ORF » Species Summary » Danio rerio » bub3 cDNA ORF clone

bub3 cDNA ORF clone, Danio rerio(zebrafish)

  • Gene

  • Clones

  • gRNAs

The following bub3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bub3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03072 NM_205709.1
Latest version!		 Danio rerio BUB3 mitotic checkpoint protein (bub3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
ODa18314 XM_005169505.4
Latest version!		 Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
ODa54446 XM_017358443.1
Latest version!		 Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
View Old Accession Versions >>
ODa18314 XM_005169505.3 Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    bub3 ( NM_205709.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_991272.1 Danio rerio BUB3 mitotic checkpoint protein (bub3), mRNA.
    Price & Availability↑
    CloneID ODa03072
    Clone ID Related Accession (Same CDS sequence) NM_205709.1
    Accession Version NM_205709.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 981bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product mitotic checkpoint protein BUB3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY391411.1. ##Evidence-Data-START## Transcript exon combination :: AY391411.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476732, SAMEA4476735 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACCGGAG CGAATGAGTT TAAGCTGGCC CAGGGCCCGG AGGACAGCGT CTCGGCAGTG 
    AAGTTCAGCC CGTCCTCCTC TCAGTTCCTG CTGGTGTCCT CTTGGGACGG CTCCGTGCGG 
    CTCTACGACG CCAGCGCCAA CAGCATGAGG ATGAAGTACC AACACCTGGC GCCCGTGCTG 
    GACTGCGCTT TCTCTGACCC AACCCATGCA TGGAGCGGTG GTCTGGACAG TCAGCTGAAG 
    ACTCATGACC TGAACACTGA TCAGGACACT ATCGTGGGCA CACACGACGC CCCGATCCGC 
    TGTGTGGAGT TCTGCCCGGA GGTCAATGTT CTGGTGACGG GCAGCTGGGA TCAGTCGGTG 
    CGGCTCTGGG ACCCGCGAAC ACCCTGCAAC GCCGGCACAT TCACACAGCC TGAGAAGGTG 
    TACACTCTCT CTGTGGCGGG GGATCGTCTG ATCGTGGGGA CGGCGGGTCG GCGGGTGCTG 
    GTGTGGGATC TGAGGAACAT GGGATACGTG CAGCAGCGCC GCGAGTCCAG CCTCAAGTAC 
    CAGACGCGCT GCATACGGGC CTTCCCCAAC AAACAGGGCT ATGTGCTGAG CTCCATCGAG 
    GGCCGTGTGG CTGTGGAGTA TCTGGACCCC AGTCTGGAGG TGCAGAAGAA GAAATACGCC 
    TTCAAGTGCC ACAGACTGAA GGAGAACGGC ATCGAGCAGG TGTATCCGGT CAACGCCATC 
    TCCTTCCACA GCGTGCACAA CACCTTCGCC ACCGGTGGTT CAGACGGTTT CGTCAACATC 
    TGGGACCCGT TCAATAAGAA GCGTCTGTGT CAGTTCCACC GGTACCCCAG CAGCATCGCC 
    TCTCTGTCCT TCAGCACGGA CGGCTCTCTG CTGGCCATCG CCTCCTCATA CATGCAGGAG 
    CTGGGGGACG TCTCACACCC CGCTGACGCT GTGTTCATCC GCCAGGTCAC CGACGCAGAG 
    ACCAAGCCCA AATCGACGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_991272.1
    CDS2..982
    Translation

    Target ORF information:

    RefSeq Version NM_205709.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio BUB3 mitotic checkpoint protein (bub3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_205709.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACCGGAG CGAATGAGTT TAAGCTGGCC CAGGGCCCGG AGGACAGCGT CTCGGCAGTG 
    AAGTTCAGCC CGTCCTCCTC TCAGTTCCTG CTGGTGTCCT CTTGGGACGG CTCCGTGCGG 
    CTCTACGACG CCAGCGCCAA CAGCATGAGG ATGAAGTACC AACACCTGGC GCCCGTGCTG 
    GACTGCGCTT TCTCTGACCC AACCCATGCA TGGAGCGGTG GTCTGGACAG TCAGCTGAAG 
    ACTCATGACC TGAACACTGA TCAGGACACT ATCGTGGGCA CACACGACGC CCCGATCCGC 
    TGTGTGGAGT TCTGCCCGGA GGTCAATGTT CTGGTGACGG GCAGCTGGGA TCAGTCGGTG 
    CGGCTCTGGG ACCCGCGAAC ACCCTGCAAC GCCGGCACAT TCACACAGCC TGAGAAGGTG 
    TACACTCTCT CTGTGGCGGG GGATCGTCTG ATCGTGGGGA CGGCGGGTCG GCGGGTGCTG 
    GTGTGGGATC TGAGGAACAT GGGATACGTG CAGCAGCGCC GCGAGTCCAG CCTCAAGTAC 
    CAGACGCGCT GCATACGGGC CTTCCCCAAC AAACAGGGCT ATGTGCTGAG CTCCATCGAG 
    GGCCGTGTGG CTGTGGAGTA TCTGGACCCC AGTCTGGAGG TGCAGAAGAA GAAATACGCC 
    TTCAAGTGCC ACAGACTGAA GGAGAACGGC ATCGAGCAGG TGTATCCGGT CAACGCCATC 
    TCCTTCCACA GCGTGCACAA CACCTTCGCC ACCGGTGGTT CAGACGGTTT CGTCAACATC 
    TGGGACCCGT TCAATAAGAA GCGTCTGTGT CAGTTCCACC GGTACCCCAG CAGCATCGCC 
    TCTCTGTCCT TCAGCACGGA CGGCTCTCTG CTGGCCATCG CCTCCTCATA CATGCAGGAG 
    CTGGGGGACG TCTCACACCC CGCTGACGCT GTGTTCATCC GCCAGGTCAC CGACGCAGAG 
    ACCAAGCCCA AATCGACGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    bub3 ( XM_005169505.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169562.1 Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa18314
    Clone ID Related Accession (Same CDS sequence) XM_005169505.3 , XM_005169505.4
    Accession Version XM_005169505.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitotic checkpoint protein BUB3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA GAGCAGCAGC AGAGAACAGC GGCACGATGA CCGGAGCGAA TGAGTTTAAG 
    CTGGCCCAGG GCCCGGAGGA CAGCGTCTCG GCAGTGAAGT TCAGCCCGTC CTCCTCTCAG 
    TTCCTGCTGG TGTCCTCTTG GGACGGCTCC GTGCGGCTCT ACGACGCCAG CGCCAACAGC 
    ATGAGGATGA AGTACCAACA CCTGGCGCCC GTGCTGGACT GCGCTTTCTC TGACCCAACC 
    CATGCGTGGA GCGGTGGTCT GGACAGTCAG CTGAAGACTC ATGACCTGAA CACTGATCAG 
    GACACTATCG TGGGCACACA CGACGCCCCG ATCCGCTGTG TGGAGTTCTG CCCGGAGGTC 
    AATGTTCTGG TGACGGGCAG CTGGGATCAG TCGGTGCGGC TCTGGGACCC GCGAACACCC 
    TGCAACGCCG GCACATTCAC ACAGCCTGAG AAGGTGTACA CTCTCTCTGT GGCGGGGGAT 
    CGTCTGATCG TGGGGACGGC GGGTCGCCGG GTGCTGGTGT GGGATCTGAG GAACATGGGA 
    TACGTGCAGC AGCGCCGCGA GTCCAGCCTC AAGTACCAGA CGCGCTGCAT ACGGGCCTTC 
    CCCAACAAAC AGGGCTATGT GCTGAGCTCC ATCGAGGGCC GTGTGGCTGT GGAGTATCTG 
    GACCCCAGTC TGGAGGTGCA GAAGAAGAAA TACGCCTTCA AGTGCCACAG ACTGAAGGAG 
    AACGGCATCG AGCAGGTGTA TCCGGTCAAC GCCATCTCCT TCCACAGCGT GCACAACACC 
    TTTGCCACCG GTGGTTCAGA CGGTTTCGTC AACATCTGGG ACCCGTTCAA TAAGAAGCGT 
    CTGTGTCAGT TCCACCGGTA CCCCAGCAGC ATCGCCTCTC TGTCCTTCAG CACGGACGGC 
    TCTCTGCTGG CCATCGCCTC CTCATACATG CAGGAGCTGG GGGACGTCTC ACACCCCGCT 
    GACGCTGTGT TCATCCGCCA GGTCACCGAT GCAGAGACCA AGCCCAAATC GACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169562.1
    CDS73..1089
    Translation

    Target ORF information:

    RefSeq Version XM_005169505.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005169505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA GAGCAGCAGC AGAGAACAGC GGCACGATGA CCGGAGCGAA TGAGTTTAAG 
    CTGGCCCAGG GCCCGGAGGA CAGCGTCTCG GCAGTGAAGT TCAGCCCGTC CTCCTCTCAG 
    TTCCTGCTGG TGTCCTCTTG GGACGGCTCC GTGCGGCTCT ACGACGCCAG CGCCAACAGC 
    ATGAGGATGA AGTACCAACA CCTGGCGCCC GTGCTGGACT GCGCTTTCTC TGACCCAACC 
    CATGCGTGGA GCGGTGGTCT GGACAGTCAG CTGAAGACTC ATGACCTGAA CACTGATCAG 
    GACACTATCG TGGGCACACA CGACGCCCCG ATCCGCTGTG TGGAGTTCTG CCCGGAGGTC 
    AATGTTCTGG TGACGGGCAG CTGGGATCAG TCGGTGCGGC TCTGGGACCC GCGAACACCC 
    TGCAACGCCG GCACATTCAC ACAGCCTGAG AAGGTGTACA CTCTCTCTGT GGCGGGGGAT 
    CGTCTGATCG TGGGGACGGC GGGTCGCCGG GTGCTGGTGT GGGATCTGAG GAACATGGGA 
    TACGTGCAGC AGCGCCGCGA GTCCAGCCTC AAGTACCAGA CGCGCTGCAT ACGGGCCTTC 
    CCCAACAAAC AGGGCTATGT GCTGAGCTCC ATCGAGGGCC GTGTGGCTGT GGAGTATCTG 
    GACCCCAGTC TGGAGGTGCA GAAGAAGAAA TACGCCTTCA AGTGCCACAG ACTGAAGGAG 
    AACGGCATCG AGCAGGTGTA TCCGGTCAAC GCCATCTCCT TCCACAGCGT GCACAACACC 
    TTTGCCACCG GTGGTTCAGA CGGTTTCGTC AACATCTGGG ACCCGTTCAA TAAGAAGCGT 
    CTGTGTCAGT TCCACCGGTA CCCCAGCAGC ATCGCCTCTC TGTCCTTCAG CACGGACGGC 
    TCTCTGCTGG CCATCGCCTC CTCATACATG CAGGAGCTGG GGGACGTCTC ACACCCCGCT 
    GACGCTGTGT TCATCCGCCA GGTCACCGAT GCAGAGACCA AGCCCAAATC GACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    bub3 ( XM_005169505.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005169562.1 Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa18314
    Clone ID Related Accession (Same CDS sequence) XM_005169505.3 , XM_005169505.4
    Accession Version XM_005169505.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product mitotic checkpoint protein BUB3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA GAGCAGCAGC AGAGAACAGC GGCACGATGA CCGGAGCGAA TGAGTTTAAG 
    CTGGCCCAGG GCCCGGAGGA CAGCGTCTCG GCAGTGAAGT TCAGCCCGTC CTCCTCTCAG 
    TTCCTGCTGG TGTCCTCTTG GGACGGCTCC GTGCGGCTCT ACGACGCCAG CGCCAACAGC 
    ATGAGGATGA AGTACCAACA CCTGGCGCCC GTGCTGGACT GCGCTTTCTC TGACCCAACC 
    CATGCGTGGA GCGGTGGTCT GGACAGTCAG CTGAAGACTC ATGACCTGAA CACTGATCAG 
    GACACTATCG TGGGCACACA CGACGCCCCG ATCCGCTGTG TGGAGTTCTG CCCGGAGGTC 
    AATGTTCTGG TGACGGGCAG CTGGGATCAG TCGGTGCGGC TCTGGGACCC GCGAACACCC 
    TGCAACGCCG GCACATTCAC ACAGCCTGAG AAGGTGTACA CTCTCTCTGT GGCGGGGGAT 
    CGTCTGATCG TGGGGACGGC GGGTCGCCGG GTGCTGGTGT GGGATCTGAG GAACATGGGA 
    TACGTGCAGC AGCGCCGCGA GTCCAGCCTC AAGTACCAGA CGCGCTGCAT ACGGGCCTTC 
    CCCAACAAAC AGGGCTATGT GCTGAGCTCC ATCGAGGGCC GTGTGGCTGT GGAGTATCTG 
    GACCCCAGTC TGGAGGTGCA GAAGAAGAAA TACGCCTTCA AGTGCCACAG ACTGAAGGAG 
    AACGGCATCG AGCAGGTGTA TCCGGTCAAC GCCATCTCCT TCCACAGCGT GCACAACACC 
    TTTGCCACCG GTGGTTCAGA CGGTTTCGTC AACATCTGGG ACCCGTTCAA TAAGAAGCGT 
    CTGTGTCAGT TCCACCGGTA CCCCAGCAGC ATCGCCTCTC TGTCCTTCAG CACGGACGGC 
    TCTCTGCTGG CCATCGCCTC CTCATACATG CAGGAGCTGG GGGACGTCTC ACACCCCGCT 
    GACGCTGTGT TCATCCGCCA GGTCACCGAT GCAGAGACCA AGCCCAAATC GACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005169562.1
    CDS114..1130
    Translation

