pik3cg cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pik3cg
Entrez Gene ID	406598
Full Name	phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
Synonyms	wu:fb15d07,zgc:77033
General protein information	Preferred Names phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma Names phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform phosphoinositide-3-kinase, catalytic, gamma polypeptide
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021474981.1	XP_021330656.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform isoform X1
XM_021474982.1	XP_021330657.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform isoform X2
NM_213306.1	NP_998471.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Conserved IL-2R?c Signaling Mediates Lymphopoiesis in Zebrafish.
Sertori R, Liongue C, Basheer F, Lewis KL, Rasighaemi P, de Coninck D, Traver D, Ward AC
Journal of immunology (Baltimore, Md. : 1950)196(1)135-43(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Kok FO, Shin M, Ni CW, Gupta A, Grosse AS, van Impel A, Kirchmaier BC, Peterson-Maduro J, Kourkoulis G, Male I, DeSantis DF, Sheppard-Tindell S, Ebarasi L, Betsholtz C, Schulte-Merker S, Wolfe SA, Lawson ND
Developmental cell32(1)97-108(2015 Jan)

Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish.
Yoo SK, Deng Q, Cavnar PJ, Wu YI, Hahn KM, Huttenlocher A
Developmental cell18(2)226-36(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pik3cg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pik3cg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141971	XM_021474982.1 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa40881	XM_021474981.1 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa03464	NM_213306.1 Latest version!	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pik3cg ( XM_021474982.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330657.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141971
Clone ID Related Accession (Same CDS sequence)	XM_021474982.1
Accession Version	XM_021474982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACA AAGGGGGAGC CACAGCAGGA TTTAATACAT TGGTTATGGA ACAGCAAGCC 
AGTGATGATG AACCACCAGT AGTTCGCAGA GAGGAAAACA AGAGGAGAAG GAGAAGGATG 
AAAGCATTTA CATCTGCCTC GGCTGTCTCG ATGGAACATA TAGCTGTTGA GTTTGTACTT 
CCTACCACCA ATAAAAATAG CAAAACTTTA GACACATTGC AACTAGACGT GACTGGAAAT 
TGGACGGTAG AGCAGGTGAA GGTTCAGCTT TGGCACAGAG CGGTAACCTC TAAGTTATGC 
CCTGAATTTT ACCAAAAATA CTCTCCAGAC TACTGCATCC TGCTTTATCA GAAGAAAGGC 
AACTGGTATG AAATCTATGA CAAACAGCAG GTATTCCAAA CCCTTGACTG CATTAGATAT 
TGGAAAGCGT TGAGGAAAGA TGTAGGGAAA ATCCATCTGG TTGTTCGGCA ACAACCTGCT 
GAGGATTCGC TTCAGTACCA ACGATTTCTC AACCATCTAA TTGGATATGA TGTCACCGAT 
GTCAGCAACG TCCATGACGA TGAACTGGAG TTTACACGAA GAAAGCTACT GACCCCTCGG 
AAAATTGAGC TATCTGATCG GGATCCCAAC CTTTATTCGA TGGATCCCTG GATGACCACA 
ACACCAATGC CTGAATACTT GCTCAGCAAA ATAAGCAACA ATCATATCCT GATGGTGATA 
CACAAAGAGA CAACAAGCCA GACTATTAAG GTGTCTATAG ATGATACACC AGTCCAGGTA 
CTTCAAAGCT TCTTCGCCAA GATTACTAAC AAGCGAGCAC TTTTAGGCAT ATCTGAAGAC 
GTCAGTGAAT CAGATTTTGT CTTAAGGGTT TGCGGAAGAG AGGAATACCT ATATGGCAAC 
AACGCAATTA AAGATTTCCA CTGGATCAGA CAGTGTCTTA AAAACGGTGA AGAGATTCAT 
CTAGTTTTAG AGCACCCCCC AAACCCAGAA CAAGATGTGG TTCAAAAAGA GGACTGGTCT 
CAGGTTGATG ACTGCACTGG TGTCGCTGGT ACTCACGAAC AACTAACCAT AACTGAGAAA 
GACCATGAAA AGGTGTTCAC CATCTCCCTT TGGGACTGCA ATCGTAAATT CAGGGTGAAG 
ATCTTGGGTA TTGACATTCC AGTACTCCCG CGTAATTCAG AATTGATCGT GTTTGTGGAG 
GCCAGTATTT TCCATGGACA ACAACTGCTG GCTCAAGAAA GGACAAGCTC AAAACCATTC 
