egf cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	egf
Entrez Gene ID	403045
Full Name	epidermal growth factor
General protein information	Preferred Names epidermal growth factor Names pro-epidermal growth factor
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017359001.1	XP_017214490.1	pro-epidermal growth factor isoform X1
XM_005157314.3	XP_005157371.1	pro-epidermal growth factor isoform X2
XM_021480997.1	XP_021336672.1	pro-epidermal growth factor isoform X2
NM_001365528.1	NP_001352457.1	pro-epidermal growth factor isoform 2 precursor
XM_005157314.4	XP_005157371.1	pro-epidermal growth factor isoform X1
NM_205731.1	NP_991294.1	pro-epidermal growth factor
XM_021480998.1	XP_021336673.1	pro-epidermal growth factor isoform X3
NM_001365527.1	NP_001352456.1	pro-epidermal growth factor isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	EGF	NP_001954.2
Bos taurus (cattle)	EGF	XP_002688149.2
Mus musculus (house mouse)	Egf	NP_034243.2
	EGF	XP_001088957.1
Rattus norvegicus (Norway rat)	Egf	NP_036974.1
Canis lupus familiaris (dog)	EGF	NP_001003094.1
Danio rerio (zebrafish)	egf	NP_991294.1
Pan troglodytes (chimpanzee)	EGF	XP_517395.2
Gallus gallus (chicken)	EGF	NP_001001292.1
Xenopus tropicalis (tropical clawed frog)	egf	NP_001106478.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypersensitive assessment of aryl hydrocarbon receptor transcriptional activity using a novel truncated
Luo JJ, Su DS, Xie SL, Liu Y, Liu P, Yang XJ, Pei DS
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2018 Jan)

Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs.
Hui SP, Sheng DZ, Sugimoto K, Gonzalez-Rajal A, Nakagawa S, Hesselson D, Kikuchi K
Developmental cell43(6)659-672.e5(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Zebrafish scube1 (signal peptide-CUB (complement protein C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor) domain-containing protein 1) is involved in primitive hematopoiesis
Title: Zebrafish scube1 (signal peptide-CUB (complement protein C1r/C1s, Uegf, and Bmp1)-EGF (epidermal growth factor) domain-containing protein 1) is involved in primitive hematopoiesis.

These results suggest that there is an EGF signaling network in the zebrafish ovarian follicle, and the functionality of this network is self-regulated by its own members.
Title: Differential regulation of betacellulin and heparin-binding EGF-like growth factor in cultured zebrafish ovarian follicle cells by EGF family ligands.

EGF is likely a potential paracrine/juxtacrine factor from the oocytes to regulate the function of the follicle cells.
Title: Cloning of epidermal growth factor (EGF) and EGF receptor from the zebrafish ovary: evidence for EGF as a potential paracrine factor from the oocyte to regulate activin/follistatin system in the follicle cells.

The following egf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the egf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00584	NM_205731.1 Latest version!	Danio rerio epidermal growth factor (egf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa19813	NM_001365527.1 Latest version!	Danio rerio epidermal growth factor (egf), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa40761	NM_001365528.1 Latest version!	Danio rerio epidermal growth factor (egf), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa148729	XM_021480998.1 Latest version!	Danio rerio epidermal growth factor (egf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ODa19813	XM_005157314.4 Latest version!	Danio rerio epidermal growth factor (egf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa40761	XM_021480997.1 Latest version!	Danio rerio epidermal growth factor (egf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
ODa54902	XM_017359001.1 Latest version!	Danio rerio epidermal growth factor (egf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
ODa19813	XM_005157314.3	Danio rerio epidermal growth factor (egf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

egf ( NM_205731.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991294.1 Danio rerio epidermal growth factor (egf), mRNA.

