npepps cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npepps
Entrez Gene ID	407726
Full Name	aminopeptidase puromycin sensitive
Synonyms	Psa,fb68d07,psa,sb:cb848,wu:fb68d07
General protein information	Preferred Names aminopeptidase puromycin sensitive Names puromycin-sensitive aminopeptidase
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164027.1	NP_001157499.1	puromycin-sensitive aminopeptidase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Psa	NP_728614.1
Arabidopsis thaliana (thale cress)	APM1	NP_195035.2
	NPEPPS	XP_001082709.2
Rattus norvegicus (Norway rat)	Npepps	NP_536320.1
Gallus gallus (chicken)	NPEPPS	XP_001234986.3
Caenorhabditis elegans (roundworm)	pam-1	NP_001023210.1
Saccharomyces cerevisiae (baker's yeast)	APE2	NP_012765.3
Schizosaccharomyces pombe (fission yeast)	ape2	NP_595998.1
Oryza sativa (rice)	Os02g0218200	NP_001046303.1
Homo sapiens (human)	NPEPPS	NP_006301.3
Canis lupus familiaris (dog)	NPEPPS	XP_537659.4
Mus musculus (house mouse)	Npepps	NP_032968.2
Eremothecium gossypii	AGOS_AAL127W	NP_982415.2
Xenopus tropicalis (tropical clawed frog)	npepps	NP_001072690.1
Pan troglodytes (chimpanzee)	NPEPPS	XP_001173517.2
Bos taurus (cattle)	NPEPPS	NP_001180088.1
Neurospora crassa	NCU09228	XP_959172.1
Danio rerio (zebrafish)	npepps	NP_001157499.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005728	XP_556379.3
Kluyveromyces lactis	KLLA0C11033g	XP_452692.2
Magnaporthe oryzae (rice blast fungus)	MGG_16472	XP_003710702.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Dynamics of zebrafish fin regeneration using a pulsed SILAC approach.
Nolte H, H?lper S, Housley MP, Islam S, Piller T, Konzer A, Stainier DY, Braun T, Kr?ger M
Proteomics15(4)739-51(2015 Feb)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

Identification of a puromycin-sensitive aminopeptidase from zebrafish (Danio rerio).
Chen SH, Cao MJ, Huang JZ, Wu GP
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology159(1)10-7(2011 May)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the purification and characterization of a puromycin-sensitive aminopeptidase from zebrafish
Title: Identification of a puromycin-sensitive aminopeptidase from zebrafish (Danio rerio).

The following npepps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npepps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00621	NM_001164027.1 Latest version!	Danio rerio aminopeptidase puromycin sensitive (npepps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npepps ( NM_001164027.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001157499.1 Danio rerio aminopeptidase puromycin sensitive (npepps), mRNA.

Price & Availability↑

CloneID	ODa00621
Clone ID Related Accession (Same CDS sequence)	NM_001164027.1
Accession Version	NM_001164027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	puromycin-sensitive aminopeptidase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX469912.6. On Aug 10, 2009 this sequence version replaced XM_678950.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: lack of evidence for use of upstream AUG ##RefSeq-Attributes-END##

