tph2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tph2
Entrez Gene ID	407712
Full Name	tryptophan hydroxylase 2 (tryptophan 5-monooxygenase)
Synonyms	tphR,wu:fq15a04
General protein information	Preferred Names tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) Names tryptophan 5-hydroxylase 2 tph2a tryptophan hydroxylase R
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214795.2	NP_999960.2	tryptophan 5-hydroxylase 2 isoform 2
NM_001310068.1	NP_001296997.1	tryptophan 5-hydroxylase 2 isoform 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tph2	NP_776211.1
Caenorhabditis elegans (roundworm)	tph-1	NP_495584.4
Homo sapiens (human)	TPH2	NP_775489.2
Canis lupus familiaris (dog)	TPH2	NP_001184049.1
Gallus gallus (chicken)	TPH2	NP_001001301.1
Mus musculus (house mouse)	Tph2	NP_775567.2
Xenopus tropicalis (tropical clawed frog)	tph2	XP_004913008.1
Pan troglodytes (chimpanzee)	TPH2	XP_522470.2
	TPH2	NP_001035035.1
Danio rerio (zebrafish)	tph2	NP_999960.1

Gene Ontology

The following tph2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tph2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa23242	NM_001310068.1 Latest version!	Danio rerio tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) (tph2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40969	NM_214795.2 Latest version!	Danio rerio tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) (tph2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tph2 ( NM_001310068.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001296997.1 Danio rerio tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) (tph2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa23242
Clone ID Related Accession (Same CDS sequence)	NM_001310068.1
Accession Version	NM_001310068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	tryptophan 5-hydroxylase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX950194.9 and EB870154.1. On Jun 25, 2015 this sequence version replaced XM_005159075.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY616134.1, BC162621.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGGGA AGAAAACCTC TCAAGAGACA ACAGCAACTA TGTCTAATGA ACTGCAAACT 
AATCCAAAGG GGCAGCAAAC AGCCCCTAAA TCACCTGTTG GGCTTCTTAG GAAAAGAGGT 
CAAGAAGCAA TTCCGAAGAT GCAACCTGCC ATGATGATGT TCTCCAGCAA ATACTGGGCT 
CGGAGAGGAC TATCTTTGGA TTCAGCTATG TATGACCAAC AGCACCTTGC ATCCTCCATG 
