dock8 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dock8
Entrez Gene ID	403040
Full Name	dedicator of cytokinesis 8
Synonyms	im:7154406,si:dkey-91m11.1
General protein information	Preferred Names dedicator of cytokinesis 8 Names dedicator of cytokinesis protein 8
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001920072.6	XP_001920107.4	dedicator of cytokinesis protein 8 isoform X1
XM_009304069.2	XP_009302344.1	dedicator of cytokinesis protein 8 isoform X2
XM_009304068.2	XP_009302343.1	dedicator of cytokinesis protein 8 isoform X2
XM_001920072.7	XP_001920107.4	dedicator of cytokinesis protein 8 isoform X1
XM_021478460.1	XP_021334135.1	dedicator of cytokinesis protein 8 isoform X2
XM_009304069.3	XP_009302344.1	dedicator of cytokinesis protein 8 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DOCK8	NP_001178461.1
Danio rerio (zebrafish)	dock8	XP_001920107.4
Homo sapiens (human)	DOCK8	NP_982272.2
Pan troglodytes (chimpanzee)	DOCK8	XP_003312029.1
Xenopus tropicalis (tropical clawed frog)	dock8	XP_002935647.2
Canis lupus familiaris (dog)	DOCK8	XP_005615946.1
Gallus gallus (chicken)	DOCK8	XP_424926.4
	LOC698081	XP_002808151.1
Rattus norvegicus (Norway rat)	Dock8	NP_001032882.2
Mus musculus (house mouse)	Dock8	NP_083061.2

Gene Ontology

The following dock8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dock8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa14388	XM_001920072.7 Latest version!	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40759	XM_009304068.2 Latest version!	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40759	XM_009304069.3 Latest version!	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40759	XM_021478460.1 Latest version!	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa14388	XM_001920072.6	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa40759	XM_009304069.2	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dock8 ( XM_001920072.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001920107.4 Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

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CloneID	ODa14388
Clone ID Related Accession (Same CDS sequence)	XM_001920072.6 , XM_001920072.7
Accession Version	XM_001920072.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001920072.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC TGCCGGCGTC AGAGCGGCGG GCGTTCGCGC TCAAAATCAA CAGACACTCA 
TCGGCAGAGA TCCGGAGACA CTTCAGTGGG GTCAGCCATG GTTCCCTGCC GCACCACCGG 
CACAGTTACA ACGCTTCTGG CTCTTTCTGC TTCAACGTAC CTCAATCATA TGAAGCGGTT 
GAGCCAGTGG ACCTGGATGA GTTCCTGATG TCTCAGCTGC GCAGTGGCGA TGCCGAGGTC 
ATGCAGGAGT TGGGTGAGTT TCCTGATGAC GACCTGGATG TTGAGCTTGT GGAGAGGGAA 
TGCAGGACCA TCCGCCCCAC TATTGCTGAG GAAGGAGTGG AGCTGGATTC CCATGTGAGG 
GATTGTGTTC AGAGTTACAC GCAGCCTTGG CTGGTGGTGA GCAGAAGAAG TCAGGGTTAT 
GGATGGATTT CATACTCTGA TAGAACAGAC TCTAACAAAA TCTTACAGAG GCAGACATTT 
GAGTCTGACG TTCAGCCAGA CAAACAGGAG CAACTAGATA AGTCGCCGTC GCTGACAGCA 
CTGTGTGATG ATTCAGCTCG GACCACCCTC ACAAGCTCAG ATTTTAACCT GAGGGCCCTT 
CAGCCAGACC AGCGAGTGGA AAGTGTCCTG CGTTTCAGCA GCCCAGAGGA ATTGGACCGC 
TTCAACCAGG AGTCCAGACA GAGCAACCGC TATGGCGAAC TCTTTGGCCT CTACCCGCCT 
ATTGATGAGG AGGATGCTGT TGCAATTCGG CCTATTCCTG ACTGTCCAAA GGAGCATTTT 
GGCCAAAGGA TATTAGTGCG ATGCCAGACC GTCAAATTTG AGATTGAAAT CGAGCCTCTC 
TTTGCAACAA TGGCCCTCTA TGACCTGAAG GAGAAGAAGA AGATTTCAGA GAACTTCAAC 
TTTGATCTGA ACTCAGATCA GATGAAGGGT TTCCTGCGGA CCCACACTCC TCAAGTTGAC 
ACTTCCACTT TGTCCAGATC TGCAATTTTT TCCATCTCCT ACCCCTCTCC AGACATCTAT 
CTGGTCATTA AGATTGAGAA GGTTCTTCAG CAGGGAGAGA TTGGGGATTG TGCTGAACCA 
TACATGGTAC TGAAGGAGAG TGACACGACT AAGAATAGAG ACAAGCTGGA GAAGTTGCGG 
AATCAGGCGG AGGGGTTTTG TCAAAGACTG GGTCGCTACA GGATGCCATT AGCCTTTGCT 
ACGGTCAACA TCATGAGTGT CATCAACTCC ACACTGGAAC GAGAGACAAG TGACACAGAC 
AGTATTAATG GCAGGGGGAG TATGGACAAG AAGGGATTGC CGAGACGTAA CTCTGACAGA 
TTCAGCTTGA TGGAGGACAA CTACATCCTG TCAGGCTTCA AACCAGCTGT CACTATGTGC 
ACATTCATCA AACAGGAAGG AGAACGTCTG AGTGACGAAG ATCTGTTTAA GTTCCTGGCG 
GATATAAAGA GGTCATCCAG TCAGAGACGT GTTAAAATCA TACCTGGTTA TCTGAAACTG 
GAAGTGCTTC CAGCGCCGGA CACGGTTCCT GGGTGTTTGT CTCCTGAACT GATTCCAGTG 
AAGCCTGTGT CTGAGAAAAA CCAACGTCCG ATTAAAGAAG TGCTCGAGTT CCCTTCGAAG 
GAAGTTTATG TGCCTCACAA TATATACCGG AATCTGCTGT ATGTATATCC GCTGAGGTTA 
AATCTTACTA ACCGTCTGAC TTCAGCCAGA AACATTACAG TAAAAATCCA GTTCATGAGC 
GGTGAGGACT CAAGTTGTGC CATGCCTGTA ATCTTTGGAA AATCAAGTGG CCCGGAGTTC 
CTTACGGAGG TCTACACTCC AGTCACTTAC CACAACAGGT CTCCTGATTT CTATGACGAG 
ATAAAGATCC TCCTGCCAGC CCGTTTAACA GATAAACACC ACTTGCTGTT CACCTTCTTC 
CATATCAGCT GCCAGCAGAA ACAAAACCAG ACTGGAAGTG TGGAGGCTCT CATTGGATAT 
TCATGGTTAC CCATGCTGAA CAATGACCGG CTGCAGACCA GCCAGCAGTG TCTGCCAATC 
GTTCTGGACA AGCTTCCTAT CAACTATTCC CTACATACCC CAGAGAAACT GCCAGCTCAG 
GTACCTCCAG TGAAGTGGCT GGAGAATCAT AAAGGACTGT TTAACCTTGA GCTACAAGCT 
GTGTCCTCAG TTCAAACTCA GGACAGTCAC CTGGAGCGTT TCTTCACATT ATGTCATGCT 
TTGGAGGGCA AATCCACATT CCCCATCCGA GTGCGAGATG AGAAGATTCA GGAAAACATG 
TTTGAGCATG AGCTGAAGCT CAGCATCATT TCACTGTCCT CCGCCGGCCT GGAGCCGCTG 
GTGCTTTTTC TCCATCAGGT CCTCGACAAG CTCTTCCGTC TCATCATGCA GCCCATGGTC 
ATCGCTGGAC AGACTGCAAA TCTGGCCCAA ATTGCGTTTG AGTCAGTGGT GTCGGTCATC 
AACAGTCTTC ACAACAGTCA GGAGCTGGCA AAAGACCATC AGGGCAGAAA CTGTCTCCTC 
GCAACATACC TGTACTTTGT GTTTCGCTTG CCAGATACTC AATATGAAAT CCACACCTCA 
GGTACAGGAG GTCTGATGGC TCATCCAGAG GGTCGATACA GCACTTTAAC TCGCACAACG 
GCCACCACTG TTGGCTTCAT GCTGCTGCAG TCACGAATCC GTTCCAGCAG CAACCCTGAT 
ATACCAGCAC CACTGAGCCC AGAGGACACA GAGGTCAATA ACATTCTAGG AAAGGTTTTA 
AATCATCCAG GCAGTCGCAT GTCAACAGCT GCAAATGCCT CCAATTCTCC GCAGAGCTCC 
ACGGGAAGCA CGCGGCCCAC CAACAAAAAG TTGTTCCACG AGGAGCTGGC TCTTCAGATG 
GTGGTCAGCA CCGGAGTCTG CAGAGAAAAT GTCTACAAAT ACGCCTGGTT CTTCTTTGAA 
GTTCTGGTGA AGAGCATGAG TCAGCACGTG TCTCAGCTGG ATAAGAAGAC GGTCTCTCGC 
AGGAATCGAT TTTCCGACCG TTTCAAAGAT GACATCACCA CCATTGTCAA CGTGGTGACC 
