st3gal5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	st3gal5
Entrez Gene ID	394230
Full Name	ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Synonyms	st3Gal-V,wu:fc08a12
General protein information	Preferred Names ST3 beta-galactoside alpha-2,3-sialyltransferase 5 Names lactosylceramide alpha-2,3-sialyltransferase GM3 synthase ganglioside GM3 synthase st3gal5-r1 zST3GalV-1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157139.4	XP_005157196.1	lactosylceramide alpha-2,3-sialyltransferase isoform X1
XM_005157139.3	XP_005157196.1	lactosylceramide alpha-2,3-sialyltransferase isoform X1
NM_199516.2	NP_955810.2	lactosylceramide alpha-2,3-sialyltransferase

Pathways from BioSystems

Homologies

	ST3GAL5	XP_001090769.2
Gallus gallus (chicken)	ST3GAL5	NP_001001192.1
Homo sapiens (human)	ST3GAL5	NP_003887.3
Mus musculus (house mouse)	St3gal5	NP_001030305.1
Rattus norvegicus (Norway rat)	St3gal5	NP_112627.2
Danio rerio (zebrafish)	st3gal5	NP_955810.2
Pan troglodytes (chimpanzee)	ST3GAL5	NP_001032378.1
Bos taurus (cattle)	ST3GAL5	NP_991376.2
Xenopus tropicalis (tropical clawed frog)	st3gal5	NP_001165301.1
Canis lupus familiaris (dog)	ST3GAL5	XP_854842.2

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Despic V, Dejung M, Gu M, Krishnan J, Zhang J, Herzel L, Straube K, Gerstein MB, Butter F, Neugebauer KM
Genome research27(7)1184-1194(2017 Jul)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Integrative view of ?2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.
Petit D, Teppa E, Mir AM, Vicogne D, Thisse C, Thisse B, Filloux C, Harduin-Lepers A
Molecular biology and evolution32(4)906-27(2015 Apr)

A mutation in a ganglioside biosynthetic enzyme, ST3GAL5, results in salt & pepper syndrome, a neurocutaneous disorder with altered glycolipid and glycoprotein glycosylation.
Boccuto L, Aoki K, Flanagan-Steet H, Chen CF, Fan X, Bartel F, Petukh M, Pittman A, Saul R, Chaubey A, Alexov E, Tiemeyer M, Steet R, Schwartz CE
Human molecular genetics23(2)418-33(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following st3gal5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the st3gal5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00861	NM_199516.2 Latest version!	Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa19362	XM_005157139.4 Latest version!	Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa19362	XM_005157139.3	Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

st3gal5 ( NM_199516.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955810.2 Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), mRNA.

Price & Availability↑

CloneID	ODa00861
Clone ID Related Accession (Same CDS sequence)	NM_199516.2
Accession Version	NM_199516.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Danio rerio(zebrafish)
Product	lactosylceramide alpha-2,3-sialyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC125822.1. On Nov 8, 2006 this sequence version replaced NM_199516.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC125822.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476759, SAMN01765705 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGAG TCATGAAACA ATCCAGCTGT TATTTCAGCA AACGGACGAT GATTCTTCTT 
CTGAGCTTGG CTTTGATGTC ACTGGCGTTT TTAAAGCTGC CTTCCTTTCA TACGGAACTA 
AAACCTGTGG AAGTGCCAGT AGACAATAAA TTCAGAAAGC GTGTCCATTC TCATGTCCGA 
GAGATACTAG ACAAAGAATG CAGACCTTCC TTTGCCCGGC AGAGAATGGT GACTGAACAT 
CATGGCTCCA CACCAACAAT AGATCCTTTT TTGAACAAGA ACATGAAGTT AGATAAACAA 
ATATTCCAGT ATCCTCCACC CTTTGGGTTC CTGGACATGA AGAACAAGCT TGAGGAAATC 
CTAAACCTCT TACCAGTTTC TTCTGAACAG AGGCTTGGGG AGCGAGATTG TCGACGCTGT 
GTAGTGGTTG GGAATGGAGG GATACTGAAA GGATTAGGAC TCGGACATCT ACTTAATCGT 
TTCGATATTA TCATCAGACT GAACAGTGGT CCTCTTCAAG ACTTCAGTGC AGATGTTGGG 
AACCGCACCA CTATCAGAAT GAGCTACCCT GAAAGCTGCC CGAAGGTCTG GGAAGATACG 
GATCCAGACC TCAAATATGT GGCTGTGATA TTCAAGTCTG TAGACTTCCA CTGGCTTCGT 
GCAATGATCA GCAGGACACC AGTTTCGCTG TGGGACAGGC TGTTTTTCTG GCAGAACGTG 
CCAATGAGCG TTCCAGTCAA GACCTCCCAG TTTCATCTCC TGAATCCCCA GATTATCCGT 
GAAATGGCTT TGGATCTGCT TAATTATCCC GAGCCTAAAA AACGGCTCTG GAGCTGGGAC 
CAGAACATCC CTACATTAGG GCTTACAGCT CTCAATCTGG CAACATACAT ATGTGACGAG 
GTGAGCCTAG CAGGCTTTGG CTATAACCTC AGCCAGAAAG AGGCTCCTCT TCATTACTAT 
GACAGCGTTC CCATGACAAC CATTTTAAAG GAAGCCATGC ACAATGTACA GAAAGAGACT 
GTTTTTCTCA AACGTTTGGT AGCCTCAGGC AGTATTACGG ACCTCACAGG TGGGATACAC 
TGCAGCTTCT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955810.2
CDS	239..1333
Translation	MRRVMKQSSCYFSKRTMILLLSLALMSLAFLKLPSFHTELKPVEVPVDNKFRKRVHSHVREILDKECRPSFARQRMVTEHHGSTPTIDPFLNKNMKLDKQIFQYPPPFGFLDMKNKLEEILNLLPVSSEQRLGERDCRRCVVVGNGGILKGLGLGHLLNRFDIIIRLNSGPLQDFSADVGNRTTIRMSYPESCPKVWEDTDPDLKYVAVIFKSVDFHWLRAMISRTPVSLWDRLFFWQNVPMSVPVKTSQFHLLNPQIIREMALDLLNYPEPKKRLWSWDQNIPTLGLTALNLATYICDEVSLAGFGYNLSQKEAPLHYYDSVPMTTILKEAMHNVQKETVFLKRLVASGSITDLTGGIHCSFC