CTCCGCAGGA CATCCTTTAA TCGGATAGAT GAAAGACCTG ACAAAGAAGA ACAGAAATCC 
ACTCATGACC TTGGGAAGCT GGCAGTGATT TTCTCTCTGA AGAATGAAGT TGGCTTTCTG 
GTGAAAGCGC TGAGGCTCTT TCAGGAGAAG CATGTGAATT TGGCGCACAT TGAGTCTCGG 
AGATCAAAGA GACTCACCAA TGAGATTGAG ATATATGCAG AATGCAACTG CACAAAGAAA 
GAGTTCAACG AACTGGTGCA ACACCTCAAA GACCACGTTA ATATTGTCTC GTATAACACA 
CCTCAACATG TGTGGTCTGC AGAGACCGAC TGTTTGGACT GTGTGTGTGT TTTGGGTGGA 
TTGCCAGATG GGGAAGGAAT ACCATGGTTT CCCCAAAAAA TCTCAGAGCT GGATCAGTGC 
TCCCATAGAG TGCTAATGTA TGGCTCTGAA CTGGATGCTG ATCATCCAGG CTTTAAAGAC 
AAGGTCTATC GGCAGAGGAG AAAGTACTTT GTGGAGGTTG CAATGAACTA CAAATTTGGG 
CAGCCCATCC CACGGATTGA GTACACTGCA GAGGAAGTAA AGACATGGGG AGTCGTTTAC 
AGAGAACTTA CAAAACTCTA TCCAACTCAT GCCTGCCGCG AATACTTAAA AAACCTTCCT 
CTGCTGACAA AGCATTGTGG GTACAGAGAG GACAACATCC CACAGCTGGA GGACGTGTCT 
CTATTCTTAA GAGAGAGGTC AGGGTTTACC GTAAGGCCTG TGGCTGGATA TCTCTCACCA 
CGAGACTTCC TGGCTGGACT GGCTTATCGA GTGTTTAATT GCACTCAGTA TATTCGTCAC 
AGCACAGACC CTCTCTATAC ACCAGAGCCG GACACCTGCC ATGAACTGCT TGGTCACGTG 
CCGCTCCTGG CCGATCCCAA ATTTGCTCAG TTTTCTCAGG AGATTGGCCT TGCATCTCTA 
GGCGCTTCAG ACGAAGATGT GCAAAAGCTG GCAACTTGTT ATTTCTTCAC AATAGAGTTT 
GGGCTGTGCA AACAAGATGG TCAGTTGAGA GTCTATGGAG CAGGTCTACT GTCTTCTATT 
GGAGAGTTAA GGCATGCGCT TTCTGATAAA GCAACAGTGA AGGTGTTTGA CCCCAAAACC 
ACGTGCTACC AGGAGTGCCT CATTACCACA TTTCAAGATG TGTATTTTGT CTCTGAGAGC 
TTTGAGGAGG CAAAAGAGAA AATGAGGGAA TTTGCTAAAT CAATAAAGAG GCCTTTTTCT 
GTCTACTACA ACCCTTACAC GCAGAGCATC GACTTACTCA AGGACACCAG GAGCATTGAA 
AATGTGGTTC AAGATCTACG CAGCGACTTA ACCACCGTCT GTGACGCCCT GGGAAAAATG 
AACAAATACC TCGGTATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001296997.1
CDS	89..1729
Translation	MQGKKTSQETTATMSNELQTNPKGQQTAPKSPVGLLRKRGQEAIPKMQPAMMMFSSKYWARRGLSLDSAMYDQQHLASSMLRRTSFNRIDERPDKEEQKSTHDLGKLAVIFSLKNEVGFLVKALRLFQEKHVNLAHIESRRSKRLTNEIEIYAECNCTKKEFNELVQHLKDHVNIVSYNTPQHVWSAETDCLDCVCVLGGLPDGEGIPWFPQKISELDQCSHRVLMYGSELDADHPGFKDKVYRQRRKYFVEVAMNYKFGQPIPRIEYTAEEVKTWGVVYRELTKLYPTHACREYLKNLPLLTKHCGYREDNIPQLEDVSLFLRERSGFTVRPVAGYLSPRDFLAGLAYRVFNCTQYIRHSTDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQDGQLRVYGAGLLSSIGELRHALSDKATVKVFDPKTTCYQECLITTFQDVYFVSESFEEAKEKMREFAKSIKRPFSVYYNPYTQSIDLLKDTRSIENVVQDLRSDLTTVCDALGKMNKYLGI

Target ORF information:

RefSeq Version	NM_001310068.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) (tph2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310068.1

ORF Insert Sequence:

ATGCAAGGGA AGAAAACCTC TCAAGAGACA ACAGCAACTA TGTCTAATGA ACTGCAAACT 
AATCCAAAGG GGCAGCAAAC AGCCCCTAAA TCACCTGTTG GGCTTCTTAG GAAAAGAGGT 
CAAGAAGCAA TTCCGAAGAT GCAACCTGCC ATGATGATGT TCTCCAGCAA ATACTGGGCT 
CGGAGAGGAC TATCTTTGGA TTCAGCTATG TATGACCAAC AGCACCTTGC ATCCTCCATG 
CTCCGCAGGA CATCCTTTAA TCGGATAGAT GAAAGACCTG ACAAAGAAGA ACAGAAATCC 
ACTCATGACC TTGGGAAGCT GGCAGTGATT TTCTCTCTGA AGAATGAAGT TGGCTTTCTG 
GTGAAAGCGC TGAGGCTCTT TCAGGAGAAG CATGTGAATT TGGCGCACAT TGAGTCTCGG 
AGATCAAAGA GACTCACCAA TGAGATTGAG ATATATGCAG AATGCAACTG CACAAAGAAA 
GAGTTCAACG AACTGGTGCA ACACCTCAAA GACCACGTTA ATATTGTCTC GTATAACACA 
CCTCAACATG TGTGGTCTGC AGAGACCGAC TGTTTGGACT GTGTGTGTGT TTTGGGTGGA 
TTGCCAGATG GGGAAGGAAT ACCATGGTTT CCCCAAAAAA TCTCAGAGCT GGATCAGTGC 
TCCCATAGAG TGCTAATGTA TGGCTCTGAA CTGGATGCTG ATCATCCAGG CTTTAAAGAC 
AAGGTCTATC GGCAGAGGAG AAAGTACTTT GTGGAGGTTG CAATGAACTA CAAATTTGGG 
CAGCCCATCC CACGGATTGA GTACACTGCA GAGGAAGTAA AGACATGGGG AGTCGTTTAC 
AGAGAACTTA CAAAACTCTA TCCAACTCAT GCCTGCCGCG AATACTTAAA AAACCTTCCT 
CTGCTGACAA AGCATTGTGG GTACAGAGAG GACAACATCC CACAGCTGGA GGACGTGTCT 
CTATTCTTAA GAGAGAGGTC AGGGTTTACC GTAAGGCCTG TGGCTGGATA TCTCTCACCA 
CGAGACTTCC TGGCTGGACT GGCTTATCGA GTGTTTAATT GCACTCAGTA TATTCGTCAC 
AGCACAGACC CTCTCTATAC ACCAGAGCCG GACACCTGCC ATGAACTGCT TGGTCACGTG 
CCGCTCCTGG CCGATCCCAA ATTTGCTCAG TTTTCTCAGG AGATTGGCCT TGCATCTCTA 
GGCGCTTCAG ACGAAGATGT GCAAAAGCTG GCAACTTGTT ATTTCTTCAC AATAGAGTTT 
GGGCTGTGCA AACAAGATGG TCAGTTGAGA GTCTATGGAG CAGGTCTACT GTCTTCTATT 
GGAGAGTTAA GGCATGCGCT TTCTGATAAA GCAACAGTGA AGGTGTTTGA CCCCAAAACC 
ACGTGCTACC AGGAGTGCCT CATTACCACA TTTCAAGATG TGTATTTTGT CTCTGAGAGC 
TTTGAGGAGG CAAAAGAGAA AATGAGGGAA TTTGCTAAAT CAATAAAGAG GCCTTTTTCT 
GTCTACTACA ACCCTTACAC GCAGAGCATC GACTTACTCA AGGACACCAG GAGCATTGAA 
AATGTGGTTC AAGATCTACG CAGCGACTTA ACCACCGTCT GTGACGCCCT GGGAAAAATG 
AACAAATACC TCGGTATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tph2 ( NM_214795.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999960.2 Danio rerio tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) (tph2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa40969
Clone ID Related Accession (Same CDS sequence)	NM_214795.2
Accession Version	NM_214795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Danio rerio(zebrafish)