GCTGAGATCG GGAACATTTT AGTAAAGCAG CAAAAGGAGC TGGAGCAGGC AGAGAAGGTT 
AACATCAGCC TGGCTTTCTT CCTGTATGAC CTGATGTCTC TGATGGATCG AGGCTTTGTG 
TTTCAGCTGG TGAAGAATTA CTGCAACCAG ATGGCAGCAA AGAGTGTGAA CATGGCGACT 
TTGATAAGTA TGAGGCTGGA GTTTTTGAGG GTCTTGTGCA GTCATGAGCA CTACTTGAAC 
CTCAGTCTCT TCTTCAGCAG CCCTTCATCT GCCCCTGCCT CCCCATCACC CTCGGTGTCC 
TCACAGACGT CCAGCTCGTG CAGTTTTCAG GACAACAAGA TCGCAGCCAT GTTTGATTTG 
TCGCAGGACT TTAAGCAGCG CCATTACTTG ACCGGACTCT TGCTAACTGA ACTCAGCACT 
GCACTGGACA TAGAGTCCGA GGGGGGCAGA GTTCAACAAA AAGCCATCAA TGCCACATAC 
AGTCTATTAT GTGCTCATGA TCTGGACCAG CGCTGCTCAA GGTCAGAGGT CAAGGCGAAG 
ATTGCTGCTC TCTACCTCCC GCTAGTGGGC ATCATTATTG ACTCCATCAA TTACCTGGAC 
TTCACAGTTT CTGACTCTCG TAGTGGTAAA GGCAAGTCTG GTGGGCCTGA GGAAGACCCT 
GACAACATTG CCACCATCAA TCAGTCTGTT GCAATGGCAA TCGCTGGAAA TCCTTTCAAC 
ACCTTAACCA GGAACGCACT GGTGTCTATG GCCTCTGTGA GTGTGAAAAC CACCAACATG 
TTGACGGCGG AGACCAGTCG CAATCTGCTG ATGTGTTTCC TGTGGATCAT CAAGAACGCA 
GACCAGAATC TGATCCAGCG CTGGACTGTG GACATGCCTT CATCTCAGCT GAGTCGACTG 
CTGGAGCTCC TCGCCATCTG CACCTCCTGC TTCGAGTACA GGGGGAAGCA GAGCTCTGAT 
AAGGTGAGCA CTCAGGCACT CCAGAAGTCT CAGCAGGCCA AGGCGCGTCT GGAGGAGGCT 
CTGCTGGGAG GAATCGGAGC CAGAGGAGAA ATGATGAAAC GGGTGGGAGT GCAAGGTAAC 
GACCGGACAC TGGGTCTGAG AGAGAACCTA CGCTGGAGGA AAGACCTTAC ACAGTGGCGC 
CAGACAAACG ACAGGCAGGA CAAATCCAAA GCAGAGCTGG ATCAAGAGGC CATAATCAGT 
GGGAATCTGG CGACTGAGAC CAACCTCATA GTGCTGGACC TGCTGGAGAT GATCGTACAG 
GCAGTTCCTC TGGCCGACTG TAAGGATAAT GTGGTTGGCG GAGTGTTGAA GGTTTTGCTG 
CACTCGCTCA CCTGTAATCA GAGCACCACC TACTTGTCGC ACTGTTTCAG CACTCTGAGG 
GCTCTCATTG TCAAGTTTGG CGACCTGTTG TTCGAGGAGG AGGCCGAGCA GTGTGCAGAC 
CTGTGCCAGA AGGTTCTTCA TTACTGCAGC AGTTGTGTGG ACGGCAACAG GAGTCAGGCT 
TGCGCTACGC TCTACCTGAT CATGAGATAC AGCTACAGCT CAGCTAGCAA CTTCTCCAGA 
GTCAAGATGC AGGTGACAAT GTCTTTGGCC TCACTGGTCG GCAAGTCTTC AGACATCCAC 
GAAGAGTATC TACGCCGCTC TTTCAGAACT ATCCTGGCAT ATGCTGAAGA GGACACCGAA 
ATGCAGTCTA CACAGCTGCC CTCACAGGTA GATGAACTCC TGAGGAACCT AAACAGTATC 
CTCTCAGATA CAGTCAAGAT GAAGGAGTTC CAGAAAGATC CCGAGATGCT TATGGACCTC 
ATGTATAGGA TCGCGAAGGG CTACCAGACT TCTCCAGACC TGCGTCTAAC CTGGCTTCAA 
AACATGGCAG AGAAACACAA CGGCAGGAAG TGCTTCACTG AGTCGGCCAT GTGTCTGGTT 
CACGCCGCAG CCCTGGTGGC TGAATATCTC AGCATGCTGG AGGATCACAA ATACTTGCCT 
GTGGGCAGCG TCACCTTTCA AAACATCTCT CCTAATGTGC TGGAGGAGTC TGCAGTCTCA 
GACGACATAT TGTCTCCAGA CGAGGATGGG GTTTGCTCAG GACGCTACTT CACCGAAAAT 
GGCCTAGTAG GGCTTCTGGA ACAGGCTGCC GAGCTATTCA GCAATGGCGG GTTGTTTGAG 
GCAGTTAATG AGGTGTACAA GGTAATCGTG CCTATACTGG AGGCCCACAG AGATTTCCGG 
AAACTCGCTT CCACACATGA CAAGCTCCAA AGGGCTTTTG ACAACATCAT TCAGAAGGGT 
CATAAGAGGA TGTTTGGAAC CTACTTCCGC GTGGGATTTT ATGGCTCTAA ATTTGGAGAC 
CTGGATGAGC AAGAATTTAT CTACAAAGAG CCAGGAATCA CCCATTTACC AGAGATATCC 
CACCGGCTCG AGAACTTCTA CAGTCAGTGC TTTGGTGATG GCGTTTTGGA AATGATTAAA 
GATTCCACAC CAGTAGACCG AAACAAGCTG AGTCCCAACA AGGCGTACAT ACAGATCACC 
TTTGTGGAGC CTTACTTTGA CGACTATGAG ATGAAAGACC GCCTCACCAA CTTTGAGAAG 
AACTTCAACC TTCGCCGCTT CATGTACACC ACGCCGTTCA CAAAGAGTGG GCGGCCCAGA 
GGAGAACTCA ACGAGCAATA CAAGCGGAAA ACCATCCTTA CCACTATGCA TGCCTTCCCC 
TACATCAAAA CACGAATTAA CGTCATTCAA AAGGAGGAGT TTGACCTCAC ACCAATCGAG 
GTGGCCATTG AGGACATGCA GAAGAAGACC CGAGAGCTGG CTGAAGCCAC ACACAGGGAG 
AAACCGGATG CTGTGATGCT TCAGATGGTT CTGCAGGGAT CTGTCACAGC CACTGTCAAT 
CAGGGTCCAT TGGAGGTGGC CCAGGTCTTC TTGAATGAAA TCCCAGCAGA CCCCAAGCTC 
TTCCGTCATC ACAACAAACT GCGCTTGTGT TTCAAAGAGT TCATACTGCG GTGTGGAGAG 
GCCATTGAGA AGAACAAGCA ACTCATCACT CCCGATCAGA AGGAGTACCA GCAGGAGATG 
AAGAAAAACT ACAATAAACT GCGAGAGAAC CTGAGGCCAA TGCTGGAGAG AAAGATCCCA 
GAGCTCTACA AGCCCATCAT CAAACCTCGC ATCGAGAACA GAGATTCCTT CAAACGGCAC 
AGCCTCCGTC GAACACAAGA TGAGCATTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001920107.4
CDS	203..6475
Translation	MASLPASERRAFALKINRHSSAEIRRHFSGVSHGSLPHHRHSYNASGSFCFNVPQSYEAVEPVDLDEFLMSQLRSGDAEVMQELGEFPDDDLDVELVERECRTIRPTIAEEGVELDSHVRDCVQSYTQPWLVVSRRSQGYGWISYSDRTDSNKILQRQTFESDVQPDKQEQLDKSPSLTALCDDSARTTLTSSDFNLRALQPDQRVESVLRFSSPEELDRFNQESRQSNRYGELFGLYPPIDEEDAVAIRPIPDCPKEHFGQRILVRCQTVKFEIEIEPLFATMALYDLKEKKKISENFNFDLNSDQMKGFLRTHTPQVDTSTLSRSAIFSISYPSPDIYLVIKIEKVLQQGEIGDCAEPYMVLKESDTTKNRDKLEKLRNQAEGFCQRLGRYRMPLAFATVNIMSVINSTLERETSDTDSINGRGSMDKKGLPRRNSDRFSLMEDNYILSGFKPAVTMCTFIKQEGERLSDEDLFKFLADIKRSSSQRRVKIIPGYLKLEVLPAPDTVPGCLSPELIPVKPVSEKNQRPIKEVLEFPSKEVYVPHNIYRNLLYVYPLRLNLTNRLTSARNITVKIQFMSGEDSSCAMPVIFGKSSGPEFLTEVYTPVTYHNRSPDFYDEIKILLPARLTDKHHLLFTFFHISCQQKQNQTGSVEALIGYSWLPMLNNDRLQTSQQCLPIVLDKLPINYSLHTPEKLPAQVPPVKWLENHKGLFNLELQAVSSVQTQDSHLERFFTLCHALEGKSTFPIRVRDEKIQENMFEHELKLSIISLSSAGLEPLVLFLHQVLDKLFRLIMQPMVIAGQTANLAQIAFESVVSVINSLHNSQELAKDHQGRNCLLATYLYFVFRLPDTQYEIHTSGTGGLMAHPEGRYSTLTRTTATTVGFMLLQSRIRSSSNPDIPAPLSPEDTEVNNILGKVLNHPGSRMSTAANASNSPQSSTGSTRPTNKKLFHEELALQMVVSTGVCRENVYKYAWFFFEVLVKSMSQHVSQLDKKTVSRRNRFSDRFKDDITTIVNVVTAEIGNILVKQQKELEQAEKVNISLAFFLYDLMSLMDRGFVFQLVKNYCNQMAAKSVNMATLISMRLEFLRVLCSHEHYLNLSLFFSSPSSAPASPSPSVSSQTSSSCSFQDNKIAAMFDLSQDFKQRHYLTGLLLTELSTALDIESEGGRVQQKAINATYSLLCAHDLDQRCSRSEVKAKIAALYLPLVGIIIDSINYLDFTVSDSRSGKGKSGGPEEDPDNIATINQSVAMAIAGNPFNTLTRNALVSMASVSVKTTNMLTAETSRNLLMCFLWIIKNADQNLIQRWTVDMPSSQLSRLLELLAICTSCFEYRGKQSSDKVSTQALQKSQQAKARLEEALLGGIGARGEMMKRVGVQGNDRTLGLRENLRWRKDLTQWRQTNDRQDKSKAELDQEAIISGNLATETNLIVLDLLEMIVQAVPLADCKDNVVGGVLKVLLHSLTCNQSTTYLSHCFSTLRALIVKFGDLLFEEEAEQCADLCQKVLHYCSSCVDGNRSQACATLYLIMRYSYSSASNFSRVKMQVTMSLASLVGKSSDIHEEYLRRSFRTILAYAEEDTEMQSTQLPSQVDELLRNLNSILSDTVKMKEFQKDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHNGRKCFTESAMCLVHAAALVAEYLSMLEDHKYLPVGSVTFQNISPNVLEESAVSDDILSPDEDGVCSGRYFTENGLVGLLEQAAELFSNGGLFEAVNEVYKVIVPILEAHRDFRKLASTHDKLQRAFDNIIQKGHKRMFGTYFRVGFYGSKFGDLDEQEFIYKEPGITHLPEISHRLENFYSQCFGDGVLEMIKDSTPVDRNKLSPNKAYIQITFVEPYFDDYEMKDRLTNFEKNFNLRRFMYTTPFTKSGRPRGELNEQYKRKTILTTMHAFPYIKTRINVIQKEEFDLTPIEVAIEDMQKKTRELAEATHREKPDAVMLQMVLQGSVTATVNQGPLEVAQVFLNEIPADPKLFRHHNKLRLCFKEFILRCGEAIEKNKQLITPDQKEYQQEMKKNYNKLRENLRPMLERKIPELYKPIIKPRIENRDSFKRHSLRRTQDEHS

