT GCTTAATCCC GCCCCTAACC GATGTTCCGA AAGGTGATTG GCGGTGCCCT 
AAATGTCTGA CTCAGGAGTG CTGTAAACCT CAGGAGGCAT TTGGCTTCGA GCAAGCACAC 
AGAGACTATA CTCTCAAAGC CTTTGGGGAA ATGGCAGACT CCTTCAAGTC AGATTATTTC 
AACATGCCTG TTCATATGGT ACCCACTGAA TTAGTGGAAA AAGAGTTCTG GAGGTTGGTC 
GGCACCATTC AGGAGGATGT GACAGTGGAA TATGGCGCTG ACATTGCCTC AAAAGAGTTC 
GGAAGTGGGT TTCCAATCAA AGGTGGAAGA TTTAAGATTG CACCGCACGA TGAGAAATAT 
CTTCAGTGTG GTTGGAACCT GAACAATATG GCAATGATGA CACCATCAGT GCTGACACAC 
GTCACTGCTG ATATCTGTGG AATGACTTTG CCATGGCTGT ATGTGGGCAT GTGCTTCTCA 
TCGTTCTGCT GGCACATTGA GGACCACTGG AGCTATTCCA TCAACTACCT GCACTGGGGA 
GAACCTAAGA CCTGGTATGG TGCTCCGGGT TTTGCTGCTG AGCAGTTGGA AGCCGTCATG 
AAGAAACTGG CCCCAGAACT ATTTGATTCT CAGCCCGACC TTCTACACCA GCTTGTCACA 
ATCATGAATC CTAATACACT CATGGCCCAT GGAGTACCAA TTTACAGAAC CAATCAGTGC 
GCAGGAGAGT TTGTCATTAC TTTTCCGCGA TCCTATCACA GTGGCTTCAA CCAGGGCTTC 
AACTTTGCAG AGGCTGTGAA CTTTTGCACA GTGGACTGGA TGCCACTGGG GCGTCAGTGT 
GTGGATCACT ACCGCCAGCT GCATCGCTAC TGTGTGTTTT CTCATGATGA GATGGTGTGT 
AATATGGCCA TGAAGGCTGA CTGTCTTGAT GTGGTTCTGG CGTCAGCCGT ACAGAAGGAC 
ATGCAGCTCA TGATCAAAGA AGAGAGAGAG CTGCGGGAGA AAGTCCGTAA GATGGGGGTG 
GCCCAGTGCG AGTTGTTTCA ATATGACTTG CTGGCAGATG ATGAGAGACA GTGTGTGAAG 
TGTCGCACCA CCTGTTACCT GTCTGCTCTC ACCTGTCCCT GCAGACCAGG AGTCCAGGTG 
TGTCTGTACC ACACCCACGA CCTCTGCTCC TGCCCTATCT CCAACTACAC TCTCAATTAT 
CGTTTCACAC TGGATGACCT TTACCCGATG ATGAACGCTG TGCGGCAGAG GGCAGAGTAT 
TACGATGATT GGGCCTCAAG AGTGACAGAG GTCATGGAAG CAAAGCTAGA CAAGAAACGC 
AATGTCACTG TGTTTCGCAC ACTTCTGGAG GAGTCCAATG AGCAGTCGTT CCCAGAGAAT 
GACCTGTTAC GTCAGCTGAG GCTGGTTACT CAAGATGCTG AGAAATGCTC GTCTGTTGCC 
CAGCAACTGC TAAATGGCAA GCGGCAGACC AGGTACCGCA CAGGCAAGGC TAAATCTCCT 
AATCAGCTCA CAGTGGAGGA AATGAGATCA TTCGTCCGCC AGCTTTATAA CCTCCCCTGC 
AGCCTGACAC AAGCACCGCT TCTCAAGGAG CTGCTTAACA GCATAGAGGA TTTCCAGCAG 
CACAGTGAGA AGCTTCTATC TGATGAAGTG AGCGCAGATG CAGTGAGCGA GATCGAGTCT 
TTGCTGGAGG AAGGCTCTCA GTTCGATGTG TTTCTTCCGG AGCTGCCGCT GCTACGGGAG 
CGTCTGGAAC AGGCTCGATG GCTCACCGGA GTTCACCAAG CAGAGGATCC CGTAGCAAAC 
CCCTGCGGTC TCTCTCTGGA GAGCATGCGG AGGCTGATCG ACCGAGGGGT TGGACTGACG 
CCTCATCCCT CCATAGAACG CATGATGGCT CGATTGCAGG AGCTGCTTAC CGTCTCAGAG 
GAGCTGGAGG AGAATGCGCA GGCTCTGCTG AAGGCCAGAC CTCCAGAGAG TTTAGAGACA 
CTCTGCTCCA TGTTGACTCA GGTGGAAGGT GTACCTGCAT ACCTACCAAA CTGCCTCCTA 
CTACAGGACA CAGTGAACAG GGCTAAAGAA TGGCTTCAAG AAGCAGAGAG TTTACAGGTG 
GGAGGTCAGG TGCCGGTTTT CAGCTCGCTC TCTGATATGG TTCTGAGAGC TCATTCTATT 
CCTGTGCGAC TGGAGCCCCT GGACCAGCTG GAGGTTCAGG TATCAGAGGT GCAGTCCTGG 
AAGGAGACGG CAGCTAAAAC CTTCCTAATC AAGAGCTCCC CCTTCACGCT GCTGGAGGTC 
TTATGTCCTC GATGGGAAAT GGGCAGCTCT TTGAAAAAGA AGAAGATGAG AAAGATGAAG 
GGCGACTGTG TGTCATCAGG GAAGAAGAAG TTTGTGAAGC TGGACAGTAT GAGTGACGTC 
GAGAGAGCAC TTTCAGACAG TAAAGACTCT GCATCTGCTA TGTTTACTTT GGCTGAGGTG 
CGTTTAAAAG AGCTGGAGTC ACTCTGCTCT CTGCGTGCAT CGAATGAATC CAAGCTCCTG 
CCCACAGCCG ACTGCGCCTC GCTCAAAGTG TGTGTGTGTC AGAAACCAGC CATGGGCGCC 
ATGCTGCAGT GTGAGCTGTG CCGGGACGCT TTCCACAGCG TGTGTGTGCG GGGTCCCTCA 
GACCCCCTGG ACCCTGAAGC GTGGCTTTGT CCATTATGCC TGAGGTCGAC CAAACCGCCT 
CTGGATAAGA TCAGGAGCCT GCTGTCTTCA CTGCAGCGGA TCCGCGTGCG CTTACCTGAA 
GGCGATGCTT TACGTTATAT GATCGAGCGC AGTGTGAGCT GGCAGCGGAG GGTGCGAGAG 
GTTATAGACT CTTATGGGCT CTCGTCAACC TCTCAGGATG GCAGAGGAGC TTCACCTTCA 
CAGAACCTTT ACAGGAGCAC TGGCCACAGC AGGAAGCTGC AGCTGTGTGG CTCTCCATCT 
CGGTGTCAGG ACTGGGCAGT TTCAGGGCAG GAGCAGACGG TGTTTTACAC AGAACAGCGC 
TGTATTCCTC TCCAGGGTCT CAGTCCAGAG CTGGAGGAGC TGATGGTGGA GGGTTTGGTC 
CTGCAGGTTT CTTTGCCTGA GACCCAGCAG CTCTACAGGC TTCTGCTGTC TGGCCCGCCA 
ACCACAAACA CGTCCCACGC AGAACACAAG TCCTACCTCA CACCACCACA GACAGAGACA 
GATGCAATCA CTTCAGCAGA AAAGAAAGCC AAGCGGCGAA TGAACCGAGA CGAGGTGGAT 
ATCAGGGAAA GAGGGACGAA GCTGAAAAGC AAGAAGCAGA GGATGATGGG TGTGGAGAAG 
CGGAGAGAGA GGAAAGCAGC GTCAGTCTCT GCGTCAGACA TGTCTCAGTC AGAAGACTCA 
GAGGAGGACA TGACTCTGTG TCCTGCAGAG AGCTGTCTCC AGCCTGAGGG AGAAGAGGTG 
GACTGGGTGC AGTGTGATTG CTGTAACCGC TGGTTTCATA TGATTTGTGT GGGAGTCTCA 
GCAGAACTGG CAGCCGAGGA GGATTACATG TGTGTGAGCT GCAGCACAAG TCACATGGAC 
CGCCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002166.2
CDS	175..4686
Translation	MTQQGPAEFTPPPECPVFEPSWEEFKDPFAFINKIRPIAEKTGICKVRPPPDWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWDLQGCTLKIPHVERKILDLYQLNKLVADEGGFDLVCRERRWTKIAMTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLCLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKLQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	NM_001002166.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002166.2