Target ORF information:

RefSeq Version	NM_199516.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199516.2

ORF Insert Sequence:

ATGAGGAGAG TCATGAAACA ATCCAGCTGT TATTTCAGCA AACGGACGAT GATTCTTCTT 
CTGAGCTTGG CTTTGATGTC ACTGGCGTTT TTAAAGCTGC CTTCCTTTCA TACGGAACTA 
AAACCTGTGG AAGTGCCAGT AGACAATAAA TTCAGAAAGC GTGTCCATTC TCATGTCCGA 
GAGATACTAG ACAAAGAATG CAGACCTTCC TTTGCCCGGC AGAGAATGGT GACTGAACAT 
CATGGCTCCA CACCAACAAT AGATCCTTTT TTGAACAAGA ACATGAAGTT AGATAAACAA 
ATATTCCAGT ATCCTCCACC CTTTGGGTTC CTGGACATGA AGAACAAGCT TGAGGAAATC 
CTAAACCTCT TACCAGTTTC TTCTGAACAG AGGCTTGGGG AGCGAGATTG TCGACGCTGT 
GTAGTGGTTG GGAATGGAGG GATACTGAAA GGATTAGGAC TCGGACATCT ACTTAATCGT 
TTCGATATTA TCATCAGACT GAACAGTGGT CCTCTTCAAG ACTTCAGTGC AGATGTTGGG 
AACCGCACCA CTATCAGAAT GAGCTACCCT GAAAGCTGCC CGAAGGTCTG GGAAGATACG 
GATCCAGACC TCAAATATGT GGCTGTGATA TTCAAGTCTG TAGACTTCCA CTGGCTTCGT 
GCAATGATCA GCAGGACACC AGTTTCGCTG TGGGACAGGC TGTTTTTCTG GCAGAACGTG 
CCAATGAGCG TTCCAGTCAA GACCTCCCAG TTTCATCTCC TGAATCCCCA GATTATCCGT 
GAAATGGCTT TGGATCTGCT TAATTATCCC GAGCCTAAAA AACGGCTCTG GAGCTGGGAC 
CAGAACATCC CTACATTAGG GCTTACAGCT CTCAATCTGG CAACATACAT ATGTGACGAG 
GTGAGCCTAG CAGGCTTTGG CTATAACCTC AGCCAGAAAG AGGCTCCTCT TCATTACTAT 
GACAGCGTTC CCATGACAAC CATTTTAAAG GAAGCCATGC ACAATGTACA GAAAGAGACT 
GTTTTTCTCA AACGTTTGGT AGCCTCAGGC AGTATTACGG ACCTCACAGG TGGGATACAC 
TGCAGCTTCT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

