msrb3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	msrb3
Entrez Gene ID	415184
Full Name	methionine sulfoxide reductase B3
Synonyms	wu:fc07g04,zgc:86909
General protein information	Preferred Names methionine sulfoxide reductase B3 Names methionine-R-sulfoxide reductase B3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021474986.1	XP_021330661.1	methionine-R-sulfoxide reductase B3 isoform X2
NM_001320380.1	NP_001307309.1	methionine-R-sulfoxide reductase B3 isoform 3 precursor
XM_009300117.2	XP_009298392.1	methionine-R-sulfoxide reductase B3 isoform X1
NM_001002094.2	NP_001002094.2	methionine-R-sulfoxide reductase B3 isoform 2
XM_009300117.1	XP_009298392.1	methionine-R-sulfoxide reductase B3 isoform X1
NM_001320378.1	NP_001307307.1	methionine-R-sulfoxide reductase B3 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MSRB3	NP_001026849.1
Pan troglodytes (chimpanzee)	MSRB3	XP_001163219.1
Bos taurus (cattle)	MSRB3	XP_002687589.1
	MSRB3	XP_001117006.1
Mus musculus (house mouse)	Msrb3	NP_796066.1
Danio rerio (zebrafish)	msrb3	NP_001002094.1
Canis lupus familiaris (dog)	MSRB3	XP_850508.3
Rattus norvegicus (Norway rat)	Msrb3	XP_006241476.1
Arabidopsis thaliana (thale cress)	MSRB1	NP_564640.2
Gallus gallus (chicken)	MSRB3	NP_001186507.1
Drosophila melanogaster (fruit fly)	SelR	NP_731522.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

Novel Genes Critical for Hypoxic Preconditioning in Zebrafish Are Regulators of Insulin and Glucose Metabolism.
Manchenkov T, Pasillas MP, Haddad GG, Imam FB
G3 (Bethesda, Md.)5(6)1107-16(2015 Apr)

Down-regulation of msrb3 and destruction of normal auditory system development through hair cell apoptosis in zebrafish.
Shen X, Liu F, Wang Y, Wang H, Ma J, Xia W, Zhang J, Jiang N, Sun S, Wang X, Ma D
The International journal of developmental biology59(4-6)195-203(2015)

Porcine methionine sulfoxide reductase B3: molecular cloning, tissue-specific expression profiles, and polymorphisms associated with ear size in Sus scrofa.
Zhang Y, Liang J, Zhang L, Wang L, Liu X, Yan H, Zhao K, Shi H, Zhang T, Li N, Pu L, Wang L
Journal of animal science and biotechnology660(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that methionine sulfoxide reductase B3 (msrb3) is instrumental for auditory system development in zebrafish.
Title: Down-regulation of msrb3 and destruction of normal auditory system development through hair cell apoptosis in zebrafish.

The following msrb3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msrb3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09238	NM_001320378.1 Latest version!	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa09239	NM_001320380.1 Latest version!	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa09240	NM_001002094.2 Latest version!	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa09238	XM_009300117.2 Latest version!	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa09240	XM_021474986.1 Latest version!	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
ODa09238	XM_009300117.1	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msrb3 ( NM_001320378.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001307307.1 Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa09238
Clone ID Related Accession (Same CDS sequence)	XM_009300117.2 , XM_009300117.1 , NM_001320378.1
Accession Version	NM_001320378.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	methionine-R-sulfoxide reductase B3 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX119963.5. On Feb 23, 2016 this sequence version replaced XM_005164619.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by partial transcript alignments and orthologous alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01026987.1, GDQQ01000426.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTGTCC GTCTTCTCCG ATCCATGGCC CCGTGTGCAT CCATGGCCCT GCTCCAATGC 
ATGCGCTCCC ATAAGACCAC ACGACCACTC CTAATACTGA CTACACCCGC TGCGCTAGGA 
ACGTGCCGAA GTAAGAAGAC ATGGCCCAAA ACGTTCCCCG AGGAGGAGCT GAGGAAGCGT 
TTGACCCCAA TGCAGTACCA TGTTACTCAG CAGAAGGGCA CAGAGAGCGC ATTCACAGGA 
AAATATGTCG ATAATAAAGA GGAGGGAACT TACCACTGTG TTGTTTGCGG TGCACCTCTT 
TTCATGTCTG AAAAGAAGTT TGACTCAGGA TCAGGATGGC CTTCTTTTTT CGATTTGATC 
AATAAGGACG CTATAGCTCA AACAGATGAC TTTTCATACG GAATGCACAG AGTGGAGACA 
ACCTGCAGTC AGTGTGGTGC TCACCTGGGC CACCTCTTCG ATGATGGACC TCGACCAACA 
AAGAAACGCT ACTGCATAAA CTCGGCCTCA CTCAGTTTCC AGCCCAAAAA CAGTGCCACT 
GAGGAAGTGG AGGCATCTGG AGCAGCCGGT CAATCATCCA CTAGCAAGAA TGAAGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307307.1
CDS	201..803
Translation	MAVRLLRSMAPCASMALLQCMRSHKTTRPLLILTTPAALGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVEASGAAGQSSTSKNEEL

Target ORF information:

RefSeq Version	NM_001320378.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320378.1

ORF Insert Sequence:

ATGGCTGTCC GTCTTCTCCG ATCCATGGCC CCGTGTGCAT CCATGGCCCT GCTCCAATGC 
ATGCGCTCCC ATAAGACCAC ACGACCACTC CTAATACTGA CTACACCCGC TGCGCTAGGA 
ACGTGCCGAA GTAAGAAGAC ATGGCCCAAA ACGTTCCCCG AGGAGGAGCT GAGGAAGCGT 
TTGACCCCAA TGCAGTACCA TGTTACTCAG CAGAAGGGCA CAGAGAGCGC ATTCACAGGA 
AAATATGTCG ATAATAAAGA GGAGGGAACT TACCACTGTG TTGTTTGCGG TGCACCTCTT 
TTCATGTCTG AAAAGAAGTT TGACTCAGGA TCAGGATGGC CTTCTTTTTT CGATTTGATC 
AATAAGGACG CTATAGCTCA AACAGATGAC TTTTCATACG GAATGCACAG AGTGGAGACA 
ACCTGCAGTC AGTGTGGTGC TCACCTGGGC CACCTCTTCG ATGATGGACC TCGACCAACA 
AAGAAACGCT ACTGCATAAA CTCGGCCTCA CTCAGTTTCC AGCCCAAAAA CAGTGCCACT 
GAGGAAGTGG AGGCATCTGG AGCAGCCGGT CAATCATCCA CTAGCAAGAA TGAAGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msrb3 ( NM_001320380.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001307309.1 Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ODa09239
Clone ID Related Accession (Same CDS sequence)	NM_001320380.1
Accession Version	NM_001320380.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	methionine-R-sulfoxide reductase B3 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX119963.5. On Feb 23, 2016 this sequence version replaced XM_005164620.2. Transcript Variant: This variant (3) lacks an exon in the 5' UTR and 5' coding region which results in the use of an alternate start codon compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by partial transcript alignments and orthologous alignments. ##Evidence-Data-START## Transcript exon combination :: CF998052.1, DT058112.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476800 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCGCTGCT CTCCCCGCGG CCCCCCTTTC CTGTATACTC TGCTGTCCTG CTCGGTTATA 
ACGCTATTTC TGCTGTCCGG AACGTGCCGA AGTAAGAAGA CATGGCCCAA AACGTTCCCC 
GAGGAGGAGC TGAGGAAGCG TTTGACCCCA ATGCAGTACC ATGTTACTCA GCAGAAGGGC 
ACAGAGAGCG CATTCACAGG AAAATATGTC GATAATAAAG AGGAGGGAAC TTACCACTGT 
GTTGTTTGCG GTGCACCTCT TTTCATGTCT GAAAAGAAGT TTGACTCAGG ATCAGGATGG 
CCTTCTTTTT TCGATTTGAT CAATAAGGAC GCTATAGCTC AAACAGATGA CTTTTCATAC 
GGAATGCACA GAGTGGAGAC AACCTGCAGT CAGTGTGGTG CTCACCTGGG CCACCTCTTC 
GATGATGGAC CTCGACCAAC AAAGAAACGC TACTGCATAA ACTCGGCCTC ACTCAGTTTC 
CAGCCCAAAA ACAGTGCCAC TGAGGAAGTG GAGGCATCTG GAGCAGCCGG TCAATCATCC 
ACTAGCAAGA ATGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307309.1
CDS	53..616
Translation	MRCSPRGPPFLYTLLSCSVITLFLLSGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVEASGAAGQSSTSKNEEL

Target ORF information:

RefSeq Version	NM_001320380.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320380.1

ORF Insert Sequence:

ATGCGCTGCT CTCCCCGCGG CCCCCCTTTC CTGTATACTC TGCTGTCCTG CTCGGTTATA 
ACGCTATTTC TGCTGTCCGG AACGTGCCGA AGTAAGAAGA CATGGCCCAA AACGTTCCCC 
GAGGAGGAGC TGAGGAAGCG TTTGACCCCA ATGCAGTACC ATGTTACTCA GCAGAAGGGC 
ACAGAGAGCG CATTCACAGG AAAATATGTC GATAATAAAG AGGAGGGAAC TTACCACTGT 
GTTGTTTGCG GTGCACCTCT TTTCATGTCT GAAAAGAAGT TTGACTCAGG ATCAGGATGG 
CCTTCTTTTT TCGATTTGAT CAATAAGGAC GCTATAGCTC AAACAGATGA CTTTTCATAC 
GGAATGCACA GAGTGGAGAC AACCTGCAGT CAGTGTGGTG CTCACCTGGG CCACCTCTTC 
GATGATGGAC CTCGACCAAC AAAGAAACGC TACTGCATAA ACTCGGCCTC ACTCAGTTTC 
CAGCCCAAAA ACAGTGCCAC TGAGGAAGTG GAGGCATCTG GAGCAGCCGG TCAATCATCC 
ACTAGCAAGA ATGAAGAACT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msrb3 ( NM_001002094.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002094.2 Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa09240
Clone ID Related Accession (Same CDS sequence)	XM_021474986.1 , NM_001002094.2
Accession Version	NM_001002094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	methionine-R-sulfoxide reductase B3 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX119963.5. On or before Feb 23, 2016 this sequence version replaced XM_005164621.2, NM_001002094.1. Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and 5' coding region compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data because no single transcript from the reference genome strain was available for the full length of the gene. The extent of this transcript is supported by partial transcript alignments and orthologous alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01026989.1, BC071530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476800 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCCGGCT TCAATCTTCT GCATCTGGTC AGCCAAGGCC AACCTGTAAA ACTCAAGGCC 
TGCGGGCTCC CCTCAGGAAC GTGCCGAAGT AAGAAGACAT GGCCCAAAAC GTTCCCCGAG 
GAGGAGCTGA GGAAGCGTTT GACCCCAATG CAGTACCATG TTACTCAGCA GAAGGGCACA 
GAGAGCGCAT TCACAGGAAA ATATGTCGAT AATAAAGAGG AGGGAACTTA CCACTGTGTT 
GTTTGCGGTG CACCTCTTTT CATGTCTGAA AAGAAGTTTG ACTCAGGATC AGGATGGCCT 
TCTTTTTTCG ATTTGATCAA TAAGGACGCT ATAGCTCAAA CAGATGACTT TTCATACGGA 
ATGCACAGAG TGGAGACAAC CTGCAGTCAG TGTGGTGCTC ACCTGGGCCA CCTCTTCGAT 
GATGGACCTC GACCAACAAA GAAACGCTAC TGCATAAACT CGGCCTCACT CAGTTTCCAG 
CCCAAAAACA GTGCCACTGA GGAAGTGGAG GCATCTGGAG CAGCCGGTCA ATCATCCACT 
AGCAAGAATG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002094.2
CDS	210..770
Translation	MSGFNLLHLVSQGQPVKLKACGLPSGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVEASGAAGQSSTSKNEEL

Target ORF information:

RefSeq Version	NM_001002094.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002094.2

ORF Insert Sequence:

ATGTCCGGCT TCAATCTTCT GCATCTGGTC AGCCAAGGCC AACCTGTAAA ACTCAAGGCC 
TGCGGGCTCC CCTCAGGAAC GTGCCGAAGT AAGAAGACAT GGCCCAAAAC GTTCCCCGAG 
GAGGAGCTGA GGAAGCGTTT GACCCCAATG CAGTACCATG TTACTCAGCA GAAGGGCACA 
GAGAGCGCAT TCACAGGAAA ATATGTCGAT AATAAAGAGG AGGGAACTTA CCACTGTGTT 
GTTTGCGGTG CACCTCTTTT CATGTCTGAA AAGAAGTTTG ACTCAGGATC AGGATGGCCT 
TCTTTTTTCG ATTTGATCAA TAAGGACGCT ATAGCTCAAA CAGATGACTT TTCATACGGA 
ATGCACAGAG TGGAGACAAC CTGCAGTCAG TGTGGTGCTC ACCTGGGCCA CCTCTTCGAT 
GATGGACCTC GACCAACAAA GAAACGCTAC TGCATAAACT CGGCCTCACT CAGTTTCCAG 
CCCAAAAACA GTGCCACTGA GGAAGTGGAG GCATCTGGAG CAGCCGGTCA ATCATCCACT 
AGCAAGAATG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msrb3 ( XM_009300117.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298392.1 Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09238
Clone ID Related Accession (Same CDS sequence)	XM_009300117.2 , XM_009300117.1 , NM_001320378.1
Accession Version	XM_009300117.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methionine-R-sulfoxide reductase B3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300117.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCC GTCTTCTCCG ATCCATGGCC CCGTGTGCAT CCATGGCCCT GCTCCAATGC 
ATGCGCTCCC ATAAGACCAC ACGACCACTC CTAATACTGA CTACACCCGC TGCGCTAGGA 
ACGTGCCGAA GTAAGAAGAC ATGGCCCAAA ACGTTCCCCG AGGAGGAGCT GAGGAAGCGT 
TTGACCCCAA TGCAGTACCA TGTTACTCAG CAGAAGGGCA CAGAGAGCGC ATTCACAGGA 
AAATATGTCG ATAATAAAGA GGAGGGAACT TACCACTGTG TTGTTTGCGG TGCACCTCTT 
TTCATGTCTG AAAAGAAGTT TGACTCAGGA TCAGGATGGC CTTCTTTTTT CGATTTGATC 
AATAAGGACG CTATAGCTCA AACAGATGAC TTTTCATACG GAATGCACAG AGTGGAGACA 
ACCTGCAGTC AGTGTGGTGC TCACCTGGGC CACCTCTTCG ATGATGGACC TCGACCAACA 
AAGAAACGCT ACTGCATAAA CTCGGCCTCA CTCAGTTTCC AGCCCAAAAA CAGTGCCACT 
GAGGAAGTGG AGGCATCTGG AGCAGCCGGT CAATCATCCA CTAGCAAGAA TGAAGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298392.1
CDS	125..727
Translation	MAVRLLRSMAPCASMALLQCMRSHKTTRPLLILTTPAALGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVEASGAAGQSSTSKNEEL

Target ORF information:

RefSeq Version	XM_009300117.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300117.2

ORF Insert Sequence:

ATGGCTGTCC GTCTTCTCCG ATCCATGGCC CCGTGTGCAT CCATGGCCCT GCTCCAATGC 
ATGCGCTCCC ATAAGACCAC ACGACCACTC CTAATACTGA CTACACCCGC TGCGCTAGGA 
ACGTGCCGAA GTAAGAAGAC ATGGCCCAAA ACGTTCCCCG AGGAGGAGCT GAGGAAGCGT 
TTGACCCCAA TGCAGTACCA TGTTACTCAG CAGAAGGGCA CAGAGAGCGC ATTCACAGGA 
AAATATGTCG ATAATAAAGA GGAGGGAACT TACCACTGTG TTGTTTGCGG TGCACCTCTT 
TTCATGTCTG AAAAGAAGTT TGACTCAGGA TCAGGATGGC CTTCTTTTTT CGATTTGATC 
AATAAGGACG CTATAGCTCA AACAGATGAC TTTTCATACG GAATGCACAG AGTGGAGACA 
ACCTGCAGTC AGTGTGGTGC TCACCTGGGC CACCTCTTCG ATGATGGACC TCGACCAACA 
AAGAAACGCT ACTGCATAAA CTCGGCCTCA CTCAGTTTCC AGCCCAAAAA CAGTGCCACT 
GAGGAAGTGG AGGCATCTGG AGCAGCCGGT CAATCATCCA CTAGCAAGAA TGAAGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msrb3 ( XM_009300117.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298392.1 Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09238
Clone ID Related Accession (Same CDS sequence)	XM_009300117.2 , XM_009300117.1 , NM_001320378.1
Accession Version	XM_009300117.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	methionine-R-sulfoxide reductase B3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTCC GTCTTCTCCG ATCCATGGCC CCGTGTGCAT CCATGGCCCT GCTCCAATGC 
ATGCGCTCCC ATAAGACCAC ACGACCACTC CTAATACTGA CTACACCCGC TGCGCTAGGA 
ACGTGCCGAA GTAAGAAGAC ATGGCCCAAA ACGTTCCCCG AGGAGGAGCT GAGGAAGCGT 
TTGACCCCAA TGCAGTACCA TGTTACTCAG CAGAAGGGCA CAGAGAGCGC ATTCACAGGA 
AAATATGTCG ATAATAAAGA GGAGGGAACT TACCACTGTG TTGTTTGCGG TGCACCTCTT 
TTCATGTCTG AAAAGAAGTT TGACTCAGGA TCAGGATGGC CTTCTTTTTT CGATTTGATC 
AATAAGGACG CTATAGCTCA AACAGATGAC TTTTCATACG GAATGCACAG AGTGGAGACA 
ACCTGCAGTC AGTGTGGTGC TCACCTGGGC CACCTCTTCG ATGATGGACC TCGACCAACA 
AAGAAACGCT ACTGCATAAA CTCGGCCTCA CTCAGTTTCC AGCCCAAAAA CAGTGCCACT 
GAGGAAGTGG AGGCATCTGG AGCAGCCGGT CAATCATCCA CTAGCAAGAA TGAAGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298392.1
CDS	110..712
Translation	MAVRLLRSMAPCASMALLQCMRSHKTTRPLLILTTPAALGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVEASGAAGQSSTSKNEEL

Target ORF information:

RefSeq Version	XM_009300117.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300117.1

ORF Insert Sequence:

ATGGCTGTCC GTCTTCTCCG ATCCATGGCC CCGTGTGCAT CCATGGCCCT GCTCCAATGC 
ATGCGCTCCC ATAAGACCAC ACGACCACTC CTAATACTGA CTACACCCGC TGCGCTAGGA 
ACGTGCCGAA GTAAGAAGAC ATGGCCCAAA ACGTTCCCCG AGGAGGAGCT GAGGAAGCGT 
TTGACCCCAA TGCAGTACCA TGTTACTCAG CAGAAGGGCA CAGAGAGCGC ATTCACAGGA 
AAATATGTCG ATAATAAAGA GGAGGGAACT TACCACTGTG TTGTTTGCGG TGCACCTCTT 
TTCATGTCTG AAAAGAAGTT TGACTCAGGA TCAGGATGGC CTTCTTTTTT CGATTTGATC 
AATAAGGACG CTATAGCTCA AACAGATGAC TTTTCATACG GAATGCACAG AGTGGAGACA 
ACCTGCAGTC AGTGTGGTGC TCACCTGGGC CACCTCTTCG ATGATGGACC TCGACCAACA 
AAGAAACGCT ACTGCATAAA CTCGGCCTCA CTCAGTTTCC AGCCCAAAAA CAGTGCCACT 
GAGGAAGTGG AGGCATCTGG AGCAGCCGGT CAATCATCCA CTAGCAAGAA TGAAGAACTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msrb3 ( XM_021474986.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330661.1 Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X2, mRNA.