Target ORF information:

RefSeq Version	XM_001920072.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001920072.6

ORF Insert Sequence:

ATGGCTTCTC TGCCGGCGTC AGAGCGGCGG GCGTTCGCGC TCAAAATCAA CAGACACTCA 
TCGGCAGAGA TCCGGAGACA CTTCAGTGGG GTCAGCCATG GTTCCCTGCC GCACCACCGG 
CACAGTTACA ACGCTTCTGG CTCTTTCTGC TTCAACGTAC CTCAATCATA TGAAGCGGTT 
GAGCCAGTGG ACCTGGATGA GTTCCTGATG TCTCAGCTGC GCAGTGGCGA TGCCGAGGTC 
ATGCAGGAGT TGGGTGAGTT TCCTGATGAC GACCTGGATG TTGAGCTTGT GGAGAGGGAA 
TGCAGGACCA TCCGCCCCAC TATTGCTGAG GAAGGAGTGG AGCTGGATTC CCATGTGAGG 
GATTGTGTTC AGAGTTACAC GCAGCCTTGG CTGGTGGTGA GCAGAAGAAG TCAGGGTTAT 
GGATGGATTT CATACTCTGA TAGAACAGAC TCTAACAAAA TCTTACAGAG GCAGACATTT 
GAGTCTGACG TTCAGCCAGA CAAACAGGAG CAACTAGATA AGTCGCCGTC GCTGACAGCA 
CTGTGTGATG ATTCAGCTCG GACCACCCTC ACAAGCTCAG ATTTTAACCT GAGGGCCCTT 
CAGCCAGACC AGCGAGTGGA AAGTGTCCTG CGTTTCAGCA GCCCAGAGGA ATTGGACCGC 
TTCAACCAGG AGTCCAGACA GAGCAACCGC TATGGCGAAC TCTTTGGCCT CTACCCGCCT 
ATTGATGAGG AGGATGCTGT TGCAATTCGG CCTATTCCTG ACTGTCCAAA GGAGCATTTT 
GGCCAAAGGA TATTAGTGCG ATGCCAGACC GTCAAATTTG AGATTGAAAT CGAGCCTCTC 
TTTGCAACAA TGGCCCTCTA TGACCTGAAG GAGAAGAAGA AGATTTCAGA GAACTTCAAC 
TTTGATCTGA ACTCAGATCA GATGAAGGGT TTCCTGCGGA CCCACACTCC TCAAGTTGAC 
ACTTCCACTT TGTCCAGATC TGCAATTTTT TCCATCTCCT ACCCCTCTCC AGACATCTAT 
CTGGTCATTA AGATTGAGAA GGTTCTTCAG CAGGGAGAGA TTGGGGATTG TGCTGAACCA 
TACATGGTAC TGAAGGAGAG TGACACGACT AAGAATAGAG ACAAGCTGGA GAAGTTGCGG 
AATCAGGCGG AGGGGTTTTG TCAAAGACTG GGTCGCTACA GGATGCCATT AGCCTTTGCT 
ACGGTCAACA TCATGAGTGT CATCAACTCC ACACTGGAAC GAGAGACAAG TGACACAGAC 
AGTATTAATG GCAGGGGGAG TATGGACAAG AAGGGATTGC CGAGACGTAA CTCTGACAGA 
TTCAGCTTGA TGGAGGACAA CTACATCCTG TCAGGCTTCA AACCAGCTGT CACTATGTGC 
ACATTCATCA AACAGGAAGG AGAACGTCTG AGTGACGAAG ATCTGTTTAA GTTCCTGGCG 
GATATAAAGA GGTCATCCAG TCAGAGACGT GTTAAAATCA TACCTGGTTA TCTGAAACTG 
GAAGTGCTTC CAGCGCCGGA CACGGTTCCT GGGTGTTTGT CTCCTGAACT GATTCCAGTG 
AAGCCTGTGT CTGAGAAAAA CCAACGTCCG ATTAAAGAAG TGCTCGAGTT CCCTTCGAAG 
GAAGTTTATG TGCCTCACAA TATATACCGG AATCTGCTGT ATGTATATCC GCTGAGGTTA 
AATCTTACTA ACCGTCTGAC TTCAGCCAGA AACATTACAG TAAAAATCCA GTTCATGAGC 
GGTGAGGACT CAAGTTGTGC CATGCCTGTA ATCTTTGGAA AATCAAGTGG CCCGGAGTTC 
CTTACGGAGG TCTACACTCC AGTCACTTAC CACAACAGGT CTCCTGATTT CTATGACGAG 
ATAAAGATCC TCCTGCCAGC CCGTTTAACA GATAAACACC ACTTGCTGTT CACCTTCTTC 
CATATCAGCT GCCAGCAGAA ACAAAACCAG ACTGGAAGTG TGGAGGCTCT CATTGGATAT 
TCATGGTTAC CCATGCTGAA CAATGACCGG CTGCAGACCA GCCAGCAGTG TCTGCCAATC 
GTTCTGGACA AGCTTCCTAT CAACTATTCC CTACATACCC CAGAGAAACT GCCAGCTCAG 
GTACCTCCAG TGAAGTGGCT GGAGAATCAT AAAGGACTGT TTAACCTTGA GCTACAAGCT 
GTGTCCTCAG TTCAAACTCA GGACAGTCAC CTGGAGCGTT TCTTCACATT ATGTCATGCT 
TTGGAGGGCA AATCCACATT CCCCATCCGA GTGCGAGATG AGAAGATTCA GGAAAACATG 
TTTGAGCATG AGCTGAAGCT CAGCATCATT TCACTGTCCT CCGCCGGCCT GGAGCCGCTG 
GTGCTTTTTC TCCATCAGGT CCTCGACAAG CTCTTCCGTC TCATCATGCA GCCCATGGTC 
ATCGCTGGAC AGACTGCAAA TCTGGCCCAA ATTGCGTTTG AGTCAGTGGT GTCGGTCATC 
AACAGTCTTC ACAACAGTCA GGAGCTGGCA AAAGACCATC AGGGCAGAAA CTGTCTCCTC 
GCAACATACC TGTACTTTGT GTTTCGCTTG CCAGATACTC AATATGAAAT CCACACCTCA 
GGTACAGGAG GTCTGATGGC TCATCCAGAG GGTCGATACA GCACTTTAAC TCGCACAACG 
GCCACCACTG TTGGCTTCAT GCTGCTGCAG TCACGAATCC GTTCCAGCAG CAACCCTGAT 
ATACCAGCAC CACTGAGCCC AGAGGACACA GAGGTCAATA ACATTCTAGG AAAGGTTTTA 
AATCATCCAG GCAGTCGCAT GTCAACAGCT GCAAATGCCT CCAATTCTCC GCAGAGCTCC 
ACGGGAAGCA CGCGGCCCAC CAACAAAAAG TTGTTCCACG AGGAGCTGGC TCTTCAGATG 
GTGGTCAGCA CCGGAGTCTG CAGAGAAAAT GTCTACAAAT ACGCCTGGTT CTTCTTTGAA 
GTTCTGGTGA AGAGCATGAG TCAGCACGTG TCTCAGCTGG ATAAGAAGAC GGTCTCTCGC 
AGGAATCGAT TTTCCGACCG TTTCAAAGAT GACATCACCA CCATTGTCAA CGTGGTGACC 
GCTGAGATCG GGAACATTTT AGTAAAGCAG CAAAAGGAGC TGGAGCAGGC AGAGAAGGTT 
AACATCAGCC TGGCTTTCTT CCTGTATGAC CTGATGTCTC TGATGGATCG AGGCTTTGTG 
TTTCAGCTGG TGAAGAATTA CTGCAACCAG ATGGCAGCAA AGAGTGTGAA CATGGCGACT 
TTGATAAGTA TGAGGCTGGA GTTTTTGAGG GTCTTGTGCA GTCATGAGCA CTACTTGAAC 
CTCAGTCTCT TCTTCAGCAG CCCTTCATCT GCCCCTGCCT CCCCATCACC CTCGGTGTCC 
TCACAGACGT CCAGCTCGTG CAGTTTTCAG GACAACAAGA TCGCAGCCAT GTTTGATTTG 
TCGCAGGACT TTAAGCAGCG CCATTACTTG ACCGGACTCT TGCTAACTGA ACTCAGCACT 
GCACTGGACA TAGAGTCCGA GGGGGGCAGA GTTCAACAAA AAGCCATCAA TGCCACATAC 
AGTCTATTAT GTGCTCATGA TCTGGACCAG CGCTGCTCAA GGTCAGAGGT CAAGGCGAAG 
ATTGCTGCTC TCTACCTCCC GCTAGTGGGC ATCATTATTG ACTCCATCAA TTACCTGGAC 
TTCACAGTTT CTGACTCTCG TAGTGGTAAA GGCAAGTCTG GTGGGCCTGA GGAAGACCCT 
GACAACATTG CCACCATCAA TCAGTCTGTT GCAATGGCAA TCGCTGGAAA TCCTTTCAAC 
ACCTTAACCA GGAACGCACT GGTGTCTATG GCCTCTGTGA GTGTGAAAAC CACCAACATG 
TTGACGGCGG AGACCAGTCG CAATCTGCTG ATGTGTTTCC TGTGGATCAT CAAGAACGCA 
GACCAGAATC TGATCCAGCG CTGGACTGTG GACATGCCTT CATCTCAGCT GAGTCGACTG 
CTGGAGCTCC TCGCCATCTG CACCTCCTGC TTCGAGTACA GGGGGAAGCA GAGCTCTGAT 
AAGGTGAGCA CTCAGGCACT CCAGAAGTCT CAGCAGGCCA AGGCGCGTCT GGAGGAGGCT 
CTGCTGGGAG GAATCGGAGC CAGAGGAGAA ATGATGAAAC GGGTGGGAGT GCAAGGTAAC 
GACCGGACAC TGGGTCTGAG AGAGAACCTA CGCTGGAGGA AAGACCTTAC ACAGTGGCGC 
CAGACAAACG ACAGGCAGGA CAAATCCAAA GCAGAGCTGG ATCAAGAGGC CATAATCAGT 
GGGAATCTGG CGACTGAGAC CAACCTCATA GTGCTGGACC TGCTGGAGAT GATCGTACAG 
GCAGTTCCTC TGGCCGACTG TAAGGATAAT GTGGTTGGCG GAGTGTTGAA GGTTTTGCTG 
CACTCGCTCA CCTGTAATCA GAGCACCACC TACTTGTCGC ACTGTTTCAG CACTCTGAGG 
GCTCTCATTG TCAAGTTTGG CGACCTGTTG TTCGAGGAGG AGGCCGAGCA GTGTGCAGAC 
CTGTGCCAGA AGGTTCTTCA TTACTGCAGC AGTTGTGTGG ACGGCAACAG GAGTCAGGCT 
TGCGCTACGC TCTACCTGAT CATGAGATAC AGCTACAGCT CAGCTAGCAA CTTCTCCAGA 
GTCAAGATGC AGGTGACAAT GTCTTTGGCC TCACTGGTCG GCAAGTCTTC AGACATCCAC 
GAAGAGTATC TACGCCGCTC TTTCAGAACT ATCCTGGCAT ATGCTGAAGA GGACACCGAA 
ATGCAGTCTA CACAGCTGCC CTCACAGGTA GATGAACTCC TGAGGAACCT AAACAGTATC 
CTCTCAGATA CAGTCAAGAT GAAGGAGTTC CAGAAAGATC CCGAGATGCT TATGGACCTC 
ATGTATAGGA TCGCGAAGGG CTACCAGACT TCTCCAGACC TGCGTCTAAC CTGGCTTCAA 
AACATGGCAG AGAAACACAA CGGCAGGAAG TGCTTCACTG AGTCGGCCAT GTGTCTGGTT 
CACGCCGCAG CCCTGGTGGC TGAATATCTC AGCATGCTGG AGGATCACAA ATACTTGCCT 
GTGGGCAGCG TCACCTTTCA AAACATCTCT CCTAATGTGC TGGAGGAGTC TGCAGTCTCA 
GACGACATAT TGTCTCCAGA CGAGGATGGG GTTTGCTCAG GACGCTACTT CACCGAAAAT 
GGCCTAGTAG GGCTTCTGGA ACAGGCTGCC GAGCTATTCA GCAATGGCGG GTTGTTTGAG 
GCAGTTAATG AGGTGTACAA GGTAATCGTG CCTATACTGG AGGCCCACAG AGATTTCCGG 
AAACTCGCTT CCACACATGA CAAGCTCCAA AGGGCTTTTG ACAACATCAT TCAGAAGGGT 
CATAAGAGGA TGTTTGGAAC CTACTTCCGC GTGGGATTTT ATGGCTCTAA ATTTGGAGAC 
CTGGATGAGC AAGAATTTAT CTACAAAGAG CCAGGAATCA CCCATTTACC AGAGATATCC 
CACCGGCTCG AGAACTTCTA CAGTCAGTGC TTTGGTGATG GCGTTTTGGA AATGATTAAA 
GATTCCACAC CAGTAGACCG AAACAAGCTG AGTCCCAACA AGGCGTACAT ACAGATCACC 
TTTGTGGAGC CTTACTTTGA CGACTATGAG ATGAAAGACC GCCTCACCAA CTTTGAGAAG 
AACTTCAACC TTCGCCGCTT CATGTACACC ACGCCGTTCA CAAAGAGTGG GCGGCCCAGA 
GGAGAACTCA ACGAGCAATA CAAGCGGAAA ACCATCCTTA CCACTATGCA TGCCTTCCCC 
TACATCAAAA CACGAATTAA CGTCATTCAA AAGGAGGAGT TTGACCTCAC ACCAATCGAG 
GTGGCCATTG AGGACATGCA GAAGAAGACC CGAGAGCTGG CTGAAGCCAC ACACAGGGAG 
AAACCGGATG CTGTGATGCT TCAGATGGTT CTGCAGGGAT CTGTCACAGC CACTGTCAAT 
CAGGGTCCAT TGGAGGTGGC CCAGGTCTTC TTGAATGAAA TCCCAGCAGA CCCCAAGCTC 
TTCCGTCATC ACAACAAACT GCGCTTGTGT TTCAAAGAGT TCATACTGCG GTGTGGAGAG 
GCCATTGAGA AGAACAAGCA ACTCATCACT CCCGATCAGA AGGAGTACCA GCAGGAGATG 
AAGAAAAACT ACAATAAACT GCGAGAGAAC CTGAGGCCAA TGCTGGAGAG AAAGATCCCA 
GAGCTCTACA AGCCCATCAT CAAACCTCGC ATCGAGAACA GAGATTCCTT CAAACGGCAC 
AGCCTCCGTC GAACACAAGA TGAGCATTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_001920072.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001920107.4 Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

