SPAST cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAST
Entrez Gene ID	405851
Full Name	spastin
Synonyms	spg4,zgc:85952
General protein information	Preferred Names spastin Names spastin
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_212915.2	NP_998080.2	spastin
XM_005155397.3	XP_005155454.1	spastin isoform X1
XM_005155397.2	XP_005155454.1	spastin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPAST	NP_055761.2
Oryza sativa (rice)	Os06g0130000	NP_001056686.1
Xenopus tropicalis (tropical clawed frog)	spast	NP_001016453.1
	SPAST	XP_002799248.1
Canis lupus familiaris (dog)	SPAST	XP_850973.1
Drosophila melanogaster (fruit fly)	spas	NP_651206.3
Pan troglodytes (chimpanzee)	SPAST	XP_001164367.1
Mus musculus (house mouse)	Spast	NP_001156342.1
Rattus norvegicus (Norway rat)	Spast	NP_001102172.2
Danio rerio (zebrafish)	spast	NP_998080.1
Arabidopsis thaliana (thale cress)	AT2G45500	NP_182074.4
Bos taurus (cattle)	SPAST	NP_001075060.1
Gallus gallus (chicken)	SPAST	NP_001026232.1
Anopheles gambiae (African malaria mosquito)	SPAST_ANOGA	XP_312634.5
Caenorhabditis elegans (roundworm)	spas-1	NP_741586.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary recruitment of flexible Esrp-dependent splicing programs into diverse embryonic morphogenetic processes.
Burguera D, Marquez Y, Racioppi C, Permanyer J, Torres-M?ndez A, Esposito R, Albuixech-Crespo B, Fanlo L, D'Agostino Y, Gohr A, Navas-Perez E, Riesgo A, Cuomo C, Benvenuto G, Christiaen LA, Mart? E, D'Aniello S, Spagnuolo A, Ristoratore F, Arnone MI, Garcia-Fern?ndez J, Irimia M
Nature communications8(1)1799(2017 Nov)

Conserved pharmacological rescue of hereditary spastic paraplegia-related phenotypes across model organisms.
Julien C, Lissouba A, Madabattula S, Fardghassemi Y, Rosenfelt C, Androschuk A, Strautman J, Wong C, Bysice A, O'sullivan J, Rouleau GA, Drapeau P, Parker JA, Bolduc FV
Human molecular genetics25(6)1088-99(2016 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that the spinal cord motor neuron axon outgrowth of zebrafish is regulated by the interaction between spastin and protrudin.
Title: Role of spastin and protrudin in neurite outgrowth.

Simultaneous knockdown of spastin and katanin caused a more severe phenotype than did individual knockdown of either gene, suggesting that they have different but related functions in supporting axon outgrowth.
Title: Genetic and chemical modulation of spastin-dependent axon outgrowth in zebrafish embryos indicates a role for impaired microtubule dynamics in hereditary spastic paraplegia.

Reduced spg4 function caused dramatic defects in motor axon outgrowth without affecting the events driving the initial specification of motor neurones.
Title: The microtubule-severing protein Spastin is essential for axon outgrowth in the zebrafish embryo.

The following SPAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa06190	XM_005155397.3 Latest version!	Danio rerio spastin (spast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa98506	NM_212915.2 Latest version!	Danio rerio spastin (spast), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
ODa06190	XM_005155397.2	Danio rerio spastin (spast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAST ( XM_005155397.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155454.1 Danio rerio spastin (spast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06190
Clone ID Related Accession (Same CDS sequence)	XM_005155397.3 , XM_005155397.2
Accession Version	XM_005155397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
ACTGGCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155454.1
CDS	140..1771
Translation	MNSGHKARLRGGRACGPVSDGSARGNRLLFYTRSLSRVPEWLLRVLLLLLRWLFQPIRRAMAARAKECGPDGSEETGERIRNYHKQAFEFISVALQIDEDEKGDKQKAVQWYRKGIAELEKGIQIQVTGAGEKADRARKLQDKMITNLSMAEDRLKLLAPASGAVSKKKDTLTITNQTSLRPKNPPKSTPNASGLNCTPSAAQSSRTGPQNNQKGPTVKGKNNVKASTTATASPQRKRDMKNFKNVDSKLASLILNEIVDSGSVVRFDDIAGQDLAKQALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAMESNATFFNISAATLTSKYVGEGEKLVRALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSGGDERVLVMGATNRPQELDEAVLRRFAKRIYVALPTEETRLKLLKNLLSKHRNPLSQKELSQLARLTDGYSGSDLTSLAKDAALGPIRELKPEQVRNMSAHEMRDIRISDFLESLKRIKRSVSPQTLDQYVRWNREYGDTTGV

Target ORF information:

RefSeq Version	XM_005155397.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spastin (spast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155397.3