st3gal5 ( XM_005157139.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157196.1 Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19362
Clone ID Related Accession (Same CDS sequence)	XM_005157139.4 , XM_005157139.3
Accession Version	XM_005157139.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lactosylceramide alpha-2,3-sialyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157139.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAG TCATGAAACA ATCCAGCTGT TATTTCAGCA AACGGACGAT GATTCTTCTT 
CTGAGCTTGG CTTTGATGTC ACTGGCGTTT TTAAAGCTGC CTTCCTTTCA TACGGAACTA 
AAACCTGTGG AAGTGCCTGT AGACAACAAA TTCAGAAAGC GTGTCCATTC TCATGTCCGA 
GAGATACTAG ACAAAGAATG CAGACCTTCC TTTGCCCGGC AGAGAATGGT GACTGAACAT 
CATGGCTCCA CACCAACAAT AGATCCTTTT TTGAACAAGA ACATGAAGTT AGATGAACAA 
ATATTCCAGT ATCCTCCACC CTTTGGGTTC CTGGACATGA AGAACAAGCT TGAGGAAATC 
CTAAACCTCT TACCAGTTTC TTCTGAACAG AGGCTTGGGG AGCGAGATTG TCGACGCTGT 
GTAGTGGTTG GGAATGGAGG GATACTGAAA GGATTAGGAC TCGGACATCT ACTTAATCGT 
TTCGATATTA TCATCAGACT GAACAGTGGT CCTCTTCAAG ACTTCAGTGC AGATGTTGGG 
AACCGCACCA CTATCAGAAT GAGCTACCCT GAAAGCTGCC CGAAGGTCTG GGAAGATACG 
GATCCAGACC TCAAATATGT GGCTGTGATA TTCAAGTCTG TAGACTTCCA CTGGCTTCGT 
GCAATGATCA GCAGGACACC AGTTTCGCTG TGGGACAGGC TGTTTTTCTG GCAGAACGTG 
CCAATGAGCG TTCCAGTCAA GACCTCCCAG TTTCATCTCC TGAATCCCCA GATTATCCGT 
GAGATGGCGT TGGATCTGCT TAATTATCCC GAGCCTAAAA AGCGGCTCTG GAGCTGGGAC 
CAGAACATCC CTACATTAGG GCTTACAGCT CTCAATCTGG CAACATACAT ATGTGACGAG 
GTGAGCCTAG CAGGCTTTGG CTATAACCTC AGCCAGAAAG AGGCTCCTCT TCATTACTAT 
GACAGCGTTC CCATGACAAC CATTTTAAAG GAAGCCATGC ACAATGTACA GAAAGAGACT 
GTTTTTCTCA AACGTTTGGT AGCCTCAGGC AGTATTACGG ACCTCACAGG TGGGATACAC 
TGCAGCTTCT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157196.1
CDS	825..1919
Translation	MRRVMKQSSCYFSKRTMILLLSLALMSLAFLKLPSFHTELKPVEVPVDNKFRKRVHSHVREILDKECRPSFARQRMVTEHHGSTPTIDPFLNKNMKLDEQIFQYPPPFGFLDMKNKLEEILNLLPVSSEQRLGERDCRRCVVVGNGGILKGLGLGHLLNRFDIIIRLNSGPLQDFSADVGNRTTIRMSYPESCPKVWEDTDPDLKYVAVIFKSVDFHWLRAMISRTPVSLWDRLFFWQNVPMSVPVKTSQFHLLNPQIIREMALDLLNYPEPKKRLWSWDQNIPTLGLTALNLATYICDEVSLAGFGYNLSQKEAPLHYYDSVPMTTILKEAMHNVQKETVFLKRLVASGSITDLTGGIHCSFC

Target ORF information:

RefSeq Version	XM_005157139.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157139.4

ORF Insert Sequence:

ATGAGGAGAG TCATGAAACA ATCCAGCTGT TATTTCAGCA AACGGACGAT GATTCTTCTT 
CTGAGCTTGG CTTTGATGTC ACTGGCGTTT TTAAAGCTGC CTTCCTTTCA TACGGAACTA 
AAACCTGTGG AAGTGCCTGT AGACAACAAA TTCAGAAAGC GTGTCCATTC TCATGTCCGA 
GAGATACTAG ACAAAGAATG CAGACCTTCC TTTGCCCGGC AGAGAATGGT GACTGAACAT 
CATGGCTCCA CACCAACAAT AGATCCTTTT TTGAACAAGA ACATGAAGTT AGATGAACAA 
ATATTCCAGT ATCCTCCACC CTTTGGGTTC CTGGACATGA AGAACAAGCT TGAGGAAATC 
CTAAACCTCT TACCAGTTTC TTCTGAACAG AGGCTTGGGG AGCGAGATTG TCGACGCTGT 
GTAGTGGTTG GGAATGGAGG GATACTGAAA GGATTAGGAC TCGGACATCT ACTTAATCGT 
TTCGATATTA TCATCAGACT GAACAGTGGT CCTCTTCAAG ACTTCAGTGC AGATGTTGGG 
AACCGCACCA CTATCAGAAT GAGCTACCCT GAAAGCTGCC CGAAGGTCTG GGAAGATACG 
GATCCAGACC TCAAATATGT GGCTGTGATA TTCAAGTCTG TAGACTTCCA CTGGCTTCGT 
GCAATGATCA GCAGGACACC AGTTTCGCTG TGGGACAGGC TGTTTTTCTG GCAGAACGTG 
CCAATGAGCG TTCCAGTCAA GACCTCCCAG TTTCATCTCC TGAATCCCCA GATTATCCGT 
GAGATGGCGT TGGATCTGCT TAATTATCCC GAGCCTAAAA AGCGGCTCTG GAGCTGGGAC 
CAGAACATCC CTACATTAGG GCTTACAGCT CTCAATCTGG CAACATACAT ATGTGACGAG 
GTGAGCCTAG CAGGCTTTGG CTATAACCTC AGCCAGAAAG AGGCTCCTCT TCATTACTAT 
GACAGCGTTC CCATGACAAC CATTTTAAAG GAAGCCATGC ACAATGTACA GAAAGAGACT 
GTTTTTCTCA AACGTTTGGT AGCCTCAGGC AGTATTACGG ACCTCACAGG TGGGATACAC 
TGCAGCTTCT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

st3gal5 ( XM_005157139.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157196.1 Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19362
Clone ID Related Accession (Same CDS sequence)	XM_005157139.4 , XM_005157139.3
Accession Version	XM_005157139.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lactosylceramide alpha-2,3-sialyltransferase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157139.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGAG TCATGAAACA ATCCAGCTGT TATTTCAGCA AACGGACGAT GATTCTTCTT 
CTGAGCTTGG CTTTGATGTC ACTGGCGTTT TTAAAGCTGC CTTCCTTTCA TACGGAACTA 
AAACCTGTGG AAGTGCCTGT AGACAACAAA TTCAGAAAGC GTGTCCATTC TCATGTCCGA 
GAGATACTAG ACAAAGAATG CAGACCTTCC TTTGCCCGGC AGAGAATGGT GACTGAACAT 
CATGGCTCCA CACCAACAAT AGATCCTTTT TTGAACAAGA ACATGAAGTT AGATGAACAA 
ATATTCCAGT ATCCTCCACC CTTTGGGTTC CTGGACATGA AGAACAAGCT TGAGGAAATC 
CTAAACCTCT TACCAGTTTC TTCTGAACAG AGGCTTGGGG AGCGAGATTG TCGACGCTGT 
GTAGTGGTTG GGAATGGAGG GATACTGAAA GGATTAGGAC TCGGACATCT ACTTAATCGT 
TTCGATATTA TCATCAGACT GAACAGTGGT CCTCTTCAAG ACTTCAGTGC AGATGTTGGG 
AACCGCACCA CTATCAGAAT GAGCTACCCT GAAAGCTGCC CGAAGGTCTG GGAAGATACG 
GATCCAGACC TCAAATATGT GGCTGTGATA TTCAAGTCTG TAGACTTCCA CTGGCTTCGT 
GCAATGATCA GCAGGACACC AGTTTCGCTG TGGGACAGGC TGTTTTTCTG GCAGAACGTG 
CCAATGAGCG TTCCAGTCAA GACCTCCCAG TTTCATCTCC TGAATCCCCA GATTATCCGT 
GAGATGGCGT TGGATCTGCT TAATTATCCC GAGCCTAAAA AGCGGCTCTG GAGCTGGGAC 
CAGAACATCC CTACATTAGG GCTTACAGCT CTCAATCTGG CAACATACAT ATGTGACGAG 
GTGAGCCTAG CAGGCTTTGG CTATAACCTC AGCCAGAAAG AGGCTCCTCT TCATTACTAT 
GACAGCGTTC CCATGACAAC CATTTTAAAG GAAGCCATGC ACAATGTACA GAAAGAGACT 
GTTTTTCTCA AACGTTTGGT AGCCTCAGGC AGTATTACGG ACCTCACAGG TGGGATACAC 
TGCAGCTTCT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157196.1
CDS	830..1924
Translation	MRRVMKQSSCYFSKRTMILLLSLALMSLAFLKLPSFHTELKPVEVPVDNKFRKRVHSHVREILDKECRPSFARQRMVTEHHGSTPTIDPFLNKNMKLDEQIFQYPPPFGFLDMKNKLEEILNLLPVSSEQRLGERDCRRCVVVGNGGILKGLGLGHLLNRFDIIIRLNSGPLQDFSADVGNRTTIRMSYPESCPKVWEDTDPDLKYVAVIFKSVDFHWLRAMISRTPVSLWDRLFFWQNVPMSVPVKTSQFHLLNPQIIREMALDLLNYPEPKKRLWSWDQNIPTLGLTALNLATYICDEVSLAGFGYNLSQKEAPLHYYDSVPMTTILKEAMHNVQKETVFLKRLVASGSITDLTGGIHCSFC