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CloneID	ODa14388
Clone ID Related Accession (Same CDS sequence)	XM_001920072.6 , XM_001920072.7
Accession Version	XM_001920072.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001920072.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCTC TGCCGGCGTC AGAGCGGCGG GCGTTCGCGC TCAAAATCAA CAGACACTCA 
TCGGCAGAGA TCCGGAGACA CTTCAGTGGG GTCAGCCATG GTTCCCTGCC GCACCACCGG 
CACAGTTACA ACGCTTCTGG CTCTTTCTGC TTCAACGTAC CTCAATCATA TGAAGCGGTT 
GAGCCAGTGG ACCTGGATGA GTTCCTGATG TCTCAGCTGC GCAGTGGCGA TGCCGAGGTC 
ATGCAGGAGT TGGGTGAGTT TCCTGATGAC GACCTGGATG TTGAGCTTGT GGAGAGGGAA 
TGCAGGACCA TCCGCCCCAC TATTGCTGAG GAAGGAGTGG AGCTGGATTC CCATGTGAGG 
GATTGTGTTC AGAGTTACAC GCAGCCTTGG CTGGTGGTGA GCAGAAGAAG TCAGGGTTAT 
GGATGGATTT CATACTCTGA TAGAACAGAC TCTAACAAAA TCTTACAGAG GCAGACATTT 
GAGTCTGACG TTCAGCCAGA CAAACAGGAG CAACTAGATA AGTCGCCGTC GCTGACAGCA 
CTGTGTGATG ATTCAGCTCG GACCACCCTC ACAAGCTCAG ATTTTAACCT GAGGGCCCTT 
CAGCCAGACC AGCGAGTGGA AAGTGTCCTG CGTTTCAGCA GCCCAGAGGA ATTGGACCGC 
TTCAACCAGG AGTCCAGACA GAGCAACCGC TATGGCGAAC TCTTTGGCCT CTACCCGCCT 
ATTGATGAGG AGGATGCTGT TGCAATTCGG CCTATTCCTG ACTGTCCAAA GGAGCATTTT 
GGCCAAAGGA TATTAGTGCG ATGCCAGACC GTCAAATTTG AGATTGAAAT CGAGCCTCTC 
TTTGCAACAA TGGCCCTCTA TGACCTGAAG GAGAAGAAGA AGATTTCAGA GAACTTCAAC 
TTTGATCTGA ACTCAGATCA GATGAAGGGT TTCCTGCGGA CCCACACTCC TCAAGTTGAC 
ACTTCCACTT TGTCCAGATC TGCAATTTTT TCCATCTCCT ACCCCTCTCC AGACATCTAT 
CTGGTCATTA AGATTGAGAA GGTTCTTCAG CAGGGAGAGA TTGGGGATTG TGCTGAACCA 
TACATGGTAC TGAAGGAGAG TGACACGACT AAGAATAGAG ACAAGCTGGA GAAGTTGCGG 
AATCAGGCGG AGGGGTTTTG TCAAAGACTG GGTCGCTACA GGATGCCATT AGCCTTTGCT 
ACGGTCAACA TCATGAGTGT CATCAACTCC ACACTGGAAC GAGAGACAAG TGACACAGAC 
AGTATTAATG GCAGGGGGAG TATGGACAAG AAGGGATTGC CGAGACGTAA CTCTGACAGA 
TTCAGCTTGA TGGAGGACAA CTACATCCTG TCAGGCTTCA AACCAGCTGT CACTATGTGC 
ACATTCATCA AACAGGAAGG AGAACGTCTG AGTGACGAAG ATCTGTTTAA GTTCCTGGCG 
GATATAAAGA GGTCATCCAG TCAGAGACGT GTTAAAATCA TACCTGGTTA TCTGAAACTG 
GAAGTGCTTC CAGCGCCGGA CACGGTTCCT GGGTGTTTGT CTCCTGAACT GATTCCAGTG 
AAGCCTGTGT CTGAGAAAAA CCAACGTCCG ATTAAAGAAG TGCTCGAGTT CCCTTCGAAG 
GAAGTTTATG TGCCTCACAA TATATACCGG AATCTGCTGT ATGTATATCC GCTGAGGTTA 
AATCTTACTA ACCGTCTGAC TTCAGCCAGA AACATTACAG TAAAAATCCA GTTCATGAGC 
GGTGAGGACT CAAGTTGTGC CATGCCTGTA ATCTTTGGAA AATCAAGTGG CCCGGAGTTC 
CTTACGGAGG TCTACACTCC AGTCACTTAC CACAACAGGT CTCCTGATTT CTATGACGAG 
ATAAAGATCC TCCTGCCAGC CCGTTTAACA GATAAACACC ACTTGCTGTT CACCTTCTTC 
CATATCAGCT GCCAGCAGAA ACAAAACCAG ACTGGAAGTG TGGAGGCTCT CATTGGATAT 
TCATGGTTAC CCATGCTGAA CAATGACCGG CTGCAGACCA GCCAGCAGTG TCTGCCAATC 
GTTCTGGACA AGCTTCCTAT CAACTATTCC CTACATACCC CAGAGAAACT GCCAGCTCAG 
GTACCTCCAG TGAAGTGGCT GGAGAATCAT AAAGGACTGT TTAACCTTGA GCTACAAGCT 
GTGTCCTCAG TTCAAACTCA GGACAGTCAC CTGGAGCGTT TCTTCACATT ATGTCATGCT 
TTGGAGGGCA AATCCACATT CCCCATCCGA GTGCGAGATG AGAAGATTCA GGAAAACATG 
TTTGAGCATG AGCTGAAGCT CAGCATCATT TCACTGTCCT CCGCCGGCCT GGAGCCGCTG 
GTGCTTTTTC TCCATCAGGT CCTCGACAAG CTCTTCCGTC TCATCATGCA GCCCATGGTC 
ATCGCTGGAC AGACTGCAAA TCTGGCCCAA ATTGCGTTTG AGTCAGTGGT GTCGGTCATC 
AACAGTCTTC ACAACAGTCA GGAGCTGGCA AAAGACCATC AGGGCAGAAA CTGTCTCCTC 
GCAACATACC TGTACTTTGT GTTTCGCTTG CCAGATACTC AATATGAAAT CCACACCTCA 
GGTACAGGAG GTCTGATGGC TCATCCAGAG GGTCGATACA GCACTTTAAC TCGCACAACG 
GCCACCACTG TTGGCTTCAT GCTGCTGCAG TCACGAATCC GTTCCAGCAG CAACCCTGAT 
ATACCAGCAC CACTGAGCCC AGAGGACACA GAGGTCAATA ACATTCTAGG AAAGGTTTTA 
AATCATCCAG GCAGTCGCAT GTCAACAGCT GCAAATGCCT CCAATTCTCC GCAGAGCTCC 
ACGGGAAGCA CGCGGCCCAC CAACAAAAAG TTGTTCCACG AGGAGCTGGC TCTTCAGATG 
GTGGTCAGCA CCGGAGTCTG CAGAGAAAAT GTCTACAAAT ACGCCTGGTT CTTCTTTGAA 
GTTCTGGTGA AGAGCATGAG TCAGCACGTG TCTCAGCTGG ATAAGAAGAC GGTCTCTCGC 
AGGAATCGAT TTTCCGACCG TTTCAAAGAT GACATCACCA CCATTGTCAA CGTGGTGACC 
GCTGAGATCG GGAACATTTT AGTAAAGCAG CAAAAGGAGC TGGAGCAGGC AGAGAAGGTT 
AACATCAGCC TGGCTTTCTT CCTGTATGAC CTGATGTCTC TGATGGATCG AGGCTTTGTG 
TTTCAGCTGG TGAAGAATTA CTGCAACCAG ATGGCAGCAA AGAGTGTGAA CATGGCGACT 
TTGATAAGTA TGAGGCTGGA GTTTTTGAGG GTCTTGTGCA GTCATGAGCA CTACTTGAAC 
CTCAGTCTCT TCTTCAGCAG CCCTTCATCT GCCCCTGCCT CCCCATCACC CTCGGTGTCC 
TCACAGACGT CCAGCTCGTG CAGTTTTCAG GACAACAAGA TCGCAGCCAT GTTTGATTTG 
TCGCAGGACT TTAAGCAGCG CCATTACTTG ACCGGACTCT TGCTAACTGA ACTCAGCACT 
GCACTGGACA TAGAGTCCGA GGGGGGCAGA GTTCAACAAA AAGCCATCAA TGCCACATAC 
AGTCTATTAT GTGCTCATGA TCTGGACCAG CGCTGCTCAA GGTCAGAGGT CAAGGCGAAG 
ATTGCTGCTC TCTACCTCCC GCTAGTGGGC ATCATTATTG ACTCCATCAA TTACCTGGAC 
TTCACAGTTT CTGACTCTCG TAGTGGTAAA GGCAAGTCTG GTGGGCCTGA GGAAGACCCT 
GACAACATTG CCACCATCAA TCAGTCTGTT GCAATGGCAA TCGCTGGAAA TCCTTTCAAC 
ACCTTAACCA GGAACGCACT GGTGTCTATG GCCTCTGTGA GTGTGAAAAC CACCAACATG 
TTGACGGCGG AGACCAGTCG CAATCTGCTG ATGTGTTTCC TGTGGATCAT CAAGAACGCA 
GACCAGAATC TGATCCAGCG CTGGACTGTG GACATGCCTT CATCTCAGCT GAGTCGACTG 
CTGGAGCTCC TCGCCATCTG CACCTCCTGC TTCGAGTACA GGGGGAAGCA GAGCTCTGAT 
AAGGTGAGCA CTCAGGCACT CCAGAAGTCT CAGCAGGCCA AGGCGCGTCT GGAGGAGGCT 
CTGCTGGGAG GAATCGGAGC CAGAGGAGAA ATGATGAAAC GGGTGGGAGT GCAAGGTAAC 
GACCGGACAC TGGGTCTGAG AGAGAACCTA CGCTGGAGGA AAGACCTTAC ACAGTGGCGC 
CAGACAAACG ACAGGCAGGA CAAATCCAAA GCAGAGCTGG ATCAAGAGGC CATAATCAGT 
GGGAATCTGG CGACTGAGAC CAACCTCATA GTGCTGGACC TGCTGGAGAT GATCGTACAG 
GCAGTTCCTC TGGCCGACTG TAAGGATAAT GTGGTTGGCG GAGTGTTGAA GGTTTTGCTG 
CACTCGCTCA CCTGTAATCA GAGCACCACC TACTTGTCGC ACTGTTTCAG CACTCTGAGG 
GCTCTCATTG TCAAGTTTGG CGACCTGTTG TTCGAGGAGG AGGCCGAGCA GTGTGCAGAC 
CTGTGCCAGA AGGTTCTTCA TTACTGCAGC AGTTGTGTGG ACGGCAACAG GAGTCAGGCT 
TGCGCTACGC TCTACCTGAT CATGAGATAC AGCTACAGCT CAGCTAGCAA CTTCTCCAGA 
GTCAAGATGC AGGTGACAAT GTCTTTGGCC TCACTGGTCG GCAAGTCTTC AGACATCCAC 
GAAGAGTATC TACGCCGCTC TTTCAGAACT ATCCTGGCAT ATGCTGAAGA GGACACCGAA 
ATGCAGTCTA CACAGCTGCC CTCACAGGTA GATGAACTCC TGAGGAACCT AAACAGTATC 
CTCTCAGATA CAGTCAAGAT GAAGGAGTTC CAGAAAGATC CCGAGATGCT TATGGACCTC 
ATGTATAGGA TCGCGAAGGG CTACCAGACT TCTCCAGACC TGCGTCTAAC CTGGCTTCAA 
AACATGGCAG AGAAACACAA CGGCAGGAAG TGCTTCACTG AGTCGGCCAT GTGTCTGGTT 
CACGCCGCAG CCCTGGTGGC TGAATATCTC AGCATGCTGG AGGATCACAA ATACTTGCCT 
GTGGGCAGCG TCACCTTTCA AAACATCTCT CCTAATGTGC TGGAGGAGTC TGCAGTCTCA 
GACGACATAT TGTCTCCAGA CGAGGATGGG GTTTGCTCAG GACGCTACTT CACCGAAAAT 
GGCCTAGTAG GGCTTCTGGA ACAGGCTGCC GAGCTATTCA GCAATGGCGG GTTGTTTGAG 
GCAGTTAATG AGGTGTACAA GGTAATCGTG CCTATACTGG AGGCCCACAG AGATTTCCGG 
AAACTCGCTT CCACACATGA CAAGCTCCAA AGGGCTTTTG ACAACATCAT TCAGAAGGGT 
CATAAGAGGA TGTTTGGAAC CTACTTCCGC GTGGGATTTT ATGGCTCTAA ATTTGGAGAC 
CTGGATGAGC AAGAATTTAT CTACAAAGAG CCAGGAATCA CCCATTTACC AGAGATATCC 
CACCGGCTCG AGAACTTCTA CAGTCAGTGC TTTGGTGATG GCGTTTTGGA AATGATTAAA 
GATTCCACAC CAGTAGACCG AAACAAGCTG AGTCCCAACA AGGCGTACAT ACAGATCACC 
TTTGTGGAGC CTTACTTTGA CGACTATGAG ATGAAAGACC GCCTCACCAA CTTTGAGAAG 
AACTTCAACC TTCGCCGCTT CATGTACACC ACGCCGTTCA CAAAGAGTGG GCGGCCCAGA 
GGAGAACTCA ACGAGCAATA CAAGCGGAAA ACCATCCTTA CCACTATGCA TGCCTTCCCC 
TACATCAAAA CACGAATTAA CGTCATTCAA AAGGAGGAGT TTGACCTCAC ACCAATCGAG 
GTGGCCATTG AGGACATGCA GAAGAAGACC CGAGAGCTGG CTGAAGCCAC ACACAGGGAG 
AAACCGGATG CTGTGATGCT TCAGATGGTT CTGCAGGGAT CTGTCACAGC CACTGTCAAT 
CAGGGTCCAT TGGAGGTGGC CCAGGTCTTC TTGAATGAAA TCCCAGCAGA CCCCAAGCTC 
TTCCGTCATC ACAACAAACT GCGCTTGTGT TTCAAAGAGT TCATACTGCG GTGTGGAGAG 
GCCATTGAGA AGAACAAGCA ACTCATCACT CCCGATCAGA AGGAGTACCA GCAGGAGATG 
AAGAAAAACT ACAATAAACT GCGAGAGAAC CTGAGGCCAA TGCTGGAGAG AAAGATCCCA 
GAGCTCTACA AGCCCATCAT CAAACCTCGC ATCGAGAACA GAGATTCCTT CAAACGGCAC 
AGCCTCCGTC GAACACAAGA TGAGCATTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001920107.4
CDS	189..6461
Translation	MASLPASERRAFALKINRHSSAEIRRHFSGVSHGSLPHHRHSYNASGSFCFNVPQSYEAVEPVDLDEFLMSQLRSGDAEVMQELGEFPDDDLDVELVERECRTIRPTIAEEGVELDSHVRDCVQSYTQPWLVVSRRSQGYGWISYSDRTDSNKILQRQTFESDVQPDKQEQLDKSPSLTALCDDSARTTLTSSDFNLRALQPDQRVESVLRFSSPEELDRFNQESRQSNRYGELFGLYPPIDEEDAVAIRPIPDCPKEHFGQRILVRCQTVKFEIEIEPLFATMALYDLKEKKKISENFNFDLNSDQMKGFLRTHTPQVDTSTLSRSAIFSISYPSPDIYLVIKIEKVLQQGEIGDCAEPYMVLKESDTTKNRDKLEKLRNQAEGFCQRLGRYRMPLAFATVNIMSVINSTLERETSDTDSINGRGSMDKKGLPRRNSDRFSLMEDNYILSGFKPAVTMCTFIKQEGERLSDEDLFKFLADIKRSSSQRRVKIIPGYLKLEVLPAPDTVPGCLSPELIPVKPVSEKNQRPIKEVLEFPSKEVYVPHNIYRNLLYVYPLRLNLTNRLTSARNITVKIQFMSGEDSSCAMPVIFGKSSGPEFLTEVYTPVTYHNRSPDFYDEIKILLPARLTDKHHLLFTFFHISCQQKQNQTGSVEALIGYSWLPMLNNDRLQTSQQCLPIVLDKLPINYSLHTPEKLPAQVPPVKWLENHKGLFNLELQAVSSVQTQDSHLERFFTLCHALEGKSTFPIRVRDEKIQENMFEHELKLSIISLSSAGLEPLVLFLHQVLDKLFRLIMQPMVIAGQTANLAQIAFESVVSVINSLHNSQELAKDHQGRNCLLATYLYFVFRLPDTQYEIHTSGTGGLMAHPEGRYSTLTRTTATTVGFMLLQSRIRSSSNPDIPAPLSPEDTEVNNILGKVLNHPGSRMSTAANASNSPQSSTGSTRPTNKKLFHEELALQMVVSTGVCRENVYKYAWFFFEVLVKSMSQHVSQLDKKTVSRRNRFSDRFKDDITTIVNVVTAEIGNILVKQQKELEQAEKVNISLAFFLYDLMSLMDRGFVFQLVKNYCNQMAAKSVNMATLISMRLEFLRVLCSHEHYLNLSLFFSSPSSAPASPSPSVSSQTSSSCSFQDNKIAAMFDLSQDFKQRHYLTGLLLTELSTALDIESEGGRVQQKAINATYSLLCAHDLDQRCSRSEVKAKIAALYLPLVGIIIDSINYLDFTVSDSRSGKGKSGGPEEDPDNIATINQSVAMAIAGNPFNTLTRNALVSMASVSVKTTNMLTAETSRNLLMCFLWIIKNADQNLIQRWTVDMPSSQLSRLLELLAICTSCFEYRGKQSSDKVSTQALQKSQQAKARLEEALLGGIGARGEMMKRVGVQGNDRTLGLRENLRWRKDLTQWRQTNDRQDKSKAELDQEAIISGNLATETNLIVLDLLEMIVQAVPLADCKDNVVGGVLKVLLHSLTCNQSTTYLSHCFSTLRALIVKFGDLLFEEEAEQCADLCQKVLHYCSSCVDGNRSQACATLYLIMRYSYSSASNFSRVKMQVTMSLASLVGKSSDIHEEYLRRSFRTILAYAEEDTEMQSTQLPSQVDELLRNLNSILSDTVKMKEFQKDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHNGRKCFTESAMCLVHAAALVAEYLSMLEDHKYLPVGSVTFQNISPNVLEESAVSDDILSPDEDGVCSGRYFTENGLVGLLEQAAELFSNGGLFEAVNEVYKVIVPILEAHRDFRKLASTHDKLQRAFDNIIQKGHKRMFGTYFRVGFYGSKFGDLDEQEFIYKEPGITHLPEISHRLENFYSQCFGDGVLEMIKDSTPVDRNKLSPNKAYIQITFVEPYFDDYEMKDRLTNFEKNFNLRRFMYTTPFTKSGRPRGELNEQYKRKTILTTMHAFPYIKTRINVIQKEEFDLTPIEVAIEDMQKKTRELAEATHREKPDAVMLQMVLQGSVTATVNQGPLEVAQVFLNEIPADPKLFRHHNKLRLCFKEFILRCGEAIEKNKQLITPDQKEYQQEMKKNYNKLRENLRPMLERKIPELYKPIIKPRIENRDSFKRHSLRRTQDEHS