ORF Insert Sequence:

ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
ACTGGCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005155397.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005155454.1 Danio rerio spastin (spast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06190
Clone ID Related Accession (Same CDS sequence)	XM_005155397.3 , XM_005155397.2
Accession Version	XM_005155397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	spastin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005155397.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
ACTGGCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155454.1
CDS	121..1752
Translation	MNSGHKARLRGGRACGPVSDGSARGNRLLFYTRSLSRVPEWLLRVLLLLLRWLFQPIRRAMAARAKECGPDGSEETGERIRNYHKQAFEFISVALQIDEDEKGDKQKAVQWYRKGIAELEKGIQIQVTGAGEKADRARKLQDKMITNLSMAEDRLKLLAPASGAVSKKKDTLTITNQTSLRPKNPPKSTPNASGLNCTPSAAQSSRTGPQNNQKGPTVKGKNNVKASTTATASPQRKRDMKNFKNVDSKLASLILNEIVDSGSVVRFDDIAGQDLAKQALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAMESNATFFNISAATLTSKYVGEGEKLVRALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSGGDERVLVMGATNRPQELDEAVLRRFAKRIYVALPTEETRLKLLKNLLSKHRNPLSQKELSQLARLTDGYSGSDLTSLAKDAALGPIRELKPEQVRNMSAHEMRDIRISDFLESLKRIKRSVSPQTLDQYVRWNREYGDTTGV

Target ORF information:

RefSeq Version	XM_005155397.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio spastin (spast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155397.2

ORF Insert Sequence:

ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGCTCCA 
GCCAGTGGAG CAGTCTCCAA GAAGAAGGAC ACTCTCACAA TCACAAATCA AACTTCTCTG 
CGGCCCAAAA ATCCTCCCAA ATCTACCCCG AATGCATCAG GTCTGAACTG CACTCCCTCA 
GCAGCTCAGA GCAGCAGGAC CGGCCCTCAA AACAATCAGA AGGGGCCCAC AGTCAAAGGC 
AAAAATAATG TTAAAGCCAG TACCACTGCC ACTGCGTCAC CTCAAAGGAA AAGAGACATG 
AAGAACTTCA AAAATGTGGA CAGCAAACTG GCAAGCCTCA TCCTCAACGA GATCGTGGAC 
AGCGGGTCTG TGGTGAGGTT TGATGATATA GCAGGACAGG ATTTGGCCAA GCAGGCGCTG 
CAGGAAATCG TCATCCTGCC TGCCCTACGT CCAGAGTTGT TTACTGGTCT CCGGGCTCCA 
GCTAGAGGAC TCCTGCTCTT CGGCCCTCCT GGTAATGGGA AAACCATGCT GGCGAAAGCC 
GTCGCAATGG AATCGAATGC TACTTTCTTC AACATCAGCG CTGCCACTCT CACCTCCAAA 
TATGTGGGAG AGGGAGAGAA GCTGGTTCGA GCTCTGTTTG CGGTCGCTCG AGAGCTGCAG 
CCGTCGATTA TCTTCATCGA TGAGATCGAC AGTCTGCTGT GTGAGAGGAG GGAAGGCGAA 
CACGATGCCA GCAGACGACT CAAAACAGAG TTTCTCATCG AGTTTGATGG AGTGCAGTCA 
GGTGGAGATG AGCGTGTGTT AGTGATGGGA GCCACTAACA GGCCTCAGGA GCTGGACGAG 
GCTGTTCTCA GGAGATTTGC TAAACGCATT TATGTGGCTT TACCTACAGA GGAGACTCGA 
CTGAAGCTGC TGAAGAATCT CCTCAGTAAA CACAGAAATC CTCTGTCTCA GAAAGAGCTC 
AGTCAGCTGG CCAGGCTGAC CGACGGTTAC TCTGGCAGCG ATCTCACCTC TCTGGCTAAA 
GACGCTGCTC TCGGCCCGAT ACGAGAGCTG AAGCCGGAGC AGGTGAGGAA CATGTCAGCC 
CATGAGATGC GTGACATTCG CATCTCTGAT TTCCTGGAGT CTCTGAAGCG GATCAAGCGG 
AGCGTCAGTC CGCAGACCTT GGATCAGTAC GTGCGCTGGA ACCGCGAGTA CGGAGACACG 
ACTGGCGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( NM_212915.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_998080.2 Danio rerio spastin (spast), mRNA.