ACAGAAGAGG TGATTTGGAA CACCTGGTTG GAGTTTAATA TAAAGATCAA GGACTTACCC 
AAAGGTGCCC GACTTAGCCT GCAAGTGTCG TGTGGAAAGG CACAGACACA AACATCAAAG 
GAAACTGAAT GTAAAAACAA GTCTCGCTTA CTCTACTATG TCAACCTGTT ATTAGTTGAT 
CACCGCTCCC TACTTAGACA AGGTGAGTTC ATCTTGCACA TGTGGAAGAT GCCTGAGAAG 
AGTGAAGACA ACAGCAGTGT AAATGCAGAT AAGCTTACAT CCGCAACCAA CCCAGACAAG 
AACAACTCAA TGGCTGTTGC TATTCTCCTG GACAAGTACT GCTACCCTGT GGCTCTTCCC 
AAGAGCAAAG ATTCTCCTGA TATTGAAGGC GAAAGAGGGC AGAGAGAGAT GCCCAACCAT 
CTACGCAAAC AATTTGAGCA GATTATTGCT ACAGACCCTT TGCACCCGCT CACCTCCGAA 
GATAAAGAGT TGCTCTGGCA CTTCCGGCAG GAGTGCATGA GGGATCCGAA AGCATATCCG 
AAGTTTCTTT CCTCCGTTAA GTGGGGCAAA CAAGAGGCTG TGGCCATAAC GCATTATCTT 
CTGGAAAGGA GCACCATATG GGACCGAAGC GTGCTTGATG TGGGTTTGGT CTTACAATTA 
CTGGACTGCC ACTTCTCTGA TGAAAACGTG CGCACTATAG CTGTCCGGAA GTTGGAGACT 
TTGGGAGATG ATGATGTCCT GCGGTATCTT CTGCAACTTG TCCAGGCTGT AAAATTTGAA 
CCATACCATG ACAGCGCACT TGCAAGGTTC CTTCTGAAAC GTGCACTCAG AAGCAAGCGG 
ATCGGCCATT TCTTGTTCTG GTTTTTAAGA AGTGAAATTG CCCAATCCAT GCACTATCAG 
CAGAGGTATG CTGTAATACT GGAAGCGTAT CTCCGTGGCT GTGGTGAAGA AATGCTACAG 
GACTTCAGGA AGCAGGTGGA GATCACAGAG GCCTTGCAAA AGGTCGCTCG TGAGATTAAG 
CAAATATCTG CTGAGAAGTA TGACATATCA GCACAAGTTG TTTTTCAGCT GCGCCAGAAA 
CTGGAAGGTC TGCAAGTCTT AGGGTTGCCA GACAGCTTTA AAGTGCCTTA CGATCCGGGC 
CTACGAGCAG GGGCACTTGT GATTGAGCAA TGCAAAGTTA TGGCATCCAA GAAGAAACCC 
TTGTGGCTGC AGTTCAAACG AGCCGATCCC ACCACTTTGT CGAGCGACAC AATTGGGATA 
ATCTTTAAAG ATGGGGATGA CCTTCGTCAG GACATGCTGA TATTACAGAT TCTACTTATT 
ATGGAGTCTA TCTGGGAGCT GGAGTCATTA GATCTTTCCT TGTTACCTTA CGGCTGCATT 
TCCACTGGAA ATAAAATTGG AATGATTGAA ATAGTGAAGG ATGCCACTAC CATTGCAAAC 
ATTCAGCAAA GCACTGTTGG GAACACCGGA GCTTTTAAAG ATGAAATCCT CAGCCAGTGG 
CTTCGAGAAA AGTGTGTCAA TGAGGACAAG CATCAACAGG CTGTAGATCG CTTTGTGTTC 
TCGTGTGGAG GGTATTGTGT GGCGACTTAT GTCCTTGGCA TTGGTGATCG TCACAATGAC 
AACATAATGA TCACTGAGAC AGGTAATCTC TTCCACATTG ATTTCGGACA CATACTTGGC 
AACTACAAGA GTTTCTTGGG AATAAGCAAG GAGCGAGTTC CCTTTGTGCT GACTCCAGAT 
TTCCTCTACC TGATGGGCAC AACAGGAAAG AAAAGCAGCC CTAATTTCCT CCAGTTTCAG 
AATGTCTGTC TGAAAGCCTA CTATTTAACA ATATATCTAT GTGGAGGAGG TGCGTGTGGC 
TTTGCCCAGA ATATCCTGAG TTATAGGACG CATCCTCAAG TGCTTATCAT AGCTTATTGT 
TTTCCTGTTC ATACTTGGTT TCGTTATTGT CGTTATTACA TCACTATCAT AACATCACGT 
CATGTGGATC TTGCGCTATG CTGCAAATTT TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330657.1
CDS	37..3312
Translation	MTDKGGATAGFNTLVMEQQASDDEPPVVRREENKRRRRRMKAFTSASAVSMEHIAVEFVLPTTNKNSKTLDTLQLDVTGNWTVEQVKVQLWHRAVTSKLCPEFYQKYSPDYCILLYQKKGNWYEIYDKQQVFQTLDCIRYWKALRKDVGKIHLVVRQQPAEDSLQYQRFLNHLIGYDVTDVSNVHDDELEFTRRKLLTPRKIELSDRDPNLYSMDPWMTTTPMPEYLLSKISNNHILMVIHKETTSQTIKVSIDDTPVQVLQSFFAKITNKRALLGISEDVSESDFVLRVCGREEYLYGNNAIKDFHWIRQCLKNGEEIHLVLEHPPNPEQDVVQKEDWSQVDDCTGVAGTHEQLTITEKDHEKVFTISLWDCNRKFRVKILGIDIPVLPRNSELIVFVEASIFHGQQLLAQERTSSKPFTEEVIWNTWLEFNIKIKDLPKGARLSLQVSCGKAQTQTSKETECKNKSRLLYYVNLLLVDHRSLLRQGEFILHMWKMPEKSEDNSSVNADKLTSATNPDKNNSMAVAILLDKYCYPVALPKSKDSPDIEGERGQREMPNHLRKQFEQIIATDPLHPLTSEDKELLWHFRQECMRDPKAYPKFLSSVKWGKQEAVAITHYLLERSTIWDRSVLDVGLVLQLLDCHFSDENVRTIAVRKLETLGDDDVLRYLLQLVQAVKFEPYHDSALARFLLKRALRSKRIGHFLFWFLRSEIAQSMHYQQRYAVILEAYLRGCGEEMLQDFRKQVEITEALQKVAREIKQISAEKYDISAQVVFQLRQKLEGLQVLGLPDSFKVPYDPGLRAGALVIEQCKVMASKKKPLWLQFKRADPTTLSSDTIGIIFKDGDDLRQDMLILQILLIMESIWELESLDLSLLPYGCISTGNKIGMIEIVKDATTIANIQQSTVGNTGAFKDEILSQWLREKCVNEDKHQQAVDRFVFSCGGYCVATYVLGIGDRHNDNIMITETGNLFHIDFGHILGNYKSFLGISKERVPFVLTPDFLYLMGTTGKKSSPNFLQFQNVCLKAYYLTIYLCGGGACGFAQNILSYRTHPQVLIIAYCFPVHTWFRYCRYYITIITSRHVDLALCCKFL

