bmi1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bmi1b
Entrez Gene ID	394249
Full Name	bmi1 polycomb ring finger oncogene 1b
Synonyms	pcgf4b
General protein information	Preferred Names bmi1 polycomb ring finger oncogene 1b Names polycomb complex protein BMI-1-B B lymphoma Mo-MLV insertion region 1b polycomb group RING finger protein 4-B polycomb group ring finger 4 B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021479461.1	XP_021335136.1	polycomb complex protein BMI-1-B isoform X1
NM_001080751.1	NP_001074220.1	polycomb complex protein BMI-1-B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BMI1	XP_858578.1
Rattus norvegicus (Norway rat)	Bmi1	NP_001100838.1
Gallus gallus (chicken)	BMI1	NP_001007989.1
Mus musculus (house mouse)	Bmi1	NP_031578.2
Danio rerio (zebrafish)	bmi1b	NP_001074220.1
Homo sapiens (human)	COMMD3-BMI1	NP_001190991.1
	COMMD3	XP_002805629.1
Bos taurus (cattle)	BMI1	NP_001033161.1
Xenopus tropicalis (tropical clawed frog)	bmi1	NP_001015995.1

Gene Ontology
Bibliography

Related articles in PubMed

The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
Dupret B, V?lkel P, Le Bourhis X, Angrand PO
PloS one11(7)e0158700(2016)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development.
Mochida GH, Ganesh VS, de Michelena MI, Dias H, Atabay KD, Kathrein KL, Huang HT, Hill RS, Felie JM, Rakiec D, Gleason D, Hill AD, Malik AN, Barry BJ, Partlow JN, Tan WH, Glader LJ, Barkovich AJ, Dobyns WB, Zon LI, Walsh CA
Nature genetics44(11)1260-4(2012 Nov)

The zebrafish genes encoding the Polycomb repressive complex (PRC) 1.
Le Faou P, V?lkel P, Angrand PO
Gene475(1)10-21(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bmi1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmi1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03718	NM_001080751.1 Latest version!	Danio rerio bmi1 polycomb ring finger oncogene 1b (bmi1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa06914	XM_021479461.1 Latest version!	Danio rerio bmi1 polycomb ring finger oncogene 1b (bmi1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmi1b ( NM_001080751.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074220.1 Danio rerio bmi1 polycomb ring finger oncogene 1b (bmi1b), mRNA.

Price & Availability↑

CloneID	ODa03718
Clone ID Related Accession (Same CDS sequence)	NM_001080751.1
Accession Version	NM_001080751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Danio rerio(zebrafish)
Product	polycomb complex protein BMI-1-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC053151.1. On Mar 11, 2007 this sequence version replaced XM_001337466.1. ##Evidence-Data-START## Transcript exon combination :: BC053151.1, EL646426.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCACCGGA CAACGAGAAT CAAGATAACG GAGCTAAATC CTCATTTGAT GTGTGTTTTA 
TGTGGAGGAT ACTTCATTGA TGCGACCACT ATTGTTGAAT GTTTGCATTC GTTCTGCAAA 
ATGTGTATTG TCCGGTATTT GGAGACCAGC AAATATTGTC CTATTTGTGA TGTCCAAGTT 
CATAAAACAA AACCCCTCCT CAACATCAGG TCAGATAAGA CCCTTCAGGA CATTGTTTAC 
AAGCTGGTGC CTGGTTTGTT TAAAAATGAG ATGAAAAGGA GAAGGGATTT TTATGCTGAA 
CATCCCGTTG ATGCTACTAA TGGTTCCAAT GAGGACAGAG GAGAGGTGTC AGATGAAGAC 
AAGAGAATCA TTGCTGATGA TGAGATCATC AGTCTGTCCA TTGAGTTTTT TGATCAGAAA 
AAGCTTGATG GTAAAGATGG GGAAGAAAAA GAATCCACAA AAGAGGTGAC TGTAAAGCGT 
TACTTACAAT GCCCTGCAGC TATGACAGTA ATGCATCTCA GAAAATTTCT CAGAAGTAAA 
ATGGATATAC CATGTACCTT TCAGATTGAA GTTATGTATG AAGATGAGCC TTTGAAAGAT 
TATTACACTC TTATGGATAT TGCTTATATC TACACATGGA GAAGGAATGG GCCATTGCCT 
TTGAAGTACA GAGTTCGACC GGGCTGCAAA AAAATCAAGT TGAGCAGTCC CAGGAATGAT 
ATGAGTGGTG GCAGAAGGCC TGATACGGAA AGTGATTCCA GCAGTGACAA ACCAAACAGC 
CCGAGTATTG TTGCTGCCCC GTCTACATCA TCCTCTATGC CCAGCCCAAA CACCCCTGTC 
CAGTCTACAC ACCCCAGCTT CCCTCACATC TCCACCATTA ATGGAGTCTC TGCCAAAGTG 
GGCCACAATG GCCAAACACC TTTCAGCAGC AAAGTCTGCA AAACTTCTCA TAATGGCTCT 
AACTCTCTTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074220.1
CDS	114..1088
Translation	MHRTTRIKITELNPHLMCVLCGGYFIDATTIVECLHSFCKMCIVRYLETSKYCPICDVQVHKTKPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAEHPVDATNGSNEDRGEVSDEDKRIIADDEIISLSIEFFDQKKLDGKDGEEKESTKEVTVKRYLQCPAAMTVMHLRKFLRSKMDIPCTFQIEVMYEDEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPGCKKIKLSSPRNDMSGGRRPDTESDSSSDKPNSPSIVAAPSTSSSMPSPNTPVQSTHPSFPHISTINGVSAKVGHNGQTPFSSKVCKTSHNGSNSLG