    Target ORF information:

    RefSeq Version XM_005169505.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005169505.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA GAGCAGCAGC AGAGAACAGC GGCACGATGA CCGGAGCGAA TGAGTTTAAG 
    CTGGCCCAGG GCCCGGAGGA CAGCGTCTCG GCAGTGAAGT TCAGCCCGTC CTCCTCTCAG 
    TTCCTGCTGG TGTCCTCTTG GGACGGCTCC GTGCGGCTCT ACGACGCCAG CGCCAACAGC 
    ATGAGGATGA AGTACCAACA CCTGGCGCCC GTGCTGGACT GCGCTTTCTC TGACCCAACC 
    CATGCGTGGA GCGGTGGTCT GGACAGTCAG CTGAAGACTC ATGACCTGAA CACTGATCAG 
    GACACTATCG TGGGCACACA CGACGCCCCG ATCCGCTGTG TGGAGTTCTG CCCGGAGGTC 
    AATGTTCTGG TGACGGGCAG CTGGGATCAG TCGGTGCGGC TCTGGGACCC GCGAACACCC 
    TGCAACGCCG GCACATTCAC ACAGCCTGAG AAGGTGTACA CTCTCTCTGT GGCGGGGGAT 
    CGTCTGATCG TGGGGACGGC GGGTCGCCGG GTGCTGGTGT GGGATCTGAG GAACATGGGA 
    TACGTGCAGC AGCGCCGCGA GTCCAGCCTC AAGTACCAGA CGCGCTGCAT ACGGGCCTTC 
    CCCAACAAAC AGGGCTATGT GCTGAGCTCC ATCGAGGGCC GTGTGGCTGT GGAGTATCTG 
    GACCCCAGTC TGGAGGTGCA GAAGAAGAAA TACGCCTTCA AGTGCCACAG ACTGAAGGAG 
    AACGGCATCG AGCAGGTGTA TCCGGTCAAC GCCATCTCCT TCCACAGCGT GCACAACACC 
    TTTGCCACCG GTGGTTCAGA CGGTTTCGTC AACATCTGGG ACCCGTTCAA TAAGAAGCGT 
    CTGTGTCAGT TCCACCGGTA CCCCAGCAGC ATCGCCTCTC TGTCCTTCAG CACGGACGGC 
    TCTCTGCTGG CCATCGCCTC CTCATACATG CAGGAGCTGG GGGACGTCTC ACACCCCGCT 
    GACGCTGTGT TCATCCGCCA GGTCACCGAT GCAGAGACCA AGCCCAAATC GACGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    bub3 ( XM_017358443.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213932.1 Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID ODa54446
    Clone ID Related Accession (Same CDS sequence) XM_017358443.1
    Accession Version XM_017358443.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product mitotic checkpoint protein BUB3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA GAGCAGCAGC AGAGAACAGC GGCACGATGA CCGGAGCGAA TGAGTTTAAG 
    CTGGCCCAGG GCCCGGAGGA CAGCGTCTCG GCAGTGAAGT TCAGCCCGTC CTCCTCTCAG 
    TTCCTGCTGG TGTCCTCTTG GGACGGCTCC GTGCGGCTCT ACGACGCCAG CGCCAACAGC 
    ATGAGGATGA AGTACCAACA CCTGGCGCCC GTGCTGGACT GCGCTTTCTC TGACCCAACC 
    CATGCGTGGA GCGGTGGTCT GGACAGTCAG CTGAAGACTC ATGACCTGAA CACTGATCAG 
    GACACTATCG TGGGCACACA CGACGCCCCG ATCCGCTGTG TGGAGTTCTG CCCGGAGGTC 
    AATGTTCTGG TGACGGGCAG CTGGGATCAG TCGGTGCGGC TCTGGGACCC GCGAACACCC 
    TGCAACGCCG GCACATTCAC ACAGCCTGAG AAGGTGTACA CTCTCTCTGT GGCGGGGGAT 
    CGTCTGATCG TGGGGACGGC GGGTCGCCGG GTGCTGGTGT GGGATCTGAG GAACATGGGA 
    TACGTGCAGC AGCGCCGCGA GTCCAGCCTC AAGTACCAGA CGCGCTGCAT ACGGGCCTTC 
    CCCAACAAAC AGGGCTATGT GCTGAGCTCC ATCGAGGGCC GTGTGGCTGT GGAGTATCTG 
    GACCCCAGTC TGGAGGTGCA GAAGAAGAAA TACGCCTTCA AGTGCCACAG ACTGAAGGAG 
    AACGGCATCG AGCAGGTGTA TCCGGTCAAC GCCATCTCCT TCCACAGCGT GCACAACACC 
    TTTGCCACCG GTGGTTCAGA CGGTTTCGTC AACATCTGGG ACCCGTTCAA TAAGAAGCGT 
    CTGTGTCAGT TCCACCGGTA CCCCAGCAGC ATCGCCTCTC TGTCCTTCAG CACGGACGGC 
    TCTCTGCTGG CCATCGCCTC CTCATACATG CAGGAGCTGG GGGACGTCTC ACACCCCGCT 
    GACGCTGTGT TCATCCGCCA GGTCACCGAT GCAGAGACCA AGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213932.1
    CDS82..1092
    Translation

