dact1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dact1
Entrez Gene ID	405799
Full Name	dishevelled-binding antagonist of beta-catenin 1
Synonyms	dapper1,dpr1,frd1,frodo1
General protein information	Preferred Names dishevelled-binding antagonist of beta-catenin 1 Names dapper homolog 1 dapper homolog 1, antagonist of beta-catenin dishevelled-interacting protein frodo 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_214731.3	NP_999896.3	dapper homolog 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dact1	NP_001177395.1
Danio rerio (zebrafish)	dact1	NP_999896.2
Homo sapiens (human)	DACT1	NP_057735.2
	DACT1	XP_001092593.1
Xenopus tropicalis (tropical clawed frog)	dact1	NP_001007950.1
Rattus norvegicus (Norway rat)	Dact1	XP_576044.1
Gallus gallus (chicken)	DACT1	NP_001038157.1
Pan troglodytes (chimpanzee)	DACT1	XP_001165895.1
Canis lupus familiaris (dog)	DACT1	XP_003435129.1
Bos taurus (cattle)	DACT1	XP_002691006.1

Gene Ontology
Bibliography

Related articles in PubMed

Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.
Zhou S, Zhao H, Thongda W, Zhang D, Su B, Yu D, Peatman E, Li C
Fish & shellfish immunology49324-35(2016 Feb)

Retinoic acid negatively regulates dact3b expression in the hindbrain of zebrafish embryos.
Mandal A, Waxman J
Gene expression patterns : GEP16(2)122-9(2014 Nov)

Maternally inherited npm2 mRNA is crucial for egg developmental competence in zebrafish.
Bouleau A, Desvignes T, Traverso JM, Nguyen T, Chesnel F, Fauvel C, Bobe J
Biology of reproduction91(2)43(2014 Aug)

Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition.
Lee MT, Bonneau AR, Takacs CM, Bazzini AA, DiVito KR, Fleming ES, Giraldez AJ
Nature503(7476)360-4(2013 Nov)

Evolutionary plasticity of segmentation clock networks.
Krol AJ, Roellig D, Dequ?ant ML, Tassy O, Glynn E, Hattem G, Mushegian A, Oates AC, Pourqui? O
Development (Cambridge, England)138(13)2783-92(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

target of beta-catenin and/or an unknown downstream effector in development
Title: Regulation of the early expression patterns of the zebrafish Dishevelled-interacting proteins Dapper1 and Dapper2.

two Dvl-associated paralogs, Dpr1 and Dpr2, participate in distinct Wnt-dependent developmental processes
Title: Zebrafish Dapper1 and Dapper2 play distinct roles in Wnt-mediated developmental processes.

frd1 is expressed in the developing brain and mesoderm.
Title: Two Frodo/Dapper homologs are expressed in the developing brain and mesoderm of zebrafish.

The following dact1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dact1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa40818	NM_214731.3 Latest version!	Danio rerio dishevelled-binding antagonist of beta-catenin 1 (dact1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dact1 ( NM_214731.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_999896.3 Danio rerio dishevelled-binding antagonist of beta-catenin 1 (dact1), mRNA.

