NMU cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	NMU
Entrez Gene ID	407657
Full Name	neuromedin U
Synonyms	si:rp71-4m17.1
General protein information	Preferred Names neuromedin U Names neuromedin-U
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305619.1	NP_001292548.1	neuromedin-U precursor
XM_021468387.1	XP_021324062.1	neuromedin-U isoform X2
XM_021468388.1	XP_021324063.1	neuromedin-U isoform X3
XM_005160513.4	XP_005160570.1	neuromedin-U isoform X1
XM_005160513.3	XP_005160570.1	neuromedin-U isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Nmu	NP_062388.1
Danio rerio (zebrafish)	nmu	XP_005160570.1
Homo sapiens (human)	NMU	NP_006672.1
Canis lupus familiaris (dog)	NMU	XP_005628261.1
Bos taurus (cattle)	NMU	NP_001178082.1
Gallus gallus (chicken)	NMU	NP_001264850.1
Xenopus tropicalis (tropical clawed frog)	nmu	XP_004911525.1
Rattus norvegicus (Norway rat)	Nmu	NP_071575.1
Pan troglodytes (chimpanzee)	NMU	XP_001142975.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes.
Kono T, Ida T, Kawahara N, Watanabe F, Biswas G, Sato T, Mori K, Miyazato M
Developmental and comparative immunology73246-256(2017 Aug)

A Zebrafish Genetic Screen Identifies Neuromedin U as a Regulator of Sleep/Wake States.
Chiu CN, Rihel J, Lee DA, Singh C, Mosser EA, Chen S, Sapin V, Pham U, Engle J, Niles BJ, Montz CJ, Chakravarthy S, Zimmerman S, Salehi-Ashtiani K, Vidal M, Schier AF, Prober DA
Neuron89(4)842-56(2016 Feb)

A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly.
Chen S, Oikonomou G, Chiu CN, Niles BJ, Liu J, Lee DA, Antoshechkin I, Prober DA
Nucleic acids research41(4)2769-78(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results reveal an unexpected functional and anatomical interface between the Nmu system and brainstem arousal systems that represents a novel wake-promoting pathway.
Title: A Zebrafish Genetic Screen Identifies Neuromedin U as a Regulator of Sleep/Wake States.

The following NMU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NMU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24796	NM_001305619.1 Latest version!	Danio rerio neuromedin U (nmu), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa24795	XM_005160513.4 Latest version!	Danio rerio neuromedin U (nmu), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa150599	XM_021468387.1 Latest version!	Danio rerio neuromedin U (nmu), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa150600	XM_021468388.1 Latest version!	Danio rerio neuromedin U (nmu), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
ODa24795	XM_005160513.3	Danio rerio neuromedin U (nmu), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NMU ( NM_001305619.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001292548.1 Danio rerio neuromedin U (nmu), mRNA.

Price & Availability↑

CloneID	ODa24796
Clone ID Related Accession (Same CDS sequence)	NM_001305619.1
Accession Version	NM_001305619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 399bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-06
Organism	Danio rerio(zebrafish)
Product	neuromedin-U precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH440630.1 and BX296520.9. On Mar 21, 2015 this sequence version replaced XM_002665712.3. ##Evidence-Data-START## Transcript exon combination :: EH440630.1, EH456573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGG AGAACCTTCA AGGTCCGGGA CGCATCCAGA GCAGAGGATA CTTCCTTTAT 
CGGCCAAGAA ATGGAAGAAG ATCTGATGAG TATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292548.1
CDS	28..426
Translation	MRNSNQCERATAHSAMSPGNTSALMLAVLLLSFIPITTSAPMLLNPSSLEHEQLLTQITDLCSFYLSADPSFRTSDVLEDLCFLMLGSLQKSKEITARETSKRENLQGPGRIQSRGYFLYRPRNGRRSDEYV

Target ORF information:

RefSeq Version	NM_001305619.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuromedin U (nmu), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305619.1

ORF Insert Sequence:

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGG AGAACCTTCA AGGTCCGGGA CGCATCCAGA GCAGAGGATA CTTCCTTTAT 
CGGCCAAGAA ATGGAAGAAG ATCTGATGAG TATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NMU ( XM_005160513.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160570.1 Danio rerio neuromedin U (nmu), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24795
Clone ID Related Accession (Same CDS sequence)	XM_005160513.4 , XM_005160513.3
Accession Version	XM_005160513.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuromedin-U isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160513.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGT TTTTATTTCA TTACACTAAA CCAAACGGGG CAGGATTGTC TGATGGGACG 
TCTACTGTGT TGCACCCTCT TCTGGAGCTC ATACCCCAGC TTGCCAGAAG AAGAAGCAGG 
AGAATGAAAT TAAATGAGAA CCTTCAAGGT CCGGGACGCA TCCAGAGCAG AGGATACTTC 
CTTTATCGGC CAAGAAATGG AAGAAGATCT GATGAGTATG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160570.1
CDS	53..577
Translation	MRNSNQCERATAHSAMSPGNTSALMLAVLLLSFIPITTSAPMLLNPSSLEHEQLLTQITDLCSFYLSADPSFRTSDVLEDLCFLMLGSLQKSKEITARETSKRFLFHYTKPNGAGLSDGTSTVLHPLLELIPQLARRRSRRMKLNENLQGPGRIQSRGYFLYRPRNGRRSDEYV

Target ORF information:

RefSeq Version	XM_005160513.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuromedin U (nmu), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160513.4

ORF Insert Sequence:

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGT TTTTATTTCA TTACACTAAA CCAAACGGGG CAGGATTGTC TGATGGGACG 
TCTACTGTGT TGCACCCTCT TCTGGAGCTC ATACCCCAGC TTGCCAGAAG AAGAAGCAGG 
AGAATGAAAT TAAATGAGAA CCTTCAAGGT CCGGGACGCA TCCAGAGCAG AGGATACTTC 
CTTTATCGGC CAAGAAATGG AAGAAGATCT GATGAGTATG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NMU ( XM_005160513.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160570.1 Danio rerio neuromedin U (nmu), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24795
Clone ID Related Accession (Same CDS sequence)	XM_005160513.4 , XM_005160513.3
Accession Version	XM_005160513.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neuromedin-U isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160513.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGT TTTTATTTCA TTACACTAAA CCAAACGGGG CAGGATTGTC TGATGGGACG 
TCTACTGTGT TGCACCCTCT TCTGGAGCTC ATACCCCAGC TTGCCAGAAG AAGAAGCAGG 
AGAATGAAAT TAAATGAGAA CCTTCAAGGT CCGGGACGCA TCCAGAGCAG AGGATACTTC 
CTTTATCGGC CAAGAAATGG AAGAAGATCT GATGAGTATG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160570.1
CDS	15..539
Translation	MRNSNQCERATAHSAMSPGNTSALMLAVLLLSFIPITTSAPMLLNPSSLEHEQLLTQITDLCSFYLSADPSFRTSDVLEDLCFLMLGSLQKSKEITARETSKRFLFHYTKPNGAGLSDGTSTVLHPLLELIPQLARRRSRRMKLNENLQGPGRIQSRGYFLYRPRNGRRSDEYV

Target ORF information:

RefSeq Version	XM_005160513.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuromedin U (nmu), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160513.3

ORF Insert Sequence:

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGT TTTTATTTCA TTACACTAAA CCAAACGGGG CAGGATTGTC TGATGGGACG 
TCTACTGTGT TGCACCCTCT TCTGGAGCTC ATACCCCAGC TTGCCAGAAG AAGAAGCAGG 
AGAATGAAAT TAAATGAGAA CCTTCAAGGT CCGGGACGCA TCCAGAGCAG AGGATACTTC 
CTTTATCGGC CAAGAAATGG AAGAAGATCT GATGAGTATG TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NMU ( XM_021468387.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021324062.1 Danio rerio neuromedin U (nmu), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150599
Clone ID Related Accession (Same CDS sequence)	XM_021468387.1
Accession Version	XM_021468387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuromedin-U isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GACATCTGAC 
GTCCTGGAGG ACCTGTGTTT CCTAATGCTG GGATCACTGC AAAAATCGAA GGAGATCACA 
GCTCGAGAGA CTAGCAAAAG GTTTTTATTT CATTACACTA AACCAAACGG GGCAGGATTG 
TCTGATGGGA CGTCTACTGT GTTGCACCCT CTTCTGGAGC TCATACCCCA GCTTGCCAGA 
AGAAGAAGCA GGAGAATGAA ATTAAATGAG AACCTTCAAG GTCCGGGACG CATCCAGAGC 
AGAGGATACT TCCTTTATCG GCCAAGAAAT GGAAGAAGAT CTGATGAGTA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324062.1
CDS	55..531
Translation	MRNSNQCERATAHSAMSPGNTSALMLAVLLLSFIPITTSAPMLLNPSSLEHEQLLTQTSDVLEDLCFLMLGSLQKSKEITARETSKRFLFHYTKPNGAGLSDGTSTVLHPLLELIPQLARRRSRRMKLNENLQGPGRIQSRGYFLYRPRNGRRSDEYV