Target ORF information:

RefSeq Version	XM_005157139.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ST3 beta-galactoside alpha-2,3-sialyltransferase 5 (st3gal5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157139.3

ORF Insert Sequence:

ATGAGGAGAG TCATGAAACA ATCCAGCTGT TATTTCAGCA AACGGACGAT GATTCTTCTT 
CTGAGCTTGG CTTTGATGTC ACTGGCGTTT TTAAAGCTGC CTTCCTTTCA TACGGAACTA 
AAACCTGTGG AAGTGCCTGT AGACAACAAA TTCAGAAAGC GTGTCCATTC TCATGTCCGA 
GAGATACTAG ACAAAGAATG CAGACCTTCC TTTGCCCGGC AGAGAATGGT GACTGAACAT 
CATGGCTCCA CACCAACAAT AGATCCTTTT TTGAACAAGA ACATGAAGTT AGATGAACAA 
ATATTCCAGT ATCCTCCACC CTTTGGGTTC CTGGACATGA AGAACAAGCT TGAGGAAATC 
CTAAACCTCT TACCAGTTTC TTCTGAACAG AGGCTTGGGG AGCGAGATTG TCGACGCTGT 
GTAGTGGTTG GGAATGGAGG GATACTGAAA GGATTAGGAC TCGGACATCT ACTTAATCGT 
TTCGATATTA TCATCAGACT GAACAGTGGT CCTCTTCAAG ACTTCAGTGC AGATGTTGGG 
AACCGCACCA CTATCAGAAT GAGCTACCCT GAAAGCTGCC CGAAGGTCTG GGAAGATACG 
GATCCAGACC TCAAATATGT GGCTGTGATA TTCAAGTCTG TAGACTTCCA CTGGCTTCGT 
GCAATGATCA GCAGGACACC AGTTTCGCTG TGGGACAGGC TGTTTTTCTG GCAGAACGTG 
CCAATGAGCG TTCCAGTCAA GACCTCCCAG TTTCATCTCC TGAATCCCCA GATTATCCGT 
GAGATGGCGT TGGATCTGCT TAATTATCCC GAGCCTAAAA AGCGGCTCTG GAGCTGGGAC 
CAGAACATCC CTACATTAGG GCTTACAGCT CTCAATCTGG CAACATACAT ATGTGACGAG 
GTGAGCCTAG CAGGCTTTGG CTATAACCTC AGCCAGAAAG AGGCTCCTCT TCATTACTAT 
GACAGCGTTC CCATGACAAC CATTTTAAAG GAAGCCATGC ACAATGTACA GAAAGAGACT 
GTTTTTCTCA AACGTTTGGT AGCCTCAGGC AGTATTACGG ACCTCACAGG TGGGATACAC 
TGCAGCTTCT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic RNA-protein interactions underlie the zebrafish maternal-to-zygotic transition.
Genome research27(7)1184-1194(2017 Jul)
Despic V,Dejung M,Gu M,Krishnan J,Zhang J,Herzel L,Straube K,Gerstein MB,Butter F,Neugebauer KM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Integrative view of ?2,3-sialyltransferases (ST3Gal) molecular and functional evolution in deuterostomes: significance of lineage-specific losses.
Molecular biology and evolution32(4)906-27(2015 Apr)
Petit D,Teppa E,Mir AM,Vicogne D,Thisse C,Thisse B,Filloux C,Harduin-Lepers A

A mutation in a ganglioside biosynthetic enzyme, ST3GAL5, results in salt & pepper syndrome, a neurocutaneous disorder with altered glycolipid and glycoprotein glycosylation.
Human molecular genetics23(2)418-33(2014 Jan)
Boccuto L,Aoki K,Flanagan-Steet H,Chen CF,Fan X,Bartel F,Petukh M,Pittman A,Saul R,Chaubey A,Alexov E,Tiemeyer M,Steet R,Schwartz CE

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st3gal5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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会社情報