ATGCCCGAGC GGAGGCCCTT CGTGCGGTTG CCCACTGACG TTTACCCCGT CAACTACGGG 
CTGCGCCTCA AGCCCGACCT CGTCGACTTC ACCTTCGAGG GCAAACTGGA GGCAGCCGTG 
GAGGTTACAC AAGGGACAAA CCAGATTGTG ATGAACTGTG CTGATATAGA CATAATTACT 
GCATCTTTTG TGCCTGACGG GGGGGAAGAA ATTAACGCTA CGGGATTTAA CTATCAGAAC 
GAGGATGAGA AGGTGACCCT ATGTTTTCCC AGCACTCTGC AAAAAGGTTC GGGATCATTG 
AAAATAGACT TTGTTGGAGA GCTGAATGAC AAAATGAAAG GCTTCTATAG GAGTAAATAC 
TCTTCTTCGG GTGAAGTCCG CTATGCTGCC GTCACACAGT TTGAGGCCAC CGATGCACGT 
CGAGCTTTCC CTTGCTGGGA TGAACCAGCT ATCAAAGCCA CTTTTGACAT CACCCTCATC 
GTCCCAAAGG ACAGAGTAGC CTTGTCGAAT ATGAATGTTG TTGACCGAAA GCCATATGCA 
GAGGATCAGA GTCTGGTGGA GGTGAAGTTT GCCACAACAC CCATCATGTC CACCTATCTA 
GTGGCATTTG TCATTGGCGA GTATGACTTT GTGGAGAGCC AGTCGTCAGA TGGTGTGACA 
GTCCGTGTGT ATACTCCTGT TGGAAAGGCA GAGCAAGGGA AATTTGCACT AGAGGTTGCC 
ACAAAGACCC TTCCGTTCTA CAAAGATTAT TTCAGTGTTC CTTATCCGTT GCCTAAAATT 
GATCTAATTG CTATTGCTGA TTTTGCTGCT GGTGCCATGG AAAACTGGGG CCTTGTTACT 
TACAGAGAGA CAGCGCTGCT GATTGACCCA AAGAATTCGT GTGCATCGTC CCGCCAATGG 
GTCGCTCTTG TAGTCGGTCA TGAACTGGCA CATCAGTGGT TCGGGAATCT AGTTACCATG 
GAATGGTGGA CCCATTTATG GCTTAATGAA GGCTTTGCAT CTTGGATCGA GTACCTCTGT 
GTGGACCACT GTTTTCCCGA ATACGACATC TGGACCCAGT TTGTATCTGC TGATTACACC 
AGGGCTCTGG ACCTGGATGC GCTAGATAAC AGCCACCCTA TTGAGGTTGA TGTCGGACAC 
CCTTCTGAAG TGGATGAAAT ATTCGATGCC ATATCATACA GCAAAGGGGC CTCTGTGATT 
CGCATGCTGC ATAACTACAT TGGCGATGAG GATTTTAGAA AGGGAATGAA TGCTTACCTT 
TTGAAGTTTC AGCACAAAAA TGCATCTACT GAGGACCTAT GGGAATGTTT GGAGCAAGCA 
AGTGGGAAGC CTATTGCTGC AGTAATGAAT TCCTGGACCA AACAAATGGG CTTCCCTATT 
ATTGTAGTGG ATCAAGAGCA GCATGGCAGT GACCGTGTGC TGAAAATCTC TCAGAAGAAG 
TTCTGTGCTA GCGGACCACG TAATGATGAG GACTGCCCCA ACTGGATGGT TCCCATTAGC 
ATCTGCACCA GTGAGGACCC GAGCTGCACT AAGACAAAGA TTCTGCTGGA CCAGCCTGAA 
ACCACAGTTA ACATTACCAA TGTTGCACCT GACCATTGGA TCAAGATTAA TCCAGGAACT 
GTGGGATTCT ACAGGATTCA GTACAGCTCC GCCATGCTGG AGAGTTTGCT GCCGGGTATC 
AGGGACCTCA CACTGCTGCC TGTGGACCGT CTGGGTCTGC AGAACGACCT CTTCTCCTTG 