Target ORF information:

RefSeq Version	XM_021468387.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuromedin U (nmu), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468387.1

ORF Insert Sequence:

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GACATCTGAC 
GTCCTGGAGG ACCTGTGTTT CCTAATGCTG GGATCACTGC AAAAATCGAA GGAGATCACA 
GCTCGAGAGA CTAGCAAAAG GTTTTTATTT CATTACACTA AACCAAACGG GGCAGGATTG 
TCTGATGGGA CGTCTACTGT GTTGCACCCT CTTCTGGAGC TCATACCCCA GCTTGCCAGA 
AGAAGAAGCA GGAGAATGAA ATTAAATGAG AACCTTCAAG GTCCGGGACG CATCCAGAGC 
AGAGGATACT TCCTTTATCG GCCAAGAAAT GGAAGAAGAT CTGATGAGTA TGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NMU ( XM_021468388.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021324063.1 Danio rerio neuromedin U (nmu), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa150600
Clone ID Related Accession (Same CDS sequence)	XM_021468388.1
Accession Version	XM_021468388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuromedin-U isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGT TTTTATTTCA TTACACTAAA CCAAACGGGG CAGGATTGTC TGATGGGACG 
GAGAACCTTC AAGGTCCGGG ACGCATCCAG AGCAGAGGAT ACTTCCTTTA TCGGCCAAGA 
AATGGAAGAA GATCTGATGA GTATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324063.1
CDS	52..501
Translation	MRNSNQCERATAHSAMSPGNTSALMLAVLLLSFIPITTSAPMLLNPSSLEHEQLLTQITDLCSFYLSADPSFRTSDVLEDLCFLMLGSLQKSKEITARETSKRFLFHYTKPNGAGLSDGTENLQGPGRIQSRGYFLYRPRNGRRSDEYV

Target ORF information:

RefSeq Version	XM_021468388.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuromedin U (nmu), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468388.1

ORF Insert Sequence:

ATGAGGAACA GCAATCAATG TGAACGCGCA ACCGCTCACA GCGCGATGAG CCCGGGAAAC 
ACCTCCGCCC TGATGCTCGC GGTACTACTC CTCTCCTTCA TACCCATCAC CACAAGTGCT 
CCGATGCTCT TGAATCCATC TTCACTAGAG CATGAGCAGC TACTAACCCA GATAACTGAT 
TTGTGTTCAT TCTACCTCTC CGCAGACCCG TCCTTTAGAA CATCTGACGT CCTGGAGGAC 
CTGTGTTTCC TAATGCTGGG ATCACTGCAA AAATCGAAGG AGATCACAGC TCGAGAGACT 
AGCAAAAGGT TTTTATTTCA TTACACTAAA CCAAACGGGG CAGGATTGTC TGATGGGACG 
GAGAACCTTC AAGGTCCGGG ACGCATCCAG AGCAGAGGAT ACTTCCTTTA TCGGCCAAGA 
AATGGAAGAA GATCTGATGA GTATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and immunoregulatory function of neuromedin U (Nmu) in the Japanese pufferfish Takifugu rubripes.
Developmental and comparative immunology73246-256(2017 Aug)
Kono T,Ida T,Kawahara N,Watanabe F,Biswas G,Sato T,Mori K,Miyazato M

A Zebrafish Genetic Screen Identifies Neuromedin U as a Regulator of Sleep/Wake States.
Neuron89(4)842-56(2016 Feb)
Chiu CN,Rihel J,Lee DA,Singh C,Mosser EA,Chen S,Sapin V,Pham U,Engle J,Niles BJ,Montz CJ,Chakravarthy S,Zimmerman S,Salehi-Ashtiani K,Vidal M,Schier AF,Prober DA

A large-scale in vivo analysis reveals that TALENs are significantly more mutagenic than ZFNs generated using context-dependent assembly.
Nucleic acids research41(4)2769-78(2013 Feb)
Chen S,Oikonomou G,Chiu CN,Niles BJ,Liu J,Lee DA,Antoshechkin I,Prober DA

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NMU cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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