faf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	faf1
Entrez Gene ID	406243
Full Name	Fas (TNFRSF6) associated factor 1
Synonyms	wu:fc25h12,zgc:55752
General protein information	Preferred Names Fas (TNFRSF6) associated factor 1 Names FAS-associated factor 1 Fas associated factor 1 fc25h12
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212973.2	NP_998138.2	FAS-associated factor 1
XM_021478230.1	XP_021333905.1	FAS-associated factor 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FAF1	XP_001137725.1
Gallus gallus (chicken)	FAF1	XP_422464.4
Drosophila melanogaster (fruit fly)	casp	NP_611080.1
Homo sapiens (human)	FAF1	NP_008982.1
Danio rerio (zebrafish)	faf1	NP_998138.1
Canis lupus familiaris (dog)	FAF1	XP_849822.1
Bos taurus (cattle)	FAF1	NP_001039783.1
	FAF1	XP_001104146.2
Rattus norvegicus (Norway rat)	Faf1	NP_569090.1
Mus musculus (house mouse)	Faf1	NP_032009.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006473	XP_316513.4
Xenopus tropicalis (tropical clawed frog)	faf1	XP_002931465.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Ma Z, Zhu P, Pang M, Guo L, Chang N, Zheng J, Zhu X, Gao C, Huang H, Cui Z, Xiong JW, Peng J, Chen J
Scientific reports7(1)10381(2017 Sep)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish dmrta2 regulates the expression of cdkn2c in spermatogenesis in the adult testis.
Xu S, Xia W, Zohar Y, Gui JF
Biology of reproduction88(1)14(2013 Jan)

FAF1, a gene that is disrupted in cleft palate and has conserved function in zebrafish.
Ghassibe-Sabbagh M, Desmyter L, Langenberg T, Claes F, Boute O, Bayet B, Pellerin P, Hermans K, Backx L, Mansilla MA, Imoehl S, Nowak S, Ludwig KU, Baluardo C, Ferrian M, Mossey PA, Noethen M, Dewerchin M, Fran?ois G, Revencu N, Vanwijck R, Hecht J, Mangold E, Murray J, Rubini M, Vermeesch JR, Poirel HA, Carmeliet P, Vikkula M
American journal of human genetics88(2)150-61(2011 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following faf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the faf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa40838	NM_212973.2 Latest version!	Danio rerio Fas (TNFRSF6) associated factor 1 (faf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa143679	XM_021478230.1 Latest version!	Danio rerio Fas (TNFRSF6) associated factor 1 (faf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

faf1 ( NM_212973.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998138.2 Danio rerio Fas (TNFRSF6) associated factor 1 (faf1), mRNA.