Target ORF information:

RefSeq Version	NM_001080751.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio bmi1 polycomb ring finger oncogene 1b (bmi1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080751.1

ORF Insert Sequence:

ATGCACCGGA CAACGAGAAT CAAGATAACG GAGCTAAATC CTCATTTGAT GTGTGTTTTA 
TGTGGAGGAT ACTTCATTGA TGCGACCACT ATTGTTGAAT GTTTGCATTC GTTCTGCAAA 
ATGTGTATTG TCCGGTATTT GGAGACCAGC AAATATTGTC CTATTTGTGA TGTCCAAGTT 
CATAAAACAA AACCCCTCCT CAACATCAGG TCAGATAAGA CCCTTCAGGA CATTGTTTAC 
AAGCTGGTGC CTGGTTTGTT TAAAAATGAG ATGAAAAGGA GAAGGGATTT TTATGCTGAA 
CATCCCGTTG ATGCTACTAA TGGTTCCAAT GAGGACAGAG GAGAGGTGTC AGATGAAGAC 
AAGAGAATCA TTGCTGATGA TGAGATCATC AGTCTGTCCA TTGAGTTTTT TGATCAGAAA 
AAGCTTGATG GTAAAGATGG GGAAGAAAAA GAATCCACAA AAGAGGTGAC TGTAAAGCGT 
TACTTACAAT GCCCTGCAGC TATGACAGTA ATGCATCTCA GAAAATTTCT CAGAAGTAAA 
ATGGATATAC CATGTACCTT TCAGATTGAA GTTATGTATG AAGATGAGCC TTTGAAAGAT 
TATTACACTC TTATGGATAT TGCTTATATC TACACATGGA GAAGGAATGG GCCATTGCCT 
TTGAAGTACA GAGTTCGACC GGGCTGCAAA AAAATCAAGT TGAGCAGTCC CAGGAATGAT 
ATGAGTGGTG GCAGAAGGCC TGATACGGAA AGTGATTCCA GCAGTGACAA ACCAAACAGC 
CCGAGTATTG TTGCTGCCCC GTCTACATCA TCCTCTATGC CCAGCCCAAA CACCCCTGTC 
CAGTCTACAC ACCCCAGCTT CCCTCACATC TCCACCATTA ATGGAGTCTC TGCCAAAGTG 
GGCCACAATG GCCAAACACC TTTCAGCAGC AAAGTCTGCA AAACTTCTCA TAATGGCTCT 
AACTCTCTTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmi1b ( XM_021479461.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335136.1 Danio rerio bmi1 polycomb ring finger oncogene 1b (bmi1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06914
Clone ID Related Accession (Same CDS sequence)	XM_021479461.1
Accession Version	XM_021479461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	polycomb complex protein BMI-1-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGC ACCGGACAAC GAGAATCAAG ATAACGGAGC TAAATCCTCA TTTGATGTGT 
GTTTTATGTG GAGGATACTT CATTGATGCG ACCACTATTG TTGAATGTTT GCATTCGTTC 
TGCAAAATGT GCATTGTCCG GTATTTGGAG ACCAGCAAAT ATTGTCCTAT TTGTGATGTC 
CAAGTTCATA AAACAAAACC CCTCCTCAAC ATCAGGTCAG ATAAGACCCT TCAGGACATT 
GTTTACAAGC TGGTGCCTGG TTTGTTTAAA AATGAGATGA AAAGGAGAAG GGATTTTTAT 
GCTGAACATC CCGTTGATGC TACTAATGGT TCCAATGAGG ACAGAGGAGA GGTGTCAGAT 
GAAGACAAGA GAATCATTGC TGATGATGAG ATCATCAGTC TGTCCATTGA GTTTTTTGAT 
CAGAAAAAGC TTGATGGTAA AGATGGGGAA GAAAAAGAAT CCACAAAAGA GGTGACTGTA 
AAGCGTTACT TACAATGCCC TGCAGCTATG ACAGTAATGC ATCTCAGAAA ATTTCTTAGA 
AGTAAAATGG ATATACCATG TACCTTTCAG ATTGAAGTTA TGTATGAAGA TGAGCCTTTG 
AAAGATTATT ACACTCTTAT GGATATTGCT TATATTTACA CATGGAGAAG GAATGGGCCA 
TTGCCTTTGA AGTACAGAGT TCGACCGGGC TGCAAAAAAA TCAAGTTGAG CAGTCCCAGG 
AATGATATGA GTGGTGGCAG AAGGCCTGAT ACGGAAAGTG ATTCCAGCAG TGACAAACCA 
AACAGCCCGA GTATTGTTGC TGCCCCGTCT ACATCATCCT CTATGCCCAG CCCAAACACC 
CCTGTCCAGT CTACACACCC CAGCTTCCCT CACATCTCCA CCATTAATGG AGTCTCTGCC 
AAAGTGGGCC ACAATGGCCA AACACCTTTC AGCAGCAAAG TCTGCAAAAC TTCTCATAAT 
GGCTCTAACT CTCTTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335136.1
CDS	185..1165
Translation	MTMHRTTRIKITELNPHLMCVLCGGYFIDATTIVECLHSFCKMCIVRYLETSKYCPICDVQVHKTKPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAEHPVDATNGSNEDRGEVSDEDKRIIADDEIISLSIEFFDQKKLDGKDGEEKESTKEVTVKRYLQCPAAMTVMHLRKFLRSKMDIPCTFQIEVMYEDEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPGCKKIKLSSPRNDMSGGRRPDTESDSSSDKPNSPSIVAAPSTSSSMPSPNTPVQSTHPSFPHISTINGVSAKVGHNGQTPFSSKVCKTSHNGSNSLG