Target ORF information:

RefSeq Version	XM_001920072.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001920072.7

ORF Insert Sequence:

ATGGCTTCTC TGCCGGCGTC AGAGCGGCGG GCGTTCGCGC TCAAAATCAA CAGACACTCA 
TCGGCAGAGA TCCGGAGACA CTTCAGTGGG GTCAGCCATG GTTCCCTGCC GCACCACCGG 
CACAGTTACA ACGCTTCTGG CTCTTTCTGC TTCAACGTAC CTCAATCATA TGAAGCGGTT 
GAGCCAGTGG ACCTGGATGA GTTCCTGATG TCTCAGCTGC GCAGTGGCGA TGCCGAGGTC 
ATGCAGGAGT TGGGTGAGTT TCCTGATGAC GACCTGGATG TTGAGCTTGT GGAGAGGGAA 
TGCAGGACCA TCCGCCCCAC TATTGCTGAG GAAGGAGTGG AGCTGGATTC CCATGTGAGG 
GATTGTGTTC AGAGTTACAC GCAGCCTTGG CTGGTGGTGA GCAGAAGAAG TCAGGGTTAT 
GGATGGATTT CATACTCTGA TAGAACAGAC TCTAACAAAA TCTTACAGAG GCAGACATTT 
GAGTCTGACG TTCAGCCAGA CAAACAGGAG CAACTAGATA AGTCGCCGTC GCTGACAGCA 
CTGTGTGATG ATTCAGCTCG GACCACCCTC ACAAGCTCAG ATTTTAACCT GAGGGCCCTT 
CAGCCAGACC AGCGAGTGGA AAGTGTCCTG CGTTTCAGCA GCCCAGAGGA ATTGGACCGC 
TTCAACCAGG AGTCCAGACA GAGCAACCGC TATGGCGAAC TCTTTGGCCT CTACCCGCCT 
ATTGATGAGG AGGATGCTGT TGCAATTCGG CCTATTCCTG ACTGTCCAAA GGAGCATTTT 
GGCCAAAGGA TATTAGTGCG ATGCCAGACC GTCAAATTTG AGATTGAAAT CGAGCCTCTC 
TTTGCAACAA TGGCCCTCTA TGACCTGAAG GAGAAGAAGA AGATTTCAGA GAACTTCAAC 
TTTGATCTGA ACTCAGATCA GATGAAGGGT TTCCTGCGGA CCCACACTCC TCAAGTTGAC 
ACTTCCACTT TGTCCAGATC TGCAATTTTT TCCATCTCCT ACCCCTCTCC AGACATCTAT 
CTGGTCATTA AGATTGAGAA GGTTCTTCAG CAGGGAGAGA TTGGGGATTG TGCTGAACCA 
TACATGGTAC TGAAGGAGAG TGACACGACT AAGAATAGAG ACAAGCTGGA GAAGTTGCGG 
AATCAGGCGG AGGGGTTTTG TCAAAGACTG GGTCGCTACA GGATGCCATT AGCCTTTGCT 
ACGGTCAACA TCATGAGTGT CATCAACTCC ACACTGGAAC GAGAGACAAG TGACACAGAC 
AGTATTAATG GCAGGGGGAG TATGGACAAG AAGGGATTGC CGAGACGTAA CTCTGACAGA 
TTCAGCTTGA TGGAGGACAA CTACATCCTG TCAGGCTTCA AACCAGCTGT CACTATGTGC 
ACATTCATCA AACAGGAAGG AGAACGTCTG AGTGACGAAG ATCTGTTTAA GTTCCTGGCG 
GATATAAAGA GGTCATCCAG TCAGAGACGT GTTAAAATCA TACCTGGTTA TCTGAAACTG 
GAAGTGCTTC CAGCGCCGGA CACGGTTCCT GGGTGTTTGT CTCCTGAACT GATTCCAGTG 
AAGCCTGTGT CTGAGAAAAA CCAACGTCCG ATTAAAGAAG TGCTCGAGTT CCCTTCGAAG 
GAAGTTTATG TGCCTCACAA TATATACCGG AATCTGCTGT ATGTATATCC GCTGAGGTTA 
AATCTTACTA ACCGTCTGAC TTCAGCCAGA AACATTACAG TAAAAATCCA GTTCATGAGC 
GGTGAGGACT CAAGTTGTGC CATGCCTGTA ATCTTTGGAA AATCAAGTGG CCCGGAGTTC 
CTTACGGAGG TCTACACTCC AGTCACTTAC CACAACAGGT CTCCTGATTT CTATGACGAG 
ATAAAGATCC TCCTGCCAGC CCGTTTAACA GATAAACACC ACTTGCTGTT CACCTTCTTC 
CATATCAGCT GCCAGCAGAA ACAAAACCAG ACTGGAAGTG TGGAGGCTCT CATTGGATAT 
TCATGGTTAC CCATGCTGAA CAATGACCGG CTGCAGACCA GCCAGCAGTG TCTGCCAATC 
GTTCTGGACA AGCTTCCTAT CAACTATTCC CTACATACCC CAGAGAAACT GCCAGCTCAG 
GTACCTCCAG TGAAGTGGCT GGAGAATCAT AAAGGACTGT TTAACCTTGA GCTACAAGCT 
GTGTCCTCAG TTCAAACTCA GGACAGTCAC CTGGAGCGTT TCTTCACATT ATGTCATGCT 
TTGGAGGGCA AATCCACATT CCCCATCCGA GTGCGAGATG AGAAGATTCA GGAAAACATG 
TTTGAGCATG AGCTGAAGCT CAGCATCATT TCACTGTCCT CCGCCGGCCT GGAGCCGCTG 
GTGCTTTTTC TCCATCAGGT CCTCGACAAG CTCTTCCGTC TCATCATGCA GCCCATGGTC 
ATCGCTGGAC AGACTGCAAA TCTGGCCCAA ATTGCGTTTG AGTCAGTGGT GTCGGTCATC 
AACAGTCTTC ACAACAGTCA GGAGCTGGCA AAAGACCATC AGGGCAGAAA CTGTCTCCTC 
GCAACATACC TGTACTTTGT GTTTCGCTTG CCAGATACTC AATATGAAAT CCACACCTCA 
GGTACAGGAG GTCTGATGGC TCATCCAGAG GGTCGATACA GCACTTTAAC TCGCACAACG 
GCCACCACTG TTGGCTTCAT GCTGCTGCAG TCACGAATCC GTTCCAGCAG CAACCCTGAT 
ATACCAGCAC CACTGAGCCC AGAGGACACA GAGGTCAATA ACATTCTAGG AAAGGTTTTA 
AATCATCCAG GCAGTCGCAT GTCAACAGCT GCAAATGCCT CCAATTCTCC GCAGAGCTCC 
ACGGGAAGCA CGCGGCCCAC CAACAAAAAG TTGTTCCACG AGGAGCTGGC TCTTCAGATG 
GTGGTCAGCA CCGGAGTCTG CAGAGAAAAT GTCTACAAAT ACGCCTGGTT CTTCTTTGAA 
GTTCTGGTGA AGAGCATGAG TCAGCACGTG TCTCAGCTGG ATAAGAAGAC GGTCTCTCGC 
AGGAATCGAT TTTCCGACCG TTTCAAAGAT GACATCACCA CCATTGTCAA CGTGGTGACC 
GCTGAGATCG GGAACATTTT AGTAAAGCAG CAAAAGGAGC TGGAGCAGGC AGAGAAGGTT 
AACATCAGCC TGGCTTTCTT CCTGTATGAC CTGATGTCTC TGATGGATCG AGGCTTTGTG 
TTTCAGCTGG TGAAGAATTA CTGCAACCAG ATGGCAGCAA AGAGTGTGAA CATGGCGACT 
TTGATAAGTA TGAGGCTGGA GTTTTTGAGG GTCTTGTGCA GTCATGAGCA CTACTTGAAC 
CTCAGTCTCT TCTTCAGCAG CCCTTCATCT GCCCCTGCCT CCCCATCACC CTCGGTGTCC 
TCACAGACGT CCAGCTCGTG CAGTTTTCAG GACAACAAGA TCGCAGCCAT GTTTGATTTG 
TCGCAGGACT TTAAGCAGCG CCATTACTTG ACCGGACTCT TGCTAACTGA ACTCAGCACT 
GCACTGGACA TAGAGTCCGA GGGGGGCAGA GTTCAACAAA AAGCCATCAA TGCCACATAC 
AGTCTATTAT GTGCTCATGA TCTGGACCAG CGCTGCTCAA GGTCAGAGGT CAAGGCGAAG 
ATTGCTGCTC TCTACCTCCC GCTAGTGGGC ATCATTATTG ACTCCATCAA TTACCTGGAC 
TTCACAGTTT CTGACTCTCG TAGTGGTAAA GGCAAGTCTG GTGGGCCTGA GGAAGACCCT 
GACAACATTG CCACCATCAA TCAGTCTGTT GCAATGGCAA TCGCTGGAAA TCCTTTCAAC 
ACCTTAACCA GGAACGCACT GGTGTCTATG GCCTCTGTGA GTGTGAAAAC CACCAACATG 
TTGACGGCGG AGACCAGTCG CAATCTGCTG ATGTGTTTCC TGTGGATCAT CAAGAACGCA 
GACCAGAATC TGATCCAGCG CTGGACTGTG GACATGCCTT CATCTCAGCT GAGTCGACTG 
CTGGAGCTCC TCGCCATCTG CACCTCCTGC TTCGAGTACA GGGGGAAGCA GAGCTCTGAT 
AAGGTGAGCA CTCAGGCACT CCAGAAGTCT CAGCAGGCCA AGGCGCGTCT GGAGGAGGCT 
CTGCTGGGAG GAATCGGAGC CAGAGGAGAA ATGATGAAAC GGGTGGGAGT GCAAGGTAAC 
GACCGGACAC TGGGTCTGAG AGAGAACCTA CGCTGGAGGA AAGACCTTAC ACAGTGGCGC 
CAGACAAACG ACAGGCAGGA CAAATCCAAA GCAGAGCTGG ATCAAGAGGC CATAATCAGT 
GGGAATCTGG CGACTGAGAC CAACCTCATA GTGCTGGACC TGCTGGAGAT GATCGTACAG 
GCAGTTCCTC TGGCCGACTG TAAGGATAAT GTGGTTGGCG GAGTGTTGAA GGTTTTGCTG 
CACTCGCTCA CCTGTAATCA GAGCACCACC TACTTGTCGC ACTGTTTCAG CACTCTGAGG 
GCTCTCATTG TCAAGTTTGG CGACCTGTTG TTCGAGGAGG AGGCCGAGCA GTGTGCAGAC 
CTGTGCCAGA AGGTTCTTCA TTACTGCAGC AGTTGTGTGG ACGGCAACAG GAGTCAGGCT 
TGCGCTACGC TCTACCTGAT CATGAGATAC AGCTACAGCT CAGCTAGCAA CTTCTCCAGA 
GTCAAGATGC AGGTGACAAT GTCTTTGGCC TCACTGGTCG GCAAGTCTTC AGACATCCAC 
GAAGAGTATC TACGCCGCTC TTTCAGAACT ATCCTGGCAT ATGCTGAAGA GGACACCGAA 
ATGCAGTCTA CACAGCTGCC CTCACAGGTA GATGAACTCC TGAGGAACCT AAACAGTATC 
CTCTCAGATA CAGTCAAGAT GAAGGAGTTC CAGAAAGATC CCGAGATGCT TATGGACCTC 
ATGTATAGGA TCGCGAAGGG CTACCAGACT TCTCCAGACC TGCGTCTAAC CTGGCTTCAA 
AACATGGCAG AGAAACACAA CGGCAGGAAG TGCTTCACTG AGTCGGCCAT GTGTCTGGTT 
CACGCCGCAG CCCTGGTGGC TGAATATCTC AGCATGCTGG AGGATCACAA ATACTTGCCT 
GTGGGCAGCG TCACCTTTCA AAACATCTCT CCTAATGTGC TGGAGGAGTC TGCAGTCTCA 
GACGACATAT TGTCTCCAGA CGAGGATGGG GTTTGCTCAG GACGCTACTT CACCGAAAAT 
GGCCTAGTAG GGCTTCTGGA ACAGGCTGCC GAGCTATTCA GCAATGGCGG GTTGTTTGAG 
GCAGTTAATG AGGTGTACAA GGTAATCGTG CCTATACTGG AGGCCCACAG AGATTTCCGG 
AAACTCGCTT CCACACATGA CAAGCTCCAA AGGGCTTTTG ACAACATCAT TCAGAAGGGT 
CATAAGAGGA TGTTTGGAAC CTACTTCCGC GTGGGATTTT ATGGCTCTAA ATTTGGAGAC 
CTGGATGAGC AAGAATTTAT CTACAAAGAG CCAGGAATCA CCCATTTACC AGAGATATCC 
CACCGGCTCG AGAACTTCTA CAGTCAGTGC TTTGGTGATG GCGTTTTGGA AATGATTAAA 
GATTCCACAC CAGTAGACCG AAACAAGCTG AGTCCCAACA AGGCGTACAT ACAGATCACC 
TTTGTGGAGC CTTACTTTGA CGACTATGAG ATGAAAGACC GCCTCACCAA CTTTGAGAAG 
AACTTCAACC TTCGCCGCTT CATGTACACC ACGCCGTTCA CAAAGAGTGG GCGGCCCAGA 
GGAGAACTCA ACGAGCAATA CAAGCGGAAA ACCATCCTTA CCACTATGCA TGCCTTCCCC 
TACATCAAAA CACGAATTAA CGTCATTCAA AAGGAGGAGT TTGACCTCAC ACCAATCGAG 
GTGGCCATTG AGGACATGCA GAAGAAGACC CGAGAGCTGG CTGAAGCCAC ACACAGGGAG 
AAACCGGATG CTGTGATGCT TCAGATGGTT CTGCAGGGAT CTGTCACAGC CACTGTCAAT 
CAGGGTCCAT TGGAGGTGGC CCAGGTCTTC TTGAATGAAA TCCCAGCAGA CCCCAAGCTC 
TTCCGTCATC ACAACAAACT GCGCTTGTGT TTCAAAGAGT TCATACTGCG GTGTGGAGAG 
GCCATTGAGA AGAACAAGCA ACTCATCACT CCCGATCAGA AGGAGTACCA GCAGGAGATG 
AAGAAAAACT ACAATAAACT GCGAGAGAAC CTGAGGCCAA TGCTGGAGAG AAAGATCCCA 
GAGCTCTACA AGCCCATCAT CAAACCTCGC ATCGAGAACA GAGATTCCTT CAAACGGCAC 
AGCCTCCGTC GAACACAAGA TGAGCATTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_009304069.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302344.1 Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X3, mRNA.