Price & Availability↑

CloneID	ODa40838
Clone ID Related Accession (Same CDS sequence)	NM_212973.2
Accession Version	NM_212973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	FAS-associated factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR388216.11, DR723806.1, CN512998.1 and CU467870.6. On Aug 21, 2015 this sequence version replaced NM_212973.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01010073.1, BC048887.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTCCG ACAGCAACAT GGACAGAGAA ATGATCCTGG CCGACTTTCA GGCATGTACC 
GGAATTGACA ACATTGCTGA GGCGATCACA CTTCTAGAAC TAAACAACTG GGACTTGGTG 
GCGGCCATCA ATGGAGTGAT ACCACAAGAA AACGGGATCT TACAAAGTAG TTTCTCCAGT 
GAAGCATCTC AAACCGGCCC CTATGGACCT CACAGCCAAT CAGAAGCCAC AGCGCCTACG 
CCCTCAGCTC CACCTCCTTC TGCATCAGAA TCAGCATCAG CATCAGCATC ATCATCTTCC 
TCACCTCCGT CCTCCTCCAG CCCGGCCTTC TGCCCAGTCC AGCCCTCACG GCACATCGTT 
GAGCGGCAGT CCCGAATGCT CAGCTTCCGT GTAGAGTACA GGCAGCGCTC CATAGAGGTG 
GTGCTGGAGG ACGCCAGCAC AGTCGGTGAA ATCAAAACCA TTTTAGAGAC TGAGCTACAG 
GTGCCTGTCT CTAAAATGCA GTTAAAAGGC TGGAAGAGTG GAGACGTGTC TGACAGTACG 
GTTCTGCGGA GTTTACATTT GCCCAAGAAC AACAGCTTGT ATGTGTTAAC CCCAGATATC 
GCCCCTTCAG CCAGCACCAG CCAGAGCAGT GCCCAGCAAG GGTTGTCAAA TCAGAACTAC 
CTGCTCGTCA TTAGCCACCG TGAAGCACAG AGAGATTACA GCCTCAACTT CCCTGCCAGC 
AAAACCATCC AGGAGGTGAA AAGGAACATT TCAGACCTGA CAAACATCCC TGTGCGGCAT 
CAGCAGTGGG ACGGCTGGCC TGCTTCTGCC ACAGATGACT CGATGACTCT GGCCATCAGT 
GGAATCAGTT ACCCCTGTCA TCATCTGACC GTGTGCAGAA GATCTTCTCC TGCGCTCACA 
GTGGATCAGA CAGATGAGTG CACAGATGTC CACATGGTCA GTGACAGCGA GGGCGATGAT 
TTCGAAGATG CACCTGAATT TGGCGTAGAC GAATCAGAGA TCTTTGGCAT GGGGAGCTCG 
AGTTGTCGAA AGTCTCCAAT GATGCCAGAG AACAGTGAAA ATGAGGGTGA TGCCTTACTG 
CACTTTACTG CTGAATTTTC TAGAAGATAT GGCGATTGCC ATCCTGTGTT CTACATCGGC 
