sass6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sass6
Entrez Gene ID	406747
Full Name	SAS-6 centriolar assembly protein
Synonyms	wu:fb98c12,zgc:55668
General protein information	Preferred Names SAS-6 centriolar assembly protein Names spindle assembly abnormal protein 6 homolog cea cellular atoll spindle assembly 6 homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005173944.3	XP_005174001.1	spindle assembly abnormal protein 6 homolog isoform X1
XM_009296095.2	XP_009294370.1	spindle assembly abnormal protein 6 homolog isoform X2
XM_009296095.3	XP_009294370.1	spindle assembly abnormal protein 6 homolog isoform X2
NM_213438.1	NP_998603.1	spindle assembly abnormal protein 6 homolog
XM_005173944.4	XP_005174001.1	spindle assembly abnormal protein 6 homolog isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SASS6	NP_001178955.1
Xenopus tropicalis (tropical clawed frog)	sass6	NP_001039141.1
Homo sapiens (human)	SASS6	NP_919268.1
Pan troglodytes (chimpanzee)	SASS6	XP_513588.2
Mus musculus (house mouse)	Sass6	NP_082625.2
Danio rerio (zebrafish)	sass6	NP_998603.1
	SASS6	XP_001106791.2
Canis lupus familiaris (dog)	SASS6	XP_547263.4
Rattus norvegicus (Norway rat)	Sass6	NP_001101189.1
Gallus gallus (chicken)	SASS6	NP_001026444.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

In vitro oocyte culture-based manipulation of zebrafish maternal genes.
Nair S, Lindeman RE, Pelegri F
Developmental dynamics : an official publication of the American Association of Anatomists242(1)44-52(2013 Jan)

Insm1a-mediated gene repression is essential for the formation and differentiation of M?ller glia-derived progenitors in the injured retina.
Ramachandran R, Zhao XF, Goldman D
Nature cell biology14(10)1013-23(2012 Oct)

Structures of SAS-6 suggest its organization in centrioles.
van Breugel M, Hirono M, Andreeva A, Yanagisawa HA, Yamaguchi S, Nakazawa Y, Morgner N, Petrovich M, Ebong IO, Robinson CV, Johnson CM, Veprintsev D, Zuber B
Science (New York, N.Y.)331(6021)1196-9(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study determined the x-ray structure of the amino-terminal domain of SAS-6 and showed that recombinant SAS-6 self-associates in vitro into assemblies that resemble cartwheel centers
Title: Structures of SAS-6 suggest its organization in centrioles.

cea encodes the centriolar coiled-coil protein Sas-6, and that zebrafish Cea/Sas-6 protein localizes to centrosomes.[Cea]
Title: The zebrafish maternal-effect gene cellular atoll encodes the centriolar component sas-6 and defects in its paternal function promote whole genome duplication.

The following sass6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sass6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa37718	NM_213438.1 Latest version!	Danio rerio SAS-6 centriolar assembly protein (sass6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26670	XM_005173944.4 Latest version!	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa40915	XM_009296095.3 Latest version!	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa40915	XM_009296095.2	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa26670	XM_005173944.3	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sass6 ( NM_213438.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998603.1 Danio rerio SAS-6 centriolar assembly protein (sass6), mRNA.

