leo1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	leo1
Entrez Gene ID	404599
Full Name	LEO1 homolog, Paf1/RNA polymerase II complex component
Synonyms	zgc:77374
General protein information	Preferred Names LEO1 homolog, Paf1/RNA polymerase II complex component Names RNA polymerase-associated protein LEO1 Leo1, Paf1/RNA polymerase II complex component, homolog
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_207067.1	NP_996950.1	RNA polymerase-associated protein LEO1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LEO1	XP_535485.2
Rattus norvegicus (Norway rat)	Leo1	NP_001005548.1
Pan troglodytes (chimpanzee)	LEO1	NP_001233372.1
Gallus gallus (chicken)	LEO1	XP_004943851.1
Bos taurus (cattle)	LEO1	NP_001076073.1
Mus musculus (house mouse)	Leo1	NP_001034611.1
Danio rerio (zebrafish)	leo1	NP_996950.1
Homo sapiens (human)	LEO1	NP_620147.1
Xenopus tropicalis (tropical clawed frog)	leo1	XP_002936391.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Antagonistic regulation of PAF1C and p-TEFb is required for oligodendrocyte differentiation.
Kim S, Kim JD, Chung AY, Kim HS, Kim YS, Kim MJ, Koun S, Lee YM, Rhee M, Park HC, Huh TL
The Journal of neuroscience : the official journal of the Society for Neuroscience32(24)8201-7(2012 Jun)

The PAF1 complex differentially regulates cardiomyocyte specification.
Langenbacher AD, Nguyen CT, Cavanaugh AM, Huang J, Lu F, Chen JN
Developmental biology353(1)19-28(2011 May)

The PAF1 complex component Leo1 is essential for cardiac and neural crest development in zebrafish.
Nguyen CT, Langenbacher A, Hsieh M, Chen JN
Developmental biology341(1)167-75(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that antagonistic regulation of gene expression by PAF1Cand p-TEFb plays a crucial role in oligodendrocyte development in the CNS.
Title: Antagonistic regulation of PAF1C and p-TEFb is required for oligodendrocyte differentiation.

These results provide the first genetic evidence of the requirement for Leo1 in the development of the heart and neural crest cell populations.
Title: The PAF1 complex component Leo1 is essential for cardiac and neural crest development in zebrafish.

The following leo1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the leo1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37853	NM_207067.1 Latest version!	Danio rerio LEO1 homolog, Paf1/RNA polymerase II complex component (leo1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

leo1 ( NM_207067.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_996950.1 Danio rerio LEO1 homolog, Paf1/RNA polymerase II complex component (leo1), mRNA.