Target ORF information:

RefSeq Version	XM_021474982.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474982.1

ORF Insert Sequence:

ATGACTGACA AAGGGGGAGC CACAGCAGGA TTTAATACAT TGGTTATGGA ACAGCAAGCC 
AGTGATGATG AACCACCAGT AGTTCGCAGA GAGGAAAACA AGAGGAGAAG GAGAAGGATG 
AAAGCATTTA CATCTGCCTC GGCTGTCTCG ATGGAACATA TAGCTGTTGA GTTTGTACTT 
CCTACCACCA ATAAAAATAG CAAAACTTTA GACACATTGC AACTAGACGT GACTGGAAAT 
TGGACGGTAG AGCAGGTGAA GGTTCAGCTT TGGCACAGAG CGGTAACCTC TAAGTTATGC 
CCTGAATTTT ACCAAAAATA CTCTCCAGAC TACTGCATCC TGCTTTATCA GAAGAAAGGC 
AACTGGTATG AAATCTATGA CAAACAGCAG GTATTCCAAA CCCTTGACTG CATTAGATAT 
TGGAAAGCGT TGAGGAAAGA TGTAGGGAAA ATCCATCTGG TTGTTCGGCA ACAACCTGCT 
GAGGATTCGC TTCAGTACCA ACGATTTCTC AACCATCTAA TTGGATATGA TGTCACCGAT 
GTCAGCAACG TCCATGACGA TGAACTGGAG TTTACACGAA GAAAGCTACT GACCCCTCGG 
AAAATTGAGC TATCTGATCG GGATCCCAAC CTTTATTCGA TGGATCCCTG GATGACCACA 
ACACCAATGC CTGAATACTT GCTCAGCAAA ATAAGCAACA ATCATATCCT GATGGTGATA 
CACAAAGAGA CAACAAGCCA GACTATTAAG GTGTCTATAG ATGATACACC AGTCCAGGTA 
CTTCAAAGCT TCTTCGCCAA GATTACTAAC AAGCGAGCAC TTTTAGGCAT ATCTGAAGAC 
GTCAGTGAAT CAGATTTTGT CTTAAGGGTT TGCGGAAGAG AGGAATACCT ATATGGCAAC 
AACGCAATTA AAGATTTCCA CTGGATCAGA CAGTGTCTTA AAAACGGTGA AGAGATTCAT 
CTAGTTTTAG AGCACCCCCC AAACCCAGAA CAAGATGTGG TTCAAAAAGA GGACTGGTCT 
CAGGTTGATG ACTGCACTGG TGTCGCTGGT ACTCACGAAC AACTAACCAT AACTGAGAAA 
GACCATGAAA AGGTGTTCAC CATCTCCCTT TGGGACTGCA ATCGTAAATT CAGGGTGAAG 
ATCTTGGGTA TTGACATTCC AGTACTCCCG CGTAATTCAG AATTGATCGT GTTTGTGGAG 
GCCAGTATTT TCCATGGACA ACAACTGCTG GCTCAAGAAA GGACAAGCTC AAAACCATTC 
ACAGAAGAGG TGATTTGGAA CACCTGGTTG GAGTTTAATA TAAAGATCAA GGACTTACCC 
AAAGGTGCCC GACTTAGCCT GCAAGTGTCG TGTGGAAAGG CACAGACACA AACATCAAAG 
GAAACTGAAT GTAAAAACAA GTCTCGCTTA CTCTACTATG TCAACCTGTT ATTAGTTGAT 
CACCGCTCCC TACTTAGACA AGGTGAGTTC ATCTTGCACA TGTGGAAGAT GCCTGAGAAG 
AGTGAAGACA ACAGCAGTGT AAATGCAGAT AAGCTTACAT CCGCAACCAA CCCAGACAAG 
AACAACTCAA TGGCTGTTGC TATTCTCCTG GACAAGTACT GCTACCCTGT GGCTCTTCCC 
AAGAGCAAAG ATTCTCCTGA TATTGAAGGC GAAAGAGGGC AGAGAGAGAT GCCCAACCAT 
CTACGCAAAC AATTTGAGCA GATTATTGCT ACAGACCCTT TGCACCCGCT CACCTCCGAA 
GATAAAGAGT TGCTCTGGCA CTTCCGGCAG GAGTGCATGA GGGATCCGAA AGCATATCCG 
AAGTTTCTTT CCTCCGTTAA GTGGGGCAAA CAAGAGGCTG TGGCCATAAC GCATTATCTT 
CTGGAAAGGA GCACCATATG GGACCGAAGC GTGCTTGATG TGGGTTTGGT CTTACAATTA 
CTGGACTGCC ACTTCTCTGA TGAAAACGTG CGCACTATAG CTGTCCGGAA GTTGGAGACT 
TTGGGAGATG ATGATGTCCT GCGGTATCTT CTGCAACTTG TCCAGGCTGT AAAATTTGAA 
CCATACCATG ACAGCGCACT TGCAAGGTTC CTTCTGAAAC GTGCACTCAG AAGCAAGCGG 
ATCGGCCATT TCTTGTTCTG GTTTTTAAGA AGTGAAATTG CCCAATCCAT GCACTATCAG 
CAGAGGTATG CTGTAATACT GGAAGCGTAT CTCCGTGGCT GTGGTGAAGA AATGCTACAG 
GACTTCAGGA AGCAGGTGGA GATCACAGAG GCCTTGCAAA AGGTCGCTCG TGAGATTAAG 
CAAATATCTG CTGAGAAGTA TGACATATCA GCACAAGTTG TTTTTCAGCT GCGCCAGAAA 
CTGGAAGGTC TGCAAGTCTT AGGGTTGCCA GACAGCTTTA AAGTGCCTTA CGATCCGGGC 
CTACGAGCAG GGGCACTTGT GATTGAGCAA TGCAAAGTTA TGGCATCCAA GAAGAAACCC 
TTGTGGCTGC AGTTCAAACG AGCCGATCCC ACCACTTTGT CGAGCGACAC AATTGGGATA 
ATCTTTAAAG ATGGGGATGA CCTTCGTCAG GACATGCTGA TATTACAGAT TCTACTTATT 
