DSTYK cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DSTYK
Entrez Gene ID	402922
Full Name	dual serine/threonine and tyrosine protein kinase
Synonyms	ripk5,zgc:136421
General protein information	Preferred Names dual serine/threonine and tyrosine protein kinase Names dual serine/threonine and tyrosine protein kinase dusty PK dusty protein kinase receptor interacting protein kinase 5 receptor-interacting serine/threonine-protein kinase 5
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_205627.2	NP_991190.2	dual serine/threonine and tyrosine protein kinase
XM_005168270.4	XP_005168327.1	dual serine/threonine and tyrosine protein kinase isoform X1
XM_005168270.3	XP_005168327.1	dual serine/threonine and tyrosine protein kinase isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Dstyk	NP_955750.1
Gallus gallus (chicken)	DSTYK	NP_989837.1
Xenopus tropicalis (tropical clawed frog)	dstyk	NP_001007477.1
	DSTYK	NP_001035038.1
Pan troglodytes (chimpanzee)	DSTYK	NP_001029338.1
Bos taurus (cattle)	DSTYK	NP_001019995.1
Mus musculus (house mouse)	Dstyk	NP_766104.2
Danio rerio (zebrafish)	dstyk	NP_991190.2
Homo sapiens (human)	DSTYK	NP_056190.1
Canis lupus familiaris (dog)	DSTYK	NP_001020439.1

Gene Ontology
Bibliography

Related articles in PubMed

Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway.
Suarez-Bregua P, Torres-Nu?ez E, Saxena A, Guerreiro P, Braasch I, Prober DA, Moran P, Cerda-Reverter JM, Du SJ, Adrio F, Power DM, Canario AV, Postlethwait JH, Bronner ME, Ca?estro C, Rotllant J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(2)569-583(2017 02)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Mutations in DSTYK and dominant urinary tract malformations.
Sanna-Cherchi S, Sampogna RV, Papeta N, Burgess KE, Nees SN, Perry BJ, Choi M, Bodria M, Liu Y, Weng PL, Lozanovski VJ, Verbitsky M, Lugani F, Sterken R, Paragas N, Caridi G, Carrea A, Dagnino M, Materna-Kiryluk A, Santamaria G, Murtas C, Ristoska-Bojkovska N, Izzi C, Kacak N, Bianco B, Giberti S, Gigante M, Piaggio G, Gesualdo L, Vukic DK, Vukojevic K, Saraga-Babic M, Saraga M, Gucev Z, Allegri L, Latos-Bielenska A, Casu D, State M, Scolari F, Ravazzolo R, Kiryluk K, Al-Awqati Q, D'Agati VD, Drummond IA, Tasic V, Lifton RP, Ghiggeri GM, Gharavi AG
The New England journal of medicine369(7)621-9(2013 Aug)

Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish.
Stein C, Caccamo M, Laird G, Leptin M
Genome biology8(11)R251(2007)

Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain.
Peng J, Dong W, Chen Y, Mo R, Cheng JF, Hui CC, Mohandas N, Huang CH
Biochimica et biophysica acta1759(11-12)562-72(2006 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DSTYK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DSTYK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa38070	NM_205627.2 Latest version!	Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
ODa26320	XM_005168270.4 Latest version!	Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
ODa26320	XM_005168270.3	Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DSTYK ( NM_205627.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_991190.2 Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), mRNA.

Price & Availability↑

CloneID	ODa38070
Clone ID Related Accession (Same CDS sequence)	NM_205627.2
Accession Version	NM_205627.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	dual serine/threonine and tyrosine protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY429681.1. On Nov 15, 2004 this sequence version replaced NM_205627.1. ##Evidence-Data-START## Transcript exon combination :: AY429681.1, AY208855.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA3505383, SAMEA4476733 [ECO:0000350] ##Evidence-Data-END##

