ak4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ak4
Entrez Gene ID	406590
Full Name	adenylate kinase 4
Synonyms	ak3l1,wu:fc37g02,zgc:85790
General protein information	Preferred Names adenylate kinase 4 Names adenylate kinase 4, mitochondrial adenylate kinase 3-like 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165873.3	XP_005165930.1	adenylate kinase 4, mitochondrial isoform X1
XM_005165871.3	XP_005165928.1	adenylate kinase 4, mitochondrial isoform X1
NM_213299.1	NP_998464.1	adenylate kinase 4, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AK4	XP_003949482.1
Rattus norvegicus (Norway rat)	Ak4	NP_058831.1
Canis lupus familiaris (dog)	AK4	XP_546674.2
Mus musculus (house mouse)	Ak4	NP_001171073.1
Gallus gallus (chicken)	AK4	XP_422526.3
	AK4	XP_001089356.1
Bos taurus (cattle)	AK4	NP_001071401.1
Homo sapiens (human)	AK4	NP_001005353.1
Danio rerio (zebrafish)	ak4	NP_998464.1
Xenopus tropicalis (tropical clawed frog)	ak4	XP_002931643.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Xmrk, kras and myc transgenic zebrafish liver cancer models share molecular signatures with subsets of human hepatocellular carcinoma.
Zheng W, Li Z, Nguyen AT, Li C, Emelyanov A, Gong Z
PloS one9(3)e91179(2014)

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Baraban SC, Dinday MT, Hortopan GA
Nature communications42410(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ak4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ak4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00733	NM_213299.1 Latest version!	Danio rerio adenylate kinase 4 (ak4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa11914	XM_005165871.3 Latest version!	Danio rerio adenylate kinase 4 (ak4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa11914	XM_005165873.3 Latest version!	Danio rerio adenylate kinase 4 (ak4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ak4 ( NM_213299.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998464.1 Danio rerio adenylate kinase 4 (ak4), mRNA.

Price & Availability↑

CloneID	ODa00733
Clone ID Related Accession (Same CDS sequence)	NM_213299.1
Accession Version	NM_213299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	adenylate kinase 4, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068387.1. ##Evidence-Data-START## Transcript exon combination :: BC068387.1, CD758366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505385, SAMEA4476770 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCCAAGT TATTCCGGGC TGTTATTATG GGTCCACCAG GCTCGGGGAA GGGGACCATC 
TCGGAGAGGA TCGCGCACAA TTTCGGCCTT AAACATTTGT CCAGTGGAGA TTTTGTTCGA 
GAAAACATCT CCTCAAAAAC TGATGCCGGT GTTTTAGCCA AGACGTATAT TAATAAAGGA 
CTGCTGGTTC CAGACCATGT GATGACACGT CTTTTGTTGC CTCGACTGGA GGAGATGACC 
AAATACAGCT GGCTGTTAGA TGGTTTTCCT CGGACACTGG CACAGGCTGA AGCTCTGAAC 
AGTTCCTGTG ACCTGGATGT AGCCATTAAC CTCAACATCC CTCTGGAAAC ACTGAAAGAA 
CGACTGCGAC ATCGATGGAT TCATCCACCC AGTGGGAGAG TGTACAATAT GTGCTTCAAT 
CCTCCGCGCA TCCAGGGGCT GGATGACATC ACAGGGGAGG CACTGATTCA GCAGGAGGAT 
GACAGACCAG AAGCCCTGGT GGCCAGACTG AGACACTACA AAGATGTTGC CAAACCTGTC 
ATAGACTTCT ATAAGGCTAA AGGCATCCTG TACACATTCT CGGACACAGA GACAGATCGA 
ATCTGGCCCA ATATCAACAC ACTTCTCAGC ACAAAGATCC CAGCCATCCA GTCAGATGCT 
CGCTGTGCTT CAACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998464.1
CDS	85..765
Translation	MSKLFRAVIMGPPGSGKGTISERIAHNFGLKHLSSGDFVRENISSKTDAGVLAKTYINKGLLVPDHVMTRLLLPRLEEMTKYSWLLDGFPRTLAQAEALNSSCDLDVAINLNIPLETLKERLRHRWIHPPSGRVYNMCFNPPRIQGLDDITGEALIQQEDDRPEALVARLRHYKDVAKPVIDFYKAKGILYTFSDTETDRIWPNINTLLSTKIPAIQSDARCASTR

Target ORF information:

RefSeq Version	NM_213299.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate kinase 4 (ak4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213299.1

ORF Insert Sequence:

ATGTCCAAGT TATTCCGGGC TGTTATTATG GGTCCACCAG GCTCGGGGAA GGGGACCATC 
TCGGAGAGGA TCGCGCACAA TTTCGGCCTT AAACATTTGT CCAGTGGAGA TTTTGTTCGA 
GAAAACATCT CCTCAAAAAC TGATGCCGGT GTTTTAGCCA AGACGTATAT TAATAAAGGA 
CTGCTGGTTC CAGACCATGT GATGACACGT CTTTTGTTGC CTCGACTGGA GGAGATGACC 
AAATACAGCT GGCTGTTAGA TGGTTTTCCT CGGACACTGG CACAGGCTGA AGCTCTGAAC 
AGTTCCTGTG ACCTGGATGT AGCCATTAAC CTCAACATCC CTCTGGAAAC ACTGAAAGAA 
CGACTGCGAC ATCGATGGAT TCATCCACCC AGTGGGAGAG TGTACAATAT GTGCTTCAAT 
CCTCCGCGCA TCCAGGGGCT GGATGACATC ACAGGGGAGG CACTGATTCA GCAGGAGGAT 
GACAGACCAG AAGCCCTGGT GGCCAGACTG AGACACTACA AAGATGTTGC CAAACCTGTC 
ATAGACTTCT ATAAGGCTAA AGGCATCCTG TACACATTCT CGGACACAGA GACAGATCGA 
ATCTGGCCCA ATATCAACAC ACTTCTCAGC ACAAAGATCC CAGCCATCCA GTCAGATGCT 
CGCTGTGCTT CAACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ak4 ( XM_005165873.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165930.1 Danio rerio adenylate kinase 4 (ak4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa11914
Clone ID Related Accession (Same CDS sequence)	XM_005165873.3 , XM_005165871.3
Accession Version	XM_005165873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenylate kinase 4, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGT TATTCCGGGC TGTTATTATG GGTCCACCAG GTTCGGGGAA GGGGACCATC 
TCGGAGAGGA TCGCGCACAA TTTCGGCCTT AAACATTTGT CCAGTGGAGA TTTTGTTCGA 
GAAAACATCT CCTCAAAAAC TGATGCCGGT GTTTTAGCCA AGACGTATAT TAATAAAGGA 
CTGCTGGTTC CAGACCATGT GATGACACGT CTTTTGTTGC CTCGACTGGA GGAGATGACC 
AAATACAGCT GGCTGTTAGA TGGTTTTCCT CGGACACTGG CACAGGCTGA AGCTCTGAAC 
AGTTCCTGTG ACCTGGATGT AGCCATTAAC CTCAACATCC CTCTGGAAAC ACTGAAAGAA 
CGACTGCGAC ATCGATGGAT TCATCCACCC AGTGGGAGAG TGTACAATAT GTGCTTCAAT 
CCTCCGCGCA TCCAGGGGCT GGATGACATC ACAGGGGAGG CACTGATTCA GCAGGAGGAT 
GACAGACCAG AAGCCCTGGT GGCCAGACTG AGACACTACA AAGATGTTGC CAAACCTGTC 
ATAGACTTCT ATAAGGCTAA AGGCATCCTG TACACATTCT CGGACACAGA GACAGATCGA 
ATCTGGCCCA ATATCAACAC ACTTCTCAGC ACAAAGATCC CAGCCATCCA GTCAGATGCT 
CGCTGCGCTT CAACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165930.1
CDS	625..1305
Translation	MSKLFRAVIMGPPGSGKGTISERIAHNFGLKHLSSGDFVRENISSKTDAGVLAKTYINKGLLVPDHVMTRLLLPRLEEMTKYSWLLDGFPRTLAQAEALNSSCDLDVAINLNIPLETLKERLRHRWIHPPSGRVYNMCFNPPRIQGLDDITGEALIQQEDDRPEALVARLRHYKDVAKPVIDFYKAKGILYTFSDTETDRIWPNINTLLSTKIPAIQSDARCASTR

Target ORF information:

RefSeq Version	XM_005165873.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate kinase 4 (ak4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165873.3

ORF Insert Sequence:

ATGTCCAAGT TATTCCGGGC TGTTATTATG GGTCCACCAG GTTCGGGGAA GGGGACCATC 
TCGGAGAGGA TCGCGCACAA TTTCGGCCTT AAACATTTGT CCAGTGGAGA TTTTGTTCGA 
GAAAACATCT CCTCAAAAAC TGATGCCGGT GTTTTAGCCA AGACGTATAT TAATAAAGGA 
CTGCTGGTTC CAGACCATGT GATGACACGT CTTTTGTTGC CTCGACTGGA GGAGATGACC 
AAATACAGCT GGCTGTTAGA TGGTTTTCCT CGGACACTGG CACAGGCTGA AGCTCTGAAC 
AGTTCCTGTG ACCTGGATGT AGCCATTAAC CTCAACATCC CTCTGGAAAC ACTGAAAGAA 
CGACTGCGAC ATCGATGGAT TCATCCACCC AGTGGGAGAG TGTACAATAT GTGCTTCAAT 
CCTCCGCGCA TCCAGGGGCT GGATGACATC ACAGGGGAGG CACTGATTCA GCAGGAGGAT 
GACAGACCAG AAGCCCTGGT GGCCAGACTG AGACACTACA AAGATGTTGC CAAACCTGTC 
ATAGACTTCT ATAAGGCTAA AGGCATCCTG TACACATTCT CGGACACAGA GACAGATCGA 
ATCTGGCCCA ATATCAACAC ACTTCTCAGC ACAAAGATCC CAGCCATCCA GTCAGATGCT 
CGCTGCGCTT CAACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ak4 ( XM_005165871.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165928.1 Danio rerio adenylate kinase 4 (ak4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa11914
Clone ID Related Accession (Same CDS sequence)	XM_005165873.3 , XM_005165871.3
Accession Version	XM_005165871.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	adenylate kinase 4, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165871.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGT TATTCCGGGC TGTTATTATG GGTCCACCAG GTTCGGGGAA GGGGACCATC 
TCGGAGAGGA TCGCGCACAA TTTCGGCCTT AAACATTTGT CCAGTGGAGA TTTTGTTCGA 
GAAAACATCT CCTCAAAAAC TGATGCCGGT GTTTTAGCCA AGACGTATAT TAATAAAGGA 
CTGCTGGTTC CAGACCATGT GATGACACGT CTTTTGTTGC CTCGACTGGA GGAGATGACC 
AAATACAGCT GGCTGTTAGA TGGTTTTCCT CGGACACTGG CACAGGCTGA AGCTCTGAAC 
AGTTCCTGTG ACCTGGATGT AGCCATTAAC CTCAACATCC CTCTGGAAAC ACTGAAAGAA 
CGACTGCGAC ATCGATGGAT TCATCCACCC AGTGGGAGAG TGTACAATAT GTGCTTCAAT 
CCTCCGCGCA TCCAGGGGCT GGATGACATC ACAGGGGAGG CACTGATTCA GCAGGAGGAT 
GACAGACCAG AAGCCCTGGT GGCCAGACTG AGACACTACA AAGATGTTGC CAAACCTGTC 
ATAGACTTCT ATAAGGCTAA AGGCATCCTG TACACATTCT CGGACACAGA GACAGATCGA 
ATCTGGCCCA ATATCAACAC ACTTCTCAGC ACAAAGATCC CAGCCATCCA GTCAGATGCT 
CGCTGCGCTT CAACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165928.1
CDS	629..1309
Translation	MSKLFRAVIMGPPGSGKGTISERIAHNFGLKHLSSGDFVRENISSKTDAGVLAKTYINKGLLVPDHVMTRLLLPRLEEMTKYSWLLDGFPRTLAQAEALNSSCDLDVAINLNIPLETLKERLRHRWIHPPSGRVYNMCFNPPRIQGLDDITGEALIQQEDDRPEALVARLRHYKDVAKPVIDFYKAKGILYTFSDTETDRIWPNINTLLSTKIPAIQSDARCASTR

Target ORF information:

RefSeq Version	XM_005165871.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio adenylate kinase 4 (ak4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165871.3

ORF Insert Sequence:

ATGTCCAAGT TATTCCGGGC TGTTATTATG GGTCCACCAG GTTCGGGGAA GGGGACCATC 
TCGGAGAGGA TCGCGCACAA TTTCGGCCTT AAACATTTGT CCAGTGGAGA TTTTGTTCGA 
GAAAACATCT CCTCAAAAAC TGATGCCGGT GTTTTAGCCA AGACGTATAT TAATAAAGGA 
CTGCTGGTTC CAGACCATGT GATGACACGT CTTTTGTTGC CTCGACTGGA GGAGATGACC 
AAATACAGCT GGCTGTTAGA TGGTTTTCCT CGGACACTGG CACAGGCTGA AGCTCTGAAC 
AGTTCCTGTG ACCTGGATGT AGCCATTAAC CTCAACATCC CTCTGGAAAC ACTGAAAGAA 
CGACTGCGAC ATCGATGGAT TCATCCACCC AGTGGGAGAG TGTACAATAT GTGCTTCAAT 
CCTCCGCGCA TCCAGGGGCT GGATGACATC ACAGGGGAGG CACTGATTCA GCAGGAGGAT 
GACAGACCAG AAGCCCTGGT GGCCAGACTG AGACACTACA AAGATGTTGC CAAACCTGTC 
ATAGACTTCT ATAAGGCTAA AGGCATCCTG TACACATTCT CGGACACAGA GACAGATCGA 
ATCTGGCCCA ATATCAACAC ACTTCTCAGC ACAAAGATCC CAGCCATCCA GTCAGATGCT 
CGCTGCGCTT CAACCCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Xmrk, kras and myc transgenic zebrafish liver cancer models share molecular signatures with subsets of human hepatocellular carcinoma.
PloS one9(3)e91179(2014)
Zheng W,Li Z,Nguyen AT,Li C,Emelyanov A,Gong Z

Drug screening in Scn1a zebrafish mutant identifies clemizole as a potential Dravet syndrome treatment.
Nature communications42410(2013)
Baraban SC,Dinday MT,Hortopan GA

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ak4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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