tcn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tcn2
Entrez Gene ID	407646
Full Name	transcobalamin II
Synonyms	si:ch211-102c2.6,tcn2l,wu:fd46e10
General protein information	Preferred Names transcobalamin II Names transcobalamin-2 transcobalamin II, like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001123231.2	NP_001116703.1	transcobalamin-2 precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TCN2	NP_776620.2
Pan troglodytes (chimpanzee)	TCN2	XP_525562.1
	TCN2	XP_001110286.1
Mus musculus (house mouse)	Tcn2	NP_001123930.1
Xenopus tropicalis (tropical clawed frog)	tcn2	NP_001184035.1
Homo sapiens (human)	TCN2	NP_000346.2
Canis lupus familiaris (dog)	TCN2	XP_543481.3
Rattus norvegicus (Norway rat)	Tcn2	NP_071979.1
Gallus gallus (chicken)	TCN2	XP_427292.3
Danio rerio (zebrafish)	tcn2l	NP_001116703.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The cobalamin-binding protein in zebrafish is an intermediate between the three cobalamin-binding proteins in human.
Greibe E, Fedosov S, Nexo E
PloS one7(4)e35660(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34223	NM_001123231.2 Latest version!	Danio rerio transcobalamin II (tcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tcn2 ( NM_001123231.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001116703.1 Danio rerio transcobalamin II (tcn2), mRNA.

Price & Availability↑

CloneID	ODa34223
Clone ID Related Accession (Same CDS sequence)	NM_001123231.2
Accession Version	NM_001123231.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-30
Organism	Danio rerio(zebrafish)
Product	transcobalamin-2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU041374.12, EH509290.1 and EH535398.1. On Aug 12, 2010 this sequence version replaced NM_001123231.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01003526.1, GDQQ01000683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGATTACTC TCCTCATTGT GAGTGCGCTG CTGGCACTGG CTGCTGGAAA ACCCTGTGCA 
TCAGATCACG AAACTCTTCT CCAGTCGCTC AATAAGCAGC TTCTGCGCTC TGTGGACACA 
CAAGACAACC TGCCCAATCC CAGTGTGCAC ATCGCTCTGC GGCTCTCCAC ACAACATAAC 
CTGGACAAAG AAAACCAATA TCTCAACCGC CTCAAGAAAG AGTTTCATGA GGATATTGAG 
AAATCTCTGA GAAATGGTGA GTTGGTGGTT GGCCGTCTCG CCCTGTACAT CTTGGCCTTG 
AGGTCCTCCT GTCACGATCT GAGTTTACAC CTCAATCACA ATGAGAAGAA TGAGTTCCTC 
CTAACTCACC TTAAGAAAGA AATGGAGGAA GAGAAACAAA ACATTGCATT CAGTCATCGT 
CCGAAGACCA ACTATTACCA GTATTCTCTG GGTATTCTGG CTTTGTGTGT GAGCGGAGTT 
AGAGTTAGCA CACACGTCAG CCACAAGCTC ATCCATGCTG TGGAACATGG ACAAATAAAA 
CACGGAGAGT CCTTATGCAT TGACTCACAT GCGATGGCAG GCATGGCCCT GCAGTGTCTA 
AAAAATGAGG GAATCTCGGT TAAAGATGAA GCTGAGCTGG ACAAAGCTTT GGCTACCATT 
AAGCAGAAGC TTGTAGACTC TAAACGAGCT GATGGTCACA TGGGCAATGA ATTCAGCACG 
GGCTTGGCAG TGCAGGCCTT GTTGGCTATG GGAGTTGAGA TGGAGGAATG CGGCACTGCT 
ATCGAGGCTT TAAGGGGAGA TATCAGGAAG GGAACATACC ATAATCCAAT GGCAGCATCC 
CAAGTTCTGC CTGCCCTATA CCAGCAGTCC TATCTTCATC TGAAGAGCAA AGAATGCCGC 
AGTGAGGACG ACACTCTTAC TGCTGATGTT GAATCGGCTT CAGAGGTTCT CCCAAGTCTC 
GGACAAGTGG CTGTACAAGT GGAGGTTATA AAATCCAATG GGGAAGCGTC TGTGTTTCCC 
ATTAATGTCC CCAAAGGCTC CTCTCTGTTT GAAGCCCTGA ACCTTCTTCA AGACAAACAA 
ACTGGTTTCA CCTTTAAGAC TGAAGACAGC TTGTGGGGAG CTTTTCTCAG TGTGCTCAAT 
GATGAGCAAG CCCGTCAGAC TGATCGCAGA TACTGGCATG TTTCCTCAGA TGGCACCTCT 
CTGACACAGG GTATTAAAGA CTATAAGATT GACTCAGCTC AGCGCATCAC CATTAAAAAC 
ACTGGCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116703.1
CDS	262..1533
Translation	MITLLIVSALLALAAGKPCASDHETLLQSLNKQLLRSVDTQDNLPNPSVHIALRLSTQHNLDKENQYLNRLKKEFHEDIEKSLRNGELVVGRLALYILALRSSCHDLSLHLNHNEKNEFLLTHLKKEMEEEKQNIAFSHRPKTNYYQYSLGILALCVSGVRVSTHVSHKLIHAVEHGQIKHGESLCIDSHAMAGMALQCLKNEGISVKDEAELDKALATIKQKLVDSKRADGHMGNEFSTGLAVQALLAMGVEMEECGTAIEALRGDIRKGTYHNPMAASQVLPALYQQSYLHLKSKECRSEDDTLTADVESASEVLPSLGQVAVQVEVIKSNGEASVFPINVPKGSSLFEALNLLQDKQTGFTFKTEDSLWGAFLSVLNDEQARQTDRRYWHVSSDGTSLTQGIKDYKIDSAQRITIKNTGY