TCCTTGGAAG CAGCATCTCA AGAGGCCTTT TACGGCAAAG CCAGAGATAG GAAACTGCTG 
GCCATCTACC TCCACAACGA CGAGAGTGTC CTCAGTAATG TGTTCTGCTC TCAGATGATG 
TGTGCGGACT CCATAGTGTC ATATCTCAGC CAGAACTTCA TCACATGGGC CTGGGATGTT 
ACTAAAGAAG CAAATAAAGC CAGACTACTC ACGATGTGTA CAAGGCACTT TGGCAGCGTT 
GTTGCACAGA CCATTCGGAC ATACAAGACT GACCAGTTCC CATTACTTTT AATAGTCATG 
GGCAAACGGA CATCAAATGA AGTTTTAAAT GTCATTCAAG GCAACACAAC AGTGGACGAG 
CTTATGATGA GACTTATGGG AGCCATGGAG ATATTCACTG CACAGCAACA AGAAGACATC 
AAAGATGAGG ATGAACGTGA AGCCAGAGAA ATGGTCAAGA GAGAACAGGA TGAAGCTTAT 
CGTGTGTCTC TAGAAGCTGA TCGTAAAAAG AGAGAAGCGC AAGAGCGAGA AGAGGCGGAG 
CAAGTACGTC AGGAGCGAAT CAGGAAGGAG CAGGAGGAGG AACGAGAGGC TATCCGTCTC 
TCATTGGAGC AGGCCCTTCC ACCAGAGCCC AAAGAGGAGG GTGGAGAGCC AATCAGCAAA 
CTGCGCATCC GGACGCCAAG CGGTGAGTTT TTGGAAAGAC GGTTCCTGGG CACCTGTAAA 
CTCCAAGTCT TATTCGACTT CGTCGCCTCC AAGGGCTACC CATCCGACGA GTTTAAACTG 
CTCACCACCT TCCCACGCAG AAATATAACA CAGCTAGACC CGGCGTGGAA TCTGGTGGAG 
GCTAAACTCT TCCCTCAGGA GACCCTGTTT CTGGAGGCCA AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998138.2
CDS	266..2293
Translation	MASDSNMDREMILADFQACTGIDNIAEAITLLELNNWDLVAAINGVIPQENGILQSSFSSEASQTGPYGPHSQSEATAPTPSAPPPSASESASASASSSSSPPSSSSPAFCPVQPSRHIVERQSRMLSFRVEYRQRSIEVVLEDASTVGEIKTILETELQVPVSKMQLKGWKSGDVSDSTVLRSLHLPKNNSLYVLTPDIAPSASTSQSSAQQGLSNQNYLLVISHREAQRDYSLNFPASKTIQEVKRNISDLTNIPVRHQQWDGWPASATDDSMTLAISGISYPCHHLTVCRRSSPALTVDQTDECTDVHMVSDSEGDDFEDAPEFGVDESEIFGMGSSSCRKSPMMPENSENEGDALLHFTAEFSRRYGDCHPVFYIGSLEAASQEAFYGKARDRKLLAIYLHNDESVLSNVFCSQMMCADSIVSYLSQNFITWAWDVTKEANKARLLTMCTRHFGSVVAQTIRTYKTDQFPLLLIVMGKRTSNEVLNVIQGNTTVDELMMRLMGAMEIFTAQQQEDIKDEDEREAREMVKREQDEAYRVSLEADRKKREAQEREEAEQVRQERIRKEQEEEREAIRLSLEQALPPEPKEEGGEPISKLRIRTPSGEFLERRFLGTCKLQVLFDFVASKGYPSDEFKLLTTFPRRNITQLDPAWNLVEAKLFPQETLFLEAKE