Price & Availability↑

CloneID	ODa37718
Clone ID Related Accession (Same CDS sequence)	NM_213438.1
Accession Version	NM_213438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	spindle assembly abnormal protein 6 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045420.1. ##Evidence-Data-START## Transcript exon combination :: BC045420.1, BC165167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAGCCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTC CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGG CTTTCCCCAT CACCTCTACC 
ATAAACTCCA AATATCCTCT TGCCCTGTCA TGTGTGAGCT CAGGATCTCG ATCTGTCCTG 
ACCAGCAGCA ATGGACCCAA GGTCCAGTTT AACCCGATGA GTGTCAAACC TTCAGCAGCA 
GAGGTGAGTC CAGCTGCTTT TTCCCAGCCG GCCAATAAAG AGAACAGTGA ACCTGTAGGA 
TTGGACTCAA AGTACTTTGA GAGACGAGAT GACAGCATTC CTCTTCGTGG CCTTCTACCC 
AGCATGCATC TAAATAGAGA AGTTCCAAAG CCACTGAACA CAGCTGCAGC GAAAGCAACA 
CCATCAGCGT TTTTTCCAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998603.1
CDS	90..1973
Translation	MTELLFNKRLQVLVKSKDTDERRSVIRVSIELQLPSSPVHRKDLVVRLTDDTDLYFLYNLIISEEDFQSLKVQQGLLIDFTSFPQKFIDLLEQCICEQDKESPRFLLQLSSSSSAFDHSPSNLNIVETNAFKHLTHLSLKLLPGSDTDIKKYLASCLSSVKEEKQQLQQKLRKTEEDLTRQLNYAQQTLSEKSRELDKLRSEWTSQTTSLSSRHMQDLTAEREKALETQSRLQQQNEQLRQELESSHHRSTQQLQTKVSELETANRELIDKKYKSDSTIRDLKAKLTSLEEECQRSKQQVLSLRRENSALDSECHEKERLLNQLQTRVAVLEQEIKDKDQLVLRTKEVLEATQQQKNSVEGNAESKQLQISKLESTVKSLSEELIKANGIIKKLQADLKALLGKIKVKNSVTVPQEKILQETSDKLQRQQRELQDTQQRLSLKEEEAAKLKEQLEATVQKLDESREVLKTNENVITWLNKQLNENQLSRKQETVAMFETPAAALRSAAVPHNMAFPITSTINSKYPLALSCVSSGSRSVLTSSNGPKVQFNPMSVKPSAAEVSPAAFSQPANKENSEPVGLDSKYFERRDDSIPLRGLLPSMHLNREVPKPLNTAAAKATPSAFFPG

Target ORF information:

RefSeq Version	NM_213438.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAS-6 centriolar assembly protein (sass6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213438.1

ORF Insert Sequence:

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAGCCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTC CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGG CTTTCCCCAT CACCTCTACC 
ATAAACTCCA AATATCCTCT TGCCCTGTCA TGTGTGAGCT CAGGATCTCG ATCTGTCCTG 
ACCAGCAGCA ATGGACCCAA GGTCCAGTTT AACCCGATGA GTGTCAAACC TTCAGCAGCA 
GAGGTGAGTC CAGCTGCTTT TTCCCAGCCG GCCAATAAAG AGAACAGTGA ACCTGTAGGA 
TTGGACTCAA AGTACTTTGA GAGACGAGAT GACAGCATTC CTCTTCGTGG CCTTCTACCC 
AGCATGCATC TAAATAGAGA AGTTCCAAAG CCACTGAACA CAGCTGCAGC GAAAGCAACA 
CCATCAGCGT TTTTTCCAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sass6 ( XM_005173944.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174001.1 Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26670
Clone ID Related Accession (Same CDS sequence)	XM_005173944.3 , XM_005173944.4
Accession Version	XM_005173944.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	spindle assembly abnormal protein 6 homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGC TTGATAGTAA AGGTTTGTTG 
TCGTCTTTGG GACAGGCTTT CCCCATCACC TCTACCATAA ACTCCAAATA TCCTCTTGCC 
CTGTCATGTG TGAGCTCAGG ATCTCGATCT GTCCTGACCA GCAGCAATGG ACCCAAGGTC 
CAGTTTAACC CGATGAGTGT CAAACCTTCA GCAGCAGAGG TGAGTCCAGC TGCTTTTTCC 
CAGCCGGCCA ATAAAGAGAA CAGTGAACCT GTAGGATTGG ACTCAAAGTA CTTTGAGAGA 
CGAGATGACA GCATTCCTCT TCGTGGCCTT CTACCCAGCA TGCATCTAAA TAGAGAAGTT 
CCAAAGCCAC TGAACACAGC TGCAGCGAAA GCAACACCAT CAGCGTTTTT TCCAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174001.1
CDS	84..2003
Translation	MTELLFNKRLQVLVKSKDTDERRSVIRVSIELQLPSSPVHRKDLVVRLTDDTDLYFLYNLIISEEDFQSLKVQQGLLIDFTSFPQKFIDLLEQCICEQDKENPRFLLQLSSSSSAFDHSPSNLNIVETNAFKHLTHLSLKLLPGSDTDIKKYLASCLSSVKEEKQQLQQKLRKTEEDLTRQLNYAQQTLSEKSRELDKLRSEWTSQTTSLSSRHMQDLTAEREKALETQSRLQQQNEQLRQELESSHHRSTQQLQTKVSELETANRELIDKKYKSDSTIRDLKAKLTSLEEECQRSKQQVLSLRRENSALDSECHEKERLLNQLQTRVAVLEQEIKDKDQLVLRTKEVLEATQQQKNSVEGNAESKQLQISKLESTVKSLSEELIKANGIIKKLQADLKALLGKIKVKNSVTVSQEKILQETSDKLQRQQRELQDTQQRLSLKEEEAAKLKEQLEATVQKLDESREVLKTNENVITWLNKQLNENQLSRKQETVAMFETPAAALRSAAVPHNMLDSKGLLSSLGQAFPITSTINSKYPLALSCVSSGSRSVLTSSNGPKVQFNPMSVKPSAAEVSPAAFSQPANKENSEPVGLDSKYFERRDDSIPLRGLLPSMHLNREVPKPLNTAAAKATPSAFFPG