Price & Availability↑

CloneID	ODa00584
Clone ID Related Accession (Same CDS sequence)	NM_205731.1
Accession Version	NM_205731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	pro-epidermal growth factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY332224.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY332224.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAACCTCG ACGGAGGGGA CCGGAGAAGA ATCGTTTCTA GGGCGGGAAG GTCCATTTTG 
TTGGACTTTC ACTTAAGTGA GTGGACAATG TTTTGGGCGG ACACACAAAC AGGACAAATC 
AGCAGAGCAG GACTAGATGG AACACGAAGA CAGAAGCTTC TCTCCTCAGT GAAGGGAATC 
ACAGGCCTGG TTGTAGACTG GATTGAAAAC TCAGTCCTCT GGAGCAATGC AGATGAAGGA 
ACGATACAGA GAATGGACAC AGATGGCAAG AACGACAGAG TTGTCTTGAG AAATCTGTCT 
CAGCCCAGAG GTGTTGTTGT TGATCCAAAT GAAAGATATA TTTTCTGGTT GTCAGATGGA 
GCAACTTCCA GCATCCAGCG ATCAGACATG ACAGGTAAAA TGATGAGCAC AGTGCTGAAA 
GTAGCGGACA GACTGAAGGT TCTGGCTGTT GACCACCGGG ACAGGAGACT TTTCTGGGTT 
CAGCAAGGCC AAAGAGGACA CACTGCTATG GGCTCCTGCA ACTATGATGG AAACATCATC 
AATGTCTTTA ATCAGCAATT CAGACCTCAA TCTCTGAGAA TGACGATTTT CTTGGATTAT 
GTGTATCTGT CAGACTCAAA GTCAAAAACA ATAACTCGAT TGAACAAGTA CGCAGGGGGT 
CGACAAGAGA ATGTCAGTTC TCAAAGGATG CCACATTCCC CTGCTGATGT TAAAGTGGTG 
CATCCCATCA ATCAACCGGT GGTGGAAACT CCTTTTACAC CCGGCTGCAG TCGACACACA 
GGAGAGTGTG TGAAAGTGTG CTCCAGTCAC ACTGACACTG GTCTCTGCGG CTGCAAGGAT 
GGATTTACCC TCAGTAAACA TGGAAACATC TGTGAAGATG TCAATGAATG TTCCCTATGG 
AATCACGGCT GCTCTCTGGG ATGCGAGAAC GTTCCGGGCT CTTACTTCTG CACCTGTCCT 
GAGGGTTATC TGTTACTGCC CGATTTAAAG ACCTGCCAAG AGAACAAGCC TTGTGTGGGG 
AAAGCTGTGG ATTGTGATCA TGCATGTGTT CACACAGCAC AAGGAGATAT GTGTGTGTGT 
CCTGAAGGAT CCCTACTCAA TCCTGATGGC CAATCTTGTA CAGGCTGTTT GTCTGCAGAT 
CGTGGCGGCT GCAGTCAGAT GTGTGTGACT TTATATCCAG GCCGGTGGGT TTGTGAATGC 
CATCCTGGAT ACCAAATACA GCCTGATGGA AAACACTGCG CTGCCACTGG ACCTCCAGCG 
GACCTGTTGT TTGCCAATAT AGTGGATTTA AGGAAGATTA ACACGGATGG CAAACTGAGC 
ATAAAACTGC TGGAAAAACC AGGAGGCAAT ATTACAGCAG TGGACTATGA TCCAGTGACG 
AACAAGGTGT ATTTTGCTGA TAAAGGCCTG AAGCACATTG AGAGAGCTTC TCTGGATGAA 
GGATTCAGAG AGCTGCTCGT CTCTACTGGA CTGAACTCCC CTGAGGCTCT GGCTGTAGAC 
TGGATTCAAC GCAAGCTCTA CTGGACTGAT AGTGGATTGT CCTCGATTTC CCGCAGCAGC 
TTAAATGGTC TTGACAGAGA GATCTTTATT AATGAAAACA TCCAGAAACC CAGAGGCATC 
GCTCTTCATC CTCAGGCACA GAAGATATAT TGGACAGATA TGGGTGATCG TCCTGCAGTG 
GAGCGGTCAG GTCTGGACAA AGACCTGCGT GAAGCAGTGG TCAGCACCGG TCTGGTGTCT 
CCCAGTGGAC TGGCTGTGGA TCATGGCTCT CAGCGTCTGT ACTGGTGTGA CATGAGCACA 
AGTGTGATTG AGTCAGCCAA CCTGGATGGT TCTCATCGCC GTGTAATAAG CCAGAACCAA 
GTAGATCGTC CATTTGATAT CGCAGTATTT GAGAACGTGC TCTGGGTCAG TGATCTGGAG 
AATCACCTGA TCTTCCGGCT GGACATGAGA TCTGATCAAA ACCCTGAGCG TCTACTTGTT 
GACTCCATTC AACCAGCTGC TCTGGTTGTT GTCCATCCTC TTGCCAAACC AGGAGCAGAT 
GTTTGTCTGG ATGGAAATGG AGGATGTGCT CAAGTGTGTG CGAGCAGACT GGGACTCCCT 
CACTGCTCCT GTCACACCAA TCAAGTTCTT TCAGCTGATG GCAAGGGATG TCGAATGATT 
AATGGATCAT TCAGTGAGTC CAGTGAAGGT GGATCAAATG ATGGTCTGAG AAACAAAACC 
CTGAATGATG AGAGCACACC ACTGGCAATG CTTTACACAG AGAAGATGGT TTCAGATCAG 
GATGACTGCT TCTCTCTCAG CTGTGTTGTG AACGCTCAGT GTTTTCTGCA GGAGGGCCGG 
GCTGTGTGTC AATGTGTGAG GGGTTTCACT GGTGATGGGG AGTTATGTGT GGACGTTGAT 
GAATGCAAAG CAGGCCTGGC AGATTGTAGT GTTTCTGAGG CTGAATGTGT GAACACAGCG 
GGCGGATATT TCTGTCAGTG CAAAAACGGA TTCAGCGGAG ATGGTCATCA CTGTGTTGAT 
ATAGATGAGT GTCGACTAGA TTTGCATGAC TGCGATGTAA ACGCTGAATG TTTAAATGCC 
GTAGGAGAAT ATCAATGTAG ATGCCGATCC GGCTTCACAG GCACTGGTTT CAGCTGTCAA 
GCAGAGTTTA ATGGCACCTC CTTGTGGCCG AGTACTGCTA GTCCTCCTGA TGTCACTTCA 
ACATTGCAAC ACAAAAATGG CGTGCAGAGC TGCCCCTCTA CACACGACTC GTATTGTCTG 
TATGATGGAG TCTGCTTCTA TTTTCCTGAG ATGGAGTCTT ACGCCTGCAA CTGTGTGCTA 
GGTTACATGG GGGAGCGCTG TCAGTTCAGT GATCTTGAAT GGTGGGAGTT GCAGCAGGCT 
GAAGAGGGGA AGAGGAGGAA TATGGTTATA GCCGTGTGTA TTGTGCTCCT CATCACCATT 
CTCTCCATCG CTGCCTGCAT CACCTTCTGC TATAGGCCTA AAAGACATTT TGGAGGGTGT 
TCCTTACAGG ACAGTGTAGG AGAAATGAGT GCGAGTGAAG ACAGTTTCAC TGAGACCACA 
ACAGCCACTC CAGAGGTCTA TGTAGTTTTG GACACTTCTA CTTGCACTGC TGATAAGGTC 
CTTCATGTAC AGTCTACCAC ATCAACAATA TGCTCCTCTT GCCCTACACA AACAGGAGAC 
AGATTTTCTT CAGAAGAGGC TGGGAAGCTA CAAAGAGATG GTTGTAGCCT GGCTGTTGCC 
ATCTGCTCCG TATCCTGCGA TATTCCCAAA ATACTGCTCA CAGAGAAAAC CACTGATAAT 
CTAATCTCCT TAGAGGATGC TCAGAGTCCG ACATCATCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991294.1
CDS	223..3567
Translation	MNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQISRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGKNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYAGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCLSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSDQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCASRLGLPHCSCHTNQVLSADGKGCRMINGSFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQAEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPEMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTGDRFSSEEAGKLQRDGCSLAVAICSVSCDIPKILLTEKTTDNLISLEDAQSPTSSQ