Product	tryptophan 5-hydroxylase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX950194.9 and EB870154.1. On Jun 25, 2015 this sequence version replaced NM_214795.1. Transcript Variant: This variant (2) differs in the 5' UTR, initiates translation at an alternate start codon, and uses an alternate splice site in the 5' coding region compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB125219.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAACA GCACCTTGCA TCCTCCATGG ACATCCTTTA ATCGGATAGA TGAAAGACCT 
GACAAAGAAG AACAGAAATC CACTCATGAC CTTGGGAAGC TGGCAGTGAT TTTCTCTCTG 
AAGAATGAAG TTGGCTTTCT GGTGAAAGCG CTGAGGCTCT TTCAGGAGAA GCATGTGAAT 
TTGGCGCACA TTGAGTCTCG GAGATCAAAG AGACTCACCA ATGAGATTGA GATATATGCA 
GAATGCAACT GCACAAAGAA AGAGTTCAAC GAACTGGTGC AACACCTCAA AGACCACGTT 
AATATTGTCT CGTATAACAC ACCTCAACAT GTGTGGTCTG CAGAGACCGA CTGTTTGGAC 
TGTGTGTGTG TTTTGGGTGG ATTGCCAGAT GGGGAAGGAA TACCATGGTT TCCCCAAAAA 
ATCTCAGAGC TGGATCAGTG CTCCCATAGA GTGCTAATGT ATGGCTCTGA ACTGGATGCT 
GATCATCCAG GCTTTAAAGA CAAGGTCTAT CGGCAGAGGA GAAAGTACTT TGTGGAGGTT 
GCAATGAACT ACAAATTTGG GCAGCCCATC CCACGGATTG AGTACACTGC AGAGGAAGTA 
AAGACATGGG GAGTCGTTTA CAGAGAACTT ACAAAACTCT ATCCAACTCA TGCCTGCCGC 
GAATACTTAA AAAACCTTCC TCTGCTGACA AAGCATTGTG GGTACAGAGA GGACAACATC 
CCACAGCTGG AGGACGTGTC TCTATTCTTA AGAGAGAGGT CAGGGTTTAC CGTAAGGCCT 
GTGGCTGGAT ATCTCTCACC ACGAGACTTC CTGGCTGGAC TGGCTTATCG AGTGTTTAAT 
TGCACTCAGT ATATTCGTCA CAGCACAGAC CCTCTCTATA CACCAGAGCC GGACACCTGC 
CATGAACTGC TTGGTCACGT GCCGCTCCTG GCCGATCCCA AATTTGCTCA GTTTTCTCAG 
GAGATTGGCC TTGCATCTCT AGGCGCTTCA GACGAAGATG TGCAAAAGCT GGCAACTTGT 
TATTTCTTCA CAATAGAGTT TGGGCTGTGC AAACAAGATG GTCAGTTGAG AGTCTATGGA 
GCAGGTCTAC TGTCTTCTAT TGGAGAGTTA AGGCATGCGC TTTCTGATAA AGCAACAGTG 
AAGGTGTTTG ACCCCAAAAC CACGTGCTAC CAGGAGTGCC TCATTACCAC ATTTCAAGAT 
GTGTATTTTG TCTCTGAGAG CTTTGAGGAG GCAAAAGAGA AAATGAGGGA ATTTGCTAAA 
TCAATAAAGA GGCCTTTTTC TGTCTACTAC AACCCTTACA CGCAGAGCAT CGACTTACTC 
AAGGACACCA GGAGCATTGA AAATGTGGTT CAAGATCTAC GCAGCGACTT AACCACCGTC 
TGTGACGCCC TGGGAAAAAT GAACAAATAC CTCGGTATCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999960.2
CDS	300..1721
Translation	MTNSTLHPPWTSFNRIDERPDKEEQKSTHDLGKLAVIFSLKNEVGFLVKALRLFQEKHVNLAHIESRRSKRLTNEIEIYAECNCTKKEFNELVQHLKDHVNIVSYNTPQHVWSAETDCLDCVCVLGGLPDGEGIPWFPQKISELDQCSHRVLMYGSELDADHPGFKDKVYRQRRKYFVEVAMNYKFGQPIPRIEYTAEEVKTWGVVYRELTKLYPTHACREYLKNLPLLTKHCGYREDNIPQLEDVSLFLRERSGFTVRPVAGYLSPRDFLAGLAYRVFNCTQYIRHSTDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFFTIEFGLCKQDGQLRVYGAGLLSSIGELRHALSDKATVKVFDPKTTCYQECLITTFQDVYFVSESFEEAKEKMREFAKSIKRPFSVYYNPYTQSIDLLKDTRSIENVVQDLRSDLTTVCDALGKMNKYLGI