Target ORF information:

RefSeq Version	NM_212973.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Fas (TNFRSF6) associated factor 1 (faf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212973.2

ORF Insert Sequence:

ATGGCCTCCG ACAGCAACAT GGACAGAGAA ATGATCCTGG CCGACTTTCA GGCATGTACC 
GGAATTGACA ACATTGCTGA GGCGATCACA CTTCTAGAAC TAAACAACTG GGACTTGGTG 
GCGGCCATCA ATGGAGTGAT ACCACAAGAA AACGGGATCT TACAAAGTAG TTTCTCCAGT 
GAAGCATCTC AAACCGGCCC CTATGGACCT CACAGCCAAT CAGAAGCCAC AGCGCCTACG 
CCCTCAGCTC CACCTCCTTC TGCATCAGAA TCAGCATCAG CATCAGCATC ATCATCTTCC 
TCACCTCCGT CCTCCTCCAG CCCGGCCTTC TGCCCAGTCC AGCCCTCACG GCACATCGTT 
GAGCGGCAGT CCCGAATGCT CAGCTTCCGT GTAGAGTACA GGCAGCGCTC CATAGAGGTG 
GTGCTGGAGG ACGCCAGCAC AGTCGGTGAA ATCAAAACCA TTTTAGAGAC TGAGCTACAG 
GTGCCTGTCT CTAAAATGCA GTTAAAAGGC TGGAAGAGTG GAGACGTGTC TGACAGTACG 
GTTCTGCGGA GTTTACATTT GCCCAAGAAC AACAGCTTGT ATGTGTTAAC CCCAGATATC 
GCCCCTTCAG CCAGCACCAG CCAGAGCAGT GCCCAGCAAG GGTTGTCAAA TCAGAACTAC 
CTGCTCGTCA TTAGCCACCG TGAAGCACAG AGAGATTACA GCCTCAACTT CCCTGCCAGC 
AAAACCATCC AGGAGGTGAA AAGGAACATT TCAGACCTGA CAAACATCCC TGTGCGGCAT 
CAGCAGTGGG ACGGCTGGCC TGCTTCTGCC ACAGATGACT CGATGACTCT GGCCATCAGT 
GGAATCAGTT ACCCCTGTCA TCATCTGACC GTGTGCAGAA GATCTTCTCC TGCGCTCACA 
GTGGATCAGA CAGATGAGTG CACAGATGTC CACATGGTCA GTGACAGCGA GGGCGATGAT 
TTCGAAGATG CACCTGAATT TGGCGTAGAC GAATCAGAGA TCTTTGGCAT GGGGAGCTCG 
AGTTGTCGAA AGTCTCCAAT GATGCCAGAG AACAGTGAAA ATGAGGGTGA TGCCTTACTG 
CACTTTACTG CTGAATTTTC TAGAAGATAT GGCGATTGCC ATCCTGTGTT CTACATCGGC 
TCCTTGGAAG CAGCATCTCA AGAGGCCTTT TACGGCAAAG CCAGAGATAG GAAACTGCTG 
GCCATCTACC TCCACAACGA CGAGAGTGTC CTCAGTAATG TGTTCTGCTC TCAGATGATG 
TGTGCGGACT CCATAGTGTC ATATCTCAGC CAGAACTTCA TCACATGGGC CTGGGATGTT 
ACTAAAGAAG CAAATAAAGC CAGACTACTC ACGATGTGTA CAAGGCACTT TGGCAGCGTT 
GTTGCACAGA CCATTCGGAC ATACAAGACT GACCAGTTCC CATTACTTTT AATAGTCATG 
GGCAAACGGA CATCAAATGA AGTTTTAAAT GTCATTCAAG GCAACACAAC AGTGGACGAG 
CTTATGATGA GACTTATGGG AGCCATGGAG ATATTCACTG CACAGCAACA AGAAGACATC 
AAAGATGAGG ATGAACGTGA AGCCAGAGAA ATGGTCAAGA GAGAACAGGA TGAAGCTTAT 
CGTGTGTCTC TAGAAGCTGA TCGTAAAAAG AGAGAAGCGC AAGAGCGAGA AGAGGCGGAG 
CAAGTACGTC AGGAGCGAAT CAGGAAGGAG CAGGAGGAGG AACGAGAGGC TATCCGTCTC 
TCATTGGAGC AGGCCCTTCC ACCAGAGCCC AAAGAGGAGG GTGGAGAGCC AATCAGCAAA 
CTGCGCATCC GGACGCCAAG CGGTGAGTTT TTGGAAAGAC GGTTCCTGGG CACCTGTAAA 
CTCCAAGTCT TATTCGACTT CGTCGCCTCC AAGGGCTACC CATCCGACGA GTTTAAACTG 
CTCACCACCT TCCCACGCAG AAATATAACA CAGCTAGACC CGGCGTGGAA TCTGGTGGAG 
GCTAAACTCT TCCCTCAGGA GACCCTGTTT CTGGAGGCCA AAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