    Target ORF information:

    RefSeq Version XM_017358443.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio BUB3 mitotic checkpoint protein (bub3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017358443.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA GAGCAGCAGC AGAGAACAGC GGCACGATGA CCGGAGCGAA TGAGTTTAAG 
    CTGGCCCAGG GCCCGGAGGA CAGCGTCTCG GCAGTGAAGT TCAGCCCGTC CTCCTCTCAG 
    TTCCTGCTGG TGTCCTCTTG GGACGGCTCC GTGCGGCTCT ACGACGCCAG CGCCAACAGC 
    ATGAGGATGA AGTACCAACA CCTGGCGCCC GTGCTGGACT GCGCTTTCTC TGACCCAACC 
    CATGCGTGGA GCGGTGGTCT GGACAGTCAG CTGAAGACTC ATGACCTGAA CACTGATCAG 
    GACACTATCG TGGGCACACA CGACGCCCCG ATCCGCTGTG TGGAGTTCTG CCCGGAGGTC 
    AATGTTCTGG TGACGGGCAG CTGGGATCAG TCGGTGCGGC TCTGGGACCC GCGAACACCC 
    TGCAACGCCG GCACATTCAC ACAGCCTGAG AAGGTGTACA CTCTCTCTGT GGCGGGGGAT 
    CGTCTGATCG TGGGGACGGC GGGTCGCCGG GTGCTGGTGT GGGATCTGAG GAACATGGGA 
    TACGTGCAGC AGCGCCGCGA GTCCAGCCTC AAGTACCAGA CGCGCTGCAT ACGGGCCTTC 
    CCCAACAAAC AGGGCTATGT GCTGAGCTCC ATCGAGGGCC GTGTGGCTGT GGAGTATCTG 
    GACCCCAGTC TGGAGGTGCA GAAGAAGAAA TACGCCTTCA AGTGCCACAG ACTGAAGGAG 
    AACGGCATCG AGCAGGTGTA TCCGGTCAAC GCCATCTCCT TCCACAGCGT GCACAACACC 
    TTTGCCACCG GTGGTTCAGA CGGTTTCGTC AACATCTGGG ACCCGTTCAA TAAGAAGCGT 
    CTGTGTCAGT TCCACCGGTA CCCCAGCAGC ATCGCCTCTC TGTCCTTCAG CACGGACGGC 
    TCTCTGCTGG CCATCGCCTC CTCATACATG CAGGAGCTGG GGGACGTCTC ACACCCCGCT 
    GACGCTGTGT TCATCCGCCA GGTCACCGAT GCAGAGACCA AGCCCAAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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