Target ORF information:

RefSeq Version	NM_205731.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epidermal growth factor (egf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205731.1

ORF Insert Sequence:

ATGAACCTCG ACGGAGGGGA CCGGAGAAGA ATCGTTTCTA GGGCGGGAAG GTCCATTTTG 
TTGGACTTTC ACTTAAGTGA GTGGACAATG TTTTGGGCGG ACACACAAAC AGGACAAATC 
AGCAGAGCAG GACTAGATGG AACACGAAGA CAGAAGCTTC TCTCCTCAGT GAAGGGAATC 
ACAGGCCTGG TTGTAGACTG GATTGAAAAC TCAGTCCTCT GGAGCAATGC AGATGAAGGA 
ACGATACAGA GAATGGACAC AGATGGCAAG AACGACAGAG TTGTCTTGAG AAATCTGTCT 
CAGCCCAGAG GTGTTGTTGT TGATCCAAAT GAAAGATATA TTTTCTGGTT GTCAGATGGA 
GCAACTTCCA GCATCCAGCG ATCAGACATG ACAGGTAAAA TGATGAGCAC AGTGCTGAAA 
GTAGCGGACA GACTGAAGGT TCTGGCTGTT GACCACCGGG ACAGGAGACT TTTCTGGGTT 
CAGCAAGGCC AAAGAGGACA CACTGCTATG GGCTCCTGCA ACTATGATGG AAACATCATC 
AATGTCTTTA ATCAGCAATT CAGACCTCAA TCTCTGAGAA TGACGATTTT CTTGGATTAT 
GTGTATCTGT CAGACTCAAA GTCAAAAACA ATAACTCGAT TGAACAAGTA CGCAGGGGGT 
CGACAAGAGA ATGTCAGTTC TCAAAGGATG CCACATTCCC CTGCTGATGT TAAAGTGGTG 
CATCCCATCA ATCAACCGGT GGTGGAAACT CCTTTTACAC CCGGCTGCAG TCGACACACA 
GGAGAGTGTG TGAAAGTGTG CTCCAGTCAC ACTGACACTG GTCTCTGCGG CTGCAAGGAT 
GGATTTACCC TCAGTAAACA TGGAAACATC TGTGAAGATG TCAATGAATG TTCCCTATGG 
AATCACGGCT GCTCTCTGGG ATGCGAGAAC GTTCCGGGCT CTTACTTCTG CACCTGTCCT 
GAGGGTTATC TGTTACTGCC CGATTTAAAG ACCTGCCAAG AGAACAAGCC TTGTGTGGGG 
AAAGCTGTGG ATTGTGATCA TGCATGTGTT CACACAGCAC AAGGAGATAT GTGTGTGTGT 
CCTGAAGGAT CCCTACTCAA TCCTGATGGC CAATCTTGTA CAGGCTGTTT GTCTGCAGAT 
CGTGGCGGCT GCAGTCAGAT GTGTGTGACT TTATATCCAG GCCGGTGGGT TTGTGAATGC 
CATCCTGGAT ACCAAATACA GCCTGATGGA AAACACTGCG CTGCCACTGG ACCTCCAGCG 
GACCTGTTGT TTGCCAATAT AGTGGATTTA AGGAAGATTA ACACGGATGG CAAACTGAGC 
ATAAAACTGC TGGAAAAACC AGGAGGCAAT ATTACAGCAG TGGACTATGA TCCAGTGACG 
AACAAGGTGT ATTTTGCTGA TAAAGGCCTG AAGCACATTG AGAGAGCTTC TCTGGATGAA 
GGATTCAGAG AGCTGCTCGT CTCTACTGGA CTGAACTCCC CTGAGGCTCT GGCTGTAGAC 
TGGATTCAAC GCAAGCTCTA CTGGACTGAT AGTGGATTGT CCTCGATTTC CCGCAGCAGC 
TTAAATGGTC TTGACAGAGA GATCTTTATT AATGAAAACA TCCAGAAACC CAGAGGCATC 
GCTCTTCATC CTCAGGCACA GAAGATATAT TGGACAGATA TGGGTGATCG TCCTGCAGTG 
GAGCGGTCAG GTCTGGACAA AGACCTGCGT GAAGCAGTGG TCAGCACCGG TCTGGTGTCT 
CCCAGTGGAC TGGCTGTGGA TCATGGCTCT CAGCGTCTGT ACTGGTGTGA CATGAGCACA 
AGTGTGATTG AGTCAGCCAA CCTGGATGGT TCTCATCGCC GTGTAATAAG CCAGAACCAA 
GTAGATCGTC CATTTGATAT CGCAGTATTT GAGAACGTGC TCTGGGTCAG TGATCTGGAG 
AATCACCTGA TCTTCCGGCT GGACATGAGA TCTGATCAAA ACCCTGAGCG TCTACTTGTT 
GACTCCATTC AACCAGCTGC TCTGGTTGTT GTCCATCCTC TTGCCAAACC AGGAGCAGAT 
GTTTGTCTGG ATGGAAATGG AGGATGTGCT CAAGTGTGTG CGAGCAGACT GGGACTCCCT 
CACTGCTCCT GTCACACCAA TCAAGTTCTT TCAGCTGATG GCAAGGGATG TCGAATGATT 