ORF Insert Sequence:

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGTGTCT GCAGAAGCCT 
GGGGATGAGG TGAATGACAT TGACAAAGAG TATAAACCCC ACGACCTACT GCAGAGGCAA 
AATGTTCCAC TTCAGCCTAG CAACACGAGC GCACCGGCTA GACGAGCCAA GAGGATGAAA 
ACAGAGTCTG GCTGTATTAA GTCAGAGGAA GGTGAGGGGG TTGAGAACAA GCCAAATCTC 
AGGAGGAGGA TGGGTTCTTT CGTTGTCAAG ACTGAACAAA AAAAGGAGAT CCCTATACAG 
GTGAAAGAGG AACCTGTGGA GATAAAAGAG CTGAATCCTG AACCGGAGAA AAGCAAACCG 
AAGAAGAAAA ACATCCCCCC TCCTCCAGTC AGCATGGTGG ACCTCTATGT GTGTTTAGTG 
TGTGGAAAGG GAAACGACGA GGATCGGTTG TTGCTATGCG ACGGTTGTGA TGACAGCTAC 
CACACATTCT GCTTAATCCC GCCCCTAACC GATGTTCCGA AAGGTGATTG GCGGTGCCCT 
AAATGTCTGA CTCAGGAGTG CTGTAAACCT CAGGAGGCAT TTGGCTTCGA GCAAGCACAC 
AGAGACTATA CTCTCAAAGC CTTTGGGGAA ATGGCAGACT CCTTCAAGTC AGATTATTTC 
AACATGCCTG TTCATATGGT ACCCACTGAA TTAGTGGAAA AAGAGTTCTG GAGGTTGGTC 
GGCACCATTC AGGAGGATGT GACAGTGGAA TATGGCGCTG ACATTGCCTC AAAAGAGTTC 
GGAAGTGGGT TTCCAATCAA AGGTGGAAGA TTTAAGATTG CACCGCACGA TGAGAAATAT 
CTTCAGTGTG GTTGGAACCT GAACAATATG GCAATGATGA CACCATCAGT GCTGACACAC 
GTCACTGCTG ATATCTGTGG AATGACTTTG CCATGGCTGT ATGTGGGCAT GTGCTTCTCA 
TCGTTCTGCT GGCACATTGA GGACCACTGG AGCTATTCCA TCAACTACCT GCACTGGGGA 
GAACCTAAGA CCTGGTATGG TGCTCCGGGT TTTGCTGCTG AGCAGTTGGA AGCCGTCATG 
AAGAAACTGG CCCCAGAACT ATTTGATTCT CAGCCCGACC TTCTACACCA GCTTGTCACA 
ATCATGAATC CTAATACACT CATGGCCCAT GGAGTACCAA TTTACAGAAC CAATCAGTGC 
GCAGGAGAGT TTGTCATTAC TTTTCCGCGA TCCTATCACA GTGGCTTCAA CCAGGGCTTC 
AACTTTGCAG AGGCTGTGAA CTTTTGCACA GTGGACTGGA TGCCACTGGG GCGTCAGTGT 
GTGGATCACT ACCGCCAGCT GCATCGCTAC TGTGTGTTTT CTCATGATGA GATGGTGTGT 
AATATGGCCA TGAAGGCTGA CTGTCTTGAT GTGGTTCTGG CGTCAGCCGT ACAGAAGGAC 
ATGCAGCTCA TGATCAAAGA AGAGAGAGAG CTGCGGGAGA AAGTCCGTAA GATGGGGGTG 
GCCCAGTGCG AGTTGTTTCA ATATGACTTG CTGGCAGATG ATGAGAGACA GTGTGTGAAG 
TGTCGCACCA CCTGTTACCT GTCTGCTCTC ACCTGTCCCT GCAGACCAGG AGTCCAGGTG 
TGTCTGTACC ACACCCACGA CCTCTGCTCC TGCCCTATCT CCAACTACAC TCTCAATTAT 
CGTTTCACAC TGGATGACCT TTACCCGATG ATGAACGCTG TGCGGCAGAG GGCAGAGTAT 
TACGATGATT GGGCCTCAAG AGTGACAGAG GTCATGGAAG CAAAGCTAGA CAAGAAACGC 
AATGTCACTG TGTTTCGCAC ACTTCTGGAG GAGTCCAATG AGCAGTCGTT CCCAGAGAAT 
GACCTGTTAC GTCAGCTGAG GCTGGTTACT CAAGATGCTG AGAAATGCTC GTCTGTTGCC 
CAGCAACTGC TAAATGGCAA GCGGCAGACC AGGTACCGCA CAGGCAAGGC TAAATCTCCT 
AATCAGCTCA CAGTGGAGGA AATGAGATCA TTCGTCCGCC AGCTTTATAA CCTCCCCTGC 
AGCCTGACAC AAGCACCGCT TCTCAAGGAG CTGCTTAACA GCATAGAGGA TTTCCAGCAG 
CACAGTGAGA AGCTTCTATC TGATGAAGTG AGCGCAGATG CAGTGAGCGA GATCGAGTCT 
TTGCTGGAGG AAGGCTCTCA GTTCGATGTG TTTCTTCCGG AGCTGCCGCT GCTACGGGAG 
CGTCTGGAAC AGGCTCGATG GCTCACCGGA GTTCACCAAG CAGAGGATCC CGTAGCAAAC 
CCCTGCGGTC TCTCTCTGGA GAGCATGCGG AGGCTGATCG ACCGAGGGGT TGGACTGACG 
CCTCATCCCT CCATAGAACG CATGATGGCT CGATTGCAGG AGCTGCTTAC CGTCTCAGAG 
GAGCTGGAGG AGAATGCGCA GGCTCTGCTG AAGGCCAGAC CTCCAGAGAG TTTAGAGACA 
CTCTGCTCCA TGTTGACTCA GGTGGAAGGT GTACCTGCAT ACCTACCAAA CTGCCTCCTA 
CTACAGGACA CAGTGAACAG GGCTAAAGAA TGGCTTCAAG AAGCAGAGAG TTTACAGGTG 
GGAGGTCAGG TGCCGGTTTT CAGCTCGCTC TCTGATATGG TTCTGAGAGC TCATTCTATT 
CCTGTGCGAC TGGAGCCCCT GGACCAGCTG GAGGTTCAGG TATCAGAGGT GCAGTCCTGG 
AAGGAGACGG CAGCTAAAAC CTTCCTAATC AAGAGCTCCC CCTTCACGCT GCTGGAGGTC 
TTATGTCCTC GATGGGAAAT GGGCAGCTCT TTGAAAAAGA AGAAGATGAG AAAGATGAAG 
GGCGACTGTG TGTCATCAGG GAAGAAGAAG TTTGTGAAGC TGGACAGTAT GAGTGACGTC 
GAGAGAGCAC TTTCAGACAG TAAAGACTCT GCATCTGCTA TGTTTACTTT GGCTGAGGTG 
CGTTTAAAAG AGCTGGAGTC ACTCTGCTCT CTGCGTGCAT CGAATGAATC CAAGCTCCTG 
CCCACAGCCG ACTGCGCCTC GCTCAAAGTG TGTGTGTGTC AGAAACCAGC CATGGGCGCC 
ATGCTGCAGT GTGAGCTGTG CCGGGACGCT TTCCACAGCG TGTGTGTGCG GGGTCCCTCA 
GACCCCCTGG ACCCTGAAGC GTGGCTTTGT CCATTATGCC TGAGGTCGAC CAAACCGCCT 
CTGGATAAGA TCAGGAGCCT GCTGTCTTCA CTGCAGCGGA TCCGCGTGCG CTTACCTGAA 
GGCGATGCTT TACGTTATAT GATCGAGCGC AGTGTGAGCT GGCAGCGGAG GGTGCGAGAG 
GTTATAGACT CTTATGGGCT CTCGTCAACC TCTCAGGATG GCAGAGGAGC TTCACCTTCA 
CAGAACCTTT ACAGGAGCAC TGGCCACAGC AGGAAGCTGC AGCTGTGTGG CTCTCCATCT 
CGGTGTCAGG ACTGGGCAGT TTCAGGGCAG GAGCAGACGG TGTTTTACAC AGAACAGCGC 
TGTATTCCTC TCCAGGGTCT CAGTCCAGAG CTGGAGGAGC TGATGGTGGA GGGTTTGGTC 
CTGCAGGTTT CTTTGCCTGA GACCCAGCAG CTCTACAGGC TTCTGCTGTC TGGCCCGCCA 
ACCACAAACA CGTCCCACGC AGAACACAAG TCCTACCTCA CACCACCACA GACAGAGACA 
GATGCAATCA CTTCAGCAGA AAAGAAAGCC AAGCGGCGAA TGAACCGAGA CGAGGTGGAT 
ATCAGGGAAA GAGGGACGAA GCTGAAAAGC AAGAAGCAGA GGATGATGGG TGTGGAGAAG 
CGGAGAGAGA GGAAAGCAGC GTCAGTCTCT GCGTCAGACA TGTCTCAGTC AGAAGACTCA 
GAGGAGGACA TGACTCTGTG TCCTGCAGAG AGCTGTCTCC AGCCTGAGGG AGAAGAGGTG 
GACTGGGTGC AGTGTGATTG CTGTAACCGC TGGTTTCATA TGATTTGTGT GGGAGTCTCA 
GCAGAACTGG CAGCCGAGGA GGATTACATG TGTGTGAGCT GCAGCACAAG TCACATGGAC 
CGCCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5bb ( XM_005167024.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167081.1 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X1, mRNA.