ATGGAGAACC CGCAGAAGCC CCGGGAGCTG ACCCGAGCCT TCAACCACTA CAACAAACAC 
AGCGGCTTCC TGAAGAAGAA CCTGAAGGAG ACCATCCTGT TCTTCAGGGA GATCCGACAG 
AACCACAGCA ACACCTGCGC TGCTGCAGAA CCAGGTCAGC TCAGCTGCAT CTCGTTCCCG 
CGTCAGGATG AGGAGTATCT GCAGAATGTG GTCAGCAGCG CCCCCTACAT CCTCATCCTG 
GGCCAGGACT GCAGCGCCCG CTACCAGCTG CTCAACTGCC TGCTGGGCGA GAGGCTCCTC 
CCCCTGGGGC CGGAGGCGGG CGGGGCCTGC GGCGCGGAGG GCGGGGCTTG CCGGCGGCGG 
AAGCTGTGTT TCACCCACGG CCGGCAGACG CGCCTGAGCC TGGCTCTTCC GGGACAGTAT 
GAGCTGGTGC ATCAGCTGGC GGCCCACTGC GGGCGCTGGG ACACGGTGCC CCGACAGGAC 
CTGGAGATCC AGGAGTGCGA AGATCCGGCG CAGCGATTGG CCGAGCTGGA GATCACGCTC 
CATCATACAC TGCTGCAGGA GGTGAAGATC ATGGTTCTGC CGTGTCGTAA CGTGCAGCCG 
CTGGAGGAGG CGCTGGAGGA CTGCAAGCGC GGGATCCTTC CCATCGTCCT GTACGCGGTG 
AGCAGGGAGA GTCTGAGCGC GCAGCAGCTG GAGGATCTGC AGACGCTGCG GGAGAGTCTG 
CCGTTCCCCG TCTGCTTCAT CCGCGTGTCG GACGGTGGCG GTGGCGGCGC TCTGTTCACG 
CAGCTAGCGT CGCTGCAGCT GATTAGCGCC TCCGCGGGGA ACTGCGCCTG CGGAGCTCCA 
GCAGCTCAAA GCGCCGGCCG CATGCAGGGT GTGCTGTGTG ACAGTCTGGA GCGGCTGCAG 
CGAGTCCTGG TGCCGTTCAC CCGACAGGTG CTGCAGAACC AGCAGGTGGA GGCGGCCACG 
CTCCTCAACA CCATACACTG CCGCTGCCTC GACCTCTTCA TCATACAGGC ATTCGACATG 
CAGAGGGATC TCCAGATCAC GCCGCGGCGT CTGGAGTACA CGCGGGAGAA GGAGGGCGAG 
CTGTTCTGCT CTCTGATGGC CATCGCCAAC CGCAAGCAGG AGGAGATGAA GGAGATGATC 
GTGGAGACGC TGAGCAGCAT GAAGGAGCAG CTGCTGGAGG ACGCGCAGAA CCTGGACTTC 
ACAGATATCA TCATGTCGTC TAACGGCGAG CCGGTGAGCT CTAAAGACAT CAAGGTGTGC 
ATCAGTCAGA TTCAGGATCT GATCGTCAAC AGACTCAATC AGGCGGTGGC CAACAAACTG 
ACCAACTCGG TGGATTACCT GCGGGAGAGC TTCGTGGGAA CACTGGAGAG ATGCCTCGGC 
AGCCTGGAGA AGTCCACACC TGAGTCCTGC GCACACAACG TCACGTCCAA CCATCTCAAA 
CAGATCATCC ACCGCATCAC ATGGGTCAAT CCTCCAGCCA TCACGGCCGA GTGGAAGCGG 
AAGGTCGCGC AGGACGCCAT CGAGAGCCTG AGCGCCGCCA AACTGGCCAA GAGCATCTGT 
TCCCAGTTCA GGACGAGGCT CAACAGCTCA CACGAGGCCT TCGCCGCATC GCTACGACAG 
CTGGAGGAGG GCCATACGGG GCGTCTGGAG CGAACTGAAG ATCTGTGGTT GCGGGTTCGG 
AAAGATCATG CGCCGAGACT CGCACGACTG TCACTGGAGA GCCGATCGCT GAGAGACATT 
CTGCTGCACG GGAAGCCAAA GCTGGGTCGT GAGCTGGGTC GTGGTCAGTA CGGTGTGGTG 
TATCTGTGTG ACAGCTGGGC TGGACGTCAC CCCTGCGCGC TCAAATCTGT GGTTCCGCCT 
GACGACAAAC ACTGGAACGA CCTGGCGCTG GAGTTCCACT ACACACGGTC TCTGCCGAAA 
CACGAGCGGT TAGTGAATCT GCACGGGTCG GTCATCGATC ACTCCTACAG CGGTGGATCC 
AGTATCGCTG TTCTGCTGAT CATGGAGCGC TTGCACAGAG ACCTCTATAC GGGACTGAAG 
GCGGGTCTGT CCCTGAAGGA GCGTCTTCTG ATCGCTCTGG ATGTGGTGGA GGGGATTCGC 
TTCCTGCACA GTCAGGGTCT CCTGCACCGC GACATCAAGC TGAAGAACGT CCTGCTGGAC 
AAGCAGAACC GGGCGAAGAT CACGGATCTG GGCTTCTGTA AACCCGAGGC CATGATGTCC 
GGCAGTATCG TGGGGACGCC CATCCACATG GCCCCAGAGT TATTCACAGG AAAGTACGAT 
AACTCGGTGG ACGTGTATGC GTTCGGGATA CTCTTCTGGT ATCTGTGCTC CGGCTCCGTC 
AAACTCCCAG AAGCCTTCGA GAAGTGCGCC AGTAAAGACC AGCTGTGGAC CAACGTCAAG 
AAAGGCTGTC GTCCGGAGCG TCTGCCGGTC TTTGATGAGG AGTGTTGGCA GCTGATGGAG 
GCGTGCTGGA ATGGAGATCC GTCGCAGCGC CCCCTGCTGG GCATCGTTCA ACCCGGCCTG 
CAGAGCATCA TGGAGAGACT GTGTGGAGAG AAGAGCCTGG AGGACTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_991190.2
CDS	46..2619
Translation	MENPQKPRELTRAFNHYNKHSGFLKKNLKETILFFREIRQNHSNTCAAAEPGQLSCISFPRQDEEYLQNVVSSAPYILILGQDCSARYQLLNCLLGERLLPLGPEAGGACGAEGGACRRRKLCFTHGRQTRLSLALPGQYELVHQLAAHCGRWDTVPRQDLEIQECEDPAQRLAELEITLHHTLLQEVKIMVLPCRNVQPLEEALEDCKRGILPIVLYAVSRESLSAQQLEDLQTLRESLPFPVCFIRVSDGGGGGALFTQLASLQLISASAGNCACGAPAAQSAGRMQGVLCDSLERLQRVLVPFTRQVLQNQQVEAATLLNTIHCRCLDLFIIQAFDMQRDLQITPRRLEYTREKEGELFCSLMAIANRKQEEMKEMIVETLSSMKEQLLEDAQNLDFTDIIMSSNGEPVSSKDIKVCISQIQDLIVNRLNQAVANKLTNSVDYLRESFVGTLERCLGSLEKSTPESCAHNVTSNHLKQIIHRITWVNPPAITAEWKRKVAQDAIESLSAAKLAKSICSQFRTRLNSSHEAFAASLRQLEEGHTGRLERTEDLWLRVRKDHAPRLARLSLESRSLRDILLHGKPKLGRELGRGQYGVVYLCDSWAGRHPCALKSVVPPDDKHWNDLALEFHYTRSLPKHERLVNLHGSVIDHSYSGGSSIAVLLIMERLHRDLYTGLKAGLSLKERLLIALDVVEGIRFLHSQGLLHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFTGKYDNSVDVYAFGILFWYLCSGSVKLPEAFEKCASKDQLWTNVKKGCRPERLPVFDEECWQLMEACWNGDPSQRPLLGIVQPGLQSIMERLCGEKSLEDSN

Target ORF information:

RefSeq Version	NM_205627.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205627.2

ORF Insert Sequence:

ATGGAGAACC CGCAGAAGCC CCGGGAGCTG ACCCGAGCCT TCAACCACTA CAACAAACAC 
AGCGGCTTCC TGAAGAAGAA CCTGAAGGAG ACCATCCTGT TCTTCAGGGA GATCCGACAG 
AACCACAGCA ACACCTGCGC TGCTGCAGAA CCAGGTCAGC TCAGCTGCAT CTCGTTCCCG 
CGTCAGGATG AGGAGTATCT GCAGAATGTG GTCAGCAGCG CCCCCTACAT CCTCATCCTG 
GGCCAGGACT GCAGCGCCCG CTACCAGCTG CTCAACTGCC TGCTGGGCGA GAGGCTCCTC 
CCCCTGGGGC CGGAGGCGGG CGGGGCCTGC GGCGCGGAGG GCGGGGCTTG CCGGCGGCGG 
AAGCTGTGTT TCACCCACGG CCGGCAGACG CGCCTGAGCC TGGCTCTTCC GGGACAGTAT 
GAGCTGGTGC ATCAGCTGGC GGCCCACTGC GGGCGCTGGG ACACGGTGCC CCGACAGGAC 
CTGGAGATCC AGGAGTGCGA AGATCCGGCG CAGCGATTGG CCGAGCTGGA GATCACGCTC 
CATCATACAC TGCTGCAGGA GGTGAAGATC ATGGTTCTGC CGTGTCGTAA CGTGCAGCCG 
CTGGAGGAGG CGCTGGAGGA CTGCAAGCGC GGGATCCTTC CCATCGTCCT GTACGCGGTG 
AGCAGGGAGA GTCTGAGCGC GCAGCAGCTG GAGGATCTGC AGACGCTGCG GGAGAGTCTG 
CCGTTCCCCG TCTGCTTCAT CCGCGTGTCG GACGGTGGCG GTGGCGGCGC TCTGTTCACG 
CAGCTAGCGT CGCTGCAGCT GATTAGCGCC TCCGCGGGGA ACTGCGCCTG CGGAGCTCCA 
GCAGCTCAAA GCGCCGGCCG CATGCAGGGT GTGCTGTGTG ACAGTCTGGA GCGGCTGCAG 
CGAGTCCTGG TGCCGTTCAC CCGACAGGTG CTGCAGAACC AGCAGGTGGA GGCGGCCACG 
CTCCTCAACA CCATACACTG CCGCTGCCTC GACCTCTTCA TCATACAGGC ATTCGACATG 
CAGAGGGATC TCCAGATCAC GCCGCGGCGT CTGGAGTACA CGCGGGAGAA GGAGGGCGAG 
CTGTTCTGCT CTCTGATGGC CATCGCCAAC CGCAAGCAGG AGGAGATGAA GGAGATGATC 
GTGGAGACGC TGAGCAGCAT GAAGGAGCAG CTGCTGGAGG ACGCGCAGAA CCTGGACTTC 
ACAGATATCA TCATGTCGTC TAACGGCGAG CCGGTGAGCT CTAAAGACAT CAAGGTGTGC 
ATCAGTCAGA TTCAGGATCT GATCGTCAAC AGACTCAATC AGGCGGTGGC CAACAAACTG 
ACCAACTCGG TGGATTACCT GCGGGAGAGC TTCGTGGGAA CACTGGAGAG ATGCCTCGGC 
AGCCTGGAGA AGTCCACACC TGAGTCCTGC GCACACAACG TCACGTCCAA CCATCTCAAA 
CAGATCATCC ACCGCATCAC ATGGGTCAAT CCTCCAGCCA TCACGGCCGA GTGGAAGCGG 
AAGGTCGCGC AGGACGCCAT CGAGAGCCTG AGCGCCGCCA AACTGGCCAA GAGCATCTGT 
TCCCAGTTCA GGACGAGGCT CAACAGCTCA CACGAGGCCT TCGCCGCATC GCTACGACAG 
CTGGAGGAGG GCCATACGGG GCGTCTGGAG CGAACTGAAG ATCTGTGGTT GCGGGTTCGG 
AAAGATCATG CGCCGAGACT CGCACGACTG TCACTGGAGA GCCGATCGCT GAGAGACATT 
CTGCTGCACG GGAAGCCAAA GCTGGGTCGT GAGCTGGGTC GTGGTCAGTA CGGTGTGGTG 
TATCTGTGTG ACAGCTGGGC TGGACGTCAC CCCTGCGCGC TCAAATCTGT GGTTCCGCCT 
GACGACAAAC ACTGGAACGA CCTGGCGCTG GAGTTCCACT ACACACGGTC TCTGCCGAAA 
CACGAGCGGT TAGTGAATCT GCACGGGTCG GTCATCGATC ACTCCTACAG CGGTGGATCC 
AGTATCGCTG TTCTGCTGAT CATGGAGCGC TTGCACAGAG ACCTCTATAC GGGACTGAAG 
GCGGGTCTGT CCCTGAAGGA GCGTCTTCTG ATCGCTCTGG ATGTGGTGGA GGGGATTCGC 
TTCCTGCACA GTCAGGGTCT CCTGCACCGC GACATCAAGC TGAAGAACGT CCTGCTGGAC 
AAGCAGAACC GGGCGAAGAT CACGGATCTG GGCTTCTGTA AACCCGAGGC CATGATGTCC 
GGCAGTATCG TGGGGACGCC CATCCACATG GCCCCAGAGT TATTCACAGG AAAGTACGAT 
AACTCGGTGG ACGTGTATGC GTTCGGGATA CTCTTCTGGT ATCTGTGCTC CGGCTCCGTC 
AAACTCCCAG AAGCCTTCGA GAAGTGCGCC AGTAAAGACC AGCTGTGGAC CAACGTCAAG 
AAAGGCTGTC GTCCGGAGCG TCTGCCGGTC TTTGATGAGG AGTGTTGGCA GCTGATGGAG 
GCGTGCTGGA ATGGAGATCC GTCGCAGCGC CCCCTGCTGG GCATCGTTCA ACCCGGCCTG 
CAGAGCATCA TGGAGAGACT GTGTGGAGAG AAGAGCCTGG AGGACTCCAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DSTYK ( XM_005168270.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168327.1 Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa26320
Clone ID Related Accession (Same CDS sequence)	XM_005168270.4 , XM_005168270.3
Accession Version	XM_005168270.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dual serine/threonine and tyrosine protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168270.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC CGCAGAAGCC CCGGGAGCTG ACCCGAGCCT TCAACCACTA CAACAAACAC 
AGCGGCTTCC TGAAGAAGAA CCTGAAGGAG ACCATCCTGT TCTTCAGGGA GATCCGACAG 
AACCACAGCA ACACCTGCGC TGCTGCAGAA CCAGGTCAGC TCAGCTGCAT CTCGTTCCCG 
CGTCAGGATG AGGAGTATCT GCAGAATGTG GTCAGCAGCG CCCCCTACAT CCTCATCCTG 