Target ORF information:

RefSeq Version	XM_021479461.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio bmi1 polycomb ring finger oncogene 1b (bmi1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479461.1

ORF Insert Sequence:

ATGACAATGC ACCGGACAAC GAGAATCAAG ATAACGGAGC TAAATCCTCA TTTGATGTGT 
GTTTTATGTG GAGGATACTT CATTGATGCG ACCACTATTG TTGAATGTTT GCATTCGTTC 
TGCAAAATGT GCATTGTCCG GTATTTGGAG ACCAGCAAAT ATTGTCCTAT TTGTGATGTC 
CAAGTTCATA AAACAAAACC CCTCCTCAAC ATCAGGTCAG ATAAGACCCT TCAGGACATT 
GTTTACAAGC TGGTGCCTGG TTTGTTTAAA AATGAGATGA AAAGGAGAAG GGATTTTTAT 
GCTGAACATC CCGTTGATGC TACTAATGGT TCCAATGAGG ACAGAGGAGA GGTGTCAGAT 
GAAGACAAGA GAATCATTGC TGATGATGAG ATCATCAGTC TGTCCATTGA GTTTTTTGAT 
CAGAAAAAGC TTGATGGTAA AGATGGGGAA GAAAAAGAAT CCACAAAAGA GGTGACTGTA 
AAGCGTTACT TACAATGCCC TGCAGCTATG ACAGTAATGC ATCTCAGAAA ATTTCTTAGA 
AGTAAAATGG ATATACCATG TACCTTTCAG ATTGAAGTTA TGTATGAAGA TGAGCCTTTG 
AAAGATTATT ACACTCTTAT GGATATTGCT TATATTTACA CATGGAGAAG GAATGGGCCA 
TTGCCTTTGA AGTACAGAGT TCGACCGGGC TGCAAAAAAA TCAAGTTGAG CAGTCCCAGG 
AATGATATGA GTGGTGGCAG AAGGCCTGAT ACGGAAAGTG ATTCCAGCAG TGACAAACCA 
AACAGCCCGA GTATTGTTGC TGCCCCGTCT ACATCATCCT CTATGCCCAG CCCAAACACC 
CCTGTCCAGT CTACACACCC CAGCTTCCCT CACATCTCCA CCATTAATGG AGTCTCTGCC 
AAAGTGGGCC ACAATGGCCA AACACCTTTC AGCAGCAAAG TCTGCAAAAC TTCTCATAAT 
GGCTCTAACT CTCTTGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Polycomb Group Protein Pcgf1 Is Dispensable in Zebrafish but Involved in Early Growth and Aging.
PloS one11(7)e0158700(2016)
Dupret B,V?lkel P,Le Bourhis X,Angrand PO

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

CHMP1A encodes an essential regulator of BMI1-INK4A in cerebellar development.
Nature genetics44(11)1260-4(2012 Nov)
Mochida GH,Ganesh VS,de Michelena MI,Dias H,Atabay KD,Kathrein KL,Huang HT,Hill RS,Felie JM,Rakiec D,Gleason D,Hill AD,Malik AN,Barry BJ,Partlow JN,Tan WH,Glader LJ,Barkovich AJ,Dobyns WB,Zon LI,Walsh CA

The zebrafish genes encoding the Polycomb repressive complex (PRC) 1.
Gene475(1)10-21(2011 Apr)
Le Faou P,V?lkel P,Angrand PO

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bmi1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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