amfr cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	amfr
Entrez Gene ID	406442
Full Name	autocrine motility factor receptor a
Synonyms	amfr,gp78,wu:fi06e06,wu:fi37e05,zgc:63893
General protein information	Preferred Names autocrine motility factor receptor a Names E3 ubiquitin-protein ligase AMFR autocrine motility factor receptor, E3 ubiquitin protein ligase a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298211.2	XP_009296486.1	E3 ubiquitin-protein ligase AMFR isoform X1
NM_213163.2	NP_998328.2	E3 ubiquitin-protein ligase AMFR
NM_213163.1	NP_998328.1	E3 ubiquitin-protein ligase AMFR
XM_009298211.3	XP_009296486.1	E3 ubiquitin-protein ligase AMFR isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	AMFR	XP_005617971.1
Rattus norvegicus (Norway rat)	Amfr	XP_006255201.1
Pan troglodytes (chimpanzee)	AMFR	XP_001135064.2
Danio rerio (zebrafish)	amfr	NP_998328.1
Xenopus tropicalis (tropical clawed frog)	amfr	NP_001017083.1
Gallus gallus (chicken)	AMFR	XP_414064.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007538	XP_001237062.2
Bos taurus (cattle)	AMFR	NP_001039439.1
Mus musculus (house mouse)	Amfr	NP_035917.2
Homo sapiens (human)	AMFR	NP_001135.3
Caenorhabditis elegans (roundworm)	hrdl-1	NP_492602.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The E3 ubiquitin ligase gp78 protects against ER stress in zebrafish liver.
Chen Z, Ballar P, Fu Y, Luo J, Du S, Fang S
Journal of genetics and genomics = Yi chuan xue bao41(7)357-68(2014 Jul)

The Fanconi anemia gene network is conserved from zebrafish to human.
Titus TA, Selvig DR, Qin B, Wilson C, Starks AM, Roe BA, Postlethwait JH
Gene371(2)211-23(2006 Apr)

Many genes in fish have species-specific asymmetric rates of molecular evolution.
Steinke D, Salzburger W, Braasch I, Meyer A
BMC genomics720(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gp78 plays a critical role in protecting against ER stress in liver
Title: The E3 ubiquitin ligase gp78 protects against ER stress in zebrafish liver.

The following amfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the amfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00299	NM_213163.1 Latest version!	Danio rerio autocrine motility factor receptor a (amfra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa29688	NM_213163.2 Latest version!	Danio rerio autocrine motility factor receptor a (amfra), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa29688	XM_009298211.3 Latest version!	Danio rerio autocrine motility factor receptor, E3 ubiquitin protein ligase a (amfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa29688	XM_009298211.2	Danio rerio autocrine motility factor receptor, E3 ubiquitin protein ligase a (amfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

amfr ( NM_213163.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998328.1 Danio rerio autocrine motility factor receptor a (amfra), mRNA.

Price & Availability↑

CloneID	ODa00299
Clone ID Related Accession (Same CDS sequence)	NM_213163.1
Accession Version	NM_213163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-12
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase AMFR
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC057411.1. ##Evidence-Data-START## Transcript exon combination :: BC057411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCCGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCTCAC CCATACAGAT GAACGCGGCT TCTGAAGACG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998328.1
CDS	138..2000
Translation	MPLLFLERFPWPSLQTYTALSALLLAGTVLSAYSTVRDSEFSSALSEGPQNEELKLENLGNVATGVLLHLITDSLFVWVMVNTACCILMLIGKVIQCIVFGPLRVSEKQHLKDKFWNFIFYKFIFIFGVLNVQRVEEVVLWCLWFSMLIFLHLMVQLCKDRFEYLSFSPTTPMSSHVRVLALLVSVLSCCGGLAVLCALAGHIHGMHTVAFMAAECLLVTVRTGHVIIRYSIHLWDLNHEGTWENKSSYIYYTDFIMELAILSLDLMHHIHMLLFGNIWLSMASLVIFMQLRHLFHEVQRRIRRHKNYLRVIDNMESRFAVATPEELAANNDDCAICWDSMTTARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIHEGRREREEGQREPLEDNMAAAGAADARAHINQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIAQANNSQLNGMAHQIQEMFPQVPYHLILQDLQLTRSVEVTTDNILEGRIQVPFPTQSTDRSPIQMNAASEDGAGASSGSEVAAPEAEDFEVRGSRFSKSADERQRMLMQRKEELLLRARRRYLHKKPEDGDAYSVLGADNDDSVPSIEDEDSDSVTLRRRRLAAAAERRILRQEPSPF