Price & Availability↑

CloneID	ODa40818
Clone ID Related Accession (Same CDS sequence)	NM_214731.3
Accession Version	NM_214731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Danio rerio(zebrafish)
Product	dapper homolog 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU179699.7, EH591074.1, CABZ01005680.1 and EH608560.1. On Jun 25, 2015 this sequence version replaced NM_214731.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC127382.1, GFIL01008241.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACGAGT GGTCAGAGAT GCTGGTGTGC AGGGATTATT GCGCGCTCAT GATGCGGGAT 
CGGAAGGAAT GCGACGCGCG CTGCTGTCGG GACGGCGAAG GGGAGCGGGT TCGAGCCCGG 
GAGCGCTTGG ACGCCACTGT CGCGGGACTG ACCGAGCTGG AGTACTTGAG GCAGAGGCAG 
GAGCTGCTGG TCAGGAGTGT GTTGAGCTGT CCAGAGGCTG CAGGACAAGA CAAACCCTGC 
CTGACAGTGG ACGACAGCTA TCTGAACACT GAAGAGAAAC TTCTGGAGGA GAATATTTTG 
TTGCTTAGGA AACAGTTGAA CTGCCTCAGG AGGCGAGACG CAGGTTTGAT TAACCAGCTC 
CAGGAATTAG ACCGACAGAT CAGTGACCTG CGGCTGGACA CTGAGACTTC GCATGAACAT 
GTGGAAACCG ACAGCCGGCC AAGCTCTGGC TTTTACGACT TAAGCGACGG TGCTTCAGGA 
TCCCTCTCCA ATTCCTCCAA CTCTGTGTTC AGCGAGTGTT TGTCCAGTTG TCGTTCCACC 
ACCTGCCTGT GCACCCCCCT CGACACCTCG CTCTGTGCCT CAGAGGGAAG GCTTAAGCCT 
GCAGATGATC CGGGCAGTTG TGCCGAGTGT GATTGCCATT GTGAAGACTC CAGTTCTGGA 
ACAGTTCGCA GGTCTCTTTC TGCTTCCTAC TCCCCTTCCC CGGATAGCTC CTGCGATGGC 
ATTTCCAAAT TTCACTGTGA CCTAATTGCC AAAAATGGTA ATGATGTTTA CCGGTACCCC 
AGCCCTCTCC ATGCGGTGGC TGTCCAAAGC CCCGTTTTTA TCCAGTCGAT GACAAGTCAC 
CTAAAGGACG ATTGTAACTT CTCCAAGCCT GGGGAAGCAT TGAATGATGA ACAGAAACCC 
GAGCAGGTTG TAGGCTCCCA GACATCATCT TGGCATGCCT CTCAGATGCT TCCAAACAAA 
AAACTGGATA GTTACATCTT CGGGCTACTT CAGAGGAGGG CACAGCCCTT GAGGACCAAC 
AAACCCAGAA CAAGCATCAA TACTGACCCC TCCAAAAGCA TTTTAAGGCA GGCTAGTCTT 
TGTTCACGGG CTCCTGCTTC TGTTCAGGCC CAGCAATGGA CCTCTGACCT TAAACCAAAC 
TGGCAGACAT GTTTACAGGA TGCATCAGCA ACTAGCACTG AACCAAGCAC AGCTTCACCC 
CAAAGACAGT GGTCTGCTGA GTCTAAGGGT GGAACTCCTC AAAATGGAGC ATACTTGTCT 
TCAAGTCAGC CACAGAACAG TTATTCAACC ACAAATGAAA ACACTAATTG TCTGTTGAAG 
AAGAAAGTTT CTGGGACCAG TAAAGGTCAG CTGTTAAGTG CTACACCGAA AGACTGCCCG 
GATTTGGGCA GCCCAAAGGC TGTTTCCTCT CCTAAACTCA ACAAGCATTG TTTTTACAAT 
GTGGAGGACA ATAAAACAGG CCAAGCAATG AAAGCAAGTC CTCTAAAGAG GAGTCCTAAG 
