TMEM8C cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM8C
Entrez Gene ID	415178
Full Name	transmembrane protein 8C
Synonyms	mymk,si:dkey-191h14.1,wu:fb30h02,zgc:86927
General protein information	Preferred Names transmembrane protein 8C Names protein myomaker fb30h02 myoblast fusion maker myomaker
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356053.2	XP_017211542.1	protein myomaker isoform X1
XM_017356054.1	XP_017211543.1	protein myomaker isoform X2
NM_001002088.1	NP_001002088.1	protein myomaker
XM_017356053.1	XP_017211542.1	protein myomaker isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TMEM8C	XP_003639355.1
Gallus gallus (chicken)	TMEM8C	XP_001236919.2
Mus musculus (house mouse)	Tmem8c	NP_079652.1
Homo sapiens (human)	TMEM8C	NP_001073952.1
Bos taurus (cattle)	TMEM8C	NP_001179975.1
Danio rerio (zebrafish)	tmem8c	NP_001002088.1

Gene Ontology
Bibliography

Related articles in PubMed

Requirement of the fusogenic micropeptide myomixer for muscle formation in zebrafish.
Shi J, Bi P, Pei J, Li H, Grishin NV, Bassel-Duby R, Chen EH, Olson EN
Proceedings of the National Academy of Sciences of the United States of America114(45)11950-11955(2017 Nov)

A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
Di Gioia SA, Connors S, Matsunami N, Cannavino J, Rose MF, Gilette NM, Artoni P, de Macena Sobreira NL, Chan WM, Webb BD, Robson CD, Cheng L, Van Ryzin C, Ramirez-Martinez A, Mohassel P, Leppert M, Scholand MB, Grunseich C, Ferreira CR, Hartman T, Hayes IM, Morgan T, Markie DM, Fagiolini M, Swift A, Chines PS, Speck-Martins CE, Collins FS, Jabs EW, B?nnemann CG, Olson EN, , Carey JC, Robertson SP, Manoli I, Engle EC
Nature communications816077(2017 Jul)

Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo.
Zhang W, Roy S
Developmental biology423(1)24-33(2017 03)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings underscore a conserved role for Myomaker in vertebrate myocyte fusion.
Title: Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo.

Confocal microscopy showed marked phenotype characterized by persistence of mononucleated muscle cells in fast myotome at developmental stages where these cells normally fuse to form multinucleated myotubes. myomaker is essential for myocyte fusion.
Title: Myomaker mediates fusion of fast myocytes in zebrafish embryos.

The following TMEM8C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM8C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38465	NM_001002088.1 Latest version!	Danio rerio myomaker, myoblast fusion factor (mymk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa59064	XM_017356053.2 Latest version!	Danio rerio transmembrane protein 8C (tmem8c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
ODa59065	XM_017356054.1 Latest version!	Danio rerio transmembrane protein 8C (tmem8c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
ODa59064	XM_017356053.1	Danio rerio transmembrane protein 8C (tmem8c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM8C ( NM_001002088.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001002088.1 Danio rerio myomaker, myoblast fusion factor (mymk), mRNA.

Price & Availability↑

CloneID	ODa38465
Clone ID Related Accession (Same CDS sequence)	NM_001002088.1
Accession Version	NM_001002088.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Danio rerio(zebrafish)
Product	protein myomaker
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071541.1. ##Evidence-Data-START## Transcript exon combination :: BC071541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476732, SAMEA4476738 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATTTACCAG GACCGACTGG GCTACGGCAT TTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002088.1
CDS	124..786
Translation	MGAFIAKMLLPTISSLVFVPAASVAAKRGFHMEAMVYFFTMFFTAIYHACDGPGLSILCFMKYDILEYFSVYGTAISMWVTLLALGDFDEPKRSSLTMFGVLTAAVRIYQDRLGYGIYSGPIGTAVFMITVKWLQKMKEKKGLYPDKSVYTQQVGPGCCFGALALMLRFYFEEWDYAYVHSFYHVSLAMSFILLLPKKNRYAGTGRNAAKLNCYTLCCCV