AATGGATCAT TCAGTGAGTC CAGTGAAGGT GGATCAAATG ATGGTCTGAG AAACAAAACC 
CTGAATGATG AGAGCACACC ACTGGCAATG CTTTACACAG AGAAGATGGT TTCAGATCAG 
GATGACTGCT TCTCTCTCAG CTGTGTTGTG AACGCTCAGT GTTTTCTGCA GGAGGGCCGG 
GCTGTGTGTC AATGTGTGAG GGGTTTCACT GGTGATGGGG AGTTATGTGT GGACGTTGAT 
GAATGCAAAG CAGGCCTGGC AGATTGTAGT GTTTCTGAGG CTGAATGTGT GAACACAGCG 
GGCGGATATT TCTGTCAGTG CAAAAACGGA TTCAGCGGAG ATGGTCATCA CTGTGTTGAT 
ATAGATGAGT GTCGACTAGA TTTGCATGAC TGCGATGTAA ACGCTGAATG TTTAAATGCC 
GTAGGAGAAT ATCAATGTAG ATGCCGATCC GGCTTCACAG GCACTGGTTT CAGCTGTCAA 
GCAGAGTTTA ATGGCACCTC CTTGTGGCCG AGTACTGCTA GTCCTCCTGA TGTCACTTCA 
ACATTGCAAC ACAAAAATGG CGTGCAGAGC TGCCCCTCTA CACACGACTC GTATTGTCTG 
TATGATGGAG TCTGCTTCTA TTTTCCTGAG ATGGAGTCTT ACGCCTGCAA CTGTGTGCTA 
GGTTACATGG GGGAGCGCTG TCAGTTCAGT GATCTTGAAT GGTGGGAGTT GCAGCAGGCT 
GAAGAGGGGA AGAGGAGGAA TATGGTTATA GCCGTGTGTA TTGTGCTCCT CATCACCATT 
CTCTCCATCG CTGCCTGCAT CACCTTCTGC TATAGGCCTA AAAGACATTT TGGAGGGTGT 
TCCTTACAGG ACAGTGTAGG AGAAATGAGT GCGAGTGAAG ACAGTTTCAC TGAGACCACA 
ACAGCCACTC CAGAGGTCTA TGTAGTTTTG GACACTTCTA CTTGCACTGC TGATAAGGTC 
CTTCATGTAC AGTCTACCAC ATCAACAATA TGCTCCTCTT GCCCTACACA AACAGGAGAC 
AGATTTTCTT CAGAAGAGGC TGGGAAGCTA CAAAGAGATG GTTGTAGCCT GGCTGTTGCC 
ATCTGCTCCG TATCCTGCGA TATTCCCAAA ATACTGCTCA CAGAGAAAAC CACTGATAAT 
CTAATCTCCT TAGAGGATGC TCAGAGTCCG ACATCATCCC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( NM_001365527.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352456.1 Danio rerio epidermal growth factor (egf), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa19813
Clone ID Related Accession (Same CDS sequence)	XM_005157314.3 , XM_005157314.4 , NM_001365527.1
Accession Version	NM_001365527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Danio rerio(zebrafish)
Product	pro-epidermal growth factor isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510944.11. On or before Aug 30, 2018 this sequence version replaced NM_205731.1, XM_005157314.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GFIL01009301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGGAGACA GATTTTCTTC AGAAGAGGCT GGGAAGCTAC AAAGAGATGG TTGTAGCCTG 
GCTGTTGCCA TCTGCTCCGT ATCCTGCGAT ATTCCCAAAA TACTGCTCAC AGAGAAAACC 
ACTGATAATC TAATCTCCTT AGAGGATGCT CAGAGTCCGA CATCATCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352456.1
CDS	627..4160
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSGQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCVSRLGLPHCSCHTNHVLSADGKGCRMINASFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQAEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPDMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTGDRFSSEEAGKLQRDGCSLAVAICSVSCDIPKILLTEKTTDNLISLEDAQSPTSSQ

