MED22 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED22
Entrez Gene ID	415234
Full Name	mediator complex subunit 22
Synonyms	si:ch73-278c20.1,si:dkey-226m8.2,surf5,wu:fj45h07,zgc:86721
General protein information	Preferred Names mediator complex subunit 22 Names mediator of RNA polymerase II transcription subunit 22 surfeit 5 surfeit locus protein 5
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005171832.4	XP_005171889.1	mediator of RNA polymerase II transcription subunit 22 isoform X1
XM_021476063.1	XP_021331738.1	mediator of RNA polymerase II transcription subunit 22 isoform X2
XM_005171832.3	XP_005171889.1	mediator of RNA polymerase II transcription subunit 22 isoform X1
NM_001002144.1	NP_001002144.1	mediator of RNA polymerase II transcription subunit 22
XM_005171831.2	XP_005171888.1	mediator of RNA polymerase II transcription subunit 22 isoform X1
XM_005171831.1	XP_005171888.1	mediator of RNA polymerase II transcription subunit 22 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED22	NP_598395.1
Bos taurus (cattle)	MED22	NP_001029914.1
Mus musculus (house mouse)	Med22	NP_001029080.1
Pan troglodytes (chimpanzee)	MED22	XP_528453.2
Gallus gallus (chicken)	MED22	NP_001039295.1
Xenopus tropicalis (tropical clawed frog)	med22	NP_001120562.1
Canis lupus familiaris (dog)	MED22	XP_005625201.1
Rattus norvegicus (Norway rat)	Med22	NP_001071147.1
Rattus norvegicus (Norway rat)	LOC100911917	XP_003749478.1
Anopheles gambiae (African malaria mosquito)	MED22_ANOGA	XP_313084.3
Danio rerio (zebrafish)	med22	NP_001002144.1
	MED22	XP_001101391.1
Drosophila melanogaster (fruit fly)	MED22	NP_569870.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Coherent but overlapping expression of microRNAs and their targets during vertebrate development.
Shkumatava A, Stark A, Sive H, Bartel DP
Genes & development23(4)466-81(2009 Feb)

Long-term evolution of the CAZY glycosyltransferase 6 (ABO) gene family from fishes to mammals--a birth-and-death evolution model.
Turcot-Dubois AL, Le Moullac-Vaidye B, Despiau S, Roubinet F, Bovin N, Le Pendu J, Blancher A
Glycobiology17(5)516-28(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38459	NM_001002144.1 Latest version!	Danio rerio mediator complex subunit 22 (med22), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa10456	XM_005171831.2 Latest version!	Danio rerio mediator complex subunit 22 (med22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa10456	XM_005171832.4 Latest version!	Danio rerio mediator complex subunit 22 (med22), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa38459	XM_021476063.1 Latest version!	Danio rerio mediator complex subunit 22 (med22), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa10456	XM_005171832.3	Danio rerio mediator complex subunit 22 (med22), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa10456	XM_005171831.1	Danio rerio mediator complex subunit 22 (med22), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED22 ( NM_001002144.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001002144.1 Danio rerio mediator complex subunit 22 (med22), mRNA.

Price & Availability↑

CloneID	ODa38459
Clone ID Related Accession (Same CDS sequence)	XM_021476063.1 , NM_001002144.1
Accession Version	NM_001002144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 22
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071397.1. On or before Jul 6, 2005 this sequence version replaced XM_702212.1, XM_678840.1, XM_702213.1, XM_702214.1. ##Evidence-Data-START## Transcript exon combination :: BC071397.1, EL650743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGGTACAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002144.1
CDS	59..481
Translation	MSTPRVLPQSKETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEEYYSSRYK

Target ORF information:

RefSeq Version	NM_001002144.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 22 (med22), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002144.1

ORF Insert Sequence:

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGGTACAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( XM_005171832.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171889.1 Danio rerio mediator complex subunit 22 (med22), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10456
Clone ID Related Accession (Same CDS sequence)	XM_005171832.4 , XM_005171832.3 , XM_005171831.2 , XM_005171831.1
Accession Version	XM_005171832.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171832.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171889.1
CDS	236..832
Translation	MSTPRVLPQSKETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEEYYSSSCGQWDGVELPLCEAFHRQDSWGSPEMTSDPSNANPEDSDHLGSQESMQRHRNGSGTSEQS

Target ORF information:

RefSeq Version	XM_005171832.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 22 (med22), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171832.4

ORF Insert Sequence:

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( XM_005171832.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171889.1 Danio rerio mediator complex subunit 22 (med22), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10456
Clone ID Related Accession (Same CDS sequence)	XM_005171832.4 , XM_005171832.3 , XM_005171831.2 , XM_005171831.1
Accession Version	XM_005171832.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171889.1
CDS	274..870
Translation	MSTPRVLPQSKETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEEYYSSSCGQWDGVELPLCEAFHRQDSWGSPEMTSDPSNANPEDSDHLGSQESMQRHRNGSGTSEQS

Target ORF information:

RefSeq Version	XM_005171832.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 22 (med22), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171832.3

ORF Insert Sequence:

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( XM_005171831.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171888.1 Danio rerio mediator complex subunit 22 (med22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10456
Clone ID Related Accession (Same CDS sequence)	XM_005171832.4 , XM_005171832.3 , XM_005171831.2 , XM_005171831.1
Accession Version	XM_005171831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171888.1
CDS	126..722
Translation	MSTPRVLPQSKETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEEYYSSSCGQWDGVELPLCEAFHRQDSWGSPEMTSDPSNANPEDSDHLGSQESMQRHRNGSGTSEQS

Target ORF information:

RefSeq Version	XM_005171831.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 22 (med22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171831.2

ORF Insert Sequence:

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( XM_005171831.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005171888.1 Danio rerio mediator complex subunit 22 (med22), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10456
Clone ID Related Accession (Same CDS sequence)	XM_005171832.4 , XM_005171832.3 , XM_005171831.2 , XM_005171831.1
Accession Version	XM_005171831.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171888.1
CDS	125..721
Translation	MSTPRVLPQSKETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEEYYSSSCGQWDGVELPLCEAFHRQDSWGSPEMTSDPSNANPEDSDHLGSQESMQRHRNGSGTSEQS

Target ORF information:

RefSeq Version	XM_005171831.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 22 (med22), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171831.1

ORF Insert Sequence:

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGCTGTGGA 
CAATGGGATG GTGTTGAGTT GCCACTTTGT GAGGCCTTTC ATCGGCAGGA CAGTTGGGGG 
TCCCCTGAGA TGACCTCTGA CCCCTCAAAT GCCAACCCTG AGGACTCAGA CCACTTGGGA 
TCCCAGGAGT CTATGCAACG TCACCGCAAC GGCAGTGGCA CTAGTGAGCA GTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( XM_021476063.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021331738.1 Danio rerio mediator complex subunit 22 (med22), transcript variant X3, mRNA.

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CloneID	ODa38459
Clone ID Related Accession (Same CDS sequence)	XM_021476063.1 , NM_001002144.1
Accession Version	XM_021476063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 22 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGGTACAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331738.1
CDS	236..658
Translation	MSTPRVLPQSKETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEEYYSSRYK

Target ORF information:

RefSeq Version	XM_021476063.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 22 (med22), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476063.1

ORF Insert Sequence:

ATGTCCACAC CGAGAGTCCT CCCGCAAAGC AAAGAGACTC TTTTGCAGAA CTACAACAAG 
AGACTCAAAG ATGACATCAG GTCAATTCTA GACAATTTTA CGGAAATAAT TAAAACTGCT 
AAGGTGGAAG ATGAGACTCA GGTTTCCAGG GCGACGCAAG CTGAACAGGA CCATTTTGAA 
ATGCATGTCC GAGCTGCCAA CATTGTACGT GCAGGAGAGT CTCTGATGAA GCTTGTCTCA 
GACCTGAAGC AGTTCCTTAT CCTGAATGAT TTCCCGTCTG TCAACGAGGC CATCAGTCTG 
CGTAACCAGC AGCTCCGCAC ACTGCAGGAG GAGTGCGACA AGAAGCTCAT CTCTTTGCGG 
GATGAGATTG CCATCGACCT GTATGAGCTC GAGGAAGAGT ATTACTCCTC CAGGTACAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Coherent but overlapping expression of microRNAs and their targets during vertebrate development.
Genes & development23(4)466-81(2009 Feb)
Shkumatava A,Stark A,Sive H,Bartel DP

Long-term evolution of the CAZY glycosyltransferase 6 (ABO) gene family from fishes to mammals--a birth-and-death evolution model.
Glycobiology17(5)516-28(2007 May)
Turcot-Dubois AL,Le Moullac-Vaidye B,Despiau S,Roubinet F,Bovin N,Le Pendu J,Blancher A

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MED22 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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