Target ORF information:

RefSeq Version	XM_005173944.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173944.3

ORF Insert Sequence:

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGC TTGATAGTAA AGGTTTGTTG 
TCGTCTTTGG GACAGGCTTT CCCCATCACC TCTACCATAA ACTCCAAATA TCCTCTTGCC 
CTGTCATGTG TGAGCTCAGG ATCTCGATCT GTCCTGACCA GCAGCAATGG ACCCAAGGTC 
CAGTTTAACC CGATGAGTGT CAAACCTTCA GCAGCAGAGG TGAGTCCAGC TGCTTTTTCC 
CAGCCGGCCA ATAAAGAGAA CAGTGAACCT GTAGGATTGG ACTCAAAGTA CTTTGAGAGA 
CGAGATGACA GCATTCCTCT TCGTGGCCTT CTACCCAGCA TGCATCTAAA TAGAGAAGTT 
CCAAAGCCAC TGAACACAGC TGCAGCGAAA GCAACACCAT CAGCGTTTTT TCCAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sass6 ( XM_005173944.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174001.1 Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26670
Clone ID Related Accession (Same CDS sequence)	XM_005173944.3 , XM_005173944.4
Accession Version	XM_005173944.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	spindle assembly abnormal protein 6 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173944.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGC TTGATAGTAA AGGTTTGTTG 
TCGTCTTTGG GACAGGCTTT CCCCATCACC TCTACCATAA ACTCCAAATA TCCTCTTGCC 
CTGTCATGTG TGAGCTCAGG ATCTCGATCT GTCCTGACCA GCAGCAATGG ACCCAAGGTC 
CAGTTTAACC CGATGAGTGT CAAACCTTCA GCAGCAGAGG TGAGTCCAGC TGCTTTTTCC 
CAGCCGGCCA ATAAAGAGAA CAGTGAACCT GTAGGATTGG ACTCAAAGTA CTTTGAGAGA 
CGAGATGACA GCATTCCTCT TCGTGGCCTT CTACCCAGCA TGCATCTAAA TAGAGAAGTT 
CCAAAGCCAC TGAACACAGC TGCAGCGAAA GCAACACCAT CAGCGTTTTT TCCAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174001.1
CDS	89..2008
Translation	MTELLFNKRLQVLVKSKDTDERRSVIRVSIELQLPSSPVHRKDLVVRLTDDTDLYFLYNLIISEEDFQSLKVQQGLLIDFTSFPQKFIDLLEQCICEQDKENPRFLLQLSSSSSAFDHSPSNLNIVETNAFKHLTHLSLKLLPGSDTDIKKYLASCLSSVKEEKQQLQQKLRKTEEDLTRQLNYAQQTLSEKSRELDKLRSEWTSQTTSLSSRHMQDLTAEREKALETQSRLQQQNEQLRQELESSHHRSTQQLQTKVSELETANRELIDKKYKSDSTIRDLKAKLTSLEEECQRSKQQVLSLRRENSALDSECHEKERLLNQLQTRVAVLEQEIKDKDQLVLRTKEVLEATQQQKNSVEGNAESKQLQISKLESTVKSLSEELIKANGIIKKLQADLKALLGKIKVKNSVTVSQEKILQETSDKLQRQQRELQDTQQRLSLKEEEAAKLKEQLEATVQKLDESREVLKTNENVITWLNKQLNENQLSRKQETVAMFETPAAALRSAAVPHNMLDSKGLLSSLGQAFPITSTINSKYPLALSCVSSGSRSVLTSSNGPKVQFNPMSVKPSAAEVSPAAFSQPANKENSEPVGLDSKYFERRDDSIPLRGLLPSMHLNREVPKPLNTAAAKATPSAFFPG