ATGGAGTCTA TCTGGGAGCT GGAGTCATTA GATCTTTCCT TGTTACCTTA CGGCTGCATT 
TCCACTGGAA ATAAAATTGG AATGATTGAA ATAGTGAAGG ATGCCACTAC CATTGCAAAC 
ATTCAGCAAA GCACTGTTGG GAACACCGGA GCTTTTAAAG ATGAAATCCT CAGCCAGTGG 
CTTCGAGAAA AGTGTGTCAA TGAGGACAAG CATCAACAGG CTGTAGATCG CTTTGTGTTC 
TCGTGTGGAG GGTATTGTGT GGCGACTTAT GTCCTTGGCA TTGGTGATCG TCACAATGAC 
AACATAATGA TCACTGAGAC AGGTAATCTC TTCCACATTG ATTTCGGACA CATACTTGGC 
AACTACAAGA GTTTCTTGGG AATAAGCAAG GAGCGAGTTC CCTTTGTGCT GACTCCAGAT 
TTCCTCTACC TGATGGGCAC AACAGGAAAG AAAAGCAGCC CTAATTTCCT CCAGTTTCAG 
AATGTCTGTC TGAAAGCCTA CTATTTAACA ATATATCTAT GTGGAGGAGG TGCGTGTGGC 
TTTGCCCAGA ATATCCTGAG TTATAGGACG CATCCTCAAG TGCTTATCAT AGCTTATTGT 
TTTCCTGTTC ATACTTGGTT TCGTTATTGT CGTTATTACA TCACTATCAT AACATCACGT 
CATGTGGATC TTGCGCTATG CTGCAAATTT TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3cg ( XM_021474981.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330656.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40881
Clone ID Related Accession (Same CDS sequence)	XM_021474981.1
Accession Version	XM_021474981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGACA AAGGGGGAGC CACAGCAGGA TTTAATACAT TGGTTATGGA ACAGCAAGCC 
AGTGATGATG AACCACCAGT AGTTCGCAGA GAGGAAAACA AGAGGAGAAG GAGAAGGATG 
AAAGCATTTA CATCTGCCTC GGCTGTCTCG ATGGAACATA TAGCTGTTGA GTTTGTACTT 
CCTACCACCA ATAAAAATAG CAAAACTTTA GACACATTGC AACTAGACGT GACTGGAAAT 
TGGACGGTAG AGCAGGTGAA GGTTCAGCTT TGGCACAGAG CGGTAACCTC TAAGTTATGC 
CCTGAATTTT ACCAAAAATA CTCTCCAGAC TACTGCATCC TGCTTTATCA GAAGAAAGGC 
AACTGGTATG AAATCTATGA CAAACAGCAG GTATTCCAAA CCCTTGACTG CATTAGATAT 
TGGAAAGCGT TGAGGAAAGA TGTAGGGAAA ATCCATCTGG TTGTTCGGCA ACAACCTGCT 
GAGGATTCGC TTCAGTACCA ACGATTTCTC AACCATCTAA TTGGATATGA TGTCACCGAT 
GTCAGCAACG TCCATGACGA TGAACTGGAG TTTACACGAA GAAAGCTACT GACCCCTCGG 
AAAATTGAGC TATCTGATCG GGATCCCAAC CTTTATTCGA TGGATCCCTG GATGACCACA 
ACACCAATGC CTGAATACTT GCTCAGCAAA ATAAGCAACA ATCATATCCT GATGGTGATA 
CACAAAGAGA CAACAAGCCA GACTATTAAG GTGTCTATAG ATGATACACC AGTCCAGGTA 
CTTCAAAGCT TCTTCGCCAA GATTACTAAC AAGCGAGCAC TTTTAGGCAT ATCTGAAGAC 
GTCAGTGAAT CAGATTTTGT CTTAAGGGTT TGCGGAAGAG AGGAATACCT ATATGGCAAC 
AACGCAATTA AAGATTTCCA CTGGATCAGA CAGTGTCTTA AAAACGGTGA AGAGATTCAT 
CTAGTTTTAG AGCACCCCCC AAACCCAGAA CAAGATGTGG TTCAAAAAGA GGACTGGTCT 
CAGGTTGATG ACTGCACTGG TGTCGCTGGT ACTCACGAAC AACTAACCAT AACTGAGAAA 
GACCATGAAA AGGTGTTCAC CATCTCCCTT TGGGACTGCA ATCGTAAATT CAGGGTGAAG 
ATCTTGGGTA TTGACATTCC AGTACTCCCG CGTAATTCAG AATTGATCGT GTTTGTGGAG 
GCCAGTATTT TCCATGGACA ACAACTGCTG GCTCAAGAAA GGACAAGCTC AAAACCATTC 
ACAGAAGAGG TGATTTGGAA CACCTGGTTG GAGTTTAATA TAAAGATCAA GGACTTACCC 
AAAGGTGCCC GACTTAGCCT GCAAGTGTCG TGTGGAAAGG CACAGACACA AACATCAAAG 
GAAACTGAAT GTAAAAACAA GTCTCGCTTA CTCTACTATG TCAACCTGTT ATTAGTTGAT 
CACCGCTCCC TACTTAGACA AGGTGAGTTC ATCTTGCACA TGTGGAAGAT GCCTGAGAAG 
AGTGAAGACA ACAGCAGTGT AAATGCAGAT AAGCTTACAT CCGCAACCAA CCCAGACAAG 
AACAACTCAA TGGCTGTTGC TATTCTCCTG GACAAGTACT GCTACCCTGT GGCTCTTCCC 
AAGAGCAAAG ATTCTCCTGA TATTGAAGGC GAAAGAGGGC AGAGAGAGAT GCCCAACCAT 
CTACGCAAAC AATTTGAGCA GATTATTGCT ACAGACCCTT TGCACCCGCT CACCTCCGAA 