faf1 ( XM_021478230.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333905.1 Danio rerio Fas (TNFRSF6) associated factor 1 (faf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa143679
Clone ID Related Accession (Same CDS sequence)	XM_021478230.1
Accession Version	XM_021478230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	FAS-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCCG ACAGCAACAT GGACAGAGAA ATGATCCTGG CCGACTTTCA GGCATGTACC 
GGAATTGACA ACATTGCTGA GGCGATCACA CTTCTAGAAC TAAACAACTG GGACTTGGTG 
GCGGCCATCA ATGGAGTGAT ACCACAAGAA AACGGGATCT TACAAAGTTT CTCCAGTGAA 
GCATCTCAAA CCGGCCCCTA TGGACCTCAC AGCCAATCAG AAGCCACAGC GCCTACGCCC 
TCAGCTCCAC CTCCTTCTGC ATCAGAATCA GCATCAGCAT CAGCATCATC ATCTTCCTCA 
CCTCCGTCCT CCTCCAGCCC GGCCTTCTGC CCAGTCCAGC CCTCACGGCA CATCGTTGAG 
CGGCAGTCCC GAATGCTCAG CTTCCGTGTA GAGTACAGGC AGCGCTCCAT AGAGGTGGTG 
CTGGAGGACG CCAGCACAGT CGGTGAAATC AAAACCATTT TAGAGACTGA GCTACAGGTG 
CCTGTCTCTA AAATGCAGTT AAAAGGCTGG AAGAGTGGAG ACGTGTCTGA CAGTACGGTT 
CTGCGGAGTT TACATTTGCC CAAGAACAAC AGCTTGTATG TGTTAACCCC AGATATCGCC 
CCTTCAGCCA GCACCAGCCA GAGCAGTGCC CAGCAAGGGT TGTCAAATCA GAACTACCTG 
CTCGTCATTA GCCACCGTGA AGCACAGAGA GATTACAGCC TCAACTTCCC TGCCAGCAAA 
ACCATCCAGG AGGTGAAAAG GAACATTTCA GACCTGACAA ACATCCCTGT GCGGCATCAG 
CAGTGGGACG GCTGGCCTGC TTCTGCCACA GATGACTCGA TGACTCTGGC CATCAGTGGA 
ATCAGTTACC CCTGTCATCA TCTGACCGTG TGCAGAAGAT CTTCTCCTGC GCTCACAGTG 
GATCAGACAG ATGAGTGCAC AGATGTCCAC ATGGTCAGTG ACAGCGAGGG CGATGATTTC 
GAAGATGCAC CTGAATTTGG CGTAGACGAA TCAGAGATCT TTGGCATGGG GAGCTCGAGT 
TGTCGAAAGT CTCCAATGAT GCCAGAGAAC AGTGAAAATG AGGGTGATGC CTTACTGCAC 
TTTACTGCTG AATTTTCTAG AAGATATGGC GATTGCCATC CTGTGTTCTA CATCGGCTCC 
TTGGAAGCAG CATCTCAAGA GGCCTTTTAC GGCAAAGCCA GAGATAGGAA ACTGCTGGCC 
