vgll4b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	vgll4b
Entrez Gene ID	406560
Full Name	vestigial-like family member 4b
Synonyms	vgll4,zgc:85699
General protein information	Preferred Names vestigial-like family member 4b Names transcription cofactor vestigial-like protein 4 vestigial like 4 vestigial like 4b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213275.2	NP_998440.1	transcription cofactor vestigial-like protein 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VGLL4	NP_001092598.1
Xenopus tropicalis (tropical clawed frog)	vgll4	NP_001072615.1
Mus musculus (house mouse)	Vgll4	NP_808351.1
Rattus norvegicus (Norway rat)	Vgll4	NP_001015004.1
	VGLL4	XP_002802743.1
Canis lupus familiaris (dog)	VGLL4	XP_005632204.1
Gallus gallus (chicken)	VGLL4	NP_001025764.1
Danio rerio (zebrafish)	vgll4b	NP_998440.1
Homo sapiens (human)	VGLL4	NP_001121691.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential expression of synapsin genes during early zebrafish development.
Garbarino G, Costa S, Pestarino M, Candiani S
Neuroscience280351-67(2014 Nov)

Establishment of transgenic lines to monitor and manipulate Yap/Taz-Tead activity in zebrafish reveals both evolutionarily conserved and divergent functions of the Hippo pathway.
Miesfeld JB, Link BA
Mechanisms of development133177-88(2014 Aug)

A morpholino-based screen to identify novel genes involved in craniofacial morphogenesis.
Melvin VS, Feng W, Hernandez-Lagunas L, Artinger KB, Williams T
Developmental dynamics : an official publication of the American Association of Anatomists242(7)817-31(2013 Jul)

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Wang Y, Zhang S
Functional & integrative genomics11(1)111-8(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vgll4b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vgll4b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02745	NM_213275.2 Latest version!	Danio rerio vestigial-like family member 4b (vgll4b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vgll4b ( NM_213275.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998440.1 Danio rerio vestigial-like family member 4b (vgll4b), mRNA.

Price & Availability↑

CloneID	ODa02745
Clone ID Related Accession (Same CDS sequence)	NM_213275.2
Accession Version	NM_213275.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-04
Organism	Danio rerio(zebrafish)
Product	transcription cofactor vestigial-like protein 4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068363.1. On Jun 10, 2016 this sequence version replaced NM_213275.1. ##Evidence-Data-START## Transcript exon combination :: BC068363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCTTTTTA CCAAAATGGA CCTGTTGAAC TACCAGTACT TGGACAAAAT GAACAACAAC 
ATCGGCATCC TGTGCTACGA AGGAGATGCG GCTCTGAGAG GCGAGTCCAG AATGCAGTCT 
CTGTCGTCTG CAGTCAGCAA CCACCGGACC GGCCCTCCTC CCATCAGCCC CAGCAAGAGG 
AAACACAGCG CGGAGCAAGC AGACGACGAC ATCGACTGCA ACAGTGAGCA TGTGGCCAAG 
ATGAGCCGCC TGTTCGCCGC ACAGCTAGGA AAGCCTGCCA ACGGGGACTA CCGCAAGGAC 
CCCCGGGAGC GCAGCCGCAG CCCCATCGAG CGCATGGCGG CCCCCAGCAT GAGTCTGGTT 
GGTGGGCATC ATCTCTACAT GCCCAGCCTG GCCCTGGACC AGCCGCTTGC ACTGACCAAA 
AACATGGACT CCAGCCGCAG CATGGGCATC TCTCCTACTG CCAGCCCTGT GGAGCGCCAG 
CAGAACCGGC CCTCTGTGAT CACCTGTGCT CCAGCTAACA ACCGTAACTG TAACCTCAGC 
CACTGCACCG GCTCCCACAA CGGCTGCTCC CCCGGCCTGA ACGCCAGCTA CAGGAGAGCC 
TCCAACTCGA ACACCGCCTG TGACCCTGTG ATCGAGGAGC ATTTCCGCCG CAGTCTGGGC 
AAGAACTACA AGGAGCCCGA GCCCGTCACA AACTCCGTGT CCATCACCGG CTCGGTGGAC 
GACCACTTCG CCAAGGCTCT GGGGGAAACG TGGCTGCAGA TCAAGGCTAA AGGAGGCTCC 
AGCTCCCCGG ACGCCTCTCC AAACACACAC ATGGTGAACC ACAATCACTC CCCGTCCCTG 
GTGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998440.1
CDS	145..993
Translation	MLFTKMDLLNYQYLDKMNNNIGILCYEGDAALRGESRMQSLSSAVSNHRTGPPPISPSKRKHSAEQADDDIDCNSEHVAKMSRLFAAQLGKPANGDYRKDPRERSRSPIERMAAPSMSLVGGHHLYMPSLALDQPLALTKNMDSSRSMGISPTASPVERQQNRPSVITCAPANNRNCNLSHCTGSHNGCSPGLNASYRRASNSNTACDPVIEEHFRRSLGKNYKEPEPVTNSVSITGSVDDHFAKALGETWLQIKAKGGSSSPDASPNTHMVNHNHSPSLVS