GCTCGAGCAG GTATGATCAG TACTGTGGAG GTGTTGAAGG TGATGGAAGC ATTTGTGAAT 
GAGCCAAACT ACACGGTGTG GAGTGACCTG AGCTGTAATC TGGGTGTTCT GTCCTCTCTG 
CTCTCACACA CTGACTTCCA CGAGGACATC CAGGAGTTCA TCCGAGATCT CTTCACCCCC 
ATCGGCATGA AGCTAGGATG GGACAGCAGA ACCGGAGAGG GTCACCTGGA TGCCCTGTTG 
CGGGGTCTGG TGCTGGGGAA GCTGGGTAAA GCGGGGCACA AGGCCACTCT GGAGGAGGCC 
CGGCGGAGAT TCAGAGAGCA TGTGGAGGGT AAACAGATCC TGTCTGCAGA CCTCAGGAGT 
CCAGTGTATC TAACAGTTTT GAAGCACGGA GACAGCACTA CACTTGACAC CATGCTGAAG 
CTTCATAAAC AGGCAGACAT GCAGGAGGAG AAGAACCGCA TCGAGAGAGT GCTGGGAGCC 
ATTCCTGCCC CTGACCTCAT CCAGAGGGTT CTTAACTTTG CCCTGTCGGA GGAGGTTCGT 
CCTCAGGACA CTGTTTCTGT AATCGGGGGT GTTGCTGGGA GCAGCAAACA GGGCCGCAAA 
GCCGCGTGGA AATTTGTAAA GGACAACTGG GAAGAACTTC ACAACCGTTA TCAGGGTGGC 
TTCCTCATAT CAAGACTCAT CAAGCTGACG GTGGATGGGT TTGCAATAGA TAAAATGGCT 
GCTGAAGTTA AGAGTTTCTT TGAAAGTCAT CATGCGCCAG CGGCCGAGCG CACGGTACAG 
CAGTGCTGCG AGAACATCCT CCTGAACGCC GCCTGGCTGA AAAGAGACGC AGACGACATC 
CACCAGTATC TGCTTCAGCG GAAAACACCA CCCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157499.1
CDS	149..2767
Translation	MPERRPFVRLPTDVYPVNYGLRLKPDLVDFTFEGKLEAAVEVTQGTNQIVMNCADIDIITASFVPDGGEEINATGFNYQNEDEKVTLCFPSTLQKGSGSLKIDFVGELNDKMKGFYRSKYSSSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDITLIVPKDRVALSNMNVVDRKPYAEDQSLVEVKFATTPIMSTYLVAFVIGEYDFVESQSSDGVTVRVYTPVGKAEQGKFALEVATKTLPFYKDYFSVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCASSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRALDLDALDNSHPIEVDVGHPSEVDEIFDAISYSKGASVIRMLHNYIGDEDFRKGMNAYLLKFQHKNASTEDLWECLEQASGKPIAAVMNSWTKQMGFPIIVVDQEQHGSDRVLKISQKKFCASGPRNDEDCPNWMVPISICTSEDPSCTKTKILLDQPETTVNITNVAPDHWIKINPGTVGFYRIQYSSAMLESLLPGIRDLTLLPVDRLGLQNDLFSLARAGMISTVEVLKVMEAFVNEPNYTVWSDLSCNLGVLSSLLSHTDFHEDIQEFIRDLFTPIGMKLGWDSRTGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFREHVEGKQILSADLRSPVYLTVLKHGDSTTLDTMLKLHKQADMQEEKNRIERVLGAIPAPDLIQRVLNFALSEEVRPQDTVSVIGGVAGSSKQGRKAAWKFVKDNWEELHNRYQGGFLISRLIKLTVDGFAIDKMAAEVKSFFESHHAPAAERTVQQCCENILLNAAWLKRDADDIHQYLLQRKTPPV