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CloneID	ODa14210
Clone ID Related Accession (Same CDS sequence)	XM_005167024.3 , XM_005167024.4
Accession Version	XM_005167024.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 5B-B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167024.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCTGCAGC TGTGTGGCTC TCCATCTCGG 
TGTCAGGACT GGGCAGTTTC AGGGCAGGAG CAGACGGTGT TTTACACAGA ACAGCGCTGT 
ATTCCTCTCC AGGGTCTCAG TCCAGAGCTG GAGGAGCTGA TGGTGGAGGG TTTGGTCCTG 
CAGGTTTCTT TGCCTGAGAC CCAGCAGCTC TACAGGCTTC TGCTGTCTGG CCCGCCAACC 
ACAAACACGT CCCACGCAGA ACACAAGTCC TACCTCACAC CACCACAGAC AGAGACAGAT 
GCAATCACTT CAGCAGAAAA GAAAGCCAAG CGGCGAATGA ACCGAGACGA GGTGGATATC 
AGGGAAAGAG GGACGAAGCT GAAAAGCAAG AAGCAGAGGA TGATGGGTGT GGAGAAGCGG 
AGAGAGAGGA AAGCAGCGTC AGTCTCTGCG TCAGACATGT CTCAGTCAGA AGACTCAGAG 
GAGGACATGA CTCTGTGTCC TGCAGAGAGC TGTCTCCAGC CTGAGGGAGA AGAGGTGGAC 
TGGGTGCAGT GTGATTGCTG TAACCGCTGG TTTCATATGA TTTGTGTGGG AGTCTCAGCA 
GAACTGGCAG CCGAGGAGGA TTACATGTGT GTGAGCTGCA GCACAAGTCA CATGGACCGC 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167081.1
CDS	169..4737
Translation	MTQQGPAEFTPPPECPVFEPSWEEFKDPFAFINKIRPIAEKTGICKVRPPPDWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWDLQGCTLKIPHVERKILDLYQLNKLVADEGGFDLVCRERRWTKIAMTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLAPEFAVKLTGFGEPPVLEECLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKLQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	XM_005167024.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167024.3

ORF Insert Sequence:

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCTGCAGC TGTGTGGCTC TCCATCTCGG 
TGTCAGGACT GGGCAGTTTC AGGGCAGGAG CAGACGGTGT TTTACACAGA ACAGCGCTGT 
ATTCCTCTCC AGGGTCTCAG TCCAGAGCTG GAGGAGCTGA TGGTGGAGGG TTTGGTCCTG 
CAGGTTTCTT TGCCTGAGAC CCAGCAGCTC TACAGGCTTC TGCTGTCTGG CCCGCCAACC 
ACAAACACGT CCCACGCAGA ACACAAGTCC TACCTCACAC CACCACAGAC AGAGACAGAT 
GCAATCACTT CAGCAGAAAA GAAAGCCAAG CGGCGAATGA ACCGAGACGA GGTGGATATC 
AGGGAAAGAG GGACGAAGCT GAAAAGCAAG AAGCAGAGGA TGATGGGTGT GGAGAAGCGG 
AGAGAGAGGA AAGCAGCGTC AGTCTCTGCG TCAGACATGT CTCAGTCAGA AGACTCAGAG 
GAGGACATGA CTCTGTGTCC TGCAGAGAGC TGTCTCCAGC CTGAGGGAGA AGAGGTGGAC 
TGGGTGCAGT GTGATTGCTG TAACCGCTGG TTTCATATGA TTTGTGTGGG AGTCTCAGCA 
GAACTGGCAG CCGAGGAGGA TTACATGTGT GTGAGCTGCA GCACAAGTCA CATGGACCGC 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5bb ( XM_005167024.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167081.1 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X1, mRNA.

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CloneID	ODa14210
Clone ID Related Accession (Same CDS sequence)	XM_005167024.3 , XM_005167024.4
Accession Version	XM_005167024.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 5B-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167024.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCTGCAGC TGTGTGGCTC TCCATCTCGG 
TGTCAGGACT GGGCAGTTTC AGGGCAGGAG CAGACGGTGT TTTACACAGA ACAGCGCTGT 
ATTCCTCTCC AGGGTCTCAG TCCAGAGCTG GAGGAGCTGA TGGTGGAGGG TTTGGTCCTG 
CAGGTTTCTT TGCCTGAGAC CCAGCAGCTC TACAGGCTTC TGCTGTCTGG CCCGCCAACC 
ACAAACACGT CCCACGCAGA ACACAAGTCC TACCTCACAC CACCACAGAC AGAGACAGAT 
GCAATCACTT CAGCAGAAAA GAAAGCCAAG CGGCGAATGA ACCGAGACGA GGTGGATATC 
AGGGAAAGAG GGACGAAGCT GAAAAGCAAG AAGCAGAGGA TGATGGGTGT GGAGAAGCGG 
AGAGAGAGGA AAGCAGCGTC AGTCTCTGCG TCAGACATGT CTCAGTCAGA AGACTCAGAG 
GAGGACATGA CTCTGTGTCC TGCAGAGAGC TGTCTCCAGC CTGAGGGAGA AGAGGTGGAC 
TGGGTGCAGT GTGATTGCTG TAACCGCTGG TTTCATATGA TTTGTGTGGG AGTCTCAGCA 
GAACTGGCAG CCGAGGAGGA TTACATGTGT GTGAGCTGCA GCACAAGTCA CATGGACCGC 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167081.1
CDS	796..5364
Translation	MTQQGPAEFTPPPECPVFEPSWEEFKDPFAFINKIRPIAEKTGICKVRPPPDWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWDLQGCTLKIPHVERKILDLYQLNKLVADEGGFDLVCRERRWTKIAMTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLAPEFAVKLTGFGEPPVLEECLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKLQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	XM_005167024.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167024.4

ORF Insert Sequence:

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCTGCAGC TGTGTGGCTC TCCATCTCGG 
TGTCAGGACT GGGCAGTTTC AGGGCAGGAG CAGACGGTGT TTTACACAGA ACAGCGCTGT 
ATTCCTCTCC AGGGTCTCAG TCCAGAGCTG GAGGAGCTGA TGGTGGAGGG TTTGGTCCTG 
CAGGTTTCTT TGCCTGAGAC CCAGCAGCTC TACAGGCTTC TGCTGTCTGG CCCGCCAACC 
ACAAACACGT CCCACGCAGA ACACAAGTCC TACCTCACAC CACCACAGAC AGAGACAGAT 
GCAATCACTT CAGCAGAAAA GAAAGCCAAG CGGCGAATGA ACCGAGACGA GGTGGATATC 
AGGGAAAGAG GGACGAAGCT GAAAAGCAAG AAGCAGAGGA TGATGGGTGT GGAGAAGCGG 
AGAGAGAGGA AAGCAGCGTC AGTCTCTGCG TCAGACATGT CTCAGTCAGA AGACTCAGAG 
GAGGACATGA CTCTGTGTCC TGCAGAGAGC TGTCTCCAGC CTGAGGGAGA AGAGGTGGAC 
TGGGTGCAGT GTGATTGCTG TAACCGCTGG TTTCATATGA TTTGTGTGGG AGTCTCAGCA 
GAACTGGCAG CCGAGGAGGA TTACATGTGT GTGAGCTGCA GCACAAGTCA CATGGACCGC 
CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5bb ( XM_009303989.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302264.1 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X2, mRNA.