Target ORF information:

RefSeq Version	XM_005173944.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173944.4

ORF Insert Sequence:

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGC TTGATAGTAA AGGTTTGTTG 
TCGTCTTTGG GACAGGCTTT CCCCATCACC TCTACCATAA ACTCCAAATA TCCTCTTGCC 
CTGTCATGTG TGAGCTCAGG ATCTCGATCT GTCCTGACCA GCAGCAATGG ACCCAAGGTC 
CAGTTTAACC CGATGAGTGT CAAACCTTCA GCAGCAGAGG TGAGTCCAGC TGCTTTTTCC 
CAGCCGGCCA ATAAAGAGAA CAGTGAACCT GTAGGATTGG ACTCAAAGTA CTTTGAGAGA 
CGAGATGACA GCATTCCTCT TCGTGGCCTT CTACCCAGCA TGCATCTAAA TAGAGAAGTT 
CCAAAGCCAC TGAACACAGC TGCAGCGAAA GCAACACCAT CAGCGTTTTT TCCAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sass6 ( XM_009296095.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294370.1 Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40915
Clone ID Related Accession (Same CDS sequence)	XM_009296095.2 , XM_009296095.3
Accession Version	XM_009296095.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	spindle assembly abnormal protein 6 homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296095.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGG TCCAGTTTAA CCCGATGAGT 
GTCAAACCTT CAGCAGCAGA GGTGAGTCCA GCTGCTTTTT CCCAGCCGGC CAATAAAGAG 
AACAGTGAAC CTGTAGGATT GGACTCAAAG TACTTTGAGA GACGAGATGA CAGCATTCCT 
CTTCGTGGCC TTCTACCCAG CATGCATCTA AATAGAGAAG TTCCAAAGCC ACTGAACACA 
GCTGCAGCGA AAGCAACACC ATCAGCGTTT TTTCCAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294370.1
CDS	84..1865
Translation	MTELLFNKRLQVLVKSKDTDERRSVIRVSIELQLPSSPVHRKDLVVRLTDDTDLYFLYNLIISEEDFQSLKVQQGLLIDFTSFPQKFIDLLEQCICEQDKENPRFLLQLSSSSSAFDHSPSNLNIVETNAFKHLTHLSLKLLPGSDTDIKKYLASCLSSVKEEKQQLQQKLRKTEEDLTRQLNYAQQTLSEKSRELDKLRSEWTSQTTSLSSRHMQDLTAEREKALETQSRLQQQNEQLRQELESSHHRSTQQLQTKVSELETANRELIDKKYKSDSTIRDLKAKLTSLEEECQRSKQQVLSLRRENSALDSECHEKERLLNQLQTRVAVLEQEIKDKDQLVLRTKEVLEATQQQKNSVEGNAESKQLQISKLESTVKSLSEELIKANGIIKKLQADLKALLGKIKVKNSVTVSQEKILQETSDKLQRQQRELQDTQQRLSLKEEEAAKLKEQLEATVQKLDESREVLKTNENVITWLNKQLNENQLSRKQETVAMFETPAAALRSAAVPHNMVQFNPMSVKPSAAEVSPAAFSQPANKENSEPVGLDSKYFERRDDSIPLRGLLPSMHLNREVPKPLNTAAAKATPSAFFPG