Target ORF information:

RefSeq Version	NM_213163.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio autocrine motility factor receptor a (amfra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213163.1

ORF Insert Sequence:

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCCGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCTCAC CCATACAGAT GAACGCGGCT TCTGAAGACG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

amfr ( NM_213163.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998328.2 Danio rerio autocrine motility factor receptor a (amfra), mRNA.

Price & Availability↑

CloneID	ODa29688
Clone ID Related Accession (Same CDS sequence)	XM_009298211.2 , NM_213163.2 , XM_009298211.3
Accession Version	NM_213163.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase AMFR
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU855766.5. On or before Apr 17, 2018 this sequence version replaced XM_009298211.3, NM_213163.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC057411.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCAGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCACAC CCATACAGAT GAACGCGGCT TCTGAAGATG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998328.2
CDS	150..2012
Translation	MPLLFLERFPWPSLQTYTALSALLLAGTVLSAYSTVRDSEFSSALSEGPQNEELKLENLGNVATGVLLHLITDSLFVWVMVNTACCILMLIGKVIQCIVFGPLRVSEKQHLKDKFWNFIFYKFIFIFGVLNVQRVEEVVLWCLWFSMLIFLHLMVQLCKDRFEYLSFSPTTPMSSHVRVLALLVSVLSCCGGLAVLCALAGHIHGMHTVAFMAAECLLVTVRTGHVIIRYSIHLWDLNHEGTWENKSSYIYYTDFIMELAILSLDLMHHIHMLLFGNIWLSMASLVIFMQLRHLFHEVQRRIRRHKNYLRVIDNMESRFAVATPEELAANNDDCAICWDSMTTARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIHEGRREREEGQREPLEDNMAAAGAADARAHINQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIAQANNSQLNGMAHQIQEMFPQVPYHLILQDLQLTRSVEVTTDNILEGRIQVPFPTQSTDRTPIQMNAASEDGAGASSGSEVAAPEAEDFEVRGSRFSKSADERQRMLMQRKEELLLRARRRYLHKKPEDGDAYSVLGADNDDSVPSIEDEDSDSVTLRRRRLAAAAERRILRQEPSPF

Target ORF information:

RefSeq Version	NM_213163.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio autocrine motility factor receptor a (amfra), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213163.2

ORF Insert Sequence:

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCAGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCACAC CCATACAGAT GAACGCGGCT TCTGAAGATG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

amfr ( XM_009298211.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296486.1 Danio rerio autocrine motility factor receptor, E3 ubiquitin protein ligase a (amfr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa29688
Clone ID Related Accession (Same CDS sequence)	XM_009298211.2 , NM_213163.2 , XM_009298211.3
Accession Version	XM_009298211.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase AMFR isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298211.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCAGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCACAC CCATACAGAT GAACGCGGCT TCTGAAGATG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296486.1
CDS	166..2028
Translation	MPLLFLERFPWPSLQTYTALSALLLAGTVLSAYSTVRDSEFSSALSEGPQNEELKLENLGNVATGVLLHLITDSLFVWVMVNTACCILMLIGKVIQCIVFGPLRVSEKQHLKDKFWNFIFYKFIFIFGVLNVQRVEEVVLWCLWFSMLIFLHLMVQLCKDRFEYLSFSPTTPMSSHVRVLALLVSVLSCCGGLAVLCALAGHIHGMHTVAFMAAECLLVTVRTGHVIIRYSIHLWDLNHEGTWENKSSYIYYTDFIMELAILSLDLMHHIHMLLFGNIWLSMASLVIFMQLRHLFHEVQRRIRRHKNYLRVIDNMESRFAVATPEELAANNDDCAICWDSMTTARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIHEGRREREEGQREPLEDNMAAAGAADARAHINQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIAQANNSQLNGMAHQIQEMFPQVPYHLILQDLQLTRSVEVTTDNILEGRIQVPFPTQSTDRTPIQMNAASEDGAGASSGSEVAAPEAEDFEVRGSRFSKSADERQRMLMQRKEELLLRARRRYLHKKPEDGDAYSVLGADNDDSVPSIEDEDSDSVTLRRRRLAAAAERRILRQEPSPF