GATAAAGAGT TGCTCTGGCA CTTCCGGCAG GAGTGCATGA GGGATCCGAA AGCATATCCG 
AAGTTTCTTT CCTCCGTTAA GTGGGGCAAA CAAGAGGCTG TGGCCATAAC GCATTATCTT 
CTGGAAAGGA GCACCATATG GGACCGAAGC GTGCTTGATG TGGGTTTGGT CTTACAATTA 
CTGGACTGCC ACTTCTCTGA TGAAAACGTG CGCACTATAG CTGTCCGGAA GTTGGAGACT 
TTGGGAGATG ATGATGTCCT GCGGTATCTT CTGCAACTTG TCCAGGCTGT AAAATTTGAA 
CCATACCATG ACAGCGCACT TGCAAGGTTC CTTCTGAAAC GTGCACTCAG AAGCAAGCGG 
ATCGGCCATT TCTTGTTCTG GTTTTTAAGA AGTGAAATTG CCCAATCCAT GCACTATCAG 
CAGAGGTATG CTGTAATACT GGAAGCGTAT CTCCGTGGCT GTGGTGAAGA AATGCTACAG 
GACTTCAGGA AGCAGGTGGA GATCACAGAG GCCTTGCAAA AGGTCGCTCG TGAGATTAAG 
CAAATATCTG CTGAGAAGTA TGACATATCA GCACAAGTTG TTTTTCAGCT GCGCCAGAAA 
CTGGAAGGTC TGCAAGTCTT AGGGTTGCCA GACAGCTTTA AAGTGCCTTA CGATCCGGGC 
CTACGAGCAG GGGCACTTGT GATTGAGCAA TGCAAAGTTA TGGCATCCAA GAAGAAACCC 
TTGTGGCTGC AGTTCAAACG AGCCGATCCC ACCACTTTGT CGAGCGACAC AATTGGGATA 
ATCTTTAAAG ATGGGGATGA CCTTCGTCAG GACATGCTGA TATTACAGAT TCTACTTATT 
ATGGAGTCTA TCTGGGAGCT GGAGTCATTA GATCTTTCCT TGTTACCTTA CGGCTGCATT 
TCCACTGGAA ATAAAATTGG AATGATTGAA ATAGTGAAGG ATGCCACTAC CATTGCAAAC 
ATTCAGCAAA GCACTGTTGG GAACACCGGA GCTTTTAAAG ATGAAATCCT CAGCCAGTGG 
CTTCGAGAAA AGTGTGTCAA TGAGGACAAG CATCAACAGG CTGTAGATCG CTTTGTGTTC 
TCGTGTGGAG GGTATTGTGT GGCGACTTAT GTCCTTGGCA TTGGTGATCG TCACAATGAC 
AACATAATGA TCACTGAGAC AGGTAATCTC TTCCACATTG ATTTCGGACA CATACTTGGC 
AACTACAAGA GTTTCTTGGG AATAAGCAAG GAGCGAGTTC CCTTTGTGCT GACTCCAGAT 
TTCCTCTACC TGATGGGCAC AACAGGAAAG AAAAGCAGCC CTAATTTCCT CCAGTTTCAG 
AATGTCTGTC TGAAGGCCTA CCTGGCGCTG AGACACCACA CCAACCTGCT GATCATCCTG 
TTCTCCATGA TGCTGATGAC CGGGATGCCT CAACTCACCA GCAAAGAGGA CATAGAGTAC 
ATTCGGGAGG CGCTGACAGT AGGACGCTCA GAGGACGAGG CCAAACAGCA CTTCTTGGAC 
CAAATTGAGA TCTGTCGGGA CAAGGGTTGG ACGGTGCAGT TTAACTGGTT CCTACATTTG 
GTGCTCGGCA TTAAACAGGG TGTGGAGAAA CGGTCAGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330656.1
CDS	35..3376
Translation	MTDKGGATAGFNTLVMEQQASDDEPPVVRREENKRRRRRMKAFTSASAVSMEHIAVEFVLPTTNKNSKTLDTLQLDVTGNWTVEQVKVQLWHRAVTSKLCPEFYQKYSPDYCILLYQKKGNWYEIYDKQQVFQTLDCIRYWKALRKDVGKIHLVVRQQPAEDSLQYQRFLNHLIGYDVTDVSNVHDDELEFTRRKLLTPRKIELSDRDPNLYSMDPWMTTTPMPEYLLSKISNNHILMVIHKETTSQTIKVSIDDTPVQVLQSFFAKITNKRALLGISEDVSESDFVLRVCGREEYLYGNNAIKDFHWIRQCLKNGEEIHLVLEHPPNPEQDVVQKEDWSQVDDCTGVAGTHEQLTITEKDHEKVFTISLWDCNRKFRVKILGIDIPVLPRNSELIVFVEASIFHGQQLLAQERTSSKPFTEEVIWNTWLEFNIKIKDLPKGARLSLQVSCGKAQTQTSKETECKNKSRLLYYVNLLLVDHRSLLRQGEFILHMWKMPEKSEDNSSVNADKLTSATNPDKNNSMAVAILLDKYCYPVALPKSKDSPDIEGERGQREMPNHLRKQFEQIIATDPLHPLTSEDKELLWHFRQECMRDPKAYPKFLSSVKWGKQEAVAITHYLLERSTIWDRSVLDVGLVLQLLDCHFSDENVRTIAVRKLETLGDDDVLRYLLQLVQAVKFEPYHDSALARFLLKRALRSKRIGHFLFWFLRSEIAQSMHYQQRYAVILEAYLRGCGEEMLQDFRKQVEITEALQKVAREIKQISAEKYDISAQVVFQLRQKLEGLQVLGLPDSFKVPYDPGLRAGALVIEQCKVMASKKKPLWLQFKRADPTTLSSDTIGIIFKDGDDLRQDMLILQILLIMESIWELESLDLSLLPYGCISTGNKIGMIEIVKDATTIANIQQSTVGNTGAFKDEILSQWLREKCVNEDKHQQAVDRFVFSCGGYCVATYVLGIGDRHNDNIMITETGNLFHIDFGHILGNYKSFLGISKERVPFVLTPDFLYLMGTTGKKSSPNFLQFQNVCLKAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEYIREALTVGRSEDEAKQHFLDQIEICRDKGWTVQFNWFLHLVLGIKQGVEKRSA