Target ORF information:

RefSeq Version	NM_001365527.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365527.1

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGGAGACA GATTTTCTTC AGAAGAGGCT GGGAAGCTAC AAAGAGATGG TTGTAGCCTG 
GCTGTTGCCA TCTGCTCCGT ATCCTGCGAT ATTCCCAAAA TACTGCTCAC AGAGAAAACC 
ACTGATAATC TAATCTCCTT AGAGGATGCT CAGAGTCCGA CATCATCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( NM_001365528.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352457.1 Danio rerio epidermal growth factor (egf), transcript variant 2, mRNA.

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CloneID	ODa40761
Clone ID Related Accession (Same CDS sequence)	XM_021480997.1 , NM_001365528.1
Accession Version	NM_001365528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Danio rerio(zebrafish)
Product	pro-epidermal growth factor isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510944.11. On Aug 30, 2018 this sequence version replaced XM_021480997.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG AGTTTAATGG CACCTCCTTG TGGCCGAGTA CTGCTAGTCC TCCTGATGTC 
ACTTCAACAT TGCAACACAA AAATGGCGTG CAGAGCTGCC CCTCTACACA TGACTCGTAT 
TGTCTGTATG ATGGAGTCTG CTTCTATTTT CCTGACATGG AGTCTTACGC CTGCAACTGT 
GTGCTAGGTT ACATGGGGGA GCGCTGTCAG TTCAGTGATC TTGAATGGTG GGAGTTGCAG 
CAGGCTGAAG AGGGGAAGAG GAGGAATATG GTTATAGCCG TGTGTATTGT GCTCCTCATC 
ACCATTCTCT CCATCGCTGC CTGCATCACC TTCTGCTATA GGCCTAAAAG ACATTTTGGA 
GGGTGTTCCT TACAGGACAG TGTAGGAGAA ATGAGTGCGA GTGAAGACAG TTTCACTGAG 
ACCACAACAG CCACTCCAGA GGTCTATGTA GTTTTGGACA CTTCTACTTG CACTGCTGAT 
AAGGTCCTTC ATGTACAGTC TACCACATCA ACAATATGCT CCTCTTGCCC TACACAAACA 
GGAGACAGAT TTTCTTCAGA AGAGGCTGGG AAGCTACAAA GAGATGGTTG TAGCCTGGCT 
GTTGCCATCT GCTCCGTATC CTGCGATATT CCCAAAATAC TGCTCACAGA GAAAACCACT 
GATAATCTAA TCTCCTTAGA GGATGCTCAG AGTCCGACAT CATCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352457.1
CDS	627..4157
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSGQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCVSRLGLPHCSCHTNHVLSADGKGCRMINASFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPDMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTGDRFSSEEAGKLQRDGCSLAVAICSVSCDIPKILLTEKTTDNLISLEDAQSPTSSQ

Target ORF information:

RefSeq Version	NM_001365528.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365528.1

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG AGTTTAATGG CACCTCCTTG TGGCCGAGTA CTGCTAGTCC TCCTGATGTC 
ACTTCAACAT TGCAACACAA AAATGGCGTG CAGAGCTGCC CCTCTACACA TGACTCGTAT 
TGTCTGTATG ATGGAGTCTG CTTCTATTTT CCTGACATGG AGTCTTACGC CTGCAACTGT 
GTGCTAGGTT ACATGGGGGA GCGCTGTCAG TTCAGTGATC TTGAATGGTG GGAGTTGCAG 
CAGGCTGAAG AGGGGAAGAG GAGGAATATG GTTATAGCCG TGTGTATTGT GCTCCTCATC 
ACCATTCTCT CCATCGCTGC CTGCATCACC TTCTGCTATA GGCCTAAAAG ACATTTTGGA 
GGGTGTTCCT TACAGGACAG TGTAGGAGAA ATGAGTGCGA GTGAAGACAG TTTCACTGAG 
ACCACAACAG CCACTCCAGA GGTCTATGTA GTTTTGGACA CTTCTACTTG CACTGCTGAT 
AAGGTCCTTC ATGTACAGTC TACCACATCA ACAATATGCT CCTCTTGCCC TACACAAACA 
GGAGACAGAT TTTCTTCAGA AGAGGCTGGG AAGCTACAAA GAGATGGTTG TAGCCTGGCT 
GTTGCCATCT GCTCCGTATC CTGCGATATT CCCAAAATAC TGCTCACAGA GAAAACCACT 
GATAATCTAA TCTCCTTAGA GGATGCTCAG AGTCCGACAT CATCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( XM_021480998.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336673.1 Danio rerio epidermal growth factor (egf), transcript variant X3, mRNA.

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CloneID	ODa148729
Clone ID Related Accession (Same CDS sequence)	XM_021480998.1
Accession Version	XM_021480998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pro-epidermal growth factor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGTTTTGA GGACACTGAA AGACGATGTT GTTCATGATG AAATAACATC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336673.1
CDS	622..4038
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSGQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCVSRLGLPHCSCHTNHVLSADGKGCRMINASFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQAEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPDMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTVLRTLKDDVVHDEITSD

Target ORF information:

RefSeq Version	XM_021480998.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480998.1

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGTTTTGA GGACACTGAA AGACGATGTT GTTCATGATG AAATAACATC TGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( XM_005157314.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157371.1 Danio rerio epidermal growth factor (egf), transcript variant X2, mRNA.

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CloneID	ODa19813
Clone ID Related Accession (Same CDS sequence)	XM_005157314.3 , XM_005157314.4 , NM_001365527.1
Accession Version	XM_005157314.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pro-epidermal growth factor isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157314.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGGAGACA GATTTTCTTC AGAAGAGGCT GGGAAGCTAC AAAGAGATGG TTGTAGCCTG 
GCTGTTGCCA TCTGCTCCGT ATCCTGCGAT ATTCCCAAAA TACTGCTCAC AGAGAAAACC 
ACTGATAATC TAATCTCCTT AGAGGATGCT CAGAGTCCGA CATCATCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157371.1
CDS	373..3906
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSGQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCVSRLGLPHCSCHTNHVLSADGKGCRMINASFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQAEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPDMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTGDRFSSEEAGKLQRDGCSLAVAICSVSCDIPKILLTEKTTDNLISLEDAQSPTSSQ

Target ORF information:

RefSeq Version	XM_005157314.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157314.3

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGGAGACA GATTTTCTTC AGAAGAGGCT GGGAAGCTAC AAAGAGATGG TTGTAGCCTG 
GCTGTTGCCA TCTGCTCCGT ATCCTGCGAT ATTCCCAAAA TACTGCTCAC AGAGAAAACC 
ACTGATAATC TAATCTCCTT AGAGGATGCT CAGAGTCCGA CATCATCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( XM_005157314.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157371.1 Danio rerio epidermal growth factor (egf), transcript variant X1, mRNA.