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CloneID	ODa40759
Clone ID Related Accession (Same CDS sequence)	XM_009304069.2 , XM_009304068.2 , XM_021478460.1 , XM_009304069.3
Accession Version	XM_009304069.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302344.1
CDS	122..6187
Translation	MSQLRSGDAEVMQELGEFPDDDLDVELVERECRTIRPTIAEEGVELDSHVRDCVQSYTQPWLVVSRRSQGYGWISYSDRTDSNKILQRQTFESDVQPDKQEQLDKSPSLTALCDDSARTTLTSSDFNLRALQPDQRVESVLRFSSPEELDRFNQESRQSNRYGELFGLYPPIDEEDAVAIRPIPDCPKEHFGQRILVRCQTVKFEIEIEPLFATMALYDLKEKKKISENFNFDLNSDQMKGFLRTHTPQVDTSTLSRSAIFSISYPSPDIYLVIKIEKVLQQGEIGDCAEPYMVLKESDTTKNRDKLEKLRNQAEGFCQRLGRYRMPLAFATVNIMSVINSTLERETSDTDSINGRGSMDKKGLPRRNSDRFSLMEDNYILSGFKPAVTMCTFIKQEGERLSDEDLFKFLADIKRSSSQRRVKIIPGYLKLEVLPAPDTVPGCLSPELIPVKPVSEKNQRPIKEVLEFPSKEVYVPHNIYRNLLYVYPLRLNLTNRLTSARNITVKIQFMSGEDSSCAMPVIFGKSSGPEFLTEVYTPVTYHNRSPDFYDEIKILLPARLTDKHHLLFTFFHISCQQKQNQTGSVEALIGYSWLPMLNNDRLQTSQQCLPIVLDKLPINYSLHTPEKLPAQVPPVKWLENHKGLFNLELQAVSSVQTQDSHLERFFTLCHALEGKSTFPIRVRDEKIQENMFEHELKLSIISLSSAGLEPLVLFLHQVLDKLFRLIMQPMVIAGQTANLAQIAFESVVSVINSLHNSQELAKDHQGRNCLLATYLYFVFRLPDTQYEIHTSGTGGLMAHPEGRYSTLTRTTATTVGFMLLQSRIRSSSNPDIPAPLSPEDTEVNNILGKVLNHPGSRMSTAANASNSPQSSTGSTRPTNKKLFHEELALQMVVSTGVCRENVYKYAWFFFEVLVKSMSQHVSQLDKKTVSRRNRFSDRFKDDITTIVNVVTAEIGNILVKQQKELEQAEKVNISLAFFLYDLMSLMDRGFVFQLVKNYCNQMAAKSVNMATLISMRLEFLRVLCSHEHYLNLSLFFSSPSSAPASPSPSVSSQTSSSCSFQDNKIAAMFDLSQDFKQRHYLTGLLLTELSTALDIESEGGRVQQKAINATYSLLCAHDLDQRCSRSEVKAKIAALYLPLVGIIIDSINYLDFTVSDSRSGKGKSGGPEEDPDNIATINQSVAMAIAGNPFNTLTRNALVSMASVSVKTTNMLTAETSRNLLMCFLWIIKNADQNLIQRWTVDMPSSQLSRLLELLAICTSCFEYRGKQSSDKVSTQALQKSQQAKARLEEALLGGIGARGEMMKRVGVQGNDRTLGLRENLRWRKDLTQWRQTNDRQDKSKAELDQEAIISGNLATETNLIVLDLLEMIVQAVPLADCKDNVVGGVLKVLLHSLTCNQSTTYLSHCFSTLRALIVKFGDLLFEEEAEQCADLCQKVLHYCSSCVDGNRSQACATLYLIMRYSYSSASNFSRVKMQVTMSLASLVGKSSDIHEEYLRRSFRTILAYAEEDTEMQSTQLPSQVDELLRNLNSILSDTVKMKEFQKDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHNGRKCFTESAMCLVHAAALVAEYLSMLEDHKYLPVGSVTFQNISPNVLEESAVSDDILSPDEDGVCSGRYFTENGLVGLLEQAAELFSNGGLFEAVNEVYKVIVPILEAHRDFRKLASTHDKLQRAFDNIIQKGHKRMFGTYFRVGFYGSKFGDLDEQEFIYKEPGITHLPEISHRLENFYSQCFGDGVLEMIKDSTPVDRNKLSPNKAYIQITFVEPYFDDYEMKDRLTNFEKNFNLRRFMYTTPFTKSGRPRGELNEQYKRKTILTTMHAFPYIKTRINVIQKEEFDLTPIEVAIEDMQKKTRELAEATHREKPDAVMLQMVLQGSVTATVNQGPLEVAQVFLNEIPADPKLFRHHNKLRLCFKEFILRCGEAIEKNKQLITPDQKEYQQEMKKNYNKLRENLRPMLERKIPELYKPIIKPRIENRDSFKRHSLRRTQDEHS

Target ORF information:

RefSeq Version	XM_009304069.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304069.2

ORF Insert Sequence:

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_009304068.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302343.1 Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

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CloneID	ODa40759
Clone ID Related Accession (Same CDS sequence)	XM_009304069.2 , XM_009304068.2 , XM_021478460.1 , XM_009304069.3
Accession Version	XM_009304068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302343.1
CDS	216..6281
Translation	MSQLRSGDAEVMQELGEFPDDDLDVELVERECRTIRPTIAEEGVELDSHVRDCVQSYTQPWLVVSRRSQGYGWISYSDRTDSNKILQRQTFESDVQPDKQEQLDKSPSLTALCDDSARTTLTSSDFNLRALQPDQRVESVLRFSSPEELDRFNQESRQSNRYGELFGLYPPIDEEDAVAIRPIPDCPKEHFGQRILVRCQTVKFEIEIEPLFATMALYDLKEKKKISENFNFDLNSDQMKGFLRTHTPQVDTSTLSRSAIFSISYPSPDIYLVIKIEKVLQQGEIGDCAEPYMVLKESDTTKNRDKLEKLRNQAEGFCQRLGRYRMPLAFATVNIMSVINSTLERETSDTDSINGRGSMDKKGLPRRNSDRFSLMEDNYILSGFKPAVTMCTFIKQEGERLSDEDLFKFLADIKRSSSQRRVKIIPGYLKLEVLPAPDTVPGCLSPELIPVKPVSEKNQRPIKEVLEFPSKEVYVPHNIYRNLLYVYPLRLNLTNRLTSARNITVKIQFMSGEDSSCAMPVIFGKSSGPEFLTEVYTPVTYHNRSPDFYDEIKILLPARLTDKHHLLFTFFHISCQQKQNQTGSVEALIGYSWLPMLNNDRLQTSQQCLPIVLDKLPINYSLHTPEKLPAQVPPVKWLENHKGLFNLELQAVSSVQTQDSHLERFFTLCHALEGKSTFPIRVRDEKIQENMFEHELKLSIISLSSAGLEPLVLFLHQVLDKLFRLIMQPMVIAGQTANLAQIAFESVVSVINSLHNSQELAKDHQGRNCLLATYLYFVFRLPDTQYEIHTSGTGGLMAHPEGRYSTLTRTTATTVGFMLLQSRIRSSSNPDIPAPLSPEDTEVNNILGKVLNHPGSRMSTAANASNSPQSSTGSTRPTNKKLFHEELALQMVVSTGVCRENVYKYAWFFFEVLVKSMSQHVSQLDKKTVSRRNRFSDRFKDDITTIVNVVTAEIGNILVKQQKELEQAEKVNISLAFFLYDLMSLMDRGFVFQLVKNYCNQMAAKSVNMATLISMRLEFLRVLCSHEHYLNLSLFFSSPSSAPASPSPSVSSQTSSSCSFQDNKIAAMFDLSQDFKQRHYLTGLLLTELSTALDIESEGGRVQQKAINATYSLLCAHDLDQRCSRSEVKAKIAALYLPLVGIIIDSINYLDFTVSDSRSGKGKSGGPEEDPDNIATINQSVAMAIAGNPFNTLTRNALVSMASVSVKTTNMLTAETSRNLLMCFLWIIKNADQNLIQRWTVDMPSSQLSRLLELLAICTSCFEYRGKQSSDKVSTQALQKSQQAKARLEEALLGGIGARGEMMKRVGVQGNDRTLGLRENLRWRKDLTQWRQTNDRQDKSKAELDQEAIISGNLATETNLIVLDLLEMIVQAVPLADCKDNVVGGVLKVLLHSLTCNQSTTYLSHCFSTLRALIVKFGDLLFEEEAEQCADLCQKVLHYCSSCVDGNRSQACATLYLIMRYSYSSASNFSRVKMQVTMSLASLVGKSSDIHEEYLRRSFRTILAYAEEDTEMQSTQLPSQVDELLRNLNSILSDTVKMKEFQKDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHNGRKCFTESAMCLVHAAALVAEYLSMLEDHKYLPVGSVTFQNISPNVLEESAVSDDILSPDEDGVCSGRYFTENGLVGLLEQAAELFSNGGLFEAVNEVYKVIVPILEAHRDFRKLASTHDKLQRAFDNIIQKGHKRMFGTYFRVGFYGSKFGDLDEQEFIYKEPGITHLPEISHRLENFYSQCFGDGVLEMIKDSTPVDRNKLSPNKAYIQITFVEPYFDDYEMKDRLTNFEKNFNLRRFMYTTPFTKSGRPRGELNEQYKRKTILTTMHAFPYIKTRINVIQKEEFDLTPIEVAIEDMQKKTRELAEATHREKPDAVMLQMVLQGSVTATVNQGPLEVAQVFLNEIPADPKLFRHHNKLRLCFKEFILRCGEAIEKNKQLITPDQKEYQQEMKKNYNKLRENLRPMLERKIPELYKPIIKPRIENRDSFKRHSLRRTQDEHS

Target ORF information:

RefSeq Version	XM_009304068.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304068.2

ORF Insert Sequence:

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_021478460.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334135.1 Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X3, mRNA.