Target ORF information:

RefSeq Version	XM_009298211.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio autocrine motility factor receptor, E3 ubiquitin protein ligase a (amfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298211.2

ORF Insert Sequence:

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCAGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCACAC CCATACAGAT GAACGCGGCT TCTGAAGATG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

amfr ( XM_009298211.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296486.1 Danio rerio autocrine motility factor receptor, E3 ubiquitin protein ligase a (amfr), transcript variant X1, mRNA.

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CloneID	ODa29688
Clone ID Related Accession (Same CDS sequence)	XM_009298211.2 , NM_213163.2 , XM_009298211.3
Accession Version	XM_009298211.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase AMFR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298211.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCAGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCACAC CCATACAGAT GAACGCGGCT TCTGAAGATG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296486.1
CDS	175..2037
Translation	MPLLFLERFPWPSLQTYTALSALLLAGTVLSAYSTVRDSEFSSALSEGPQNEELKLENLGNVATGVLLHLITDSLFVWVMVNTACCILMLIGKVIQCIVFGPLRVSEKQHLKDKFWNFIFYKFIFIFGVLNVQRVEEVVLWCLWFSMLIFLHLMVQLCKDRFEYLSFSPTTPMSSHVRVLALLVSVLSCCGGLAVLCALAGHIHGMHTVAFMAAECLLVTVRTGHVIIRYSIHLWDLNHEGTWENKSSYIYYTDFIMELAILSLDLMHHIHMLLFGNIWLSMASLVIFMQLRHLFHEVQRRIRRHKNYLRVIDNMESRFAVATPEELAANNDDCAICWDSMTTARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIHEGRREREEGQREPLEDNMAAAGAADARAHINQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGIAQANNSQLNGMAHQIQEMFPQVPYHLILQDLQLTRSVEVTTDNILEGRIQVPFPTQSTDRTPIQMNAASEDGAGASSGSEVAAPEAEDFEVRGSRFSKSADERQRMLMQRKEELLLRARRRYLHKKPEDGDAYSVLGADNDDSVPSIEDEDSDSVTLRRRRLAAAAERRILRQEPSPF

Target ORF information:

RefSeq Version	XM_009298211.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio autocrine motility factor receptor, E3 ubiquitin protein ligase a (amfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298211.3

ORF Insert Sequence:

ATGCCTCTGC TGTTTCTGGA GCGTTTCCCC TGGCCTAGTT TGCAGACATA CACGGCCCTG 
TCAGCCCTCC TGCTGGCCGG GACCGTCCTG AGTGCCTATA GCACCGTCAG AGACTCAGAG 
TTCAGCTCTG CCCTCAGTGA AGGGCCCCAG AATGAGGAGC TGAAGCTGGA GAATCTGGGA 
AATGTGGCCA CCGGCGTCCT GCTGCACCTC ATCACTGACA GCCTGTTTGT TTGGGTGATG 
GTGAACACCG CCTGCTGCAT CCTGATGTTG ATAGGAAAAG TGATTCAGTG CATTGTGTTC 
GGGCCGCTGA GAGTCAGTGA GAAACAGCAT CTGAAGGATA AGTTCTGGAA CTTCATCTTC 
TACAAGTTCA TCTTCATCTT CGGTGTGCTG AACGTGCAGC GGGTGGAGGA GGTGGTGCTG 
TGGTGTCTGT GGTTCTCCAT GCTCATCTTC CTTCATCTGA TGGTGCAGCT CTGCAAAGAC 
CGCTTCGAAT ATCTCTCGTT TTCTCCCACG ACGCCGATGA GCAGTCATGT GCGAGTCCTG 
GCTCTGCTGG TGTCTGTACT GTCGTGTTGT GGTGGTTTAG CCGTGCTCTG CGCTCTCGCC 
GGTCATATTC ACGGCATGCA CACTGTCGCT TTCATGGCAG CCGAGTGTTT GCTAGTGACG 
GTGCGCACAG GACACGTCAT CATACGGTAT TCAATTCACC TGTGGGATCT GAACCATGAG 
GGAACCTGGG AGAACAAGAG CTCATATATT TACTACACAG ACTTCATCAT GGAGTTGGCA 
ATCCTCTCTC TGGACTTGAT GCATCACATA CATATGCTGC TCTTTGGAAA CATCTGGTTG 
TCCATGGCCA GCCTGGTGAT CTTCATGCAG CTGCGGCATC TCTTCCACGA GGTGCAGAGA 
CGAATCCGCA GACATAAAAA CTACCTGCGT GTCATCGACA ACATGGAGTC CAGATTTGCT 
GTGGCGACGC CGGAGGAGCT AGCCGCTAAT AACGATGACT GCGCCATCTG CTGGGACTCC 
ATGACCACAG CACGCAAACT ACCCTGCGGA CACCTTTTCC ACAATTCCTG TCTGCGCTCA 
TGGCTGGAGC AGGACACTTC CTGTCCCACC TGCCGAATGT CACTGAACAT CCATGAAGGC 
AGGCGGGAGC GGGAGGAGGG TCAGAGGGAG CCGCTGGAGG ACAATATGGC TGCTGCTGGA 
GCAGCAGATG CCCGGGCACA CATCAACCAG CACAACCACT TCTTCCACTT CGATGGTTCT 
CGTATCGCCA GCTGGCTGCC CAGTTTCTCA GTGGAGGTCA TGCACACCAC CAACATCCTG 
GGCATCGCTC AGGCCAACAA CTCTCAGCTC AACGGCATGG CCCATCAGAT CCAGGAGATG 
TTCCCGCAGG TGCCGTATCA TCTGATCCTG CAGGACCTTC AGCTCACACG CTCTGTGGAG 
GTCACCACCG ATAACATCCT GGAGGGCCGG ATTCAGGTGC CGTTCCCCAC ACAGTCCACA 
GATCGCACAC CCATACAGAT GAACGCGGCT TCTGAAGATG GAGCCGGAGC CAGCAGCGGT 
TCAGAGGTCG CGGCTCCAGA GGCTGAAGAC TTTGAGGTGC GCGGGAGCCG CTTTTCCAAA 
TCGGCAGATG AGAGACAGAG GATGCTAATG CAGAGGAAAG AGGAGCTGCT CCTAAGAGCT 
CGCAGACGTT ATCTGCACAA AAAACCTGAA GATGGAGATG CATATTCTGT TCTTGGCGCT 
GACAATGATG ACTCTGTGCC CTCCATTGAG GATGAAGACT CTGATTCAGT GACCCTGCGG 
CGCAGACGAC TGGCAGCCGC CGCTGAGAGA CGCATACTGA GGCAGGAGCC TTCCCCATTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The E3 ubiquitin ligase gp78 protects against ER stress in zebrafish liver.
Journal of genetics and genomics = Yi chuan xue bao41(7)357-68(2014 Jul)
Chen Z,Ballar P,Fu Y,Luo J,Du S,Fang S

The Fanconi anemia gene network is conserved from zebrafish to human.
Gene371(2)211-23(2006 Apr)
Titus TA,Selvig DR,Qin B,Wilson C,Starks AM,Roe BA,Postlethwait JH

Many genes in fish have species-specific asymmetric rates of molecular evolution.
BMC genomics720(2006 Feb)
Steinke D,Salzburger W,Braasch I,Meyer A

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amfr cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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