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CloneID	ODa19813
Clone ID Related Accession (Same CDS sequence)	XM_005157314.3 , XM_005157314.4 , NM_001365527.1
Accession Version	XM_005157314.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pro-epidermal growth factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157314.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGGAGACA GATTTTCTTC AGAAGAGGCT GGGAAGCTAC AAAGAGATGG TTGTAGCCTG 
GCTGTTGCCA TCTGCTCCGT ATCCTGCGAT ATTCCCAAAA TACTGCTCAC AGAGAAAACC 
ACTGATAATC TAATCTCCTT AGAGGATGCT CAGAGTCCGA CATCATCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157371.1
CDS	619..4152
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSGQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCVSRLGLPHCSCHTNHVLSADGKGCRMINASFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQAEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPDMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTGDRFSSEEAGKLQRDGCSLAVAICSVSCDIPKILLTEKTTDNLISLEDAQSPTSSQ

Target ORF information:

RefSeq Version	XM_005157314.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157314.4

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG CAGAGTTTAA TGGCACCTCC TTGTGGCCGA GTACTGCTAG TCCTCCTGAT 
GTCACTTCAA CATTGCAACA CAAAAATGGC GTGCAGAGCT GCCCCTCTAC ACATGACTCG 
TATTGTCTGT ATGATGGAGT CTGCTTCTAT TTTCCTGACA TGGAGTCTTA CGCCTGCAAC 
TGTGTGCTAG GTTACATGGG GGAGCGCTGT CAGTTCAGTG ATCTTGAATG GTGGGAGTTG 
CAGCAGGCTG AAGAGGGGAA GAGGAGGAAT ATGGTTATAG CCGTGTGTAT TGTGCTCCTC 
ATCACCATTC TCTCCATCGC TGCCTGCATC ACCTTCTGCT ATAGGCCTAA AAGACATTTT 
GGAGGGTGTT CCTTACAGGA CAGTGTAGGA GAAATGAGTG CGAGTGAAGA CAGTTTCACT 
GAGACCACAA CAGCCACTCC AGAGGTCTAT GTAGTTTTGG ACACTTCTAC TTGCACTGCT 
GATAAGGTCC TTCATGTACA GTCTACCACA TCAACAATAT GCTCCTCTTG CCCTACACAA 
ACAGGAGACA GATTTTCTTC AGAAGAGGCT GGGAAGCTAC AAAGAGATGG TTGTAGCCTG 
GCTGTTGCCA TCTGCTCCGT ATCCTGCGAT ATTCCCAAAA TACTGCTCAC AGAGAAAACC 
ACTGATAATC TAATCTCCTT AGAGGATGCT CAGAGTCCGA CATCATCCCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( XM_021480997.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336672.1 Danio rerio epidermal growth factor (egf), transcript variant X2, mRNA.

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CloneID	ODa40761
Clone ID Related Accession (Same CDS sequence)	XM_021480997.1 , NM_001365528.1
Accession Version	XM_021480997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pro-epidermal growth factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG AGTTTAATGG CACCTCCTTG TGGCCGAGTA CTGCTAGTCC TCCTGATGTC 
ACTTCAACAT TGCAACACAA AAATGGCGTG CAGAGCTGCC CCTCTACACA TGACTCGTAT 
TGTCTGTATG ATGGAGTCTG CTTCTATTTT CCTGACATGG AGTCTTACGC CTGCAACTGT 
GTGCTAGGTT ACATGGGGGA GCGCTGTCAG TTCAGTGATC TTGAATGGTG GGAGTTGCAG 
CAGGCTGAAG AGGGGAAGAG GAGGAATATG GTTATAGCCG TGTGTATTGT GCTCCTCATC 
ACCATTCTCT CCATCGCTGC CTGCATCACC TTCTGCTATA GGCCTAAAAG ACATTTTGGA 
GGGTGTTCCT TACAGGACAG TGTAGGAGAA ATGAGTGCGA GTGAAGACAG TTTCACTGAG 
ACCACAACAG CCACTCCAGA GGTCTATGTA GTTTTGGACA CTTCTACTTG CACTGCTGAT 
AAGGTCCTTC ATGTACAGTC TACCACATCA ACAATATGCT CCTCTTGCCC TACACAAACA 
GGAGACAGAT TTTCTTCAGA AGAGGCTGGG AAGCTACAAA GAGATGGTTG TAGCCTGGCT 
GTTGCCATCT GCTCCGTATC CTGCGATATT CCCAAAATAC TGCTCACAGA GAAAACCACT 
GATAATCTAA TCTCCTTAGA GGATGCTCAG AGTCCGACAT CATCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336672.1
CDS	620..4150
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQVDRPFDIAVFENVLWVSDLENHLIFRLDMRSGQNPERLLVDSIQPAALVVVHPLAKPGADVCLDGNGGCAQVCVSRLGLPHCSCHTNHVLSADGKGCRMINASFSESSEGGSNDGLRNKTLNDESTPLAMLYTEKMVSDQDDCFSLSCVVNAQCFLQEGRAVCQCVRGFTGDGELCVDVDECKAGLADCSVSEAECVNTAGGYFCQCKNGFSGDGHHCVDIDECRLDLHDCDVNAECLNAVGEYQCRCRSGFTGTGFSCQEFNGTSLWPSTASPPDVTSTLQHKNGVQSCPSTHDSYCLYDGVCFYFPDMESYACNCVLGYMGERCQFSDLEWWELQQAEEGKRRNMVIAVCIVLLITILSIAACITFCYRPKRHFGGCSLQDSVGEMSASEDSFTETTTATPEVYVVLDTSTCTADKVLHVQSTTSTICSSCPTQTGDRFSSEEAGKLQRDGCSLAVAICSVSCDIPKILLTEKTTDNLISLEDAQSPTSSQ