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CloneID	ODa41011
Clone ID Related Accession (Same CDS sequence)	XM_009303989.2 , XM_009303989.3
Accession Version	XM_009303989.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 5B-B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303989.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCAGCTGT GTGGCTCTCC ATCTCGGTGT 
CAGGACTGGG CAGTTTCAGG GCAGGAGCAG ACGGTGTTTT ACACAGAACA GCGCTGTATT 
CCTCTCCAGG GTCTCAGTCC AGAGCTGGAG GAGCTGATGG TGGAGGGTTT GGTCCTGCAG 
GTTTCTTTGC CTGAGACCCA GCAGCTCTAC AGGCTTCTGC TGTCTGGCCC GCCAACCACA 
AACACGTCCC ACGCAGAACA CAAGTCCTAC CTCACACCAC CACAGACAGA GACAGATGCA 
ATCACTTCAG CAGAAAAGAA AGCCAAGCGG CGAATGAACC GAGACGAGGT GGATATCAGG 
GAAAGAGGGA CGAAGCTGAA AAGCAAGAAG CAGAGGATGA TGGGTGTGGA GAAGCGGAGA 
GAGAGGAAAG CAGCGTCAGT CTCTGCGTCA GACATGTCTC AGTCAGAAGA CTCAGAGGAG 
GACATGACTC TGTGTCCTGC AGAGAGCTGT CTCCAGCCTG AGGGAGAAGA GGTGGACTGG 
GTGCAGTGTG ATTGCTGTAA CCGCTGGTTT CATATGATTT GTGTGGGAGT CTCAGCAGAA 
CTGGCAGCCG AGGAGGATTA CATGTGTGTG AGCTGCAGCA CAAGTCACAT GGACCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302264.1
CDS	169..4734
Translation	MTQQGPAEFTPPPECPVFEPSWEEFKDPFAFINKIRPIAEKTGICKVRPPPDWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWDLQGCTLKIPHVERKILDLYQLNKLVADEGGFDLVCRERRWTKIAMTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLAPEFAVKLTGFGEPPVLEECLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	XM_009303989.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303989.2

ORF Insert Sequence:

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCAGCTGT GTGGCTCTCC ATCTCGGTGT 
CAGGACTGGG CAGTTTCAGG GCAGGAGCAG ACGGTGTTTT ACACAGAACA GCGCTGTATT 
CCTCTCCAGG GTCTCAGTCC AGAGCTGGAG GAGCTGATGG TGGAGGGTTT GGTCCTGCAG 
GTTTCTTTGC CTGAGACCCA GCAGCTCTAC AGGCTTCTGC TGTCTGGCCC GCCAACCACA 
AACACGTCCC ACGCAGAACA CAAGTCCTAC CTCACACCAC CACAGACAGA GACAGATGCA 
ATCACTTCAG CAGAAAAGAA AGCCAAGCGG CGAATGAACC GAGACGAGGT GGATATCAGG 
GAAAGAGGGA CGAAGCTGAA AAGCAAGAAG CAGAGGATGA TGGGTGTGGA GAAGCGGAGA 
GAGAGGAAAG CAGCGTCAGT CTCTGCGTCA GACATGTCTC AGTCAGAAGA CTCAGAGGAG 
GACATGACTC TGTGTCCTGC AGAGAGCTGT CTCCAGCCTG AGGGAGAAGA GGTGGACTGG 
GTGCAGTGTG ATTGCTGTAA CCGCTGGTTT CATATGATTT GTGTGGGAGT CTCAGCAGAA 
CTGGCAGCCG AGGAGGATTA CATGTGTGTG AGCTGCAGCA CAAGTCACAT GGACCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5bb ( XM_009303989.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302264.1 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X2, mRNA.

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CloneID	ODa41011
Clone ID Related Accession (Same CDS sequence)	XM_009303989.2 , XM_009303989.3
Accession Version	XM_009303989.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 5B-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303989.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCAGCTGT GTGGCTCTCC ATCTCGGTGT 
CAGGACTGGG CAGTTTCAGG GCAGGAGCAG ACGGTGTTTT ACACAGAACA GCGCTGTATT 
CCTCTCCAGG GTCTCAGTCC AGAGCTGGAG GAGCTGATGG TGGAGGGTTT GGTCCTGCAG 
GTTTCTTTGC CTGAGACCCA GCAGCTCTAC AGGCTTCTGC TGTCTGGCCC GCCAACCACA 
AACACGTCCC ACGCAGAACA CAAGTCCTAC CTCACACCAC CACAGACAGA GACAGATGCA 
ATCACTTCAG CAGAAAAGAA AGCCAAGCGG CGAATGAACC GAGACGAGGT GGATATCAGG 
GAAAGAGGGA CGAAGCTGAA AAGCAAGAAG CAGAGGATGA TGGGTGTGGA GAAGCGGAGA 
GAGAGGAAAG CAGCGTCAGT CTCTGCGTCA GACATGTCTC AGTCAGAAGA CTCAGAGGAG 
GACATGACTC TGTGTCCTGC AGAGAGCTGT CTCCAGCCTG AGGGAGAAGA GGTGGACTGG 
GTGCAGTGTG ATTGCTGTAA CCGCTGGTTT CATATGATTT GTGTGGGAGT CTCAGCAGAA 
CTGGCAGCCG AGGAGGATTA CATGTGTGTG AGCTGCAGCA CAAGTCACAT GGACCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302264.1
CDS	796..5361
Translation	MTQQGPAEFTPPPECPVFEPSWEEFKDPFAFINKIRPIAEKTGICKVRPPPDWQPPFACDVDRLHFTPRIQRLNELEAQTRVKLNFLDQIAKFWDLQGCTLKIPHVERKILDLYQLNKLVADEGGFDLVCRERRWTKIAMTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLAPEFAVKLTGFGEPPVLEECLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	XM_009303989.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303989.3

ORF Insert Sequence:

ATGACCCAGC AGGGCCCGGC CGAGTTTACC CCACCGCCGG AGTGCCCGGT GTTCGAGCCC 
AGTTGGGAGG AATTTAAAGA CCCGTTTGCG TTTATCAATA AAATCCGACC TATCGCCGAG 
AAAACCGGGA TCTGCAAGGT TCGACCGCCT CCGGACTGGC AGCCCCCATT CGCATGTGAC 
GTCGACAGAC TTCATTTTAC TCCACGGATC CAGAGGCTAA ATGAGCTAGA GGCTCAAACC 
AGAGTCAAAC TCAACTTCTT GGATCAGATC GCCAAATTTT GGGATCTACA AGGATGCACG 
CTCAAAATTC CCCACGTGGA GAGGAAAATC CTCGATTTGT ACCAGCTCAA CAAATTGGTT 
GCAGATGAGG GTGGGTTCGA TTTAGTTTGT AGAGAGAGGA GATGGACCAA GATCGCCATG 
ACGATGGGTT TTGCCCCAGG CAAAGCTGTG GGCTCCCACC TGAGAGCCCA TTACGAGAGG 
ATCCTGTACC CGTACAACCT CTTTCAGTCT GGGACAAATC TGCTGGCTCC TGAATTTGCA 
GTCAAACTGA CAGGTTTTGG GGAGCCTCCT GTGCTGGAGG AGTGTCTGCA GAAGCCTGGG 
GATGAGGTGA ATGACATTGA CAAAGAGTAT AAACCCCACG ACCTACTGCA GAGGCAAAAT 
GTTCCACTTC AGCCTAGCAA CACGAGCGCA CCGGCTAGAC GAGCCAAGAG GATGAAAACA 
GAGTCTGGCT GTATTAAGTC AGAGGAAGGT GAGGGGGTTG AGAACAAGCC AAATCTCAGG 
AGGAGGATGG GTTCTTTCGT TGTCAAGACT GAACAAAAAA AGGAGATCCC TATACAGGTG 
AAAGAGGAAC CTGTGGAGAT AAAAGAGCTG AATCCTGAAC CGGAGAAAAG CAAACCGAAG 
AAGAAAAACA TCCCCCCTCC TCCAGTCAGC ATGGTGGACC TCTATGTGTG TTTAGTGTGT 
GGAAAGGGAA ACGACGAGGA TCGGTTGTTG CTATGCGACG GTTGTGATGA CAGCTACCAC 
ACATTCTGCT TAATCCCGCC CCTAACCGAT GTTCCGAAAG GTGATTGGCG GTGCCCTAAA 
TGTCTGACTC AGGAGTGCTG TAAACCTCAG GAGGCATTTG GCTTCGAGCA AGCACACAGA 
GACTATACTC TCAAAGCCTT TGGGGAAATG GCAGACTCCT TCAAGTCAGA TTATTTCAAC 
ATGCCTGTTC ATATGGTACC CACTGAATTA GTGGAAAAAG AGTTCTGGAG GTTGGTCGGC 
ACCATTCAGG AGGATGTGAC AGTGGAATAT GGCGCTGACA TTGCCTCAAA AGAGTTCGGA 
AGTGGGTTTC CAATCAAAGG TGGAAGATTT AAGATTGCAC CGCACGATGA GAAATATCTT 
CAGTGTGGTT GGAACCTGAA CAATATGGCA ATGATGACAC CATCAGTGCT GACACACGTC 
ACTGCTGATA TCTGTGGAAT GACTTTGCCA TGGCTGTATG TGGGCATGTG CTTCTCATCG 
TTCTGCTGGC ACATTGAGGA CCACTGGAGC TATTCCATCA ACTACCTGCA CTGGGGAGAA 
CCTAAGACCT GGTATGGTGC TCCGGGTTTT GCTGCTGAGC AGTTGGAAGC CGTCATGAAG 
AAACTGGCCC CAGAACTATT TGATTCTCAG CCCGACCTTC TACACCAGCT TGTCACAATC 
ATGAATCCTA ATACACTCAT GGCCCATGGA GTACCAATTT ACAGAACCAA TCAGTGCGCA 
GGAGAGTTTG TCATTACTTT TCCGCGATCC TATCACAGTG GCTTCAACCA GGGCTTCAAC 
TTTGCAGAGG CTGTGAACTT TTGCACAGTG GACTGGATGC CACTGGGGCG TCAGTGTGTG 
GATCACTACC GCCAGCTGCA TCGCTACTGT GTGTTTTCTC ATGATGAGAT GGTGTGTAAT 
ATGGCCATGA AGGCTGACTG TCTTGATGTG GTTCTGGCGT CAGCCGTACA GAAGGACATG 
CAGCTCATGA TCAAAGAAGA GAGAGAGCTG CGGGAGAAAG TCCGTAAGAT GGGGGTGGCC 
CAGTGCGAGT TGTTTCAATA TGACTTGCTG GCAGATGATG AGAGACAGTG TGTGAAGTGT 
CGCACCACCT GTTACCTGTC TGCTCTCACC TGTCCCTGCA GACCAGGAGT CCAGGTGTGT 
CTGTACCACA CCCACGACCT CTGCTCCTGC CCTATCTCCA ACTACACTCT CAATTATCGT 
TTCACACTGG ATGACCTTTA CCCGATGATG AACGCTGTGC GGCAGAGGGC AGAGTATTAC 
GATGATTGGG CCTCAAGAGT GACAGAGGTC ATGGAAGCAA AGCTAGACAA GAAACGCAAT 
GTCACTGTGT TTCGCACACT TCTGGAGGAG TCCAATGAGC AGTCGTTCCC AGAGAATGAC 
CTGTTACGTC AGCTGAGGCT GGTTACTCAA GATGCTGAGA AATGCTCGTC TGTTGCCCAG 
CAACTGCTAA ATGGCAAGCG GCAGACCAGG TACCGCACAG GCAAGGCTAA ATCTCCTAAT 
CAGCTCACAG TGGAGGAAAT GAGATCATTC GTCCGCCAGC TTTATAACCT CCCCTGCAGC 
CTGACACAAG CACCGCTTCT CAAGGAGCTG CTTAACAGCA TAGAGGATTT CCAGCAGCAC 
AGTGAGAAGC TTCTATCTGA TGAAGTGAGC GCAGATGCAG TGAGCGAGAT CGAGTCTTTG 
CTGGAGGAAG GCTCTCAGTT CGATGTGTTT CTTCCGGAGC TGCCGCTGCT ACGGGAGCGT 
CTGGAACAGG CTCGATGGCT CACCGGAGTT CACCAAGCAG AGGATCCCGT AGCAAACCCC 
TGCGGTCTCT CTCTGGAGAG CATGCGGAGG CTGATCGACC GAGGGGTTGG ACTGACGCCT 
CATCCCTCCA TAGAACGCAT GATGGCTCGA TTGCAGGAGC TGCTTACCGT CTCAGAGGAG 
CTGGAGGAGA ATGCGCAGGC TCTGCTGAAG GCCAGACCTC CAGAGAGTTT AGAGACACTC 
TGCTCCATGT TGACTCAGGT GGAAGGTGTA CCTGCATACC TACCAAACTG CCTCCTACTA 
CAGGACACAG TGAACAGGGC TAAAGAATGG CTTCAAGAAG CAGAGAGTTT ACAGGTGGGA 
GGTCAGGTGC CGGTTTTCAG CTCGCTCTCT GATATGGTTC TGAGAGCTCA TTCTATTCCT 
GTGCGACTGG AGCCCCTGGA CCAGCTGGAG GTTCAGGTAT CAGAGGTGCA GTCCTGGAAG 
GAGACGGCAG CTAAAACCTT CCTAATCAAG AGCTCCCCCT TCACGCTGCT GGAGGTCTTA 
TGTCCTCGAT GGGAAATGGG CAGCTCTTTG AAAAAGAAGA AGATGAGAAA GATGAAGGGC 
GACTGTGTGT CATCAGGGAA GAAGAAGTTT GTGAAGCTGG ACAGTATGAG TGACGTCGAG 
AGAGCACTTT CAGACAGTAA AGACTCTGCA TCTGCTATGT TTACTTTGGC TGAGGTGCGT 
