six1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	six1b
Entrez Gene ID	404627
Full Name	SIX homeobox 1b
Synonyms	six1,zgc:77345
General protein information	Preferred Names SIX homeobox 1b Names homeobox protein six1b homeobox protein six1a homeodomain transcription factor six1 sine oculis homeobox homolog 1 sine oculis homeobox homolog 1a sine oculis homeobox homolog 1b six1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207095.1	NP_996978.1	homeobox protein six1b

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SIX1	XP_547841.3
Mus musculus (house mouse)	Six1	NP_033215.2
Gallus gallus (chicken)	SIX1	NP_001038150.1
Danio rerio (zebrafish)	six1b	NP_996978.1
Pan troglodytes (chimpanzee)	SIX1	XP_509988.2
	SIX1	XP_001096585.1
Rattus norvegicus (Norway rat)	Six1	NP_446211.1
Xenopus tropicalis (tropical clawed frog)	six1	NP_001093693.1
Homo sapiens (human)	SIX1	NP_005973.1
Bos taurus (cattle)	SIX1	XP_002691065.3
Drosophila melanogaster (fruit fly)	so	NP_476733.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011695	XP_320814.4

Gene Ontology
Bibliography

Related articles in PubMed

Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish.
Nakahara Y, Muto A, Hirabayashi R, Sakuma T, Yamamoto T, Kume S, Kikuchi Y
Genes to cells : devoted to molecular & cellular mechanisms21(5)492-504(2016 May)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate a new role for miR30a at a key node in the myogenic regulatory gene network through controlling Six1 expression.
Title: MicroRNA-30a regulates zebrafish myogenesis through targeting the transcription factor Six1.

Data show a mechanism for Six1a and Six1b in establishing the Pax7(+) cell derived part of the fast muscle and suggest new important roles for Six1 in the regulation of the Pax7(+) muscle cell population through pSmad1/5/8 signalling.
Title: Six1 regulates proliferation of Pax7-positive muscle progenitors in zebrafish.

six1 promotes inner hair cell fate and inhibits neuronal fate during development.
Title: The transcription factor six1 inhibits neuronal and promotes hair cell fate in the developing zebrafish (Danio rerio) inner ear.

zebrafish six1 is expressed during sensory organ development and myogenesis
Title: Expression of zebrafish six1 during sensory organ development and myogenesis.

The following six1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the six1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03628	NM_207095.1 Latest version!	Danio rerio SIX homeobox 1b (six1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

six1b ( NM_207095.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_996978.1 Danio rerio SIX homeobox 1b (six1b), mRNA.