Target ORF information:

RefSeq Version	NM_213275.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio vestigial-like family member 4b (vgll4b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213275.2

ORF Insert Sequence:

ATGCTTTTTA CCAAAATGGA CCTGTTGAAC TACCAGTACT TGGACAAAAT GAACAACAAC 
ATCGGCATCC TGTGCTACGA AGGAGATGCG GCTCTGAGAG GCGAGTCCAG AATGCAGTCT 
CTGTCGTCTG CAGTCAGCAA CCACCGGACC GGCCCTCCTC CCATCAGCCC CAGCAAGAGG 
AAACACAGCG CGGAGCAAGC AGACGACGAC ATCGACTGCA ACAGTGAGCA TGTGGCCAAG 
ATGAGCCGCC TGTTCGCCGC ACAGCTAGGA AAGCCTGCCA ACGGGGACTA CCGCAAGGAC 
CCCCGGGAGC GCAGCCGCAG CCCCATCGAG CGCATGGCGG CCCCCAGCAT GAGTCTGGTT 
GGTGGGCATC ATCTCTACAT GCCCAGCCTG GCCCTGGACC AGCCGCTTGC ACTGACCAAA 
AACATGGACT CCAGCCGCAG CATGGGCATC TCTCCTACTG CCAGCCCTGT GGAGCGCCAG 
CAGAACCGGC CCTCTGTGAT CACCTGTGCT CCAGCTAACA ACCGTAACTG TAACCTCAGC 
CACTGCACCG GCTCCCACAA CGGCTGCTCC CCCGGCCTGA ACGCCAGCTA CAGGAGAGCC 
TCCAACTCGA ACACCGCCTG TGACCCTGTG ATCGAGGAGC ATTTCCGCCG CAGTCTGGGC 
AAGAACTACA AGGAGCCCGA GCCCGTCACA AACTCCGTGT CCATCACCGG CTCGGTGGAC 
GACCACTTCG CCAAGGCTCT GGGGGAAACG TGGCTGCAGA TCAAGGCTAA AGGAGGCTCC 
AGCTCCCCGG ACGCCTCTCC AAACACACAC ATGGTGAACC ACAATCACTC CCCGTCCCTG 
GTGTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential expression of synapsin genes during early zebrafish development.
Neuroscience280351-67(2014 Nov)
Garbarino G,Costa S,Pestarino M,Candiani S

Establishment of transgenic lines to monitor and manipulate Yap/Taz-Tead activity in zebrafish reveals both evolutionarily conserved and divergent functions of the Hippo pathway.
Mechanisms of development133177-88(2014 Aug)
Miesfeld JB,Link BA

A morpholino-based screen to identify novel genes involved in craniofacial morphogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists242(7)817-31(2013 Jul)
Melvin VS,Feng W,Hernandez-Lagunas L,Artinger KB,Williams T

Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and "pre-hepatic" acute phase response.
Functional & integrative genomics11(1)111-8(2011 Mar)
Wang Y,Zhang S