ACTCAAACTT CGCTCAGTTG TGGTAAAGAC AGCGAGCAGT TGGCTCAAGA GTTGGTAAGC 
CTCGGTTCTT CTTCACAAAG TCAAGATGAG GGAGGTCCTC TGGTCAGTGC CCAGTATATT 
CCTGCTCAGA AACAGAACGT AAATCTTCAA AAGGGTGGCA CCAAGAACAT CAAGATTGGG 
AAAGTGAAAA ATTCTGCTAG TTCAAAAAGT AGACCTCAAG TGTTCGAGCA TGTTTCAGAG 
ACTGTCCGTG ACAAGCATCG AACAGGATCT AGGAGGACTC GTCAAGTAGA TGAAGTCCAC 
CACTTGCACA AATCCTCCAA GAAGGCCTCG GCAAAAACCA AGAGAATTCC TGCCTCCATC 
CCTGAAGGAC GAATCCTGGA GAGGCACACC AGTTCCTCAG GGGCTCGATC CTCTGCCCAG 
AGACACCATG GTCATCACCG ACATCACGAG GCAGTGTTGG CTAAACCTAA ATACAAGCGC 
AATGATTTCC ACCGGCGCCC GAGGGGTCTC CATGAGATCC CCTATGAGGA GGCTTATAGA 
AGGGCTCACC GCAGGCAAAA ACGTGAAATG CTGAGCCACA TGTACCTGCC GTCTAATGCA 
CACTACACCA GCCCCTACGC CTATGTTGGC AGTGACTCTG AGTATTCAGC AGAGTGCGCC 
TCCCTTTTCC ATTCCACCAT TTTGGACACA AGCGAGGACG AGCGCAGCAA CTACACTACC 
AACTGTTTTG GGGACAGTGA GTCGAGTGCT AGCGAGGCTG ACTATGTAGC CGAAAGCAGC 
ACTAGCAGTG ACTCTGAGGG CAGCGCTGGA GTCAATTGGC GCCAGATCAG CCAGGCAGGC 
TCAGGATCCC ATGGAATGAC GTCAGCACAG GCGAAGGCTT TTGTCAAAAT CAAGGCTTCC 
CATAACTTGA AGAAGAAGAT CTTGCGTTTC CGATCAGGCT CCTTAAAACT CATGACTACA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999896.3
CDS	300..2765
Translation	MNEWSEMLVCRDYCALMMRDRKECDARCCRDGEGERVRARERLDATVAGLTELEYLRQRQELLVRSVLSCPEAAGQDKPCLTVDDSYLNTEEKLLEENILLLRKQLNCLRRRDAGLINQLQELDRQISDLRLDTETSHEHVETDSRPSSGFYDLSDGASGSLSNSSNSVFSECLSSCRSTTCLCTPLDTSLCASEGRLKPADDPGSCAECDCHCEDSSSGTVRRSLSASYSPSPDSSCDGISKFHCDLIAKNGNDVYRYPSPLHAVAVQSPVFIQSMTSHLKDDCNFSKPGEALNDEQKPEQVVGSQTSSWHASQMLPNKKLDSYIFGLLQRRAQPLRTNKPRTSINTDPSKSILRQASLCSRAPASVQAQQWTSDLKPNWQTCLQDASATSTEPSTASPQRQWSAESKGGTPQNGAYLSSSQPQNSYSTTNENTNCLLKKKVSGTSKGQLLSATPKDCPDLGSPKAVSSPKLNKHCFYNVEDNKTGQAMKASPLKRSPKTQTSLSCGKDSEQLAQELVSLGSSSQSQDEGGPLVSAQYIPAQKQNVNLQKGGTKNIKIGKVKNSASSKSRPQVFEHVSETVRDKHRTGSRRTRQVDEVHHLHKSSKKASAKTKRIPASIPEGRILERHTSSSGARSSAQRHHGHHRHHEAVLAKPKYKRNDFHRRPRGLHEIPYEEAYRRAHRRQKREMLSHMYLPSNAHYTSPYAYVGSDSEYSAECASLFHSTILDTSEDERSNYTTNCFGDSESSASEADYVAESSTSSDSEGSAGVNWRQISQAGSGSHGMTSAQAKAFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	NM_214731.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dishevelled-binding antagonist of beta-catenin 1 (dact1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214731.3