ATCTACCTCC ACAACGACGA GAGTGTCCTC AGTAATGTGT TCTGCTCTCA GATGATGTGT 
GCGGACTCCA TAGTGTCATA TCTCAGCCAG AACTTCATCA CATGGGCCTG GGATGTTACT 
AAAGAAGCAA ATAAAGCCAG ACTACTCACG ATGTGTACAA GGCACTTTGG CAGCGTTGTT 
GCACAGACCA TTCGGACATA CAAGACTGAC CAGTTCCCAT TACTTTTAAT AGTCATGGGC 
AAACGGACAT CAAATGAAGT TTTAAATGTC ATTCAAGGCA ACACAACAGT GGACGAGCTT 
ATGATGAGAC TTATGGGAGC CATGGAGATA TTCACTGCAC AGCAACAAGA AGACATCAAA 
GATGAGGATG AACGTGAAGC CAGAGAAATG GTCAAGAGAG AACAGGATGA AGCTTATCGT 
GTGTCTCTAG AAGCTGATCG TAAAAAGAGA GAAGCGCAAG AGCGAGAAGA GGCGGAGCAA 
GTACGTCAGG AGCGAATCAG GAAGGAGCAG GAGGAGGAAC GAGAGGCTAT CCGTCTCTCA 
TTGGAGCAGG CCCTTCCACC AGAGCCCAAA GAGGAGGGTG GAGAGCCAAT CAGCAAACTG 
CGCATCCGGA CGCCAAGCGG TGAGTTTTTG GAAAGACGGT TCCTGGGCAC CTGTAAACTC 
CAAGTCTTAT TCGACTTCGT CGCCTCCAAG GGCTACCCAT CCGACGAGTT TAAACTGCTC 
ACCACCTTCC CACGCAGAAA TATAACACAG CTAGACCCGG CGTGGAATCT GGTGGAGGCT 
AAACTCTTCC CTCAGGAGAC CCTGTTTCTG GAGGCCAAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333905.1
CDS	232..2256
Translation	MASDSNMDREMILADFQACTGIDNIAEAITLLELNNWDLVAAINGVIPQENGILQSFSSEASQTGPYGPHSQSEATAPTPSAPPPSASESASASASSSSSPPSSSSPAFCPVQPSRHIVERQSRMLSFRVEYRQRSIEVVLEDASTVGEIKTILETELQVPVSKMQLKGWKSGDVSDSTVLRSLHLPKNNSLYVLTPDIAPSASTSQSSAQQGLSNQNYLLVISHREAQRDYSLNFPASKTIQEVKRNISDLTNIPVRHQQWDGWPASATDDSMTLAISGISYPCHHLTVCRRSSPALTVDQTDECTDVHMVSDSEGDDFEDAPEFGVDESEIFGMGSSSCRKSPMMPENSENEGDALLHFTAEFSRRYGDCHPVFYIGSLEAASQEAFYGKARDRKLLAIYLHNDESVLSNVFCSQMMCADSIVSYLSQNFITWAWDVTKEANKARLLTMCTRHFGSVVAQTIRTYKTDQFPLLLIVMGKRTSNEVLNVIQGNTTVDELMMRLMGAMEIFTAQQQEDIKDEDEREAREMVKREQDEAYRVSLEADRKKREAQEREEAEQVRQERIRKEQEEEREAIRLSLEQALPPEPKEEGGEPISKLRIRTPSGEFLERRFLGTCKLQVLFDFVASKGYPSDEFKLLTTFPRRNITQLDPAWNLVEAKLFPQETLFLEAKE