Target ORF information:

RefSeq Version	XM_021474981.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474981.1

ORF Insert Sequence:

ATGACTGACA AAGGGGGAGC CACAGCAGGA TTTAATACAT TGGTTATGGA ACAGCAAGCC 
AGTGATGATG AACCACCAGT AGTTCGCAGA GAGGAAAACA AGAGGAGAAG GAGAAGGATG 
AAAGCATTTA CATCTGCCTC GGCTGTCTCG ATGGAACATA TAGCTGTTGA GTTTGTACTT 
CCTACCACCA ATAAAAATAG CAAAACTTTA GACACATTGC AACTAGACGT GACTGGAAAT 
TGGACGGTAG AGCAGGTGAA GGTTCAGCTT TGGCACAGAG CGGTAACCTC TAAGTTATGC 
CCTGAATTTT ACCAAAAATA CTCTCCAGAC TACTGCATCC TGCTTTATCA GAAGAAAGGC 
AACTGGTATG AAATCTATGA CAAACAGCAG GTATTCCAAA CCCTTGACTG CATTAGATAT 
TGGAAAGCGT TGAGGAAAGA TGTAGGGAAA ATCCATCTGG TTGTTCGGCA ACAACCTGCT 
GAGGATTCGC TTCAGTACCA ACGATTTCTC AACCATCTAA TTGGATATGA TGTCACCGAT 
GTCAGCAACG TCCATGACGA TGAACTGGAG TTTACACGAA GAAAGCTACT GACCCCTCGG 
AAAATTGAGC TATCTGATCG GGATCCCAAC CTTTATTCGA TGGATCCCTG GATGACCACA 
ACACCAATGC CTGAATACTT GCTCAGCAAA ATAAGCAACA ATCATATCCT GATGGTGATA 
CACAAAGAGA CAACAAGCCA GACTATTAAG GTGTCTATAG ATGATACACC AGTCCAGGTA 
CTTCAAAGCT TCTTCGCCAA GATTACTAAC AAGCGAGCAC TTTTAGGCAT ATCTGAAGAC 
GTCAGTGAAT CAGATTTTGT CTTAAGGGTT TGCGGAAGAG AGGAATACCT ATATGGCAAC 
AACGCAATTA AAGATTTCCA CTGGATCAGA CAGTGTCTTA AAAACGGTGA AGAGATTCAT 
CTAGTTTTAG AGCACCCCCC AAACCCAGAA CAAGATGTGG TTCAAAAAGA GGACTGGTCT 
CAGGTTGATG ACTGCACTGG TGTCGCTGGT ACTCACGAAC AACTAACCAT AACTGAGAAA 
GACCATGAAA AGGTGTTCAC CATCTCCCTT TGGGACTGCA ATCGTAAATT CAGGGTGAAG 
ATCTTGGGTA TTGACATTCC AGTACTCCCG CGTAATTCAG AATTGATCGT GTTTGTGGAG 
GCCAGTATTT TCCATGGACA ACAACTGCTG GCTCAAGAAA GGACAAGCTC AAAACCATTC 
ACAGAAGAGG TGATTTGGAA CACCTGGTTG GAGTTTAATA TAAAGATCAA GGACTTACCC 
AAAGGTGCCC GACTTAGCCT GCAAGTGTCG TGTGGAAAGG CACAGACACA AACATCAAAG 
GAAACTGAAT GTAAAAACAA GTCTCGCTTA CTCTACTATG TCAACCTGTT ATTAGTTGAT 
CACCGCTCCC TACTTAGACA AGGTGAGTTC ATCTTGCACA TGTGGAAGAT GCCTGAGAAG 
AGTGAAGACA ACAGCAGTGT AAATGCAGAT AAGCTTACAT CCGCAACCAA CCCAGACAAG 
AACAACTCAA TGGCTGTTGC TATTCTCCTG GACAAGTACT GCTACCCTGT GGCTCTTCCC 
AAGAGCAAAG ATTCTCCTGA TATTGAAGGC GAAAGAGGGC AGAGAGAGAT GCCCAACCAT 
CTACGCAAAC AATTTGAGCA GATTATTGCT ACAGACCCTT TGCACCCGCT CACCTCCGAA 
GATAAAGAGT TGCTCTGGCA CTTCCGGCAG GAGTGCATGA GGGATCCGAA AGCATATCCG 
AAGTTTCTTT CCTCCGTTAA GTGGGGCAAA CAAGAGGCTG TGGCCATAAC GCATTATCTT 
CTGGAAAGGA GCACCATATG GGACCGAAGC GTGCTTGATG TGGGTTTGGT CTTACAATTA 
CTGGACTGCC ACTTCTCTGA TGAAAACGTG CGCACTATAG CTGTCCGGAA GTTGGAGACT 
TTGGGAGATG ATGATGTCCT GCGGTATCTT CTGCAACTTG TCCAGGCTGT AAAATTTGAA 
CCATACCATG ACAGCGCACT TGCAAGGTTC CTTCTGAAAC GTGCACTCAG AAGCAAGCGG 
ATCGGCCATT TCTTGTTCTG GTTTTTAAGA AGTGAAATTG CCCAATCCAT GCACTATCAG 
CAGAGGTATG CTGTAATACT GGAAGCGTAT CTCCGTGGCT GTGGTGAAGA AATGCTACAG 
GACTTCAGGA AGCAGGTGGA GATCACAGAG GCCTTGCAAA AGGTCGCTCG TGAGATTAAG 
CAAATATCTG CTGAGAAGTA TGACATATCA GCACAAGTTG TTTTTCAGCT GCGCCAGAAA 
CTGGAAGGTC TGCAAGTCTT AGGGTTGCCA GACAGCTTTA AAGTGCCTTA CGATCCGGGC 
CTACGAGCAG GGGCACTTGT GATTGAGCAA TGCAAAGTTA TGGCATCCAA GAAGAAACCC 
TTGTGGCTGC AGTTCAAACG AGCCGATCCC ACCACTTTGT CGAGCGACAC AATTGGGATA 
ATCTTTAAAG ATGGGGATGA CCTTCGTCAG GACATGCTGA TATTACAGAT TCTACTTATT 
ATGGAGTCTA TCTGGGAGCT GGAGTCATTA GATCTTTCCT TGTTACCTTA CGGCTGCATT 
TCCACTGGAA ATAAAATTGG AATGATTGAA ATAGTGAAGG ATGCCACTAC CATTGCAAAC 
ATTCAGCAAA GCACTGTTGG GAACACCGGA GCTTTTAAAG ATGAAATCCT CAGCCAGTGG 
CTTCGAGAAA AGTGTGTCAA TGAGGACAAG CATCAACAGG CTGTAGATCG CTTTGTGTTC 
TCGTGTGGAG GGTATTGTGT GGCGACTTAT GTCCTTGGCA TTGGTGATCG TCACAATGAC 
AACATAATGA TCACTGAGAC AGGTAATCTC TTCCACATTG ATTTCGGACA CATACTTGGC 
AACTACAAGA GTTTCTTGGG AATAAGCAAG GAGCGAGTTC CCTTTGTGCT GACTCCAGAT 
TTCCTCTACC TGATGGGCAC AACAGGAAAG AAAAGCAGCC CTAATTTCCT CCAGTTTCAG 
AATGTCTGTC TGAAGGCCTA CCTGGCGCTG AGACACCACA CCAACCTGCT GATCATCCTG 
TTCTCCATGA TGCTGATGAC CGGGATGCCT CAACTCACCA GCAAAGAGGA CATAGAGTAC 
ATTCGGGAGG CGCTGACAGT AGGACGCTCA GAGGACGAGG CCAAACAGCA CTTCTTGGAC 
CAAATTGAGA TCTGTCGGGA CAAGGGTTGG ACGGTGCAGT TTAACTGGTT CCTACATTTG 
GTGCTCGGCA TTAAACAGGG TGTGGAGAAA CGGTCAGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pik3cg ( NM_213306.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998471.1 Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), mRNA.