ORF Insert Sequence:

ATGAACGAGT GGTCAGAGAT GCTGGTGTGC AGGGATTATT GCGCGCTCAT GATGCGGGAT 
CGGAAGGAAT GCGACGCGCG CTGCTGTCGG GACGGCGAAG GGGAGCGGGT TCGAGCCCGG 
GAGCGCTTGG ACGCCACTGT CGCGGGACTG ACCGAGCTGG AGTACTTGAG GCAGAGGCAG 
GAGCTGCTGG TCAGGAGTGT GTTGAGCTGT CCAGAGGCTG CAGGACAAGA CAAACCCTGC 
CTGACAGTGG ACGACAGCTA TCTGAACACT GAAGAGAAAC TTCTGGAGGA GAATATTTTG 
TTGCTTAGGA AACAGTTGAA CTGCCTCAGG AGGCGAGACG CAGGTTTGAT TAACCAGCTC 
CAGGAATTAG ACCGACAGAT CAGTGACCTG CGGCTGGACA CTGAGACTTC GCATGAACAT 
GTGGAAACCG ACAGCCGGCC AAGCTCTGGC TTTTACGACT TAAGCGACGG TGCTTCAGGA 
TCCCTCTCCA ATTCCTCCAA CTCTGTGTTC AGCGAGTGTT TGTCCAGTTG TCGTTCCACC 
ACCTGCCTGT GCACCCCCCT CGACACCTCG CTCTGTGCCT CAGAGGGAAG GCTTAAGCCT 
GCAGATGATC CGGGCAGTTG TGCCGAGTGT GATTGCCATT GTGAAGACTC CAGTTCTGGA 
ACAGTTCGCA GGTCTCTTTC TGCTTCCTAC TCCCCTTCCC CGGATAGCTC CTGCGATGGC 
ATTTCCAAAT TTCACTGTGA CCTAATTGCC AAAAATGGTA ATGATGTTTA CCGGTACCCC 
AGCCCTCTCC ATGCGGTGGC TGTCCAAAGC CCCGTTTTTA TCCAGTCGAT GACAAGTCAC 
CTAAAGGACG ATTGTAACTT CTCCAAGCCT GGGGAAGCAT TGAATGATGA ACAGAAACCC 
GAGCAGGTTG TAGGCTCCCA GACATCATCT TGGCATGCCT CTCAGATGCT TCCAAACAAA 
AAACTGGATA GTTACATCTT CGGGCTACTT CAGAGGAGGG CACAGCCCTT GAGGACCAAC 
AAACCCAGAA CAAGCATCAA TACTGACCCC TCCAAAAGCA TTTTAAGGCA GGCTAGTCTT 
TGTTCACGGG CTCCTGCTTC TGTTCAGGCC CAGCAATGGA CCTCTGACCT TAAACCAAAC 
TGGCAGACAT GTTTACAGGA TGCATCAGCA ACTAGCACTG AACCAAGCAC AGCTTCACCC 
CAAAGACAGT GGTCTGCTGA GTCTAAGGGT GGAACTCCTC AAAATGGAGC ATACTTGTCT 
TCAAGTCAGC CACAGAACAG TTATTCAACC ACAAATGAAA ACACTAATTG TCTGTTGAAG 
AAGAAAGTTT CTGGGACCAG TAAAGGTCAG CTGTTAAGTG CTACACCGAA AGACTGCCCG 
GATTTGGGCA GCCCAAAGGC TGTTTCCTCT CCTAAACTCA ACAAGCATTG TTTTTACAAT 
GTGGAGGACA ATAAAACAGG CCAAGCAATG AAAGCAAGTC CTCTAAAGAG GAGTCCTAAG 
ACTCAAACTT CGCTCAGTTG TGGTAAAGAC AGCGAGCAGT TGGCTCAAGA GTTGGTAAGC 
CTCGGTTCTT CTTCACAAAG TCAAGATGAG GGAGGTCCTC TGGTCAGTGC CCAGTATATT 
CCTGCTCAGA AACAGAACGT AAATCTTCAA AAGGGTGGCA CCAAGAACAT CAAGATTGGG 
AAAGTGAAAA ATTCTGCTAG TTCAAAAAGT AGACCTCAAG TGTTCGAGCA TGTTTCAGAG 
ACTGTCCGTG ACAAGCATCG AACAGGATCT AGGAGGACTC GTCAAGTAGA TGAAGTCCAC 
CACTTGCACA AATCCTCCAA GAAGGCCTCG GCAAAAACCA AGAGAATTCC TGCCTCCATC 
CCTGAAGGAC GAATCCTGGA GAGGCACACC AGTTCCTCAG GGGCTCGATC CTCTGCCCAG 
AGACACCATG GTCATCACCG ACATCACGAG GCAGTGTTGG CTAAACCTAA ATACAAGCGC 
AATGATTTCC ACCGGCGCCC GAGGGGTCTC CATGAGATCC CCTATGAGGA GGCTTATAGA 
AGGGCTCACC GCAGGCAAAA ACGTGAAATG CTGAGCCACA TGTACCTGCC GTCTAATGCA 
CACTACACCA GCCCCTACGC CTATGTTGGC AGTGACTCTG AGTATTCAGC AGAGTGCGCC 
TCCCTTTTCC ATTCCACCAT TTTGGACACA AGCGAGGACG AGCGCAGCAA CTACACTACC 
AACTGTTTTG GGGACAGTGA GTCGAGTGCT AGCGAGGCTG ACTATGTAGC CGAAAGCAGC 
ACTAGCAGTG ACTCTGAGGG CAGCGCTGGA GTCAATTGGC GCCAGATCAG CCAGGCAGGC 
TCAGGATCCC ATGGAATGAC GTCAGCACAG GCGAAGGCTT TTGTCAAAAT CAAGGCTTCC 
CATAACTTGA AGAAGAAGAT CTTGCGTTTC CGATCAGGCT CCTTAAAACT CATGACTACA 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Galectins in channel catfish, Ictalurus punctatus: Characterization and expression profiling in mucosal tissues.
Fish & shellfish immunology49324-35(2016 Feb)
Zhou S,Zhao H,Thongda W,Zhang D,Su B,Yu D,Peatman E,Li C

Retinoic acid negatively regulates dact3b expression in the hindbrain of zebrafish embryos.
Gene expression patterns : GEP16(2)122-9(2014 Nov)
Mandal A,Waxman J

Maternally inherited npm2 mRNA is crucial for egg developmental competence in zebrafish.
Biology of reproduction91(2)43(2014 Aug)
Bouleau A,Desvignes T,Traverso JM,Nguyen T,Chesnel F,Fauvel C,Bobe J

Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition.
Nature503(7476)360-4(2013 Nov)
Lee MT,Bonneau AR,Takacs CM,Bazzini AA,DiVito KR,Fleming ES,Giraldez AJ

Evolutionary plasticity of segmentation clock networks.
Development (Cambridge, England)138(13)2783-92(2011 Jul)
Krol AJ,Roellig D,Dequ?ant ML,Tassy O,Glynn E,Hattem G,Mushegian A,Oates AC,Pourqui? O

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dact1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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