TTAAAAGAGC TGGAGTCACT CTGCTCTCTG CGTGCATCGA ATGAATCCAA GCTCCTGCCC 
ACAGCCGACT GCGCCTCGCT CAAAGTGTGT GTGTGTCAGA AACCAGCCAT GGGCGCCATG 
CTGCAGTGTG AGCTGTGCCG GGACGCTTTC CACAGCGTGT GTGTGCGGGG TCCCTCAGAC 
CCCCTGGACC CTGAAGCGTG GCTTTGTCCA TTATGCCTGA GGTCGACCAA ACCGCCTCTG 
GATAAGATCA GGAGCCTGCT GTCTTCACTG CAGCGGATCC GCGTGCGCTT ACCTGAAGGC 
GATGCTTTAC GTTATATGAT CGAGCGCAGT GTGAGCTGGC AGCGGAGGGT GCGAGAGGTT 
ATAGACTCTT ATGGGCTCTC GTCAACCTCT CAGGATGGCA GAGGAGCTTC ACCTTCACAG 
AACCTTTACA GGAGCACTGG CCACAGCAGG AAGCAGCTGT GTGGCTCTCC ATCTCGGTGT 
CAGGACTGGG CAGTTTCAGG GCAGGAGCAG ACGGTGTTTT ACACAGAACA GCGCTGTATT 
CCTCTCCAGG GTCTCAGTCC AGAGCTGGAG GAGCTGATGG TGGAGGGTTT GGTCCTGCAG 
GTTTCTTTGC CTGAGACCCA GCAGCTCTAC AGGCTTCTGC TGTCTGGCCC GCCAACCACA 
AACACGTCCC ACGCAGAACA CAAGTCCTAC CTCACACCAC CACAGACAGA GACAGATGCA 
ATCACTTCAG CAGAAAAGAA AGCCAAGCGG CGAATGAACC GAGACGAGGT GGATATCAGG 
GAAAGAGGGA CGAAGCTGAA AAGCAAGAAG CAGAGGATGA TGGGTGTGGA GAAGCGGAGA 
GAGAGGAAAG CAGCGTCAGT CTCTGCGTCA GACATGTCTC AGTCAGAAGA CTCAGAGGAG 
GACATGACTC TGTGTCCTGC AGAGAGCTGT CTCCAGCCTG AGGGAGAAGA GGTGGACTGG 
GTGCAGTGTG ATTGCTGTAA CCGCTGGTTT CATATGATTT GTGTGGGAGT CTCAGCAGAA 
CTGGCAGCCG AGGAGGATTA CATGTGTGTG AGCTGCAGCA CAAGTCACAT GGACCGCCGG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm5bb ( XM_017357312.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212801.1 Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa41012
Clone ID Related Accession (Same CDS sequence)	XM_017357312.1
Accession Version	XM_017357312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 5B-B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGATGG GTTTTGCCCC AGGCAAAGCT GTGGGCTCCC ACCTGAGAGC CCATTACGAG 
AGGATCCTGT ACCCGTACAA CCTCTTTCAG TCTGGGACAA ATCTGCTGGC TCCTGAATTT 
GCAGTCAAAC TGACAGGTTT TGGGGAGCCT CCTGTGCTGG AGGAGTGTCT GCAGAAGCCT 
GGGGATGAGG TGAATGACAT TGACAAAGAG TATAAACCCC ACGACCTACT GCAGAGGCAA 
AATGTTCCAC TTCAGCCTAG CAACACGAGC GCACCGGCTA GACGAGCCAA GAGGATGAAA 
ACAGAGTCTG GCTGTATTAA GTCAGAGGAA GGTGAGGGGG TTGAGAACAA GCCAAATCTC 
AGGAGGAGGA TGGGTTCTTT CGTTGTCAAG ACTGAACAAA AAAAGGAGAT CCCTATACAG 
GTGAAAGAGG AACCTGTGGA GATAAAAGAG CTGAATCCTG AACCGGAGAA AAGCAAACCG 
AAGAAGAAAA ACATCCCCCC TCCTCCAGTC AGCATGGTGG ACCTCTATGT GTGTTTAGTG 
TGTGGAAAGG GAAACGACGA GGATCGGTTG TTGCTATGCG ACGGTTGTGA TGACAGCTAC 
CACACATTCT GCTTAATCCC GCCCCTAACC GATGTTCCGA AAGGTGATTG GCGGTGCCCT 
AAATGTCTGA CTCAGGAGTG CTGTAAACCT CAGGAGGCAT TTGGCTTCGA GCAAGCACAC 
AGAGACTATA CTCTCAAAGC CTTTGGGGAA ATGGCAGACT CCTTCAAGTC AGATTATTTC 
AACATGCCTG TTCATATGGT ACCCACTGAA TTAGTGGAAA AAGAGTTCTG GAGGTTGGTC 
GGCACCATTC AGGAGGATGT GACAGTGGAA TATGGCGCTG ACATTGCCTC AAAAGAGTTC 
GGAAGTGGGT TTCCAATCAA AGGTGGAAGA TTTAAGATTG CACCGCACGA TGAGAAATAT 
CTTCAGTGTG GTTGGAACCT GAACAATATG GCAATGATGA CACCATCAGT GCTGACACAC 
GTCACTGCTG ATATCTGTGG AATGACTTTG CCATGGCTGT ATGTGGGCAT GTGCTTCTCA 
TCGTTCTGCT GGCACATTGA GGACCACTGG AGCTATTCCA TCAACTACCT GCACTGGGGA 
GAACCTAAGA CCTGGTATGG TGCTCCGGGT TTTGCTGCTG AGCAGTTGGA AGCCGTCATG 
AAGAAACTGG CCCCAGAACT ATTTGATTCT CAGCCCGACC TTCTACACCA GCTTGTCACA 
ATCATGAATC CTAATACACT CATGGCCCAT GGAGTACCAA TTTACAGAAC CAATCAGTGC 
GCAGGAGAGT TTGTCATTAC TTTTCCGCGA TCCTATCACA GTGGCTTCAA CCAGGGCTTC 
AACTTTGCAG AGGCTGTGAA CTTTTGCACA GTGGACTGGA TGCCACTGGG GCGTCAGTGT 
GTGGATCACT ACCGCCAGCT GCATCGCTAC TGTGTGTTTT CTCATGATGA GATGGTGTGT 
AATATGGCCA TGAAGGCTGA CTGTCTTGAT GTGGTTCTGG CGTCAGCCGT ACAGAAGGAC 
ATGCAGCTCA TGATCAAAGA AGAGAGAGAG CTGCGGGAGA AAGTCCGTAA GATGGGGGTG 
GCCCAGTGCG AGTTGTTTCA ATATGACTTG CTGGCAGATG ATGAGAGACA GTGTGTGAAG 
TGTCGCACCA CCTGTTACCT GTCTGCTCTC ACCTGTCCCT GCAGACCAGG AGTCCAGGTG 
TGTCTGTACC ACACCCACGA CCTCTGCTCC TGCCCTATCT CCAACTACAC TCTCAATTAT 
CGTTTCACAC TGGATGACCT TTACCCGATG ATGAACGCTG TGCGGCAGAG GGCAGAGTAT 
TACGATGATT GGGCCTCAAG AGTGACAGAG GTCATGGAAG CAAAGCTAGA CAAGAAACGC 
AATGTCACTG TGTTTCGCAC ACTTCTGGAG GAGTCCAATG AGCAGTCGTT CCCAGAGAAT 
GACCTGTTAC GTCAGCTGAG GCTGGTTACT CAAGATGCTG AGAAATGCTC GTCTGTTGCC 