Target ORF information:

RefSeq Version	XM_021480997.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480997.1

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAG TAGATCGTCC ATTTGATATC GCAGTATTTG AGAACGTGCT CTGGGTCAGT 
GATCTGGAGA ATCACCTGAT CTTCCGGCTG GACATGAGAT CTGGTCAAAA CCCTGAGCGT 
CTACTTGTTG ACTCCATTCA ACCAGCTGCT CTGGTTGTTG TCCATCCTCT TGCCAAACCA 
GGAGCAGATG TTTGTCTGGA TGGAAATGGA GGATGTGCTC AAGTGTGTGT GAGCAGACTG 
GGACTCCCTC ACTGCTCCTG TCACACCAAT CACGTTCTTT CAGCTGATGG CAAAGGGTGT 
CGGATGATTA ATGCATCATT CAGTGAGTCC AGTGAAGGTG GATCAAATGA TGGTCTAAGA 
AACAAAACCC TGAATGATGA GAGCACACCA CTGGCAATGC TTTACACAGA GAAGATGGTT 
TCAGATCAGG ATGACTGCTT CTCTCTCAGC TGTGTTGTGA ACGCTCAGTG TTTTCTGCAG 
GAGGGCCGGG CTGTGTGTCA ATGTGTGAGG GGTTTCACTG GTGATGGGGA GTTATGTGTG 
GACGTTGATG AATGCAAAGC AGGCCTGGCA GATTGTAGTG TTTCTGAGGC TGAATGTGTG 
AACACAGCGG GCGGATATTT CTGTCAGTGC AAAAATGGAT TCAGCGGAGA TGGTCATCAC 
TGTGTTGATA TAGATGAGTG TCGACTAGAT TTGCATGACT GCGATGTAAA CGCTGAATGT 
TTAAATGCCG TAGGAGAATA TCAATGTAGA TGCCGATCTG GCTTCACAGG CACTGGTTTC 
AGCTGTCAAG AGTTTAATGG CACCTCCTTG TGGCCGAGTA CTGCTAGTCC TCCTGATGTC 
ACTTCAACAT TGCAACACAA AAATGGCGTG CAGAGCTGCC CCTCTACACA TGACTCGTAT 
TGTCTGTATG ATGGAGTCTG CTTCTATTTT CCTGACATGG AGTCTTACGC CTGCAACTGT 
GTGCTAGGTT ACATGGGGGA GCGCTGTCAG TTCAGTGATC TTGAATGGTG GGAGTTGCAG 
CAGGCTGAAG AGGGGAAGAG GAGGAATATG GTTATAGCCG TGTGTATTGT GCTCCTCATC 
ACCATTCTCT CCATCGCTGC CTGCATCACC TTCTGCTATA GGCCTAAAAG ACATTTTGGA 
GGGTGTTCCT TACAGGACAG TGTAGGAGAA ATGAGTGCGA GTGAAGACAG TTTCACTGAG 
ACCACAACAG CCACTCCAGA GGTCTATGTA GTTTTGGACA CTTCTACTTG CACTGCTGAT 
AAGGTCCTTC ATGTACAGTC TACCACATCA ACAATATGCT CCTCTTGCCC TACACAAACA 
GGAGACAGAT TTTCTTCAGA AGAGGCTGGG AAGCTACAAA GAGATGGTTG TAGCCTGGCT 
GTTGCCATCT GCTCCGTATC CTGCGATATT CCCAAAATAC TGCTCACAGA GAAAACCACT 
GATAATCTAA TCTCCTTAGA GGATGCTCAG AGTCCGACAT CATCCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

egf ( XM_017359001.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214490.1 Danio rerio epidermal growth factor (egf), transcript variant X1, mRNA.

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CloneID	ODa54902
Clone ID Related Accession (Same CDS sequence)	XM_017359001.1
Accession Version	XM_017359001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	pro-epidermal growth factor isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAA TCGTCCATTT GATATCGCAG TATTTGAGAA CGTGCTCTGG GTCAGTGATC 
TGGAGAATCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214490.1
CDS	373..2487
Translation	MCWRTIALTFGLWSWMVSMDPLRASGPAEEALCWGGRAWAAGNFNCVEPEPYLIVGLGNSIQRMNLDGGDRRRIVSRAGRSILLDFHLSEWTMFWADTQTGQIRRAGLDGTRRQKLLSSVKGITGLVVDWIENSVLWSNADEGTIQRMDTDGRNDRVVLRNLSQPRGVVVDPNERYIFWLSDGATSSIQRSDMTGKMMSTVLKVADRLKVLAVDHRDRRLFWVQQGQRGHTAMGSCNYDGNIINVFNQQFRPQSLRMTIFLDYVYLSDSKSKTITRLNKYTGGRQENVSSQRMPHSPADVKVVHPINQPVVETPFTPGCSRHTGECVKVCSSHTDTGLCGCKDGFTLSKHGNICEDVNECSLWNHGCSLGCENVPGSYFCTCPEGYLLLPDLKTCQENKPCVGKAVDCDHACVHTAQGDMCVCPEGSLLNPDGQSCTGCFSADRGGCSQMCVTLYPGRWVCECHPGYQIQPDGKHCAATGPPADLLFANIVDLRKINTDGKLSIKLLEKPGGNITAVDYDPVTNKVYFADKGLKHIERASLDEGFRELLVSTGLNSPEALAVDWIQRKLYWTDSGLSSISRSSLNGLDREIFINENIQKPRGIALHPQAQKIYWTDMGDRPAVERSGLDKDLREAVVSTGLVSPSGLAVDHGSQRLYWCDMSTSVIESANLDGSHRRVISQNQIVHLISQYLRTCSGSVIWRIT