Target ORF information:

RefSeq Version	XM_009296095.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296095.2

ORF Insert Sequence:

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGG TCCAGTTTAA CCCGATGAGT 
GTCAAACCTT CAGCAGCAGA GGTGAGTCCA GCTGCTTTTT CCCAGCCGGC CAATAAAGAG 
AACAGTGAAC CTGTAGGATT GGACTCAAAG TACTTTGAGA GACGAGATGA CAGCATTCCT 
CTTCGTGGCC TTCTACCCAG CATGCATCTA AATAGAGAAG TTCCAAAGCC ACTGAACACA 
GCTGCAGCGA AAGCAACACC ATCAGCGTTT TTTCCAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sass6 ( XM_009296095.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294370.1 Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40915
Clone ID Related Accession (Same CDS sequence)	XM_009296095.2 , XM_009296095.3
Accession Version	XM_009296095.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	spindle assembly abnormal protein 6 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGG TCCAGTTTAA CCCGATGAGT 
GTCAAACCTT CAGCAGCAGA GGTGAGTCCA GCTGCTTTTT CCCAGCCGGC CAATAAAGAG 
AACAGTGAAC CTGTAGGATT GGACTCAAAG TACTTTGAGA GACGAGATGA CAGCATTCCT 
CTTCGTGGCC TTCTACCCAG CATGCATCTA AATAGAGAAG TTCCAAAGCC ACTGAACACA 
GCTGCAGCGA AAGCAACACC ATCAGCGTTT TTTCCAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294370.1
CDS	89..1870
Translation	MTELLFNKRLQVLVKSKDTDERRSVIRVSIELQLPSSPVHRKDLVVRLTDDTDLYFLYNLIISEEDFQSLKVQQGLLIDFTSFPQKFIDLLEQCICEQDKENPRFLLQLSSSSSAFDHSPSNLNIVETNAFKHLTHLSLKLLPGSDTDIKKYLASCLSSVKEEKQQLQQKLRKTEEDLTRQLNYAQQTLSEKSRELDKLRSEWTSQTTSLSSRHMQDLTAEREKALETQSRLQQQNEQLRQELESSHHRSTQQLQTKVSELETANRELIDKKYKSDSTIRDLKAKLTSLEEECQRSKQQVLSLRRENSALDSECHEKERLLNQLQTRVAVLEQEIKDKDQLVLRTKEVLEATQQQKNSVEGNAESKQLQISKLESTVKSLSEELIKANGIIKKLQADLKALLGKIKVKNSVTVSQEKILQETSDKLQRQQRELQDTQQRLSLKEEEAAKLKEQLEATVQKLDESREVLKTNENVITWLNKQLNENQLSRKQETVAMFETPAAALRSAAVPHNMVQFNPMSVKPSAAEVSPAAFSQPANKENSEPVGLDSKYFERRDDSIPLRGLLPSMHLNREVPKPLNTAAAKATPSAFFPG

Target ORF information:

RefSeq Version	XM_009296095.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SAS-6 centriolar assembly protein (sass6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296095.3

ORF Insert Sequence:

ATGACGGAAT TACTTTTTAA CAAGCGGTTG CAGGTGCTGG TGAAGAGTAA AGATACCGAT 
GAGAGGCGTT CGGTTATTCG CGTTAGCATT GAACTGCAGT TACCATCCAG CCCTGTTCAC 
AGAAAGGATT TAGTTGTACG GCTGACTGAT GATACTGATC TTTACTTTTT ATACAACCTT 
ATCATATCAG AGGAGGATTT TCAAAGCTTA AAAGTACAAC AAGGGCTTTT AATAGACTTC 
ACATCATTCC CACAAAAATT TATAGACTTA CTTGAACAGT GCATTTGCGA ACAGGACAAA 
GAAAACCCAC GGTTTCTTTT ACAGCTCTCG TCATCTTCAT CAGCGTTCGA TCACAGTCCT 
TCAAACCTCA ATATTGTGGA GACAAACGCA TTTAAACACC TCACACATCT CTCTCTGAAG 
CTGCTGCCAG GATCTGACAC TGATATTAAG AAGTATCTGG CCAGCTGTTT GTCTTCTGTT 
AAGGAGGAAA AGCAGCAGTT GCAGCAGAAG TTGAGAAAGA CTGAGGAGGA TCTGACAAGA 
CAGCTAAATT ATGCACAGCA AACTCTGTCT GAGAAAAGTC GAGAACTGGA CAAACTTCGG 
TCTGAATGGA CGTCTCAAAC AACCTCTCTG TCCAGCAGAC ACATGCAGGA CCTCACCGCT 
GAGCGGGAAA AAGCTCTGGA GACTCAAAGT CGTCTGCAGC AGCAGAATGA ACAGCTGCGT 
CAGGAACTGG AGTCATCTCA TCACAGAAGC ACCCAACAGC TCCAGACCAA AGTCTCTGAG 
CTGGAGACGG CGAACCGAGA GCTCATCGAC AAGAAATACA AGAGCGACTC CACCATCCGA 
GACCTCAAAG CCAAACTGAC CAGTCTGGAG GAGGAGTGTC AGCGGTCCAA GCAGCAGGTG 
TTGTCGTTGA GGAGGGAGAA CAGCGCTCTG GACAGCGAGT GTCATGAGAA GGAGCGTCTG 
CTGAATCAGC TGCAGACACG AGTGGCCGTT CTGGAGCAGG AGATCAAAGA TAAAGACCAG 
CTTGTGCTGA GGACCAAAGA AGTGCTGGAG GCCACGCAGC AGCAGAAGAA CTCTGTGGAA 
GGAAACGCTG AGAGCAAGCA GCTTCAGATC AGCAAGCTGG AGTCCACAGT GAAGTCTCTG 
TCAGAGGAGC TCATCAAGGC AAACGGCATC ATCAAGAAGT TGCAGGCAGA TCTGAAGGCT 
CTGCTGGGGA AGATCAAGGT GAAGAACAGC GTCACCGTTT CTCAAGAGAA GATCCTGCAG 
GAGACCAGTG ACAAACTGCA GAGACAGCAG AGAGAGCTGC AGGACACACA GCAGAGACTC 
TCACTGAAGG AGGAGGAGGC AGCAAAATTA AAGGAGCAGT TGGAAGCTAC TGTTCAGAAG 
CTGGACGAGA GCAGGGAAGT GTTAAAAACT AATGAGAATG TAATAACGTG GCTGAACAAG 
CAGCTGAATG AGAATCAGCT CTCCAGAAAG CAGGAAACTG TAGCCATGTT TGAGACGCCT 
GCAGCGGCAC TGAGATCAGC CGCTGTCCCG CACAATATGG TCCAGTTTAA CCCGATGAGT 
GTCAAACCTT CAGCAGCAGA GGTGAGTCCA GCTGCTTTTT CCCAGCCGGC CAATAAAGAG 
AACAGTGAAC CTGTAGGATT GGACTCAAAG TACTTTGAGA GACGAGATGA CAGCATTCCT 
CTTCGTGGCC TTCTACCCAG CATGCATCTA AATAGAGAAG TTCCAAAGCC ACTGAACACA 
GCTGCAGCGA AAGCAACACC ATCAGCGTTT TTTCCAGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

In vitro oocyte culture-based manipulation of zebrafish maternal genes.
Developmental dynamics : an official publication of the American Association of Anatomists242(1)44-52(2013 Jan)
Nair S,Lindeman RE,Pelegri F

Insm1a-mediated gene repression is essential for the formation and differentiation of M?ller glia-derived progenitors in the injured retina.
Nature cell biology14(10)1013-23(2012 Oct)
Ramachandran R,Zhao XF,Goldman D

Structures of SAS-6 suggest its organization in centrioles.
Science (New York, N.Y.)331(6021)1196-9(2011 Mar)
van Breugel M,Hirono M,Andreeva A,Yanagisawa HA,Yamaguchi S,Nakazawa Y,Morgner N,Petrovich M,Ebong IO,Robinson CV,Johnson CM,Veprintsev D,Zuber B

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sass6 cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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