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CloneID	ODa03464
Clone ID Related Accession (Same CDS sequence)	NM_213306.1
Accession Version	NM_213306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068983.1. ##Evidence-Data-START## Transcript exon combination :: BC068983.1, BC057425.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAACAGC AAGCCAGTGA TGATGAACCA CCAGTAGTTC GCAGAGAGGA AAACAAGAGG 
AGAAGGAGAA GGATGAAAGC ATTTACATCT GCCTCGGCTG TCTCGATGGA ACATATAGCT 
GTTGAGTTTG TACTTCCTAC CACCAATAAA AATAGCAAAA CTTTAGACAC ATTGCAACTA 
GACGTGACTG GAAATTGGAC GGTAGAGCAG GTGAAGGTTC AGCTTTGGCA CAGAGCGGTA 
ACCTCTAAGT TATGCCCTGA ATTTTACCAA AAATACTCTC CAGACTACTG CATCCTGCTT 
TATCAGAAGA AAGGCAACTG GTATGAAATC TATGACAAAC AGCAGGTATT CCAAACCCTT 
GACTGCATTA GATATTGGAA AGCGTTGAGG AAAGATGTAG GGAAAATCCA TCTGGTTGTT 
CGGCAACAAC CTGCTGAGGA TTCGCTTCAG TACCAACGAT TTCTCAACCA TCTAATTGGA 
TATGATGTCA CCGATGTCAG CAACGTCCAT GACGATGAAC TGGAGTTTAC ACGAAGAAAG 
CTACTGACCC CTCGGAAAAT TGAGCTATCT GATCGGGATC CCAACCTTTA TTCGATGGAT 
CCCTGGATGA CCACAACACC CATGCCTGAA TACTTGCTCA GCAAAATAAG CAACAATCAT 
ATCCTTATGG TGATACACAA AGAGACAACA AGCCAGACTA TTAAGGTGTC TATAGATGAT 
ACACCAGTCC AGGTACTTCA AAGCTTCTTC GCCAAGATTA CTAACAAGAG AGCACTTTTA 
GGCATATCGG AAGACGTCAG TGAATCAGAT TTTGTCTTGA GGGTTTGCGG AAGAGAGGAA 
TACCTATATG GCAACAACGC AATTAAAGAT TTCCACTGGA TCAGACAGTG TCTTAAAAAC 
GGTGAAGAGA TTCATCTAGT TTTAGAGCAC CCCCCAAACC CAGAACAAGA TGTGGTTCAA 
AAAGAGGACT GGTCTCAGGT CGATGACTGC ACTGGTGTCG CTGGTACTCA CGAACAACTC 
ACCATAACTG AGAAAGACCA TGAAAAGGTG TTCACCATCT CCCTTTGGGA CTGCAATCGT 
AAATTCAGGG TGAAGATCTT GGGTATTGAC ATCCCAGTAC TCCCGCGTAA TTCAGAATTG 
ATCGTGTTTG TAGAGGCCAG TATTTTCCAT GGACAACAAC TGCTGGCTCA AGAAAGGACA 
AGCTCAAAAC CATTCACAGA AGAGGTGATT TGGAACACCT GGTTGGAGTT TAATATAAAG 
ATCAAGGACT TACCCAAAGG TGCCCGACTT AGCCTGCAAG TGTCGTGTGG AAAGGCACAG 
ACACAAACAT CTAAGGAAAC TGAATGTAAA AACAAGTCTC GCTTACTCTA CTATGTCAAC 
TTGTTATTAG TTGATCACCG CTCCCTACTT AGACAAGGTG AGTTCATCTT GCACATGTGG 
AAGATGCCTG AGAAGAGTGA AGACAACAGC AGTGTAAATG CAGATAAGCT TACATCCGCA 
ACCAACCCAG ACAAGAACAA CTCAATGGCT GTTGCTATTC TCCTGGACAA GTACTGCTAC 
CCTGTGGCTC TTCCCAAGAG CAAAGATTCT CCTGATATTG AAGGTGAAAG AGGGCAGAGA 
GAGATGCCCA ACCATCTACG CAAACAATTT GAGCAGATTA TTGCTACAGA CCCTTTGCAC 
CCGCTCACCT CCGAAGATAA AGAGTTGCTC TGGCACTTCA GGCAGGAGTG CATGAGGGAT 
CCGAAAGCAT ATCCGAAGTT TCTTTCCTCC GTTAAGTGGG GCAAACAAGA GGCTGTGGCC 
ATAACGCATC ATCTTCTGGA AAGGAGCACC ATATGGGACC GAAGCGTGCT TGATGTGGGT 
TTGGTCTTAC AATTACTGGA CTGCCACTTC TCTGATGAAA ACGTGCGCAC TATAGCTGTC 
CGGAAGTTGG AGACTTTGGG AGATGATGAT GTCCTGCGGT ATCTTCTGCA ACTTGTCCAG 
GCTGTAAAAT TTGAACCATA CCATGACAGC GCACTTGCAA GGTTCCTTCT GAAACGTGCA 
CTCAGAAGCA AGCGGATCGG CCATTTCTTG TTTTGGTTTT TAAGAAGTGA AATTGCCCAA 
TCCATGCACT ATCAGCAGAG GTATGCTGTA ATACTGGAAG CGTATCTCCG TGGCTGTGGT 
GAAGAAATGC TACAGGACTT CAGGAAGCAG GTGGAGATCA CAGAGGCCTT GCAAAAGGTC 
GCTCGTGAGA TTAAGCAAAT ATCTGCTGAG AAGTATGACA TATCAGCACA AGTTGTTTTT 
CAGCTGCGCC AGAAACTGGA AGGTCTGCAA GTCTTAGGGT TGCCAGACAG CTTTAAAGTG 
CCTTACGATC CAGGCCTACG AGCAGGGGCA CTTGTGATTG AACAATGCAA AGTTATGGCA 
TCCAAGAAGA AACCCTTGTG GCTGCAGTTC AAACGGGCCG ATCCCACCAC CTTGTCGAGC 
GACACAATTG GGATAATCTT TAAAGATGGG GATGACCTTC GTCAGGACAT GCTGATATTA 
CAGATTCTAC TTATTATGGA GTCTATCTGG GAGCTGGAGT CATTGGATCT TTCCTTGTTA 
CCTTACGGCT GCATTTCCAC TGGAAATAAA ATTGGAATGA TTGAAATAGT GAAGGATGCC 
ACTACCATTG CAAACATTCA GCAAAGCACT GTTGGGAACA CCGGAGCTTT TAAAGATGAA 
ATCCTCAGCC AGTGGCTTCG AGAAAAGTGT GTCAATGAGG ACAAGCATCA ACAGGCTGTA 
GATCGCTTTG TGTTCTCGTG TGGCGGGTAT TGTGTGGCGA CTTATGTCCT TGGCATTGGT 
GATCGTCACA ATGACAACAT AATGATCACT GAGACAGGTA ATCTCTTCCA CATTGATTTC 
GGACACATAC TTGGCAACTA CAAGAGTTTC TTGGGAATAA GCAAGGAGCG AGTTCCCTTT 
GTGCTGACTC CAGATTTCCT CTACCTGATG GGCACAACAG GAAAGAAAAG CAGCCCTAAT 
TTCCTCCAGT TTCAGAATGT CTGTCTGAAG GCCTACCTGG CGCTGAGACA CCACACCAAC 
CTGCTGATCA TCCTGTTCTC CATGATGCTG ATGACCGGGA TGCCTCAACT CACCAGCAAA 
GAGGACATCG AGTACATTCG GGAGGCGCTG ACAGTAGGAC GCTCAGAGGA CGAGGCCAAA 
CAGCACTTCT TGGACCAAAT TGAGATCTGT CGGGACAAGG GTTGGACGGT GCAGTTTAAC 
TGGTTCCTAC ATTTGGTGCT CGGCATTAAA CAGGGTGTGG AGAAACGGTC AGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998471.1
CDS	166..3462
Translation	MEQQASDDEPPVVRREENKRRRRRMKAFTSASAVSMEHIAVEFVLPTTNKNSKTLDTLQLDVTGNWTVEQVKVQLWHRAVTSKLCPEFYQKYSPDYCILLYQKKGNWYEIYDKQQVFQTLDCIRYWKALRKDVGKIHLVVRQQPAEDSLQYQRFLNHLIGYDVTDVSNVHDDELEFTRRKLLTPRKIELSDRDPNLYSMDPWMTTTPMPEYLLSKISNNHILMVIHKETTSQTIKVSIDDTPVQVLQSFFAKITNKRALLGISEDVSESDFVLRVCGREEYLYGNNAIKDFHWIRQCLKNGEEIHLVLEHPPNPEQDVVQKEDWSQVDDCTGVAGTHEQLTITEKDHEKVFTISLWDCNRKFRVKILGIDIPVLPRNSELIVFVEASIFHGQQLLAQERTSSKPFTEEVIWNTWLEFNIKIKDLPKGARLSLQVSCGKAQTQTSKETECKNKSRLLYYVNLLLVDHRSLLRQGEFILHMWKMPEKSEDNSSVNADKLTSATNPDKNNSMAVAILLDKYCYPVALPKSKDSPDIEGERGQREMPNHLRKQFEQIIATDPLHPLTSEDKELLWHFRQECMRDPKAYPKFLSSVKWGKQEAVAITHHLLERSTIWDRSVLDVGLVLQLLDCHFSDENVRTIAVRKLETLGDDDVLRYLLQLVQAVKFEPYHDSALARFLLKRALRSKRIGHFLFWFLRSEIAQSMHYQQRYAVILEAYLRGCGEEMLQDFRKQVEITEALQKVAREIKQISAEKYDISAQVVFQLRQKLEGLQVLGLPDSFKVPYDPGLRAGALVIEQCKVMASKKKPLWLQFKRADPTTLSSDTIGIIFKDGDDLRQDMLILQILLIMESIWELESLDLSLLPYGCISTGNKIGMIEIVKDATTIANIQQSTVGNTGAFKDEILSQWLREKCVNEDKHQQAVDRFVFSCGGYCVATYVLGIGDRHNDNIMITETGNLFHIDFGHILGNYKSFLGISKERVPFVLTPDFLYLMGTTGKKSSPNFLQFQNVCLKAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEYIREALTVGRSEDEAKQHFLDQIEICRDKGWTVQFNWFLHLVLGIKQGVEKRSA