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CloneID	ODa40759
Clone ID Related Accession (Same CDS sequence)	XM_009304069.2 , XM_009304068.2 , XM_021478460.1 , XM_009304069.3
Accession Version	XM_021478460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334135.1
CDS	290..6355
Translation	MSQLRSGDAEVMQELGEFPDDDLDVELVERECRTIRPTIAEEGVELDSHVRDCVQSYTQPWLVVSRRSQGYGWISYSDRTDSNKILQRQTFESDVQPDKQEQLDKSPSLTALCDDSARTTLTSSDFNLRALQPDQRVESVLRFSSPEELDRFNQESRQSNRYGELFGLYPPIDEEDAVAIRPIPDCPKEHFGQRILVRCQTVKFEIEIEPLFATMALYDLKEKKKISENFNFDLNSDQMKGFLRTHTPQVDTSTLSRSAIFSISYPSPDIYLVIKIEKVLQQGEIGDCAEPYMVLKESDTTKNRDKLEKLRNQAEGFCQRLGRYRMPLAFATVNIMSVINSTLERETSDTDSINGRGSMDKKGLPRRNSDRFSLMEDNYILSGFKPAVTMCTFIKQEGERLSDEDLFKFLADIKRSSSQRRVKIIPGYLKLEVLPAPDTVPGCLSPELIPVKPVSEKNQRPIKEVLEFPSKEVYVPHNIYRNLLYVYPLRLNLTNRLTSARNITVKIQFMSGEDSSCAMPVIFGKSSGPEFLTEVYTPVTYHNRSPDFYDEIKILLPARLTDKHHLLFTFFHISCQQKQNQTGSVEALIGYSWLPMLNNDRLQTSQQCLPIVLDKLPINYSLHTPEKLPAQVPPVKWLENHKGLFNLELQAVSSVQTQDSHLERFFTLCHALEGKSTFPIRVRDEKIQENMFEHELKLSIISLSSAGLEPLVLFLHQVLDKLFRLIMQPMVIAGQTANLAQIAFESVVSVINSLHNSQELAKDHQGRNCLLATYLYFVFRLPDTQYEIHTSGTGGLMAHPEGRYSTLTRTTATTVGFMLLQSRIRSSSNPDIPAPLSPEDTEVNNILGKVLNHPGSRMSTAANASNSPQSSTGSTRPTNKKLFHEELALQMVVSTGVCRENVYKYAWFFFEVLVKSMSQHVSQLDKKTVSRRNRFSDRFKDDITTIVNVVTAEIGNILVKQQKELEQAEKVNISLAFFLYDLMSLMDRGFVFQLVKNYCNQMAAKSVNMATLISMRLEFLRVLCSHEHYLNLSLFFSSPSSAPASPSPSVSSQTSSSCSFQDNKIAAMFDLSQDFKQRHYLTGLLLTELSTALDIESEGGRVQQKAINATYSLLCAHDLDQRCSRSEVKAKIAALYLPLVGIIIDSINYLDFTVSDSRSGKGKSGGPEEDPDNIATINQSVAMAIAGNPFNTLTRNALVSMASVSVKTTNMLTAETSRNLLMCFLWIIKNADQNLIQRWTVDMPSSQLSRLLELLAICTSCFEYRGKQSSDKVSTQALQKSQQAKARLEEALLGGIGARGEMMKRVGVQGNDRTLGLRENLRWRKDLTQWRQTNDRQDKSKAELDQEAIISGNLATETNLIVLDLLEMIVQAVPLADCKDNVVGGVLKVLLHSLTCNQSTTYLSHCFSTLRALIVKFGDLLFEEEAEQCADLCQKVLHYCSSCVDGNRSQACATLYLIMRYSYSSASNFSRVKMQVTMSLASLVGKSSDIHEEYLRRSFRTILAYAEEDTEMQSTQLPSQVDELLRNLNSILSDTVKMKEFQKDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHNGRKCFTESAMCLVHAAALVAEYLSMLEDHKYLPVGSVTFQNISPNVLEESAVSDDILSPDEDGVCSGRYFTENGLVGLLEQAAELFSNGGLFEAVNEVYKVIVPILEAHRDFRKLASTHDKLQRAFDNIIQKGHKRMFGTYFRVGFYGSKFGDLDEQEFIYKEPGITHLPEISHRLENFYSQCFGDGVLEMIKDSTPVDRNKLSPNKAYIQITFVEPYFDDYEMKDRLTNFEKNFNLRRFMYTTPFTKSGRPRGELNEQYKRKTILTTMHAFPYIKTRINVIQKEEFDLTPIEVAIEDMQKKTRELAEATHREKPDAVMLQMVLQGSVTATVNQGPLEVAQVFLNEIPADPKLFRHHNKLRLCFKEFILRCGEAIEKNKQLITPDQKEYQQEMKKNYNKLRENLRPMLERKIPELYKPIIKPRIENRDSFKRHSLRRTQDEHS

Target ORF information:

RefSeq Version	XM_021478460.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478460.1

ORF Insert Sequence:

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dock8 ( XM_009304069.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302344.1 Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

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CloneID	ODa40759
Clone ID Related Accession (Same CDS sequence)	XM_009304069.2 , XM_009304068.2 , XM_021478460.1 , XM_009304069.3
Accession Version	XM_009304069.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dedicator of cytokinesis protein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304069.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302344.1
CDS	108..6173
Translation	MSQLRSGDAEVMQELGEFPDDDLDVELVERECRTIRPTIAEEGVELDSHVRDCVQSYTQPWLVVSRRSQGYGWISYSDRTDSNKILQRQTFESDVQPDKQEQLDKSPSLTALCDDSARTTLTSSDFNLRALQPDQRVESVLRFSSPEELDRFNQESRQSNRYGELFGLYPPIDEEDAVAIRPIPDCPKEHFGQRILVRCQTVKFEIEIEPLFATMALYDLKEKKKISENFNFDLNSDQMKGFLRTHTPQVDTSTLSRSAIFSISYPSPDIYLVIKIEKVLQQGEIGDCAEPYMVLKESDTTKNRDKLEKLRNQAEGFCQRLGRYRMPLAFATVNIMSVINSTLERETSDTDSINGRGSMDKKGLPRRNSDRFSLMEDNYILSGFKPAVTMCTFIKQEGERLSDEDLFKFLADIKRSSSQRRVKIIPGYLKLEVLPAPDTVPGCLSPELIPVKPVSEKNQRPIKEVLEFPSKEVYVPHNIYRNLLYVYPLRLNLTNRLTSARNITVKIQFMSGEDSSCAMPVIFGKSSGPEFLTEVYTPVTYHNRSPDFYDEIKILLPARLTDKHHLLFTFFHISCQQKQNQTGSVEALIGYSWLPMLNNDRLQTSQQCLPIVLDKLPINYSLHTPEKLPAQVPPVKWLENHKGLFNLELQAVSSVQTQDSHLERFFTLCHALEGKSTFPIRVRDEKIQENMFEHELKLSIISLSSAGLEPLVLFLHQVLDKLFRLIMQPMVIAGQTANLAQIAFESVVSVINSLHNSQELAKDHQGRNCLLATYLYFVFRLPDTQYEIHTSGTGGLMAHPEGRYSTLTRTTATTVGFMLLQSRIRSSSNPDIPAPLSPEDTEVNNILGKVLNHPGSRMSTAANASNSPQSSTGSTRPTNKKLFHEELALQMVVSTGVCRENVYKYAWFFFEVLVKSMSQHVSQLDKKTVSRRNRFSDRFKDDITTIVNVVTAEIGNILVKQQKELEQAEKVNISLAFFLYDLMSLMDRGFVFQLVKNYCNQMAAKSVNMATLISMRLEFLRVLCSHEHYLNLSLFFSSPSSAPASPSPSVSSQTSSSCSFQDNKIAAMFDLSQDFKQRHYLTGLLLTELSTALDIESEGGRVQQKAINATYSLLCAHDLDQRCSRSEVKAKIAALYLPLVGIIIDSINYLDFTVSDSRSGKGKSGGPEEDPDNIATINQSVAMAIAGNPFNTLTRNALVSMASVSVKTTNMLTAETSRNLLMCFLWIIKNADQNLIQRWTVDMPSSQLSRLLELLAICTSCFEYRGKQSSDKVSTQALQKSQQAKARLEEALLGGIGARGEMMKRVGVQGNDRTLGLRENLRWRKDLTQWRQTNDRQDKSKAELDQEAIISGNLATETNLIVLDLLEMIVQAVPLADCKDNVVGGVLKVLLHSLTCNQSTTYLSHCFSTLRALIVKFGDLLFEEEAEQCADLCQKVLHYCSSCVDGNRSQACATLYLIMRYSYSSASNFSRVKMQVTMSLASLVGKSSDIHEEYLRRSFRTILAYAEEDTEMQSTQLPSQVDELLRNLNSILSDTVKMKEFQKDPEMLMDLMYRIAKGYQTSPDLRLTWLQNMAEKHNGRKCFTESAMCLVHAAALVAEYLSMLEDHKYLPVGSVTFQNISPNVLEESAVSDDILSPDEDGVCSGRYFTENGLVGLLEQAAELFSNGGLFEAVNEVYKVIVPILEAHRDFRKLASTHDKLQRAFDNIIQKGHKRMFGTYFRVGFYGSKFGDLDEQEFIYKEPGITHLPEISHRLENFYSQCFGDGVLEMIKDSTPVDRNKLSPNKAYIQITFVEPYFDDYEMKDRLTNFEKNFNLRRFMYTTPFTKSGRPRGELNEQYKRKTILTTMHAFPYIKTRINVIQKEEFDLTPIEVAIEDMQKKTRELAEATHREKPDAVMLQMVLQGSVTATVNQGPLEVAQVFLNEIPADPKLFRHHNKLRLCFKEFILRCGEAIEKNKQLITPDQKEYQQEMKKNYNKLRENLRPMLERKIPELYKPIIKPRIENRDSFKRHSLRRTQDEHS

Target ORF information:

RefSeq Version	XM_009304069.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dedicator of cytokinesis 8 (dock8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304069.3

ORF Insert Sequence:

ATGTCTCAGC TGCGCAGTGG CGATGCCGAG GTCATGCAGG AGTTGGGTGA GTTTCCTGAT 
GACGACCTGG ATGTTGAGCT TGTGGAGAGG GAATGCAGGA CCATCCGCCC CACTATTGCT 
GAGGAAGGAG TGGAGCTGGA TTCCCATGTG AGGGATTGTG TTCAGAGTTA CACGCAGCCT 
TGGCTGGTGG TGAGCAGAAG AAGTCAGGGT TATGGATGGA TTTCATACTC TGATAGAACA 
GACTCTAACA AAATCTTACA GAGGCAGACA TTTGAGTCTG ACGTTCAGCC AGACAAACAG 
GAGCAACTAG ATAAGTCGCC GTCGCTGACA GCACTGTGTG ATGATTCAGC TCGGACCACC 
CTCACAAGCT CAGATTTTAA CCTGAGGGCC CTTCAGCCAG ACCAGCGAGT GGAAAGTGTC 
CTGCGTTTCA GCAGCCCAGA GGAATTGGAC CGCTTCAACC AGGAGTCCAG ACAGAGCAAC 
CGCTATGGCG AACTCTTTGG CCTCTACCCG CCTATTGATG AGGAGGATGC TGTTGCAATT 
CGGCCTATTC CTGACTGTCC AAAGGAGCAT TTTGGCCAAA GGATATTAGT GCGATGCCAG 
ACCGTCAAAT TTGAGATTGA AATCGAGCCT CTCTTTGCAA CAATGGCCCT CTATGACCTG 
AAGGAGAAGA AGAAGATTTC AGAGAACTTC AACTTTGATC TGAACTCAGA TCAGATGAAG 
GGTTTCCTGC GGACCCACAC TCCTCAAGTT GACACTTCCA CTTTGTCCAG ATCTGCAATT 
TTTTCCATCT CCTACCCCTC TCCAGACATC TATCTGGTCA TTAAGATTGA GAAGGTTCTT 
CAGCAGGGAG AGATTGGGGA TTGTGCTGAA CCATACATGG TACTGAAGGA GAGTGACACG 
ACTAAGAATA GAGACAAGCT GGAGAAGTTG CGGAATCAGG CGGAGGGGTT TTGTCAAAGA 
CTGGGTCGCT ACAGGATGCC ATTAGCCTTT GCTACGGTCA ACATCATGAG TGTCATCAAC 
TCCACACTGG AACGAGAGAC AAGTGACACA GACAGTATTA ATGGCAGGGG GAGTATGGAC 
AAGAAGGGAT TGCCGAGACG TAACTCTGAC AGATTCAGCT TGATGGAGGA CAACTACATC 
CTGTCAGGCT TCAAACCAGC TGTCACTATG TGCACATTCA TCAAACAGGA AGGAGAACGT 
CTGAGTGACG AAGATCTGTT TAAGTTCCTG GCGGATATAA AGAGGTCATC CAGTCAGAGA 
CGTGTTAAAA TCATACCTGG TTATCTGAAA CTGGAAGTGC TTCCAGCGCC GGACACGGTT 
CCTGGGTGTT TGTCTCCTGA ACTGATTCCA GTGAAGCCTG TGTCTGAGAA AAACCAACGT 
CCGATTAAAG AAGTGCTCGA GTTCCCTTCG AAGGAAGTTT ATGTGCCTCA CAATATATAC 
CGGAATCTGC TGTATGTATA TCCGCTGAGG TTAAATCTTA CTAACCGTCT GACTTCAGCC 
AGAAACATTA CAGTAAAAAT CCAGTTCATG AGCGGTGAGG ACTCAAGTTG TGCCATGCCT 
GTAATCTTTG GAAAATCAAG TGGCCCGGAG TTCCTTACGG AGGTCTACAC TCCAGTCACT 
TACCACAACA GGTCTCCTGA TTTCTATGAC GAGATAAAGA TCCTCCTGCC AGCCCGTTTA 
ACAGATAAAC ACCACTTGCT GTTCACCTTC TTCCATATCA GCTGCCAGCA GAAACAAAAC 
CAGACTGGAA GTGTGGAGGC TCTCATTGGA TATTCATGGT TACCCATGCT GAACAATGAC 
CGGCTGCAGA CCAGCCAGCA GTGTCTGCCA ATCGTTCTGG ACAAGCTTCC TATCAACTAT 
TCCCTACATA CCCCAGAGAA ACTGCCAGCT CAGGTACCTC CAGTGAAGTG GCTGGAGAAT 
CATAAAGGAC TGTTTAACCT TGAGCTACAA GCTGTGTCCT CAGTTCAAAC TCAGGACAGT 
CACCTGGAGC GTTTCTTCAC ATTATGTCAT GCTTTGGAGG GCAAATCCAC ATTCCCCATC 
CGAGTGCGAG ATGAGAAGAT TCAGGAAAAC ATGTTTGAGC ATGAGCTGAA GCTCAGCATC 
ATTTCACTGT CCTCCGCCGG CCTGGAGCCG CTGGTGCTTT TTCTCCATCA GGTCCTCGAC 
AAGCTCTTCC GTCTCATCAT GCAGCCCATG GTCATCGCTG GACAGACTGC AAATCTGGCC 
CAAATTGCGT TTGAGTCAGT GGTGTCGGTC ATCAACAGTC TTCACAACAG TCAGGAGCTG 
GCAAAAGACC ATCAGGGCAG AAACTGTCTC CTCGCAACAT ACCTGTACTT TGTGTTTCGC 
TTGCCAGATA CTCAATATGA AATCCACACC TCAGGTACAG GAGGTCTGAT GGCTCATCCA 
GAGGGTCGAT ACAGCACTTT AACTCGCACA ACGGCCACCA CTGTTGGCTT CATGCTGCTG 
CAGTCACGAA TCCGTTCCAG CAGCAACCCT GATATACCAG CACCACTGAG CCCAGAGGAC 
ACAGAGGTCA ATAACATTCT AGGAAAGGTT TTAAATCATC CAGGCAGTCG CATGTCAACA 
GCTGCAAATG CCTCCAATTC TCCGCAGAGC TCCACGGGAA GCACGCGGCC CACCAACAAA 
AAGTTGTTCC ACGAGGAGCT GGCTCTTCAG ATGGTGGTCA GCACCGGAGT CTGCAGAGAA 
AATGTCTACA AATACGCCTG GTTCTTCTTT GAAGTTCTGG TGAAGAGCAT GAGTCAGCAC 
GTGTCTCAGC TGGATAAGAA GACGGTCTCT CGCAGGAATC GATTTTCCGA CCGTTTCAAA 
GATGACATCA CCACCATTGT CAACGTGGTG ACCGCTGAGA TCGGGAACAT TTTAGTAAAG 
CAGCAAAAGG AGCTGGAGCA GGCAGAGAAG GTTAACATCA GCCTGGCTTT CTTCCTGTAT 
GACCTGATGT CTCTGATGGA TCGAGGCTTT GTGTTTCAGC TGGTGAAGAA TTACTGCAAC 
CAGATGGCAG CAAAGAGTGT GAACATGGCG ACTTTGATAA GTATGAGGCT GGAGTTTTTG 
AGGGTCTTGT GCAGTCATGA GCACTACTTG AACCTCAGTC TCTTCTTCAG CAGCCCTTCA 
TCTGCCCCTG CCTCCCCATC ACCCTCGGTG TCCTCACAGA CGTCCAGCTC GTGCAGTTTT 
CAGGACAACA AGATCGCAGC CATGTTTGAT TTGTCGCAGG ACTTTAAGCA GCGCCATTAC 
TTGACCGGAC TCTTGCTAAC TGAACTCAGC ACTGCACTGG ACATAGAGTC CGAGGGGGGC 
AGAGTTCAAC AAAAAGCCAT CAATGCCACA TACAGTCTAT TATGTGCTCA TGATCTGGAC 
CAGCGCTGCT CAAGGTCAGA GGTCAAGGCG AAGATTGCTG CTCTCTACCT CCCGCTAGTG 
GGCATCATTA TTGACTCCAT CAATTACCTG GACTTCACAG TTTCTGACTC TCGTAGTGGT 
AAAGGCAAGT CTGGTGGGCC TGAGGAAGAC CCTGACAACA TTGCCACCAT CAATCAGTCT 
GTTGCAATGG CAATCGCTGG AAATCCTTTC AACACCTTAA CCAGGAACGC ACTGGTGTCT 
ATGGCCTCTG TGAGTGTGAA AACCACCAAC ATGTTGACGG CGGAGACCAG TCGCAATCTG 
CTGATGTGTT TCCTGTGGAT CATCAAGAAC GCAGACCAGA ATCTGATCCA GCGCTGGACT 
GTGGACATGC CTTCATCTCA GCTGAGTCGA CTGCTGGAGC TCCTCGCCAT CTGCACCTCC 
TGCTTCGAGT ACAGGGGGAA GCAGAGCTCT GATAAGGTGA GCACTCAGGC ACTCCAGAAG 
TCTCAGCAGG CCAAGGCGCG TCTGGAGGAG GCTCTGCTGG GAGGAATCGG AGCCAGAGGA 
GAAATGATGA AACGGGTGGG AGTGCAAGGT AACGACCGGA CACTGGGTCT GAGAGAGAAC 
CTACGCTGGA GGAAAGACCT TACACAGTGG CGCCAGACAA ACGACAGGCA GGACAAATCC 
AAAGCAGAGC TGGATCAAGA GGCCATAATC AGTGGGAATC TGGCGACTGA GACCAACCTC 
ATAGTGCTGG ACCTGCTGGA GATGATCGTA CAGGCAGTTC CTCTGGCCGA CTGTAAGGAT 
AATGTGGTTG GCGGAGTGTT GAAGGTTTTG CTGCACTCGC TCACCTGTAA TCAGAGCACC 
ACCTACTTGT CGCACTGTTT CAGCACTCTG AGGGCTCTCA TTGTCAAGTT TGGCGACCTG 
TTGTTCGAGG AGGAGGCCGA GCAGTGTGCA GACCTGTGCC AGAAGGTTCT TCATTACTGC 
AGCAGTTGTG TGGACGGCAA CAGGAGTCAG GCTTGCGCTA CGCTCTACCT GATCATGAGA 
TACAGCTACA GCTCAGCTAG CAACTTCTCC AGAGTCAAGA TGCAGGTGAC AATGTCTTTG 
GCCTCACTGG TCGGCAAGTC TTCAGACATC CACGAAGAGT ATCTACGCCG CTCTTTCAGA 
ACTATCCTGG CATATGCTGA AGAGGACACC GAAATGCAGT CTACACAGCT GCCCTCACAG 
GTAGATGAAC TCCTGAGGAA CCTAAACAGT ATCCTCTCAG ATACAGTCAA GATGAAGGAG 
TTCCAGAAAG ATCCCGAGAT GCTTATGGAC CTCATGTATA GGATCGCGAA GGGCTACCAG 
ACTTCTCCAG ACCTGCGTCT AACCTGGCTT CAAAACATGG CAGAGAAACA CAACGGCAGG 
AAGTGCTTCA CTGAGTCGGC CATGTGTCTG GTTCACGCCG CAGCCCTGGT GGCTGAATAT 
CTCAGCATGC TGGAGGATCA CAAATACTTG CCTGTGGGCA GCGTCACCTT TCAAAACATC 
TCTCCTAATG TGCTGGAGGA GTCTGCAGTC TCAGACGACA TATTGTCTCC AGACGAGGAT 
GGGGTTTGCT CAGGACGCTA CTTCACCGAA AATGGCCTAG TAGGGCTTCT GGAACAGGCT 
GCCGAGCTAT TCAGCAATGG CGGGTTGTTT GAGGCAGTTA ATGAGGTGTA CAAGGTAATC 
GTGCCTATAC TGGAGGCCCA CAGAGATTTC CGGAAACTCG CTTCCACACA TGACAAGCTC 
CAAAGGGCTT TTGACAACAT CATTCAGAAG GGTCATAAGA GGATGTTTGG AACCTACTTC 
CGCGTGGGAT TTTATGGCTC TAAATTTGGA GACCTGGATG AGCAAGAATT TATCTACAAA 
GAGCCAGGAA TCACCCATTT ACCAGAGATA TCCCACCGGC TCGAGAACTT CTACAGTCAG 
TGCTTTGGTG ATGGCGTTTT GGAAATGATT AAAGATTCCA CACCAGTAGA CCGAAACAAG 
CTGAGTCCCA ACAAGGCGTA CATACAGATC ACCTTTGTGG AGCCTTACTT TGACGACTAT 
GAGATGAAAG ACCGCCTCAC CAACTTTGAG AAGAACTTCA ACCTTCGCCG CTTCATGTAC 
ACCACGCCGT TCACAAAGAG TGGGCGGCCC AGAGGAGAAC TCAACGAGCA ATACAAGCGG 
AAAACCATCC TTACCACTAT GCATGCCTTC CCCTACATCA AAACACGAAT TAACGTCATT 
CAAAAGGAGG AGTTTGACCT CACACCAATC GAGGTGGCCA TTGAGGACAT GCAGAAGAAG 
ACCCGAGAGC TGGCTGAAGC CACACACAGG GAGAAACCGG ATGCTGTGAT GCTTCAGATG 
GTTCTGCAGG GATCTGTCAC AGCCACTGTC AATCAGGGTC CATTGGAGGT GGCCCAGGTC 
TTCTTGAATG AAATCCCAGC AGACCCCAAG CTCTTCCGTC ATCACAACAA ACTGCGCTTG 
TGTTTCAAAG AGTTCATACT GCGGTGTGGA GAGGCCATTG AGAAGAACAA GCAACTCATC 
ACTCCCGATC AGAAGGAGTA CCAGCAGGAG ATGAAGAAAA ACTACAATAA ACTGCGAGAG 
AACCTGAGGC CAATGCTGGA GAGAAAGATC CCAGAGCTCT ACAAGCCCAT CATCAAACCT 
CGCATCGAGA ACAGAGATTC CTTCAAACGG CACAGCCTCC GTCGAACACA AGATGAGCAT 
TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dock8 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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