Price & Availability↑

CloneID	ODa98506
Clone ID Related Accession (Same CDS sequence)	NM_212915.2
Accession Version	NM_212915.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Danio rerio(zebrafish)
Product	spastin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY304504.1 and CU651563.7. On Oct 9, 2016 this sequence version replaced NM_212915.1. ##Evidence-Data-START## Transcript exon combination :: AY304504.1, BC067715.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGGCAAT 
CTGCTTTCAC AGTCTCCAGC AGAGAGCAGC TCTGATGATT CTTTCTACTC TTTCTCCAAT 
GGTAATCTGC GTCCAGCTCC AGCCAGTGGA GCAGTCTCCA AGAAGAAGGA CACTCTCACA 
ATCACAAATC AAACTTCTCT GCGGCCCAAA AATCCTCCCA AATCTACCCC GAATGCATCA 
GGTCTGAACT GCACTCCCTC AGCAGCTCAG AGCAGCAGGA CCGGCCCTCA AAACAATCAG 
AAGGGGCCCA CAGTCAAAGG CAAAAATAAT GTTAAAGCCA GTACCACTGC CACTGCGTCA 
CCTCAAAGGA AAAGAGACAT GAAGAACTTC AAAAATGTGG ACAGCAAACT GGCAAGCCTC 
ATCCTCAACG AGATCGTGGA CAGCGGGTCT GTGGTGAGGT TTGATGATAT AGCAGGACAG 
GATTTGGCCA AGCAGGCGCT GCAGGAAATC GTCATCCTGC CTGCCCTACG TCCAGAGTTG 
TTTACTGGTC TCCGGGCTCC AGCTAGAGGA CTCCTGCTCT TCGGCCCTCC TGGTAATGGG 
AAAACCATGC TGGCGAAAGC CGTCGCAATG GAATCGAATG CTACTTTCTT CAACATCAGC 
GCTGCCACTC TCACCTCCAA ATATGTGGGA GAGGGAGAGA AGCTGGTTCG AGCTCTGTTT 
GCGGTCGCTC GAGAGCTGCA GCCGTCGATT ATCTTCATCG ATGAGATCGA CAGTCTGCTG 
TGTGAGAGGA GGGAAGGCGA ACACGATGCC AGCAGACGAC TCAAAACAGA GTTTCTCATC 
GAGTTTGATG GAGTGCAGTC AGGTGGAGAT GAGCGTGTGT TAGTGATGGG AGCCACTAAC 
AGGCCTCAGG AGCTGGACGA GGCTGTTCTC AGGAGATTTG CTAAACGCAT TTATGTGGCT 
TTACCTACAG AGGAGACTCG ACTGAAGCTG CTGAAGAATC TCCTCAGTAA ACACAGAAAT 
CCTCTGTCTC AGAAAGAGCT CAGTCAGCTG GCCAGGCTGA CCGACGGTTA CTCTGGCAGC 
GATCTCACCT CTCTGGCTAA AGACGCTGCT CTCGGCCCGA TACGAGAGCT GAAGCCGGAG 
CAGGTGAGGA ACATGTCAGC CCATGAGATG CGTGACATTC GCATCTCTGA TTTCCTGGAG 
TCTCTGAAGC GGATCAAGCG GAGCGTCAGT CCGCAGACCT TGGATCAGTA CGTGCGCTGG 
AACCGCGAGT ACGGAGACAC GACTGGCGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998080.2
CDS	119..1831
Translation	MNSGHKARLRGGRACGPVSDGSARGNRLLFYTRSLSRVPEWLLRVLLLLLRWLFQPIRRAMAARAKECGPDGSEETGERIRNYHKQAFEFISVALQIDEDEKGDKQKAVQWYRKGIAELEKGIQIQVTGAGEKADRARKLQDKMITNLSMAEDRLKLLGNLLSQSPAESSSDDSFYSFSNGNLRPAPASGAVSKKKDTLTITNQTSLRPKNPPKSTPNASGLNCTPSAAQSSRTGPQNNQKGPTVKGKNNVKASTTATASPQRKRDMKNFKNVDSKLASLILNEIVDSGSVVRFDDIAGQDLAKQALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAMESNATFFNISAATLTSKYVGEGEKLVRALFAVARELQPSIIFIDEIDSLLCERREGEHDASRRLKTEFLIEFDGVQSGGDERVLVMGATNRPQELDEAVLRRFAKRIYVALPTEETRLKLLKNLLSKHRNPLSQKELSQLARLTDGYSGSDLTSLAKDAALGPIRELKPEQVRNMSAHEMRDIRISDFLESLKRIKRSVSPQTLDQYVRWNREYGDTTGV

Target ORF information:

RefSeq Version	NM_212915.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio spastin (spast), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212915.2

ORF Insert Sequence:

ATGAATTCTG GGCACAAAGC GAGACTCAGA GGAGGACGCG CTTGCGGTCC GGTGTCTGAC 
GGGAGCGCGC GGGGGAACCG GTTGCTCTTT TACACCCGCT CCCTGTCCAG GGTCCCGGAG 
TGGTTGCTGC GGGTGCTGCT GCTGCTTCTT CGGTGGCTGT TTCAGCCGAT TCGACGAGCC 
ATGGCGGCCC GGGCCAAAGA GTGCGGCCCC GACGGCAGTG AAGAGACCGG AGAGCGGATC 
CGAAACTACC ACAAACAAGC CTTCGAGTTC ATTTCTGTGG CTCTGCAGAT CGACGAGGAC 
GAGAAAGGTG ATAAGCAGAA GGCGGTGCAG TGGTACAGAA AAGGCATCGC TGAACTGGAG 
AAGGGCATCC AGATTCAGGT CACTGGAGCT GGCGAGAAGG CAGACAGAGC CAGAAAACTC 
CAGGACAAAA TGATCACCAA CCTGTCCATG GCCGAAGACC GGCTGAAGCT TCTGGGCAAT 
CTGCTTTCAC AGTCTCCAGC AGAGAGCAGC TCTGATGATT CTTTCTACTC TTTCTCCAAT 
GGTAATCTGC GTCCAGCTCC AGCCAGTGGA GCAGTCTCCA AGAAGAAGGA CACTCTCACA 
ATCACAAATC AAACTTCTCT GCGGCCCAAA AATCCTCCCA AATCTACCCC GAATGCATCA 
GGTCTGAACT GCACTCCCTC AGCAGCTCAG AGCAGCAGGA CCGGCCCTCA AAACAATCAG 
AAGGGGCCCA CAGTCAAAGG CAAAAATAAT GTTAAAGCCA GTACCACTGC CACTGCGTCA 
CCTCAAAGGA AAAGAGACAT GAAGAACTTC AAAAATGTGG ACAGCAAACT GGCAAGCCTC 
ATCCTCAACG AGATCGTGGA CAGCGGGTCT GTGGTGAGGT TTGATGATAT AGCAGGACAG 
GATTTGGCCA AGCAGGCGCT GCAGGAAATC GTCATCCTGC CTGCCCTACG TCCAGAGTTG 
TTTACTGGTC TCCGGGCTCC AGCTAGAGGA CTCCTGCTCT TCGGCCCTCC TGGTAATGGG 
AAAACCATGC TGGCGAAAGC CGTCGCAATG GAATCGAATG CTACTTTCTT CAACATCAGC 
GCTGCCACTC TCACCTCCAA ATATGTGGGA GAGGGAGAGA AGCTGGTTCG AGCTCTGTTT 
GCGGTCGCTC GAGAGCTGCA GCCGTCGATT ATCTTCATCG ATGAGATCGA CAGTCTGCTG 
TGTGAGAGGA GGGAAGGCGA ACACGATGCC AGCAGACGAC TCAAAACAGA GTTTCTCATC 
GAGTTTGATG GAGTGCAGTC AGGTGGAGAT GAGCGTGTGT TAGTGATGGG AGCCACTAAC 
AGGCCTCAGG AGCTGGACGA GGCTGTTCTC AGGAGATTTG CTAAACGCAT TTATGTGGCT 
TTACCTACAG AGGAGACTCG ACTGAAGCTG CTGAAGAATC TCCTCAGTAA ACACAGAAAT 
CCTCTGTCTC AGAAAGAGCT CAGTCAGCTG GCCAGGCTGA CCGACGGTTA CTCTGGCAGC 
GATCTCACCT CTCTGGCTAA AGACGCTGCT CTCGGCCCGA TACGAGAGCT GAAGCCGGAG 
CAGGTGAGGA ACATGTCAGC CCATGAGATG CGTGACATTC GCATCTCTGA TTTCCTGGAG 
TCTCTGAAGC GGATCAAGCG GAGCGTCAGT CCGCAGACCT TGGATCAGTA CGTGCGCTGG 
AACCGCGAGT ACGGAGACAC GACTGGCGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary recruitment of flexible Esrp-dependent splicing programs into diverse embryonic morphogenetic processes.
Nature communications8(1)1799(2017 Nov)
Burguera D,Marquez Y,Racioppi C,Permanyer J,Torres-M?ndez A,Esposito R,Albuixech-Crespo B,Fanlo L,D'Agostino Y,Gohr A,Navas-Perez E,Riesgo A,Cuomo C,Benvenuto G,Christiaen LA,Mart? E,D'Aniello S,Spagnuolo A,Ristoratore F,Arnone MI,Garcia-Fern?ndez J,Irimia M

Conserved pharmacological rescue of hereditary spastic paraplegia-related phenotypes across model organisms.
Human molecular genetics25(6)1088-99(2016 Mar)
Julien C,Lissouba A,Madabattula S,Fardghassemi Y,Rosenfelt C,Androschuk A,Strautman J,Wong C,Bysice A,O'sullivan J,Rouleau GA,Drapeau P,Parker JA,Bolduc FV

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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SPAST cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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