Target ORF information:

RefSeq Version	NM_001002088.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myomaker, myoblast fusion factor (mymk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002088.1

ORF Insert Sequence:

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATTTACCAG GACCGACTGG GCTACGGCAT TTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM8C ( XM_017356053.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211542.1 Danio rerio transmembrane protein 8C (tmem8c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59064
Clone ID Related Accession (Same CDS sequence)	XM_017356053.2 , XM_017356053.1
Accession Version	XM_017356053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein myomaker isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATCTACCAG GACCGACTGG GATACGGCAT CTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTACT 
TCACAACCTC AGATGAAGGA AAAAGCACGG ACTATCTGGA CTACTCCGCA GAAACCCTGG 
ATGCGGACCT GTGGTCCTGG CCTGCCGTTA CACAAATCCC GGCCACGCAC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211542.1
CDS	205..981
Translation	MGAFIAKMLLPTISSLVFVPAASVAAKRGFHMEAMVYFFTMFFTAIYHACDGPGLSILCFMKYDILEYFSVYGTAISMWVTLLALGDFDEPKRSSLTMFGVLTAAVRIYQDRLGYGIYSGPIGTAVFMITVKWLQKMKEKKGLYPDKSVYTQQVGPGCCFGALALMLRFYFEEWDYAYVHSFYHVSLAMSFILLLPKKNRYAGTGRNAAKLNCYTLCCCTSQPQMKEKARTIWTTPQKPWMRTCGPGLPLHKSRPRTM

Target ORF information:

RefSeq Version	XM_017356053.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane protein 8C (tmem8c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356053.2

ORF Insert Sequence:

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATCTACCAG GACCGACTGG GATACGGCAT CTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTACT 
TCACAACCTC AGATGAAGGA AAAAGCACGG ACTATCTGGA CTACTCCGCA GAAACCCTGG 
ATGCGGACCT GTGGTCCTGG CCTGCCGTTA CACAAATCCC GGCCACGCAC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM8C ( XM_017356053.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211542.1 Danio rerio transmembrane protein 8C (tmem8c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa59064
Clone ID Related Accession (Same CDS sequence)	XM_017356053.2 , XM_017356053.1
Accession Version	XM_017356053.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein myomaker isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATCTACCAG GACCGACTGG GATACGGCAT CTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTACT 
TCACAACCTC AGATGAAGGA AAAAGCACGG ACTATCTGGA CTACTCCGCA GAAACCCTGG 
ATGCGGACCT GTGGTCCTGG CCTGCCGTTA CACAAATCCC GGCCACGCAC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211542.1
CDS	188..964
Translation	MGAFIAKMLLPTISSLVFVPAASVAAKRGFHMEAMVYFFTMFFTAIYHACDGPGLSILCFMKYDILEYFSVYGTAISMWVTLLALGDFDEPKRSSLTMFGVLTAAVRIYQDRLGYGIYSGPIGTAVFMITVKWLQKMKEKKGLYPDKSVYTQQVGPGCCFGALALMLRFYFEEWDYAYVHSFYHVSLAMSFILLLPKKNRYAGTGRNAAKLNCYTLCCCTSQPQMKEKARTIWTTPQKPWMRTCGPGLPLHKSRPRTM

Target ORF information:

RefSeq Version	XM_017356053.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 8C (tmem8c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356053.1

ORF Insert Sequence:

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATCTACCAG GACCGACTGG GATACGGCAT CTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTACT 
TCACAACCTC AGATGAAGGA AAAAGCACGG ACTATCTGGA CTACTCCGCA GAAACCCTGG 
ATGCGGACCT GTGGTCCTGG CCTGCCGTTA CACAAATCCC GGCCACGCAC CATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM8C ( XM_017356054.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017211543.1 Danio rerio transmembrane protein 8C (tmem8c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa59065
Clone ID Related Accession (Same CDS sequence)	XM_017356054.1
Accession Version	XM_017356054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein myomaker isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATCTACCAG GACCGACTGG GATACGGCAT CTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211543.1
CDS	188..850
Translation	MGAFIAKMLLPTISSLVFVPAASVAAKRGFHMEAMVYFFTMFFTAIYHACDGPGLSILCFMKYDILEYFSVYGTAISMWVTLLALGDFDEPKRSSLTMFGVLTAAVRIYQDRLGYGIYSGPIGTAVFMITVKWLQKMKEKKGLYPDKSVYTQQVGPGCCFGALALMLRFYFEEWDYAYVHSFYHVSLAMSFILLLPKKNRYAGTGRNAAKLNCYTLCCCV

Target ORF information:

RefSeq Version	XM_017356054.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transmembrane protein 8C (tmem8c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356054.1

ORF Insert Sequence:

ATGGGAGCGT TTATCGCCAA GATGTTGCTG CCCACTATTA GCAGTTTGGT GTTTGTGCCT 
GCAGCCAGCG TGGCTGCAAA GAGGGGCTTC CACATGGAGG CCATGGTCTA TTTCTTCACA 
ATGTTCTTCA CCGCGATTTA CCACGCATGT GACGGTCCGG GCTTGTCCAT TCTCTGTTTC 
ATGAAGTATG ACATTCTGGA GTACTTCAGC GTGTACGGGA CAGCCATCTC CATGTGGGTC 
ACGCTACTGG CGCTTGGGGA TTTCGATGAG CCCAAACGCT CTTCGCTCAC CATGTTTGGG 
GTGTTGACCG CAGCTGTGAG GATCTACCAG GACCGACTGG GATACGGCAT CTACTCCGGC 
CCCATCGGGA CAGCTGTCTT TATGATCACA GTCAAATGGT TACAGAAAAT GAAGGAAAAG 
AAAGGCCTTT ATCCAGACAA AAGTGTTTAC ACTCAACAAG TGGGCCCAGG GTGCTGCTTC 
GGTGCTCTTG CTTTGATGCT TCGCTTCTAT TTTGAGGAGT GGGACTACGC TTATGTTCAC 
AGTTTCTACC ACGTGTCTCT GGCCATGTCC TTTATTCTGC TGCTGCCCAA GAAGAACCGT 
TATGCTGGAA CGGGACGTAA CGCAGCCAAA CTCAACTGCT ACACCCTCTG CTGCTGTGTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Requirement of the fusogenic micropeptide myomixer for muscle formation in zebrafish.
Proceedings of the National Academy of Sciences of the United States of America114(45)11950-11955(2017 Nov)
Shi J,Bi P,Pei J,Li H,Grishin NV,Bassel-Duby R,Chen EH,Olson EN

A defect in myoblast fusion underlies Carey-Fineman-Ziter syndrome.
Nature communications816077(2017 Jul)
Di Gioia SA,Connors S,Matsunami N,Cannavino J,Rose MF,Gilette NM,Artoni P,de Macena Sobreira NL,Chan WM,Webb BD,Robson CD,Cheng L,Van Ryzin C,Ramirez-Martinez A,Mohassel P,Leppert M,Scholand MB,Grunseich C,Ferreira CR,Hartman T,Hayes IM,Morgan T,Markie DM,Fagiolini M,Swift A,Chines PS,Speck-Martins CE,Collins FS,Jabs EW,B?nnemann CG,Olson EN,,Carey JC,Robertson SP,Manoli I,Engle EC

Myomaker is required for the fusion of fast-twitch myocytes in the zebrafish embryo.
Developmental biology423(1)24-33(2017 03)
Zhang W,Roy S

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TMEM8C cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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