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CloneID	ODa09240
Clone ID Related Accession (Same CDS sequence)	XM_021474986.1 , NM_001002094.2
Accession Version	XM_021474986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	methionine-R-sulfoxide reductase B3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGCT TCAATCTTCT GCATCTGGTC AGCCAAGGCC AACCTGTAAA ACTCAAGGCC 
TGCGGGCTCC CCTCAGGAAC GTGCCGAAGT AAGAAGACAT GGCCCAAAAC GTTCCCCGAG 
GAGGAGCTGA GGAAGCGTTT GACCCCAATG CAGTACCATG TTACTCAGCA GAAGGGCACA 
GAGAGCGCAT TCACAGGAAA ATATGTCGAT AATAAAGAGG AGGGAACTTA CCACTGTGTT 
GTTTGCGGTG CACCTCTTTT CATGTCTGAA AAGAAGTTTG ACTCAGGATC AGGATGGCCT 
TCTTTTTTCG ATTTGATCAA TAAGGACGCT ATAGCTCAAA CAGATGACTT TTCATACGGA 
ATGCACAGAG TGGAGACAAC CTGCAGTCAG TGTGGTGCTC ACCTGGGCCA CCTCTTCGAT 
GATGGACCTC GACCAACAAA GAAACGCTAC TGCATAAACT CGGCCTCACT CAGTTTCCAG 
CCCAAAAACA GTGCCACTGA GGAAGTGGAG GCATCTGGAG CAGCCGGTCA ATCATCCACT 
AGCAAGAATG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330661.1
CDS	375..935
Translation	MSGFNLLHLVSQGQPVKLKACGLPSGTCRSKKTWPKTFPEEELRKRLTPMQYHVTQQKGTESAFTGKYVDNKEEGTYHCVVCGAPLFMSEKKFDSGSGWPSFFDLINKDAIAQTDDFSYGMHRVETTCSQCGAHLGHLFDDGPRPTKKRYCINSASLSFQPKNSATEEVEASGAAGQSSTSKNEEL

Target ORF information:

RefSeq Version	XM_021474986.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B3 (msrb3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474986.1

ORF Insert Sequence:

ATGTCCGGCT TCAATCTTCT GCATCTGGTC AGCCAAGGCC AACCTGTAAA ACTCAAGGCC 
TGCGGGCTCC CCTCAGGAAC GTGCCGAAGT AAGAAGACAT GGCCCAAAAC GTTCCCCGAG 
GAGGAGCTGA GGAAGCGTTT GACCCCAATG CAGTACCATG TTACTCAGCA GAAGGGCACA 
GAGAGCGCAT TCACAGGAAA ATATGTCGAT AATAAAGAGG AGGGAACTTA CCACTGTGTT 
GTTTGCGGTG CACCTCTTTT CATGTCTGAA AAGAAGTTTG ACTCAGGATC AGGATGGCCT 
TCTTTTTTCG ATTTGATCAA TAAGGACGCT ATAGCTCAAA CAGATGACTT TTCATACGGA 
ATGCACAGAG TGGAGACAAC CTGCAGTCAG TGTGGTGCTC ACCTGGGCCA CCTCTTCGAT 
GATGGACCTC GACCAACAAA GAAACGCTAC TGCATAAACT CGGCCTCACT CAGTTTCCAG 
CCCAAAAACA GTGCCACTGA GGAAGTGGAG GCATCTGGAG CAGCCGGTCA ATCATCCACT 
AGCAAGAATG AAGAACTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

Novel Genes Critical for Hypoxic Preconditioning in Zebrafish Are Regulators of Insulin and Glucose Metabolism.
G3 (Bethesda, Md.)5(6)1107-16(2015 Apr)
Manchenkov T,Pasillas MP,Haddad GG,Imam FB

Down-regulation of msrb3 and destruction of normal auditory system development through hair cell apoptosis in zebrafish.
The International journal of developmental biology59(4-6)195-203(2015)
Shen X,Liu F,Wang Y,Wang H,Ma J,Xia W,Zhang J,Jiang N,Sun S,Wang X,Ma D

Porcine methionine sulfoxide reductase B3: molecular cloning, tissue-specific expression profiles, and polymorphisms associated with ear size in Sus scrofa.
Journal of animal science and biotechnology660(2015)
Zhang Y,Liang J,Zhang L,Wang L,Liu X,Yan H,Zhao K,Shi H,Zhang T,Li N,Pu L,Wang L

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msrb3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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