Target ORF information:

RefSeq Version	NM_213306.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma (pik3cg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213306.1

ORF Insert Sequence:

ATGGAACAGC AAGCCAGTGA TGATGAACCA CCAGTAGTTC GCAGAGAGGA AAACAAGAGG 
AGAAGGAGAA GGATGAAAGC ATTTACATCT GCCTCGGCTG TCTCGATGGA ACATATAGCT 
GTTGAGTTTG TACTTCCTAC CACCAATAAA AATAGCAAAA CTTTAGACAC ATTGCAACTA 
GACGTGACTG GAAATTGGAC GGTAGAGCAG GTGAAGGTTC AGCTTTGGCA CAGAGCGGTA 
ACCTCTAAGT TATGCCCTGA ATTTTACCAA AAATACTCTC CAGACTACTG CATCCTGCTT 
TATCAGAAGA AAGGCAACTG GTATGAAATC TATGACAAAC AGCAGGTATT CCAAACCCTT 
GACTGCATTA GATATTGGAA AGCGTTGAGG AAAGATGTAG GGAAAATCCA TCTGGTTGTT 
CGGCAACAAC CTGCTGAGGA TTCGCTTCAG TACCAACGAT TTCTCAACCA TCTAATTGGA 
TATGATGTCA CCGATGTCAG CAACGTCCAT GACGATGAAC TGGAGTTTAC ACGAAGAAAG 
CTACTGACCC CTCGGAAAAT TGAGCTATCT GATCGGGATC CCAACCTTTA TTCGATGGAT 
CCCTGGATGA CCACAACACC CATGCCTGAA TACTTGCTCA GCAAAATAAG CAACAATCAT 
ATCCTTATGG TGATACACAA AGAGACAACA AGCCAGACTA TTAAGGTGTC TATAGATGAT 
ACACCAGTCC AGGTACTTCA AAGCTTCTTC GCCAAGATTA CTAACAAGAG AGCACTTTTA 
GGCATATCGG AAGACGTCAG TGAATCAGAT TTTGTCTTGA GGGTTTGCGG AAGAGAGGAA 
TACCTATATG GCAACAACGC AATTAAAGAT TTCCACTGGA TCAGACAGTG TCTTAAAAAC 
GGTGAAGAGA TTCATCTAGT TTTAGAGCAC CCCCCAAACC CAGAACAAGA TGTGGTTCAA 
AAAGAGGACT GGTCTCAGGT CGATGACTGC ACTGGTGTCG CTGGTACTCA CGAACAACTC 
ACCATAACTG AGAAAGACCA TGAAAAGGTG TTCACCATCT CCCTTTGGGA CTGCAATCGT 
AAATTCAGGG TGAAGATCTT GGGTATTGAC ATCCCAGTAC TCCCGCGTAA TTCAGAATTG 
ATCGTGTTTG TAGAGGCCAG TATTTTCCAT GGACAACAAC TGCTGGCTCA AGAAAGGACA 
AGCTCAAAAC CATTCACAGA AGAGGTGATT TGGAACACCT GGTTGGAGTT TAATATAAAG 
ATCAAGGACT TACCCAAAGG TGCCCGACTT AGCCTGCAAG TGTCGTGTGG AAAGGCACAG 
ACACAAACAT CTAAGGAAAC TGAATGTAAA AACAAGTCTC GCTTACTCTA CTATGTCAAC 
TTGTTATTAG TTGATCACCG CTCCCTACTT AGACAAGGTG AGTTCATCTT GCACATGTGG 
AAGATGCCTG AGAAGAGTGA AGACAACAGC AGTGTAAATG CAGATAAGCT TACATCCGCA 
ACCAACCCAG ACAAGAACAA CTCAATGGCT GTTGCTATTC TCCTGGACAA GTACTGCTAC 
CCTGTGGCTC TTCCCAAGAG CAAAGATTCT CCTGATATTG AAGGTGAAAG AGGGCAGAGA 
GAGATGCCCA ACCATCTACG CAAACAATTT GAGCAGATTA TTGCTACAGA CCCTTTGCAC 
CCGCTCACCT CCGAAGATAA AGAGTTGCTC TGGCACTTCA GGCAGGAGTG CATGAGGGAT 
CCGAAAGCAT ATCCGAAGTT TCTTTCCTCC GTTAAGTGGG GCAAACAAGA GGCTGTGGCC 
ATAACGCATC ATCTTCTGGA AAGGAGCACC ATATGGGACC GAAGCGTGCT TGATGTGGGT 
TTGGTCTTAC AATTACTGGA CTGCCACTTC TCTGATGAAA ACGTGCGCAC TATAGCTGTC 
CGGAAGTTGG AGACTTTGGG AGATGATGAT GTCCTGCGGT ATCTTCTGCA ACTTGTCCAG 
GCTGTAAAAT TTGAACCATA CCATGACAGC GCACTTGCAA GGTTCCTTCT GAAACGTGCA 
CTCAGAAGCA AGCGGATCGG CCATTTCTTG TTTTGGTTTT TAAGAAGTGA AATTGCCCAA 
TCCATGCACT ATCAGCAGAG GTATGCTGTA ATACTGGAAG CGTATCTCCG TGGCTGTGGT 
GAAGAAATGC TACAGGACTT CAGGAAGCAG GTGGAGATCA CAGAGGCCTT GCAAAAGGTC 
GCTCGTGAGA TTAAGCAAAT ATCTGCTGAG AAGTATGACA TATCAGCACA AGTTGTTTTT 
CAGCTGCGCC AGAAACTGGA AGGTCTGCAA GTCTTAGGGT TGCCAGACAG CTTTAAAGTG 
CCTTACGATC CAGGCCTACG AGCAGGGGCA CTTGTGATTG AACAATGCAA AGTTATGGCA 
TCCAAGAAGA AACCCTTGTG GCTGCAGTTC AAACGGGCCG ATCCCACCAC CTTGTCGAGC 
GACACAATTG GGATAATCTT TAAAGATGGG GATGACCTTC GTCAGGACAT GCTGATATTA 
CAGATTCTAC TTATTATGGA GTCTATCTGG GAGCTGGAGT CATTGGATCT TTCCTTGTTA 
CCTTACGGCT GCATTTCCAC TGGAAATAAA ATTGGAATGA TTGAAATAGT GAAGGATGCC 
ACTACCATTG CAAACATTCA GCAAAGCACT GTTGGGAACA CCGGAGCTTT TAAAGATGAA 
ATCCTCAGCC AGTGGCTTCG AGAAAAGTGT GTCAATGAGG ACAAGCATCA ACAGGCTGTA 
GATCGCTTTG TGTTCTCGTG TGGCGGGTAT TGTGTGGCGA CTTATGTCCT TGGCATTGGT 
GATCGTCACA ATGACAACAT AATGATCACT GAGACAGGTA ATCTCTTCCA CATTGATTTC 
GGACACATAC TTGGCAACTA CAAGAGTTTC TTGGGAATAA GCAAGGAGCG AGTTCCCTTT 
GTGCTGACTC CAGATTTCCT CTACCTGATG GGCACAACAG GAAAGAAAAG CAGCCCTAAT 
TTCCTCCAGT TTCAGAATGT CTGTCTGAAG GCCTACCTGG CGCTGAGACA CCACACCAAC 
CTGCTGATCA TCCTGTTCTC CATGATGCTG ATGACCGGGA TGCCTCAACT CACCAGCAAA 
GAGGACATCG AGTACATTCG GGAGGCGCTG ACAGTAGGAC GCTCAGAGGA CGAGGCCAAA 
CAGCACTTCT TGGACCAAAT TGAGATCTGT CGGGACAAGG GTTGGACGGT GCAGTTTAAC 
TGGTTCCTAC ATTTGGTGCT CGGCATTAAA CAGGGTGTGG AGAAACGGTC AGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Conserved IL-2R?c Signaling Mediates Lymphopoiesis in Zebrafish.
Journal of immunology (Baltimore, Md. : 1950)196(1)135-43(2016 Jan)
Sertori R,Liongue C,Basheer F,Lewis KL,Rasighaemi P,de Coninck D,Traver D,Ward AC

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Developmental cell32(1)97-108(2015 Jan)
Kok FO,Shin M,Ni CW,Gupta A,Grosse AS,van Impel A,Kirchmaier BC,Peterson-Maduro J,Kourkoulis G,Male I,DeSantis DF,Sheppard-Tindell S,Ebarasi L,Betsholtz C,Schulte-Merker S,Wolfe SA,Lawson ND

Differential regulation of protrusion and polarity by PI3K during neutrophil motility in live zebrafish.
Developmental cell18(2)226-36(2010 Feb)
Yoo SK,Deng Q,Cavnar PJ,Wu YI,Hahn KM,Huttenlocher A

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pik3cg cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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