GGCCAGGACT GCAGCGCCCG CTACCAGCTG CTCAACTGCC TGCTGGGCGA GAGGCTCCTC 
CCCCTGGGGC CGGAGGCGGG CGGGGCCTGC GGCGCGGAGG GCGGGGCTTG CCGGCGGCGG 
AAGCTGTGTT TCACCCACGG CCGGCAGACG CGCCTGAGCC TGGCTCTTCC GGGACAGTAT 
GAGCTGGTGC ATCAGCTGGC GGCCCACTGC GGGCGCTGGG ACACGGTGCC CCGACAGGAC 
CTGGAGATCC AGGAGTGCGA AGATCCGGCG CAGCGATTGG CCGAGCTGGA GATCACGCTC 
CATCATACAC TGCTGCAGGA GGTGAAGATC ATGGTTCTGC CGTGTCGTAA CGTGCAGCCG 
CTGGAGGAGG CGCTGGAGGA CTGCAAGCGC GGGATCCTTC CCATCGTCCT GTACGCGGTG 
AGCAGGGAGA GTCTGAGCGC GCAGCAGCTG GAGGATCTGC AGACGCTGCG GGAGAGTCTG 
CCGTTCCCCG TCTGCTTCAT CCGCGTGTCG GACGGTGGCG GTGGCGGCGC TCTGTTCACG 
CAGCTAGCGT CGCTGCAGCT GATTAGCGCC TCCGCGGGGA ACTGCGCCTG CGGAGCTCCA 
GCAGCTCAGA GCGCCGGCCG CATGCAGGGT GTGCTGTGTG ACAGTCTGGA GCGGCTGCAG 
CGAGTCCTGG TGCCGTTCAC CCGACAGGTG CTGCAGAACC AGCAGGTGGA GGCGGCCACG 
CTCCTCAACA CCATACACTG CCGCTGCCTC GACCTCTTCA TCATACAGGC ATTCGACATG 
CAGAGGGATC TCCAGATCAC GCCGCGGCGT CTGGAGTACA CGCGGGAGAA GGAGGGCGAG 
CTGTTCTGCT CTCTGATGGC CATCGCCAAC CGCAAGCAGG AGGAGATGAA GGAGATGATC 
GTGGAGACGC TGAGCAGCAT GAAGGAGCAG CTGCTGGAGG ACGCGCAGAA CCTGGACTTC 
ACAGATATCA TCATGTCGTC TAACGGCGAG CCGGTGAGCT CTAAAGACAT CAAGGTGTGC 
ATCAGTCAGA TTCAGGATCT GATCGTCAAC AGACTCAATC AGGCGGTGGC CAACAAACTG 
ACCAACTCGG TGGATTACCT GCGGGAGAGC TTCGTGGGAA CACTGGAGAG ATGCCTCGGC 
AGCCTGGAGA AGTCCACACC TGAGTCCTGC GCACACAACG TCACGTCCAA CCATCTCAAA 
CAGATCCTGA ATGCGGCGTA TCACGTGGAG GTCACCTTCC ACTCCGGTTC ATCCGTCACG 
CGTCTCTTCT GGGAGCAGAT CAAACAGATC ATCCACCGCA TCACATGGGT CAATCCTCCA 
GCCATCACGG CCGAGTGGAA GCGGAAGGTC GCGCAGGACG CCATCGAGAG CCTGAGCGCC 
GCCAAACTGG CCAAGAGCAT CTGTTCCCAG TTCAGGACGA GGCTCAACAG CTCACACGAG 
GCCTTCGCCG CATCGCTACG ACAGCTGGAG GAGGGCCATA CGGGGCGTCT GGAGCGAACT 
GAAGATCTGT GGTTGCGGGT TCGGAAAGAT CATGCGCCGA GACTCGCACG ACTGTCACTG 
GAGAGCCGAT CGCTGAGAGA CATTCTGCTG CACGGGAAGC CAAAGCTGGG TCGTGAGCTG 
GGTCGTGGTC AGTACGGTGT GGTGTATCTG TGTGACAGCT GGGCTGGACG TCACCCCTGC 
GCGCTCAAAT CTGTGGTTCC GCCTGACGAC AAACACTGGA ACGACCTGGC GCTGGAGTTC 
CACTACACAC GGTCTCTGCC GAAACACGAG CGGTTAGTGA ATCTGCACGG GTCGGTCATC 
GATCACTCCT ACAGCGGTGG ATCCAGTATC GCTGTTCTGC TGATCATGGA GCGCTTGCAC 
AGAGACCTCT ATACGGGACT GAAGGCGGGT CTGTCCCTGA AGGAGCGTCT TCTGATCGCT 
CTGGATGTGG TGGAGGGGAT TCGCTTCCTG CACAGTCAGG GTCTCCTGCA CCGCGACATC 
AAGCTGAAGA ACGTCCTGCT GGACAAGCAG AACCGGGCGA AGATCACGGA TCTGGGCTTC 
TGTAAACCCG AGGCCATGAT GTCCGGCAGT ATCGTGGGGA CGCCCATCCA CATGGCCCCA 
GAGTTATTCA CAGGAAAGTA CGATAACTCG GTGGACGTGT ATGCGTTCGG GATACTCTTC 
TGGTATCTGT GCTCCGGCTC CGTCAAACTC CCAGAAGCCT TCGAGAAGTG CGCCAGTAAA 
GACCAGCTGT GGACCAACGT CAAGAAAGGC TGTCGTCCGG AGCGTCTGCC GGTCTTTGAT 
GAGGAGTGTT GGCAGCTGAT GGAGGCGTGC TGGAATGGAG ATCCGTCGCA GCGCCCCCTG 
CTGGGCATCG TTCAACCCGG CCTGCAGAGC ATCATGGAGA GACTGTGTGG AGAGAAGAGC 
CTGGAGGACT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168327.1
CDS	444..3101
Translation	MENPQKPRELTRAFNHYNKHSGFLKKNLKETILFFREIRQNHSNTCAAAEPGQLSCISFPRQDEEYLQNVVSSAPYILILGQDCSARYQLLNCLLGERLLPLGPEAGGACGAEGGACRRRKLCFTHGRQTRLSLALPGQYELVHQLAAHCGRWDTVPRQDLEIQECEDPAQRLAELEITLHHTLLQEVKIMVLPCRNVQPLEEALEDCKRGILPIVLYAVSRESLSAQQLEDLQTLRESLPFPVCFIRVSDGGGGGALFTQLASLQLISASAGNCACGAPAAQSAGRMQGVLCDSLERLQRVLVPFTRQVLQNQQVEAATLLNTIHCRCLDLFIIQAFDMQRDLQITPRRLEYTREKEGELFCSLMAIANRKQEEMKEMIVETLSSMKEQLLEDAQNLDFTDIIMSSNGEPVSSKDIKVCISQIQDLIVNRLNQAVANKLTNSVDYLRESFVGTLERCLGSLEKSTPESCAHNVTSNHLKQILNAAYHVEVTFHSGSSVTRLFWEQIKQIIHRITWVNPPAITAEWKRKVAQDAIESLSAAKLAKSICSQFRTRLNSSHEAFAASLRQLEEGHTGRLERTEDLWLRVRKDHAPRLARLSLESRSLRDILLHGKPKLGRELGRGQYGVVYLCDSWAGRHPCALKSVVPPDDKHWNDLALEFHYTRSLPKHERLVNLHGSVIDHSYSGGSSIAVLLIMERLHRDLYTGLKAGLSLKERLLIALDVVEGIRFLHSQGLLHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFTGKYDNSVDVYAFGILFWYLCSGSVKLPEAFEKCASKDQLWTNVKKGCRPERLPVFDEECWQLMEACWNGDPSQRPLLGIVQPGLQSIMERLCGEKSLEDSN