Target ORF information:

RefSeq Version	NM_001123231.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transcobalamin II (tcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123231.2

ORF Insert Sequence:

ATGATTACTC TCCTCATTGT GAGTGCGCTG CTGGCACTGG CTGCTGGAAA ACCCTGTGCA 
TCAGATCACG AAACTCTTCT CCAGTCGCTC AATAAGCAGC TTCTGCGCTC TGTGGACACA 
CAAGACAACC TGCCCAATCC CAGTGTGCAC ATCGCTCTGC GGCTCTCCAC ACAACATAAC 
CTGGACAAAG AAAACCAATA TCTCAACCGC CTCAAGAAAG AGTTTCATGA GGATATTGAG 
AAATCTCTGA GAAATGGTGA GTTGGTGGTT GGCCGTCTCG CCCTGTACAT CTTGGCCTTG 
AGGTCCTCCT GTCACGATCT GAGTTTACAC CTCAATCACA ATGAGAAGAA TGAGTTCCTC 
CTAACTCACC TTAAGAAAGA AATGGAGGAA GAGAAACAAA ACATTGCATT CAGTCATCGT 
CCGAAGACCA ACTATTACCA GTATTCTCTG GGTATTCTGG CTTTGTGTGT GAGCGGAGTT 
AGAGTTAGCA CACACGTCAG CCACAAGCTC ATCCATGCTG TGGAACATGG ACAAATAAAA 
CACGGAGAGT CCTTATGCAT TGACTCACAT GCGATGGCAG GCATGGCCCT GCAGTGTCTA 
AAAAATGAGG GAATCTCGGT TAAAGATGAA GCTGAGCTGG ACAAAGCTTT GGCTACCATT 
AAGCAGAAGC TTGTAGACTC TAAACGAGCT GATGGTCACA TGGGCAATGA ATTCAGCACG 
GGCTTGGCAG TGCAGGCCTT GTTGGCTATG GGAGTTGAGA TGGAGGAATG CGGCACTGCT 
ATCGAGGCTT TAAGGGGAGA TATCAGGAAG GGAACATACC ATAATCCAAT GGCAGCATCC 
CAAGTTCTGC CTGCCCTATA CCAGCAGTCC TATCTTCATC TGAAGAGCAA AGAATGCCGC 
AGTGAGGACG ACACTCTTAC TGCTGATGTT GAATCGGCTT CAGAGGTTCT CCCAAGTCTC 
GGACAAGTGG CTGTACAAGT GGAGGTTATA AAATCCAATG GGGAAGCGTC TGTGTTTCCC 
ATTAATGTCC CCAAAGGCTC CTCTCTGTTT GAAGCCCTGA ACCTTCTTCA AGACAAACAA 
ACTGGTTTCA CCTTTAAGAC TGAAGACAGC TTGTGGGGAG CTTTTCTCAG TGTGCTCAAT 
GATGAGCAAG CCCGTCAGAC TGATCGCAGA TACTGGCATG TTTCCTCAGA TGGCACCTCT 
CTGACACAGG GTATTAAAGA CTATAAGATT GACTCAGCTC AGCGCATCAC CATTAAAAAC 
ACTGGCTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The cobalamin-binding protein in zebrafish is an intermediate between the three cobalamin-binding proteins in human.
PloS one7(4)e35660(2012)
Greibe E,Fedosov S,Nexo E