Price & Availability↑

CloneID	ODa37853
Clone ID Related Accession (Same CDS sequence)	NM_207067.1
Accession Version	NM_207067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Danio rerio(zebrafish)
Product	RNA polymerase-associated protein LEO1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066443.1. ##Evidence-Data-START## Transcript exon combination :: BC066443.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATA TGGACGAACT GTTCGGGAGC GATGGCGATA GCGACAATGA GCAACGAGAT 
TCCGGCTCTG GTTCTGGCTC TGATTCGGAT CATGAGAGAC CAAGATCTGC CAGCAACGCT 
TCTGGAAGCG AGAGTGCTCA GAGTGACCGA GATCATGATG ATGATGAAGA TGAAGATGGC 
GGAAAGCCTA GCAATAAAGA GCTGTTTGGA GACGACAGTG AAGATGAGCA CGGCAGCCAG 
CACAGCGGGA GCCAGCACAG CGGCAGCCGG CACAGCGGCA GCCGGCACAG CGGCAGCAGG 
CACAGTGGCA GTAGGCACAG TGGCAGCCAG CACAGCGGGA GCCAATCAGA GCGATCGGGC 
AACCAATCAG ACGCCACCAT GCACTCTGAC AATGAACACA GCATGTCTGA GGCCCATCGT 
GGTGAGCAGG ATGATGAGGA TGATGATGAC AGAGGCCACA GGTCAGACGT AGGCAGTCCA 
GCCTCAGGAG CAGGAAGCCG CAGGTCGGAC AGAGGAAGTG GAAGTCCAGG GTCAGAGGCG 
GGAAGTCCAC GCTCAGAAGC TGGAAGTGGT CATTCGGATC CTGGAACCCC ACATACAGAT 
GGAGAGGGGT CAGGGAAAGA TGCCCATTCT GGAGATGAGA AGTGGGGTGG AGATGGAAAA 
AGTGACCAGT CGGAGGACGA GGACAAACAG CAGAACTCTG ATGATGAGAG GGAGCGATCT 
GATGAAGAAG GGGAGAGGCA GAAATCAGAG TCGATTAAGG GCAGTGACAG CGAGGATGAT 
TTCACACGCA AGAAAAAGAA AAAGATTGCT TCGGACTCGG ACTCAGACTC TGATGCTGAA 
ACACAGGGTG GTAAGAAGCC TGCAGCAAAT GATCTTTTTG GGGAGGCAGA TGATATTTCG 
TCAGACAGCG ATGCAGAGAA ACCTCTCACA CCTGGCCAAC CATTGGATGC AGATGACGGA 
ATGGAGGGAG ATCAGCCTGA GGAAGAGCCA GCTCCAGAGA CGAGGATTGA AGTGGAGATA 
CCTAAAGTCA GCACAGACCT GGGCAGCGAT CTCTACTTCG TCAAATTACC CAACTTTCTC 
AGTGTGGAGC CCAGGCCGTT CGACCCTCAG TACTATGAGG ATGAGTTTGA GGATGAGGAG 
ATGTTAGACG AGGAAGGAAG AACTCGTCTA AAGCTAAAGG TGGAGAACAC CATCAGGTGG 