Target ORF information:

RefSeq Version	NM_214795.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tryptophan hydroxylase 2 (tryptophan 5-monooxygenase) (tph2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214795.2

ORF Insert Sequence:

ATGACCAACA GCACCTTGCA TCCTCCATGG ACATCCTTTA ATCGGATAGA TGAAAGACCT 
GACAAAGAAG AACAGAAATC CACTCATGAC CTTGGGAAGC TGGCAGTGAT TTTCTCTCTG 
AAGAATGAAG TTGGCTTTCT GGTGAAAGCG CTGAGGCTCT TTCAGGAGAA GCATGTGAAT 
TTGGCGCACA TTGAGTCTCG GAGATCAAAG AGACTCACCA ATGAGATTGA GATATATGCA 
GAATGCAACT GCACAAAGAA AGAGTTCAAC GAACTGGTGC AACACCTCAA AGACCACGTT 
AATATTGTCT CGTATAACAC ACCTCAACAT GTGTGGTCTG CAGAGACCGA CTGTTTGGAC 
TGTGTGTGTG TTTTGGGTGG ATTGCCAGAT GGGGAAGGAA TACCATGGTT TCCCCAAAAA 
ATCTCAGAGC TGGATCAGTG CTCCCATAGA GTGCTAATGT ATGGCTCTGA ACTGGATGCT 
GATCATCCAG GCTTTAAAGA CAAGGTCTAT CGGCAGAGGA GAAAGTACTT TGTGGAGGTT 
GCAATGAACT ACAAATTTGG GCAGCCCATC CCACGGATTG AGTACACTGC AGAGGAAGTA 
AAGACATGGG GAGTCGTTTA CAGAGAACTT ACAAAACTCT ATCCAACTCA TGCCTGCCGC 
GAATACTTAA AAAACCTTCC TCTGCTGACA AAGCATTGTG GGTACAGAGA GGACAACATC 
CCACAGCTGG AGGACGTGTC TCTATTCTTA AGAGAGAGGT CAGGGTTTAC CGTAAGGCCT 
GTGGCTGGAT ATCTCTCACC ACGAGACTTC CTGGCTGGAC TGGCTTATCG AGTGTTTAAT 
TGCACTCAGT ATATTCGTCA CAGCACAGAC CCTCTCTATA CACCAGAGCC GGACACCTGC 
CATGAACTGC TTGGTCACGT GCCGCTCCTG GCCGATCCCA AATTTGCTCA GTTTTCTCAG 
GAGATTGGCC TTGCATCTCT AGGCGCTTCA GACGAAGATG TGCAAAAGCT GGCAACTTGT 
TATTTCTTCA CAATAGAGTT TGGGCTGTGC AAACAAGATG GTCAGTTGAG AGTCTATGGA 
GCAGGTCTAC TGTCTTCTAT TGGAGAGTTA AGGCATGCGC TTTCTGATAA AGCAACAGTG 
AAGGTGTTTG ACCCCAAAAC CACGTGCTAC CAGGAGTGCC TCATTACCAC ATTTCAAGAT 
GTGTATTTTG TCTCTGAGAG CTTTGAGGAG GCAAAAGAGA AAATGAGGGA ATTTGCTAAA 
TCAATAAAGA GGCCTTTTTC TGTCTACTAC AACCCTTACA CGCAGAGCAT CGACTTACTC 
AAGGACACCA GGAGCATTGA AAATGTGGTT CAAGATCTAC GCAGCGACTT AACCACCGTC 
TGTGACGCCC TGGGAAAAAT GAACAAATAC CTCGGTATCT AA