Target ORF information:

RefSeq Version	NM_001164027.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio aminopeptidase puromycin sensitive (npepps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164027.1

ORF Insert Sequence:

ATGCCCGAGC GGAGGCCCTT CGTGCGGTTG CCCACTGACG TTTACCCCGT CAACTACGGG 
CTGCGCCTCA AGCCCGACCT CGTCGACTTC ACCTTCGAGG GCAAACTGGA GGCAGCCGTG 
GAGGTTACAC AAGGGACAAA CCAGATTGTG ATGAACTGTG CTGATATAGA CATAATTACT 
GCATCTTTTG TGCCTGACGG GGGGGAAGAA ATTAACGCTA CGGGATTTAA CTATCAGAAC 
GAGGATGAGA AGGTGACCCT ATGTTTTCCC AGCACTCTGC AAAAAGGTTC GGGATCATTG 
AAAATAGACT TTGTTGGAGA GCTGAATGAC AAAATGAAAG GCTTCTATAG GAGTAAATAC 
TCTTCTTCGG GTGAAGTCCG CTATGCTGCC GTCACACAGT TTGAGGCCAC CGATGCACGT 
CGAGCTTTCC CTTGCTGGGA TGAACCAGCT ATCAAAGCCA CTTTTGACAT CACCCTCATC 
GTCCCAAAGG ACAGAGTAGC CTTGTCGAAT ATGAATGTTG TTGACCGAAA GCCATATGCA 
GAGGATCAGA GTCTGGTGGA GGTGAAGTTT GCCACAACAC CCATCATGTC CACCTATCTA 
GTGGCATTTG TCATTGGCGA GTATGACTTT GTGGAGAGCC AGTCGTCAGA TGGTGTGACA 
GTCCGTGTGT ATACTCCTGT TGGAAAGGCA GAGCAAGGGA AATTTGCACT AGAGGTTGCC 
ACAAAGACCC TTCCGTTCTA CAAAGATTAT TTCAGTGTTC CTTATCCGTT GCCTAAAATT 
GATCTAATTG CTATTGCTGA TTTTGCTGCT GGTGCCATGG AAAACTGGGG CCTTGTTACT 
TACAGAGAGA CAGCGCTGCT GATTGACCCA AAGAATTCGT GTGCATCGTC CCGCCAATGG 
GTCGCTCTTG TAGTCGGTCA TGAACTGGCA CATCAGTGGT TCGGGAATCT AGTTACCATG 
GAATGGTGGA CCCATTTATG GCTTAATGAA GGCTTTGCAT CTTGGATCGA GTACCTCTGT 
GTGGACCACT GTTTTCCCGA ATACGACATC TGGACCCAGT TTGTATCTGC TGATTACACC 
AGGGCTCTGG ACCTGGATGC GCTAGATAAC AGCCACCCTA TTGAGGTTGA TGTCGGACAC 
CCTTCTGAAG TGGATGAAAT ATTCGATGCC ATATCATACA GCAAAGGGGC CTCTGTGATT 
CGCATGCTGC ATAACTACAT TGGCGATGAG GATTTTAGAA AGGGAATGAA TGCTTACCTT 
TTGAAGTTTC AGCACAAAAA TGCATCTACT GAGGACCTAT GGGAATGTTT GGAGCAAGCA 
AGTGGGAAGC CTATTGCTGC AGTAATGAAT TCCTGGACCA AACAAATGGG CTTCCCTATT 
ATTGTAGTGG ATCAAGAGCA GCATGGCAGT GACCGTGTGC TGAAAATCTC TCAGAAGAAG 
TTCTGTGCTA GCGGACCACG TAATGATGAG GACTGCCCCA ACTGGATGGT TCCCATTAGC 
ATCTGCACCA GTGAGGACCC GAGCTGCACT AAGACAAAGA TTCTGCTGGA CCAGCCTGAA 
ACCACAGTTA ACATTACCAA TGTTGCACCT GACCATTGGA TCAAGATTAA TCCAGGAACT 
GTGGGATTCT ACAGGATTCA GTACAGCTCC GCCATGCTGG AGAGTTTGCT GCCGGGTATC 
AGGGACCTCA CACTGCTGCC TGTGGACCGT CTGGGTCTGC AGAACGACCT CTTCTCCTTG 
GCTCGAGCAG GTATGATCAG TACTGTGGAG GTGTTGAAGG TGATGGAAGC ATTTGTGAAT 
GAGCCAAACT ACACGGTGTG GAGTGACCTG AGCTGTAATC TGGGTGTTCT GTCCTCTCTG 
CTCTCACACA CTGACTTCCA CGAGGACATC CAGGAGTTCA TCCGAGATCT CTTCACCCCC 
ATCGGCATGA AGCTAGGATG GGACAGCAGA ACCGGAGAGG GTCACCTGGA TGCCCTGTTG 
CGGGGTCTGG TGCTGGGGAA GCTGGGTAAA GCGGGGCACA AGGCCACTCT GGAGGAGGCC 
CGGCGGAGAT TCAGAGAGCA TGTGGAGGGT AAACAGATCC TGTCTGCAGA CCTCAGGAGT 
CCAGTGTATC TAACAGTTTT GAAGCACGGA GACAGCACTA CACTTGACAC CATGCTGAAG 
CTTCATAAAC AGGCAGACAT GCAGGAGGAG AAGAACCGCA TCGAGAGAGT GCTGGGAGCC 
ATTCCTGCCC CTGACCTCAT CCAGAGGGTT CTTAACTTTG CCCTGTCGGA GGAGGTTCGT 
CCTCAGGACA CTGTTTCTGT AATCGGGGGT GTTGCTGGGA GCAGCAAACA GGGCCGCAAA 
GCCGCGTGGA AATTTGTAAA GGACAACTGG GAAGAACTTC ACAACCGTTA TCAGGGTGGC 
TTCCTCATAT CAAGACTCAT CAAGCTGACG GTGGATGGGT TTGCAATAGA TAAAATGGCT 
GCTGAAGTTA AGAGTTTCTT TGAAAGTCAT CATGCGCCAG CGGCCGAGCG CACGGTACAG 
CAGTGCTGCG AGAACATCCT CCTGAACGCC GCCTGGCTGA AAAGAGACGC AGACGACATC 
CACCAGTATC TGCTTCAGCG GAAAACACCA CCCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Dynamics of zebrafish fin regeneration using a pulsed SILAC approach.
Proteomics15(4)739-51(2015 Feb)
Nolte H,H?lper S,Housley MP,Islam S,Piller T,Konzer A,Stainier DY,Braun T,Kr?ger M

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

Identification of a puromycin-sensitive aminopeptidase from zebrafish (Danio rerio).
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology159(1)10-7(2011 May)
Chen SH,Cao MJ,Huang JZ,Wu GP

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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npepps cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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