CGCTCTCGAC GGGATGAGGA GGGCAATGAA GTCAAGGAGA GCAATGCTAG GATTGTTAAA 
TGGTCTGACG GCAGCATGTC TCTGCACCTG GGGAACGAAG TGTTTGATGT GTATAAAGCT 
CCACTGCAGG GTGACCACAA TCACCTGTTC ATCCGCCAGG GCACAGGACT GCAGGGTCAG 
GCTGTGTTCA AGACCAAACT CACCTTCAGA CCTCACTCCA CAGACAGCGC CACACACAGG 
AAGATGACGC TCTCTCTTGC CGACCGCTGT TCAAAGACAC AGAAGATCCG AATTTTGCCC 
ATGGCTGGCA GAGACCCAGA GTCCCAGAGA AATGAGATGA TCAAGAAAGA AGAGGAGAGG 
TTACGTGCGT CCATCAGGCG CGAGTCCCAG CAGAGGCGCA TGAGAGAGAA ACAGCATCAA 
CGAGGTCTGA ACGCCGGCTA CCTGGAGCCT GACCGCTACG ATGAGGACGA AGAGGGTGAA 
GAGTCCATTA GCCTGGCTGC AATCAAGAGC AAATATAAGG GTGGAGGAGG TCTAAGGGAG 
GAACGAGCCA GGATCTATTC GTCAGACAGC GATGAAGGTT CTGATGAAGA CAAGGCCCAG 
CGACTGATGA AGGCCAAGAG ACTGGACAGC GATGAGGAGG GAGAGAACTC TGGAAAGAGG 
AAGGCAGAGG AAGATGAGGA ATCTGCTTCA AAGAAGCCCA AAAAGTATGT CATTAGTGAT 
GAAGAAGACG AGGACGGAGA TGGAGAACGC GATGGAGACC GAGAGAGAGG CGGGGATATG 
GATGGAGACG GAGATGGAGA TATGGAAGGA GATGGAGATG TGGACAGAGA CGGAGATATG 
GATGGAGATG GAGAAGGCGA TGGAGAGGGA GACGGAGAGG AAGACGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996950.1
CDS	3..2093
Translation	MADMDELFGSDGDSDNEQRDSGSGSGSDSDHERPRSASNASGSESAQSDRDHDDDEDEDGGKPSNKELFGDDSEDEHGSQHSGSQHSGSRHSGSRHSGSRHSGSRHSGSQHSGSQSERSGNQSDATMHSDNEHSMSEAHRGEQDDEDDDDRGHRSDVGSPASGAGSRRSDRGSGSPGSEAGSPRSEAGSGHSDPGTPHTDGEGSGKDAHSGDEKWGGDGKSDQSEDEDKQQNSDDERERSDEEGERQKSESIKGSDSEDDFTRKKKKKIASDSDSDSDAETQGGKKPAANDLFGEADDISSDSDAEKPLTPGQPLDADDGMEGDQPEEEPAPETRIEVEIPKVSTDLGSDLYFVKLPNFLSVEPRPFDPQYYEDEFEDEEMLDEEGRTRLKLKVENTIRWRSRRDEEGNEVKESNARIVKWSDGSMSLHLGNEVFDVYKAPLQGDHNHLFIRQGTGLQGQAVFKTKLTFRPHSTDSATHRKMTLSLADRCSKTQKIRILPMAGRDPESQRNEMIKKEEERLRASIRRESQQRRMREKQHQRGLNAGYLEPDRYDEDEEGEESISLAAIKSKYKGGGGLREERARIYSSDSDEGSDEDKAQRLMKAKRLDSDEEGENSGKRKAEEDEESASKKPKKYVISDEEDEDGDGERDGDRERGGDMDGDGDGDMEGDGDVDRDGDMDGDGEGDGEGDGEEDE