Target ORF information:

RefSeq Version	XM_005168270.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168270.4

ORF Insert Sequence:

ATGGAGAACC CGCAGAAGCC CCGGGAGCTG ACCCGAGCCT TCAACCACTA CAACAAACAC 
AGCGGCTTCC TGAAGAAGAA CCTGAAGGAG ACCATCCTGT TCTTCAGGGA GATCCGACAG 
AACCACAGCA ACACCTGCGC TGCTGCAGAA CCAGGTCAGC TCAGCTGCAT CTCGTTCCCG 
CGTCAGGATG AGGAGTATCT GCAGAATGTG GTCAGCAGCG CCCCCTACAT CCTCATCCTG 
GGCCAGGACT GCAGCGCCCG CTACCAGCTG CTCAACTGCC TGCTGGGCGA GAGGCTCCTC 
CCCCTGGGGC CGGAGGCGGG CGGGGCCTGC GGCGCGGAGG GCGGGGCTTG CCGGCGGCGG 
AAGCTGTGTT TCACCCACGG CCGGCAGACG CGCCTGAGCC TGGCTCTTCC GGGACAGTAT 
GAGCTGGTGC ATCAGCTGGC GGCCCACTGC GGGCGCTGGG ACACGGTGCC CCGACAGGAC 
CTGGAGATCC AGGAGTGCGA AGATCCGGCG CAGCGATTGG CCGAGCTGGA GATCACGCTC 
CATCATACAC TGCTGCAGGA GGTGAAGATC ATGGTTCTGC CGTGTCGTAA CGTGCAGCCG 
CTGGAGGAGG CGCTGGAGGA CTGCAAGCGC GGGATCCTTC CCATCGTCCT GTACGCGGTG 
AGCAGGGAGA GTCTGAGCGC GCAGCAGCTG GAGGATCTGC AGACGCTGCG GGAGAGTCTG 
CCGTTCCCCG TCTGCTTCAT CCGCGTGTCG GACGGTGGCG GTGGCGGCGC TCTGTTCACG 
CAGCTAGCGT CGCTGCAGCT GATTAGCGCC TCCGCGGGGA ACTGCGCCTG CGGAGCTCCA 
GCAGCTCAGA GCGCCGGCCG CATGCAGGGT GTGCTGTGTG ACAGTCTGGA GCGGCTGCAG 
CGAGTCCTGG TGCCGTTCAC CCGACAGGTG CTGCAGAACC AGCAGGTGGA GGCGGCCACG 
CTCCTCAACA CCATACACTG CCGCTGCCTC GACCTCTTCA TCATACAGGC ATTCGACATG 
CAGAGGGATC TCCAGATCAC GCCGCGGCGT CTGGAGTACA CGCGGGAGAA GGAGGGCGAG 
CTGTTCTGCT CTCTGATGGC CATCGCCAAC CGCAAGCAGG AGGAGATGAA GGAGATGATC 
GTGGAGACGC TGAGCAGCAT GAAGGAGCAG CTGCTGGAGG ACGCGCAGAA CCTGGACTTC 
ACAGATATCA TCATGTCGTC TAACGGCGAG CCGGTGAGCT CTAAAGACAT CAAGGTGTGC 
ATCAGTCAGA TTCAGGATCT GATCGTCAAC AGACTCAATC AGGCGGTGGC CAACAAACTG 
ACCAACTCGG TGGATTACCT GCGGGAGAGC TTCGTGGGAA CACTGGAGAG ATGCCTCGGC 
AGCCTGGAGA AGTCCACACC TGAGTCCTGC GCACACAACG TCACGTCCAA CCATCTCAAA 
CAGATCCTGA ATGCGGCGTA TCACGTGGAG GTCACCTTCC ACTCCGGTTC ATCCGTCACG 
CGTCTCTTCT GGGAGCAGAT CAAACAGATC ATCCACCGCA TCACATGGGT CAATCCTCCA 
GCCATCACGG CCGAGTGGAA GCGGAAGGTC GCGCAGGACG CCATCGAGAG CCTGAGCGCC 
GCCAAACTGG CCAAGAGCAT CTGTTCCCAG TTCAGGACGA GGCTCAACAG CTCACACGAG 
GCCTTCGCCG CATCGCTACG ACAGCTGGAG GAGGGCCATA CGGGGCGTCT GGAGCGAACT 
GAAGATCTGT GGTTGCGGGT TCGGAAAGAT CATGCGCCGA GACTCGCACG ACTGTCACTG 
GAGAGCCGAT CGCTGAGAGA CATTCTGCTG CACGGGAAGC CAAAGCTGGG TCGTGAGCTG 
GGTCGTGGTC AGTACGGTGT GGTGTATCTG TGTGACAGCT GGGCTGGACG TCACCCCTGC 
GCGCTCAAAT CTGTGGTTCC GCCTGACGAC AAACACTGGA ACGACCTGGC GCTGGAGTTC 
CACTACACAC GGTCTCTGCC GAAACACGAG CGGTTAGTGA ATCTGCACGG GTCGGTCATC 
GATCACTCCT ACAGCGGTGG ATCCAGTATC GCTGTTCTGC TGATCATGGA GCGCTTGCAC 
AGAGACCTCT ATACGGGACT GAAGGCGGGT CTGTCCCTGA AGGAGCGTCT TCTGATCGCT 
CTGGATGTGG TGGAGGGGAT TCGCTTCCTG CACAGTCAGG GTCTCCTGCA CCGCGACATC 
AAGCTGAAGA ACGTCCTGCT GGACAAGCAG AACCGGGCGA AGATCACGGA TCTGGGCTTC 
TGTAAACCCG AGGCCATGAT GTCCGGCAGT ATCGTGGGGA CGCCCATCCA CATGGCCCCA 
GAGTTATTCA CAGGAAAGTA CGATAACTCG GTGGACGTGT ATGCGTTCGG GATACTCTTC 
TGGTATCTGT GCTCCGGCTC CGTCAAACTC CCAGAAGCCT TCGAGAAGTG CGCCAGTAAA 
GACCAGCTGT GGACCAACGT CAAGAAAGGC TGTCGTCCGG AGCGTCTGCC GGTCTTTGAT 
GAGGAGTGTT GGCAGCTGAT GGAGGCGTGC TGGAATGGAG ATCCGTCGCA GCGCCCCCTG 
CTGGGCATCG TTCAACCCGG CCTGCAGAGC ATCATGGAGA GACTGTGTGG AGAGAAGAGC 
CTGGAGGACT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DSTYK ( XM_005168270.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005168327.1 Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), transcript variant X1, mRNA.