Target ORF information:

RefSeq Version	XM_017359001.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio epidermal growth factor (egf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017359001.1

ORF Insert Sequence:

ATGTGCTGGA GGACGATAGC GCTCACTTTT GGATTGTGGT CGTGGATGGT GAGTATGGAC 
CCCCTACGAG CTTCAGGCCC TGCCGAGGAG GCTTTATGTT GGGGTGGACG AGCCTGGGCT 
GCTGGAAACT TCAACTGTGT GGAACCTGAG CCATATCTAA TAGTCGGCCT CGGAAATAGC 
ATCCAGAGAA TGAACCTCGA TGGAGGGGAC CGGAGAAGAA TCGTTTCCAG GGCGGGAAGG 
TCCATTTTGT TGGACTTTCA CTTAAGTGAG TGGACGATGT TTTGGGCGGA CACACAAACA 
GGACAAATCA GAAGAGCAGG ACTAGATGGA ACACGGAGAC AGAAGCTTCT CTCCTCAGTG 
AAGGGAATCA CGGGTCTGGT TGTGGACTGG ATTGAAAACT CAGTCCTCTG GAGCAATGCA 
GATGAAGGAA CAATACAGAG AATGGACACT GATGGCAGGA ACGACAGAGT TGTCTTGAGA 
AATCTGTCTC AGCCTAGAGG TGTTGTTGTT GATCCAAATG AAAGATATAT TTTCTGGTTG 
TCAGATGGAG CGACTTCCAG CATCCAGCGA TCAGACATGA CAGGTAAAAT GATGAGCACA 
GTGCTGAAAG TAGCGGACAG ACTGAAGGTT CTGGCTGTTG ACCACCGGGA CAGGAGACTT 
TTCTGGGTTC AGCAAGGCCA AAGAGGACAC ACTGCTATGG GCTCCTGCAA CTATGATGGA 
AACATTATCA ATGTCTTTAA TCAGCAATTC AGACCTCAAT CTCTGAGAAT GACGATTTTC 
TTGGATTATG TGTATCTGTC AGACTCAAAG TCAAAAACAA TAACTCGATT GAACAAGTAC 
ACAGGGGGTC GACAAGAGAA TGTCAGTTCT CAAAGGATGC CACATTCCCC TGCTGATGTT 
AAAGTGGTGC ATCCCATCAA TCAACCGGTG GTGGAAACTC CTTTTACACC CGGCTGCAGT 
CGACACACAG GAGAGTGTGT GAAAGTGTGC TCCAGTCACA CTGACACTGG TCTCTGCGGC 
TGCAAGGATG GATTTACCCT CAGTAAACAT GGAAACATCT GTGAAGATGT CAATGAATGT 
TCCCTATGGA ATCACGGCTG CTCTCTGGGA TGCGAGAACG TTCCGGGCTC TTACTTCTGC 
ACCTGTCCTG AGGGTTACCT GTTACTGCCC GATTTAAAGA CCTGCCAAGA GAACAAGCCT 
TGTGTGGGGA AAGCTGTGGA TTGTGATCAT GCATGTGTTC ACACAGCACA AGGAGATATG 
TGTGTGTGTC CTGAAGGATC CCTACTCAAT CCTGATGGCC AATCTTGTAC AGGCTGTTTT 
TCTGCAGATC GTGGCGGCTG CAGTCAGATG TGTGTGACTT TATATCCAGG CCGGTGGGTT 
TGTGAATGCC ATCCTGGATA CCAAATACAG CCTGATGGAA AACACTGCGC TGCCACTGGA 
CCTCCAGCGG ACCTGTTGTT TGCCAATATA GTGGATTTAA GGAAGATTAA CACGGATGGC 
AAACTGAGCA TAAAACTGCT GGAAAAACCA GGAGGCAATA TTACAGCAGT GGACTATGAT 
CCAGTGACGA ACAAGGTGTA TTTTGCTGAT AAAGGCCTGA AGCACATTGA GAGAGCTTCT 
CTGGATGAAG GATTCAGAGA GCTGCTCGTC TCTACTGGAC TGAACTCCCC TGAGGCTCTG 
GCTGTAGACT GGATTCAACG CAAGCTCTAC TGGACTGATA GTGGATTGTC CTCGATTTCC 
CGCAGCAGCT TAAATGGTCT TGACAGAGAG ATCTTTATTA ATGAAAACAT CCAGAAACCC 
AGAGGCATCG CTCTTCATCC TCAGGCACAG AAGATATATT GGACAGATAT GGGTGATCGT 
CCTGCAGTGG AGCGGTCAGG TCTGGACAAA GACCTGCGTG AAGCAGTGGT CAGCACCGGT 
CTGGTGTCTC CCAGTGGACT GGCTGTGGAT CATGGCTCTC AGCGTCTGTA CTGGTGTGAC 
ATGAGCACAA GTGTGATTGA GTCAGCCAAC CTGGATGGTT CTCATCGCCG TGTAATAAGC 
CAGAACCAAA TCGTCCATTT GATATCGCAG TATTTGAGAA CGTGCTCTGG GTCAGTGATC 
TGGAGAATCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypersensitive assessment of aryl hydrocarbon receptor transcriptional activity using a novel truncated
FASEB journal : official publication of the Federation of American Societies for Experimental Biology(2018 Jan)
Luo JJ,Su DS,Xie SL,Liu Y,Liu P,Yang XJ,Pei DS

Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative Programs.
Developmental cell43(6)659-672.e5(2017 Dec)
Hui SP,Sheng DZ,Sugimoto K,Gonzalez-Rajal A,Nakagawa S,Hesselson D,Kikuchi K

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egf cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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