Target ORF information:

RefSeq Version	NM_207067.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio LEO1 homolog, Paf1/RNA polymerase II complex component (leo1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207067.1

ORF Insert Sequence:

ATGGCGGATA TGGACGAACT GTTCGGGAGC GATGGCGATA GCGACAATGA GCAACGAGAT 
TCCGGCTCTG GTTCTGGCTC TGATTCGGAT CATGAGAGAC CAAGATCTGC CAGCAACGCT 
TCTGGAAGCG AGAGTGCTCA GAGTGACCGA GATCATGATG ATGATGAAGA TGAAGATGGC 
GGAAAGCCTA GCAATAAAGA GCTGTTTGGA GACGACAGTG AAGATGAGCA CGGCAGCCAG 
CACAGCGGGA GCCAGCACAG CGGCAGCCGG CACAGCGGCA GCCGGCACAG CGGCAGCAGG 
CACAGTGGCA GTAGGCACAG TGGCAGCCAG CACAGCGGGA GCCAATCAGA GCGATCGGGC 
AACCAATCAG ACGCCACCAT GCACTCTGAC AATGAACACA GCATGTCTGA GGCCCATCGT 
GGTGAGCAGG ATGATGAGGA TGATGATGAC AGAGGCCACA GGTCAGACGT AGGCAGTCCA 
GCCTCAGGAG CAGGAAGCCG CAGGTCGGAC AGAGGAAGTG GAAGTCCAGG GTCAGAGGCG 
GGAAGTCCAC GCTCAGAAGC TGGAAGTGGT CATTCGGATC CTGGAACCCC ACATACAGAT 
GGAGAGGGGT CAGGGAAAGA TGCCCATTCT GGAGATGAGA AGTGGGGTGG AGATGGAAAA 
AGTGACCAGT CGGAGGACGA GGACAAACAG CAGAACTCTG ATGATGAGAG GGAGCGATCT 
GATGAAGAAG GGGAGAGGCA GAAATCAGAG TCGATTAAGG GCAGTGACAG CGAGGATGAT 
TTCACACGCA AGAAAAAGAA AAAGATTGCT TCGGACTCGG ACTCAGACTC TGATGCTGAA 
ACACAGGGTG GTAAGAAGCC TGCAGCAAAT GATCTTTTTG GGGAGGCAGA TGATATTTCG 
TCAGACAGCG ATGCAGAGAA ACCTCTCACA CCTGGCCAAC CATTGGATGC AGATGACGGA 
ATGGAGGGAG ATCAGCCTGA GGAAGAGCCA GCTCCAGAGA CGAGGATTGA AGTGGAGATA 
CCTAAAGTCA GCACAGACCT GGGCAGCGAT CTCTACTTCG TCAAATTACC CAACTTTCTC 
AGTGTGGAGC CCAGGCCGTT CGACCCTCAG TACTATGAGG ATGAGTTTGA GGATGAGGAG 
ATGTTAGACG AGGAAGGAAG AACTCGTCTA AAGCTAAAGG TGGAGAACAC CATCAGGTGG 
CGCTCTCGAC GGGATGAGGA GGGCAATGAA GTCAAGGAGA GCAATGCTAG GATTGTTAAA 
TGGTCTGACG GCAGCATGTC TCTGCACCTG GGGAACGAAG TGTTTGATGT GTATAAAGCT 
CCACTGCAGG GTGACCACAA TCACCTGTTC ATCCGCCAGG GCACAGGACT GCAGGGTCAG 
GCTGTGTTCA AGACCAAACT CACCTTCAGA CCTCACTCCA CAGACAGCGC CACACACAGG 
AAGATGACGC TCTCTCTTGC CGACCGCTGT TCAAAGACAC AGAAGATCCG AATTTTGCCC 
ATGGCTGGCA GAGACCCAGA GTCCCAGAGA AATGAGATGA TCAAGAAAGA AGAGGAGAGG 
TTACGTGCGT CCATCAGGCG CGAGTCCCAG CAGAGGCGCA TGAGAGAGAA ACAGCATCAA 
CGAGGTCTGA ACGCCGGCTA CCTGGAGCCT GACCGCTACG ATGAGGACGA AGAGGGTGAA 
GAGTCCATTA GCCTGGCTGC AATCAAGAGC AAATATAAGG GTGGAGGAGG TCTAAGGGAG 
GAACGAGCCA GGATCTATTC GTCAGACAGC GATGAAGGTT CTGATGAAGA CAAGGCCCAG 
CGACTGATGA AGGCCAAGAG ACTGGACAGC GATGAGGAGG GAGAGAACTC TGGAAAGAGG 
AAGGCAGAGG AAGATGAGGA ATCTGCTTCA AAGAAGCCCA AAAAGTATGT CATTAGTGAT 
GAAGAAGACG AGGACGGAGA TGGAGAACGC GATGGAGACC GAGAGAGAGG CGGGGATATG 
GATGGAGACG GAGATGGAGA TATGGAAGGA GATGGAGATG TGGACAGAGA CGGAGATATG 
GATGGAGATG GAGAAGGCGA TGGAGAGGGA GACGGAGAGG AAGACGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Antagonistic regulation of PAF1C and p-TEFb is required for oligodendrocyte differentiation.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(24)8201-7(2012 Jun)
Kim S,Kim JD,Chung AY,Kim HS,Kim YS,Kim MJ,Koun S,Lee YM,Rhee M,Park HC,Huh TL

The PAF1 complex differentially regulates cardiomyocyte specification.
Developmental biology353(1)19-28(2011 May)
Langenbacher AD,Nguyen CT,Cavanaugh AM,Huang J,Lu F,Chen JN

The PAF1 complex component Leo1 is essential for cardiac and neural crest development in zebrafish.
Developmental biology341(1)167-75(2010 May)
Nguyen CT,Langenbacher A,Hsieh M,Chen JN

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leo1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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