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CloneID	ODa26320
Clone ID Related Accession (Same CDS sequence)	XM_005168270.4 , XM_005168270.3
Accession Version	XM_005168270.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dual serine/threonine and tyrosine protein kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACC CGCAGAAGCC CCGGGAGCTG ACCCGAGCCT TCAACCACTA CAACAAACAC 
AGCGGCTTCC TGAAGAAGAA CCTGAAGGAG ACCATCCTGT TCTTCAGGGA GATCCGACAG 
AACCACAGCA ACACCTGCGC TGCTGCAGAA CCAGGTCAGC TCAGCTGCAT CTCGTTCCCG 
CGTCAGGATG AGGAGTATCT GCAGAATGTG GTCAGCAGCG CCCCCTACAT CCTCATCCTG 
GGCCAGGACT GCAGCGCCCG CTACCAGCTG CTCAACTGCC TGCTGGGCGA GAGGCTCCTC 
CCCCTGGGGC CGGAGGCGGG CGGGGCCTGC GGCGCGGAGG GCGGGGCTTG CCGGCGGCGG 
AAGCTGTGTT TCACCCACGG CCGGCAGACG CGCCTGAGCC TGGCTCTTCC GGGACAGTAT 
GAGCTGGTGC ATCAGCTGGC GGCCCACTGC GGGCGCTGGG ACACGGTGCC CCGACAGGAC 
CTGGAGATCC AGGAGTGCGA AGATCCGGCG CAGCGATTGG CCGAGCTGGA GATCACGCTC 
CATCATACAC TGCTGCAGGA GGTGAAGATC ATGGTTCTGC CGTGTCGTAA CGTGCAGCCG 
CTGGAGGAGG CGCTGGAGGA CTGCAAGCGC GGGATCCTTC CCATCGTCCT GTACGCGGTG 
AGCAGGGAGA GTCTGAGCGC GCAGCAGCTG GAGGATCTGC AGACGCTGCG GGAGAGTCTG 
CCGTTCCCCG TCTGCTTCAT CCGCGTGTCG GACGGTGGCG GTGGCGGCGC TCTGTTCACG 
CAGCTAGCGT CGCTGCAGCT GATTAGCGCC TCCGCGGGGA ACTGCGCCTG CGGAGCTCCA 
GCAGCTCAGA GCGCCGGCCG CATGCAGGGT GTGCTGTGTG ACAGTCTGGA GCGGCTGCAG 
CGAGTCCTGG TGCCGTTCAC CCGACAGGTG CTGCAGAACC AGCAGGTGGA GGCGGCCACG 
CTCCTCAACA CCATACACTG CCGCTGCCTC GACCTCTTCA TCATACAGGC ATTCGACATG 
CAGAGGGATC TCCAGATCAC GCCGCGGCGT CTGGAGTACA CGCGGGAGAA GGAGGGCGAG 
CTGTTCTGCT CTCTGATGGC CATCGCCAAC CGCAAGCAGG AGGAGATGAA GGAGATGATC 
GTGGAGACGC TGAGCAGCAT GAAGGAGCAG CTGCTGGAGG ACGCGCAGAA CCTGGACTTC 
ACAGATATCA TCATGTCGTC TAACGGCGAG CCGGTGAGCT CTAAAGACAT CAAGGTGTGC 
ATCAGTCAGA TTCAGGATCT GATCGTCAAC AGACTCAATC AGGCGGTGGC CAACAAACTG 
ACCAACTCGG TGGATTACCT GCGGGAGAGC TTCGTGGGAA CACTGGAGAG ATGCCTCGGC 
AGCCTGGAGA AGTCCACACC TGAGTCCTGC GCACACAACG TCACGTCCAA CCATCTCAAA 
CAGATCCTGA ATGCGGCGTA TCACGTGGAG GTCACCTTCC ACTCCGGTTC ATCCGTCACG 
CGTCTCTTCT GGGAGCAGAT CAAACAGATC ATCCACCGCA TCACATGGGT CAATCCTCCA 
GCCATCACGG CCGAGTGGAA GCGGAAGGTC GCGCAGGACG CCATCGAGAG CCTGAGCGCC 