Price & Availability↑

CloneID	ODa03628
Clone ID Related Accession (Same CDS sequence)	NM_207095.1
Accession Version	NM_207095.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Danio rerio(zebrafish)
Product	homeobox protein six1b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066396.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066396.1, AY254196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCAATGT TGCCTTCTTT CGGGTTTACG CAGGAGCAAG TCGCGTGTGT GTGTGAGGTG 
CTTCAACAAG GGGGGAACCT CGAGCGTCTC GGACGATTTC TGTGGTCTCT CCCGGCTTGC 
GATCATCTCC ACAAAAACGA AAGCGTCCTC AAAGCCAAGG CTGTCGTCGC CTTCCATCGT 
GGAAACTTCA GGGAACTTTA TAAGATACTA GAGAGCCACC AGTTCTCTCC GCACAACCAC 
CCGAAGCTGC AGCAGCTGTG GCTTAAGGCC CATTACATTG AGGCTGAGAA GCTGAGGGGG 
CGACCGCTGG GAGCCGTGGG GAAATACCGA GTGCGCAGGA AATTTCCTCT GCCTCGCACA 
ATATGGGACG GAGAGGAGAC CAGCTACTGT TTTAAGGAGA AGTCCCGCGG GGTGCTGCGA 
GAGTGGTACA CGCACAACCC CTATCCATCT CCTCGGGAAA AGAGGGAGCT GGCCGAGGCC 
ACAGGACTGA CTACCACACA AGTGAGCAAC TGGTTTAAAA ACAGACGGCA GAGGGATCGC 
GCAGCGGAGG CGAAAGAAAG GGAAAACAGC GAGAACAACA ACACGGGCGC TAACAAACAG 
AACCAGCTGT CACCGCTGGA CGGCGGGAAG TCCCTGATGT CGAGCTCGGA GGACGAATTC 
TCGCCCCCGC AGAGTCCTGA CCAGAACTCC GTGCTCCTGC TCCAGGGTAA TATGAGCCAC 
CCCGGCGCGT CCGCGTACTC CATGACCGGT CTCGGCGCCG CACAGTCGGT ACACGGCATG 
CAAGGACACC CACATCAGCT CCAGGATTCA TTACTGGGAC CTTTAACTTC AAGTCTAGTG 
GACCTAGGCT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996978.1
CDS	145..999
Translation	MSMLPSFGFTQEQVACVCEVLQQGGNLERLGRFLWSLPACDHLHKNESVLKAKAVVAFHRGNFRELYKILESHQFSPHNHPKLQQLWLKAHYIEAEKLRGRPLGAVGKYRVRRKFPLPRTIWDGEETSYCFKEKSRGVLREWYTHNPYPSPREKRELAEATGLTTTQVSNWFKNRRQRDRAAEAKERENSENNNTGANKQNQLSPLDGGKSLMSSSEDEFSPPQSPDQNSVLLLQGNMSHPGASAYSMTGLGAAQSVHGMQGHPHQLQDSLLGPLTSSLVDLGS

Target ORF information:

RefSeq Version	NM_207095.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio SIX homeobox 1b (six1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207095.1

ORF Insert Sequence:

ATGTCAATGT TGCCTTCTTT CGGGTTTACG CAGGAGCAAG TCGCGTGTGT GTGTGAGGTG 
CTTCAACAAG GGGGGAACCT CGAGCGTCTC GGACGATTTC TGTGGTCTCT CCCGGCTTGC 
GATCATCTCC ACAAAAACGA AAGCGTCCTC AAAGCCAAGG CTGTCGTCGC CTTCCATCGT 
GGAAACTTCA GGGAACTTTA TAAGATACTA GAGAGCCACC AGTTCTCTCC GCACAACCAC 
CCGAAGCTGC AGCAGCTGTG GCTTAAGGCC CATTACATTG AGGCTGAGAA GCTGAGGGGG 
CGACCGCTGG GAGCCGTGGG GAAATACCGA GTGCGCAGGA AATTTCCTCT GCCTCGCACA 
ATATGGGACG GAGAGGAGAC CAGCTACTGT TTTAAGGAGA AGTCCCGCGG GGTGCTGCGA 
GAGTGGTACA CGCACAACCC CTATCCATCT CCTCGGGAAA AGAGGGAGCT GGCCGAGGCC 
ACAGGACTGA CTACCACACA AGTGAGCAAC TGGTTTAAAA ACAGACGGCA GAGGGATCGC 
GCAGCGGAGG CGAAAGAAAG GGAAAACAGC GAGAACAACA ACACGGGCGC TAACAAACAG 
AACCAGCTGT CACCGCTGGA CGGCGGGAAG TCCCTGATGT CGAGCTCGGA GGACGAATTC 
TCGCCCCCGC AGAGTCCTGA CCAGAACTCC GTGCTCCTGC TCCAGGGTAA TATGAGCCAC 
CCCGGCGCGT CCGCGTACTC CATGACCGGT CTCGGCGCCG CACAGTCGGT ACACGGCATG 
CAAGGACACC CACATCAGCT CCAGGATTCA TTACTGGGAC CTTTAACTTC AAGTCTAGTG 
GACCTAGGCT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish.
Genes to cells : devoted to molecular & cellular mechanisms21(5)492-504(2016 May)
Nakahara Y,Muto A,Hirabayashi R,Sakuma T,Yamamoto T,Kume S,Kikuchi Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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six1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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