rap1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rap1aa
Entrez Gene ID	415242
Full Name	RAP1A, member of RAS oncogene family a
Synonyms	rap1a,wu:fc66g06,zgc:86676
General protein information	Preferred Names RAP1A, member of RAS oncogene family a Names ras-related protein Rap-1A Rap1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002152.1	NP_001002152.1	ras-related protein Rap-1A
XM_005167023.2	XP_005167080.1	ras-related protein Rap-1A isoform X1
XM_005167023.3	XP_005167080.1	ras-related protein Rap-1A isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RAP1A	XP_524793.1
Mus musculus (house mouse)	Rap1a	NP_663516.1
Homo sapiens (human)	RAP1A	NP_001010935.1
Danio rerio (zebrafish)	rap1ab	NP_001230108.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001874	XP_003435963.1
Rattus norvegicus (Norway rat)	Rap1a	NP_001005765.1
Drosophila melanogaster (fruit fly)	R	NP_001189023.1
Eremothecium gossypii	AGOS_AFR464W	NP_986011.1
Magnaporthe oryzae (rice blast fungus)	MGG_02727	XP_003721031.1
	RAP1A	XP_001106027.1
Bos taurus (cattle)	RAP1A	NP_776873.1
Gallus gallus (chicken)	RAP1A	XP_417994.1
Neurospora crassa	NCU02167	XP_964526.2
Xenopus tropicalis (tropical clawed frog)	rap1a	NP_001096357.1
Canis lupus familiaris (dog)	RAP1A	XP_863066.1
Danio rerio (zebrafish)	rap1aa	NP_001002152.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.
B?gershausen N, Tsai IC, Pohl E, Kiper P?, Beleggia F, Percin EF, Keupp K, Matchan A, Milz E, Alanay Y, Kayserili H, Liu Y, Banka S, Kranz A, Zenker M, Wieczorek D, Elcioglu N, Prontera P, Lyonnet S, Meitinger T, Stewart AF, Donnai D, Strom TM, Boduroglu K, Yigit G, Li Y, Katsanis N, Wollnik B
The Journal of clinical investigation125(9)3585-99(2015 Sep)

Suppression of Rap1 impairs cardiac myofibrils and conduction system in zebrafish.
Dong W, Yang Z, Yang F, Wang J, Zhuang Y, Xu C, Zhang B, Tian XL, Liu D
PloS one7(11)e50960(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.
Title: RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.

The following rap1aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rap1aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00498	NM_001002152.1 Latest version!	Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa00498	XM_005167023.3 Latest version!	Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa00498	XM_005167023.2	Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rap1aa ( NM_001002152.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002152.1 Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), mRNA.

Price & Availability↑

CloneID	ODa00498
Clone ID Related Accession (Same CDS sequence)	NM_001002152.1 , XM_005167023.2 , XM_005167023.3
Accession Version	NM_001002152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	ras-related protein Rap-1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071360.1. ##Evidence-Data-START## Transcript exon combination :: BC071360.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGTGAAT ATAAGCTTGT GGTCTTGGGC TCTGGCGGCG TAGGAAAATC TGCACTGACG 
GTCCAGTTTG TACAGGGAAT ATTTGTTGAA AAATATGACC CCACAATAGA AGACTCATAT 
AGAAAGCAAG TTGAGGTTGA TGGGCAGCAA TGTATGCTAG AGATCCTGGA CACTGCAGGC 
ACAGAGCAGT TCACGGCGAT GAGGGACCTG TACATGAAGA ATGGCCAGGG TTTTGCTCTG 
GTTTACTCCA TCACAGCACA GTCCACCTTT AATGATCTGC AGGACCTGCG GGAGCAGATT 
CTGAGGGTGA AGGACACCGA GGATGTGCCA ATGATTTTGG TTGGAAACAA GTGTGATTTA 
GAAGAAGAGA GGGTGGTTGG AAAAGAGCAG GGTCAGAATC TTGCCCGTCA GTGGAGTAAT 
TGTGCCTTTC TAGAATCTTC TGCCAAATCA AAGATCAACG TCAATGAGAT CTTTTATGAC 
CTGGTTAGAC AGATAAACAG AAAGACACCG GTGGAGAAGA AGAGAGCAAA AAAGAAGTCA 
AACTGTGTCC TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002152.1
CDS	112..669
Translation	MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEEERVVGKEQGQNLARQWSNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKRAKKKSNCVLL

Target ORF information:

RefSeq Version	NM_001002152.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002152.1

ORF Insert Sequence:

ATGCGTGAAT ATAAGCTTGT GGTCTTGGGC TCTGGCGGCG TAGGAAAATC TGCACTGACG 
GTCCAGTTTG TACAGGGAAT ATTTGTTGAA AAATATGACC CCACAATAGA AGACTCATAT 
AGAAAGCAAG TTGAGGTTGA TGGGCAGCAA TGTATGCTAG AGATCCTGGA CACTGCAGGC 
ACAGAGCAGT TCACGGCGAT GAGGGACCTG TACATGAAGA ATGGCCAGGG TTTTGCTCTG 
GTTTACTCCA TCACAGCACA GTCCACCTTT AATGATCTGC AGGACCTGCG GGAGCAGATT 
CTGAGGGTGA AGGACACCGA GGATGTGCCA ATGATTTTGG TTGGAAACAA GTGTGATTTA 
GAAGAAGAGA GGGTGGTTGG AAAAGAGCAG GGTCAGAATC TTGCCCGTCA GTGGAGTAAT 
TGTGCCTTTC TAGAATCTTC TGCCAAATCA AAGATCAACG TCAATGAGAT CTTTTATGAC 
CTGGTTAGAC AGATAAACAG AAAGACACCG GTGGAGAAGA AGAGAGCAAA AAAGAAGTCA 
AACTGTGTCC TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rap1aa ( XM_005167023.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167080.1 Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00498
Clone ID Related Accession (Same CDS sequence)	NM_001002152.1 , XM_005167023.2 , XM_005167023.3
Accession Version	XM_005167023.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ras-related protein Rap-1A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167023.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAAT ATAAGCTTGT GGTCTTGGGC TCTGGCGGCG TAGGAAAATC TGCACTGACG 
GTCCAGTTTG TACAGGGAAT ATTTGTTGAA AAATATGACC CCACAATAGA AGACTCATAT 
AGAAAGCAAG TTGAGGTTGA TGGGCAGCAA TGTATGCTAG AGATCCTGGA CACTGCAGGC 
ACAGAGCAGT TCACGGCGAT GAGGGACCTG TACATGAAGA ATGGCCAGGG TTTTGCTCTG 
GTTTACTCCA TCACAGCACA GTCCACCTTT AATGATCTGC AGGACCTGCG GGAGCAGATT 
CTGAGGGTGA AGGACACCGA GGATGTGCCA ATGATTTTGG TTGGAAACAA GTGTGATTTA 
GAAGAAGAGA GGGTGGTTGG AAAAGAGCAG GGTCAGAATC TTGCCCGTCA GTGGAGTAAT 
TGTGCCTTTC TAGAATCTTC TGCCAAATCA AAGATCAACG TCAATGAGAT CTTTTATGAC 
CTGGTTAGAC AGATAAACAG AAAGACACCG GTGGAGAAGA AGAGAGCAAA AAAGAAGTCA 
AACTGTGTCC TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167080.1
CDS	172..729
Translation	MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEEERVVGKEQGQNLARQWSNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKRAKKKSNCVLL

Target ORF information:

RefSeq Version	XM_005167023.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167023.2

ORF Insert Sequence:

ATGCGTGAAT ATAAGCTTGT GGTCTTGGGC TCTGGCGGCG TAGGAAAATC TGCACTGACG 
GTCCAGTTTG TACAGGGAAT ATTTGTTGAA AAATATGACC CCACAATAGA AGACTCATAT 
AGAAAGCAAG TTGAGGTTGA TGGGCAGCAA TGTATGCTAG AGATCCTGGA CACTGCAGGC 
ACAGAGCAGT TCACGGCGAT GAGGGACCTG TACATGAAGA ATGGCCAGGG TTTTGCTCTG 
GTTTACTCCA TCACAGCACA GTCCACCTTT AATGATCTGC AGGACCTGCG GGAGCAGATT 
CTGAGGGTGA AGGACACCGA GGATGTGCCA ATGATTTTGG TTGGAAACAA GTGTGATTTA 
GAAGAAGAGA GGGTGGTTGG AAAAGAGCAG GGTCAGAATC TTGCCCGTCA GTGGAGTAAT 
TGTGCCTTTC TAGAATCTTC TGCCAAATCA AAGATCAACG TCAATGAGAT CTTTTATGAC 
CTGGTTAGAC AGATAAACAG AAAGACACCG GTGGAGAAGA AGAGAGCAAA AAAGAAGTCA 
AACTGTGTCC TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rap1aa ( XM_005167023.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167080.1 Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00498
Clone ID Related Accession (Same CDS sequence)	NM_001002152.1 , XM_005167023.2 , XM_005167023.3
Accession Version	XM_005167023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ras-related protein Rap-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTGAAT ATAAGCTTGT GGTCTTGGGC TCTGGCGGCG TAGGAAAATC TGCACTGACG 
GTCCAGTTTG TACAGGGAAT ATTTGTTGAA AAATATGACC CCACAATAGA AGACTCATAT 
AGAAAGCAAG TTGAGGTTGA TGGGCAGCAA TGTATGCTAG AGATCCTGGA CACTGCAGGC 
ACAGAGCAGT TCACGGCGAT GAGGGACCTG TACATGAAGA ATGGCCAGGG TTTTGCTCTG 
GTTTACTCCA TCACAGCACA GTCCACCTTT AATGATCTGC AGGACCTGCG GGAGCAGATT 
CTGAGGGTGA AGGACACCGA GGATGTGCCA ATGATTTTGG TTGGAAACAA GTGTGATTTA 
GAAGAAGAGA GGGTGGTTGG AAAAGAGCAG GGTCAGAATC TTGCCCGTCA GTGGAGTAAT 
TGTGCCTTTC TAGAATCTTC TGCCAAATCA AAGATCAACG TCAATGAGAT CTTTTATGAC 
CTGGTTAGAC AGATAAACAG AAAGACACCG GTGGAGAAGA AGAGAGCAAA AAAGAAGTCA 
AACTGTGTCC TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167080.1
CDS	172..729
Translation	MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDGQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEEERVVGKEQGQNLARQWSNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKRAKKKSNCVLL

Target ORF information:

RefSeq Version	XM_005167023.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RAP1A, member of RAS oncogene family a (rap1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167023.3

ORF Insert Sequence:

ATGCGTGAAT ATAAGCTTGT GGTCTTGGGC TCTGGCGGCG TAGGAAAATC TGCACTGACG 
GTCCAGTTTG TACAGGGAAT ATTTGTTGAA AAATATGACC CCACAATAGA AGACTCATAT 
AGAAAGCAAG TTGAGGTTGA TGGGCAGCAA TGTATGCTAG AGATCCTGGA CACTGCAGGC 
ACAGAGCAGT TCACGGCGAT GAGGGACCTG TACATGAAGA ATGGCCAGGG TTTTGCTCTG 
GTTTACTCCA TCACAGCACA GTCCACCTTT AATGATCTGC AGGACCTGCG GGAGCAGATT 
CTGAGGGTGA AGGACACCGA GGATGTGCCA ATGATTTTGG TTGGAAACAA GTGTGATTTA 
GAAGAAGAGA GGGTGGTTGG AAAAGAGCAG GGTCAGAATC TTGCCCGTCA GTGGAGTAAT 
TGTGCCTTTC TAGAATCTTC TGCCAAATCA AAGATCAACG TCAATGAGAT CTTTTATGAC 
CTGGTTAGAC AGATAAACAG AAAGACACCG GTGGAGAAGA AGAGAGCAAA AAAGAAGTCA 
AACTGTGTCC TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

RAP1-mediated MEK/ERK pathway defects in Kabuki syndrome.
The Journal of clinical investigation125(9)3585-99(2015 Sep)
B?gershausen N,Tsai IC,Pohl E,Kiper P?,Beleggia F,Percin EF,Keupp K,Matchan A,Milz E,Alanay Y,Kayserili H,Liu Y,Banka S,Kranz A,Zenker M,Wieczorek D,Elcioglu N,Prontera P,Lyonnet S,Meitinger T,Stewart AF,Donnai D,Strom TM,Boduroglu K,Yigit G,Li Y,Katsanis N,Wollnik B

Suppression of Rap1 impairs cardiac myofibrils and conduction system in zebrafish.
PloS one7(11)e50960(2012)
Dong W,Yang Z,Yang F,Wang J,Zhuang Y,Xu C,Zhang B,Tian XL,Liu D

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rap1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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