GCCAAACTGG CCAAGAGCAT CTGTTCCCAG TTCAGGACGA GGCTCAACAG CTCACACGAG 
GCCTTCGCCG CATCGCTACG ACAGCTGGAG GAGGGCCATA CGGGGCGTCT GGAGCGAACT 
GAAGATCTGT GGTTGCGGGT TCGGAAAGAT CATGCGCCGA GACTCGCACG ACTGTCACTG 
GAGAGCCGAT CGCTGAGAGA CATTCTGCTG CACGGGAAGC CAAAGCTGGG TCGTGAGCTG 
GGTCGTGGTC AGTACGGTGT GGTGTATCTG TGTGACAGCT GGGCTGGACG TCACCCCTGC 
GCGCTCAAAT CTGTGGTTCC GCCTGACGAC AAACACTGGA ACGACCTGGC GCTGGAGTTC 
CACTACACAC GGTCTCTGCC GAAACACGAG CGGTTAGTGA ATCTGCACGG GTCGGTCATC 
GATCACTCCT ACAGCGGTGG ATCCAGTATC GCTGTTCTGC TGATCATGGA GCGCTTGCAC 
AGAGACCTCT ATACGGGACT GAAGGCGGGT CTGTCCCTGA AGGAGCGTCT TCTGATCGCT 
CTGGATGTGG TGGAGGGGAT TCGCTTCCTG CACAGTCAGG GTCTCCTGCA CCGCGACATC 
AAGCTGAAGA ACGTCCTGCT GGACAAGCAG AACCGGGCGA AGATCACGGA TCTGGGCTTC 
TGTAAACCCG AGGCCATGAT GTCCGGCAGT ATCGTGGGGA CGCCCATCCA CATGGCCCCA 
GAGTTATTCA CAGGAAAGTA CGATAACTCG GTGGACGTGT ATGCGTTCGG GATACTCTTC 
TGGTATCTGT GCTCCGGCTC CGTCAAACTC CCAGAAGCCT TCGAGAAGTG CGCCAGTAAA 
GACCAGCTGT GGACCAACGT CAAGAAAGGC TGTCGTCCGG AGCGTCTGCC GGTCTTTGAT 
GAGGAGTGTT GGCAGCTGAT GGAGGCGTGC TGGAATGGAG ATCCGTCGCA GCGCCCCCTG 
CTGGGCATCG TTCAACCCGG CCTGCAGAGC ATCATGGAGA GACTGTGTGG AGAGAAGAGC 
CTGGAGGACT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168327.1
CDS	448..3105
Translation	MENPQKPRELTRAFNHYNKHSGFLKKNLKETILFFREIRQNHSNTCAAAEPGQLSCISFPRQDEEYLQNVVSSAPYILILGQDCSARYQLLNCLLGERLLPLGPEAGGACGAEGGACRRRKLCFTHGRQTRLSLALPGQYELVHQLAAHCGRWDTVPRQDLEIQECEDPAQRLAELEITLHHTLLQEVKIMVLPCRNVQPLEEALEDCKRGILPIVLYAVSRESLSAQQLEDLQTLRESLPFPVCFIRVSDGGGGGALFTQLASLQLISASAGNCACGAPAAQSAGRMQGVLCDSLERLQRVLVPFTRQVLQNQQVEAATLLNTIHCRCLDLFIIQAFDMQRDLQITPRRLEYTREKEGELFCSLMAIANRKQEEMKEMIVETLSSMKEQLLEDAQNLDFTDIIMSSNGEPVSSKDIKVCISQIQDLIVNRLNQAVANKLTNSVDYLRESFVGTLERCLGSLEKSTPESCAHNVTSNHLKQILNAAYHVEVTFHSGSSVTRLFWEQIKQIIHRITWVNPPAITAEWKRKVAQDAIESLSAAKLAKSICSQFRTRLNSSHEAFAASLRQLEEGHTGRLERTEDLWLRVRKDHAPRLARLSLESRSLRDILLHGKPKLGRELGRGQYGVVYLCDSWAGRHPCALKSVVPPDDKHWNDLALEFHYTRSLPKHERLVNLHGSVIDHSYSGGSSIAVLLIMERLHRDLYTGLKAGLSLKERLLIALDVVEGIRFLHSQGLLHRDIKLKNVLLDKQNRAKITDLGFCKPEAMMSGSIVGTPIHMAPELFTGKYDNSVDVYAFGILFWYLCSGSVKLPEAFEKCASKDQLWTNVKKGCRPERLPVFDEECWQLMEACWNGDPSQRPLLGIVQPGLQSIMERLCGEKSLEDSN