CAGCAACTGC TAAATGGCAA GCGGCAGACC AGGTACCGCA CAGGCAAGGC TAAATCTCCT 
AATCAGCTCA CAGTGGAGGA AATGAGATCA TTCGTCCGCC AGCTTTATAA CCTCCCCTGC 
AGCCTGACAC AAGCACCGCT TCTCAAGGAG CTGCTTAACA GCATAGAGGA TTTCCAGCAG 
CACAGTGAGA AGCTTCTATC TGATGAAGTG AGCGCAGATG CAGTGAGCGA GATCGAGTCT 
TTGCTGGAGG AAGGCTCTCA GTTCGATGTG TTTCTTCCGG AGCTGCCGCT GCTACGGGAG 
CGTCTGGAAC AGGCTCGATG GCTCACCGGA GTTCACCAAG CAGAGGATCC CGTAGCAAAC 
CCCTGCGGTC TCTCTCTGGA GAGCATGCGG AGGCTGATCG ACCGAGGGGT TGGACTGACG 
CCTCATCCCT CCATAGAACG CATGATGGCT CGATTGCAGG AGCTGCTTAC CGTCTCAGAG 
GAGCTGGAGG AGAATGCGCA GGCTCTGCTG AAGGCCAGAC CTCCAGAGAG TTTAGAGACA 
CTCTGCTCCA TGTTGACTCA GGTGGAAGGT GTACCTGCAT ACCTACCAAA CTGCCTCCTA 
CTACAGGACA CAGTGAACAG GGCTAAAGAA TGGCTTCAAG AAGCAGAGAG TTTACAGGTG 
GGAGGTCAGG TGCCGGTTTT CAGCTCGCTC TCTGATATGG TTCTGAGAGC TCATTCTATT 
CCTGTGCGAC TGGAGCCCCT GGACCAGCTG GAGGTTCAGG TATCAGAGGT GCAGTCCTGG 
AAGGAGACGG CAGCTAAAAC CTTCCTAATC AAGAGCTCCC CCTTCACGCT GCTGGAGGTC 
TTATGTCCTC GATGGGAAAT GGGCAGCTCT TTGAAAAAGA AGAAGATGAG AAAGATGAAG 
GGCGACTGTG TGTCATCAGG GAAGAAGAAG TTTGTGAAGC TGGACAGTAT GAGTGACGTC 
GAGAGAGCAC TTTCAGACAG TAAAGACTCT GCATCTGCTA TGTTTACTTT GGCTGAGGTG 
CGTTTAAAAG AGCTGGAGTC ACTCTGCTCT CTGCGTGCAT CGAATGAATC CAAGCTCCTG 
CCCACAGCCG ACTGCGCCTC GCTCAAAGTG TGTGTGTGTC AGAAACCAGC CATGGGCGCC 
ATGCTGCAGT GTGAGCTGTG CCGGGACGCT TTCCACAGCG TGTGTGTGCG GGGTCCCTCA 
GACCCCCTGG ACCCTGAAGC GTGGCTTTGT CCATTATGCC TGAGGTCGAC CAAACCGCCT 
CTGGATAAGA TCAGGAGCCT GCTGTCTTCA CTGCAGCGGA TCCGCGTGCG CTTACCTGAA 
GGCGATGCTT TACGTTATAT GATCGAGCGC AGTGTGAGCT GGCAGCGGAG GGTGCGAGAG 
GTTATAGACT CTTATGGGCT CTCGTCAACC TCTCAGGATG GCAGAGGAGC TTCACCTTCA 
CAGAACCTTT ACAGGAGCAC TGGCCACAGC AGGAAGCTGC AGCTGTGTGG CTCTCCATCT 
CGGTGTCAGG ACTGGGCAGT TTCAGGGCAG GAGCAGACGG TGTTTTACAC AGAACAGCGC 
TGTATTCCTC TCCAGGGTCT CAGTCCAGAG CTGGAGGAGC TGATGGTGGA GGGTTTGGTC 
CTGCAGGTTT CTTTGCCTGA GACCCAGCAG CTCTACAGGC TTCTGCTGTC TGGCCCGCCA 
ACCACAAACA CGTCCCACGC AGAACACAAG TCCTACCTCA CACCACCACA GACAGAGACA 
GATGCAATCA CTTCAGCAGA AAAGAAAGCC AAGCGGCGAA TGAACCGAGA CGAGGTGGAT 
ATCAGGGAAA GAGGGACGAA GCTGAAAAGC AAGAAGCAGA GGATGATGGG TGTGGAGAAG 
CGGAGAGAGA GGAAAGCAGC GTCAGTCTCT GCGTCAGACA TGTCTCAGTC AGAAGACTCA 
GAGGAGGACA TGACTCTGTG TCCTGCAGAG AGCTGTCTCC AGCCTGAGGG AGAAGAGGTG 
GACTGGGTGC AGTGTGATTG CTGTAACCGC TGGTTTCATA TGATTTGTGT GGGAGTCTCA 
GCAGAACTGG CAGCCGAGGA GGATTACATG TGTGTGAGCT GCAGCACAAG TCACATGGAC 
CGCCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212801.1
CDS	2490..6641
Translation	MTMGFAPGKAVGSHLRAHYERILYPYNLFQSGTNLLAPEFAVKLTGFGEPPVLEECLQKPGDEVNDIDKEYKPHDLLQRQNVPLQPSNTSAPARRAKRMKTESGCIKSEEGEGVENKPNLRRRMGSFVVKTEQKKEIPIQVKEEPVEIKELNPEPEKSKPKKKNIPPPPVSMVDLYVCLVCGKGNDEDRLLLCDGCDDSYHTFCLIPPLTDVPKGDWRCPKCLTQECCKPQEAFGFEQAHRDYTLKAFGEMADSFKSDYFNMPVHMVPTELVEKEFWRLVGTIQEDVTVEYGADIASKEFGSGFPIKGGRFKIAPHDEKYLQCGWNLNNMAMMTPSVLTHVTADICGMTLPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGAPGFAAEQLEAVMKKLAPELFDSQPDLLHQLVTIMNPNTLMAHGVPIYRTNQCAGEFVITFPRSYHSGFNQGFNFAEAVNFCTVDWMPLGRQCVDHYRQLHRYCVFSHDEMVCNMAMKADCLDVVLASAVQKDMQLMIKEERELREKVRKMGVAQCELFQYDLLADDERQCVKCRTTCYLSALTCPCRPGVQVCLYHTHDLCSCPISNYTLNYRFTLDDLYPMMNAVRQRAEYYDDWASRVTEVMEAKLDKKRNVTVFRTLLEESNEQSFPENDLLRQLRLVTQDAEKCSSVAQQLLNGKRQTRYRTGKAKSPNQLTVEEMRSFVRQLYNLPCSLTQAPLLKELLNSIEDFQQHSEKLLSDEVSADAVSEIESLLEEGSQFDVFLPELPLLRERLEQARWLTGVHQAEDPVANPCGLSLESMRRLIDRGVGLTPHPSIERMMARLQELLTVSEELEENAQALLKARPPESLETLCSMLTQVEGVPAYLPNCLLLQDTVNRAKEWLQEAESLQVGGQVPVFSSLSDMVLRAHSIPVRLEPLDQLEVQVSEVQSWKETAAKTFLIKSSPFTLLEVLCPRWEMGSSLKKKKMRKMKGDCVSSGKKKFVKLDSMSDVERALSDSKDSASAMFTLAEVRLKELESLCSLRASNESKLLPTADCASLKVCVCQKPAMGAMLQCELCRDAFHSVCVRGPSDPLDPEAWLCPLCLRSTKPPLDKIRSLLSSLQRIRVRLPEGDALRYMIERSVSWQRRVREVIDSYGLSSTSQDGRGASPSQNLYRSTGHSRKLQLCGSPSRCQDWAVSGQEQTVFYTEQRCIPLQGLSPELEELMVEGLVLQVSLPETQQLYRLLLSGPPTTNTSHAEHKSYLTPPQTETDAITSAEKKAKRRMNRDEVDIRERGTKLKSKKQRMMGVEKRRERKAASVSASDMSQSEDSEEDMTLCPAESCLQPEGEEVDWVQCDCCNRWFHMICVGVSAELAAEEDYMCVSCSTSHMDRRK