Target ORF information:

RefSeq Version	XM_021478230.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Fas (TNFRSF6) associated factor 1 (faf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478230.1

ORF Insert Sequence:

ATGGCCTCCG ACAGCAACAT GGACAGAGAA ATGATCCTGG CCGACTTTCA GGCATGTACC 
GGAATTGACA ACATTGCTGA GGCGATCACA CTTCTAGAAC TAAACAACTG GGACTTGGTG 
GCGGCCATCA ATGGAGTGAT ACCACAAGAA AACGGGATCT TACAAAGTTT CTCCAGTGAA 
GCATCTCAAA CCGGCCCCTA TGGACCTCAC AGCCAATCAG AAGCCACAGC GCCTACGCCC 
TCAGCTCCAC CTCCTTCTGC ATCAGAATCA GCATCAGCAT CAGCATCATC ATCTTCCTCA 
CCTCCGTCCT CCTCCAGCCC GGCCTTCTGC CCAGTCCAGC CCTCACGGCA CATCGTTGAG 
CGGCAGTCCC GAATGCTCAG CTTCCGTGTA GAGTACAGGC AGCGCTCCAT AGAGGTGGTG 
CTGGAGGACG CCAGCACAGT CGGTGAAATC AAAACCATTT TAGAGACTGA GCTACAGGTG 
CCTGTCTCTA AAATGCAGTT AAAAGGCTGG AAGAGTGGAG ACGTGTCTGA CAGTACGGTT 
CTGCGGAGTT TACATTTGCC CAAGAACAAC AGCTTGTATG TGTTAACCCC AGATATCGCC 
CCTTCAGCCA GCACCAGCCA GAGCAGTGCC CAGCAAGGGT TGTCAAATCA GAACTACCTG 
CTCGTCATTA GCCACCGTGA AGCACAGAGA GATTACAGCC TCAACTTCCC TGCCAGCAAA 
ACCATCCAGG AGGTGAAAAG GAACATTTCA GACCTGACAA ACATCCCTGT GCGGCATCAG 
CAGTGGGACG GCTGGCCTGC TTCTGCCACA GATGACTCGA TGACTCTGGC CATCAGTGGA 
ATCAGTTACC CCTGTCATCA TCTGACCGTG TGCAGAAGAT CTTCTCCTGC GCTCACAGTG 
GATCAGACAG ATGAGTGCAC AGATGTCCAC ATGGTCAGTG ACAGCGAGGG CGATGATTTC 
GAAGATGCAC CTGAATTTGG CGTAGACGAA TCAGAGATCT TTGGCATGGG GAGCTCGAGT 
TGTCGAAAGT CTCCAATGAT GCCAGAGAAC AGTGAAAATG AGGGTGATGC CTTACTGCAC 
TTTACTGCTG AATTTTCTAG AAGATATGGC GATTGCCATC CTGTGTTCTA CATCGGCTCC 
TTGGAAGCAG CATCTCAAGA GGCCTTTTAC GGCAAAGCCA GAGATAGGAA ACTGCTGGCC 
ATCTACCTCC ACAACGACGA GAGTGTCCTC AGTAATGTGT TCTGCTCTCA GATGATGTGT 
GCGGACTCCA TAGTGTCATA TCTCAGCCAG AACTTCATCA CATGGGCCTG GGATGTTACT 
AAAGAAGCAA ATAAAGCCAG ACTACTCACG ATGTGTACAA GGCACTTTGG CAGCGTTGTT 
GCACAGACCA TTCGGACATA CAAGACTGAC CAGTTCCCAT TACTTTTAAT AGTCATGGGC 
AAACGGACAT CAAATGAAGT TTTAAATGTC ATTCAAGGCA ACACAACAGT GGACGAGCTT 
ATGATGAGAC TTATGGGAGC CATGGAGATA TTCACTGCAC AGCAACAAGA AGACATCAAA 
GATGAGGATG AACGTGAAGC CAGAGAAATG GTCAAGAGAG AACAGGATGA AGCTTATCGT 
GTGTCTCTAG AAGCTGATCG TAAAAAGAGA GAAGCGCAAG AGCGAGAAGA GGCGGAGCAA 
GTACGTCAGG AGCGAATCAG GAAGGAGCAG GAGGAGGAAC GAGAGGCTAT CCGTCTCTCA 
TTGGAGCAGG CCCTTCCACC AGAGCCCAAA GAGGAGGGTG GAGAGCCAAT CAGCAAACTG 
CGCATCCGGA CGCCAAGCGG TGAGTTTTTG GAAAGACGGT TCCTGGGCAC CTGTAAACTC 
CAAGTCTTAT TCGACTTCGT CGCCTCCAAG GGCTACCCAT CCGACGAGTT TAAACTGCTC 
ACCACCTTCC CACGCAGAAA TATAACACAG CTAGACCCGG CGTGGAATCT GGTGGAGGCT 
AAACTCTTCC CTCAGGAGAC CCTGTTTCTG GAGGCCAAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants.
Scientific reports7(1)10381(2017 Sep)
Ma Z,Zhu P,Pang M,Guo L,Chang N,Zheng J,Zhu X,Gao C,Huang H,Cui Z,Xiong JW,Peng J,Chen J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish dmrta2 regulates the expression of cdkn2c in spermatogenesis in the adult testis.
Biology of reproduction88(1)14(2013 Jan)
Xu S,Xia W,Zohar Y,Gui JF

FAF1, a gene that is disrupted in cleft palate and has conserved function in zebrafish.
American journal of human genetics88(2)150-61(2011 Feb)
Ghassibe-Sabbagh M,Desmyter L,Langenberg T,Claes F,Boute O,Bayet B,Pellerin P,Hermans K,Backx L,Mansilla MA,Imoehl S,Nowak S,Ludwig KU,Baluardo C,Ferrian M,Mossey PA,Noethen M,Dewerchin M,Fran?ois G,Revencu N,Vanwijck R,Hecht J,Mangold E,Murray J,Rubini M,Vermeesch JR,Poirel HA,Carmeliet P,Vikkula M

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faf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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