Target ORF information:

RefSeq Version	XM_005168270.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dual serine/threonine and tyrosine protein kinase (dstyk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168270.3

ORF Insert Sequence:

ATGGAGAACC CGCAGAAGCC CCGGGAGCTG ACCCGAGCCT TCAACCACTA CAACAAACAC 
AGCGGCTTCC TGAAGAAGAA CCTGAAGGAG ACCATCCTGT TCTTCAGGGA GATCCGACAG 
AACCACAGCA ACACCTGCGC TGCTGCAGAA CCAGGTCAGC TCAGCTGCAT CTCGTTCCCG 
CGTCAGGATG AGGAGTATCT GCAGAATGTG GTCAGCAGCG CCCCCTACAT CCTCATCCTG 
GGCCAGGACT GCAGCGCCCG CTACCAGCTG CTCAACTGCC TGCTGGGCGA GAGGCTCCTC 
CCCCTGGGGC CGGAGGCGGG CGGGGCCTGC GGCGCGGAGG GCGGGGCTTG CCGGCGGCGG 
AAGCTGTGTT TCACCCACGG CCGGCAGACG CGCCTGAGCC TGGCTCTTCC GGGACAGTAT 
GAGCTGGTGC ATCAGCTGGC GGCCCACTGC GGGCGCTGGG ACACGGTGCC CCGACAGGAC 
CTGGAGATCC AGGAGTGCGA AGATCCGGCG CAGCGATTGG CCGAGCTGGA GATCACGCTC 
CATCATACAC TGCTGCAGGA GGTGAAGATC ATGGTTCTGC CGTGTCGTAA CGTGCAGCCG 
CTGGAGGAGG CGCTGGAGGA CTGCAAGCGC GGGATCCTTC CCATCGTCCT GTACGCGGTG 
AGCAGGGAGA GTCTGAGCGC GCAGCAGCTG GAGGATCTGC AGACGCTGCG GGAGAGTCTG 
CCGTTCCCCG TCTGCTTCAT CCGCGTGTCG GACGGTGGCG GTGGCGGCGC TCTGTTCACG 
CAGCTAGCGT CGCTGCAGCT GATTAGCGCC TCCGCGGGGA ACTGCGCCTG CGGAGCTCCA 
GCAGCTCAGA GCGCCGGCCG CATGCAGGGT GTGCTGTGTG ACAGTCTGGA GCGGCTGCAG 
CGAGTCCTGG TGCCGTTCAC CCGACAGGTG CTGCAGAACC AGCAGGTGGA GGCGGCCACG 
CTCCTCAACA CCATACACTG CCGCTGCCTC GACCTCTTCA TCATACAGGC ATTCGACATG 
CAGAGGGATC TCCAGATCAC GCCGCGGCGT CTGGAGTACA CGCGGGAGAA GGAGGGCGAG 
CTGTTCTGCT CTCTGATGGC CATCGCCAAC CGCAAGCAGG AGGAGATGAA GGAGATGATC 
GTGGAGACGC TGAGCAGCAT GAAGGAGCAG CTGCTGGAGG ACGCGCAGAA CCTGGACTTC 
ACAGATATCA TCATGTCGTC TAACGGCGAG CCGGTGAGCT CTAAAGACAT CAAGGTGTGC 
ATCAGTCAGA TTCAGGATCT GATCGTCAAC AGACTCAATC AGGCGGTGGC CAACAAACTG 
ACCAACTCGG TGGATTACCT GCGGGAGAGC TTCGTGGGAA CACTGGAGAG ATGCCTCGGC 
AGCCTGGAGA AGTCCACACC TGAGTCCTGC GCACACAACG TCACGTCCAA CCATCTCAAA 
CAGATCCTGA ATGCGGCGTA TCACGTGGAG GTCACCTTCC ACTCCGGTTC ATCCGTCACG 
CGTCTCTTCT GGGAGCAGAT CAAACAGATC ATCCACCGCA TCACATGGGT CAATCCTCCA 
GCCATCACGG CCGAGTGGAA GCGGAAGGTC GCGCAGGACG CCATCGAGAG CCTGAGCGCC 
GCCAAACTGG CCAAGAGCAT CTGTTCCCAG TTCAGGACGA GGCTCAACAG CTCACACGAG 
GCCTTCGCCG CATCGCTACG ACAGCTGGAG GAGGGCCATA CGGGGCGTCT GGAGCGAACT 
GAAGATCTGT GGTTGCGGGT TCGGAAAGAT CATGCGCCGA GACTCGCACG ACTGTCACTG 
GAGAGCCGAT CGCTGAGAGA CATTCTGCTG CACGGGAAGC CAAAGCTGGG TCGTGAGCTG 
GGTCGTGGTC AGTACGGTGT GGTGTATCTG TGTGACAGCT GGGCTGGACG TCACCCCTGC 
GCGCTCAAAT CTGTGGTTCC GCCTGACGAC AAACACTGGA ACGACCTGGC GCTGGAGTTC 
CACTACACAC GGTCTCTGCC GAAACACGAG CGGTTAGTGA ATCTGCACGG GTCGGTCATC 
GATCACTCCT ACAGCGGTGG ATCCAGTATC GCTGTTCTGC TGATCATGGA GCGCTTGCAC 
AGAGACCTCT ATACGGGACT GAAGGCGGGT CTGTCCCTGA AGGAGCGTCT TCTGATCGCT 
CTGGATGTGG TGGAGGGGAT TCGCTTCCTG CACAGTCAGG GTCTCCTGCA CCGCGACATC 
AAGCTGAAGA ACGTCCTGCT GGACAAGCAG AACCGGGCGA AGATCACGGA TCTGGGCTTC 
TGTAAACCCG AGGCCATGAT GTCCGGCAGT ATCGTGGGGA CGCCCATCCA CATGGCCCCA 
GAGTTATTCA CAGGAAAGTA CGATAACTCG GTGGACGTGT ATGCGTTCGG GATACTCTTC 
TGGTATCTGT GCTCCGGCTC CGTCAAACTC CCAGAAGCCT TCGAGAAGTG CGCCAGTAAA 
GACCAGCTGT GGACCAACGT CAAGAAAGGC TGTCGTCCGG AGCGTCTGCC GGTCTTTGAT 
GAGGAGTGTT GGCAGCTGAT GGAGGCGTGC TGGAATGGAG ATCCGTCGCA GCGCCCCCTG 
CTGGGCATCG TTCAACCCGG CCTGCAGAGC ATCATGGAGA GACTGTGTGG AGAGAAGAGC 
CTGGAGGACT CCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pth4, an ancient parathyroid hormone lost in eutherian mammals, reveals a new brain-to-bone signaling pathway.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(2)569-583(2017 02)
Suarez-Bregua P,Torres-Nu?ez E,Saxena A,Guerreiro P,Braasch I,Prober DA,Moran P,Cerda-Reverter JM,Du SJ,Adrio F,Power DM,Canario AV,Postlethwait JH,Bronner ME,Ca?estro C,Rotllant J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Mutations in DSTYK and dominant urinary tract malformations.
The New England journal of medicine369(7)621-9(2013 Aug)
Sanna-Cherchi S,Sampogna RV,Papeta N,Burgess KE,Nees SN,Perry BJ,Choi M,Bodria M,Liu Y,Weng PL,Lozanovski VJ,Verbitsky M,Lugani F,Sterken R,Paragas N,Caridi G,Carrea A,Dagnino M,Materna-Kiryluk A,Santamaria G,Murtas C,Ristoska-Bojkovska N,Izzi C,Kacak N,Bianco B,Giberti S,Gigante M,Piaggio G,Gesualdo L,Vukic DK,Vukojevic K,Saraga-Babic M,Saraga M,Gucev Z,Allegri L,Latos-Bielenska A,Casu D,State M,Scolari F,Ravazzolo R,Kiryluk K,Al-Awqati Q,D'Agati VD,Drummond IA,Tasic V,Lifton RP,Ghiggeri GM,Gharavi AG

Conservation and divergence of gene families encoding components of innate immune response systems in zebrafish.
Genome biology8(11)R251(2007)
Stein C,Caccamo M,Laird G,Leptin M

Dusty protein kinases: primary structure, gene evolution, tissue specific expression and unique features of the catalytic domain.
Biochimica et biophysica acta1759(11-12)562-72(2006 Nov-Dec)
Peng J,Dong W,Chen Y,Mo R,Cheng JF,Hui CC,Mohandas N,Huang CH

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DSTYK cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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