Target ORF information:

RefSeq Version	XM_017357312.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 5Bb (kdm5bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357312.1

ORF Insert Sequence:

ATGACGATGG GTTTTGCCCC AGGCAAAGCT GTGGGCTCCC ACCTGAGAGC CCATTACGAG 
AGGATCCTGT ACCCGTACAA CCTCTTTCAG TCTGGGACAA ATCTGCTGGC TCCTGAATTT 
GCAGTCAAAC TGACAGGTTT TGGGGAGCCT CCTGTGCTGG AGGAGTGTCT GCAGAAGCCT 
GGGGATGAGG TGAATGACAT TGACAAAGAG TATAAACCCC ACGACCTACT GCAGAGGCAA 
AATGTTCCAC TTCAGCCTAG CAACACGAGC GCACCGGCTA GACGAGCCAA GAGGATGAAA 
ACAGAGTCTG GCTGTATTAA GTCAGAGGAA GGTGAGGGGG TTGAGAACAA GCCAAATCTC 
AGGAGGAGGA TGGGTTCTTT CGTTGTCAAG ACTGAACAAA AAAAGGAGAT CCCTATACAG 
GTGAAAGAGG AACCTGTGGA GATAAAAGAG CTGAATCCTG AACCGGAGAA AAGCAAACCG 
AAGAAGAAAA ACATCCCCCC TCCTCCAGTC AGCATGGTGG ACCTCTATGT GTGTTTAGTG 
TGTGGAAAGG GAAACGACGA GGATCGGTTG TTGCTATGCG ACGGTTGTGA TGACAGCTAC 
CACACATTCT GCTTAATCCC GCCCCTAACC GATGTTCCGA AAGGTGATTG GCGGTGCCCT 
AAATGTCTGA CTCAGGAGTG CTGTAAACCT CAGGAGGCAT TTGGCTTCGA GCAAGCACAC 
AGAGACTATA CTCTCAAAGC CTTTGGGGAA ATGGCAGACT CCTTCAAGTC AGATTATTTC 
AACATGCCTG TTCATATGGT ACCCACTGAA TTAGTGGAAA AAGAGTTCTG GAGGTTGGTC 
GGCACCATTC AGGAGGATGT GACAGTGGAA TATGGCGCTG ACATTGCCTC AAAAGAGTTC 
GGAAGTGGGT TTCCAATCAA AGGTGGAAGA TTTAAGATTG CACCGCACGA TGAGAAATAT 
CTTCAGTGTG GTTGGAACCT GAACAATATG GCAATGATGA CACCATCAGT GCTGACACAC 
GTCACTGCTG ATATCTGTGG AATGACTTTG CCATGGCTGT ATGTGGGCAT GTGCTTCTCA 
TCGTTCTGCT GGCACATTGA GGACCACTGG AGCTATTCCA TCAACTACCT GCACTGGGGA 
GAACCTAAGA CCTGGTATGG TGCTCCGGGT TTTGCTGCTG AGCAGTTGGA AGCCGTCATG 
AAGAAACTGG CCCCAGAACT ATTTGATTCT CAGCCCGACC TTCTACACCA GCTTGTCACA 
ATCATGAATC CTAATACACT CATGGCCCAT GGAGTACCAA TTTACAGAAC CAATCAGTGC 
GCAGGAGAGT TTGTCATTAC TTTTCCGCGA TCCTATCACA GTGGCTTCAA CCAGGGCTTC 
AACTTTGCAG AGGCTGTGAA CTTTTGCACA GTGGACTGGA TGCCACTGGG GCGTCAGTGT 
GTGGATCACT ACCGCCAGCT GCATCGCTAC TGTGTGTTTT CTCATGATGA GATGGTGTGT 
AATATGGCCA TGAAGGCTGA CTGTCTTGAT GTGGTTCTGG CGTCAGCCGT ACAGAAGGAC 
ATGCAGCTCA TGATCAAAGA AGAGAGAGAG CTGCGGGAGA AAGTCCGTAA GATGGGGGTG 
GCCCAGTGCG AGTTGTTTCA ATATGACTTG CTGGCAGATG ATGAGAGACA GTGTGTGAAG 
TGTCGCACCA CCTGTTACCT GTCTGCTCTC ACCTGTCCCT GCAGACCAGG AGTCCAGGTG 
TGTCTGTACC ACACCCACGA CCTCTGCTCC TGCCCTATCT CCAACTACAC TCTCAATTAT 
CGTTTCACAC TGGATGACCT TTACCCGATG ATGAACGCTG TGCGGCAGAG GGCAGAGTAT 
TACGATGATT GGGCCTCAAG AGTGACAGAG GTCATGGAAG CAAAGCTAGA CAAGAAACGC 
AATGTCACTG TGTTTCGCAC ACTTCTGGAG GAGTCCAATG AGCAGTCGTT CCCAGAGAAT 
GACCTGTTAC GTCAGCTGAG GCTGGTTACT CAAGATGCTG AGAAATGCTC GTCTGTTGCC 
CAGCAACTGC TAAATGGCAA GCGGCAGACC AGGTACCGCA CAGGCAAGGC TAAATCTCCT 
AATCAGCTCA CAGTGGAGGA AATGAGATCA TTCGTCCGCC AGCTTTATAA CCTCCCCTGC 
AGCCTGACAC AAGCACCGCT TCTCAAGGAG CTGCTTAACA GCATAGAGGA TTTCCAGCAG 
CACAGTGAGA AGCTTCTATC TGATGAAGTG AGCGCAGATG CAGTGAGCGA GATCGAGTCT 
TTGCTGGAGG AAGGCTCTCA GTTCGATGTG TTTCTTCCGG AGCTGCCGCT GCTACGGGAG 
CGTCTGGAAC AGGCTCGATG GCTCACCGGA GTTCACCAAG CAGAGGATCC CGTAGCAAAC 
CCCTGCGGTC TCTCTCTGGA GAGCATGCGG AGGCTGATCG ACCGAGGGGT TGGACTGACG 
CCTCATCCCT CCATAGAACG CATGATGGCT CGATTGCAGG AGCTGCTTAC CGTCTCAGAG 
GAGCTGGAGG AGAATGCGCA GGCTCTGCTG AAGGCCAGAC CTCCAGAGAG TTTAGAGACA 
CTCTGCTCCA TGTTGACTCA GGTGGAAGGT GTACCTGCAT ACCTACCAAA CTGCCTCCTA 
CTACAGGACA CAGTGAACAG GGCTAAAGAA TGGCTTCAAG AAGCAGAGAG TTTACAGGTG 
GGAGGTCAGG TGCCGGTTTT CAGCTCGCTC TCTGATATGG TTCTGAGAGC TCATTCTATT 
CCTGTGCGAC TGGAGCCCCT GGACCAGCTG GAGGTTCAGG TATCAGAGGT GCAGTCCTGG 
AAGGAGACGG CAGCTAAAAC CTTCCTAATC AAGAGCTCCC CCTTCACGCT GCTGGAGGTC 
TTATGTCCTC GATGGGAAAT GGGCAGCTCT TTGAAAAAGA AGAAGATGAG AAAGATGAAG 
GGCGACTGTG TGTCATCAGG GAAGAAGAAG TTTGTGAAGC TGGACAGTAT GAGTGACGTC 
GAGAGAGCAC TTTCAGACAG TAAAGACTCT GCATCTGCTA TGTTTACTTT GGCTGAGGTG 
CGTTTAAAAG AGCTGGAGTC ACTCTGCTCT CTGCGTGCAT CGAATGAATC CAAGCTCCTG 
CCCACAGCCG ACTGCGCCTC GCTCAAAGTG TGTGTGTGTC AGAAACCAGC CATGGGCGCC 
ATGCTGCAGT GTGAGCTGTG CCGGGACGCT TTCCACAGCG TGTGTGTGCG GGGTCCCTCA 
GACCCCCTGG ACCCTGAAGC GTGGCTTTGT CCATTATGCC TGAGGTCGAC CAAACCGCCT 
CTGGATAAGA TCAGGAGCCT GCTGTCTTCA CTGCAGCGGA TCCGCGTGCG CTTACCTGAA 
GGCGATGCTT TACGTTATAT GATCGAGCGC AGTGTGAGCT GGCAGCGGAG GGTGCGAGAG 
GTTATAGACT CTTATGGGCT CTCGTCAACC TCTCAGGATG GCAGAGGAGC TTCACCTTCA 
CAGAACCTTT ACAGGAGCAC TGGCCACAGC AGGAAGCTGC AGCTGTGTGG CTCTCCATCT 
CGGTGTCAGG ACTGGGCAGT TTCAGGGCAG GAGCAGACGG TGTTTTACAC AGAACAGCGC 
TGTATTCCTC TCCAGGGTCT CAGTCCAGAG CTGGAGGAGC TGATGGTGGA GGGTTTGGTC 
CTGCAGGTTT CTTTGCCTGA GACCCAGCAG CTCTACAGGC TTCTGCTGTC TGGCCCGCCA 
ACCACAAACA CGTCCCACGC AGAACACAAG TCCTACCTCA CACCACCACA GACAGAGACA 
GATGCAATCA CTTCAGCAGA AAAGAAAGCC AAGCGGCGAA TGAACCGAGA CGAGGTGGAT 
ATCAGGGAAA GAGGGACGAA GCTGAAAAGC AAGAAGCAGA GGATGATGGG TGTGGAGAAG 
CGGAGAGAGA GGAAAGCAGC GTCAGTCTCT GCGTCAGACA TGTCTCAGTC AGAAGACTCA 
GAGGAGGACA TGACTCTGTG TCCTGCAGAG AGCTGTCTCC AGCCTGAGGG AGAAGAGGTG 
GACTGGGTGC AGTGTGATTG CTGTAACCGC TGGTTTCATA TGATTTGTGT GGGAGTCTCA 
GCAGAACTGG CAGCCGAGGA GGATTACATG TGTGTGAGCT GCAGCACAAG TCACATGGAC 
CGCCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhancer activity-based identification of functional enhancers using zebrafish embryos.
Genomics108(2)102-7(2016 08)
Taminato T,Yokota D,Araki S,Ovara H,Yamasu K,Kawamura A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

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kdm5bb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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